Compound dietary composition for improving microcirculation and preparation method thereof

Abstract

The invention relates to the field of functional health food, and provides a compound dietary composition for improving microcirculation and a preparation method thereof. The composite dietary composition for improving microcirculation comprises the following components (in parts by weight): freeze-dried fruit and vegetable composite powder, European blueberry concentrated powder, sophora flower bud powder, (3R, 3 'R)-dihydroxy-beta-carotene, lutein microcapsule powder, matcha powder, fermented ginger powder and multivitamins. The freeze-dried fruit and vegetable composite powder, the European blueberry concentrated powder, the sophora flower bud powder and the (3R, 3 'R)-dihydroxy-beta-carotene are compounded, and the matcha powder, the multivitamins, the fermented ginger powder and the xanthophyll micro-capsule powder are added, so that the eye cream is rich in nutritional ingredients and has the effects of regulating microcirculation, promoting capillary softening and promoting eye microcirculation.

Description

Technical Field

[0001] The present application relates to the field of functional health foods, and in particular to a composite dietary composition for improving microcirculation and a preparation method thereof. Background Art

[0002] All organs of the human body have microcirculation, and when microcirculation is impaired, it can manifest as a variety of symptoms. Among them, the eye is rich in blood vessels, which is a microcosm of the microvasculature of the whole body and can reflect the condition of the microvasculature of the whole body. If the eye microcirculation is impaired, symptoms such as dry and itchy eyes / tears in the wind / tears due to photophobia, sore eyes / swollen / stinging eyes, congested eyes / red bloodshot eyes, decreased vision / blurred vision, obvious dark circles, and easy eye fatigue may occur.

[0003] In the related art, some commercially available dietary products mainly add lutein esters as functional ingredients to promote and maintain good ocular microcirculation. However, the content of lutein esters in food is very low, and it does not exist in plasma or in the macular area of ​​the optic retina. Moreover, after entering the human body, lutein esters need to be decomposed and converted into free lutein before being absorbed by the human body. The deesterification requires the help of fatty acid enzymatic cleavage process, and this complex enzymatic cleavage process varies significantly among individuals, so it has a greater impact on the digestion and absorption effect. Summary of the invention

[0004] In view of the above-mentioned deficiencies in the prior art, the purpose of the present application is to provide a composite dietary composition for improving microcirculation and a preparation method thereof. The composite dietary composition for improving microcirculation of the present application is compounded with freeze-dried fruit and vegetable composite powder, European blueberry concentrated powder, Sophora japonica powder, (3R, 3'R)-dihydroxy-β-carotene in a specific proportion, and is added with matcha powder, multivitamins, fermented ginger powder and lutein microcapsule powder. It contains rich nutrients, and proper consumption can supplement the body with the required nutrients and maintain the body's nutritional balance. At the same time, it has a good effect of adjusting microcirculation, promoting capillary softening, and promoting eye microcirculation.

[0005] In order to achieve the above-mentioned invention object, the technical solution adopted in this application is as follows: In a first aspect, the present application provides a composite dietary composition for improving microcirculation, comprising the following components: Freeze-dried fruit and vegetable compound powder, European blueberry concentrated powder, Sophora japonica rice powder, (3R,3'R)-dihydroxy-β-carotene, lutein microcapsule powder, matcha powder, fermented ginger powder and multivitamins; The lutein microcapsule powder comprises the following components: lutein, maltooligosaccharide, octenylsuccinate monoarabic gum ester, vitamin E, silicon dioxide and ascorbyl palmitate; The preparation steps of lutein microcapsule powder are as follows: The composite emulsifier is dissolved in water to prepare a first solution, and then octenyl succinic acid monoarabic gum ester is added to the first solution and mixed to obtain a second solution; Add ascorbyl palmitate to oil and heat to dissolve to obtain a third solution, add lutein, vitamin E and silicon dioxide to the third solution to disperse and homogenize to obtain a fourth solution; Splitting the second solution into a first aqueous phase and a second aqueous phase; Splitting the fourth solution into a first oil phase and a second oil phase; Adding the first water phase into the first oil phase at a first speed for shear emulsification to obtain a first emulsion; adding the second aqueous phase into the first emulsion at a second speed for shear emulsification to obtain a second emulsion; adding the second oil phase to the second emulsion at a third speed for shear emulsification to obtain a third emulsion; wherein the first speed is lower than the third speed, and the third speed is lower than the second speed; The third emulsion is subjected to high pressure homogenization and spray drying to obtain lutein microcapsule powder.

[0006] In a second aspect, the present application provides a method for preparing the composite dietary composition for improving microcirculation according to the first aspect, comprising: Freeze-dried fruit and vegetable composite powder, European blueberry concentrated powder, Sophora japonica rice powder, (3R, 3'R)-dihydroxy-β-carotene, lutein microcapsule powder, matcha powder, fermented ginger powder and complex vitamins are mixed with a tablet flow aid, and tablets are formed to obtain the product.

[0007] The beneficial effects of this application include at least: The composite dietary composition for improving microcirculation provided in the embodiment of the present application is compounded by freeze-dried fruit and vegetable composite powder, European blueberry concentrated powder, Sophora japonica powder, (3R, 3'R)-dihydroxy-β-carotene, and added with matcha powder, multivitamins, fermented ginger powder and lutein microcapsule powder, which contains rich nutrients. Appropriate consumption can supplement the body with the required nutrients and maintain the body's nutritional balance. At the same time, it can adjust microcirculation, promote capillary softening, and promote eye microcirculation. In addition, the lutein microcapsule powder used in the embodiment of the present application has good stability and sustained release, and the effective sustained-release component lutein can be directly absorbed by the human body, and has a high bioavailability; at the same time, by compounding the lutein microcapsule powder and (3R, 3'R)-dihydroxy-β-carotene in a specific ratio, the two have a synergistic effect, which can further improve the bioavailability of lutein and (3R, 3'R)-dihydroxy-β-carotene. DETAILED DESCRIPTION

[0008] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present application more clearly understood, the present application is further described in detail in conjunction with the embodiments below. It should be understood that the specific embodiments described herein are only used to explain the present application, but the implementation methods of the present application are not limited thereto.

[0009] Unless otherwise defined, the technical terms used in the following examples have the same meanings as those generally understood by those skilled in the art to which this application belongs. The experimental reagents used in the following examples, unless otherwise specified, are all conventional biochemical reagents and can be obtained through commercial channels; the amounts of experimental reagents, unless otherwise specified, are the amounts of reagents used in conventional experimental operations; and the experimental methods, unless otherwise specified, are all conventional methods.

[0010] In a first aspect, this embodiment provides a composite dietary composition for improving microcirculation, comprising the following components: Freeze-dried fruit and vegetable compound powder, European blueberry concentrated powder, Sophora japonica rice powder, (3R,3'R)-dihydroxy-β-carotene, lutein microcapsule powder, matcha powder, fermented ginger powder and multivitamins; The lutein microcapsule powder comprises the following components: lutein, maltooligosaccharide, octenylsuccinate monoarabic gum ester, vitamin E, silicon dioxide and ascorbyl palmitate; The preparation steps of lutein microcapsule powder are as follows: S1, dissolving the composite emulsifier in water to prepare a first solution, and then adding octenyl succinic acid monoarabic gum ester to the first solution and mixing them to obtain a second solution; S2, adding ascorbyl palmitate to oil and heating to dissolve, to obtain a third solution, adding lutein, vitamin E and silicon dioxide to the third solution for dispersion and homogenization, to obtain a fourth solution; S3, splitting the second solution into a first aqueous phase and a second aqueous phase; splitting the fourth solution into a first oil phase and a second oil phase; S4, adding the first water phase into the first oil phase at a first speed for shear emulsification to obtain a first emulsion; S5, adding the second aqueous phase into the first emulsion at a second speed for shear emulsification to obtain a second emulsion; S6, adding the second oil phase to the second emulsion at a third speed for shear emulsification to obtain a third emulsion; wherein the first speed is lower than the third speed, and the third speed is lower than the second speed; S7. High-pressure homogenization and spray drying are performed on the third emulsion to obtain lutein microcapsule powder.

[0011] In the related art, most of the lutein esters are directly applied to functional foods and health foods, or the lutein esters are prepared into lutein ester microcapsule powders, and then the lutein ester microcapsule powders are applied to functional foods and health foods. However, lutein esters are fatty acid esters of lutein, which are formed by esterification reaction between lutein and fatty acids. The content in food is very small, and it does not exist in plasma and retinal macular area. After entering the human intestine, it needs to be decomposed and converted into free form before it can be absorbed by the human body. In this process, it is necessary to use the fatty acid enzymatic cleavage process to complete deesterification, and this complex process varies significantly among individuals, thus affecting the absorption effect. Therefore, when lutein esters or lutein ester microcapsule powders are applied to functional foods and health foods, the effect of human absorption varies greatly, and the human digestion and absorption process is complex and the bioavailability is low.

[0012] Lutein is a free structure (i.e., exists in a free form), and is abundant in food (such as marigolds, etc.), exists in plasma and the macula of the retina, and can be directly absorbed by the human body. In the embodiment of the present application, by preparing lutein into lutein microcapsule powder, not only can the absorption difference of lutein between different individuals be reduced, but also the lutein microcapsule powder can release the lutein therein through sustained release after entering the human body, and the released lutein can be directly absorbed and utilized by the human body, which simplifies the absorption process of lutein and improves the bioavailability of lutein.

[0013] In some embodiments, in the above step S1, the composite emulsifier is a combination of mealworm protein, maltooligosaccharide and Tween.

[0014] As an example, mealworm protein can be prepared by the following steps: dry the mealworm powder at 60 °C to remove moisture, crush the dried mealworm powder, pass it through a 60-mesh sieve, and then use petroleum ether for degreasing to obtain fat-free insect powder. The fat-free insect powder is mixed and stirred with a 2 mol / L NaOH solution at a volume ratio of 1:20 for 4 h, then centrifuged at a speed of 5000 r / min for 15 min, and the supernatant is diluted with anhydrous ethanol and allowed to stand for 2 h. After centrifugation for 15 min, the supernatant is extracted, adjusted to pH 4.0 with a 2 mol / L hydrochloric acid solution, and then the protein is decontaminated with anhydrous ethanol. After that, the decontaminated protein is freeze-dried to obtain mealworm protein. The obtained mealworm protein has good water solubility.

[0015] Tween (also known as polysorbate) is a non-ionic surfactant. Tween and mealworm protein have a synergistic effect. During the shear emulsification process of the water phase and the oil phase, Tween can quickly migrate to the oil-water interface, reduce the surface tension, and enable the oil droplets to quickly disperse in the water phase to form an initial emulsion system; the mealworm protein is then adsorbed on the surface of the oil droplets and works together with the non-ionic surfactant to further reduce the interfacial energy. The mealworm protein forms an interfacial film with a certain thickness and mechanical strength on the surface of the oil droplets, but there may be some weak points, and Tween can just fill these weak areas, and combine with the mealworm protein through hydrophobic interactions, hydrogen bonds, etc., to enhance the overall strength and stability of the interfacial film, and prevent the aggregation of oil droplets and emulsion demulsification.

[0016] Mealworm protein and maltose have a synergistic effect. During the shear emulsification process of the water phase and the oil phase, after the mealworm protein is adsorbed on the surface of the oil droplet, maltose can interact with the mealworm protein, such as covalent bonding or physical adsorption, to form a hydration layer around the protein molecules of the mealworm protein, increase the effective volume of the protein molecules, and further increase the steric hindrance effect, making it more difficult for the oil droplets to approach and aggregate. In addition, maltose can adjust the conformation and stability of mealworm protein, so that it can better maintain its emulsification properties under different environmental conditions (such as different pH values, temperatures and ionic strengths). For example, under some acidic conditions, oligosaccharides can protect the structure of the protein and prevent it from denaturing, thereby maintaining the normal adsorption and emulsification of the protein at the oil-water interface.

[0017] Tween and maltooligosaccharide have a synergistic effect. In the shear emulsification process of the water phase and the oil phase, maltooligosaccharide has a certain hydrophilicity and can interact with the hydrophilic group of Tween to adjust the hydrophilicity of the compound system, so that the compound emulsifier can play a better role in different oil-water ratios and system compositions, and improve the adaptability and versatility of the emulsifier. Secondly, maltooligosaccharide can promote the adsorption and arrangement of Tween at the oil-water interface, making the distribution of surfactant molecules on the interface more uniform, thereby more effectively reducing surface tension and improving emulsification efficiency.

[0018] By compounding the three components of mealworm protein, oligomaltose and Tween to form a composite emulsifier, a more compact, stable and elastic composite interface film can be formed at the oil-water interface, which greatly improves the emulsification ability, makes the particle size of the emulsion smaller and more evenly distributed, and significantly enhances the stability of the emulsion. Under different environmental conditions such as pH value, temperature, ionic strength, etc., mealworm protein, oligomaltose and Tween can complement and synergize each other, so that the composite emulsifier has a wider range of applications and can better resist the influence of external factors on the stability of the emulsion. At the same time, by introducing the above-mentioned composite emulsifier into octenyl succinic acid monoarabic gum ester, it can play an excellent emulsifying role in the shear emulsification process of the water phase and the oil phase, and can also give the product additional functions, for example, mealworm protein can provide nutrition, oligomaltose has the functions of prebiotics, and can also make lutein microcapsule powder easier to be absorbed by the human body, thereby improving its bioavailability. In addition, the amount of emulsifier used can be reduced, which is conducive to reducing the preparation cost.

[0019] By introducing small molecule components (especially maltooligosaccharides) as wall materials into octenyl succinic acid monoarabic gum ester, these small molecule components themselves have a certain emulsification stability, which can make the subsequent shear emulsification process easier to carry out and enable the shear emulsification process to be carried out at a lower temperature.

[0020] In some embodiments, in the above step S1, the mass ratio of octenyl succinic acid monoarabic gum ester to the composite emulsifier is 1:0.1 to 0.5. Exemplarily, the mass ratio of octenyl succinic acid monoarabic gum ester to the composite emulsifier can be 1:0.1, 1:0.2, 1:0.3, 1:0.4 or 1:0.5, etc.

[0021] In some embodiments, in the steps of preparing the first emulsion, the second emulsion and the third emulsion, the temperature is controlled to be 25-45° C. and the pH value is controlled to be 4-7. In this way, the compound system of mealworm protein, oligomaltose and Tween can remain stable in the shear emulsification system, thereby improving the stability of the prepared emulsion.

[0022] In some embodiments, in the above step S2, ascorbyl palmitate can be added to deep-sea fish oil, linseed oil or vegetable oil and heated to dissolve to obtain a third solution, and then lutein, vitamin E and silicon dioxide are added to the third solution. The vegetable oil can be a vegetable oil commonly used in the art, specifically soybean oil, sunflower oil, peanut oil, corn oil, or a mixture of several of them in any proportion.

[0023] The third solution is formed by first heating ascorbyl palmitate into oil and dissolving it, and then adding lutein, vitamin E and silicon dioxide to the third solution, and dispersing and homogenizing the solution under the conditions of controlling the temperature at 40-70°C and the rotation speed at 10000-15000 r / min, so that the lutein can be fully and evenly dispersed in the third solution to form a uniform and stable oil phase system (i.e., the fourth solution).

[0024] Among them, ascorbyl palmitate has both emulsifying and antioxidant effects, which can promote the uniform dispersion of lutein in the oil phase system. At the same time, when used in combination with vitamin E, it has a synergistic antioxidant effect, which helps to delay the loss of part or all of the activity of lutein due to oxidation. In addition, the porous structure of silica can provide sufficient adsorption sites, so that lutein can be attached to these adsorption sites, which can effectively prevent the aggregation of lutein molecules due to mutual attraction, thereby further improving the uniform dispersion of lutein and forming a uniform and stable oil phase system.

[0025] In the above steps S3 to S6, by first splitting the second solution into the first aqueous phase and the second aqueous phase, and splitting the fourth solution into the first oil phase and the second oil phase, and then emulsifying in steps, and controlling the order and speed of adding the aqueous phase and the oil phase and the shear speed in each step, the oil phase and the aqueous phase in the emulsion system can be fully mixed during the emulsification process, thereby improving the stability of the emulsion and the uniformity of the particle size distribution, and at the same time improving the preparation efficiency of the emulsion.

[0026] In some embodiments, in the above step S4, the first aqueous phase can be added to the first oil phase at a rate of 1% to 2% of the total mass of the first system per minute, and shear emulsification is performed under a shear speed of 5000 to 10000 r / min to obtain a first emulsion; wherein the total mass of the first system refers to the sum of the mass of the first aqueous phase and the mass of the first oil phase. Exemplarily, the shear speed can be 5000 r / min, 8000 r / min or 10000 r / min, etc.; the shear time is 3 to 5 minutes, for example, 3, 4 or 5 minutes, etc.

[0027] In some embodiments, in the above step S5, the second aqueous phase can be added to the first emulsion at a rate of 3% to 5% of the total mass of the second system per minute, and shear emulsification is performed under a shear speed of 10000 to 25000 r / min to obtain a second emulsion; wherein the total mass of the second system refers to the sum of the mass of the second aqueous phase and the mass of the first emulsion. Exemplarily, the shear speed can be 10000 r / min, 12000 r / min, 15000 r / min, 20000 r / min or 25000 r / min, etc.; the shear time is 10 to 20 minutes, for example, 10, 15 or 20 minutes, etc.

[0028] In some embodiments, in the above step S6, the second oil phase can be added to the second emulsion at a rate of 1% to 3% of the total mass of the third system per minute, and shear emulsification is performed under a shear speed of 10000 to 25000 r / min to obtain a third emulsion; wherein the total mass of the third system refers to the sum of the mass of the second oil phase and the mass of the second emulsion. Exemplarily, the shear speed can be 10000 r / min, 12000 r / min, 15000 r / min, 20000 r / min or 25000 r / min, etc.; the shear time is 10 to 20 minutes, for example, 10, 15 or 20 minutes, etc.

[0029] In some embodiments, in the above step S7, the third emulsion obtained in the above step S6 is subjected to high pressure homogenization, and the homogenization pressure is 30-120 Mpa, for example, 30 Mpa, 40 Mpa, 50 Mpa, 60 Mpa, 70 Mpa, 80 Mpa, 90 Mpa, 100 Mpa, 110 Mpa or 120 Mpa, etc. Thereafter, spray drying is performed, and the inlet air temperature of the spray drying is controlled at 150-170°C, for example, it can be controlled at 150°C, 155°C, 160°C, 165°C or 170°C, etc. The outlet air temperature of the spray drying is controlled at 30-80°C, for example, it can be controlled at 30°C, 40°C, 50°C, 60°C, 70°C or 80°C, etc.

[0030] The lutein microcapsule powder prepared in the above implementation mode is an O / W type microcapsule powder, which has cold water dispersibility, good rehydration stability, high embedding rate, good sustained release performance and high bioavailability.

[0031] The composite dietary composition for improving microcirculation provided in the embodiment of the present application is compounded with freeze-dried fruit and vegetable composite powder, European blueberry concentrated powder, Sophora japonica rice powder, (3R, 3'R)-dihydroxy-β-carotene, and is added with matcha powder, complex vitamins, fermented ginger powder and lutein microcapsule powder. It contains rich nutrients. A proper amount of consumption can supplement the body with the required nutrients and maintain the body's nutritional balance. At the same time, it has a good effect of adjusting microcirculation, promoting capillary softening, and promoting eye microcirculation.

[0032] In some embodiments, the lutein microcapsule powder includes the following components in parts by weight: 10-20 parts of lutein, 1-100 parts of oligomaltose, 10-50 parts of octenyl succinate monoarabic gum ester, 5-7 parts of vitamin E, 5-10 parts of silicon dioxide, and 1-2 parts of ascorbyl palmitate. The diameter of the lutein microcapsule powder prepared by the preparation method of the embodiment of the present application is 1-100 nm, and the wall thickness is 0.5-150 nm.

[0033] In some embodiments, the preparation steps of the fermented ginger powder are as follows: Step 1: Wash, crush, squeeze and filter the ginger to obtain ginger juice; Step 2: sterilize the ginger juice, inoculate the fermentation bacteria, and ferment at 25-35° C. for at least 48 hours to obtain a ginger fermentation product; Step 3: Dry the fermented ginger to obtain fermented ginger powder.

[0034] In some embodiments, in the above step 1, the ginger can be first washed, peeled, and sliced ​​to obtain ginger slices, and then the ginger slices can be crushed, squeezed, and filtered to obtain ginger juice.

[0035] In some embodiments, in the above step 2, the ginger juice obtained in the above step 1 is placed in a stainless steel container, sealed, placed in an oven, and pasteurized at a temperature of 80-85°C. After that, it is taken out and cooled to room temperature, inoculated with fermentation bacteria, and fermented at 25-35°C for at least 48 hours to obtain ginger fermentation.

[0036] The fermentation strain may be one or a combination of Candida utilis, Acetobacter or Aspergillus oryzae. For example, the fermentation strain may be any one of Candida utilis, Acetobacter or Aspergillus oryzae; it may also be a combination of Candida utilis and Aspergillus oryzae, or a combination of Candida utilis and Acetobacter, or a combination of Acetobacter and Aspergillus oryzae; it may also be a combination of Candida utilis, Acetobacter and Aspergillus oryzae.

[0037] Candida utilis can utilize sugars, nitrogen sources and other substances in ginger for growth and fermentation, producing rich nutrients such as protein and vitamins, which can improve the nutritional value of ginger fermented products. It can also produce some flavor substances to improve the flavor of the product.

[0038] Acetobacter can produce some enzymes that can break down macromolecules in ginger such as proteins and polysaccharides, making the nutrients in ginger easier to be absorbed and utilized by the human body; at the same time, fermentation may also produce some new biologically active substances, such as certain antioxidants, probiotics, etc., thereby improving the nutritional value and health benefits of ginger products.

[0039] Aspergillus oryzae can produce a variety of enzymes, such as protease, amylase, saccharifying enzyme, etc., which can break down macromolecules such as protein and starch in ginger into small molecule peptides, amino acids, glucose, etc., which is beneficial to improve the fermentation efficiency of ginger and the quality of the product, and can be used in the production of ginger fermented seasonings and other products.

[0040] Experimental studies have found that when a combination of Candida utilis, Acetobacter and Aspergillus oryzae is used as a fermentation strain to ferment ginger juice, and the ratio of the number of live bacteria of Candida utilis, Acetobacter and Aspergillus oryzae is 10:2~5:1~2, the spiciness and slight bitterness of ginger can be effectively reduced, thereby improving the taste. In addition, using a combination of the above three strains to ferment ginger juice can not only improve the fermentation effect, but also better maintain the antioxidant activity of components such as 6-gingerol and gingerol in ginger.

[0041] Optionally, the mass ratio of the fermentation bacteria to the ginger juice is 1:100-150. For example, the mass ratio of the fermentation bacteria to the ginger juice can be 1:100, 1:110, 1:120, 1:130, 1:140 or 1:150, etc.

[0042] In some embodiments, the preparation steps of the above-mentioned Sophora japonica rice powder are as follows: Step 1, crushing the dried Sophora japonica seeds and then subjecting them to a supercritical carbon dioxide enzyme inactivation treatment to obtain an enzyme inactivation product; Step 2, adding the inactivated enzyme to an extraction solvent for extraction and separation to obtain an organic phase containing the effective ingredients of Sophora japonica seed; wherein the extraction solvent comprises the following components in parts by weight: 10-40 parts of polyvinyl alcohol, 5-40 parts of acidic inorganic salt, 70-90 parts of water and 2-10 parts of chitosan; Step 3, the organic phase is concentrated under reduced pressure to precipitate crystals to obtain Sophora japonica powder.

[0043] In some embodiments, in the above step 1, the solid-liquid ratio of the enzyme-inactivated Sophora japonica flower to the extraction solvent is 1:6-10; for example, it can be 1:6, 1:7, 1:8, 1:9 or 1:10, etc.

[0044] In some embodiments, in the above step 1, the extraction conditions are: the extraction time is 10 to 15 minutes (for example, it can be 10, 12 or 15 minutes, etc.), the extraction temperature is 25 to 70°C (for example, it can be 25°C, 30°C, 40°C, 50°C, 60°C or 70°C, etc.), and the pH value is 4 to 6 (for example, it can be 4, 4.5, 5, 5.5 or 6, etc.).

[0045] In some embodiments, in the above step 2, the acidic inorganic salt may be ammonium sulfate or the like.

[0046] A three-phase aqueous system is formed by compounding polyvinyl alcohol, an acidic inorganic salt, chitosan and water, wherein polyvinyl alcohol can form a two-phase aqueous system with the acidic inorganic salt and chitosan respectively, and the acidic inorganic salt and chitosan can also form a two-phase aqueous system.

[0047] Polyvinyl alcohol molecules contain a large number of hydroxyl groups, and chitosan molecules contain hydroxyl groups and amino groups. The two can interact with each other through hydrogen bonds. Under certain conditions, this hydrogen bonding will cause the polymer molecules to aggregate to form different phase regions, thus leading to phase separation. In addition, chitosan will be protonated in acidic solutions and carry a positive charge, and some hydroxyl groups in polyvinyl alcohol molecules may be ionized and carry a negative charge. The mutual attraction between positive and negative charges also plays a certain role in phase separation.

[0048] In acidic solution, the amino groups on the chitosan molecular chain will be protonated and carry a positive charge; acidic inorganic salts will ionize into anions and cations in the solution. Anions can interact with protonated chitosan through electrostatic attraction, while cations will form a certain ionic atmosphere in the solution, affecting the charge balance and intermolecular forces of the system. When a certain concentration and conditions are reached, it may cause the system to phase separate and form a double aqueous phase.

[0049] By adding the enzyme inactivator into a three-phase system composed of polyvinyl alcohol, acidic inorganic salt, chitosan and water, the effective ingredients of Sophora japonica seeds (such as rutin, etc.) can be selectively transferred to the polyvinyl alcohol phase and / or chitosan phase enrichment phase in the three-phase system, and the impurities remain in the acidic inorganic salt phase, so the extraction efficiency is high and the purification effect is better.

[0050] Rutin belongs to vitamin P, which can reduce the permeability and fragility of capillaries, and can maintain and restore the normal elasticity of capillaries.

[0051] In some embodiments, the composite dietary composition includes the following components in parts by weight: 30-40 parts of freeze-dried fruit and vegetable composite powder, 1-3 parts of European blueberry concentrated powder, 4-7 parts of locust bean powder, 0.1-5 parts of (3R, 3'R)-dihydroxy-β-carotene, 0.1-0.5 parts of lutein microcapsule powder, 30-40 parts of matcha powder, 0.01-2 parts of fermented ginger powder and 2-7 parts of complex vitamins.

[0052] The freeze-dried fruit and vegetable composite powder includes at least one of kale powder, hawthorn powder, pomegranate powder, black currant powder, acerola powder, sea buckthorn fruit powder, watermelon powder, carrot powder, tomato powder, spinach powder, barley leaf powder, celery powder or bitter melon powder.

[0053] Optionally, the freeze-dried fruit and vegetable compound powder includes kale powder, hawthorn powder, pomegranate powder, black currant powder, acerola powder, sea buckthorn fruit powder, watermelon powder, carrot powder, tomato powder, spinach powder, barley leaf powder, celery powder and bitter melon powder.

[0054] Among them, kale powder is rich in anthocyanins and dietary fiber. Hawthorn powder is rich in brass and organic acids. Pomegranate powder is rich in ellagic acid and organic acids. Blackcurrant powder is rich in flavonoids, vitamin C and anthocyanins. Acerola powder is rich in vitamin C and B vitamins. Sea buckthorn fruit powder is rich in vitamin C, flavonoids and polyphenols. Watermelon powder is rich in lycopene. Carrot powder is rich in beta-carotene. Tomato powder is rich in lycopene. Spinach powder is rich in nitrates and potassium. Barley leaf powder is rich in protein and dietary fiber. Celery powder is rich in apigenin and nitrates. Bitter melon powder is rich in bitter melon polypeptides.

[0055] In some embodiments, the freeze-dried fruit and vegetable composite powder can be prepared by a vacuum freeze-drying process (temperature is -30°C), which can retain the nutrients such as vitamins, minerals, proteins and polyphenols in the raw materials to the greatest extent; the color is close to the original color of fruits and vegetables.

[0056] The vacuum freeze-drying process can also be called the freeze-drying process. Under high vacuum conditions, the sublimation principle is used to remove the moisture in the pre-frozen material by directly sublimating the moisture into water vapor in a solid state without melting the ice.

[0057] The advantages of using vacuum freeze drying to prepare freeze-dried fruit and vegetable composite powder include: (1) Since the water is directly sublimated in a solid state, the color, texture and shape of the product remain basically unchanged, while the nutrients such as vitamins, minerals, proteins and polyphenols in the food raw materials can be retained to the greatest extent. (2) Since the drying process is carried out under low temperature and vacuum conditions, the activity of some heat-sensitive compounds and easily oxidized ingredients will not be affected.

[0058] In some embodiments, the vitamin complex includes at least three of vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B7, vitamin B12, vitamin C, vitamin A, vitamin D, and vitamin E.

[0059] Optionally, the multivitamin includes vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B7, vitamin B12, vitamin C, vitamin A, vitamin D and vitamin E.

[0060] In some embodiments, the freeze-dried fruit and vegetable composite powder is selected from kale powder, hawthorn powder, pomegranate powder, black currant powder, acerola powder, sea buckthorn fruit powder, watermelon powder, carrot powder, tomato powder, spinach powder, barley leaf powder, celery powder and bitter melon powder; the composite vitamin is selected from vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B7, vitamin B12, vitamin C, vitamin A, vitamin D and vitamin E. The compound use of 13 kinds of fruit and vegetable essences and 12 kinds of vitamins can promote the body's energy metabolism.

[0061] In some embodiments, the composite dietary composition for improving microcirculation includes 30-40 parts of freeze-dried fruit and vegetable composite powder, 1-3 parts of European blueberry concentrated powder, 4-7 parts of sophora japonica rice powder, 0.1-5 parts of (3R, 3'R)-dihydroxy-β-carotene, 0.1-0.5 parts of lutein microcapsule powder, 30-40 parts of matcha powder, 0.01-2 parts of fermented ginger powder and 2-7 parts of complex vitamins, 0.1-0.5 parts of sorbitol, 0.2-0.5 parts of microcrystalline cellulose, 0.5-1 parts of β-carotene and 0.01-0.5 parts of magnesium stearate. Among them, freeze-dried fruit and vegetable compound powder includes kale powder, hawthorn powder, pomegranate powder, black currant powder, acerola powder, sea buckthorn fruit powder, watermelon powder, carrot powder, tomato powder, spinach powder, barley leaf powder, celery powder and bitter melon powder; compound vitamins include vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B7, vitamin B12, vitamin C, vitamin A, vitamin D and vitamin E.

[0062] Among them, European blueberry concentrated powder is rich in anthocyanins, which can stabilize the microvessels in the eyes and enhance microcirculation. Anthocyanins are also a strong antioxidant that can reduce the damage of free radicals to the eyes and help prevent cataracts.

[0063] Lutein and (3R,3'R)-dihydroxy-β-carotene exist together in the retina of the eye, which can help the eyes block harmful blue light and protect the macula of the retina from damage. According to relevant studies, increasing the intake of (3R,3'R)-dihydroxy-β-carotene and lutein can reduce the risk of cataracts.

[0064] Vitamin A is an important raw material for the photosensitive substances in the eyes. Vitamin A deficiency can cause the corneal epithelial cells to fall off, thicken and keratinize, making the originally clear and transparent cornea become blurry like frosted glass, and even cause night blindness, cataracts and other eye diseases.

[0065] Vitamin E has strong antioxidant properties, which can reduce free radicals in the eye and delay eye aging.

[0066] Vitamin C has antioxidant effects, avoids light-induced oxidation reactions, prevents damage to the retina by ultraviolet rays, prevents lens aging, and increases the toughness of the eye's microvessels; it can help prevent cataracts, prevent lens clouding, and improve symptoms of blurred vision and double vision.

[0067] The composite dietary composition for improving microcirculation in the embodiment of the present application is specially supplemented with nutrients required for ocular microcirculation: European blueberry concentrated powder, lutein, (3R, 3'R)-dihydroxy-β-carotene, β-carotene, vitamin A, vitamin E and vitamin C. The nutrients coordinate with each other and work together to adjust microcirculation, promote capillary softening and promote ocular microcirculation.

[0068] The composite dietary composition for improving microcirculation in the embodiment of the present application can assist in dilating blood vessels and promoting blood flow by adding fermented ginger powder and freeze-dried fruit and vegetable composite powder, and the fermented ginger powder also has the effect of inhibiting platelet aggregation and preventing thrombosis; by adding sophora japonica powder, fermented ginger powder and vitamin D, it can assist in repairing vascular endothelium; by adding sophora japonica powder, vitamin E and vitamin C, it can assist in repairing capillary elasticity; by adding European blueberry concentrated powder, vitamin C, vitamin E, matcha powder, lutein microcapsule powder, (3R, 3'R)-dihydroxy-β-carotene and β-carotene, it can play an antioxidant role and protect microvessels; by adding B vitamins, it can promote cell metabolism.

[0069] In a second aspect, the present application also provides a method for preparing the composite dietary composition for improving microcirculation according to the first aspect, comprising: Freeze-dried fruit and vegetable composite powder, European blueberry concentrated powder, Sophora japonica rice powder, (3R, 3'R)-dihydroxy-β-carotene, lutein microcapsule powder, matcha powder, fermented ginger powder and complex vitamins are mixed with a tablet flow aid, and tablets are formed to obtain the product.

[0070] In some embodiments, the tablet glidant includes magnesium stearate. Magnesium stearate has good fluidity and compressibility, and is used as a glidant in direct tableting to facilitate tableting.

[0071] Optionally, the mass ratio of the tablet glidant to the total amount of raw materials of the composite dietary composition for improving microcirculation is 0.1% to 0.5%, for example, it can be 0.1%, 0.2%, 0.3%, 0.4% or 0.5%, etc.

[0072] The present application has been subjected to multiple tests, and part of the test results are now cited as references to further describe the invention in detail, which is described in detail below in conjunction with specific embodiments.

[0073] Example 1 The composite dietary composition for improving microcirculation of this embodiment includes the raw material components shown in Table 1: Table 1 Among them, the above-mentioned freeze-dried fruit and vegetable composite powder includes 28 g kale powder, 30 g hawthorn powder, 32 g pomegranate powder, 15 g black currant powder, 23 g acerola powder, 35 g sea buckthorn fruit powder, 28 g watermelon powder, 36 g carrot powder, 42 g tomato powder, 37 g spinach powder, 20 g barley leaf powder, 20.5 g celery powder and 20 g bitter melon powder.

[0074] The above-mentioned European blueberry concentrated powder is European blueberry concentrated powder made from wild European blueberry fruits imported from Sweden.

[0075] The above-mentioned complex vitamin includes vitamin B1 2g, vitamin B2 4g, vitamin B3 1g, vitamin B52.5g, vitamin B6 4.5g, vitamin B7 8g, vitamin B12 2.5g, vitamin C 6g, vitamin A 8g, vitamin D6.5g and vitamin E 5g.

[0076] The above-mentioned sophora japonica powder is prepared by the following steps: The dried Sophora japonica rice is crushed and then subjected to supercritical carbon dioxide enzyme inactivation treatment to obtain an enzyme inactivation product; the enzyme inactivation product is added to an extraction solvent for extraction and separation to obtain an organic phase containing effective components of Sophora japonica rice; wherein the extraction solvent comprises the following components in parts by weight: 10 parts of polyvinyl alcohol, 5 parts of ammonium sulfate (acidic inorganic salt), 70 parts of water and 3 parts of chitosan; the solid-liquid ratio of the enzyme inactivation Sophora japonica rice to the extraction solvent is 1:6; the extraction time is 10 minutes, the extraction temperature is 35°C, and the pH value is 4; The organic phase is concentrated under reduced pressure to precipitate crystals to obtain Sophora japonica flour.

[0077] The above-mentioned lutein microcapsule powder is prepared by the following steps: S1. Prepare the ingredients according to the following formula: lutein 100 mg, maltooligosaccharide 500 mg, octenylsuccinate monoarabic gum 100 mg, vitamin E 50 mg, silicon dioxide 60 mg, ascorbyl palmitate 10 mg.

[0078] S2, dissolving the composite emulsifier in water to prepare a first solution, then adding octenyl succinic acid monoarabic gum ester to the first solution and mixing, to obtain a second solution; the composite emulsifier is a composition of mealworm protein, oligomaltose and Tween in a mass ratio of 0.1:1:0.5. The mass ratio of octenyl succinic acid monoarabic gum ester to the composite emulsifier is 1:0.5.

[0079] S3. Add ascorbyl palmitate to oil and heat to dissolve to obtain a third solution. Add lutein, vitamin E and silicon dioxide to the third solution, and disperse and homogenize at a temperature of 70° C. and a rotation speed of 10,000 r / min to obtain a fourth solution.

[0080] S4, splitting the second solution into a first aqueous phase and a second aqueous phase, wherein the weight ratio of the first aqueous phase to the second aqueous phase is 2:8; splitting the fourth solution into a first oil phase and a second oil phase, wherein the weight ratio of the first oil phase to the second oil phase is 2:8.

[0081] S5. Add the first aqueous phase to the first oil phase at a rate of 1% of the total mass of the first system per minute, control the temperature to 25°C, the pH value to 4, and perform shear emulsification at a shear speed of 5000 r / min to obtain a first emulsion, wherein the total mass of the first system refers to the sum of the mass of the first aqueous phase and the mass of the first oil phase.

[0082] S6. Add the second aqueous phase to the first emulsion at a rate of 3% of the total mass of the second system per minute, control the temperature to 25°C, the pH value to 4, and perform shear emulsification at a shear speed of 10,000 r / min to obtain a second emulsion, wherein the total mass of the second system refers to the sum of the mass of the second aqueous phase and the mass of the first emulsion.

[0083] S7. Add the second oil phase to the second emulsion at a rate of 2% of the total mass of the third system per minute, control the temperature to 25°C and the pH value to 4, and perform shear emulsification at a shear speed of 25,000 r / min to obtain a third emulsion, wherein the total mass of the third system refers to the sum of the mass of the second oil phase and the mass of the second emulsion.

[0084] S8. High-pressure homogenization and spray drying of the third emulsion to obtain lutein microcapsule powder, wherein the homogenization pressure is 120 MPa.

[0085] The fermented ginger powder is prepared by the following steps: Step 1: Wash, crush, squeeze and filter the ginger to obtain ginger juice; Step 2: putting the ginger juice into a stainless steel container, sealing it, putting it into an oven, and pasteurizing it at 80°C. After that, taking it out and cooling it to room temperature, inoculating Aspergillus oryzae, and fermenting it at 30°C for at least 48 hours to obtain ginger fermentation product; Step 3: Dry the fermented ginger to obtain fermented ginger powder.

[0086] The steps for preparing the composite dietary composition for improving microcirculation in the embodiment of the present application are as follows: Weigh each raw material according to the formula shown in Table 1 and set aside; 366.5 g of freeze-dried fruit and vegetable composite powder, 12 g of European blueberry concentrated powder, 43.5 g of sophora japonica rice powder, 1.25 g of (3R, 3'R)-dihydroxy-β-carotene, 3.25 g of lutein microcapsule powder, 312.5 g of matcha powder, 10.5 g of fermented ginger powder and 50 g of complex vitamins are mixed with 2.4 g of magnesium stearate, and tablets are formed to obtain tablets of a composite dietary composition for improving microcirculation.

[0087] Example 2 The composite dietary composition for improving microcirculation of this embodiment includes the raw material components shown in Table 2: Table 2 The freeze-dried fruit and vegetable composite powder includes 20 g kale powder, 25 g hawthorn powder, 22 g pomegranate powder, 15 g black currant powder, 20 g acerola powder, 25 g sea buckthorn fruit powder, 25 g watermelon powder, 30 g carrot powder, 22 g tomato powder, 35.5 g spinach powder, 20 g barley leaf powder, 20.5 g celery powder and 20 g bitter melon powder.

[0088] The above-mentioned European blueberry concentrated powder is European blueberry concentrated powder made from wild European blueberry fruits imported from Sweden.

[0089] The above-mentioned complex vitamin includes 5g of vitamin B1, 5g of vitamin B2, 1g of vitamin B3, 3g of vitamin B5, 5g of vitamin B6, 8g of vitamin B7, 5g of vitamin B12, 8g of vitamin C, 10g of vitamin A, 10g of vitamin D and 9g of vitamin E.

[0090] The preparation method of the sophora japonica powder of the present embodiment is the same as the preparation method of the sophora japonica powder of the above embodiment 1.

[0091] The above-mentioned lutein microcapsule powder is prepared by the following steps: S1. Prepare the ingredients according to the following formula: lutein 100 mg, maltooligosaccharide 500 mg, octenylsuccinate monoarabic gum 100 mg, vitamin E 50 mg, silicon dioxide 60 mg, ascorbyl palmitate 10 mg.

[0092] S2, dissolving the composite emulsifier in water to prepare a first solution, then adding octenyl succinic acid monoarabic gum ester to the first solution and mixing, to obtain a second solution; the composite emulsifier is a composition of yellow mealworm protein, oligomaltose and Tween in a mass ratio of 0.1:1:0.5. The mass ratio of octenyl succinic acid monoarabic gum ester to the composite emulsifier is 1:0.1.

[0093] S3, adding ascorbyl palmitate to oil and heating to dissolve to obtain a third solution, adding lutein, vitamin E and silicon dioxide to the third solution, and dispersing and homogenizing at a temperature of 60° C. and a rotation speed of 15000 r / min to obtain a fourth solution; S4, splitting the second solution into a first aqueous phase and a second aqueous phase, wherein the weight ratio of the first aqueous phase to the second aqueous phase is 2:8; splitting the fourth solution into a first oil phase and a second oil phase, wherein the weight ratio of the first oil phase to the second oil phase is 2:8.

[0094] S5. Add the first aqueous phase to the first oil phase at a rate of 2% of the total mass of the first system per minute, control the temperature to 35°C, the pH value to 5, and perform shear emulsification at a shear speed of 6000 r / min to obtain a first emulsion, wherein the total mass of the first system refers to the sum of the mass of the first aqueous phase and the mass of the first oil phase.

[0095] S6. Add the second aqueous phase to the first emulsion at a rate of 5% of the total mass of the second system per minute, control the temperature to 35°C, the pH value to 5, and perform shear emulsification at a shear speed of 20,000 r / min to obtain a second emulsion, wherein the total mass of the second system refers to the sum of the mass of the second aqueous phase and the mass of the first emulsion.

[0096] S7. Add the second oil phase to the second emulsion at a rate of 3% of the total mass of the third system per minute, control the temperature to 35°C, the pH value to 5, and perform shear emulsification at a shear speed of 25,000 r / min to obtain a third emulsion, wherein the total mass of the third system refers to the sum of the mass of the second oil phase and the mass of the second emulsion.

[0097] S8. High-pressure homogenization and spray drying of the emulsion are performed to obtain lutein microcapsule powder, wherein the homogenization pressure is 80 MPa.

[0098] The preparation method of the fermented ginger powder of this embodiment is the same as the preparation method of the fermented ginger powder of the above-mentioned embodiment 1.

[0099] The steps for preparing the composite dietary composition for improving microcirculation in the embodiment of the present application are as follows: Weigh each raw material according to the formula shown in Table 2 and set aside; 300 g of freeze-dried fruit and vegetable composite powder, 120 g of European blueberry concentrated powder, 50 g of sophora japonica powder, 1.25 g of (3R, 3'R)-dihydroxy-β-carotene, 3.5 g of lutein microcapsule powder, 312.5 g of matcha powder, 11 g of fermented ginger powder and 69 g of complex vitamins are mixed with 2.0 g of magnesium stearate, and tablets are formed to obtain tablets of a composite dietary composition for improving microcirculation.

[0100] Example 3 The composite dietary composition for improving microcirculation of this embodiment includes the raw material components shown in Table 3: Table 3 Among them, the above-mentioned freeze-dried fruit and vegetable composite powder includes 38 g kale powder, 30 g hawthorn powder, 22 g pomegranate powder, 25 g black currant powder, 30 g acerola powder, 35 g sea buckthorn fruit powder, 28 g watermelon powder, 35 g carrot powder, 35 g tomato powder, 42 g spinach powder, 30 g barley leaf powder, 26 g celery powder and 24 g bitter melon powder.

[0101] The above-mentioned complex vitamin includes 2.5g of vitamin B1, 1.8g of vitamin B2, 1.5g of vitamin B3, 1.65g of vitamin B5, 1.45g of vitamin B6, 2.8g of vitamin B7, 1.6g of vitamin B12, 1.65g of vitamin C, 2.5g of vitamin A, 4g of vitamin D and 3.55g of vitamin E.

[0102] The above-mentioned lutein microcapsule powder is prepared by the following steps: S1. Prepare the ingredients according to the following formula: lutein 200 mg, maltooligosaccharide 1000 mg, octenylsuccinate monoarabic gum 500 mg, vitamin E 70 mg, silicon dioxide 100 mg, ascorbyl palmitate 20 mg.

[0103] S2, dissolving the composite emulsifier in water to prepare a first solution, then adding octenyl succinic acid monoarabic gum ester to the first solution and mixing, to obtain a second solution; the composite emulsifier is a composition of yellow mealworm protein, oligomaltose and Tween in a mass ratio of 0.1:1:0.5. The mass ratio of octenyl succinic acid monoarabic gum ester to the composite emulsifier is 1:0.3.

[0104] S3, adding ascorbyl palmitate to oil and heating to dissolve to obtain a third solution, adding lutein, vitamin E and silicon dioxide to the third solution, and dispersing and homogenizing at a temperature of 70° C. and a rotation speed of 10,000 r / min to obtain a fourth solution; S4, splitting the second solution into a first aqueous phase and a second aqueous phase, wherein the weight ratio of the first aqueous phase to the second aqueous phase is 2:8; splitting the fourth solution into a first oil phase and a second oil phase, wherein the weight ratio of the first oil phase to the second oil phase is 2:8.

[0105] S5. Add the first aqueous phase to the first oil phase at a rate of 2% of the total mass of the first system per minute, control the temperature to 45°C, the pH value to 7, and perform shear emulsification at a shear speed of 10,000 r / min to obtain a first emulsion, wherein the total mass of the first system refers to the sum of the mass of the first aqueous phase and the mass of the first oil phase.

[0106] S6. Add the second aqueous phase to the first emulsion at a rate of 4% of the total mass of the second system per minute, control the temperature to 45°C, the pH value to 7, and perform shear emulsification at a shear speed of 20,000 r / min to obtain a second emulsion, wherein the total mass of the second system refers to the sum of the mass of the second aqueous phase and the mass of the first emulsion.

[0107] S7. Add the second oil phase to the second emulsion at a rate of 3% of the total mass of the third system per minute, control the temperature to 45°C and the pH value to 7, and perform shear emulsification at a shear speed of 25,000 r / min to obtain a third emulsion, wherein the total mass of the third system refers to the sum of the mass of the second oil phase and the mass of the second emulsion.

[0108] S8. High-pressure homogenization and spray drying of the emulsion are performed to obtain lutein microcapsule powder, wherein the homogenization pressure is 120 MPa.

[0109] The raw materials of the European blueberry concentrated powder, the sophora japonica rice powder, and the fermented ginger powder of this embodiment are the same as the raw materials of the European blueberry concentrated powder, the sophora japonica rice powder, and the fermented ginger powder of Example 1.

[0110] The steps for preparing the composite dietary composition for improving microcirculation in the embodiment of the present application are as follows: Weigh each raw material according to the formula shown in Table 3 and set aside; 400 g of freeze-dried fruit and vegetable composite powder, 200 g of European blueberry concentrated powder, 70 g of sophora japonica powder, 5 g of (3R, 3'R)-dihydroxy-β-carotene, 4 g of lutein microcapsule powder, 200 g of matcha powder, 15 g of fermented ginger powder and 25 g of complex vitamins were mixed with 2.4 g of magnesium stearate, and tablets were formed to obtain a composite dietary composition tablet for improving microcirculation with a tablet weight of 0.8 g.

[0111] Comparative Example 1 The composite dietary composition for improving microcirculation of this comparative example comprises the raw material components shown in Table 4: Table 4 The raw materials in Table 4 were used to prepare the composite dietary composition tablets for improving microcirculation of this comparative example by referring to the preparation method of Example 1.

[0112] Comparative Example 2 The composite dietary composition for improving microcirculation of this comparative example comprises the raw material components shown in Table 5: Table 5 The raw materials in Table 5 were used to prepare the composite dietary composition tablets for improving microcirculation of this comparative example by referring to the preparation method of Example 1.

[0113] Comparative Example 3 The composite dietary composition for improving microcirculation of this comparative example comprises the raw material components shown in Table 6: Table 6 The composite dietary composition tablets for improving microcirculation of this comparative example were prepared by using the raw materials in Table 6 and referring to the preparation method of Example 1.

[0114] Comparative Example 4 The composite dietary composition for improving microcirculation of this comparative example comprises the raw material components shown in Table 7: Table 7 The composite dietary composition tablets for improving microcirculation of this comparative example were prepared by using the raw materials in Table 7 and referring to the preparation method of Example 1.

[0115] Comparative Example 5 The composite dietary composition for improving microcirculation of this comparative example comprises the raw material components shown in Table 8: Table 8 The raw materials in Table 8 were used to prepare the composite dietary composition tablets for improving microcirculation of this comparative example by referring to the preparation method of Example 1.

[0116] Wherein, the preparation steps of common ginger powder are as follows: Step 1: Wash, crush, squeeze and filter the ginger to obtain ginger juice; Step 2: Dry the ginger juice obtained in step 1 to obtain common ginger powder.

[0117] Comparative Example 6 The composite dietary composition of this comparative example is different from the composite dietary composition of Example 1 in that the lutein microcapsule powder is composed of the following components in parts by weight: lutein 100 mg, maltooligosaccharide 500 mg, octenylsuccinic acid monoarabic gum ester 100 mg, vitamin E 50 mg, and ascorbyl palmitate 10 mg.

[0118] Comparative Example 7 The composite dietary composition of this comparative example is different from the composite dietary composition of Example 1 above only in that the lutein microcapsule powder is composed of the following components in parts by weight: lutein 100 mg, maltooligosaccharide 500 mg, octenylsuccinic acid monoarabic gum ester 100 mg, silicon dioxide 60 mg, and ascorbic acid palmitate 10 mg.

[0119] Comparative Example 8 The composite dietary composition of this comparative example is different from the composite dietary composition of Example 1 in that the lutein microcapsule powder is composed of the following components in parts by weight: lutein 100 mg, maltooligosaccharide 500 mg, octenylsuccinic acid monoarabic gum ester 100 mg, vitamin E 50 mg, and silicon dioxide 60 mg.

[0120] Comparative Example 9 The composite dietary composition of this comparative example differs from the composite dietary composition of Example 1 only in that: lutein ester 100 mg, maltooligosaccharide 500 mg, octenylsuccinate monoarabic gum ester 100 mg, vitamin E 50 mg, silicon dioxide 60 mg, ascorbic acid palmitate 10 mg.

[0121] In order to further illustrate the improvement effect of the composite dietary composition for improving microcirculation prepared in the examples of the present application on ocular microcirculation, the following performance tests were performed on the tablets of the composite dietary composition for improving microcirculation prepared in Examples 1 to 3 and Comparative Examples 1 to 9 of the present application.

[0122] 1) Test object: A total of 80 subjects aged 20-45 years with symptoms of conjunctival arteriovenous constriction, cystic dilatation and spiral curvature of the conjunctiva, and changes in microvessels such as exudation, bleeding, and hemosiderin deposition were selected. The male-to-female ratio was approximately 1:1. They were randomly divided into 3 groups (Group 1 to Group 12), with 10 subjects in each group. The male-to-female ratio of each group was approximately 1:1.

[0123] 2) Testing instruments and methods: The microcirculation and microvessels of the bulbar conjunctiva were detected using an integrated microcirculation detector (developed by Shanghai Laser Technology Research Institute). During the test, the subject sat down and placed his head on the microcirculation support to observe the changes in the microcirculation and microvessels of the bulbar conjunctiva on the temporal side of the eye. The score analysis was performed using Tian's comprehensive quantitative method of microcirculation.

[0124] During fundus angiography, the pupil is first dilated, and then 3 ml (0.6 g) of sodium fluorescein is injected into the subject's forearm vein. The fundus blood vessels are immediately observed and photographed continuously using a TOPCON slit lamp microscope, and the results are analyzed.

[0125] 3) Usage and dosage: The 10 subjects in Group 1 took the composite dietary composition tablets for improving microcirculation prepared in Example 1 of the present application, 2 tablets each time, twice a day (once in the morning and once in the evening), which can be taken with warm water or chewed orally according to personal preference, for 10 days per course of treatment.

[0126] The 10 subjects in Group 2 took the composite dietary composition tablets for improving microcirculation prepared in Example 2 of the present application, 2 tablets each time, twice a day (once in the morning and once in the evening), which can be taken with warm water or chewed orally according to personal preference, for 10 days per course of treatment.

[0127] The 10 subjects in Group 3 took the composite dietary composition tablets for improving microcirculation prepared in Example 3 of the present application, 2 tablets each time, twice a day (once in the morning and once in the evening), which can be taken with warm water or chewed orally according to personal preference, for 10 days per course of treatment.

[0128] The 10 subjects in Group 4 took the microcirculation improving composite dietary composition tablets prepared in Comparative Example 1, 2 tablets each time, twice a day (once in the morning and once in the evening), which could be taken with warm water or chewed orally according to personal preference, for 10 days per course of treatment.

[0129] The 10 subjects in Group 5 took the microcirculation improving composite dietary composition tablets prepared in Comparative Example 2, 2 tablets each time, twice a day (once in the morning and once in the evening), which could be taken with warm water or chewed orally according to personal preference, for 10 days per course of treatment.

[0130] The 10 subjects in Group 6 took the microcirculation improving composite dietary composition tablets prepared in Comparative Example 3, 2 tablets each time, twice a day (once in the morning and once in the evening), which can be taken with warm water or chewed orally according to personal preference, for 10 days per course of treatment.

[0131] The 10 subjects in Group 7 took the microcirculation improving composite dietary composition tablets prepared in Comparative Example 4, 2 tablets each time, twice a day (once in the morning and once in the evening), which can be taken with warm water or chewed orally according to personal preference, for 10 days per course of treatment.

[0132] The 10 subjects in Group 8 took the microcirculation improving composite dietary composition tablets prepared in Comparative Example 5, 2 tablets each time, twice a day (once in the morning and once in the evening), which could be taken with warm water or chewed orally according to personal preference, for 10 days per course of treatment.

[0133] The 10 subjects in Group 9 took the composite dietary composition tablets for improving microcirculation prepared in Comparative Example 6, 2 tablets each time, twice a day (once in the morning and once in the evening), which could be taken with warm water or chewed orally according to personal preference, for 10 days per course of treatment.

[0134] The 10 subjects in Group 10 took the microcirculation improving composite dietary composition tablets prepared in Comparative Example 7, 2 tablets each time, twice a day (once in the morning and once in the evening), which can be taken with warm water or chewed orally according to personal preference, for 10 days per course of treatment.

[0135] The 10 subjects in Group 11 took the microcirculation improving composite dietary composition tablets prepared in Comparative Example 8, 2 tablets each time, twice a day (once in the morning and once in the evening), which could be taken with warm water or chewed orally according to personal preference, for 10 days per course of treatment.

[0136] The 10 subjects in Group 12 took the microcirculation improving composite dietary composition tablets prepared in Comparative Example 9, 2 tablets each time, twice a day (once in the morning and once in the evening), which could be taken with warm water or chewed orally according to personal preference, for 10 days per course of treatment.

[0137] 4) Statistical analysis: After 3 courses of treatment, the results of the conjunctival microcirculation test of the subjects in Group 1 to Group 12 were comprehensively and quantitatively analyzed. The results are shown in Table 9 below.

[0138] Table 9 Changes in the integral values ​​of conjunctival microcirculation in the three groups of subjects before and after treatment (x±s) Note: In Table 9, * indicates p < 0.01 compared with before treatment, and # indicates p < 0.05 compared with before treatment.

[0139] As can be seen from Table 9 above, after the subjects in Group 1 to Group 3 were treated with the composite dietary composition tablets for improving microcirculation provided by Examples 1 to 3 of the present application for three courses, the total score of the conjunctiva was reduced to varying degrees, and the conjunctival microcirculation was significantly improved, which shows that the composite dietary composition tablets for improving microcirculation provided by the examples of the present application have good effects on adjusting microcirculation, promoting capillary softening, and promoting ocular microcirculation.

[0140] After three courses of treatment with the composite dietary composition tablets for improving microcirculation provided by Comparative Examples 1 to 5, the subjects in Groups 4 to 8 had different degrees of reduction in the total score of the bulbar conjunctiva, and the bulbar conjunctival microcirculation was also well improved, but the improvement effect was slightly worse than that of Groups 1 to 3. It can be seen that the simultaneous addition of (3R, 3'R)-dihydroxy-β-carotene, lutein microcapsule powder, sophora japonica powder and fermented ginger powder to the product has a synergistic effect, thereby achieving a better effect of adjusting microcirculation, promoting capillary softening and promoting eye microcirculation.

[0141] After three courses of treatment with the composite dietary composition tablets for improving microcirculation provided by Comparative Examples 6 to 8, the subjects in Groups 9 to 11 had different degrees of reduction in the total score of the bulbar conjunctiva, and the bulbar conjunctival microcirculation was also well improved, but the improvement effect was slightly worse than that of Groups 1 to 3. It can be seen that the simultaneous addition of ascorbyl palmitate, vitamin E and silicon dioxide in the preparation of lutein microcapsule powder has a synergistic effect, which can better delay the loss of part or all of the activity of lutein due to oxidation, improve the uniform dispersion effect of lutein, and improve its bioavailability, thereby achieving better effects of adjusting microcirculation, promoting capillary softening, and promoting ocular microcirculation.

[0142] After three courses of treatment with the composite dietary composition tablets for improving microcirculation provided in Comparative Example 9, the total score of the bulbar conjunctiva was not significantly different before and after treatment, and the improvement effect of the bulbar conjunctiva microcirculation was not obvious. It can be seen that compared with the use of lutein ester microcapsule powder, the use of lutein microcapsule powder in the embodiment of the present application can achieve better effects of adjusting microcirculation, promoting capillary softening, and promoting eye microcirculation.

[0143] In addition, the present application has found through a large number of experiments that the stability and sustained release of the lutein microcapsule powder provided in the examples of the present application will be significantly affected by changes in the preparation process conditions of the lutein microcapsule powder, as shown in the following experiments.

[0144] Experiment 1: Exploring the effects of different wall materials on the quality of lutein microcapsule powder products Each group of tests adopted the same experimental conditions as the lutein microcapsule powder of Example 1, except that the wall material (octenyl succinate monoarabic gum ester: composite emulsifier = 1:0.5 (mass ratio)) in Example 1 was replaced with various materials in Tables 10 to 12 below of equal mass, and the following performance index tests were performed on the prepared lutein microcapsule powders. The test results are shown in Tables 10 to 12: Among them, the test method / evaluation method of each performance indicator is as follows: 1) Surface oil content: The method is carried out according to the method specified in Appendix A of SC / T 3505-2006 "Fish Oil Microcapsules".

[0145] 2) In vitro dissolution: The in vitro dissolution test was carried out according to the dissolution and release rate determination method of General Chapter 0931 of the 2020 edition of the Chinese Pharmacopoeia (Volume IV). The artificial intestinal fluid containing trypsin at pH 6.8 and artificial gastric fluid containing pepsin were used as dissolution media, and the dissolution of lutein microcapsule powder in the human gastrointestinal tract was simulated by testing the solubility of lutein.

[0146] Preparation method of artificial gastric juice: Take 16.4 ml of dilute hydrochloric acid, add about 800 ml of water and 10 g of pepsin (the amount of enzyme is adjusted according to the enzyme activity), shake well, and then dilute with water to make a volume of 1000 ml.

[0147] Preparation method of artificial intestinal fluid: Take 6.8 g of potassium dihydrogen phosphate, add 500 ml of water to dissolve, and adjust the pH value to 6.8 with 0.1 mol / L sodium hydroxide solution; take 10 g of trypsin (the amount of enzyme is adjusted according to the enzyme activity) and 2 g of pancreatic lipase, add appropriate amount of water to dissolve, mix the two liquids, and dilute with water to make the volume 1000 ml.

[0148] Dissolution content determination method: 120 mg of lutein microcapsule powder was accurately weighed, and the paddle method was used in a 37 ℃ water bath at 50 r / min. The solution was stirred and dissolved in 500 ml of artificial gastric juice and artificial intestinal juice respectively. After 30 min, 1 h, 2 h, 4 h and 8 h, 5 ml of the solution was taken and quickly passed through a 0.45 μm water microporous filter membrane to determine the cumulative dissolution of the solution. Among them, the cumulative dissolution = (the total amount of lutein dissolved / the amount of lutein input) × 100%.

[0149] 3) Fluidity evaluation method: Measure the powder repose angle according to GB11986-89 "Measurement of the repose angle of surfactant powders and particles". If the repose angle is less than 33°, the powder fluidity is "good"; if the repose angle is ≥ 40°, the powder fluidity is "average"; if the repose angle is greater than 40°, the powder fluidity is "poor".

[0150] Table 10 Effect of different wall materials on the encapsulation efficiency of lutein microcapsule powder Surface oil content (surface lutein content) is an important indicator for evaluating the microencapsulation efficiency of a product. The lower the surface lutein content, the better the effect of encapsulation of the core material lutein.

[0151] As shown in Table 10, the composite of octenyl succinic acid monoarabic gum ester and composite emulsifier in a mass ratio of 1:0.1~0.5 as wall material has a good embedding effect on the core material component (lutein), and the surface oil content is only 0.1~0.18%. The embedding effect of octenyl succinic acid monoarabic gum ester and composite emulsifier in a mass ratio of 0.5:1 as wall material is slightly worse than the embedding effect of octenyl succinic acid monoarabic gum ester and composite emulsifier in a mass ratio of 1:0.1~0.5 as wall material. The embedding effect of using octenyl succinic acid monoarabic gum ester alone, or using gum arabic, or using gum arabic and composite emulsifier in a mass ratio of 1:0.5 as wall material is poor. It can be seen that the embodiment of the present application can improve the embedding effect of the product, reduce the surface oil content of the product, and help improve the quality and stability of the product by introducing a composite emulsifier into octenyl succinic acid monoarabic gum ester and controlling the mass ratio of octenyl succinic acid monoarabic gum ester to the composite emulsifier to be 1:0.1~0.5.

[0152] Table 11 Dissolution of lutein microcapsules made of different wall materials in gastric juice at different times As shown in Table 11, the lutein microcapsule powder prepared by using the compound of octenyl succinic acid monoarabic gum ester and composite emulsifier in a mass ratio of 1:0.5 as the wall material has a sustained release time of up to 8 hours and a long onset time. The dissolution amount at each time point increases evenly, the prepared lutein microcapsule powder is released slowly, the lutein put in can be released 100%, and it still maintains a high therapeutic plasma level after long-term use, and the release is slow, which greatly improves the bioavailability of lutein. The sustained-release effect of the lutein microcapsule powder prepared by using octenyl succinic acid monoarabic gum ester and a composite emulsifier in a mass ratio of 0.5: 1 as a wall material, or using octenyl succinic acid monoarabic gum ester alone, or using gum arabic, or using gum arabic and a composite emulsifier in a mass ratio of 1: 0.5 as a wall material, is poorer than the sustained-release effect of the lutein microcapsule powder prepared by using octenyl succinic acid monoarabic gum ester and a composite emulsifier in a mass ratio of 1: 0.5 as a wall material, and the bioavailability is relatively poor. It can be seen that the embodiment of the present application uses a composite of a specific ratio of octenyl succinic acid monoarabic gum ester and a composite emulsifier as a wall material, and the lutein microcapsule powder prepared has excellent sustained-release performance, which greatly improves the bioavailability.

[0153] In addition, the dissolution rate of lutein microcapsule powder with different wall materials in intestinal fluid at different times is similar to the dissolution rate of lutein microcapsule powder with different wall materials in gastric fluid at different times.

[0154] Table 12 Flowability of lutein microcapsule powders made from different wall materials As shown in Table 12, the lutein microcapsule powder obtained by using a composite of octenyl succinic acid monoarabic gum ester and oligomaltose in a mass ratio of 1: 0.1 to 0.5 as a wall material has good fluidity, while the lutein microcapsule powder obtained by using octenyl succinic acid monoarabic gum ester and a composite in a mass ratio of 5: 1, or using octenyl succinic acid monoarabic gum ester alone, or using gum arabic, or using gum arabic and a composite emulsifier in a mass ratio of 1: 0.5 as a wall material is generally fluid. It can be seen that the embodiment of the present application is beneficial to improve the fluidity of lutein microcapsule powder by using octenyl succinic acid monoarabic gum ester and a composite emulsifier in a mass ratio of 1: 0.1 to 0.5 as a wall material.

[0155] Experiment 2: Investigating the effect of emulsifier type on the storage stability and particle size distribution of emulsions Each group of experiments adopted the same experimental conditions as those for preparing the lutein microcapsule powder in Example 1, except that the composite emulsifier in the step of preparing the lutein microcapsule powder in Example 1 was replaced with the emulsifiers of the same mass in Table 13 below to prepare the third emulsion. The storage stability and particle size distribution of each group of the third emulsions prepared were tested, and the test results are shown in Table 13.

[0156] The test method is as follows: take the same volume of each group of the third emulsion, seal it in a transparent reagent bottle, store it at 25°C (degrees Celsius) away from light, observe and record the storage stability and particle size distribution of the third emulsion every 5 days, and continue to record for 60 days. The test results are shown in Table 13.

[0157] The particle size and distribution of the emulsion droplets of each group of the third emulsion were measured using a Winner 801 laser particle size analyzer.

[0158] Table 13 Storage stability and particle size distribution of the third emulsion prepared with different emulsifiers The storage stability of the emulsion is divided into three levels: good, fair, and poor. Good means that no stratification, flocculation, or aggregation occurs after 60 days of storage. Fair means that stratification, flocculation, or aggregation occurs during 15 to 60 days of storage. Poor means that stratification, flocculation, or aggregation occurs after 5 to 10 days of storage.

[0159] As shown in Table 13, the storage stability of the third emulsion obtained by using any one or two of yellow mealworm protein, oligomaltose or Tween as emulsifiers is average. The particle size of the third emulsion obtained by using any one of yellow mealworm protein, oligomaltose or Tween as an emulsifier is relatively large, about 170~200nm; the particle size of the third emulsion obtained by using any two of yellow mealworm protein, oligomaltose or Tween as emulsifiers is relatively small, about 140nm~150nm. The third emulsion obtained by using a composite emulsifier (a combination of yellow mealworm protein, oligomaltose and Tween) has good storage stability, and the particle size of the third emulsion is very small, about 20nm. Therefore, the compounding of yellow mealworm protein, oligomaltose and Tween can not only improve the storage stability of the emulsion, but also obtain an emulsion with a narrow particle size distribution and a smaller particle size, which is beneficial to improve the bioavailability of lutein in the human body.

[0160] Experiment 3: Investigating the effects of different pH values ​​on the storage stability and particle size distribution of emulsions Each group of experiments adopted the same experimental conditions as those for preparing lutein microcapsule powder in Example 1, except that the pH values ​​of the first emulsion, the second emulsion and the third emulsion in Example 1 were adjusted to 3, 4, 5, 6, 7 and 8, respectively. The storage stability and particle size distribution of each group of the third emulsion were tested with reference to the test method of Experiment 2, and the test results are shown in Table 14.

[0161] Table 14 Effect of different pH values ​​on storage stability and particle size distribution of emulsion It can be seen from Table 14 that when preparing the first emulsion, the second emulsion and the third emulsion, controlling the pH value between 3 and 7 can improve the storage stability of the emulsion and obtain an emulsion with a smaller particle size. When the pH value is less than 4 or greater than 7, the storage stability of the emulsion is average, and the particle size of the emulsion is larger.

[0162] Experiment 4: Investigating the effect of shear emulsification process on the storage stability and particle size distribution of emulsions Each group of experiments adopted the same experimental conditions as those for preparing lutein microcapsule powder in Example 1, except that steps S4 to S7 in Example 1 were replaced by "slowly adding the fourth solution to the second solution, controlling the temperature to 25 °C, the pH value to 4, and performing shear emulsification at a shear speed of 10000 r / min to obtain an emulsion". The storage stability and particle size distribution of the prepared emulsion were tested with reference to the test method of Experiment 2. The test results showed that the storage stability of the emulsion was average, and the particle size distribution of the emulsion particles was D 90 Around 200nm.

[0163] It can be seen that Example 1 of the present application first splits the second solution into the first aqueous phase and the second aqueous phase, splits the fourth solution into the first oil phase and the second oil phase, and then controls the order and speed of adding the aqueous phase and the oil phase as well as the shear speed in each step, so that the oil phase and the aqueous phase in the emulsion system are fully mixed during the emulsification process, thereby improving the stability of the emulsion and the uniformity of the particle size distribution, and at the same time improving the preparation efficiency of the emulsion.

[0164] Experiment 5: Investigating the influence of extraction process conditions of Sophora japonica powder on extraction effect Each group of experiments adopted the same experimental conditions as the sophora japonica powder in Example 1, except that the extraction solvent of the sophora japonica powder in Example 1 was replaced with an equal volume of various extraction solvents in Table 15 below, and the following performance index tests were performed on the prepared sophora japonica powder. The test results are shown in Table 15: Table 15 Effects of different extraction solvents on the extraction of active ingredients (in terms of rutin) from Sophora japonica seeds As shown in Table 15, the three-phase system formed by the compounding of polyvinyl alcohol, ammonium sulfate, water and chitosan can be used as the extraction solvent to effectively extract the effective component (rutin) of Sophora japonica. The extraction effect is the best, and the rutin yield can reach 312 mg / g. However, the two-phase system formed by the compounding of polyvinyl alcohol and chitosan, the two-phase system formed by the compounding of polyvinyl alcohol and ammonium sulfate, or the two-phase system formed by the compounding of ammonium sulfate and chitosan as the extraction solvent, the extraction effect of the effective components of Sophora japonica on the effective components of Sophora japonica is lower than the extraction effect of the extraction solvent prepared by polyvinyl alcohol, ammonium sulfate, water and chitosan on the effective components of Sophora japonica. It can be seen that the use of polyvinyl alcohol, ammonium sulfate and chitosan has a synergistic effect, and when polyvinyl alcohol, ammonium sulfate, water and chitosan are prepared as the extraction solvent in a mass ratio of 10:5:70:3, the effective component (rutin) of Sophora japonica can be effectively extracted, and the rutin yield is the highest. Therefore, the present application preferably uses an extraction solvent prepared by mixing polyvinyl alcohol, ammonium sulfate, water and chitosan in a mass ratio of 10:5:70:3 to extract the active ingredients of Sophora japonica seeds.

[0165] The above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit them. Although the present application has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. These modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present application, and should all be included in the protection scope of the present application.

Claims

1. A composite dietary composition for improving microcirculation, characterized in that: The components include: Freeze-dried fruit and vegetable compound powder, European blueberry concentrated powder, Sophora japonica rice powder, (3R,3'R)-dihydroxy-β-carotene, lutein microcapsule powder, matcha powder, fermented ginger powder and multivitamins; The lutein microcapsule powder comprises the following components: lutein, maltooligosaccharide, octenylsuccinate monoarabic gum ester, vitamin E, silicon dioxide and ascorbyl palmitate; The preparation steps of the lutein microcapsule powder are as follows: Dissolving the composite emulsifier in water to prepare a first solution, and then adding octenyl succinic acid monoarabic gum ester to the first solution and mixing them to obtain a second solution; Add ascorbyl palmitate to oil and fat, heat and dissolve to obtain a third solution, add lutein, vitamin E and silicon dioxide to the third solution, disperse and homogenize to obtain a fourth solution; Splitting the second solution into a first aqueous phase and a second aqueous phase; Splitting the fourth solution into a first oil phase and a second oil phase; Adding the first water phase into the first oil phase at a first speed for shear emulsification to obtain a first emulsion; adding the second aqueous phase into the first emulsion at a second speed for shear emulsification to obtain a second emulsion; Adding the second oil phase to the second emulsion at a third speed for shear emulsification to obtain a third emulsion; wherein the first speed is lower than the third speed, and the third speed is lower than the second speed; The third emulsion is subjected to high pressure homogenization and spray drying to obtain lutein microcapsule powder.

2. The composite dietary composition for improving microcirculation according to claim 1, characterized in that: The composite emulsifier is a composition of mealworm protein, maltooligosaccharide and Tween; And / or, the mass ratio of the octenyl succinic acid monoarabic gum ester to the composite emulsifier is 1:0.1~0.

5.

3. The composite dietary composition for improving microcirculation according to claim 1, characterized in that: In the steps of preparing the first emulsion, the second emulsion and the third emulsion, the temperature is controlled to be 25-45° C. and the pH value is controlled to be 4-7.

4. The composite dietary composition for improving microcirculation according to claim 1, characterized in that: Adding the first water phase to the first oil phase at a first speed for shear emulsification to obtain a first emulsion comprises: The first water phase is added to the first oil phase at a rate of 1% to 2% of the total mass of the first system per minute, and shear emulsification is performed at a shear speed of 5000 to 10000 r / min to obtain a first emulsion; wherein the total mass of the first system refers to the sum of the mass of the first water phase and the mass of the first oil phase; Adding the second aqueous phase into the first emulsion at a second speed for shear emulsification to obtain a second emulsion comprises: The second aqueous phase is added to the first emulsion at a rate of 3% to 5% of the total mass of the second system per minute, and shear emulsification is performed at a shear speed of 10000 to 25000 r / min to obtain a second emulsion; wherein the total mass of the second system refers to the sum of the mass of the second aqueous phase and the mass of the first emulsion; The second oil phase is added to the second emulsion at a third speed for shear emulsification to obtain a third emulsion, comprising: The second oil phase is added to the second emulsion at a rate of 1% to 3% of the total mass of the third system per minute, and shear emulsification is performed at a shear speed of 10,000 to 25,000 r / min to obtain a third emulsion; wherein the total mass of the third system refers to the sum of the mass of the second oil phase and the mass of the second emulsion.

5. The composite dietary composition for improving microcirculation according to claim 1, characterized in that: The lutein microcapsule powder comprises the following components in parts by weight: 10-20 parts of lutein, 1-100 parts of maltooligosaccharide, 10-50 parts of octenylsuccinate monoarabic gum ester, 5-7 parts of vitamin E, 5-10 parts of silicon dioxide and 1-2 parts of ascorbyl palmitate; The diameter of the lutein microcapsule powder is 1-100 nm, and the thickness of the wall is 0.5-150 nm.

6. The composite dietary composition for improving microcirculation according to claim 1, characterized in that: The preparation steps of the fermented ginger powder are as follows: Washing, crushing, squeezing and filtering ginger to obtain ginger juice; After sterilizing the ginger juice, a fermentation strain is added, and the ginger juice is fermented at 25-35° C. for at least 48 hours to obtain a ginger fermentation product; the fermentation strain is at least one of Candida utilis, Acetobacter or Aspergillus oryzae; and the mass ratio of the fermentation strain to the ginger juice is 1:100-150; The ginger fermented product is dried to obtain fermented ginger powder.

7. The composite dietary composition for improving microcirculation according to claim 1, characterized in that: The preparation steps of the sophora japonica powder are as follows: The dried Sophora japonica seeds are crushed and then subjected to a supercritical carbon dioxide enzyme inactivation treatment to obtain an enzyme inactivation product; The enzyme-killing substance is added to an extraction solvent for extraction and separation to obtain an organic phase containing effective ingredients of Sophora japonica seed; wherein the extraction solvent comprises the following components in parts by weight: 10-40 parts of polyvinyl alcohol, 5-40 parts of acidic inorganic salt, 70-90 parts of water and 2-10 parts of chitosan; The organic phase is concentrated under reduced pressure to precipitate crystals to obtain Sophora japonica flour.

8. The composite dietary composition for improving microcirculation according to claim 7, characterized in that: The solid-liquid ratio of the enzyme-inactivating substance to the extraction solvent is 1:6-10; And / or, the extraction conditions are: extraction time is 10-15 minutes, extraction temperature is 25-70°C, and pH value is 4-6.

9. The composite dietary composition for improving microcirculation according to claim 1, characterized in that: The composite dietary composition comprises the following components in parts by weight: 30-40 parts of freeze-dried fruit and vegetable composite powder, 1-3 parts of European blueberry concentrated powder, 4-7 parts of Sophora japonica powder, 0.1-5 parts of (3R, 3'R)-dihydroxy-β-carotene, 0.1-0.5 parts of lutein microcapsule powder, 30-40 parts of matcha powder, 0.01-2 parts of fermented ginger powder and 2-7 parts of composite vitamins; The freeze-dried fruit and vegetable composite powder includes at least one of kale powder, hawthorn powder, pomegranate powder, black currant powder, acerola powder, sea buckthorn fruit powder, watermelon powder, carrot powder, tomato powder, spinach powder, barley leaf powder, celery powder or bitter melon powder; The complex vitamin includes at least three of vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B7, vitamin B12, vitamin C, vitamin A, vitamin D and vitamin E.

10. The method for preparing the composite dietary composition for improving microcirculation according to claim 1, characterized in that: include: The freeze-dried fruit and vegetable composite powder, European blueberry concentrated powder, Sophora japonica rice powder, (3R, 3'R)-dihydroxy-β-carotene, lutein microcapsule powder, matcha powder, fermented ginger powder and complex vitamins are mixed with a tablet flow aid, and tablets are formed to obtain the product.

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