Dendrobium nobile soup product capable of relaxing bowels as well as preparation method and application of dendrobium nobile soup product

Abstract

The invention relates to the field of food engineering, and provides a dendrobium nobile soup product capable of relaxing bowels as well as a preparation method and application of the dendrobium nobile soup product. The herba dendrobii soup product is prepared from the following raw materials: herba dendrobii, fructus cannabis and radix astragali seu hedysari, the herba dendrobii, the fructus cannabis and the radix astragali seu hedysari are compounded according to a certain proportion, decocted and centrifuged, and supernate is taken to prepare the herba dendrobii soup product. The dendrobium nobile soup product provided by the invention has the effects of promoting gastrointestinal motility, softening excrement and the like, and particularly has a remarkable effect on promoting the intestine moistening and bowel relaxing activity of zebra fish.

Description

Technical Field

[0001] The invention relates to the field of food engineering, and in particular to a dendrobium soup product for moistening intestines and relieving constipation, and a preparation method and application thereof. Background Art

[0002] Constipation is a major problem that plagues the elderly. About 1 / 3 of the elderly suffer from constipation, which seriously affects their quality of life.

[0003] Constipation refers to a decrease in the number of bowel movements, difficulty in defecation, and dry stool. Normal people defecate 1 to 2 times a day, while constipated patients defecate less than 2 times a week, and defecation is laborious, with hard stool and small amount. Functional constipation can not only cause anal fissures, hemorrhoids and other diseases, but also induce and aggravate cardiovascular and cerebrovascular diseases. In addition, there are abdominal distension, lack of appetite, and abdominal pain before defecation caused by improper use of laxatives. Constipation is a common symptom in the elderly. About 1 / 3 of the elderly suffer from constipation, which seriously affects the quality of life of the elderly. When the elderly defecate too hard, it can lead to changes in coronary artery and cerebral blood flow. Due to the decrease in cerebral blood flow, fainting may occur during defecation. People with insufficient coronary artery blood supply may experience angina pectoris and myocardial infarction. Hypertension can cause cerebrovascular accidents, and can also cause rupture of aneurysms or ventricular aneurysms, detachment of thrombi attached to the heart wall, arrhythmias, and even sudden death. Due to the low tension of the colon muscle layer, megacolon may occur. When straining to defecate, the increased intra-abdominal pressure can cause or aggravate hemorrhoids. Forced defecation can damage the anal canal and cause anal fissures and other perianal diseases. Fecal impaction can cause intestinal obstruction, fecal ulcers, urinary retention, and fecal incontinence.

[0004] Western medicine treatments for functional constipation mainly include dietary intervention and bowel habit intervention, combined with drugs that promote gastrointestinal motility and biofeedback therapy, but the results are poor. Traditional Chinese medicine treatment of this disease can effectively relieve patients' constipation symptoms, and the drug safety is higher than that of Western medicine. However, traditional Chinese medicine also has certain side effects and is difficult to take for a long time. Therefore, it is urgent to develop and research medicinal and edible products that improve constipation symptoms.

[0005] TCM divides constipation into heat constipation, qi constipation, qi deficiency constipation, yin deficiency constipation, and yang deficiency constipation. Among them, heat constipation is mostly caused by gastrointestinal heat accumulation syndrome and intestinal damp-heat syndrome, qi constipation is mostly caused by liver depression and spleen deficiency syndrome and liver depression and qi stagnation syndrome, qi deficiency constipation is mostly caused by spleen and stomach weakness syndrome and spleen and lung qi deficiency syndrome, yin deficiency constipation is mostly caused by yin deficiency and dry intestine syndrome, and yang deficiency constipation is mainly caused by spleen and kidney yang deficiency syndrome. Literature research shows that functional constipation is mainly caused by spleen and kidney yang deficiency, accounting for more than half of the cases, followed by liver and spleen disharmony syndrome, lung and spleen qi deficiency syndrome, liver and kidney yin deficiency syndrome, gastrointestinal heat accumulation syndrome, etc., and yin deficiency type constitution is positively correlated with constipation.

[0006] The Suwen describes the Yin deficiency constitution as follows: "At the age of 67, the three Yang pulses weaken, the face is all burnt, and the hair begins to turn white; at the age of 77, the Ren meridian is weak, the Taichong meridian is weak, the heavenly essence is exhausted, and the earthly passage is blocked, so the body is damaged and there is no child; at the age of 68, the Yang Qi is exhausted, the face is burnt, and the hair turns white; at the age of 78, the liver Qi is weak, the tendons cannot move, the heavenly essence is exhausted, the essence is less, the kidneys are weak, and the body is in a state of extreme; at the age of 88, the teeth and hair fall out." It can be seen that as the middle-aged and elderly people age, their internal organs gradually decline, and the deficiency of Qi and blood makes the large intestine unable to transmit and transform, and it stays in the intestine, causing constipation. The Suwen also believes that "after the age of 40, the Yin Qi is halved, and the daily life is declining." Therefore, as people age, the Yin fluid of the human body is depleted, resulting in dry stools and constipation. This also proves that constipation is related to the Yin deficiency constitution. Summary of the invention

[0007] The invention aims to provide a dendrobium soup product for moistening intestines and relieving constipation, and a preparation method and application thereof.

[0008] In order to achieve the purpose of the present invention, in a first aspect, the present invention provides a composition, wherein the effective ingredients are obtained by water extraction of dendrobium, hemp seed and astragalus, wherein the mass ratio of dendrobium, hemp seed and astragalus is (3-7):(3-7):(1-5), preferably 5:5:3.

[0009] In a second aspect, the present invention provides use of the composition in preparing a product for moistening the intestine and relieving constipation.

[0010] In a third aspect, the present invention provides a dendrobium soup product for moistening the intestine and relieving constipation, which is prepared by mixing fresh dendrobium strips, hemp seeds and astragalus in a mass ratio of (3-7):(3-7):(1-5) (preferably 5:5:3), boiling with water, filtering to obtain a dendrobium soup stock solution, and adding an appropriate amount of auxiliary materials to the stock solution to adjust the taste and transparency, thereby obtaining the dendrobium soup product for moistening the intestine and relieving constipation.

[0011] The auxiliary material can be selected from one or more of rice milk, vitamins, sugar alcohol, chitosan and the like.

[0012] In a fourth aspect, the present invention provides a method for preparing the dendrobium soup product, comprising the following steps: 1) Mix fresh dendrobium strips, hemp seeds and astragalus according to a certain proportion, add water to boil, filter and obtain dendrobium soup stock solution; 2) adding rice milk, vitamin C and xylitol to the stock solution of step 1) to obtain a dendrobium soup solution; 3) The obtained dendrobium soup solution in step 2) is centrifuged and homogenized, and chitosan is added to adjust the transparency to obtain the dendrobium soup processing liquid; 4) The processed liquid in step 3) is canned, sterilized and packaged to obtain the dendrobium soup product with the function of moistening intestines and relieving constipation.

[0013] Further, step 1) includes: S1 weighed raw materials according to the proportion of fresh Dendrobium, hemp seed and Astragalus, wherein the fresh Dendrobium pre-crushed; S2. Boil the fresh Dendrobium strips and 30-60 times (preferably 45 times) of the total weight of the raw materials with pure water for 0.5 h, replenish the lost water (replenish water to 30-60 times, preferably 45 times) the total weight of the raw materials), then add hemp seed and astragalus, boil again for 1.5 h, filter, and obtain the Dendrobium soup stock solution.

[0014] Further, step 2) includes: S3. The rice and water are mixed in a mass ratio of 1: (50-100) (preferably 1: 100), and boiled for 0.5 h to obtain rice milk; S4 was added to the Dendrobium soup stock solution 20% of the total weight of the rice milk, mixed well to obtain a solution I; S5. Add 0.01% of the total weight of solution I of vitamin C to solution I and mix well to obtain solution II; S6. Add 10% xylitol by weight of the total weight of solution II to solution II, mix well, and obtain a dendrobium soup solution.

[0015] Further, step 3) comprises: adding 0.1% chitosan by weight of the total weight of the dendrobium soup solution to the dendrobium soup solution, centrifuging at 8000 r / min for 15 min, and taking the supernatant, which is the dendrobium soup processing liquid.

[0016] Further, step 4) comprises: putting the processed liquid of dendrobium soup into a sealed can, sterilizing it at 60-80° C. for 10-20 min (preferably at 75° C. for 15 min), and then cooling it in ice water.

[0017] In a fifth aspect, the present invention provides the use of the composition or the intestinal moisturizing and laxative dendrobium soup product in the preparation of a preparation for promoting the intestinal moisturizing and laxative activity in zebrafish.

[0018] By means of the above technical solution, the present invention has at least the following advantages and beneficial effects: The present invention provides a dendrobium soup product for moistening the intestine and relieving constipation, and a preparation method and application thereof. The dendrobium soup product is composed of the following raw materials: dendrobium, hemp seed, and astragalus. The dendrobium, hemp seed, and astragalus are compounded in a certain proportion, decocted, centrifuged, and the supernatant is taken to prepare the product. The dendrobium soup product provided by the present invention has the effects of promoting gastrointestinal motility, softening stool, etc., and is particularly effective in promoting the laxative activity of zebrafish. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1The results are as follows: A (blank group), B (model group), C (domperidone group), D (polyethylene glycol group), E (125 μg / ml dendrobium soup group), F (250 μg / ml dendrobium soup group), G (500 μg / ml dendrobium soup group), H (1000 μg / ml dendrobium soup group), I (2000 μg / ml dendrobium soup group).

[0020] Figure 2 The fluorescence intensity of different drug administration groups in the preferred embodiment of the present invention is A (blank group), B (model group), C (comparative example 7), D (comparative example 8), E (125μg / ml dendrobium soup group), F (250μg / ml dendrobium soup group), G (500μg / ml dendrobium soup group), H (1000μg / ml dendrobium soup group), I (2000μg / ml dendrobium soup group). Each group is compared with the model group. .

[0021] Figure 3 and Figure 4 The intestinal fluorescence intensity of different combinations of dendrobium soup in the preferred embodiment of the present invention. Each group was compared with the model group. .

[0022] Figure 5 The effect of Dendrobium soup on 5-HT in the preferred embodiment of the present invention. A (blank group), B (model group), C (domperidone group), D (polyethylene glycol group), E (125μg / ml Dendrobium soup group), F (250μg / ml Dendrobium soup group), G (500μg / ml Dendrobium soup group), H (1000μg / ml Dendrobium soup group), I (2000μg / ml Dendrobium soup group).

[0023] Figure 6 The effect of Dendrobium soup on Ach in the preferred embodiment of the present invention. A (blank group), B (model group), C (domperidone group), D (polyethylene glycol group), E (125μg / ml Dendrobium soup group), F (250μg / ml Dendrobium soup group), G (500μg / ml Dendrobium soup group), H (1000μg / ml Dendrobium soup group), I (2000μg / ml Dendrobium soup group). DETAILED DESCRIPTION

[0024] The invention aims to provide a preparation method and application of a dendrobium soup for moistening intestines and relieving constipation.

[0025] The role of each raw material in the invention: Dendrobium: Dendrobium is known as the first of the "Nine Great Herbs of China". It has the effects of nourishing the stomach and promoting fluid production, nourishing yin and clearing away heat, and has also been scientifically demonstrated in modern medical experimental research. Studies have confirmed that Dendrobium can improve the symptoms of mice with yin deficiency constipation caused by warm and dry drugs. Its mechanism may be related to reducing the expression of iNOS in the colon and thus increasing intestinal motility. In addition, the traditional decoction, ultra-micro full decoction and ultra-micro 50% decoction of Dendrobium can regulate the balance of intestinal microecology and intestinal enzyme activity, and have a good effect on spleen deficiency constipation.

[0026] Constipation is also closely related to intestinal flora, and constipation can occur when there is an obstruction or lesion in any link of the defecation reflex process, so whether the stool is discharged depends on the performance of the gastrointestinal tract. Dendrobium has a protective effect on the gastrointestinal tract, which is mainly related to regulating intestinal flora, promoting gastric acid secretion, improving gastrointestinal motility and inhibiting gastric mucosal damage. Studies have shown that Dendrobium officinale supplements can regulate the composition of intestinal microbiota. In a recent study of Dendrobium officinale polysaccharides, it was found that its specific linear structure of β-endogenous β-1,4-mannanase can restore the diversity of intestinal flora and thus play a protective role in dextran sulfate sodium-induced colitis in mice. In addition, Huoshan Dendrobium polysaccharides can alleviate the clinical symptoms of ulcerative colitis mice by downregulating the expression of TNF-α, IL-6, IL-1β and their related mRNAs, restore the level of short-chain fatty acids, activate G protein-coupled receptors and regulate intestinal flora.

[0027] Dendrobium can also directly stimulate G cells, increase gastrin secretion, and increase serum gastrin concentration. Gastrin can stimulate gastric parietal cells, increase gastric acid secretion, and have a beneficial effect on digestive enzyme activity and nutrient absorption, thereby coordinating gastrointestinal function. The polysaccharide components in Dendrobium include D-glucose, mannose, galactose, D-xylose, L-arabinose, galactose and other monosaccharide components, which can promote the secretion of cytokines to varying degrees, including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and nitric oxide (NO). The polysaccharides from Dendrobium nobile, Dendrobium tenuifolium, and Dendrobium liptatum have an excitatory effect on the intestine and can increase the contraction amplitude of the intestine; Dendrobium officinale, Dendrobium nigrofurum, Dendrobium slender-stemmed, Dendrobium sheathed, and Dendrobium tasselled first inhibit intestinal activity, which can be restored to the level before administration after a few minutes; the contraction amplitude of Dendrobium uncinate is slightly reduced; Dendrobium fasciatum significantly reduces the tension of spontaneous intestinal activity, eliminates the rhythm, and puts the intestine in a state of complete paralysis, and can antagonize the excitatory effect of acetylcholine on the intestine.

[0028] Hemp seed: Hemp seed is the dried mature fruit of the plant Cannabis sativa, which belongs to the Moraceae family. It is a traditional food and medicine crop. It tastes sweet and is neutral in nature. It belongs to the spleen, stomach, and large intestine meridians. It has the effects of moistening the intestines, relieving constipation, and replenishing qi. It is often used for blood deficiency, fluid deficiency, and dry intestinal constipation. It is an important medicine for treating functional constipation. Studies have shown that after the constipated mice were given a Yin-nourishing and intestine-moistening prescription containing hemp seed, the water content in the feces was significantly increased compared with the natural recovery group. After immunohistochemical detection of its colon tissue, it was found that the increase in fecal water content of constipated mice may be achieved by affecting the expression of AQP3 gene, C-Kit gene and protein. Wu Bensheng et al. used a Wenyang Yiqi prescription containing hemp seed to administer to the constipated mice. Using immunohistochemistry and other techniques, they found that the Wenyang Yiqi prescription can reduce the expression of intestinal AQP3 and reduce the reabsorption of water in the intestinal cavity to achieve the purpose of treatment.

[0029] Astragalus: Astragalus has a very good effect on qi deficiency constipation. Studies have shown that the combination of Astragalus soup and compound Lactobacillus acidophilus in the treatment of post-chemotherapy constipation can reorganize the intestinal flora and improve constipation. In addition, Astragalus has a very good effect of replenishing qi and strengthening the spleen. The main drug pairs include Astragalus-chicken gizzard, Astragalus-raw malt, Astragalus-yam, etc. The main medicinal recipes include Four Gentlemen Steamed Duck, Spleen-strengthening and Qi-invigorating Congee, Eight Treasure Rice, etc.

[0030] The present invention adopts the following technical solution: Preparation of raw materials: (1) Weigh fresh dendrobium strips, hemp seed, and astragalus in a mass ratio of 5:5:3, crush the fresh dendrobium strips, and boil them with purified water 45 times the total weight of the raw materials for 0.5 h to make up for the lost water; (2) Add hemp seed and astragalus, and simmer for another 1.5 h. Collect the supernatant for later use.

[0031] In a preferred embodiment, the method specifically comprises: S1. Weigh the raw materials of fresh dendrobium, hemp seed and astragalus according to the mass ratio of 5:5:3, and crush the fresh dendrobium. Prepare 45 times the total weight of the raw materials of purified water for standby use.

[0032] S2. Decoction the fresh Dendrobium officinale strips with water for 0.5 h to make up for the lost water, then add hemp seed and astragalus, decoct for 1.5 h, filter, centrifuge at 8000 r / min for 15 min, take the supernatant, and obtain the Dendrobium officinale soup solution.

[0033] S3. Rice and water were mixed in a mass ratio of 1:100 and boiled for 0.5 h to obtain rice slurry.

[0034] S4. Add 20% of the total weight of the stock solution of rice milk to the stock solution of Dendrobium soup, mix well, and obtain solution I.

[0035] S5. Add 0.1% of the total weight of solution I of vitamin C to solution I and mix well to obtain solution II.

[0036] S6. Add 10% xylitol by weight of the total weight of solution II to solution II, mix well, and obtain a dendrobium soup solution.

[0037] S7. Add 0.1% chitosan by weight of the total weight of the dendrobium soup solution to the dendrobium soup solution, centrifuge at 8000 r / min for 15 min, and take the supernatant, which is the dendrobium soup processing liquid.

[0038] S8. Pour the processed liquid of dendrobium soup into a sealed can, sterilize it at 75℃ for 15min, and then cool it in ice water.

[0039] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products.

[0040] In the following examples and comparative examples, dendrobium, hemp seed and astragalus are all commercially available high-quality materials, which are then decocted to obtain corresponding extracts; auxiliary materials such as xylitol are directly purchased from the market.

[0041] Example 1 1. Raw material preparation: (1) Weigh fresh dendrobium strips, hemp seed, and astragalus in a mass ratio of 5:5:3. Crush the fresh dendrobium strips and boil them with 45 times the total weight of pure water for 0.5 h to make up for the lost water. (2) Add hemp seed and astragalus and simmer for another 1.5 h. Collect the supernatant for later use.

[0042] Specifically, the method comprises: S1. Weigh the raw materials of fresh dendrobium, hemp seed and astragalus according to the mass ratio of 5:5:3, and crush the fresh dendrobium. Prepare 45 times the total weight of the raw materials of purified water for standby use.

[0043] S2. Decoction the fresh Dendrobium officinale strips with water for 0.5 h to make up for the lost water, then add hemp seed and astragalus, decoct for 1.5 h, filter, centrifuge at 8000 r / min for 15 min, take the supernatant, and obtain the Dendrobium officinale soup solution.

[0044] S3. Rice and water were mixed in a mass ratio of 1:100 and boiled for 0.5 h to obtain rice slurry.

[0045] S4. Add 20% of the total weight of the stock solution of rice milk to the Dendrobium soup stock solution and mix well to obtain a solution I.

[0046] S5. Add 0.1% of the total weight of solution I of vitamin C to solution I and mix well to obtain solution II.

[0047] S6. Add 10% xylitol by weight of the total weight of solution II to solution II, mix well, and obtain a dendrobium soup solution.

[0048] S7. Add 0.1% chitosan by weight of the total weight of the dendrobium soup solution to the dendrobium soup solution, centrifuge at 8000 r / min for 15 min, and take the supernatant, which is the dendrobium soup processing liquid.

[0049] S8. Pour the processed liquid of Dendrobium soup into a sealed can, sterilize it at 75℃ for 15min, and then cool it in ice water.

[0050] Example 2 This comparative example provides a dendrobium soup a, which is different from Example 1 only in that the ratio of dendrobium: hemp seed: astragalus is 7:7:5.

[0051] Example 3 This comparative example provides a dendrobium soup B, which is different from Example 1 only in that the ratio of dendrobium: hemp seed: astragalus is 5:3:3.

[0052] Example 4 This comparative example provides a dendrobium soup C, which is different from Example 1 only in that the ratio of dendrobium: hemp seed: astragalus is 3:5:3.

[0053] Example 5 This comparative example provides a dendrobium soup d, which is different from Example 1 only in that the ratio of dendrobium: hemp seed: astragalus is 3:3:5.

[0054] Example 6 This comparative example provides a dendrobium soup E, which is different from Example 1 only in that the ratio of dendrobium: hemp seed: astragalus is 3:3:1.

[0055] Comparative Example 1 (1) Take 18g of Astragalus, 15g of Dendrobium, 12g of Lycium barbarum, 5g of Licorice, 12g of Polygonatum, 12g of Cannabis Sativa, 12g of Angelica and 6g of Cimicifuga. After the Dendrobium is crushed, prepare 45 times the total weight of pure water for later use.

[0056] (2) Decoction the fresh dendrobium strips with water for 0.5 h to make up for the lost water, then add other raw materials, decoct for 1.5 h, filter, centrifuge at 8000 r / min for 15 min, take the supernatant, and obtain the Huangqi Shihu Tang solution. This comparative example provides an Huangqi Shihu Tang for treating constipation, see CN105521300A, which has more medicinal flavor than Shihu Tang.

[0057] Comparative Example 2 (1) Radish seeds 10g, chicken gizzard lining 10g, bergamot 10g, roasted malt 10g, Shenqu 6g, dendrobium 15g, betel nut 10g, Citrus aurantium 10g, Chinese angelica 10g, Cistanche deserticola 10g, hemp seed 12g, Prunus mume seed 15g, astragalus 9g, licorice 5g.

[0058] (2) Add 45 times of water and boil twice, each time for 1 hour, combine the filtrate, and concentrate to a relative density of 1.05-1.15 for use, to obtain a paste for treating constipation in children. This comparative example provides a solution for treating constipation in children, which is the content of Example 1 in the specification of CN103405689A, and the medicinal taste is more than that of Dendrobium soup.

[0059] Comparative Example 3 This comparative example provides a hemp seed and astragalus decoction, which is different from Example 1 only in that the amount of dendrobium added is 0.

[0060] Comparative Example 4 This comparative example provides a dendrobium and astragalus decoction, which is different from Example 1 only in that the amount of hemp seed is 0.

[0061] Comparative Example 5 This comparative example provides a dendrobium and hemp seed decoction, which is different from Example 1 only in that the amount of astragalus is 0.

[0062] Comparative Example 6 This comparative example provides a dendrobium decoction, which is different from Example 1 only in that the dosage of hemp seed and astragalus is 0. Fresh dendrobium strips are boiled with 45 times water for 2 hours, filtered, and the decoction is obtained.

[0063] Comparative Example 7 This comparative example provides a hemp seed decoction, which is different from Example 1 only in that the amount of dendrobium and astragalus added is 0. Hemp seed and water are boiled for 1.5 hours and filtered to obtain the decoction.

[0064] Comparative Example 8 This comparative example provides a decoction of Astragalus membranaceus, which is different from Example 1 only in that the amount of Dendrobium officinale and Fructus Cannabis Sativa added is 0. Astragalus membranaceus and water are boiled for 1.5 hours, filtered, and the decoction is obtained.

[0065] Comparative Example 9 This comparative example provides a Ma Zi Ren Wan product (purchased from Tong Ren Tang), which is different from a common Chinese patent medicine on the market.

[0066] Comparative Example 10 This comparative example provides a domperidone positive drug (purchased from Xi'an Janssen Pharmaceutical Co., Ltd.), which is different in that it is a common Chinese patent medicine available on the market.

[0067] Comparative Example 11 This comparative example provides polyethylene glycol (purchased from Xi'an Janssen Pharmaceutical Co., Ltd.), which is different from polyethylene glycol in that it is a common Chinese patent medicine available on the market.

[0068] The following groups were provided for evaluation and testing of each embodiment and comparative example (Table 1): Table 1 Sample composition of each embodiment and control group

[0069] 1. Test methods 1. Dendrobium soup dosage selection: 180 7dpf zebrafish were selected as experimental subjects and placed in 6-well plates and divided into 9 groups, 20 in each group. One group was the normal control group, which was induced by Nile red only, and the other 8 groups used the Nile red-loperamide hydrochloride method to establish the zebrafish intestinal constipation model. The concentration of Nile red was 10μg / L, and the concentration of loperamide hydrochloride was 10μg / ml. The intestines of all zebrafish were examined under a microscope after 24 hours.

[0070] The 8 groups were 1 model group, 1 model + positive drug control group (10μg / ml domperidone and 10μg / ml polyethylene glycol), 1 model + drug control group (125μg / ml dendrobium soup), 1 model + drug control group (250μg / ml dendrobium soup), 1 model + drug control group (500μg / ml dendrobium soup), 1 model + drug control group (1000μg / ml dendrobium soup), 1 model + drug control group (2000μg / ml dendrobium soup), and washed three times with pure water after 24 hours. The fluorescence intensity of zebrafish intestine was observed under an inverted fluorescence microscope. The results are as follows Figure 1 shown.

[0071] 2. Study on the effect of lubricating the intestine and relieving constipation: 200 zebrafish at 7dpf were selected as experimental subjects, placed in a 6-well plate and divided into 10 groups, with 20 fish in each group. One group was the normal control group, which was induced by Nile red only, and the other 8 groups used the Nile red-loperamide hydrochloride method to establish an intestinal constipation model of zebrafish. The intestines of all zebrafish were examined under a microscope after 24 hours. The 16 groups were 1 normal control group, 1 model group, and 2 positive drug groups, and the rest were Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, Comparative Example 5, Comparative Example 6, Comparative Example 7, Comparative Example 8, Comparative Example 9, Comparative Example 10, Comparative Example 11, Example 1, Example 2, Example 3, Example 4, Example 5, and Example 6. The intestinal fluorescence of zebrafish was observed under an inverted fluorescence microscope, as shown in Figure 2 As shown, Image J calculated the fluorescence intensity to obtain the data of laxative effect, such as Figure 3 and Figure 4 shown.

[0072] 3. Determination of gastrointestinal hormone content: Randomly select 20 fish from six-well plates of different groups, rinse with PBS three times, put them into 0.2ml centrifuge tubes respectively, and adjust the volume to 0.1ml. Put 2 2mm grinding beads in the centrifuge tube and grind it in a low-temperature tissue grinder at 4℃, grind for 60s every 15s, and grind three times. Centrifuge at 4℃, 12000rmp for 10min, put it on ice and set aside. Use the enzyme-linked immunosorbent assay kit and operate strictly according to the instructions. Determination of acetylcholine (Ach) content: Use the zebrafish acetylcholine (Ach) enzyme-linked immunosorbent assay kit for analysis, and operate strictly according to the instructions. Determination of 5-hydroxytryptamine (5-HT) content: Use the zebrafish 5-HT enzyme-linked immunosorbent assay kit and operate strictly according to the instructions. The kits used in this experiment are all from ELISA company. The results are as follows Figure 5 and Figure 6 shown.

[0073] 2. Experimental Results Table 2 Results of the intestinal moistening and laxative test in each implementation group and the comparison group Table 3 Gastrointestinal hormone content in different concentrations of Dendrobium soup The smaller the fluorescence intensity, the faster the zebrafish excretes Nile red, and the better the laxative effect. The smaller the maximum peak time of gastrointestinal motility, the faster the gastrointestinal motility, and the better the laxative effect.

[0074] Combination table 2, table 3 can know, compared with model group, experimental group fluorescence intensity all reduces, gastrointestinal motility maximum wave peak generation time all becomes smaller, explanation herb, hemp seed and astragalus have certain laxative effect, wherein embodiment 1 reduces most obviously, can think three compatibility after effect enhancement. 4 experimental groups are compared, experimental group 1 fluorescence intensity declines most obviously, shows that experimental group 1 laxative effect is best. Comparison group 1, 2 are compared with comparative example 5, comparative example 5 effect is better than the first two, explanation herb and hemp seed compatibility after laxative effect is better. In the same way, comparison group 1, 2, 3 are compared with embodiment 1, explanation herb, hemp seed, astragalus collocation after effect is stronger than each single taste component laxative effect.

[0075] In the preferred embodiment, it was found that the dendrobium soup had a good laxative effect when it was in a ratio of 5:5:3 and 7:7:5, but at 7:7:5, zebrafish fry died, and white floccules were observed at the tail end of the intestine of the dead zebrafish under a microscope, and its safety is questionable. When the ratio was 5:5:3 at 500 μg / ml, the fry was still in good condition, so the dendrobium soup was selected as the optimal group when the ratio of dendrobium: hemp seed: astragalus = 5:5:3.

[0076] Comparative Examples 6, 7, and 8 were compared and found that Comparative Example 7 had a laxative effect that was significantly better than the other two groups, indicating that among the three single-flavor drugs, Fructus Cannabis had the best laxative effect, followed by Herba Dendrobii, and Herba Astragali had the weakest laxative effect. Comparative Examples 3, 4, and 5 were compared with each group of Example 1 and found that Example 1 was better than the other three groups, indicating that Herba Dendrobii, Fructus Cannabis, and Herba Astragali were all combined to achieve a better effect than the two-by-two comparison groups. Among them, Comparative Example 3 was better than Comparative Examples 4 and 5, indicating that after Herba Dendrobii and Fructus Cannabis were combined, the effect was better than the Herba Dendrobii and Herba Astragali compatibility groups, and the Herba Hempis and Herba Astragali compatibility groups.

[0077] Compared with other patents containing dendrobium, hemp seed and astragalus, the effect of dendrobium soup is still good. For example, compared with the effect of dendrobium soup group, the effect is slightly weaker than that of comparative examples 1 and 2, indicating that the effect of dendrobium soup is better than that of other groups with additional Chinese medicine when dendrobium: hemp seed: astragalus = 5:5:3. Compared with the Chinese patent medicine Maziren Pill, the two have similar effects of moistening the intestine and relaxing the bowels, indicating that the whole prescription of dendrobium soup has good effect of moistening the intestine and relaxing the bowels when dendrobium: hemp seed: astragalus = 5:5:3. Compared with comparative examples 9 and 10, it is shown that dendrobium soup temporarily does not reach the level of domperidone, but it is slightly superior to polyethylene glycol, and the whole prescription is both medicine and food, and has less toxicity.

[0078] The above experimental results show that the combination of all the ingredients of Dendrobium Soup increases the expression of gastrointestinal hormones 5-HT and Ach, improves gastrointestinal motility, and achieves a better purpose of moisturizing the intestine and promoting bowel movements compared with uncombined or incompletely combined ingredients.

[0079] Although the present invention has been described in detail above with general descriptions and specific embodiments, it is obvious to those skilled in the art that some modifications or improvements can be made based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection claimed by the present invention.

Claims

1. A composition, characterized in that The effective ingredients are extracted from dendrobium, hemp seed and astragalus by water, wherein the mass ratio of dendrobium, hemp seed and astragalus is (3-7):(3-7):(1-5), preferably 5:5:

3.

2. Use of the composition according to claim 1 in preparing a product for moistening the intestine and relieving constipation.

3. A dendrobium soup product for moistening the intestines and relieving constipation, characterized in that: The method comprises the following steps: mixing fresh dendrobium strips, hemp seeds and astragalus according to a mass ratio of (3-7):(3-7):(1-5), boiling with water, filtering to obtain a dendrobium soup stock solution, adding a proper amount of auxiliary materials to the stock solution to adjust the taste and transparency, and obtaining a dendrobium soup product for moistening the intestine and facilitating bowel movements.

4. The dendrobium soup product according to claim 3, characterized in that: The auxiliary material is selected from one or more of rice milk, vitamins, sugar alcohol and chitosan.

5. The method for preparing the dendrobium soup product according to claim 4, characterized in that: The following steps are involved: 1) Mix fresh dendrobium strips, hemp seeds and astragalus according to a certain proportion, add water to boil, filter and obtain dendrobium soup stock solution; 2) adding rice milk, vitamin C and xylitol to the stock solution of step 1) to obtain a dendrobium soup solution; 3) The obtained dendrobium soup solution in step 2) is centrifuged and homogenized, and chitosan is added to adjust the transparency to obtain the dendrobium soup processing liquid; 4) The processed liquid in step 3) is canned, sterilized and packaged to obtain the dendrobium soup product with the function of moistening intestines and relieving constipation.

6. The method according to claim 5, characterized in that Step 1) includes: S1 weighed raw materials according to the proportion of fresh Dendrobium, hemp seed and Astragalus, wherein the fresh Dendrobium pre-crushed; S2. Decoction the fresh Dendrobium officinale strips and purified water 30 to 60 times the total weight of the raw materials for 0.5 h to make up for the lost water, then add hemp seed and astragalus, decoct again for 1.5 h, filter, and obtain the Dendrobium officinale soup stock solution.

7. The method according to claim 6, characterized in that Step 2) includes: S3. The rice and water were mixed in a mass ratio of 1: (50-100) and boiled for 0.5h to obtain rice milk; S4 was added to the Dendrobium soup stock solution 20% of the total weight of the rice milk, mixed well to obtain a solution I; S5. Add 0.1% of the total weight of solution I of vitamin C to solution I and mix well to obtain solution II; S6. Add 10% xylitol by weight of the total weight of solution II to solution II, mix well, and obtain a dendrobium soup solution.

8. The method according to claim 7, characterized in that Step 3) comprises: adding chitosan in an amount of 0.1% by weight of the total weight of the dendrobium soup solution to the dendrobium soup solution, centrifuging at 8000 r / min for 15 minutes, and taking the supernatant, which is the dendrobium soup processing liquid.

9. The method according to claim 8, characterized in that Step 4) includes: putting the processed liquid of dendrobium soup into a sealed can, sterilizing it at 65-85° C. for 10-20 minutes, and then cooling it in ice water.

10. Use of the composition of claim 1 or the laxative dendrobium soup product of claim 3 or 4 in the preparation of a preparation for promoting laxative activity in zebrafish.

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