Preparation method of lotus leaf / chrysanthemum composite beverage with effects of lowering lipid and improving eyesight

Abstract

The invention discloses a preparation method of a lotus leaf / chrysanthemum composite beverage with lipid-lowering and eyesight-improving effects. The preparation method comprises the following steps: pre-treating lotus leaves; performing crushing; performing enzymolysis; performing extraction; freezing and clarifying; treating a PVDF (Polyvinylidene Fluoride) membrane; performing debitterizing; performing sterilization; the PVDF membrane treatment technology is applied, and the problems that macromolecular substances are difficult to dissolve and easy to precipitate in the processing process of the lotus leaf and chrysanthemum tea drink are hopefully solved. The water-soluble dietary fiber resistant dextrin is used as a debitterizing substance, so that the taste of the lotus leaf and chrysanthemum tea beverage is improved, and the nutritional effect of the beverage can also be increased.

Description

Technical Field

[0001] The invention discloses a functional beverage, in particular to a preparation method of a lotus leaf / chrysanthemum composite beverage with lipid-lowering and eyesight-improving effects. Background Art

[0002] Lotus leaves are rich in nutrients, containing protein, carbohydrates, vitamin C, fat, niacin, thiamine, carotene and other substances. In addition, they also contain trace elements such as calcium, phosphorus, iron, and a variety of alkaloids and flavonoid compounds. They have the effects of clearing away heat and dampness, promoting the flow of clear yang, promoting the production of body fluids and quenching thirst, removing blood stasis and stopping bleeding, and can cure dizziness, water vapor edema, vomiting blood and other symptoms. Long-term drinking of lotus leaf tea has the effects of reducing fat and weight, and preventing arteriosclerosis. At present, the market size of lotus leaf tea is constantly expanding. Because lotus leaves are rich in carbohydrate macromolecules and alkaloids, the production and processing process generally has the problems of bitter taste and more precipitation.

[0003] Chrysanthemum has the effect of clearing the liver and improving eyesight. It is effective in treating symptoms such as red, swollen and painful eyes, dizziness and vertigo caused by hyperactivity of liver yang. At the same time, it can also relieve eye problems such as eye fatigue, blurred vision, dry and painful eyes, and has a significant effect on blurred vision caused by long-term use of the eyes or insufficient liver and kidney essence and blood. Chrysanthemum is rich in vitamins, trace elements and minerals. These nutrients play an important role in maintaining the normal physiological functions of the human body. Drinking chrysanthemum tea appropriately can effectively supplement these nutrients and promote physical health. Summary of the invention

[0004] The present invention proposes to use lotus leaf and chrysanthemum as raw materials, and through process innovation optimization and reasonable matching, develop a lotus leaf and chrysanthemum tea beverage suitable for people of all ages to drink, which has the effects and functions of clearing away heat and relieving summer heat, improving eyesight and lowering blood pressure, losing weight and slimming, anti-oxidation and improving sleep.

[0005] The present invention uses PVDF membrane processing technology to solve the problem that macromolecular substances are difficult to dissolve and easy to precipitate during the processing of lotus leaf chrysanthemum tea beverages. The water-soluble dietary fiber resistant dextrin is used as a debittering substance to improve the taste of lotus leaf chrysanthemum tea beverages and increase their nutritional efficacy.

[0006] 1. Lotus leaf pretreatment Choose fresh lotus leaves that are large, complete, green, and spotless. Generally, lotus leaves that have just grown out of the water for 2 to 3 months are suitable. Use tap water to wash away the mud and impurities on the surface of the lotus leaves and drain the water. Place the lotus leaves in a 100℃ water bath steam for 40-60 seconds.

[0007] 2. Broken lotus leaves The pretreated lotus leaves were crushed into small pieces with a size of 0.1 cm*0.1 cm in length.

[0008] 3. Enzymatic hydrolysis The crushed lotus leaves are added with water according to a solid-liquid ratio of 1:5-10, and cellulase is added for enzymolysis. Then, quinoa powder is added, and neutral protease is added for enzymolysis to obtain an enzymolysis solution.

[0009] 4. Extraction Add the enzymatic hydrolysate and chrysanthemum solids in a ratio of 1:1 to the extraction tank, add deionized water 50-60 times the weight of the solids, set the extraction temperature to 80-90°C, and the extraction time to 1.5 hours. After the extraction is completed, filter with double-layer gauze to remove the residue.

[0010] 5. Freeze clarification The filtrate was rapidly cooled to 4°C and stored for 24 hours to allow the precipitate to precipitate naturally, and the precipitate was filtered out to obtain a clear solution.

[0011] 6. PVDF membrane treatment The above solution was filtered using a 5000 D PVDF nanofiltration membrane.

[0012] 8. Escape from suffering Add water-soluble dietary fiber resistant dextrin to the above clarified liquid and stir.

[0013] 9. Sterilization The composite liquid is subjected to high-temperature instantaneous sterilization treatment at a temperature of 137° C. for 30 seconds, thereby preparing a lotus leaf / chrysanthemum composite beverage having lipid-lowering and eye-improving effects.

[0014] Furthermore, in step (3), the amount of cellulase added is 0.1%-0.2% of the mass of the lotus leaf, the enzymolysis temperature is 30-50°C, and the time is 1-4 hours.

[0015] Furthermore, in step (3), the amount of neutral protease added is 0.1-0.3% of the mass of the lotus leaf, the enzymatic hydrolysis temperature is 30-50°C, and the time is 1-4 hours.

[0016] Furthermore, in step (3), the mass ratio of the added amount of quinoa powder to the lotus leaf is 0.1-0.2:1.

[0017] Furthermore, in step (8), the amount of dextrin added is 0.5-0.8% of the mass of the clarified liquid.

[0018] Beneficial Effects The present invention utilizes enzymatic hydrolysis to maximize the development of effective ingredients in lotus leaves. After the protein in quinoa is hydrolyzed, not only the nutritional components can be increased, but also the taste of the beverage can be improved. At the same time, the PVDF membrane treatment technology is used to solve the problem of poor solubility and easy precipitation of macromolecular substances in the processing of lotus leaf beverages.

[0019] The present invention adopts water-soluble dietary fiber resistant dextrin as a debittering substance, thereby improving the taste of the lotus leaf chrysanthemum tea beverage and increasing its nutritional efficacy. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 The following is an evaluation comparison of the embodiments and comparative examples. DETAILED DESCRIPTION

[0021] The following is a detailed description of an embodiment of the present invention. This embodiment is implemented on the premise of the technical solution of the present invention, and a detailed implementation method and a specific operation process are given, but the protection scope of the present invention is not limited to the following embodiment.

[0022] The chemicals used in the present invention are all commercially available.

[0023] Example 1 1. Lotus leaf pretreatment Choose fresh lotus leaves that are large, complete, green, and spotless. Generally, lotus leaves that have just grown out of the water for 2 to 3 months are suitable. Use tap water to wash away the mud and impurities on the surface of the lotus leaves and drain the water. Place the lotus leaves in a 100℃ water bath steam for 40 seconds.

[0024] 2. Broken lotus leaves The pretreated lotus leaves were crushed into small pieces with a size of 0.1 cm*0.1 cm in length.

[0025] 3. Enzymatic hydrolysis The crushed lotus leaves were added with water at a solid-liquid ratio of 1:10, and 0.1% of cellulase by mass of the lotus leaves was added, and the enzymolysis temperature was 35°C for 2 hours. Then, quinoa powder was added (the mass ratio of quinoa powder to lotus leaves was 0.1:1), and then 0.2% of neutral protease by mass of the lotus leaves was added for enzymolysis, and the enzymolysis temperature was 50°C for 4 hours to obtain an enzymolysis solution.

[0026] 4. Extraction The enzymatic hydrolysate and chrysanthemum solids were mixed in a ratio of 1:1 and added to the extraction tank, and deionized water was added at 50 times the weight of the solids. The extraction temperature was set at 90°C and the extraction time was 1.5 hours. After the extraction was completed, the residue was filtered out with double-layer gauze.

[0027] 5. Freeze clarification The filtrate was rapidly cooled to 4°C and stored for 24 hours to allow the precipitate to precipitate naturally, and the precipitate was filtered out to obtain a clear solution.

[0028] 6. PVDF membrane treatment The above solution was filtered using a 5000 D PVDF nanofiltration membrane.

[0029] 8. Escape from suffering Add 0.5% water-soluble dietary fiber resistant dextrin based on the weight of the clarified liquid to the clarified liquid and stir.

[0030] 9. Sterilization The composite liquid is subjected to high-temperature instantaneous sterilization treatment at a temperature of 137° C. for 30 seconds, thereby preparing a lotus leaf / chrysanthemum composite beverage having lipid-lowering and eye-improving effects.

[0031] Example 2 1. Lotus leaf pretreatment Choose fresh lotus leaves that are large, complete, green, and spotless. Generally, lotus leaves that have just grown out of the water for 2 to 3 months are suitable. Use tap water to wash away the mud and impurities on the surface of the lotus leaves and drain the water. Place the lotus leaves in a 100℃ water bath steam for 60 seconds.

[0032] 2. Broken lotus leaves The pretreated lotus leaves were crushed into small pieces with a size of 0.1 cm*0.1 cm in length.

[0033] 3. Enzymatic hydrolysis The crushed lotus leaves were added with water at a solid-liquid ratio of 1:5, and 0.1% cellulase based on the mass of the lotus leaves was added. The enzymatic hydrolysis temperature was 35°C and the time was 2 hours. Then, quinoa powder was added (the mass ratio of quinoa powder to lotus leaves was 0.2:1), and then neutral protease was added for enzymatic hydrolysis at 50°C for 4 hours to obtain an enzymatic hydrolyzate.

[0034] 4. Extraction The enzymatic hydrolysate and chrysanthemum solids were mixed in a ratio of 1:1 and added to the extraction tank, and deionized water was added at 60 times the weight of the solids. The extraction temperature was set at 90°C and the extraction time was 1.5 hours. After the extraction was completed, the residue was filtered out with double-layer gauze.

[0035] 5. Freeze clarification The filtrate was rapidly cooled to 4°C and stored for 24 hours to allow the precipitate to precipitate naturally, and the precipitate was filtered out to obtain a clear solution.

[0036] 6. PVDF membrane treatment The above solution was filtered using a 5000 D PVDF nanofiltration membrane.

[0037] 8. Escape from suffering Add 0.8% water-soluble dietary fiber resistant dextrin based on the mass of the clarified liquid to the clarified liquid and stir.

[0038] 9. Sterilization The composite liquid is subjected to high-temperature instantaneous sterilization treatment at a temperature of 137° C. for 30 seconds, thereby preparing a lotus leaf / chrysanthemum composite beverage having lipid-lowering and eye-improving effects.

[0039] Example 3 1. Lotus leaf pretreatment Choose fresh lotus leaves that are large, complete, green, and spotless. Generally, lotus leaves that have just grown out of the water for 2 to 3 months are suitable. Use tap water to wash away the mud and impurities on the surface of the lotus leaves and drain the water. Place the lotus leaves in a 100℃ water bath steam for 50 seconds.

[0040] 2. Broken lotus leaves The pretreated lotus leaves were crushed into small pieces with a size of 0.1 cm*0.1 cm in length.

[0041] 3. Enzymatic hydrolysis The crushed lotus leaves were added with water at a solid-liquid ratio of 1:8, and 0.2% cellulase based on the mass of the lotus leaves was added. The enzymolysis temperature was 35°C and the time was 2 hours. Then, quinoa powder was added (the mass ratio of quinoa powder to lotus leaves was 0.2:1), and then 0.3% neutral protease based on the mass of the lotus leaves was added for enzymolysis at 50°C for 4 hours to obtain an enzymolysis solution.

[0042] 4. Extraction The enzymatic hydrolysate and chrysanthemum solids were mixed in a ratio of 1:1 and added to the extraction tank, and deionized water was added at 50 times the weight of the solids. The extraction temperature was set at 90°C and the extraction time was 1.5 hours. After the extraction was completed, the residue was filtered out with double-layer gauze.

[0043] 5. Freeze clarification The filtrate was rapidly cooled to 4°C and stored for 24 hours to allow the precipitate to precipitate naturally, and the precipitate was filtered out to obtain a clear solution.

[0044] 6. PVDF membrane treatment The above solution was filtered using a 5000 D PVDF nanofiltration membrane.

[0045] 8. Escape from suffering Add 0.6% water-soluble dietary fiber resistant dextrin based on the mass of the clarified liquid to the clarified liquid and stir.

[0046] 9. Sterilization The composite liquid is subjected to high-temperature instantaneous sterilization treatment at a temperature of 137° C. for 30 seconds, thereby preparing a lotus leaf / chrysanthemum composite beverage having lipid-lowering and eye-improving effects.

[0047] Comparative Example 1 1. Lotus leaf pretreatment Choose fresh lotus leaves that are large, complete, green, and spotless. Generally, lotus leaves that have just grown out of the water for 2 to 3 months are suitable. Use tap water to wash away the mud and impurities on the surface of the lotus leaves and drain the water. Place the lotus leaves in a 100℃ water bath steam for 50 seconds.

[0048] 2. Broken lotus leaves The pretreated lotus leaves were crushed into small pieces with a size of 0.1 cm*0.1 cm in length.

[0049] 3. Mix Add the crushed lotus leaves to quinoa powder (the mass ratio of quinoa powder to lotus leaves is 0.2:1) and mix well.

[0050] 4. Extraction The mixture and chrysanthemum solids were mixed in a ratio of 1:1 and added to the extraction tank, and deionized water was added at 50 times the weight of the solids. The extraction temperature was set at 90°C and the extraction time was 1.5 hours. After the extraction was completed, the residue was filtered out with double-layer gauze.

[0051] 5. Freeze clarification The filtrate was rapidly cooled to 4°C and stored for 24 hours to allow the precipitate to precipitate naturally, and the precipitate was filtered out to obtain a clear solution.

[0052] 6. PVDF membrane treatment The above solution was filtered using a 5000 D PVDF nanofiltration membrane.

[0053] 8. Escape from suffering Add 0.6% water-soluble dietary fiber resistant dextrin based on the mass of the clarified liquid to the clarified liquid and stir.

[0054] 9. Sterilization The composite liquid is subjected to high-temperature instantaneous sterilization treatment at a temperature of 137° C. for 30 seconds, thereby preparing a lotus leaf / chrysanthemum composite beverage having lipid-lowering and eye-improving effects.

[0055] Comparative Example 2 1. Lotus leaf pretreatment Choose fresh lotus leaves that are large, complete, green, and spotless. Generally, lotus leaves that have just grown out of the water for 2 to 3 months are suitable. Use tap water to wash away the mud and impurities on the surface of the lotus leaves and drain the water. Place the lotus leaves in a 100℃ water bath steam for 50 seconds.

[0056] 2. Broken lotus leaves The pretreated lotus leaves were crushed into small pieces with a size of 0.1 cm*0.1 cm in length.

[0057] 3. Enzymatic hydrolysis The crushed lotus leaves were added with water at a solid-liquid ratio of 1:8, and 0.2% cellulase based on the weight of the lotus leaves was added. The enzymolysis temperature was 35° C. and the time was 2 hours to obtain an enzymolysis solution.

[0058] 4. Extraction The enzymatic hydrolysate and chrysanthemum solids were mixed in a ratio of 1:1 and added to the extraction tank, and deionized water was added at 50 times the weight of the solids. The extraction temperature was set at 90°C and the extraction time was 1.5 hours. After the extraction was completed, the residue was filtered out with double-layer gauze.

[0059] 5. Freeze clarification The filtrate was rapidly cooled to 4°C and stored for 24 hours to allow the precipitate to precipitate naturally, and the precipitate was filtered out to obtain a clear solution.

[0060] 6. PVDF membrane treatment The above solution was filtered using a 5000 D PVDF nanofiltration membrane.

[0061] 8. Escape from suffering Add 0.6% water-soluble dietary fiber resistant dextrin based on the mass of the clarified liquid to the clarified liquid and stir.

[0062] 9. Sterilization The composite liquid is subjected to high-temperature instantaneous sterilization treatment at a temperature of 137° C. for 30 seconds, thereby preparing a lotus leaf / chrysanthemum composite beverage having lipid-lowering and eye-improving effects.

[0063] Comparative Example 3 1. Lotus leaf pretreatment Choose fresh lotus leaves that are large, complete, green, and spotless. Generally, lotus leaves that have just grown out of the water for 2 to 3 months are suitable. Use tap water to wash away the mud and impurities on the surface of the lotus leaves and drain the water. Place the lotus leaves in a 100℃ water bath steam for 50 seconds.

[0064] 2. Broken lotus leaves The pretreated lotus leaves were crushed into small pieces with a size of 0.1 cm*0.1 cm in length.

[0065] 3. Enzymatic hydrolysis The crushed lotus leaves were added with water at a solid-liquid ratio of 1:8, and 0.2% cellulase based on the mass of the lotus leaves was added. The enzymolysis temperature was 35°C and the time was 2 hours. Then, quinoa powder was added (the mass ratio of quinoa powder to lotus leaves was 0.2:1), and then 0.3% neutral protease based on the mass of the lotus leaves was added for enzymolysis at 50°C for 4 hours to obtain an enzymolysis solution.

[0066] 4. Extraction The enzymatic hydrolysate and chrysanthemum solids were mixed in a ratio of 1:1 and added to the extraction tank, and deionized water was added at 50 times the weight of the solids. The extraction temperature was set at 90°C and the extraction time was 1.5 hours. After the extraction was completed, the residue was filtered out with double-layer gauze.

[0067] 5. Freeze clarification The filtrate was rapidly cooled to 4°C and stored for 24 hours to allow the precipitate to precipitate naturally, and the precipitate was filtered out to obtain a clear solution.

[0068] 6. Filtration Filter the above liquid with a common filter.

[0069] 8. Escape from suffering Add 0.6% water-soluble dietary fiber resistant dextrin based on the mass of the clarified liquid to the clarified liquid and stir.

[0070] 9. Sterilization The composite liquid is subjected to high-temperature instantaneous sterilization treatment at a temperature of 137° C. for 30 seconds, thereby preparing a lotus leaf / chrysanthemum composite beverage having lipid-lowering and eye-improving effects.

[0071] The composite drinks obtained in the embodiments and comparative examples were scored according to the following evaluation criteria: Taste: No bitterness, fresh taste (8-10 points); slightly bitter, thin taste (6-7 points); bitter (1-5 points) Stability: uniform color, free of impurities (8-10 points); slightly lighter color, with a little precipitation (6-7 points); contains insoluble substances, easy to precipitate (1-5 points).

[0072] Clinical application examples 80 subjects who had dry eyes, bloodshot eyes, photophobia and tears due to excessive use of eyes were selected and randomly divided into four groups according to ophthalmic symptoms, vision, age and gender factors, with 20 subjects in each group to ensure that the symptoms and vision levels of each group were comparable. The first group took the composite drink prepared in Example 3 for 30 days, the second group took the teabag prepared from only lotus leaf and chrysanthemum powder for 30 days, the third group took the composite drink of comparative example 2 for 30 days, and the fourth group was a blank control group, taking brown sugar water for 30 days.

[0073] Effective: vision is basically restored and eye discomfort symptoms basically disappear; Ineffective means: symptoms are not alleviated.

[0074] The total effective rate of the first group was 90%, the total effective rate of the second group was 50%, the total effective rate of the third group was 70%, and the total effective rate of the fourth group was 5%. The lotus leaf / chrysanthemum composite beverage of the present invention significantly improves eye symptoms.

Claims

1. A method for preparing a lotus leaf / chrysanthemum composite beverage having lipid-lowering and eye-improving effects, characterized in that: The following steps are involved: (1) Lotus leaf pretreatment Choose large, complete, green, spotless fresh lotus leaves, generally lotus leaves that have just grown out of the water for 2 to 3 months are suitable. Use tap water to wash away the mud and impurities on the surface of the lotus leaves, and drain the water; place the lotus leaves in a 100℃ water bath steam for 40-60 seconds; (2) Lotus leaf crushing The pretreated lotus leaves were crushed into small pieces with a size of 0.1 cm*0.1 cm in length; (3) Enzymatic hydrolysis The crushed lotus leaves are added with water at a solid-liquid ratio of 1:5-10, cellulase is added for enzymolysis, quinoa powder is added, and neutral protease is added for enzymolysis to obtain an enzymolysis solution; (4) Extraction The enzymatic hydrolysate and chrysanthemum solids were mixed in a ratio of 1:1 and added to the extraction tank, and deionized water was added at 50-60 times the weight of the solids. The extraction temperature was set at 80-90°C and the extraction time was 1.5h. After the extraction was completed, the residue was filtered out with double-layer gauze. (5) Freezing clarification The filtrate was rapidly cooled to 4°C and stored for 24 hours to allow the precipitate to precipitate naturally, and the precipitate was filtered out to obtain a clear solution; (6) PVDF membrane treatment The above solution was filtered using a 5000 D PVDF nanofiltration membrane; (7) Escape from suffering Add water-soluble dietary fiber resistant dextrin to the above clarified liquid and stir; (8) Sterilization The composite liquid is subjected to high-temperature instantaneous sterilization treatment at a temperature of 137° C. for 30 seconds, thereby preparing a lotus leaf / chrysanthemum composite beverage having lipid-lowering and eye-improving effects.

2. The method for preparing a lotus leaf / chrysanthemum composite beverage having lipid-lowering and eye-improving effects according to claim 1, characterized in that: In step (3), the amount of cellulase added is 0.1%-0.2% of the mass of the lotus leaf, the enzymolysis temperature is 30-50°C, and the time is 1-4 hours.

3. The method for preparing a lotus leaf / chrysanthemum composite beverage having lipid-lowering and eye-improving effects according to claim 1, characterized in that: In step (3), the amount of neutral protease added is 0.1-0.3% of the mass of the lotus leaf, the enzymatic hydrolysis temperature is 30-50°C, and the time is 1-4 hours.

4. The method for preparing a lotus leaf / chrysanthemum composite beverage having lipid-lowering and eye-improving effects according to claim 1, characterized in that: In step (3), the mass ratio of quinoa powder added to lotus leaf is 0.1-0.2:

1.

5. The method for preparing a lotus leaf / chrysanthemum composite beverage having lipid-lowering and eye-improving effects according to claim 1, characterized in that: In step (8), the amount of dextrin added is 0.5-0.8% of the mass of the clarified liquid.

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