Compound nutrition preparation for reducing fat and increasing muscle and preparation method thereof

Abstract

The invention discloses a compound nutritional preparation for reducing fat and increasing muscle and a preparation method thereof, and relates to the field of weight-losing foods. The compound nutrition preparation comprises a fat-reducing and muscle-building composition. The composition comprises the following components: soybean protein isolate, flaxseed powder, whey protein, pumpkin powder, isomaltooligosaccharide, Chinese yam powder, medium-chain fatty acid, calcium beta-hydroxy-beta-methyl butyrate, sunflower seed powder, wheat germ powder, L-glutamine, casein, psyllium husk, konjaku flour, wheat oligopeptide, vitamin E, vitamin D and stevioside. The compound nutritional preparation provided by the invention not only can significantly reduce the weight and the fat content of the whole body, but also can increase the skeletal muscle amount of the whole body, is safe and effective, and has no adverse reaction.

Description

Technical Field

[0001] The invention relates to the field of weight-loss foods, and in particular to a compound nutritional preparation for reducing fat and increasing muscle and a preparation method thereof. Background Art

[0002] With the development of society and changes in lifestyle, obesity has become a health problem that plagues modern people worldwide. Obesity is a risk factor for cardiovascular and cerebrovascular diseases, diabetes, chronic kidney disease, multiple cancers, a series of bone and muscle diseases, and all-cause mortality. The control and prevention of obesity has become a key issue in the field of public health. After people realize the adverse effects of obesity on health, they often instinctively use diet control to lose weight, simply aiming to control their weight. In fact, it is more important to focus on improving body composition during weight loss than simply losing weight. However, most of the current studies on weight loss only focus on dietary energy restriction, and most outcome variables only focus on changes in weight rather than changes in body composition. This also leads to many weight loss methods that simply aim to reduce weight, which is not good for health.

[0003] Given the current severe situation of high obesity prevalence, it is urgent to explore safe and reliable fat reduction strategies. How to maintain skeletal muscle mass and function while reducing fat is a technical problem that needs to be solved urgently.

[0004] Existing weight loss dietary strategies include but are not limited to very low-energy diets, ketogenic diets, high-protein diets, high-fiber diets, time-restricted feeding, etc. The essence of these methods is to limit the intake of energy and specific macronutrients (mainly fat and carbohydrates). Studies have shown that using these methods to lose weight will result in varying degrees of skeletal muscle loss, while the body fat percentage does not decrease significantly. Therefore, most of the weight lost is skeletal muscle rather than fat, which is not good for your health. The weight loss effect is difficult to maintain and the weight is easy to rebound.

[0005] Patent document CN201180007682.3 discloses a nutritional emulsion containing β-hydroxy-β-methylbutyrate (HMB), which is used to maintain and support the lean body mass of elderly people with sarcopenia, provide nutritional support for the elderly and improve muscle strength. It is not used for weight loss in obese people, and its effect on fat loss is unknown; Patent document CN201680060797.1 discloses an HMB compound preparation for enhancing soft tissue repair ability, the purpose of which is to promote wound repair rather than fat loss; Patent document CN201380078730.7 discloses a nutritional formula for maintaining muscle during weight loss, but the formula is centered on protein and vitamin D. However, there are many inventions about improving body fat or enhancing athletic performance, but there are few reports on research on enhancing skeletal muscle performance while reducing fat, and specific enhancement methods and mechanisms are still being explored. Summary of the invention

[0006] The purpose of the present invention is to provide a compound nutritional preparation for reducing fat and increasing muscle and a preparation method thereof, so as to solve the problems existing in the above-mentioned prior art. The compound nutritional preparation provided by the present invention can not only significantly reduce body weight and body fat content, but also increase body skeletal muscle mass, and is safe, effective and has no adverse reactions.

[0007] To achieve the above object, the present invention provides the following solutions: The invention provides a fat-reducing and muscle-increasing composition. The composition comprises the following components by weight: 14-16 parts of soy protein isolate, 7-9 parts of flaxseed powder, 4-6 parts of whey protein, 4-6 parts of pumpkin powder, 4-6 parts of isomaltooligosaccharide, 4.6-5.0 parts of yam powder, 3.8-4.2 parts of medium-chain fatty acids, 0.75-3 parts of calcium beta-hydroxy-beta-methylbutyrate, 2.5-3.5 parts of sunflower seed powder, 2.5-3.5 parts of wheat germ powder, 2.5-3.5 parts of L-glutamine, 1.5-2.5 parts of casein, 1.5-2.5 parts of psyllium husk, 0.8-1.0 parts of konjac powder, 0.8-1.2 parts of wheat oligopeptide, 0.021-0.025 parts of vitamin E, 0.0004-0.0008 parts of vitamin D and 0.04-0.06 parts of stevioside.

[0008] Preferably, the composition comprises the following components by weight: 15 parts of soy protein isolate, 8 parts of flaxseed powder, 5 parts of whey protein, 5 parts of pumpkin powder, 5 parts of isomaltooligosaccharide, 4.6 parts of yam powder, 4.0 parts of medium-chain fatty acids, 3 parts of calcium β-hydroxy-β-methylbutyrate, 3 parts of sunflower seed powder, 3 parts of wheat germ powder, 3 parts of L-glutamine, 2 parts of casein, 2 parts of psyllium husk, 0.9 parts of konjac powder, 1.0 parts of wheat oligopeptides, 0.023 parts of vitamin E, 0.0006 parts of vitamin D and 0.05 parts of steviol glycosides.

[0009] The present invention also provides the use of the above-mentioned composition in preparing a compound nutritional preparation for reducing fat and increasing muscle mass in obese adults.

[0010] The present invention also provides a compound nutritional preparation for reducing fat and increasing muscle mass in obese adults, comprising the above-mentioned composition.

[0011] Furthermore, in order to improve palatability, the compound nutritional preparation also includes seasoning auxiliary materials.

[0012] Furthermore, the seasoning auxiliary material includes cream vanilla essence.

[0013] Furthermore, the dosage form of the compound nutritional preparation includes: powder, gel, liquid, emulsion, capsule or tablet.

[0014] The present invention also provides a method for preparing a compound nutritional preparation for reducing fat and increasing muscle mass in obese adults, comprising: weighing each component according to the formula of the above-mentioned composition, and then mixing them evenly to obtain the compound nutritional preparation.

[0015] The present invention provides a compound nutritional preparation for reducing fat and increasing muscle mass in obese adults. The main ingredients include: soy protein isolate, whey protein, casein, HMB, L-glutamine, wheat oligopeptides, medium-chain fatty acids, vitamin D, vitamin E and konjac flour. The functions of the ingredients are as follows: first, soy protein isolate is a complete protein food additive produced by using low-temperature desolvated soybean meal as raw material. The protein content is above 90%, there are nearly 20 kinds of amino acids, and essential amino acids are contained. It is rich in nutrition and does not contain cholesterol. It is one of the few varieties of plant protein that can replace animal protein. Secondly, HMB is an active metabolite of leucine, and its mechanism of action is: (1) activating the mTOR pathway and increasing IGF-1 expression, thereby increasing muscle protein synthesis; (2) inhibiting the expression and activity of the ubiquitin-protease pathway, thereby inhibiting muscle protein degradation; (3) reducing caspase activity, thereby reducing cell apoptosis in the process of catabolism; (4) reducing systemic chronic inflammation; (5) enhancing muscle satellite cell activity, thereby enhancing muscle regeneration ability. Thirdly, whey protein is a type of protein extracted from milk using modern production technology. It has the advantages of high protein, low fat, easy digestion and absorption, rich in essential amino acids, and high nutritional value. Whey protein contains 20 essential amino acids, as well as branched-chain amino acids such as leucine, isoleucine and valine. It is one of the precursors of glutamate and can provide energy for the body. Sulfur-containing amino acids such as cysteine ​​and methionine have antioxidant functions. The rich threonine can maintain intestinal health. The lysine and arginine contained in it can stimulate the secretion of metabolic hormones and promote muscle growth. Whey protein is a stimulant of cholecystokinin and glucagon-like peptide 1. Cholecystokinin has a regulatory effect on the movement of the small intestine and can regulate satiety. Adding whey protein to the diet can relieve hunger to a certain extent and effectively prolong satiety, which helps to adhere to dietary control for a long time and reduce the intake of food for the next meal. Whey protein also helps to reduce fat synthesis, and has the effect of reducing weight and maintaining muscle mass. Fourth, L-glutamine helps transport carbon and nitrogen in the human body (for cell division and growth), and plays a role in nerve function, intestinal function and strengthening the immune system. L-glutamine can relieve movement syndrome or exercise fatigue, regulate protein synthesis, inhibit protein degradation, glycogen synthesis, cell growth, activate immunity, and increase growth hormone levels. Vitamin D is a fat-soluble vitamin whose main function is to promote the absorption of calcium and phosphorus by small intestinal mucosal cells. In addition, casein is the protein with the highest content in milk. It is currently mainly used as a food raw material or microbial culture medium. Casein phosphopeptides prepared by protein enzymatic hydrolysis technology have multiple physiological effects such as preventing mineral loss, preventing dental caries, preventing and treating osteoporosis and rickets, promoting in vitro fertilization of animals, regulating blood pressure, and treating iron deficiency anemia and magnesium deficiency neuritis.

[0016] In addition, wheat oligopeptides are proteins extracted from natural food wheat protein powder, and then obtained as small molecule polypeptide substances through directional enzyme cutting and specific small peptide separation technology. Wheat oligopeptides can reduce the generation of oxygen free radicals in myocardial mitochondria, maintain the normal morphological structure and function of myocardial mitochondria, improve the ability to tolerate hypoxia, thereby improving the body's endurance and playing a certain role in reducing fatigue. Konjac flour is rich in carbohydrates, low in calories, and has a higher protein content than potatoes and sweet potatoes. It is rich in trace elements and also contains vitamin A, vitamin B, etc., especially glucomannan, which has the effects of weight loss, lowering blood pressure, lowering blood sugar, detoxification and laxative, and eating konjac flour has a significant sense of fullness. Vitamin E has a variety of biological activities and has a preventive and therapeutic effect on some diseases. It is a very strong antioxidant that can protect the stability of cell membranes by interrupting the chain reaction of free radicals, prevent the formation of lipofuscin on the membrane and delay the aging of the body. It can reduce cell oxygen consumption, make people more endurant, and help alleviate leg cramps and stiff hands and feet. Medium-chain fatty acids are a class of compounds composed of three elements: carbon, hydrogen, and oxygen. Medium-chain fatty acids refer to fatty acids with 8-10 carbon atoms in the carbon chain. The use of medium-chain fatty acid oils can reduce blood lipids, cholesterol, and body fat. In addition, it is worth noting that sunflower seeds are rich in linoleic acid and contain estrogen-like compounds such as flavonoids; and flavonoids can promote animal muscle growth through neuroendocrine pathways.

[0017] The present invention discloses the following technical effects: The present invention uses soy protein isolate as the formula basis, and combines other nutrients that increase muscle or promote skeletal muscle protein synthesis (such as sunflower seed powder, L-glutamine, whey protein, HBM, etc.) to develop a compound nutritional preparation, and confirms through a randomized double-blind placebo-controlled trial that the preparation can not only significantly reduce body weight and body fat content, but also increase body skeletal muscle mass, and is safe and effective without adverse reactions.

[0018] The specific advantages of the compound nutritional preparation provided by the present invention are as follows: (1) The present invention utilizes soy protein isolate, flaxseed powder, whey protein, pumpkin powder, oligomaltosaccharide, yam powder, medium-chain fatty acids, calcium β-hydroxy-β-methylbutyrate, sunflower seed powder, wheat germ powder, L-glutamine, casein, psyllium husk, konjac powder, wheat oligopeptide, vitamin E, vitamin D and steviol glycoside for compound preparation, which can effectively block food calorie accumulation, reduce fat accumulation, and reduce body fat content, especially subcutaneous and visceral fat content; in addition, the preparation of the present invention is more sensitive to the effect of reducing fat and increasing muscle in male bodies; (2) The composite preparation of the present invention can effectively promote the growth of skeletal muscle content. Experiments have shown that the formula of the present invention can significantly promote the growth of skeletal muscle fibers and the recovery of muscle fibers after exercise, and has the effect of alleviating muscle fiber atrophy; (3) Sunflower seed powder is added to the composite preparation of the present invention. It is rich in linoleic acid and contains estrogen-like substances, which can promote animal muscle growth through neuroendocrine pathways. It can synergistically promote muscle fiber regeneration with soy protein and promote the transformation of myofilaments to myofibrils, thereby achieving the effect of synergistically promoting muscle growth. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 The figure shows the HE-stained pathological sections of the hind limb skeletal muscles of SD rats fed with the composite preparation of the present invention for 30 days.

[0020] Figure 2 It shows the HE-stained pathological sections of skeletal muscle of the hind limbs of SD rats fed with conventional diet for 30 days.

[0021] Figure 3 It shows the HE-stained pathological sections of the hind limb skeletal muscle of SD rats fed with the composite preparation without sunflower seed powder for 30 days. DETAILED DESCRIPTION

[0022] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0023] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0024] It is intended that the specification and examples be considered exemplary only.

[0025] Example 1 and Comparative Examples 1-5: Mix the formulated amounts of soy protein isolate, flaxseed powder, whey protein, sunflower seed powder, pumpkin powder, isomaltooligosaccharide, yam powder, medium-chain fatty acids, calcium β-hydroxy-β-methylbutyrate, wheat germ powder, L-glutamine, casein, psyllium husk, konjac powder, wheat oligopeptides, vitamin E, vitamin D and steviol glycoside, and pass through a 40-mesh sieve.

[0026] Table 1 Formula of the composite formulation composition prepared in Example 1 and Comparative Examples 1-5 (unit: weight parts):

[0027] Among them, “-” means not added.

[0028] 42 SPF SD rats were prepared and randomly divided into 7 groups according to gender and weight, namely, Example 1 Group (Group I), Comparative Examples 1-5 Groups (Group II-Group VI), and Normal Control Group VII, with 6 rats in each group, half male and half female, and oral gavage. Group I was fed with the formula composition of Example 1, and Groups II-VI were fed with the formula compositions of Comparative Examples 1-5, respectively, with a dose of 4.8 g / kg, a concentration of 0.32 g / mL, and a volume of 15 mL / kg. They were fed once a day for 30 consecutive days; they were fed twice a day, and the Normal Control Group VII Group was fed with conventional feed twice, and Groups I-VII were fed with conventional feed every morning and gavaged with the corresponding formula composition in the afternoon; the rats were weighed on the 7th day and the last day. During the experiment, all groups of rats drank water and exercised normally, and the exercise form was treadmill exercise (model ZH-PT / 5S), with a slope of 0°, a treadmill speed of 20 m / min, and continuous exercise for 30 minutes every day. At the end of the experiment, the animals were euthanized and their body fat was weighed. The body fat included the sum of the rats' visceral fat and subcutaneous fat.

[0029] Table 2 Body weight changes among the groups in Example 1 and Comparative Examples 1-5: * indicates p<0.05 compared with group VII It can be seen that there is no significant difference in the initial weight of each group of rats. After one week of feeding, the weight change difference between the groups is not much. However, after 30 days of feeding, the weight gain of each group of rats slowed down compared with the control group, among which the weight of group I of female rats and group I and group II of male rats showed a significant slowdown in weight gain; further fat content determination found that compared with the control group, the fat content of each group of rats was reduced, among which group I of female rats and groups I, V, and VI of male rats all showed a significant reduction in fat content and a reduced fat accumulation. Male rats seem to be more sensitive to the weight and body fat reduction characteristics of the composition of the present invention.

[0030] Embodiment 2: According to the operation of Example 1, after the animals were euthanized, the experimental animals were further subjected to gross anatomical examination. The gross characteristics were observed with the naked eye, and further dissection was performed on Group I, Group IV, and Group VII: the leg muscles and soleus muscles of the rats were taken, weighed, and frozen; in addition, the skeletal muscle specimens of the hind limbs were placed in a 10% neutral formalin solution, fixed, dehydrated, embedded, routinely paraffin-cut, HE-stained, mounted, and examined under a light microscope.

[0031] Table 3 Relative weight information of leg muscle and soleus muscle of SD rats at the end of feeding period:

[0032] The results showed that compared with group VII, the leg muscle weight of group I rats increased significantly by 36.4%, and the effect was better than that of group IV; the soleus muscle also showed a trend of promotion relative to body weight. Histopathological examination showed that compared with group VII ( Figure 2 ) rat hind limb skeletal muscle fibers showed slight degeneration, group IV ( Figure 3 ) rats also showed slight degeneration of hind limb skeletal muscle fibers, while group I ( Figure 1 ) The skeletal muscle fibers of the rat hind limbs are neatly arranged and compact. This suggests that the soy protein formula composition has the effect of promoting skeletal muscle weight gain, and sunflower seed powder has a synergistic effect with soy protein to promote the recovery and growth of skeletal muscle fibers.

[0033] Embodiment 3: This example is a clinical trial (proving that the effect is better than existing products), and a randomized, single-center, double-blind, placebo-controlled clinical trial is used to evaluate the effect of the composition. The research plan was approved by the Medical Ethics Committee, and all subjects signed informed consent. 30 subjects were recruited and randomly divided into groups, with 10 people in each group. Inclusion criteria: age 30-50 years old, BMI ≥ 28 kg / m 2 ; Able to eat independently, and the amount of food consumed can meet the energy requirements for the whole day; Have the subjective desire to lose weight through calorie restriction, be in good health, and be able to move independently; Be able to communicate normally with researchers; Patients voluntarily participate in this study, promise to cooperate with the completion of relevant intervention measures and examinations, and sign the informed consent form. Those with underlying diseases are excluded.

[0034] The subjects were randomly divided into three groups. The experimental group took the compound nutritional preparation prepared in Example 1 orally (65 g once a day); the control group took a placebo (maltodextrin, 65 g once a day); and the commercially available soy protein powder control group (65 g once a day). The intervention period was 12 weeks. The subjects also received nutritional guidance and performed strength training twice a week. The study began to establish WeChat groups according to the groups to monitor the patients' compliance with oral nutritional preparations and calorie restriction.

[0035] Effect evaluation: The muscle mass and fat percentage were measured using a bioimpedance body composition analyzer (Bailida MC-980MA). The results are shown in Table 4.

[0036] Table 4 Muscle mass and fat percentage measured by bioimpedance body composition analyzer:

[0037] From the data reflected in Table 4, it can be seen that the composition of the present invention can effectively increase the muscle content of the subjects and reduce the body fat rate at the same time, and the effect is better than that of commercially available products.

[0038] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A composition, characterized in that The invention comprises the following components by weight: 14-16 parts of isolated soy protein, 7-9 parts of flaxseed powder, 4-6 parts of whey protein, 4-6 parts of pumpkin powder, 4-6 parts of isomaltooligosaccharide, 4.6-5.0 parts of yam powder, 3.8-4.2 parts of medium-chain fatty acids, 0.75-3 parts of calcium beta-hydroxy-beta-methylbutyrate, 2.5-3.5 parts of sunflower seed powder, 2.5-3.5 parts of wheat germ powder, 2.5-3.5 parts of L-glutamine, 1.5-2.5 parts of casein, 1.5-2.5 parts of psyllium husk, 0.8-1.0 parts of konjac powder, 0.8-1.2 parts of wheat oligopeptide, 0.021-0.025 parts of vitamin E, 0.0004-0.0008 parts of vitamin D and 0.04-0.06 parts of stevioside.

2. The composition according to claim 1, characterized in that The invention comprises the following components by weight: 15 parts of soy protein isolate, 8 parts of flaxseed powder, 5 parts of whey protein, 5 parts of pumpkin powder, 5 parts of isomaltooligosaccharide, 4.6 parts of yam powder, 4.0 parts of medium-chain fatty acids, 3 parts of calcium β-hydroxy-β-methylbutyrate, 3 parts of sunflower seed powder, 3 parts of wheat germ powder, 3 parts of L-glutamine, 2 parts of casein, 2 parts of psyllium husk, 0.9 parts of konjac powder, 1.0 parts of wheat oligopeptide, 0.023 parts of vitamin E, 0.0006 parts of vitamin D and 0.05 parts of stevioside.

3. Use of the composition as claimed in claim 1 or 2 in the preparation of a compound nutritional preparation for fat loss and muscle gain in obese adults.

4. A compound nutritional preparation for reducing fat and increasing muscle mass in overweight / obese adults, characterized in that: Comprising the composition according to claim 1 or 2.

5. The compound nutritional preparation according to claim 4, characterized in that: The compound nutritional preparation also includes seasoning auxiliary materials.

6. The compound nutritional preparation according to claim 5, characterized in that: The seasoning auxiliary material includes cream vanilla essence.

7. The compound nutritional preparation according to claim 5, characterized in that: The dosage form of the compound nutritional preparation includes: powder, gel, liquid, emulsion, capsule or tablet.

8. A method for preparing the compound nutritional preparation according to claim 4, characterized in that: The compound nutritional preparation is obtained by weighing each component according to the formula of the composition of claim 1 or 2 and then mixing them evenly.

9. Use of the composition according to claim 1 or 2 in the preparation of a compound nutritional preparation for increasing skeletal muscle content.

10. Use of the composition according to claim 1 or 2 in the preparation of a compound nutritional preparation for promoting the growth of skeletal muscle fibers.

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