Antioxidant composition and application thereof in cosmetics

Abstract

The invention discloses an antioxidant composition and application thereof in cosmetics. The invention relates to an antioxidant composition, which comprises quercetin and Ectoin, the weight ratio of the quercetin to the ectoine is (1: 0.02)-(1: 400). The antioxidant composition provided by the invention is prepared from quercetin and Ectoin, the weight ratio of the quercetin to the ectoine is (1: 0.02)-(1: 400). The anti-oxidation composition provided by the invention simultaneously contains the quercetin and the Ectoin with a set amount, so that the quercetin and the Ectoin have a good synergistic anti-oxidation effect. The invention further provides application of the composition in preparation of cosmetics, and the content of quercetin in the composition in the cosmetics is 0.01%-1%. According to the application, the composition of quercetin and Ectoine is utilized to generate a relatively good synergistic effect in the aspect of oxidation resistance, so that the quercetin and Ectoine realize an efficient oxidation resistance effect at a relatively low application amount.

Description

Technical Field

[0001] The present invention relates to the field of cosmetic technology, and in particular to an antioxidant composition and its application in cosmetics Background Art

[0002] The skin is a tissue with high metabolic activity, but ultraviolet rays and polluted air in the external environment can induce the skin to produce harmful reactive oxygen species (ROS), which can cause oxidative damage to various biological macromolecules such as cell membranes, lipids, proteins, RNA, DNA, etc., and then destroy cells and increase the chance of DNA damage and mutation. When the reactive oxygen produced by the skin exceeds the capacity of the skin's own antioxidant defense system, the skin will experience oxidative stress.

[0003] Reactive oxygen species (ROS) are secondary metabolites produced by many metabolic pathways in the human body. The human body will continue to produce reactive oxygen species when exposed to ultraviolet light and / or inflammation. Oxidative stress in the skin is caused by an imbalance between oxidants and antioxidants in the skin. If the skin produces too much reactive oxygen species (ROS), or if there are insufficient antioxidants in the skin, or if the antioxidant cell defense system in the skin is reduced, it will cause damage to important processes such as skin cell replication, transcription and translation, leading to rapid skin aging.

[0004] Oxidative stress in the skin is generally caused by skin aging. Antioxidants can terminate the transmission of the oxidation chain in the oxidative stress reaction by combining with free radicals, making it impossible for free radicals to attack the biomacromolecules in the skin cells, thereby reducing the oxidative stress reaction in the skin.

[0005] Although existing antioxidants can alleviate the damage of oxidative stress to the skin to a certain extent, they often require a higher application amount to achieve significant antioxidant effects. This not only increases product costs, but may also cause adverse reactions such as skin irritation and allergies due to excessive use.

[0006] Quercetin has antioxidant effects. However, how to make quercetin achieve efficient antioxidant effects at a lower dosage has become a key technical problem in the research and development of this field. Summary of the invention

[0007] The purpose of the present invention is to provide an antioxidant composition with good antioxidant performance which can be used as an antioxidant active ingredient in cosmetics in view of the deficiencies of the prior art.

[0008] In order to achieve the above object, the present invention provides an antioxidant composition, comprising quercetin and ectoine; the weight ratio of quercetin to ectoine is 1:0.02 to 1:400.

[0009] In some embodiments of the antioxidant composition, the weight ratio of quercetin to ectoine is 1:5 to 1:400.

[0010] In some embodiments of the antioxidant composition, the weight ratio of quercetin to ectoine is 1:10 to 1:40.

[0011] In order to achieve the above-mentioned purpose, the present invention also provides a cosmetic, comprising quercetin and ectoine, wherein the weight ratio of quercetin to ectoine is 1:0.02 to 1:400; the content of the quercetin in the cosmetic is in the range of 0.01 to 1% by weight, and the content of the ectoine in the cosmetic is in the range of 0.01 to 4% by weight.

[0012] In the cosmetics described in some embodiments, the content of quercetin in the cosmetics ranges from 0.1 to 0.5% by weight, and the content of ectoine in the cosmetics ranges from 0.1 to 4% by weight.

[0013] In the cosmetics described in some embodiments, the content of quercetin in the cosmetics is in the range of 0.1-0.5% by weight, and the content of ectoine in the cosmetics is in the range of 0.5-4% by weight.

[0014] In order to achieve the above object, the present invention also provides the use of the antioxidant composition as an antioxidant active ingredient in cosmetics.

[0015] The beneficial effects of the present invention are:

[0016] The antioxidant composition provided by the present invention comprises quercetin and ectoine; the weight ratio of quercetin to ectoine is 1:0.02 to 1:400. The antioxidant composition provided by the present invention comprises a set amount of quercetin and ectoine at the same time, and the two have a good synergistic antioxidant effect, so that the composition has good antioxidant capacity, and can be used as an antioxidant active ingredient in cosmetics to neutralize excessive oxygen free radicals in the skin, thereby delaying the skin aging process. DETAILED DESCRIPTION

[0017] To make the technical solutions and advantages of the present invention more clear, the present invention and its beneficial effects will be further described in detail below in conjunction with specific implementation methods, but the implementation methods of the present invention are not limited thereto.

[0018] In order to achieve the above object, the present invention adopts the following technical solutions:

[0019] An antioxidant composition comprises quercetin and ectoine; the weight ratio of quercetin to ectoine is 1:0.02 to 1:400.

[0020] In some embodiments of the antioxidant composition, the weight ratio of quercetin to ectoine is 1:5 to 1:400.

[0021] In some embodiments of the antioxidant composition, the weight ratio of quercetin to ectoine is 1:10 to 1:40.

[0022] In order to achieve the above-mentioned purpose, the present invention also provides a cosmetic, comprising quercetin and ectoine, wherein the weight ratio of quercetin to ectoine is 1:0.02 to 1:400; the content of the quercetin in the cosmetic is in the range of 0.01 to 1% by weight, and the content of the ectoine in the cosmetic is in the range of 0.01 to 4% by weight.

[0023] In the cosmetics described in some embodiments, the content of quercetin in the cosmetics ranges from 0.1 to 0.5% by weight, and the content of ectoine in the cosmetics ranges from 0.1 to 4% by weight.

[0024] In the cosmetics described in some embodiments, the content of quercetin in the cosmetics is in the range of 0.1-0.5% by weight, and the content of ectoine in the cosmetics is in the range of 0.5-4% by weight.

[0025] In order to achieve the above object, the present invention also provides the use of the antioxidant composition as an antioxidant active ingredient in cosmetics.

[0026] Embodiment 1:

[0027] 0.01 g of quercetin and 0.01 g of ectoine were weighed and mixed evenly to obtain the antioxidant composition of Example 1, and the antioxidant composition was added to 99.98 g of phosphate buffered saline to form a mixed solution, and the mixed solution was heated to completely dissolve the mixed solution at 80°C to obtain an antioxidant composition solution with a quercetin content of 0.01% and an ectoine content of 0.01%.

[0028] The preparation process of the phosphate buffered saline solution is as follows: 8 grams of NaCl, 0.2 grams of KCl, 1.44 grams of Na2HPO4, and 0.24 grams of KH2PO4 are weighed and placed in a 1L container. 800 ml of deionized water is added to the container and stirred for 5 minutes to completely dissolve the components to obtain a mixed solution. The pH value of the mixed solution is measured. If the pH value is not 7.4, Na2HPO4 and / or KH2PO4 are used to fine-tune until the pH value is 7.4. Then deionized water is added to make the volume 1L, and the phosphate buffered saline solution required for Example 1 is obtained.

[0029] The oxidative composition prepared in Example 1 was subjected to an oxidative radical absorbance capacity (ORAC) test.

[0030] The Oxygen Radical Absorbance Capacity (ORAC) test method is one of the most commonly used methods for detecting the antioxidant capacity of antioxidants. The principle is that a free radical initiator such as an azo initiator compound 2,2-azobis(2,-methylpropylimidazole) dihydrochloride is mixed with a fluorescent probe (fluorescein sodium) to destroy the fluorescent probe, causing a change in fluorescence intensity, and the fluorescence decay is quantified by using a fluorometer. The free radical initiator produces peroxy radicals. In the absence of an inhibitor, the free radicals will quickly destroy the fluorescence of the fluorescein dye. By tracking the time course of fluorescence decay and comparing the addition and non-addition of the test substance, the free radical capture capacity of the test substance can be estimated. Trolox, a water-soluble analog of vitamin E, is used as a quantitative standard, i.e., a standard reference substance, and a microplate fluorescence and chemiluminescence analyzer is used for measurement and analysis to measure the relative degree of protection of the sample and the standard reference substance, and the results are expressed in μMol equivalents of the standard reference substance.

[0031] The specific test method is as follows:

[0032] Take 25 μL of the antioxidant composition solution prepared in Example 1 as a sample and add it to a 96-well plate, and take 25 μL of the standard reference substance and add it to a 96-well plate. Add 150 μL of fluorescein solution to the sample and the standard reference substance respectively, and mix the fluorescein solution with the corresponding sample and the standard reference substance; then put the 96-well plate into a 37°C incubator and incubate for 30 minutes. Then add 25 μL of free radical initiator solution to the sample and the standard reference substance respectively and mix them evenly; finally, put the 96-well plate into a microplate fluorescence and chemiluminescence analyzer, and measure the fluorescence intensity of the sample and the standard reference substance under the conditions of an excitation light wavelength of 485 nm, an emission light wavelength of 538 nm, and a measurement temperature of 37°C. The measurement time is 60 min, and the interval is once every 1 min. The 96-well plate, standard reference substance, fluorescein solution, and free radical initiator solution used above were from the ORAC Assay kit (ab233473) of Abcam; the microplate fluorescence and chemiluminescence analyzer used was purchased from ThermoScientific's Fluoroskan FL microplate fluorescence and chemiluminescence analyzer.

[0033] Based on the 60 fluorescence intensity data of the sample measured in chronological order and the 60 fluorescence intensity data of the standard reference substance, the oxygen free radical absorption capacity of the sample and the standard reference substance is calculated. The oxygen free radical absorption capacity calculated in this step is the actual measured value of the oxygen free radical absorption capacity of the sample and the actual measured value of the oxygen free radical absorption capacity of the standard reference substance.

[0034] Table 1 shows the content parameters of each group of antioxidant composition solutions prepared in Examples 1 to 47, the ratio parameters between the components, and the component content parameters of Comparative Examples 1 to 15.

[0035] Table 1:

[0036]

[0037]

[0038]

[0039]

[0040] Examples 2 to 47

[0041] The steps of Example 1 were repeated according to the content parameters of each component and the ratio parameters between the components specified in Examples 2 to 47 in Table 1. See Table 1 for specific parameters and test results.

[0042] Comparative Examples 1 to 15

[0043] The steps of Example 1 were repeated according to the component content parameters specified in Comparative Examples 1 to 15 in Table 1. See Table 1 for specific parameters and test results.

[0044] The measured data of Comparative Examples 1-6 are the actual measured values ​​of the oxygen free radical absorption capacity of the quercetin samples at the corresponding concentrations. The measured data of Comparative Examples 7-15 are the actual measured values ​​of the oxygen free radical absorption capacity of the ectoine samples at the corresponding concentrations.

[0045] The predicted value of the oxygen free radical absorption capacity of the antioxidant composition prepared in Examples 1-47 is: the actual measured value of the oxygen free radical absorption capacity of the quercetin sample of the corresponding concentration in the corresponding composition + the actual measured value of the oxygen free radical absorption capacity of the ectoine sample of the corresponding concentration in the corresponding composition.

[0046] The predicted values ​​and measured values ​​of the oxygen free radical absorption capacity of the antioxidant compositions prepared in Examples 1-47, the measured values ​​of the oxygen free radical absorption capacity of the quercetin samples corresponding to Comparative Examples 1-6, and the measured values ​​of the oxygen free radical absorption capacity of the ectoine samples corresponding to Comparative Examples 7-15 are specifically shown in Table 1.

[0047] From the predicted values ​​and measured values ​​of the oxygen free radical absorption capacity of the corresponding samples of Examples 1-47 shown in Table 1, and the measured values ​​of the oxygen free radical absorption capacity of the corresponding samples of Comparative Examples 1-15, it can be seen that the measured values ​​of the ORAC of the antioxidant composition provided in the embodiments of the present invention are increased by more than 25% compared with the corresponding predicted values, indicating that the active ingredients quercetin and ectoine in the antioxidant composition provided in the embodiments of the present invention have synergistic antioxidant activity. From the specific data in Table 1, it can be seen that the ORAC measured values ​​of most of the antioxidant compositions provided in the embodiments of the present invention are increased by more than 100% compared with the corresponding predicted values. For example, the antioxidant composition solution prepared in Example 13 contains 0.05% quercetin and 0.5% ectoine, and the measured ORAC value obtained by the sample measurement is increased by 1888.35% compared with the predicted value, which indicates that the solution of the antioxidant composition of 0.05% quercetin and 0.5% ectoine provided in Example 13 can produce significant synergistic antioxidant effects. When the antioxidant composition is used as an antioxidant active ingredient in cosmetics, such as containing 0.05% by weight of quercetin and 0.5% by weight of ectoine in the cosmetics, the quercetin and ectoine have the same synergistic antioxidant effect.

[0048] In summary, the antioxidant composition provided by the present invention comprises quercetin and ectoine; the weight ratio of quercetin to ectoine is 1:0.02 to 1:400. The antioxidant composition provided by the present invention comprises a set amount of quercetin and ectoine at the same time, and the two have a good synergistic antioxidant effect, so that the composition has good antioxidant capacity, and can be used as an antioxidant active ingredient in cosmetics to neutralize excessive oxygen free radicals in the skin, thereby delaying the aging process of the skin.

[0049] When the quercetin content and the ectoine content of the cosmetics provided by the present invention are consistent with the content in the antioxidant composition solution prepared by the above embodiment, the ORAC oxygen free radical absorption capacity of the cosmetics and the corresponding antioxidant composition solution is the same, because the ORAC oxygen free radical absorption capacity of the two is synergistically produced by the corresponding content of quercetin and the corresponding content of ectoine, and the solvent in the antioxidant composition solution and other ingredients in the cosmetics have no effect on the antioxidant synergistic effect of quercetin and ectoine. Therefore, the ORAC oxygen free radical absorption capacity of the antioxidant composition solution prepared by the above embodiments 1-47 is equivalent to the ORAC oxygen free radical absorption capacity of the cosmetics containing the corresponding content of quercetin and ectoine, and the corresponding embodiments and corresponding ORAC oxygen free radical absorption capacity test methods and test results are no longer provided for the cosmetics provided by the present invention. Therefore, it can be seen from the oxygen free radical absorption capacity measured in the above Examples 1-47 that when the antioxidant composition of the present application is used as an antioxidant active ingredient in the preparation of cosmetics, the content of quercetin in the cosmetics is 0.01% to 1.%. That is, the composition of quercetin and ectoine used in the present application produces a good synergistic effect in antioxidant effect, so that quercetin and ectoine can achieve efficient antioxidant effect at a lower application amount.

[0050] Those skilled in the art may combine and associate different embodiments or examples and features of different embodiments or examples described in this specification without mutual contradiction.

[0051] The above is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can easily think of various equivalent modifications or replacements within the technical scope disclosed by the present invention, and these modifications or replacements should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention shall be based on the protection scope of the claims.

Claims

1. An antioxidant composition, characterized in that It includes quercetin and ectoine; the weight ratio of quercetin to ectoine is 1:0.02 to 1:

400.

2. The antioxidant composition according to claim 1, characterized in that The weight ratio of quercetin to ectoine is 1:5 to 1:

400.

3. The antioxidant composition according to claim 2, characterized in that The weight ratio of quercetin to ectoine is 1:10 to 1:

40.

4. A cosmetic, characterized in that: The cosmetic comprises quercetin and ectoine, wherein the weight ratio of quercetin to ectoine is 1:0.02 to 1:400; the content of quercetin in the cosmetic is 0.01 to 1% by weight, and the content of ectoine in the cosmetic is 0.01 to 4% by weight.

5. The cosmetic according to claim 4, characterized in that: The content of the quercetin in the cosmetics is in the range of 0.1-0.5% by weight, and the content of the ectoine in the cosmetics is in the range of 0.1-4% by weight.

6. The cosmetic according to claim 5, characterized in that: The content of the quercetin in the cosmetics is in the range of 0.1-0.5% by weight, and the content of the ectoine in the cosmetics is in the range of 0.5-4% by weight.

7. Use of the antioxidant composition according to any one of claims 1 to 3 as an antioxidant active ingredient in cosmetics.

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