Extraction method and application of effective components for improving scalp and hair health

Abstract

The invention provides an extraction method and application of active ingredients for improving scalp and hair health, the extraction method comprises the following steps: S1, crushing and uniformly mixing 25-29 parts by weight of aleppo avens, 12-15 parts by weight of angelica sinensis, 10-13 parts by weight of liquorice and 1-3 parts by weight of ginseng to obtain a mixed traditional Chinese medicine; s2, the mixed traditional Chinese medicine, purified water and a modified lauryl sodium sulfate solution are mixed according to the mass ratio of 1: (9-12): (0.2-0.5), ultrahigh pressure treatment is conducted, the pressure is 280-300 MPa, the pressure maintaining time is 3-5 min, and a mixed treatment solution is obtained; s3, adding the mixed treatment liquid into an extraction tank, heating to 200 + / -5 DEG C, extracting for 40-55 minutes, cooling to below 50 DEG C, filtering, and concentrating filtrate under reduced pressure until the mass of the filtrate is equal to that of the mixed traditional Chinese medicine to obtain a concentrated solution; and S4, adding 3.5-5% of tea polyphenol modified nanoparticles into the concentrated solution, uniformly mixing, dropwise adding organic acid, and adjusting the pH value to 6.3 + / -0.2, thereby obtaining the tea polyphenol modified nano-particles. The prepared effective components are excellent in antibacterial effect, the problems of dandruff and scalp itching can be solved, meanwhile, the strength of hair can be enhanced, and the hair is not prone to breakage.

Description

Technical Field

[0001] The invention belongs to the technical field of plant extraction, and in particular relates to a method for extracting effective ingredients for improving the health of scalp and hair and application thereof. Background Art

[0002] There are many kinds of health problems for scalp and hair. Here are some common problems: 1. Dandruff; Dandruff refers to the flakes of skin that fall off the surface of the scalp, which is usually caused by dry scalp, excessive oil secretion, fungal infection and other reasons. Dandruff not only affects the appearance, but also may cause problems such as scalp itching and inflammation; 2. Hair loss and breakage; Hair loss and breakage will cause the hair to gradually become less, which is usually caused by genetic factors, hormonal imbalance, malnutrition, excessive stress and other reasons. Hair loss and breakage not only affects the appearance, but also may cause psychological problems; 3. Greasy hair: Greasy hair refers to excessive oil on the surface of the hair, which is usually caused by excessive oil secretion of the scalp, incomplete shampooing and other reasons; Greasy hair not only affects the appearance, but also may cause problems such as scalp itching and inflammation; 4. Dry hair; Dry hair refers to the hair losing moisture and oil, becoming dry, fragile and easy to break. Dry hair not only affects the appearance, but also may cause problems such as split ends and breakage.

[0003] Dandruff and scalp itching are the two most common scalp health problems. In addition to excessive dryness of the scalp and improper use of chemical care, which may cause dandruff and scalp itching, fungal infection is one of the common causes of dandruff and scalp itching. The most common scalp disease caused by fungal infection is scalp ringworm, also known as tinea capitis, which usually occurs in children and adolescents. Symptoms of scalp ringworm include red patches, scaling, itching and pain on the scalp. If not treated in time, scalp ringworm may cause hair loss and permanent damage, seriously affecting the health of the scalp and hair. Scalp ringworm is usually caused by fungi of the genus Trichophyton. Among them, Trichophyton rubrum is the most common scalp ringworm pathogen, accounting for more than 90% of scalp ringworm cases.

[0004] There are some antibacterial agents / shampoos for scalp and hair. Although they can achieve good antibacterial effects, thereby treating scalp ringworm, improving dandruff and scalp itching problems, the antibacterial ingredients can also damage the hair, causing it to become brittle and easy to break. Summary of the invention

[0005] In order to solve the problems existing in the background technology, the present invention provides a method for extracting an effective ingredient for improving the health of the scalp and hair and its application. The obtained effective ingredient has excellent antibacterial effect, can improve the problems of dandruff and scalp itching, and can also enhance the strength of the hair, making the hair less likely to break.

[0006] In order to achieve the above objectives, in the first aspect, the present invention provides the following technical solutions: The method for extracting effective ingredients for improving scalp and hair health comprises the following steps: S1. By weight, 25-29 parts of blue basil, 12-15 parts of angelica, 10-13 parts of liquorice and 1-3 parts of ginseng are crushed and mixed evenly to obtain a mixed Chinese medicine; S2, mixing the mixed Chinese medicine obtained in S1 with purified water and modified sodium dodecyl sulfate solution in a mass ratio of 1: (9-12): (0.2-0.5), and subjecting the mixture to ultrahigh pressure treatment at a pressure of 280-300 MPa for a holding time of 3-5 min to obtain a mixed treatment solution; S3, adding the mixed treated liquid obtained in S2 to an extraction tank, heating to 200±5°C, extracting for 40-55 minutes, then cooling to below 50°C, filtering, and concentrating the filtrate under reduced pressure until the mass is equal to that of the mixed Chinese medicine, to obtain a concentrated solution; S4. Add tea polyphenol-modified nanoparticles in an amount of 3.5%-5% by mass to the concentrated solution obtained in S3, mix well, add organic acid dropwise, and adjust the pH value to 6.3±0.2 to obtain effective ingredients for improving the health of scalp and hair.

[0007] In the above technical scheme, Lanbuzheng is the dried whole herb of Rosaceae plants, such as Rosaceae or Rosaceae, which has the effects of invigorating qi and strengthening spleen, nourishing blood and yin; Lanbuzheng can be used to improve scalp and hair, it can promote blood circulation in the scalp, promote nutrient supply to hair follicles, and enhance nutrient supply to hair. In addition, Lanbuzheng can also relieve problems such as dandruff and scalp itching; the main components of Lanbuzheng include volatile oils, flavonoids, triterpenoids, organic acids, sugars, etc., among which volatile oil is one of the main active ingredients of Lanbuzheng, which has antibacterial, anti-inflammatory, antioxidant and other effects, flavonoids have antioxidant, anti-inflammatory, anti-tumor and other effects, triterpenoids have anti-inflammatory, anti-tumor, antiviral and other effects, and organic acids and sugars provide nutrients for Lanbuzheng.

[0008] Angelica is rich in nutrients, such as iron, zinc, vitamin B, etc., which can promote hair growth; the volatile oils and coumarins in angelica can enhance the elasticity and toughness of the hair, promote blood circulation in the scalp, increase the nutrient supply to the hair follicles, and thus improve the health of the scalp; the vitamin E and various amino acids in angelica can protect the hair from damage by free radicals, thereby reducing hair loss.

[0009] Ingredients such as flavonoids and glycyrrhizic acid in licorice have anti-inflammatory and analgesic effects, which can relieve scalp itching and inflammation problems; licorice contains a variety of nutrients, such as iron, zinc, vitamin B, etc., which can promote hair growth; volatile oils and coumarins in licorice can enhance the elasticity and toughness of hair; vitamin E and various amino acids in licorice can protect hair from damage by free radicals, thereby reducing hair loss.

[0010] Ingredients such as ginsenosides in ginseng can dilate blood vessels and promote blood circulation, thereby providing more nutrients and oxygen to the scalp and hair, which helps hair growth; the antioxidant ingredients in ginseng can scavenge free radicals, protect the scalp and hair from oxidative damage, and delay the aging process of hair; ginseng can regulate the body's endocrine system and improve hair problems caused by endocrine disorders, such as hair loss, gray hair, etc.

[0011] Sodium dodecyl sulfate can improve the extraction efficiency in traditional Chinese medicine extraction. However, due to its non-specific adsorption effect on traditional Chinese medicine components, it may also cause the loss of some effective ingredients and affect the efficacy. After experimental verification, it can be avoided that this adverse effect occurs after special modification before being used in traditional Chinese medicine extraction, and even the efficacy of the extract can be further improved. The inventor speculates that this may be because this modification method increases its solubility and stability in water, thereby further improving the extraction efficiency of the combination with traditional Chinese medicine and improving the synergistic performance of efficacy.

[0012] Tea polyphenols are a natural antioxidant with multiple health benefits, including improving the effects on scalp and hair. Specifically, tea polyphenols can neutralize free radicals and reduce oxidative damage, thereby protecting the health of the scalp and hair; tea polyphenols can inhibit inflammatory reactions and alleviate problems such as dandruff and itching; tea polyphenols can promote scalp blood circulation, increase nutrient supply, and contribute to hair growth and health; tea polyphenols can also inhibit the growth of certain fungi and reduce the risk of scalp infection; and it has been verified through experiments that after tea polyphenols are modified by the process of the present invention to prepare tea polyphenol-modified nanoparticles, the ability to improve the efficacy of the drug is further enhanced; The inventors speculate that this may be because the modified nanoparticle state can improve the dispersibility and stability of tea polyphenols and enhance their antioxidant properties; the modified nanoparticle state can improve the permeability and adhesion of tea polyphenols and enhance their antibacterial properties; the tea polyphenol modified nanoparticles can improve their absorption and utilization rate in the body / surface by changing their surface properties and structure; the tea polyphenol modified nanoparticles can also improve the stability and durability of tea polyphenols by encapsulation or adsorption; in short, the experiments verified that after tea polyphenols are made into tea polyphenol modified nanoparticles by the modification process of the present invention, the antibacterial effect of the final product on Trichophyton rubrum and the enhancing effect of the final product on the breaking strength of hair can be further enhanced.

[0013] Furthermore, in S1, 28 parts of blueberry, 13 parts of angelica, 11 parts of liquorice and 2 parts of ginseng are crushed and mixed uniformly by weight to obtain a mixed Chinese medicine.

[0014] Furthermore, the preparation method of the modified sodium dodecyl sulfate solution is as follows: B1. Weigh 100 g of high-purity sodium dodecyl sulfate and place it in a 1000 mL beaker. Add 500 mL of deionized water to the beaker and heat to dissolve at 68 °C to obtain a solution. B2. Add a few drops of concentrated hydrochloric acid to the solution obtained in B1 to adjust the pH value to 7.2, then add 50 g of polyethylene glycol, mix well, and dilute to 1000 mL with deionized water to obtain a modified sodium dodecyl sulfate solution.

[0015] Furthermore, in S2, the mixed Chinese medicine, purified water and modified sodium dodecyl sulfate solution are mixed in a mass ratio of 1:10:0.3.

[0016] Furthermore, the preparation method of the tea polyphenols modified nanoparticles is as follows: A1. Prepare a 2% by mass suspension of waxy corn starch with deionized water, and place it in a 100°C constant temperature water bath with continuous stirring and heating for 40 min to obtain a starch solution; A2, adding 10% tea polyphenols based on the mass of the waxy corn starch to the starch solution obtained in A1, placing the mixture in a 95°C constant temperature water bath and continuously stirring and heating for 20 min, and after the heating is completed, cooling the mixture to 25°C to obtain a mixed solution; A3, dropwise add the mixed solution obtained in A2 into an anhydrous ethanol solution with a volume 5 times that of the mixed solution to precipitate, and after the dropwise addition is completed, a precipitate is obtained; A4. Centrifuge the precipitate obtained in A3 at 3500 r / min for 15 min, recover the supernatant, wash the precipitate three times with anhydrous ethanol solution, recover the alcohol washing solution, and vacuum dry it at -70°C to obtain tea polyphenol modified nanoparticles.

[0017] Furthermore, in S4, tea polyphenol-modified nanoparticles are added to the concentrated solution obtained in S3 at a mass percentage of 4%.

[0018] Furthermore, in S4, the organic acid includes citric acid and / or tartaric acid.

[0019] In the second aspect, the present invention provides the following technical solutions: The application of an effective ingredient for improving the health of scalp and hair, wherein the effective ingredient for improving the health of scalp and hair is prepared by the above-mentioned preparation method and is applied to the preparation of a shampoo with the effect of improving the health of scalp and hair.

[0020] Furthermore, when preparing the shampoo, the mass proportion of the effective ingredients for improving the health of the scalp and hair is 2-3%, and the shampoo stays on the head for 2-5 minutes.

[0021] This application has the following beneficial effects: 1. The mixed Chinese medicinal components of the present invention are indigofera, angelica, liquorice and ginseng. The four Chinese medicinal raw material components are extracted together and work synergistically. They can not only have a good antibacterial effect on Trichophyton rubrum, but also enhance the strength of hair, improve the hair's resistance to stress fracture, and make the hair less likely to break. Moreover, it has been verified by experiments that indigofera is the main medicine and the source of the main functional medicinal ingredients.

[0022] 2. The modified sodium dodecyl sulfate solution is added to the mixed Chinese medicine aqueous solution of the present invention, and then extracted. Compared with adding the unmodified sodium dodecyl sulfate solution, the effective ingredient finally obtained has significantly improved effects on enhancing the hair breaking strength and the antibacterial effect of Trichophyton rubrum.

[0023] 3. The addition of tea polyphenols in the present invention can not only enhance the antibacterial effect of the final product on Trichophyton rubrum, but also enhance the effect of the final product on the strengthening of hair breaking strength. The addition of tea polyphenol-modified nanoparticles obtained by special modification of tea polyphenols can further enhance both of these enhancing abilities. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 The trend diagram of the effect of the active ingredients on the breaking strength of hair obtained from Examples 1-14 and Comparative Examples 1-12 of the present invention; Figure 2 A comparative trend diagram of the minimum inhibitory concentration of the active ingredients obtained in Examples 1-14 of the present invention against Trichophyton rubrum; Figure 3 A comparative trend diagram of the minimum inhibitory concentration of the active ingredients against Trichophyton rubrum obtained in Example 13 of the present invention and Comparative Examples 1-12; Figure 4 This is a comparison chart of the effects of using the active ingredients obtained in Example 13 of the present invention in preparing shampoo. DETAILED DESCRIPTION

[0025] The present application is further described in detail below with reference to the embodiments.

[0026] Unless otherwise specified, the raw materials used in the examples and comparative examples of the present application are all commercially available. Example 1

[0027] The method for extracting effective ingredients for improving scalp and hair health comprises the following steps: S1. By weight, 25 parts of blue basil, 12 parts of angelica, 10 parts of liquorice and 1 part of ginseng were crushed and mixed evenly to obtain a mixed Chinese medicine; S2, mixing the mixed Chinese medicine obtained in S1 with purified water and modified sodium dodecyl sulfate solution in a mass ratio of 1:9:0.2, and subjecting the mixture to ultrahigh pressure treatment at a pressure of 280 MPa and a holding time of 3 min to obtain a mixed treatment solution; S3, adding the mixed treatment liquid obtained in S2 to an extraction tank, heating to 200±5°C, extracting for 45 minutes, then cooling to below 50°C, filtering, and concentrating the filtrate under reduced pressure until the mass is equal to that of the mixed Chinese medicine, to obtain a concentrated solution; S4. Add tea polyphenol-modified nanoparticles at a mass percentage of 3.5% to the concentrated solution obtained in S3, that is, the mass of the tea polyphenol-modified nanoparticles is 3.5% of the mass of the concentrated solution; after uniform mixing, add organic acid dropwise, the organic acid is specifically selected as citric acid, and adjust the pH value to 6.3±0.2 to obtain an effective ingredient for improving the health of the scalp and hair.

[0028] Wherein, the preparation method of modified sodium dodecyl sulfate solution is as follows: B1. Weigh 100 g of high-purity sodium dodecyl sulfate and place it in a 1000 mL beaker. Add 500 mL of deionized water to the beaker and heat to dissolve at 68 °C to obtain a solution. B2. Add a few drops of concentrated hydrochloric acid to the solution obtained in B1 to adjust the pH value to 7.2, then add 50 g of polyethylene glycol, mix well, and dilute to 1000 mL with deionized water to obtain a modified sodium dodecyl sulfate solution.

[0029] The preparation method of tea polyphenols modified nanoparticles is as follows: A1. Prepare a 2% by mass suspension of waxy corn starch with deionized water, and place it in a 100°C constant temperature water bath with continuous stirring and heating for 40 min to obtain a starch solution; A2, adding 10% tea polyphenols based on the mass of the waxy corn starch to the starch solution obtained in A1, placing the mixture in a 95°C constant temperature water bath and continuously stirring and heating for 20 min, and after the heating is completed, cooling the mixture to 25°C to obtain a mixed solution; A3, dropwise add the mixed solution obtained in A2 into an anhydrous ethanol solution with a volume 5 times that of the mixed solution to precipitate, and after the dropwise addition is completed, a precipitate is obtained; A4. Centrifuge the precipitate obtained in A3 at 3500 r / min for 15 min, recover the supernatant, wash the precipitate three times with anhydrous ethanol solution, recover the alcohol washing solution, and vacuum dry it at -70°C to obtain tea polyphenol modified nanoparticles. Example 2

[0030] The difference between this embodiment and embodiment 1 is that in S1, 26 parts of blueberry, 14 parts of angelica, 12 parts of liquorice and 2 parts of ginseng are crushed and mixed uniformly by weight to obtain a mixed Chinese medicine. Example 3

[0031] The difference between this embodiment and embodiment 1 is that in S1, 28 parts of blueberry, 13 parts of angelica, 11 parts of liquorice and 2 parts of ginseng are crushed and mixed uniformly by weight to obtain a mixed Chinese medicine. Example 4

[0032] The difference between this embodiment and embodiment 1 is that in S1, 29 parts of blueberry, 15 parts of angelica, 13 parts of liquorice and 3 parts of ginseng are crushed and mixed uniformly by weight to obtain a mixed Chinese medicine. Example 5

[0033] The only difference between this embodiment and embodiment 3 is that in S2, the mixed Chinese medicine obtained in S1, purified water and modified sodium dodecyl sulfate solution are mixed in a mass ratio of 1:10:0.3 and subjected to ultra-high pressure treatment at a pressure of 290 MPa and a pressure holding time of 4 minutes to obtain a mixed treatment liquid. Example 6

[0034] The only difference between this embodiment and embodiment 3 is that in S2, the mixed Chinese medicine obtained in S1 is mixed with purified water and modified sodium dodecyl sulfate solution in a mass ratio of 1:11:0.4, and ultra-high pressure treatment is performed at a pressure of 280 MPa and a pressure holding time of 5 minutes to obtain a mixed treatment liquid. Example 7

[0035] The only difference between this embodiment and embodiment 3 is that in S2, the mixed Chinese medicine obtained in S1 is mixed with purified water and modified sodium dodecyl sulfate solution in a mass ratio of 1:12:0.5, and ultra-high pressure treatment is performed at a pressure of 300 MPa and a pressure holding time of 5 min to obtain a mixed treatment liquid. Example 8

[0036] The only difference between this embodiment and embodiment 3 is that in S2, the mixed Chinese medicine obtained in S1 is mixed with purified water and modified sodium dodecyl sulfate solution in a mass ratio of 1:10:0.5, and ultra-high pressure treatment is performed at a pressure of 300 MPa and a pressure holding time of 4 minutes to obtain a mixed treatment liquid. Example 9

[0037] The only difference between this embodiment and embodiment 5 is that in S4, tea polyphenol modified nanoparticles are added to the concentrated solution obtained in S3 at a mass percentage of 4%, that is, the mass of the tea polyphenol modified nanoparticles is 4% of the mass of the concentrated solution. Example 10

[0038] The only difference between this embodiment and embodiment 5 is that in S4, tea polyphenol modified nanoparticles are added to the concentrated solution obtained in S3 at a mass percentage of 4.5%, that is, the mass of the tea polyphenol modified nanoparticles is 4.5% of the mass of the concentrated solution. Embodiment 11

[0039] The only difference between this embodiment and embodiment 5 is that in S4, tea polyphenol modified nanoparticles are added to the concentrated solution obtained in S3 at a mass percentage of 5%, that is, the mass of the tea polyphenol modified nanoparticles is 5% of the mass of the concentrated solution. Example 12

[0040] The difference between this embodiment and embodiment 9 is that in S4, the organic acid is specifically selected as tartaric acid. Embodiment 13

[0041] The only difference between this embodiment and embodiment 9 is that in S4, the organic acid includes citric acid and tartaric acid, and the mass ratio of citric acid to tartaric acid is 1:0.5. Embodiment 14

[0042] The only difference between this embodiment and embodiment 9 is that in S4, the organic acid includes citric acid and tartaric acid, and the mass ratio of citric acid to tartaric acid is 1:1.

[0043] Comparative Example 1 The only difference between this comparative example and Example 13 is that ginseng is deleted from the components of the mixed Chinese medicine.

[0044] Specifically, in S1, 28 parts of blue radix, 13 parts of angelica sinensis and 11 parts of liquorice are crushed and mixed uniformly by weight to obtain a mixed Chinese medicine.

[0045] Comparative Example 2 The only difference between this comparative example and Example 13 is that ginseng and liquorice are deleted from the components of the mixed Chinese medicine.

[0046] Specifically, in S1, 28 parts of blue basil and 13 parts of angelica sinensis are crushed and mixed evenly by weight to obtain a mixed Chinese medicine.

[0047] Comparative Example 3 The only difference between this comparative example and Example 13 is that ginseng and angelica are deleted from the components of the mixed Chinese medicine.

[0048] Specifically, in S1, 28 parts of blueberry, 11 parts of liquorice and 2 parts of ginseng are crushed and mixed evenly by weight to obtain a mixed Chinese medicine.

[0049] Comparative Example 4 The difference between this comparative example and Example 13 is that Lanbuzheng is deleted from the components of the mixed Chinese medicine.

[0050] Specifically, in S1, 13 parts of angelica sinensis, 11 parts of liquorice and 2 parts of ginseng are crushed and mixed uniformly by weight to obtain a mixed Chinese medicine.

[0051] Comparative Example 5 The only difference between this comparative example and Example 13 is that the modified sodium dodecyl sulfate solution is replaced by an unmodified sodium dodecyl sulfate solution.

[0052] Specifically, in S2, the mixed Chinese medicine obtained in S1, purified water and sodium dodecyl sulfate solution are mixed in a mass ratio of 1:10:0.3, and subjected to ultra-high pressure treatment at a pressure of 290 MPa and a pressure holding time of 4 minutes to obtain a mixed treatment liquid.

[0053] The preparation method of sodium dodecyl sulfate solution is as follows: weigh 100g of sodium dodecyl sulfate, slowly transfer it to a beaker containing 900mL of water, and stir it with a magnetic stirrer until it is completely dissolved (here, depending on the actual dissolution situation, if simple stirring dissolves slowly, ultrasonic oscillation can be used to help dissolve). Finally, dilute it to 1000mL with water to obtain sodium dodecyl sulfate solution.

[0054] Comparative Example 6 The difference between this comparative example and Example 13 is that the modified sodium dodecyl sulfate solution is deleted.

[0055] Specifically, in S2, the mixed Chinese medicine obtained in S1 and purified water are mixed in a mass ratio of 1:10, and subjected to ultra-high pressure treatment at a pressure of 290 MPa and a pressure holding time of 4 minutes to obtain a mixed treatment liquid.

[0056] Comparative Example 7 The only difference between this comparative example and Example 13 is that the modified sodium dodecyl sulfate solution is replaced by dodecyl dimethyl betaine solution.

[0057] Specifically, in S2, the mixed Chinese medicine obtained in S1, purified water and dodecyl dimethyl betaine solution are mixed in a mass ratio of 1:10:0.3, and subjected to ultra-high pressure treatment at a pressure of 290 MPa and a pressure holding time of 4 minutes to obtain a mixed treatment liquid.

[0058] The preparation method of dodecyl dimethyl betaine solution is as follows: weigh 100g of dodecyl dimethyl betaine, slowly transfer it to a beaker containing 900mL of water, and stir it with a magnetic stirrer until it is completely dissolved (here, according to the actual dissolution situation, if the dissolution is slow by simple stirring, ultrasonic oscillation can be used to help dissolve it). Finally, pour the solution into a volumetric flask, add deionized water, make the volume up to 1000mL with deionized water, cover the volumetric flask with a stopper, and gently shake to mix the solution to obtain the dodecyl dimethyl betaine solution.

[0059] Comparative Example 8 The only difference between this comparative example and Example 13 is that the tea polyphenol-modified nanoparticles are replaced by tea polyphenol.

[0060] Specifically, in S4, tea polyphenols are added to the concentrated solution obtained in S3 in an amount of 4% by mass, that is, the mass of the tea polyphenols is 4% of the mass of the concentrated solution.

[0061] Comparative Example 9 The only difference between this comparative example and Example 13 is that the tea polyphenol-modified nanoparticles are deleted.

[0062] Specifically, S4, adding an organic acid to the concentrated solution obtained in S3, the organic acid including citric acid and tartaric acid, the mass ratio of citric acid to tartaric acid being 1:0.5, and adjusting the pH value to 6.3±0.2 to obtain an effective ingredient for improving the health of the scalp and hair.

[0063] Comparative Example 10 The difference between this comparative example and Example 13 is that the organic acid is specifically selected from sulfinic acid.

[0064] Comparative Example 11 The only difference between this comparative example and Example 13 is that the modified sodium dodecyl sulfate solution is replaced by an unmodified sodium dodecyl sulfate solution; and the tea polyphenol-modified nanoparticles are replaced by tea polyphenols.

[0065] Comparative Example 12 The only difference between this comparative example and Example 13 is that the modified sodium dodecyl sulfate solution and tea polyphenol modified nanoparticles are deleted.

[0066] Test Example 1 Test item: Effect of the active ingredients for improving scalp and hair health of the present invention on the breaking strength of hair.

[0067] Test subjects: the effective ingredients for improving scalp and hair health prepared in Examples 1-14 and Comparative Examples 1-12.

[0068] Test method: Take 28 liquid storage bottles, clean and dry them, and number them 1-28; The liquid storage bottles No. 1-26 are respectively filled with the effective ingredients for improving scalp and hair health prepared in Examples 1-14 and Comparative Examples 1-12; the liquid storage bottle No. 27 is filled with deionized water; the liquid storage bottle No. 28 is left empty as a blank control, thereby creating 28 immersion environments; Take 140 hairs (from the same person) and divide them into 28 groups, with 5 hairs in each group; put the 28 groups of hair into liquid storage bottles numbered 1-28 one by one, soak for 30 minutes, take out all the hair with tweezers, absorb the liquid on the hair with paper towels, and use a spring dynamometer to measure the hair breaking value, that is, the tensile force when breaking (Newton / N), which indicates the maximum tensile force that the hair can withstand, which is an intuitive reflection of the hair's breaking strength; Note: After the test is completed, it is found that the deviation of the test data within each group is not large, and the average value of each group is taken.

[0069] Test results: See Table 1.

[0070] Table 1. Hair breaking strength Result analysis: Based on the data in Table 1 and the Figure 1 It can be seen that the breaking strength of the hair soaked with the active ingredients prepared in Examples 1-14 is significantly higher than that of the blank control, indicating that the active ingredients prepared in the present invention can enhance the strength of the hair, improve the hair's ability to resist force fracture, and make the hair less likely to break; among them, Example 13 is the best example. The breaking strength of the hair No. 27 soaked in deionized water did not increase but decreased, which may be because water can open the scale layer of the hair, making the hair soft, weak and easy to break.

[0071] By combining the data in Table 1 and Figure 1 It can be seen that the four Chinese herbal raw material components in the mixed Chinese medicine are indispensable. The lack of any one of them will reduce the efficacy of enhancing the hair breaking strength. Among them, Lanbuzheng is the main medicine. Once it is deleted, the effect of enhancing the hair breaking strength will drop sharply.

[0072] By combining the data in Table 1 and Figure 1 It can be seen that the test data of Example 13 is significantly greater than the test data of Comparative Example 5, indicating that the addition of modified sodium dodecyl sulfate solution to the mixed Chinese medicine aqueous solution and then extraction, compared with the addition of unmodified sodium dodecyl sulfate solution, the final effective ingredient has a significantly improved enhancement effect on the hair breaking strength; the test data of Comparative Examples 6 and 7 are the same, and both are lower than the test data of Comparative Example 5, indicating that the addition of dodecyl dimethyl betaine solution cannot enhance the breaking strength of the hair, and the dodecyl dimethyl betaine solution cannot replace the efficacy of the sodium dodecyl sulfate solution.

[0073] By combining the data in Table 1 and Figure 1 It can be seen that the effective ingredients prepared in Example 13 and Comparative Examples 8-9 can both enhance the breaking strength of hair, and the enhancing ability of Example 13 is significantly better than that of Comparative Examples 8-9; comparative analysis shows that the addition of tea polyphenols can enhance the enhancing effect of the final product on the breaking strength of hair, and the addition of tea polyphenol-modified nanoparticles obtained by special modification of tea polyphenols can further enhance this enhancing effect.

[0074] By combining the data in Table 1 and Figure 1 It can be seen that the addition of organic acid not only plays a role in adjusting the pH value, but also the effect of replacing citric acid and / or tartaric acid with sulfinic acid on enhancing the hair breaking strength will be reduced.

[0075] By combining the data in Table 1 and Figure 1 It can be seen that the modification treatment method of sodium dodecyl sulfate and tea polyphenols can synergistically enhance the effect of enhancing the breaking strength of hair, and it is not a simple superposition of two enhancing effects.

[0076] Test Example 2 Test item: Minimum inhibitory concentration (MIC) against Trichophyton rubrum.

[0077] Test subjects: the effective ingredients for improving scalp and hair health prepared in Examples 1-14 and Comparative Examples 1-12.

[0078] Things to note during the test process: The MIC value of each group is the hole with the lowest drug concentration in the test hole where no bacteria grow, that is, the minimum inhibitory concentration is obtained; the antibacterial experiment of each test object is carried out twice in parallel, and in the observation of the parallel experimental results, the two MIC values ​​are acceptable only when they can be accurately repeated or differ by only one concentration; if the two MIC values ​​differ by more than two concentrations, the experiment needs to be repeated until it meets the requirements.

[0079] Test results: See Table 2.

[0080] Table 2. Minimum inhibitory concentrations against Trichophyton rubrum Result analysis: Based on the data in Table 1 and the Figure 2 It can be seen that the effective ingredients prepared in Examples 1-14 all have good antibacterial effects on Trichophyton rubrum, among which Example 13 is the best.

[0081] By combining the data in Table 1 and Figure 3It can be seen that the active ingredients prepared in Example 13 and Comparative Examples 1-4 have a certain antibacterial effect on Trichophyton rubrum, and the antibacterial effect of the active ingredient prepared in Example 13 on Trichophyton rubrum is significantly better than that of Comparative Examples 1-4; comparative analysis shows that the lack of any one of the four Chinese medicine raw material components in the mixed Chinese medicine will reduce the antibacterial effect on Trichophyton rubrum.

[0082] By combining the data in Table 1 and Figure 3 It can be seen that the effective ingredients prepared in Example 13 and Comparative Examples 5-7 have a certain antibacterial effect on Trichophyton rubrum, and the antibacterial effect of the effective ingredient prepared in Example 13 on Trichophyton rubrum is significantly better than that of Comparative Examples 5-7; comparative analysis shows that adding modified sodium dodecyl sulfate solution to the mixed Chinese medicine aqueous solution and then extracting it, compared with adding unmodified sodium dodecyl sulfate solution, the final effective ingredient has a significantly improved enhancing effect on the antibacterial effect of Trichophyton rubrum.

[0083] By combining the data in Table 1 and Figure 3 It can be seen that the active ingredients prepared in Example 13 and Comparative Examples 8-9 have a certain antibacterial effect on Trichophyton rubrum, and the antibacterial effect of the active ingredient prepared in Example 13 on Trichophyton rubrum is significantly better than that of Comparative Examples 8-9; comparative analysis shows that the addition of tea polyphenols can enhance the antibacterial effect of the final product on Trichophyton rubrum, and the addition of tea polyphenol-modified nanoparticles obtained by special modification of tea polyphenols significantly enhances the enhancement effect.

[0084] By combining the data in Table 1 and Figure 3 It can be seen that the addition of organic acid is not only used to adjust the pH value. The preferred citric acid and / or tartaric acid are more conducive to improving the antibacterial effect on Trichophyton rubrum than sulfinic acid. The inventors speculate that this may be because citric acid and tartaric acid themselves have better antibacterial ability against Trichophyton rubrum than sulfinic acid, or it may be because citric acid, tartaric acid and other components work together to exert a better antibacterial effect.

[0085] By combining the data in Table 1 and Figure 3 It can be seen that the modification treatment method of sodium dodecyl sulfate and tea polyphenols can synergistically enhance the antibacterial effect against Trichophyton rubrum.

[0086] Test Example 3 Test project: Effect of application in the preparation of shampoo.

[0087] Test subject: Example 13.

[0088] Test method: The effect of applying the active ingredient prepared in Example 13 of the present invention to shampoo was tested; Specifically, three commercially available affordable shampoos (specific brands and models are omitted here) were selected as blank groups; the active ingredients prepared in Example 13 were added to the corresponding shampoo blank groups at a mass ratio of 2.6% to form shampoo test groups; Each group consisted of 30 people, including 15 males and 15 females, aged 20-50 years old, all of whom had various symptoms of unhealthy scalp and hair, such as itchy scalp and dandruff; The corresponding group samples were used twice a week, and 15-60 mL each time was used according to the thickness of the hair. The experimental period was two months. Specifically, the shampoo was kept on the head for 3 minutes. The efficacy standards are: 1. Significantly effective; the symptoms of itchy scalp, excessive dandruff or oily scalp are significantly improved; 2. Effective; the symptoms of itchy scalp, excessive dandruff or oily scalp are slightly reduced; 3. Ineffective; there is no change before and after use.

[0089] Test results: See Table 3.

[0090] Table 3. Effects of application in shampoo / conditioner Result analysis: Combined with the data in Table 3 Figure 4 It can be seen that the effective ingredients prepared in Example 13 of the present invention can be directly used to prepare shampoo by physical addition, and can effectively relieve itching and remove dandruff, and can significantly improve the anti-itching and anti-dandruff effects of ordinary commercially available shampoos, and the anti-itching and anti-dandruff efficiency can be increased to more than 85%.

[0091] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

[0092] In addition, various embodiments of the present invention may be arbitrarily combined, and as long as they do not violate the concept of the present invention, they should also be regarded as the contents disclosed by the present invention.

Claims

1. A method for extracting an effective ingredient for improving the health of scalp and hair, characterized in that: The steps include: S1. By weight, 25-29 parts of blue basil, 12-15 parts of angelica, 10-13 parts of liquorice and 1-3 parts of ginseng are crushed and mixed evenly to obtain a mixed Chinese medicine; S2, mixing the mixed Chinese medicine obtained in S1 with purified water and modified sodium dodecyl sulfate solution in a mass ratio of 1: (9-12): (0.2-0.5), and subjecting the mixture to ultrahigh pressure treatment at a pressure of 280-300 MPa for a holding time of 3-5 min to obtain a mixed treatment solution; S3, adding the mixed treatment liquid obtained in S2 to an extraction tank, heating it to 200±5°C, extracting it for 40-55 minutes, then cooling it to below 50°C, filtering it, and concentrating the filtrate under reduced pressure until the mass is equal to the mass of the mixed extract, to obtain a concentrated solution; S4. Add tea polyphenol-modified nanoparticles in an amount of 3.5%-5% by mass to the concentrated solution obtained in S3, mix well, add organic acid dropwise, and adjust the pH value to 6.3±0.2 to obtain effective ingredients for improving the health of scalp and hair.

2. The method for extracting effective ingredients for improving scalp and hair health according to claim 1, characterized in that: In S1, 28 parts of blueberry, 13 parts of angelica, 11 parts of liquorice and 2 parts of ginseng are crushed and mixed uniformly by weight to obtain a mixed Chinese medicine.

3. The method for extracting effective ingredients for improving scalp and hair health according to claim 1, characterized in that: The preparation method of the modified sodium dodecyl sulfate solution is as follows: B1. Weigh 100 g of high-purity sodium dodecyl sulfate and place it in a 1000 mL beaker. Add 500 mL of deionized water to the beaker and heat to dissolve at 68 °C to obtain a solution. B2. Add a few drops of concentrated hydrochloric acid to the solution obtained in B1 to adjust the pH value to 7.2, then add 50 g of polyethylene glycol, mix well, and dilute to 1000 mL with deionized water to obtain a modified sodium dodecyl sulfate solution.

4. The method for extracting an effective ingredient for improving scalp and hair health according to claim 1, 2 or 3, characterized in that: In S2, the mixed Chinese medicine, purified water and modified sodium dodecyl sulfate solution are mixed in a mass ratio of 1:10:0.

3.

5. The method for extracting effective ingredients for improving scalp and hair health according to claim 1, characterized in that: The preparation method of the tea polyphenol modified nanoparticles is as follows: A1. Prepare a 2% by mass suspension of waxy corn starch with deionized water, and place it in a 100°C constant temperature water bath with continuous stirring and heating for 40 min to obtain a starch solution; A2, adding 10% tea polyphenols based on the mass of the waxy corn starch to the starch solution obtained in A1, placing the mixture in a 95°C constant temperature water bath and continuously stirring and heating for 20 min, and after the heating is completed, cooling the mixture to 25°C to obtain a mixed solution; A3, dropwise add the mixed solution obtained in A2 into an anhydrous ethanol solution with a volume 5 times that of the mixed solution to precipitate, and after the dropwise addition is completed, a precipitate is obtained; A4. Centrifuge the precipitate obtained in A3 at 3500 r / min for 15 min, recover the supernatant, wash the precipitate three times with anhydrous ethanol solution, recover the alcohol washing solution, and vacuum dry it at -70°C to obtain tea polyphenol modified nanoparticles.

6. The method for extracting effective ingredients for improving scalp and hair health according to claim 1 or 5, characterized in that: In S4, tea polyphenol-modified nanoparticles are added to the concentrated solution obtained in S3 at a mass percentage of 4%.

7. The method for extracting effective ingredients for improving scalp and hair health according to claim 1, characterized in that: In S4, the organic acid includes citric acid and / or tartaric acid.

8. The use of active ingredients for improving scalp and hair health, characterized in that: The effective ingredient for improving the health of the scalp and hair is prepared by the extraction method according to any one of claims 1 to 7, and is used in preparing a shampoo with the effect of improving the health of the scalp and hair.

9. The use of an effective ingredient for improving scalp and hair health according to claim 8, characterized in that: When preparing the shampoo cream, the mass proportion of the effective ingredients for improving the health of the scalp and hair is 2.5-3%, and the shampoo cream stays on the head for 2-5 minutes.

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