Anti-wrinkle, whitening and moisturizing lotion containing conopeptide and preparation process of anti-wrinkle, whitening and moisturizing lotion

Abstract

The invention provides an anti-wrinkle, whitening and moisturizing lotion containing conopeptide and a preparation process thereof, and belongs to the technical field of cosmetics. The preparation method comprises the following steps: preparing the litchi peel extract; preparing a spreading hedyotis herb extract and a pseudo-ginseng root extract; preparing a waste beer yeast extract; and mixing to prepare the anti-wrinkle, whitening and moisturizing lotion. The raw materials adopted by the prepared anti-wrinkle whitening and moisturizing lotion are all natural components, high in safety, mild and non-irritating, conopeptide is dissolved in deionized water and evenly mixed with the pseudo-ginseng root extract to prepare a mixed water phase, then the mixed water phase is added, and the anti-wrinkle whitening and moisturizing lotion is prepared through high-speed dispersion and high-pressure nanometer homogenizer treatment. The conopeptide and the pseudo-ginseng root extract are combined for use, so that skin aging can be more comprehensively and effectively delayed, and the depth and the breadth of wrinkles are reduced.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to an anti-wrinkle, whitening and moisturizing emulsion containing cono peptide and a preparation process thereof. Background Art

[0002] With the improvement of people's living standards and the increasing attention to their own image, skin care and maintenance has become an important research and consumption field. Skin aging is a complex physiological process, which is mainly affected by the combined influence of internal factors (such as age, endocrine changes, genetics, etc.) and external factors (such as ultraviolet radiation, environmental pollution, life pressure, etc.). Among them, the formation of wrinkles is one of the most obvious external manifestations of skin aging. It not only makes the skin lose elasticity and luster, but also seriously affects the individual's appearance and self-confidence. At the same time, whitening is also one of the skin care goals pursued by many consumers. Excessive skin pigmentation can lead to dull skin tone, spots and other problems. These problems are closely related to factors such as ultraviolet-induced increased melanin synthesis, abnormal melanocyte function and slowed skin metabolism. In addition, the moisturizing function of the skin is also crucial to maintaining its health and appearance. Normal skin water content can keep the skin soft, smooth and elastic, and prevent the occurrence of dry, rough and flaky skin.

[0003] At present, the effects of common anti-wrinkle, whitening and moisturizing products on the market are poor, and most of them use chemical ingredients, which leads to greater irritation and dependence of the products and reduced safety. Although traditional anti-wrinkle ingredients such as retinol (such as vitamin A alcohol) have certain anti-wrinkle effects, they are more irritating and easily cause adverse reactions such as skin redness, itching, and peeling, which limits their application in some people with sensitive skin. Although common whitening ingredients (such as arbutin, niacinamide, etc.) are relatively safe, some consumers may be intolerant to these ingredients. For example, after using niacinamide, the skin will become red and stinging, which is "niacinamide intolerance". In addition, traditional moisturizers such as glycerin and sodium hyaluronate mainly reduce water loss by forming a moisturizing film on the skin surface, but they have limited effect on improving the moisturizing mechanism inside the skin and enhancing the skin's own moisturizing ability.

[0004] Therefore, it is necessary to propose an anti-wrinkle, whitening and moisturizing lotion containing cono peptides with high safety, comprehensive efficacy and significant effect and a preparation process thereof. Summary of the invention

[0005] In view of the shortcomings of the prior art, the object of the present invention is to provide an anti-wrinkle, whitening and moisturizing emulsion containing cono peptide and a preparation process thereof.

[0006] A preparation process of an anti-wrinkle, whitening and moisturizing emulsion containing cono peptides comprises the following steps: S1: Preparation of litchi peel extract The litchi peel is washed, dried and ground, and then stirred and mixed with an ethanol solution, and subjected to ultrasonic-assisted heating reflux extraction. After repeated extraction, the extracts are combined and concentrated under reduced pressure to obtain a litchi peel extract; S2: Preparation of Hedyotis diffusa extract and Panax notoginseng root extract Grinding Hedyotis diffusa and Panax notoginseng root into powder respectively and adding them into ethanol solution for heating and reflux extraction, and concentrating under reduced pressure to obtain Hedyotis diffusa extract and Panax notoginseng root extract; S3: Preparation of spent brewer's yeast extract Adding waste beer yeast into an ethanol solution, performing heating reflux extraction, recovering the supernatant after centrifugation, and concentrating under reduced pressure to obtain a waste beer yeast extract; S4: Mixing to prepare anti-wrinkle whitening moisturizing lotion First, rosa damascena flower oil and soybean oil are mixed to prepare a mixed oil phase, then cono peptide is dissolved in deionized water, and the above-mentioned litchi peel extract, oldenlandia diffusa extract, Panax notoginseng root extract and brewer's waste yeast extract are added to prepare a mixed water phase, and then the mixed oil phase is mixed with the mixed water phase to obtain an anti-wrinkle, whitening and moisturizing lotion.

[0007] Furthermore, S1 specifically includes the following steps: S1.1: After washing and drying the litchi peel, add it into a grinder and grind it thoroughly to obtain litchi peel powder; S1.2: Add the above litchi peel powder to 80% ethanol solution at a material-liquid ratio of 1 g: (30-40) mL, stir and mix evenly, heat and reflux at 60-70°C and 100-200 W ultrasonic power for 1-2 h, and filter to obtain a primary litchi peel extract and a primary litchi peel residue; S1.3: Add the above litchi peel filter residue to 80% ethanol solution at a solid-liquid ratio of 1g: (20-30)mL, heat and reflux at 60-70℃ and 100-150W ultrasonic power for extraction for 1-2h. After filtration, combine the filtrate with the above litchi peel extract, and concentrate under reduced pressure at 40-50℃ to dryness to obtain litchi peel extract.

[0008] Furthermore, S2 specifically includes the following steps: S2.1: After washing, drying and grinding Hedyotis diffusa into powder, add it into 75% ethanol solution at a solid-liquid ratio of 1:(30-40) mL, heat and stir under reflux extraction at 70-80°C for 1-2h, and filter under reduced pressure to obtain a primary Hedyotis diffusa extract and a primary Hedyotis diffusa residue; S2.2: Add the above-mentioned first Hedyotis diffusa filter residue to 75% ethanol solution at a solid-liquid ratio of 1 g: (30-40) mL, heat and stir under reflux at 70-80°C for 1-2 h, filter under reduced pressure, combine the filtrate with the above-mentioned first Hedyotis diffusa extract, and concentrate under reduced pressure at 40-50°C to dryness to obtain a Hedyotis diffusa extract; S2.3: Dry and grind the root of Panax notoginseng into powder, and add it into 60% ethanol solution at a solid-liquid ratio of 1g:(40-50)mL. Heat and stir under reflux extraction at 55-65℃ for 40-50min, repeat the extraction twice, combine the filtrate, and concentrate under reduced pressure at 50-60℃ to dryness to obtain the Panax notoginseng root extract.

[0009] Furthermore, S3 specifically includes the following steps: S3.1: Add the waste brewer's yeast into 80% ethanol solution at a solid-liquid ratio of 1g: (20-30)mL, heat and reflux at 50-60℃ for 1-2h to obtain an extract; S3.2: The above extract is transferred to a centrifuge, centrifuged at a rate of 4000-5000 r / min for 10-20 min, the supernatant is recovered, and the supernatant is concentrated under reduced pressure at 30-40° C. to dryness to obtain a waste beer yeast extract.

[0010] Furthermore, S4 specifically includes the following steps: S4.1: stirring and mixing the Rosa damascena flower oil and soybean oil to obtain a mixed oil phase; S4.2: Add cono peptide to deionized water, stir thoroughly to dissolve, then add litchi peel extract obtained in step S1.3, Hedyotis diffusa extract obtained in step S2.2, notoginseng root extract obtained in step S2.3 and brewer's waste yeast extract obtained in step S3.2, stir thoroughly to mix, and obtain a mixed aqueous phase; S4.3: Add the above mixed oil phase into the above mixed water phase, mix for 3-5 minutes using a high-speed disperser, and then process using a high-pressure nano homogenizer to obtain an anti-wrinkle, whitening and moisturizing lotion.

[0011] Furthermore, the anti-wrinkle, whitening and moisturizing lotion comprises, by weight: 2-3 parts of cono peptide, 8-10 parts of litchi peel extract, 3-5 parts of oldenlandia diffusa extract, 3-5 parts of Panax notoginseng root extract, 10-12 parts of brewer's waste yeast extract, 2-3 parts of Rosa damascena flower oil, 12-14 parts of soybean oil and 100-120 parts of deionized water.

[0012] Furthermore, the speed of the high-speed disperser is 10000-12000 r / min.

[0013] Furthermore, the processing pressure of the high-pressure nano-homogenizer is 90-100 MPa, and the number of processing times is 2 times.

[0014] An anti-wrinkle, whitening and moisturizing emulsion containing cono peptide is prepared by any of the above-mentioned preparation processes for the anti-wrinkle, whitening and moisturizing emulsion containing cono peptide.

[0015] Compared with the prior art, the present invention has the following beneficial effects: 1. The raw materials used in the anti-wrinkle, whitening and moisturizing lotion prepared by the present invention are all natural ingredients, which are highly safe, mild and non-irritating. The root of Panax notoginseng is first extracted with an ethanol solution to obtain a Panax notoginseng root extract, and then cono peptide is dissolved in deionized water and uniformly mixed with the Panax notoginseng root extract to form a mixed aqueous phase. Finally, the mixed oil phase is added to the mixed aqueous phase, and the anti-wrinkle, whitening and moisturizing lotion is prepared by high-speed dispersion and high-pressure nano homogenizer treatment. Among them, since cono peptide directly activates fibroblasts to secrete collagen, the Panax notoginseng root extract provides more nutrition and oxygen to fibroblasts by improving blood circulation, promoting their proliferation. The combination of the two can enhance the synthesis effect of collagen. Moreover, while cono peptide inhibits neuromuscular contraction and reduces wrinkle formation, the Panax notoginseng root extract scavenges free radicals to prevent the skin extracellular matrix from being destroyed. Therefore, the combination of cono peptide and Panax notoginseng root extract can more comprehensively and effectively delay skin aging and reduce the depth and breadth of wrinkles.

[0016] 2. The present invention firstly extracts litchi peel and Hedyotis diffusa with ethanol solutions of different concentrations to obtain litchi peel extract and Hedyotis diffusa extract, then adds the litchi peel extract and Hedyotis diffusa extract into a mixed aqueous phase, and mixes them with a mixed oil phase to prepare an emulsion. Since the moisturizing film formed by the litchi peel extract can directly prevent water loss, while the Hedyotis diffusa extract can protect cells from free radical damage while maintaining the normal function of skin cells, thereby ensuring the health of the skin stratum corneum and helping to strengthen the moisturizing film formed by the litchi peel extract, when the skin is affected by external factors (such as ultraviolet rays, pollution, etc.), resulting in slight damage to the moisturizing film or damage to skin cells, the Hedyotis diffusa extract can play its repairing role, so that the moisturizing film can better play its moisturizing function, so the two have a synergistic moisturizing effect. When the litchi peel extract and the Hedyotis diffusa extract are used to prepare the emulsion at the same time, the moisturizing effect of the emulsion can be further enhanced.

[0017] 3. When the oldenlandia diffusa extract and the brewer's yeast extract are added to the mixed aqueous phase for preparing the emulsion, the oldenlandia diffusa extract reduces the production of melanin by inhibiting the activity of tyrosinase and scavenging free radicals. After the oldenlandia diffusa extract takes effect, the brewer's yeast extract promotes the metabolism of skin cells, and the accelerated metabolism can more quickly remove the free radicals around the melanocytes, further enhancing the inhibitory effect of the oldenlandia diffusa extract on tyrosinase. Therefore, when the two are used in combination, it is beneficial to improve the overall whitening effect.

[0018] 4. After the present invention mixes the druskea rose flower oil and soybean oil as the oil phase, on the one hand, since cono peptides can regulate the activity of melanocytes and inhibit the synthesis and transport of melanin, and the vitamin C in the druskea rose flower oil prevents the formation of melanin from the perspective of chemical reduction, the combination of the two can more effectively reduce the synthesis of melanin. At the same time, the antioxidant components in the druskea rose flower oil can remove free radicals, reduce the stimulation of free radicals on melanocytes, and further inhibit the excessive production of melanin, thereby further enhancing the whitening effect of the emulsion. On the other hand, since cono peptides can promote the synthesis of natural moisturizing factors in skin cells and enhance the moisturizing ability of the skin stratum corneum, and the fatty acids in the druskea rose flower oil can supplement skin lipids, form a protective film on the skin surface, and reduce the loss of skin moisture, these two mechanisms complement each other, respectively enhancing internal moisturizing and preventing external moisture loss, thereby further improving the moisturizing effect of the emulsion. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The accompanying drawings, which are incorporated herein and constitute a part of the specification, illustrate embodiments of the present disclosure and, together with the description, further serve to explain the principles of the present disclosure and to enable those skilled in the relevant art to make and use the present disclosure.

[0020] Figure 1 This is a process flow chart for preparing the anti-wrinkle, whitening and moisturizing lotion containing cono peptides used in the embodiments of the present invention. DETAILED DESCRIPTION

[0021] The following is a detailed description of an anti-wrinkle, whitening and moisturizing lotion containing cono peptides and its preparation process provided by the present invention in conjunction with specific embodiments. At the same time, it is explained here that in order to make the embodiments more detailed, the following embodiments are the best and preferred embodiments, and those skilled in the art may also adopt other alternatives to implement some known technologies.

[0022] Example 1 A preparation process of an anti-wrinkle, whitening and moisturizing lotion containing cono peptide, such as Figure 1 As shown, the following steps are included: S1: Preparation of litchi peel extract S1.1: After washing and drying the litchi peel, add it into a grinder and grind it thoroughly to obtain litchi peel powder; S1.2: Add the above litchi peel powder to 80% ethanol solution at a solid-liquid ratio of 1g:30mL, stir and mix evenly, heat and reflux at 60°C and 100W ultrasonic power for 1h, and filter to obtain a primary litchi peel extract and a primary litchi peel residue; S1.3: Add the above litchi peel residue to 80% ethanol solution at a solid-liquid ratio of 1g:20mL, heat and reflux at 60°C and 100W ultrasonic power for extraction for 1h, filter, combine the filtrate with the above litchi peel extract, and concentrate under reduced pressure at 40°C to dryness to obtain litchi peel extract; S2: Preparation of Hedyotis diffusa extract and Panax notoginseng root extract S2.1: After washing, drying and grinding Hedyotis diffusa into powder, add it into 75% ethanol solution at a solid-liquid ratio of 1:30 mL, heat and stir under reflux at 70°C for 1 h, and filter under reduced pressure to obtain a primary Hedyotis diffusa extract and a primary Hedyotis diffusa residue; S2.2: Add the above-mentioned first Hedyotis diffusa filter residue to 75% ethanol solution at a solid-liquid ratio of 1g:30mL, heat and stir under reflux at 70°C for 1h, filter under reduced pressure, combine the filtrate with the above-mentioned first Hedyotis diffusa extract, and concentrate under reduced pressure at 40°C to dryness to obtain a Hedyotis diffusa extract; S2.3: Dry and grind the root of Panax notoginseng into powder, add it into 60% ethanol solution at a solid-liquid ratio of 1g:40mL, heat and stir under reflux extraction at 55°C for 40min, repeat the extraction twice, combine the filtrates, concentrate under reduced pressure at 50°C to dryness, and obtain the Panax notoginseng root extract; S3: Preparation of spent brewer's yeast extract S3.1: Add the waste brewer's yeast into 80% ethanol solution at a solid-liquid ratio of 1g:20mL, heat and reflux at 50℃ for 1h to obtain an extract; S3.2: The extract is transferred to a centrifuge, centrifuged at 4000 r / min for 10 min, the supernatant is recovered, and the supernatant is concentrated under reduced pressure at 30° C. to dryness to obtain a spent beer yeast extract; S4: Mixing to prepare anti-wrinkle whitening moisturizing lotion S4.1: 2 parts by weight of Rosa damascena flower oil and 12 parts by weight of soybean oil were stirred and mixed to obtain a mixed oil phase; S4.2: Add 2 parts by weight of cono peptide to 100 parts by weight of deionized water, stir thoroughly to dissolve, then add 8 parts by weight of litchi peel extract obtained in step S1.3, 3 parts by weight of Hedyotis diffusa extract obtained in step S2.2, 3 parts by weight of Panax notoginseng root extract obtained in step S2.3 and 10 parts by weight of waste brewer's yeast extract obtained in step S3.2, stir thoroughly to mix, and obtain a mixed aqueous phase; S4.3: Add the above mixed oil phase to the above mixed water phase, mix at a rate of 10000 r / min for 3 min using a high-speed disperser, and then treat twice at a pressure of 90 MPa using a high-pressure nano homogenizer to obtain an anti-wrinkle, whitening and moisturizing lotion.

[0023] Example 2 A preparation process of an anti-wrinkle, whitening and moisturizing lotion containing cono peptide, such as Figure 1 As shown, the following steps are included: S1: Preparation of litchi peel extract S1.1: After washing and drying the litchi peel, add it into a grinder and grind it thoroughly to obtain litchi peel powder; S1.2: Add the above litchi peel powder to 80% ethanol solution at a material-liquid ratio of 1g:35mL, stir and mix evenly, heat and reflux for extraction at 65°C and 150W ultrasonic power for 1.5h, and filter to obtain a primary litchi peel extract and a primary litchi peel residue; S1.3: Add the above litchi peel residue to 80% ethanol solution at a solid-liquid ratio of 1g:25mL, heat and reflux at 65°C and 125W ultrasonic power for 1.5h, filter, combine the filtrate with the above litchi peel extract, and concentrate under reduced pressure at 45°C to dryness to obtain litchi peel extract; S2: Preparation of Hedyotis diffusa extract and Panax notoginseng root extract S2.1: After washing, drying and grinding Hedyotis diffusa into powder, add it into 75% ethanol solution at a solid-liquid ratio of 1:35 mL, heat and stir under reflux at 75°C for 1.5 h, and filter under reduced pressure to obtain a primary Hedyotis diffusa extract and a primary Hedyotis diffusa residue; S2.2: Add the above-mentioned Hedyotis diffusa filter residue to 75% ethanol solution at a solid-liquid ratio of 1g:35mL, heat and stir under reflux at 75°C for 1.5h, filter under reduced pressure, combine the filtrate with the above-mentioned Hedyotis diffusa extract, and concentrate under reduced pressure at 45°C to dryness to obtain Hedyotis diffusa extract; S2.3: Dry and grind the root of Panax notoginseng into powder, add it into 60% ethanol solution at a solid-liquid ratio of 1g:45mL, heat and stir under reflux at 60℃ for 45min, repeat the extraction twice, combine the filtrates, concentrate under reduced pressure at 55℃ to dryness, and obtain the Panax notoginseng root extract; S3: Preparation of spent brewer's yeast extract S3.1: Add the waste brewer's yeast into 80% ethanol solution at a solid-liquid ratio of 1g:25mL, heat and reflux at 55℃ for 1.5h to obtain an extract; S3.2: The extract is transferred to a centrifuge, centrifuged at 4500 r / min for 15 min, the supernatant is recovered, and the supernatant is concentrated under reduced pressure at 35° C. to dryness to obtain a spent beer yeast extract; S4: Mixing to prepare anti-wrinkle whitening moisturizing lotion S4.1: 2 parts by weight of Rosa damascena flower oil and 13 parts by weight of soybean oil were stirred and mixed to obtain a mixed oil phase; S4.2: Add 2 parts by weight of cono peptide to 110 parts by weight of deionized water, stir thoroughly to dissolve, then add 9 parts by weight of litchi peel extract obtained in step S1.3, 4 parts by weight of Hedyotis diffusa extract obtained in step S2.2, 4 parts by weight of Panax notoginseng root extract obtained in step S2.3 and 11 parts by weight of spent brewer's yeast extract obtained in step S3.2, stir thoroughly to mix, and obtain a mixed aqueous phase; S4.3: Add the above mixed oil phase to the above mixed water phase, mix at a rate of 11000 r / min for 4 min using a high-speed disperser, and then treat twice at a pressure of 95 MPa using a high-pressure nano homogenizer to obtain an anti-wrinkle, whitening and moisturizing lotion.

[0024] Example 3 A preparation process of an anti-wrinkle, whitening and moisturizing lotion containing cono peptide, such as Figure 1 As shown, the following steps are included: S1: Preparation of litchi peel extract S1.1: After washing and drying the litchi peel, add it into a grinder and grind it thoroughly to obtain litchi peel powder; S1.2: Add the above litchi peel powder to 80% ethanol solution at a material-liquid ratio of 1g:40mL, stir and mix evenly, heat and reflux at 70°C and 200W ultrasonic power for 2h, and filter to obtain a primary litchi peel extract and a primary litchi peel residue; S1.3: Add the above litchi peel residue to 80% ethanol solution at a solid-liquid ratio of 1g:30mL, heat and reflux at 70°C and 150W ultrasonic power for 2h, filter, combine the filtrate with the above litchi peel extract, and concentrate under reduced pressure at 50°C to dryness to obtain litchi peel extract; S2: Preparation of Hedyotis diffusa extract and Panax notoginseng root extract S2.1: After washing, drying and grinding Hedyotis diffusa into powder, the powder is added into 75% ethanol solution at a solid-liquid ratio of 1:40 mL, heated and stirred at 80° C. for 2 h under reflux, and filtered under reduced pressure to obtain a primary Hedyotis diffusa extract and a primary Hedyotis diffusa residue; S2.2: Add the above-mentioned Hedyotis diffusa filter residue to 75% ethanol solution at a solid-liquid ratio of 1g:40mL, heat and stir under reflux at 80°C for 2h, filter under reduced pressure, combine the filtrate with the above-mentioned Hedyotis diffusa extract, and concentrate under reduced pressure at 50°C to dryness to obtain Hedyotis diffusa extract; S2.3: Dry and grind the root of Panax notoginseng into powder, add it into 60% ethanol solution at a solid-liquid ratio of 1g:50mL, heat and stir under reflux extraction at 65℃ for 50min, repeat the extraction twice, combine the filtrates, concentrate under reduced pressure at 60℃ to dryness, and obtain the Panax notoginseng root extract; S3: Preparation of spent brewer's yeast extract S3.1: Add the waste brewer's yeast into 80% ethanol solution at a solid-liquid ratio of 1g:30mL, heat and reflux at 60℃ for 2h to obtain an extract; S3.2: The extract is transferred to a centrifuge, centrifuged at 5000 r / min for 20 min, the supernatant is recovered, and the supernatant is concentrated under reduced pressure at 40° C. to dryness to obtain a spent beer yeast extract; S4: Mixing to prepare anti-wrinkle whitening moisturizing lotion S4.1: 2-3 parts by weight of Rosa damascena flower oil and 14 parts by weight of soybean oil are stirred and mixed to obtain a mixed oil phase; S4.2: Add 2-3 parts by weight of cono peptide to 120 parts by weight of deionized water, stir thoroughly to dissolve, then add 10 parts by weight of litchi peel extract obtained in step S1.3, 5 parts by weight of Hedyotis diffusa extract obtained in step S2.2, 5 parts by weight of Panax notoginseng root extract obtained in step S2.3 and 12 parts by weight of spent brewer's yeast extract obtained in step S3.2, stir thoroughly to mix, and obtain a mixed aqueous phase; S4.3: Add the above mixed oil phase to the above mixed water phase, mix at a rate of 12000 r / min for 5 min using a high-speed disperser, and then treat twice at a pressure of 100 MPa using a high-pressure nano homogenizer to obtain an anti-wrinkle, whitening and moisturizing lotion.

[0025] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that the notoginseng root extract in step S4.2 is replaced by an equal amount of conopeptide.

[0026] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that the cono peptide in step S4.2 is replaced by an equal amount of Panax notoginseng root extract.

[0027] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is that the litchi peel extract in step S4.2 is replaced by an equal amount of Hedyotis diffusa extract.

[0028] Comparative Example 4 The difference between Comparative Example 4 and Example 1 is that the Hedyotis diffusa extract in step S4.2 is replaced by an equal amount of litchi fruit extract.

[0029] Comparative Example 5 The difference between Comparative Example 5 and Example 1 is that the Hedyotis diffusa extract in step S4.2 is replaced by an equal amount of waste brewer's yeast extract.

[0030] Comparative Example 6 The difference between Comparative Example 6 and Example 1 is that the waste brewer's yeast extract in step S4.2 is replaced by an equal amount of Hedyotis diffusa extract.

[0031] Comparative Example 7 The difference between this comparative example 7 and example 1 is that the damask rosa flower oil in step S4.1 is removed, and conopeptide of the same mass as the damask rosa flower oil is added in step S4.2.

[0032] Comparative Example 8 The difference between Comparative Example 8 and Example 1 is that the cono peptide in step S4.2 is removed, and the damask rosa flower oil with the same mass as the cono peptide is added in step S4.1.

[0033] Test example Test 1: Wild AB zebrafish embryos at 2 dpf (days post fertilization) were tested. A blank control group, a model control group, and a test group (the test group included Example 1-3 groups and Comparative Example 1-2 groups) were set up in the test, and placed in a 6-well cell culture plate, with 1 embryo per well. The test solution was prepared according to Table 1, and 3 mL was added to each well. After the administration was completed, the 6-well plate was placed in a 28°C incubator in the dark for 2 hours.

[0034] Table 1: Zebrafish experimental groups and experimental conditions

[0035] The experimental group was exposed to ultraviolet light three times, each exposure time was 15 minutes, with an interval of 30 minutes, and the radiation dose was (2.07±0.18) J / cm 2 The total radiation dose is (6.21±0.54) J / cm 2, and then incubate in a dark incubator for 22 hours. After 22 hours, remove the 6-well plate, use tricaine for anesthesia, and then use a glass slide loaded with 2% methylcellulose to swing the fish. Then use a stereo microscope to take photos of the tail fin behind the cloaca of the young fish, and use the software Image J to measure the tail fin area of ​​each fish. The formula for calculating the inhibition rate of zebrafish tail fin shrinkage is as follows: Wrinkle inhibition rate = [(average caudal fin area of ​​zebrafish in the experimental group - average caudal fin area of ​​zebrafish in the model control group) / (average caudal fin area of ​​zebrafish in the blank control group - average caudal fin area of ​​zebrafish in the model control group)] × 100%. The results are shown in Table 2.

[0036] Table 2: Comparison of zebrafish tail fin wrinkle inhibition test results

[0037] Since human skin and zebrafish skin have similar structures, when zebrafish are irradiated with excessive ultraviolet rays, the skin of the zebrafish tail fin will shrink. As shown in Table 2, when a single conopeptide or a single Panax notoginseng root extract is added to Comparative Example 1 and Comparative Example 2, the inhibition rates of the reduction of the zebrafish tail fin area are approximately 22.46% and 18.26%, respectively, which are both lower than those in Example 1, indicating that the combination of conopeptide and Panax notoginseng root extract can achieve a synergistic anti-wrinkle effect.

[0038] Test 2: A 8cm×8cm glass plate was selected and dried in an oven to constant weight, and a layer of medical breathable tape was attached to it and then accurately weighed, and its mass was recorded as m1. The anti-wrinkle whitening and moisturizing emulsions prepared in Examples 1-3, Comparative Examples 3-4, and Comparative Examples 7-8 were respectively formulated into aqueous solutions with a mass fraction of 5%, and 100 μL was taken out with a pipette and evenly coated on the breathable tape and accurately weighed. The blank group was 100 μL of water, and its mass was recorded as m2. Then it was placed in a constant temperature box with a relative humidity of 42% and dried for 12 hours, then taken out and weighed, recorded as m3. The water loss rate of the sample was calculated: Water loss rate = (m2-m3) / (m2-m1)×100%. The results are shown in Table 3.

[0039] Table 3: Comparison of water loss test results

[0040] As can be seen from Table 3, when a single Hedyotis diffusa extract or a single litchi peel extract is added to Comparative Examples 3 and 4, the water loss rates of the obtained anti-wrinkle whitening moisturizing lotions within 12 hours are approximately 91.68% and 93.26%, respectively, which are much lower than that of Example 1. It can be seen that the Hedyotis diffusa extract and the litchi peel extract have a synergistic moisturizing effect. When the litchi peel extract concentrate and the Hedyotis diffusa extract concentrate are used simultaneously to prepare the lotion, the long-lasting moisturizing effect of the lotion can be further enhanced. In Comparative Example 7, after the taurine rosa flower oil was replaced with an equal amount of soybean oil and cono peptides of the same mass as the taurine rosa flower oil were added to the mixed aqueous phase, the water loss rate of the obtained anti-wrinkle whitening moisturizing emulsion within 12 hours was about 86.83%, which was lower than that in Example 1. In Comparative Example 8, after the cono peptides in the mixed aqueous phase were removed and the soybean oil of the same mass as the cono peptides was replaced with taurine rosa flower oil in the mixed oil phase, the water loss rate of the obtained anti-wrinkle whitening moisturizing emulsion within 12 hours was about 85.62%, which was also lower than that in Example 1. It can be seen that the taurine rosa flower oil and cono peptides can interact with each other to further improve the moisturizing effect of the emulsion.

[0041] Test 3: Take 1 mL of the anti-wrinkle whitening moisturizing lotion prepared in Examples 1-3 and Comparative Examples 5-8 in a test tube, add 1 mL of phosphate buffer (pH 6.8) and 1 mL of 0.03% tyrosine solution to each test tube, place in a 37°C water bath for 10 min, add 1 mL of tyrosinase solution, mix well and let stand for 25 min, measure the absorbance at a wavelength of 475 nm with a spectrophotometer (A), replace the tyrosinase with an equal volume of phosphate buffer to measure the absorbance at a wavelength of 475 nm (B); replace the anti-wrinkle whitening moisturizing lotion prepared in Examples 1-3 and Comparative Examples 5-8 with an equal volume of distilled water to measure the absorbance at a wavelength of 475 nm (C), and replace the tyrosinase solution with an equal volume of phosphate buffer to measure the absorbance at a wavelength of 475 nm (D). Calculate the inhibition rate of the sample on tyrosinase, and the calculation formula is as follows: Inhibition rate (%) = [(CD) - (AB)] / (CD) × 100%. The results are shown in Table 4.

[0042] Table 4: Comparison of tyrosinase inhibition test results

[0043] As can be seen from Table 4, the inhibition rates of the anti-wrinkle whitening moisturizing lotions prepared by using a single waste beer yeast extract and a single Hedyotis diffusa extract on tyrosinase in Comparative Examples 5 and 6 are approximately 68.73% and 72.37%, respectively, which are lower than that in Example 1. It can be seen that the combination of Hedyotis diffusa extract and waste beer yeast extract is beneficial to improving the overall whitening effect; In Comparative Example 7, after the taurine rosa flower oil was replaced with an equal amount of soybean oil and cono peptides of the same mass as the taurine rosa flower oil were added to the mixed aqueous phase, the inhibition rate of the anti-wrinkle whitening moisturizing lotion on tyrosinase was about 79.66%, which was lower than that in Example 1. In Comparative Example 8, after the cono peptides in the mixed aqueous phase were removed and the soybean oil of the same mass as the cono peptides was replaced with taurine rosa flower oil in the mixed oil phase, the inhibition rate of the anti-wrinkle whitening moisturizing lotion on tyrosinase was about 77.32%, which was also lower than that in Example 1. It can be seen that the taurine rosa flower oil and cono peptides can interact with each other, thereby further enhancing the whitening effect of the lotion.

[0044] In addition, according to the skin reaction grading standard of the skin occlusive patch test in the "Technical Specification for Safety of Cosmetics" (2015 edition), the anti-wrinkle and whitening moisturizing emulsions prepared in Examples 1-3 were subjected to human skin patch tests. The results showed that all 30 test subjects had negative skin reactions (scoring level 0), indicating that the anti-wrinkle and whitening moisturizing emulsions prepared in the present invention are safe and non-irritating to the human body.

[0045] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A process for preparing an anti-wrinkle, whitening and moisturizing emulsion containing cono peptide, characterized in that: The steps include: S1: Preparation of litchi peel extract The litchi peel is washed, dried and ground, and then stirred and mixed with an ethanol solution, and subjected to ultrasonic-assisted heating reflux extraction. After repeated extraction, the extracts are combined and concentrated under reduced pressure to obtain a litchi peel extract; S2: Preparation of Hedyotis diffusa extract and Panax notoginseng root extract Grinding Hedyotis diffusa and Panax notoginseng root into powder respectively and adding them into ethanol solution for heating and reflux extraction, and concentrating under reduced pressure to obtain Hedyotis diffusa extract and Panax notoginseng root extract; S3: Preparation of spent brewer's yeast extract Adding waste beer yeast into an ethanol solution, performing heating reflux extraction, recovering the supernatant after centrifugation, and concentrating under reduced pressure to obtain a waste beer yeast extract; S4: Mixing to prepare anti-wrinkle whitening moisturizing lotion First, rosa damascena flower oil and soybean oil are mixed to prepare a mixed oil phase, then cono peptide is dissolved in deionized water, and the above-mentioned litchi peel extract, oldenlandia diffusa extract, Panax notoginseng root extract and brewer's waste yeast extract are added to prepare a mixed water phase, and then the mixed oil phase is mixed with the mixed water phase to obtain an anti-wrinkle, whitening and moisturizing lotion.

2. The preparation process of the anti-wrinkle, whitening and moisturizing emulsion containing cono peptide according to claim 1, characterized in that: S1 specifically includes the following steps: S1.1: After washing and drying the litchi peel, add it into a grinder and grind it thoroughly to obtain litchi peel powder; S1.2: Add the above litchi peel powder to 80% ethanol solution at a material-liquid ratio of 1 g: (30-40) mL, stir and mix evenly, heat and reflux at 60-70°C and 100-200 W ultrasonic power for 1-2 h, and filter to obtain a primary litchi peel extract and a primary litchi peel residue; S1.3: Add the above litchi peel filter residue to 80% ethanol solution at a solid-liquid ratio of 1g: (20-30)mL, heat and reflux at 60-70℃ and 100-150W ultrasonic power for extraction for 1-2h. After filtration, combine the filtrate with the above litchi peel extract, and concentrate under reduced pressure at 40-50℃ to dryness to obtain litchi peel extract.

3. The preparation process of the anti-wrinkle, whitening and moisturizing emulsion containing cono peptide according to claim 2, characterized in that: S2 specifically includes the following steps: S2.1: After washing, drying and grinding Hedyotis diffusa into powder, add it into 75% ethanol solution at a solid-liquid ratio of 1:(30-40) mL, heat and stir under reflux extraction at 70-80°C for 1-2h, and filter under reduced pressure to obtain a primary Hedyotis diffusa extract and a primary Hedyotis diffusa residue; S2.2: Add the above-mentioned first Hedyotis diffusa filter residue to 75% ethanol solution at a solid-liquid ratio of 1 g: (30-40) mL, heat and stir under reflux at 70-80°C for 1-2 h, filter under reduced pressure, combine the filtrate with the above-mentioned first Hedyotis diffusa extract, and concentrate under reduced pressure at 40-50°C to dryness to obtain a Hedyotis diffusa extract; S2.3: Dry and grind the root of Panax notoginseng into powder, and add it into 60% ethanol solution at a solid-liquid ratio of 1g:(40-50)mL. Heat and stir under reflux extraction at 55-65℃ for 40-50min, repeat the extraction twice, combine the filtrate, and concentrate under reduced pressure at 50-60℃ to dryness to obtain the Panax notoginseng root extract.

4. The preparation process of the anti-wrinkle, whitening and moisturizing emulsion containing cono peptide according to claim 3, characterized in that: S3 specifically includes the following steps: S3.1: Add the waste brewer's yeast into 80% ethanol solution at a solid-liquid ratio of 1g: (20-30)mL, heat and reflux at 50-60℃ for 1-2h to obtain an extract; S3.2: The above extract is transferred to a centrifuge, centrifuged at a rate of 4000-5000 r / min for 10-20 min, the supernatant is recovered, and the supernatant is concentrated under reduced pressure at 30-40° C. to dryness to obtain a waste beer yeast extract.

5. The preparation process of the anti-wrinkle, whitening and moisturizing emulsion containing cono peptide according to claim 4, characterized in that: S4 specifically includes the following steps: S4.1: stirring and mixing the Rosa damascena flower oil and soybean oil to obtain a mixed oil phase; S4.2: Add cono peptide to deionized water, stir thoroughly to dissolve, then add litchi peel extract obtained in step S1.3, Hedyotis diffusa extract obtained in step S2.2, notoginseng root extract obtained in step S2.3 and brewer's waste yeast extract obtained in step S3.2, stir thoroughly to mix, and obtain a mixed aqueous phase; S4.3: Add the above mixed oil phase into the above mixed water phase, mix for 3-5 minutes using a high-speed disperser, and then process using a high-pressure nano homogenizer to obtain an anti-wrinkle, whitening and moisturizing lotion.

6. The preparation process of the anti-wrinkle, whitening and moisturizing emulsion containing cono peptide according to claim 5, characterized in that: The anti-wrinkle, whitening and moisturizing lotion comprises, by weight: 2-3 parts of cono peptide, 8-10 parts of litchi peel extract, 3-5 parts of oldenlandia diffusa extract, 3-5 parts of notoginseng root extract, 10-12 parts of brewer's waste yeast extract, 2-3 parts of damascena rose flower oil, 12-14 parts of soybean oil and 100-120 parts of deionized water.

7. The preparation process of the anti-wrinkle, whitening and moisturizing emulsion containing cono peptide according to claim 5, characterized in that: The speed of the high-speed disperser is 10000-12000r / min.

8. The preparation process of the anti-wrinkle, whitening and moisturizing emulsion containing cono peptide according to claim 5, characterized in that: The processing pressure of the high-pressure nano homogenizer is 90-100 MPa and the number of processing times is 2 times.

9. An anti-wrinkle, whitening and moisturizing lotion containing cono peptide, characterized in that: The anti-wrinkle, whitening and moisturizing emulsion is prepared by the preparation process of the cono peptide-containing emulsion according to any one of claims 1 to 8.

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