High-oxidation-resistance Tibetan essential oil / cream based on ancient-method soaked Tibetan wormwood and application of high-oxidation-resistance Tibetan essential oil / cream

Abstract

The invention belongs to the technical field of essential oil, and particularly relates to high-oxidation-resistance Tibetan essential oil / cream based on ancient method soaked wormwood and application, and the high-oxidation-resistance Tibetan essential oil / cream comprises the following components in parts by mass: 4-6 parts of ghee, 9-12 parts of wormwood and 50-100 parts of vegetable oil. Preferably, the traditional Chinese medicine composition also comprises 0.4-0.5 part of sandalwood. The prepared essential oil is strong in skin oxidation resistance, has the effects of relieving swelling and pain, stopping bleeding, promoting qi to activate blood, warming channels and expelling cold, can inhibit bacteria, resist inflammation, relieve pain and the like, and is wide in application.

Description

Technical Field

[0001] The invention belongs to the technical field of essential oils, and in particular relates to a high-antioxidant Tibetan essential oil / ointment based on an ancient method of soaking Tibetan wormwood and an application thereof. Background Art

[0002] According to Tibetan medicine, the skin is an important organ of the human body and is closely related to the three factors in the body. Antioxidation of the skin is an important aspect of maintaining skin health and delaying aging. The oxidation process of the skin will lead to the production of free radicals, which will damage the biological molecules in the skin cells, such as lipids, proteins and DNA, and thus accelerate skin aging, wrinkles, sagging and dullness. There are many kinds of antioxidant products on the market, but there are problems such as side effects that chemically synthesized antioxidants may bring, which are harmful to the human body.

[0003] Tibetan medicine believes that there are three major factors in the human body: Long, Triba and Bacon, as well as seven material bases. The three major factors dominate the movement and changes of the seven material bases and three excremental substances. Long, Triba and Bacon each have their own functions, and they coordinate with each other under certain conditions to maintain the normal physiological functions of the human body and carry out activities in a unified manner. When Long, Triba and Bacon are disordered, it is easy to cause diseases, namely the corresponding Long disease, Triba disease and Bacon disease. Among them, "Long" is similar to Qi, responsible for breathing, limb movement, blood circulation and other functions; "Triba" has the nature of fire, responsible for generating heat energy and digesting food; "Bacon" is related to the nature of water and soil, responsible for regulating the body's water and mucus. When the balance between "Long", "Triba" and "Bacon" is broken, it may cause meridian blockage and free radical damage to the skin. For example, an imbalance of "Long" may lead to qi stagnation and blood stasis; an imbalance of "Chi Ba" may cause heat syndrome or inflammation; an imbalance of "Bacon" may affect the body's water metabolism and digestive function, causing waste accumulation in the body, and then producing free radicals or affecting the skin's own antioxidant defense mechanism.

[0004] Therefore, premature skin aging, pigmentation and other oxidative phenomena, and blocked meridians can be seen as the result of the imbalance of the three major factors of "Long", "Chiba" and "Bacon". In treatment, it is necessary to adjust these three factors to restore the coordination and balance between them, so as to achieve the purpose of normal blood circulation, normal metabolism of skin cells, and unblocking meridians. Specific treatment methods may include acupuncture, massage or Chinese medicine conditioning.

[0005] Essential oils are generally used for application before external application of medications, application on the skin before balancing cupping or dragon cupping, application before massage, and application on mild skin burns, scalds, and insect bites. They have the effects of relaxing muscles and tendons, promoting qi and blood circulation, inhibiting bacteria and inflammation, reducing swelling and relieving pain. At the same time, they can also be used for daily health care, anti-oxidation, anti-radiation, and anti-aging. However, most of the essential oils currently used are chemical drugs, and their side effects are relatively large.

[0006] The specification of Chinese invention No. 201910608276.0 discloses a composite wormwood essential oil with anti-oxidation and anti-aging effects. The A component mixed essential oil is made of the following raw materials by volume: 40 to 45 parts of wormwood essential oil, 12 to 20 parts of sweet almond oil, 10 to 18 parts of aloe oil, 6 to 15 parts of perilla oil, 1 to 5 parts of ganoderma lucidum essential oil, 1 to 5 parts of rhodiola rosea essential oil, 1 to 5 parts of safflower seed oil, and 1 to 5 parts of ginkgo leaf essential oil; the B component mixed essential oil is made of the following raw materials by volume: 12 to 20 parts of wheat germ oil, 10 to 18 parts of jojoba oil, 6 to 15 parts of grape seed oil, 6 to 15 parts of rosehip oil, 1 to 5 parts of geranium essential oil, and 1 to 5 parts of jasmine essential oil. No artificial chemicals are added, it is a pure natural herbal essential oil, safe and non-allergic. It has the effects of promoting skin cell regeneration, restoring skin elasticity and delaying aging.

[0007] However, the patented technology has many complex raw material types, and it is impossible to determine the compatibility of each component. The function is unclear and the effect is not very obvious. In addition, some materials are not widely used because of the limited user groups. In addition, the quality of the raw material source needs to be considered.

[0008] The Tibetan area has formed rich medicinal resources due to its special plateau geographical and climatic conditions. From the tropical rainforest in southeastern Tibet to the vast grassland in northern Tibet, it reflects the biological resources of tropical, temperate and alpine zones respectively. The main cold and lack of oxygen, strong light and large temperature difference between day and night enable the ecological extraction of drugs in the natural environment. Summary of the invention

[0009] In order to solve the above technical problems, the present invention provides a high antioxidant Tibetan essential oil / ointment and application based on the ancient method of soaking Tibetan wormwood. The prepared essential oil material has a simple composition, and the main raw materials come from the Tibetan area. It has obvious anti-oxidation effect on the skin, and can also reduce swelling and relieve pain, stop bleeding, promote qi and blood circulation, warm the meridians and dispel cold. It can be antibacterial, anti-inflammatory and analgesic, etc., and has a wide range of uses; the preparation method is simple, no chemical agents are used, it is safe to use, and it has a wide range of applicability.

[0010] The present invention solves the above technical problems and provides a high antioxidant Tibetan essential oil / ointment based on the ancient method of soaking Tibetan wormwood, comprising the following components in parts by weight: 4-6 parts of ghee, 9-12 parts of Tibetan wormwood and 50-100 parts of vegetable oil.

[0011] In the optimized solution, the ghee comprises 5 parts, the Tibetan wormwood comprises 10 parts and the vegetable oil comprises 50-100 parts.

[0012] The Tibetan wormwood is Tibetan wormwood from Medog, Tibet. The sunshine time in Tibetan areas is long, and the picking time is from April to September.

[0013] The Tibetan mugwort grows in high altitude, cold and oxygen-deficient areas. It contains high essential oil and fiber content, and has unique medicinal value and aroma. It also has rich and high biological activity, which is 2-5 times higher than that of Tibetan mugwort in the plains.

[0014] The ghee is old ghee and is used for external application.

[0015] The ghee is 5-year-old Tibetan medicine ghee.

[0016] The Tibetan essential oil / cream also includes 0.4-0.5 parts of sandalwood extract.

[0017] The Tibetan essential oil / cream also includes 0.5 parts of Artemisia annua.

[0018] One part of amber is added to the Tibetan essential oil / cream to prepare a thick paste.

[0019] Tibetan wormwood and ghee are unique plants and food resources in Tibet, and together with sandalwood spices, they have a unique position in traditional medicine and culture. The combination of the three has a significant antioxidant effect on the skin.

[0020] The Tibetan wormwood is mature, high-quality, and fresh Tibetan wormwood, which is rich in antioxidants, such as vitamin E, rutin, flavonoids, etc. Flavonoid compounds have antioxidant activity, can remove and neutralize free radicals, and reduce the damage of oxidative stress to skin cells. It has strong antioxidant capacity, can prevent skin aging, promote skin elasticity, and delay skin aging; and the whole plant is used as medicine, which has the effects of warming the meridians, removing dampness, dispelling cold, stopping bleeding, anti-inflammatory and anti-allergic, and can pass the twelve meridians, run the three yin meridians, regulate qi and blood, and expel cold and dampness; it promotes the circulation of qi and blood through the characteristics of warming the meridians, thereby improving the pain caused by cold stagnation in the meridians.

[0021] Tibetan mugwort mainly grows in the Tibetan areas of southwest China, where the environment is characterized by high altitude, strong ultraviolet rays, and large temperature differences between day and night. The low oxygen and low temperature environment in high altitude areas may affect the growth and development of Tibetan mugwort, causing it to differ from ordinary mugwort in terms of physiological characteristics and chemical composition. Strong ultraviolet rays may prompt Tibetan mugwort to produce more protective substances, such as certain flavonoid compounds, which may play a certain role in its adaptation to the environment and medicinal effects. The large temperature difference between day and night is conducive to the accumulation of substances in the plant body, making the content of effective ingredients in Tibetan mugwort different from that in ordinary mugwort. In addition, soil factors such as soil type and mineral content in Tibetan areas will also affect the growth of Tibetan mugwort, resulting in differences in root development, nutrient absorption, etc. from ordinary mugwort.

[0022] Ghee is the essence of Tibetan food, which is fat extracted from cow's milk and goat's milk. It is cool in nature, can strengthen muscles, moisturize skin, and remove tribulations; old ghee can improve intelligence and brain function, warm the middle and replenish deficiency, and stimulate appetite and strengthen strength.

[0023] Ghee is rich in unsaturated fatty acids and other nutrients. Unsaturated fatty acids can enhance the antioxidant defense system of skin cells by regulating the lipid metabolism of skin cells. They can also participate in the composition of cell membranes, improve the fluidity of cell membranes, and play an active role in resisting free radical attacks. At the same time, some trace elements and vitamins in ghee may also contribute to its antioxidant properties. After a long period of storage, the 5-year-old ghee undergoes a natural aging process, and some components of the fat change, resulting in a more complex product.

[0024] Sandalwood is the heartwood of the Santalaceae family, an evergreen small tree, 6 to 9 meters tall. Sandalwood leaves, roots, and trunk fragments can be used to extract essential oils. It can regulate qi and regulate the middle, dispel cold and relieve pain, clear blood and resist inflammation; it can also fade scars and fine lines, moisturize the skin, and prevent wrinkles. Sandalwood is a balanced essential oil with 80-90% santalol. Santalol has a calming quality, which can soothe nervous tension and anxiety and make people relax.

[0025] The sandalwood leaf extract of the present invention has antioxidant and antibacterial activities, and its antioxidant activity is also strong, and it can remove DPPH free radicals. The extract contains polysaccharides, tannins, flavonoids, phenols, organic acids, amino acids, polypeptides, proteins and other components. These components may work synergistically to exert antioxidant effects and have a certain effect on skin antioxidant.

[0026] Tibetan Artemisia annua, an annual herb; Artemisia annua decoction has inhibitory effects on many kinds of bacteria.

[0027] Amber, also known as blood amber, blood amber, red amber, light amber. Nature and flavor: sweet, flat; calms the nerves, promotes blood circulation and removes blood stasis, promotes diuresis and relieves stranguria, and also acts as an emulsifier.

[0028] The vegetable oil is a small molecule vegetable oil.

[0029] The small molecule vegetable oil is camellia oil, tea seed oil, sesame oil, olive oil or sweet almond oil.

[0030] A method for preparing a Tibetan essential oil / cream in the present invention comprises the following steps: (1) Material selection and processing: Select fresh Tibetan wormwood from April to August, transparent glass sealed jars, high-quality vegetable oil, ghee, and disinfectant alcohol; Material processing: Wash and dry the fresh Tibetan wormwood for later use, and disinfect the transparent glass sealing cover with disinfectant alcohol for later use; (2) Crushing: crush the processed Tibetan wormwood with a grinder to make it into powder, and sieve it with a sieve for later use; (3) Soaking: Put the Tibetan wormwood powder into a reaction tank, then add an appropriate amount of vegetable oil and ghee to soak the Tibetan wormwood powder; Sealing: Seal the opening of the reaction tank with plastic wrap and cover it with a sealing lid; ghee is in solid state and is mixed with vegetable oil after melting.

[0031] (4) Mixing: Stirring the materials in the reaction tank to fully mix the Tibetan wormwood, vegetable oil and ghee; Reaction: Place the reaction tank indoors, stir once every two weeks to obtain Tibetan wormwood oil, and let it stand for more than one month before use; Filtration: Filter the completely reacted Tibetan wormwood oil with an oil filter cloth to filter out excess impurities to obtain pure Tibetan wormwood oil; Packaging: Put the pure Tibetan wormwood oil into a sterilized transparent glass sealed jar.

[0032] The grinding speed of the grinder is 3900 r / min.

[0033] The sieve is a 300 mesh sieve.

[0034] The oil filter cloth is made of gauze or non-woven fabric.

[0035] Preparation method of sandalwood extract: extract sandalwood leaves with 80% ethanol, and obtain sandalwood extract after extraction, separation, drying and other steps, pH 6-8. It has strong antioxidant and antibacterial effects.

[0036] Tibetan Artemisia annua is added to the oil together with Tibetan mugwort in step (3) and soaked.

[0037] After the amber is melted, add it to the essential oil, heat it, and cool it into a thick paste.

[0038] The Tibetan essential oil / cream of the present invention is used in the preparation of skin antioxidant care products and the preparation of swelling-reducing, analgesic and pain-relieving products.

[0039] The substances constituting the antioxidant of the present invention are all daily edible substances, are green and pure natural items, have no side effects on the human body, and are safe to use.

[0040] The Tibetan essential oil / ointment of the present invention has obvious anti-oxidation effect on the skin, can also reduce swelling and relieve pain, stop bleeding, promote qi and blood circulation, warm the meridians and dispel cold, and can also inhibit bacteria, resist inflammation and relieve pain, and has a wide range of uses. DETAILED DESCRIPTION

[0041] The present invention is further described below in conjunction with specific embodiments: The vegetable oil is camellia oil, tea seed oil, sesame oil, olive oil or sweet almond oil; the oil filter cloth is gauze or non-woven fabric, and the capacity of the transparent glass sealed jar is 250ml or 500ml. Example

[0042] (1) Material selection: Select fresh Tibetan mugwort from April to August (hereinafter referred to as Tibetan mugwort), transparent glass sealed jars, high-quality vegetable oil, 5-year-old ghee, and disinfectant alcohol; Material processing: Wash and dry the fresh Tibetan mugwort for later use, and disinfect the transparent glass sealed cover with disinfectant alcohol for later use; (2) Crushing: crush the processed Tibetan wormwood with a grinder at a grinding speed of 3900 r / min to make it into powder, and sieve it with a 300-mesh sieve for later use; (3) Soaking: put 100 parts of Tibetan wormwood powder into a reaction tank, then add 500 parts of vegetable oil and 50 parts of ghee, and soak; Sealing: seal the opening of the reaction tank with plastic wrap and cover it with a sealing cover; (4) Mixing: Stirring the materials in the reaction tank to fully mix the Tibetan wormwood, vegetable oil and ghee; Reaction: Placing the reaction tank indoors, stirring once every 2 weeks to obtain the grass oil, and taking it out after standing for 1 month; (5) Filtration: Filter the completely reacted Tibetan wormwood oil with an oil filter cloth to remove excess impurities to obtain pure wormwood oil; Packaging: Pack the pure wormwood oil into a sterilized transparent glass sealed jar.

[0043] Tibetan mugwort is a traditional Chinese medicinal material originating from the Tibetan areas of southwest China. In the lives of Tibetans, mugwort is widely used in medicine, health care and daily life. It is praised as a "magic plant" with good antioxidant properties. It can help remove free radicals, slow down skin aging and repair the skin. Tibetan mugwort plays a role under the theoretical system of Tibetan medicine. Example

[0044] (1) Material selection: Select fresh Tibetan wormwood from April to August, transparent glass sealed jars, high-quality vegetable oil, ghee, and disinfectant alcohol; Material processing: Wash and dry the fresh Tibetan wormwood for later use, and disinfect the transparent glass sealed lid with disinfectant alcohol for later use; (2) Crushing: crush the processed Tibetan wormwood with a grinder at a grinding speed of 3900 r / min to make it into powder, and sieve it with a 300-mesh sieve for later use; (3) Soaking: put 100 parts of Tibetan wormwood powder into a reaction tank, then add 750 parts of vegetable oil and 50 parts of ghee, and soak; Sealing: seal the opening of the reaction tank with plastic wrap and cover it with a sealing cover; (4) Mixing: Stirring the materials in the reaction tank to fully mix the Tibetan wormwood, vegetable oil and ghee; Reaction: Placing the reaction tank indoors, stirring once every two weeks to obtain the grass oil, and taking it out after standing for two months; (5) Filtration: Filter the completely reacted herb oil with an oil filter cloth to remove excess impurities to obtain pure Tibetan wormwood oil; Packaging: Pack the pure herb oil into a sterilized transparent glass sealed jar.

[0045] Sandalwood essential oil is suitable for aging, dehydrated and dry skin. It can promote skin metabolism, speed up the renewal and growth of skin cells, accelerate the repair and healing of wounds and scars, and help repair damaged skin cells. At the same time, it also has good antibacterial effects, can improve skin inflammation and itching, and reduce inflammation and remove acne. Example

[0046] (1) Material selection: Select fresh Tibetan wormwood from April to August, transparent glass sealed jars, high-quality vegetable oil, ghee, and disinfectant alcohol; Material processing: Wash and dry the fresh Tibetan wormwood for later use, and disinfect the transparent glass sealed lid with disinfectant alcohol for later use; (2) Crushing: crush the processed Tibetan wormwood with a grinder at a grinding speed of 3900 r / min to make it into powder, and sieve it with a 300-mesh sieve for later use; (3) Soaking: taking 100 parts of Tibetan wormwood powder, putting the 100 parts of Tibetan wormwood powder into a reaction tank, and then adding 1000 parts of vegetable oil and 50 parts of ghee, and soaking; Sealing: sealing the opening of the reaction tank with plastic wrap and covering it with a sealing cover; (4) Mixing: Stirring the materials in the reaction tank to fully mix the Tibetan wormwood, vegetable oil and ghee; Reaction: Placing the reaction tank indoors, stirring once every 2 weeks to obtain the Tibetan wormwood oil, and taking it out after standing for 3 months; (5) Filtration: Filter the completely reacted herb oil with an oil filter cloth to remove excess impurities to obtain pure Tibetan wormwood oil; Packaging: Pack the pure herb oil into a sterilized transparent glass sealed jar. Example

[0047] (1) Material selection: Select fresh Tibetan wormwood from April to August, transparent glass sealed jars, high-quality vegetable oil, ghee, and disinfectant alcohol; Material processing: Wash and dry the fresh Tibetan wormwood for later use, and disinfect the transparent glass sealed lid with disinfectant alcohol for later use; (2) Crushing: crush the processed Tibetan wormwood with a grinder at a grinding speed of 3900 r / min to make it into powder, and sieve it with a 300-mesh sieve for later use; (3) Soaking: put 100 parts of Tibetan wormwood powder into a reaction tank, then add 500 parts of vegetable oil and 50 parts of ghee, and soak; Sealing: seal the opening of the reaction tank with plastic wrap and cover it with a sealing cover; (4) Mixing: Stirring the materials in the reaction tank to fully mix the Tibetan wormwood, vegetable oil and ghee; Reaction: Placing the reaction tank indoors, stirring once every 2 weeks to obtain the grass oil, and taking it out after standing for 1 month; (5) Filtration: Filter the completely reacted Tibetan wormwood with an oil filter cloth to remove excess impurities; (6) Add 5 parts of sandalwood extract to obtain a mixed grass oil composition; and then package: the grass essential oil composition is placed in a sterilized transparent glass sealed jar.

[0048] Preparation method of sandalwood extract: extract sandalwood leaves with 80% ethanol, and obtain sandalwood extract through extraction, separation, drying and other steps, with pH 6-8.

[0049] The combination of Tibetan wormwood, aged ghee and sandalwood essential oil is applied externally. Through the absorption and induction of the skin, it acts on the three factors of the human body. By balancing the three factors of Long, Triba and Bacon, it improves the internal environment of the body and the local state of the skin as a whole, making the internal environment of skin cells more stable, thereby improving the skin's antioxidant capacity. If Long is unbalanced, it may lead to a decrease in the skin's defense function and make the skin more vulnerable to free radical attacks. After Tibetan wormwood adjusts Long balance, the skin's defense function is improved, thereby improving the skin's antioxidant capacity. Similarly, the balanced regulation of Triba and Bacon also helps maintain the normal metabolism and function of skin cells and reduce the occurrence of oxidative stress. Example

[0050] (1) Material selection: Select fresh Tibetan mugwort from April to August, transparent glass sealed jars, high-quality vegetable oil, ghee, and disinfectant alcohol; Material processing: Wash and dry fresh Tibetan mugwort and Artemisia annua for later use, and disinfect the transparent glass sealed lid with disinfectant alcohol for later use; (2) Grinding: Grind the processed Tibetan wormwood and Artemisia annua into powder using a grinder at a grinding speed of 3900 r / min, and sieve with a 300-mesh sieve for later use; (3) Soaking: put 90 parts of Tibetan mugwort powder and 5 parts of Artemisia annua into a reaction tank, then add 600 parts of vegetable oil and 40 parts of ghee, and soak; Sealing: seal the opening of the reaction tank with plastic wrap and cover it with a sealing cover; (4) Mixing: Stirring the materials in the reaction tank to fully mix the Tibetan mugwort, Artemisia annua, vegetable oil and butter; Reaction: Placing the reaction tank indoors, stirring once every 2 weeks to obtain the grass oil, and taking it out after standing for 1 month; (5) Filtration: Filter the completely reacted Tibetan wormwood and artemisia annua oil with an oil filter cloth to remove excess impurities and obtain pure herb oil; Packaging: Pack the pure herb oil into a sterilized transparent glass sealed jar. Example

[0051] (1) Material selection: Select fresh Tibetan mugwort from April to August, transparent glass sealed jars, high-quality vegetable oil, ghee, and disinfectant alcohol; Material processing: Wash and dry fresh Tibetan mugwort and Artemisia annua for later use, and disinfect the transparent glass sealed lid with disinfectant alcohol for later use; (2) Grinding: Grind the processed Tibetan wormwood and Artemisia annua into powder using a grinder at a grinding speed of 3900 r / min, and sieve with a 300-mesh sieve for later use; (3) Soaking: put 120 parts of Tibetan mugwort powder and 5 parts of Artemisia annua into a reaction tank, then add 500 parts of vegetable oil and 60 parts of ghee, and soak; Sealing: seal the opening of the reaction tank with plastic wrap and cover it with a sealing cover; (4) Mixing: Stirring the materials in the reaction tank to fully mix the Tibetan mugwort, Artemisia annua, vegetable oil and butter; Reaction: Placing the reaction tank indoors, stirring once every 2 weeks to obtain the grass oil, and taking it out after standing for 1 month; (5) Filtration: Filter the completely reacted Tibetan wormwood and artemisia annua oil with an oil filter cloth to remove excess impurities and obtain pure herb oil; (6) Add 5 parts of sandalwood extract to obtain a mixed grass oil composition; and then package: the grass essential oil composition is placed in a sterilized transparent glass sealed jar. Example

[0052] After 10 parts of amber is heated and melted, it is added to the essential oils in Examples 1-6 above and stirred evenly, and cooled to a thick paste. Heat in a water bath for 10 minutes at a temperature of 30-80°C.

[0053] When used in the present invention, the herbal oil is directly applied to the skin, and massaged for 5 minutes to allow the skin to absorb. Usually, no special washing is required after application unless discomfort such as redness, swelling, itching, etc. occurs. If discomfort occurs, use soap to wash the applied area.

[0054] The tools used during the storage period should be disinfected in advance to extend the shelf life; transparent glass bottles can be sealed to observe whether the color and smell of the Tibetan wormwood oil change.

[0055] Experimental - In vitro test A DPPH free radical scavenging experiment was performed: the essential oil of Example 1 of the present invention was reacted with a DPPH solution, and the free radical scavenging rate was calculated by detecting the absorbance change of the solution at a specific wavelength after the reaction. The specific steps are as follows.

[0056] Prepare DPPH solution: weigh 0.002 g DPPH, dissolve it in 50 mL anhydrous ethanol, shake thoroughly to dissolve DPPH completely; measure the absorbance at 519 nm with a visible spectrophotometer, adjust the solution concentration so that the absorbance is around 0.9; store in a dark place; Preparation of essential oil solution: dissolve the essential oils in Examples 1-6 of the present invention in anhydrous ethanol to prepare a 1 mg / mL solution.

[0057] Determine absorbance: Take 100 μL of DPPH solution, add 100 μL of the essential oil solution of Examples 1-6 respectively, mix well; measure the absorbance at 519 nm with a visible spectrophotometer, and record the data.

[0058] Blank control: Take 100 μL of DPPH solution, add 100 μL of anhydrous ethanol, mix well; react at room temperature in a dark place for 30 minutes, measure the absorbance at 519 nm with a visible spectrophotometer, and record the data.

[0059] Positive control: Prepare 0.5 mg / mL vitamin C (Vc) solution as a positive control, take 100 μL DPPH solution, add 100 μL Vc solution, mix well; react at room temperature in the dark for 30 minutes, measure the absorbance at 519 nm with a visible spectrophotometer, and record the data.

[0060] Calculate the DPPH free radical scavenging rate: scavenging rate (%) = (1-(A sample-A blank) / A control) × 100%, where: A sample: absorbance after adding essential oil, A blank: absorbance after adding anhydrous ethanol, A control: absorbance of DPPH solution without adding any sample.

[0061] The measurement results are shown in Table 1 below: Table 1 Antioxidant test results of various examples

[0062] Compared with known antioxidants, the results show that the composition of the present invention has a higher free radical scavenging rate, proving that it has good antioxidant properties.

[0063] Test 2 The following test example is an actual effect example of using the essential oil in Example 1: Example 1: Two tourists, male, aged 40-42, suffered from facial burns and peeling, redness and swelling, which were cured after using the product; Example 2: A 37-year-old patient had grade 4 shoulder, neck and back pain. Before the balanced cupping, he applied Tibetan wormwood oil for massage, and used walking cupping and shaking cupping on the external application. The patient's pain disappeared when he returned for a visit on the third day. Example 3: A 3-year-old boy was bitten by a mosquito and applied Tibetan wormwood oil. The redness and swelling subsided the next day, 2 days earlier than the other oil controls. Example 4: 6 female patients, used it for UV protection, and the anti-radiation and anti-oxidation effects were significantly better than other cosmetics; Example 5: Clinical trials showed that the product was effective in external massage and before treatment, and had a significant analgesic effect.

[0064] Test 3 Experimental groups and control groups 1-4 were set, and the experimental group was the formulation and preparation method in Example 1; Control group 1 is the same as Example 1 in other contents, wherein the wormwood leaf is plain common wormwood leaf; Control group 2 is the same as Example 1 except that ghee is not present; Control group 3 is the same as Example 1 in other aspects, wherein the ghee is freshly made ghee; Control group 4 is the same as Example 1 in other contents, except that there is no ghee and the mugwort leaves are plain common mugwort leaves.

[0065] DPPH free radical scavenging test: The test steps are as in Test 1, and the samples are essential oils 1-4 in the experimental group and the control group. The test results are shown in Table 2 below: Table 2 Antioxidant test results of experimental group and control group

[0066] As can be seen from Table 2 above, the raw materials in the composition of the present invention cooperate with each other, so that the composition has a high free radical scavenging rate as a whole and has a good antioxidant effect.

[0067] Test 4 Statistical test of skin oxidation: Experimental subjects: 30 women around 35 years old with similar skin quality were selected.

[0068] Skin condition before the test: average luster, mostly even color, with some yellowing, dark yellowing, and blacking, dry, slightly rough, and loose.

[0069] The essential oil prepared in Example 1 of the present invention was applied to the skin area of ​​the left arm of each person once a day. After 20 days, the effect of external application on skin health conditions, such as skin moisture content, elasticity, color, etc., was observed. At the same time, the oxidative stress indicators malondialdehyde (MDA) content and superoxide dismutase (SOD) activity of the skin were detected, as shown in the following Table 3: Table 3

[0070] Among them, in Table 3 above, the skin moisture content test method is: put the test pen on the moisture measuring instrument on the skin, and write down the displayed moisture content value. The moisture measuring instrument is German CK.

[0071] Elasticity test: finger pressure and touch.

[0072] Color test: visual inspection.

[0073] MDA content test and SOD activity test: Take 20 SPE-grade healthy Kunming rats, 160-180g, half male and half female. 20 rats were used as the experimental group of the 12-month-old control group and the 12-month-old medium-dose group, with 10 rats in each group. The rats were fed and watered under the same natural conditions, and the experiment was started after adaptive feeding. Before the experiment, 3.5cm*3.5cm of hair on the central skin of the back of the mouse was removed with a shaver. The experimental group was coated with 1g of Tibetan wormwood essential oil in Example 1, and the control group was coated with deionized water, and protected with plastic wrap for 1h. Apply once a day, 1g each time, and the rats were killed after 28 consecutive days of application to measure the MDA and SOD values ​​at their experimental skin.

[0074] Preparation of skin homogenate and determination of MDA and SOD: Take about 0.6g of skin tissue block after hair removal from the back, rinse with precooled 1% sodium chloride solution, remove subcutaneous fat and other connective tissues, wipe with filter paper and weigh, cut the tissue block into pieces and pour it into a homogenization tube, add 10 times the mass of the tissue block in precooled 1% sodium chloride solution, and homogenize it with an internal cutting tissue homogenizer for 12 minutes at a temperature of 0-2°C to make a tissue homogenate, which is completely broken and the cell contents are completely free in the liquid phase. The MDA and SOD in the skin homogenate were determined according to conventional techniques in the art. The MDA in the control group was 9.01 ± 0.35 mmol / g and the SOD was 33 ± 0.57 U / ml.

[0075] As can be seen from Table 3 above, Example 1 has brought significant effects in terms of skin moisture content, elasticity and color, and the essential oil of the present invention has obvious skin care effects. The results also show that after using the essential oil of the present invention, the SOD activity of the skin is greatly improved and the MDA content is reduced, indicating that the composition can well improve the antioxidant capacity of the skin in vivo.

[0076] The above implementation / test examples are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the invention.

Claims

1. A high antioxidant Tibetan essential oil / cream based on the ancient method of soaking Tibetan wormwood, characterized by: The invention comprises the following components in parts by weight: 4-6 parts of ghee, 9-12 parts of Tibetan wormwood and 50-100 parts of vegetable oil.

2. A high antioxidant Tibetan essential oil / cream based on the ancient method of soaking Tibetan wormwood according to claim 1, characterized in that: 5 parts of ghee, 10 parts of Tibetan wormwood and 50-100 parts of vegetable oil.

3. The high antioxidant Tibetan essential oil / cream based on the ancient method of soaking Tibetan wormwood according to claim 1, characterized in that: The Tibetan essential oil / cream also includes 0.5 parts of Artemisia annua.

4. The high antioxidant Tibetan essential oil / cream based on the ancient method of soaking Tibetan wormwood according to claim 1, characterized in that: The Tibetan essential oil / cream also includes 0.4-0.5 parts of sandalwood extract.

5. The high antioxidant Tibetan essential oil / cream based on the ancient method of soaking Tibetan wormwood according to claim 1, characterized in that: The Tibetan mugwort is the Medog mugwort from Tibet.

6. A high antioxidant Tibetan essential oil / cream based on the ancient method of soaking Tibetan wormwood according to any one of claims 1-5, characterized in that: One part of amber is added to the Tibetan essential oil / cream to prepare a thick paste.

7. The high antioxidant Tibetan essential oil / cream based on the ancient method of soaking Tibetan wormwood according to claim 1, characterized in that: The vegetable oil is small molecule vegetable oil, and the ghee is aged ghee.

8. The high antioxidant Tibetan essential oil / cream based on the ancient method of soaking Tibetan wormwood according to claim 7, characterized in that: The ghee is 5-year-old ghee.

9. The high antioxidant Tibetan essential oil / cream based on the ancient method of soaking Tibetan wormwood according to claim 7, characterized in that: The small molecule vegetable oil is one of camellia oil, tea seed oil, sesame oil, olive oil or sweet almond oil.

10. The use of a high antioxidant Tibetan essential oil / cream based on the ancient method of soaking Tibetan wormwood according to claim 1, characterized in that: Application in the preparation of skin antioxidant care products and the preparation of swelling, analgesia and pain relief products.

Images