Endoscope ESD postoperative wound repair film

Abstract

The invention discloses an endoscopic ESD (Electro-Static Discharge) postoperative wound repair film. The endoscopic ESD postoperative wound repair film is prepared from a component A, a component B and degradable polypropylene, the component A is prepared from the following raw materials in percentage by weight: animal-derived collagen, blood coagulation protein, snail mucus extract, a protein folding agent, a culture medium and green tea extract; the component B is prepared from the following raw materials in percentage by weight: sodium hyaluronate gel, chitosan, a conch sheath extract and an oyster extract; a raw material composed of the component A and the component B is coated on a thin film made of degradable polypropylene, and the endoscopic ESD postoperative wound repair film is obtained. The traditional Chinese medicine has the effects of sealing, stopping bleeding and promoting repair on postoperative wounds.

Description

Technical Field

[0001] The invention relates to the technical field of medical materials, and in particular to a wound repairing membrane after endoscopic ESD surgery. Background Art

[0002] Endoscopic submucosal dissection (ESD) is a minimally invasive technique that completely removes the diseased mucosa from the submucosal layer under endoscopy. It is an elective diagnostic surgery or radical surgery. The main purpose of ESD is to diagnose and treat early gastrointestinal tumors, and it has the advantage of completely removing a certain area of ​​superficial lesions at one time. However, ESD is often accompanied by risks such as bleeding and perforation during and after surgery. The time for wound healing varies depending on individual differences, the difficulty of surgical operation, the patient's overall health status and other factors. Generally speaking, it takes weeks to months for the wound after rectal ESD to heal completely. In the first week to two weeks after ESD, a surface covering called a granular matrix will form on the wound, and a small amount of exudate or mild redness and swelling may also appear locally. After the second week, the wound begins to heal gradually, and a new epithelial layer is formed in the process. Usually, the wound heals initially 4 to 6 weeks after surgery, but some small ulcers or scars may still exist.

[0003] After ESD, due to the loss of mucosal barrier function, the wound is exposed to the open lumen environment, which is prone to infection, ulceration, and even necrosis. Therefore, other methods or materials are often needed to assist wound closure and hemostasis after ESD. At present, the commonly used mechanical hemostasis (hemostatic clips, ligation, etc.) in clinical practice has limited effect on postoperative wound closure and hemostasis, and is prone to postoperative bleeding and perforation.

[0004] The existing research materials have the problem that the degradation of the covering material does not match the ulcer healing, and the effect of promoting ulcer healing is limited. In addition, due to the strong peristalsis and complex physiological structure of the digestive tract, the retention time of simple medical adhesives in situ is short, and there are problems such as inconvenient delivery, cumbersome surgical operations, and long surgical time. Therefore, it is urgent to develop a postoperative wound repair membrane for ESD to meet the requirements of clinical ESD. Summary of the invention

[0005] In order to solve the above problems, the present invention provides an endoscopic ESD postoperative wound repair membrane, which has the effects of sealing, hemostasis and promoting repair of the postoperative wound.

[0006] The technical solution of the present invention is: a wound repair membrane after endoscopic ESD surgery, which is composed of component A, component B and degradable polypropylene;

[0007] The component A is composed of the following raw materials in percentage by weight: animal-derived collagen, coagulation protein, snail mucus extract, protein folding agent, culture medium and green tea extract;

[0008] The component B is composed of the following raw materials in percentage by weight: sodium hyaluronate gel, chitosan, conch sheath extract and oyster extract;

[0009] The raw materials composed of component A and component B are coated on a film made of degradable polypropylene to obtain a wound repair film after endoscopic ESD surgery.

[0010] Furthermore, the mass ratio of component A to component B is 1:2-5.

[0011] Furthermore, the mass of the degradable polypropylene film is 0.001-0.01 of the mass of the raw materials consisting of component A and component B.

[0012] Furthermore, in the component A, the mass percentage of animal-derived collagen is 25% to 40%, the mass percentage of coagulation protein is 18% to 28%, the mass percentage of snail mucus extract is 7% to 22%; the mass percentage of protein folding agent is 8% to 16%; and the mass percentage of green tea extract is 5% to 15%.

[0013] Furthermore, in the B component, the mass percentage of sodium hyaluronate gel is 40% to 65%, the mass percentage of chitosan is 20% to 45%, the mass percentage of conch sheath extract is 5% to 30%, and the mass percentage of oyster extract is 5% to 30%.

[0014] Furthermore, the protein folding agent is glutathione or cysteine.

[0015] Furthermore, the culture medium is MCDB culture medium.

[0016] The animal-derived collagen and coagulation protein in the component A of the present invention have the effect of promoting wound growth, and the green tea extract has the effect of sterilization and antiviral.

[0017] The sodium hyaluronate gel and chitosan of the present invention have the following effects on skin repair: promoting wound healing, accelerating skin metabolism, isolating and inhibiting bacteria, improving skin immunity, and calming and soothing.

[0018] The conch sheath extract of the present invention has the effects of relieving pain, astringing moisture and healing sores, promoting wound healing and stopping bleeding.

[0019] The oyster extract of the present invention has the effects of calming the nerves, suppressing yang and replenishing yin, softening and dispersing lumps, and the like.

[0020] The present invention also provides a method for preparing a wound repair film after endoscopic ESD surgery, comprising the following steps:

[0021] 1) mixing the animal-derived collagen, coagulation protein, snail mucus extract and protein folding agent of component A with deionized water to obtain a mixed solution A;

[0022] 2) adding culture medium and green tea extract to mixed solution A, mixing well to obtain culture solution;

[0023] 3) dissolving the sodium hyaluronate gel of component B into deionized water, adding chitosan, conch sheath extract and oyster extract and mixing evenly to obtain a mixed solution B;

[0024] 4) adding the mixed solution B to the culture solution of step 2), mixing evenly, and storing at 2° C. for 14-20 hours to obtain a gel;

[0025] 5) Forming the degradable polypropylene into a film, and coating the gel obtained in step 4) on the degradable polypropylene film.

[0026] The beneficial effects of the present invention are:

[0027] 1) The wound repair membrane after endoscopic ESD surgery of the present invention is based on natural colloid, has good biocompatibility, good hemostasis, adhesion and self-repairing properties, and has the effects of sealing, hemostasis and promoting repair of postoperative wounds.

[0028] 2) The wound repair membrane after endoscopic ESD surgery of the present invention is used for wound repair, isolating external bacteria, which is beneficial to promoting wound healing and effectively preventing wound infection. DETAILED DESCRIPTION

[0029] The technical solution of the present invention will be clearly and completely described below through specific implementation methods.

[0030] Example 1

[0031] The wound repair membrane after endoscopic ESD surgery of the present invention is composed of component A, component B and degradable polypropylene;

[0032] Component A is composed of the following raw materials in weight percentage: animal-derived collagen, coagulation protein, snail mucus extract, protein folding agent, culture medium and green tea extract; the mass percentage of animal-derived collagen in component A is 25%, the mass percentage of coagulation protein is 28%, the mass percentage of snail mucus extract is 22%; the mass percentage of protein folding agent is 10%; and the mass percentage of green tea extract is 15%.

[0033] Component B is composed of the following raw materials in weight percentage: sodium hyaluronate gel, chitosan, conch sheath extract and oyster extract. The mass percentage of sodium hyaluronate gel in component B is 65%, the mass percentage of chitosan is 20%, the mass percentage of conch sheath extract is 5%, and the mass percentage of oyster extract is 10%.

[0034] In this embodiment, the protein folding agent is glutathione or cysteine.

[0035] The culture medium used was MCDB medium.

[0036] In this embodiment, the mass ratio of component A to component B is 1:2.

[0037] In this embodiment, the mass of the degradable polypropylene film is 0.001 of the mass of the raw materials consisting of the A component and the B component.

[0038] A method for preparing a wound repair film after endoscopic ESD surgery of the present invention comprises the following steps:

[0039] 1) mixing the animal-derived collagen, coagulation protein, snail mucus extract and protein folding agent of component A with deionized water to obtain a mixed solution A;

[0040] 2) Add culture medium and green tea extract to mixed solution A and mix well.

[0041] Homogenize to obtain culture solution;

[0042] 3) dissolving the sodium hyaluronate gel of component B into deionized water, adding chitosan, conch sheath extract and oyster extract and mixing evenly to obtain a mixed solution B;

[0043] 4) Add mixed solution B to the culture solution in step 2) and mix well.

[0044] Mix well and store at 2°C for 14-20 hours to obtain a gel;

[0045] 5) Forming the degradable polypropylene into a film, and coating the gel obtained in step 4) on the degradable polypropylene film.

[0046] Example 2

[0047] The wound repair membrane after endoscopic ESD surgery of the present invention is composed of component A, component B and degradable polypropylene;

[0048] Component A is composed of the following raw materials in weight percentage: animal-derived collagen, coagulation protein, snail mucus extract, protein folding agent, culture medium and green tea extract; the mass percentage of animal-derived collagen in component A is 35%, the mass percentage of coagulation protein is 20%, the mass percentage of snail mucus extract is 20%; the mass percentage of protein folding agent is 15%; and the mass percentage of green tea extract is 10%.

[0049] Component B is composed of the following raw materials in weight percentage: sodium hyaluronate gel, chitosan, conch sheath extract and oyster extract. The mass percentage of sodium hyaluronate gel in component B is 40%, the mass percentage of chitosan is 35%, the mass percentage of conch sheath extract is 15%, and the mass percentage of oyster extract is 10%.

[0050] In this embodiment, the protein folding agent is glutathione or cysteine.

[0051] The culture medium used was MCDB medium.

[0052] In this embodiment, the mass ratio of component A to component B is 1:5.

[0053] In this embodiment, the mass of the degradable polypropylene film is 0.01 of the mass of the raw materials consisting of the A component and the B component.

[0054] Example 3

[0055] The wound repair membrane after endoscopic ESD surgery of the present invention is composed of component A, component B and degradable polypropylene;

[0056] Component A is composed of the following raw materials in weight percentage: animal-derived collagen, coagulation protein, snail mucus extract, protein folding agent, culture medium and green tea extract; the mass percentage of animal-derived collagen in component A is 40%, the mass percentage of coagulation protein is 22%, the mass percentage of snail mucus extract is 10%; the mass percentage of protein folding agent is 15%; and the mass percentage of green tea extract is 13%.

[0057] Component B is composed of the following raw materials in weight percentage: sodium hyaluronate gel, chitosan, conch sheath extract and oyster extract. The mass percentage of sodium hyaluronate gel in component B is 65%, the mass percentage of chitosan is 25%, the mass percentage of conch sheath extract is 5%, and the mass percentage of oyster extract is 5%.

[0058] In this embodiment, the protein folding agent is glutathione or cysteine.

[0059] The culture medium used was MCDB medium.

[0060] In this embodiment, the mass ratio of component A to component B is 1:3.

[0061] In this embodiment, the mass of the degradable polypropylene film is 0.005 of the mass of the raw materials consisting of component A and component B.

[0062] Example 4

[0063] The wound repair membrane after endoscopic ESD surgery of the present invention is composed of component A, component B and degradable polypropylene;

[0064] Component A is composed of the following raw materials in weight percentage: animal-derived collagen, coagulation protein, snail mucus extract, protein folding agent, culture medium and green tea extract; the mass percentage of animal-derived collagen in component A is 40%, the mass percentage of coagulation protein is 25%, the mass percentage of snail mucus extract is 7% to 22%; the mass percentage of protein folding agent is 8%; and the mass percentage of green tea extract is 5%.

[0065] Component B is composed of the following raw materials in weight percentage: sodium hyaluronate gel, chitosan, conch sheath extract and oyster extract. The mass percentage of sodium hyaluronate gel in component B is 40%, the mass percentage of chitosan is 20%, the mass percentage of conch sheath extract is 20%, and the mass percentage of oyster extract is 20%.

[0066] In this embodiment, the protein folding agent is glutathione or cysteine.

[0067] The culture medium used was MCDB medium.

[0068] In this embodiment, the mass ratio of component A to component B is 1:4.

[0069] In this embodiment, the mass of the degradable polypropylene film is 0.006 of the mass of the raw materials consisting of the A component and the B component.

[0070] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art may make various modifications or equivalent substitutions to the present invention within the essence and protection scope of the present invention, and such modifications or equivalent substitutions should also be deemed to fall within the protection scope of the technical solution of the present invention.

Claims

1. A wound repair membrane after endoscopic ESD surgery, characterized in that: It is composed of component A, component B and degradable polypropylene; The component A is composed of the following raw materials in percentage by weight: animal-derived collagen, coagulation protein, snail mucus extract, protein folding agent, culture medium and green tea extract; The component B is composed of the following raw materials in percentage by weight: sodium hyaluronate gel, chitosan, conch sheath extract and oyster extract; The raw materials composed of the A component and the B component are coated on a degradable polypropylene film made of degradable polypropylene to obtain an endoscopic ESD postoperative wound repair membrane.

2. The wound repair membrane after endoscopic ESD surgery according to claim 1, characterized in that: The mass ratio of the component A to the component B is 1:2-5.

3. The wound repair membrane after endoscopic ESD surgery according to claim 1, characterized in that: The mass of the degradable polypropylene film is 0.001-0.01 of the mass of the raw materials composed of the A component and the B component.

4. The wound repair membrane after endoscopic ESD surgery according to claim 1, characterized in that: The mass percentage of animal-derived collagen in component A is 25% to 40%, the mass percentage of coagulation protein is 18% to 28%, the mass percentage of snail mucus extract is 7% to 22%, the mass percentage of protein folding agent is 8% to 16%, and the mass percentage of green tea extract is 5% to 15%.

5. The wound repair membrane after endoscopic ESD surgery according to claim 1, characterized in that: The mass percentage of sodium hyaluronate gel in the B component is 40% to 65%, the mass percentage of chitosan is 20% to 45%, the mass percentage of conch sheath extract is 5% to 30%, and the mass percentage of oyster extract is 5% to 30%.

6. The wound repair membrane after endoscopic ESD surgery according to claim 1, characterized in that: The protein folding agent is glutathione or cysteine.

7. The wound repair membrane after endoscopic ESD surgery according to claim 1, characterized in that: The culture medium is MCDB culture medium.

8. The method for preparing a wound repair film after endoscopic ESD surgery according to claim 1, characterized in that: The steps include: 1) mixing the animal-derived collagen, coagulation protein, snail mucus extract and protein folding agent of component A with deionized water to obtain a mixed solution A; 2) Add culture medium and green tea extract to mixed solution A and mix well. Homogenize to obtain culture solution; 3) dissolving the sodium hyaluronate gel of component B into deionized water, adding chitosan, conch sheath extract and oyster extract and mixing evenly to obtain a mixed solution B; 4) Add mixed solution B to the culture solution in step 2) and mix well. Mix well and store at 2°C for 14-20 hours to obtain a gel; 5) Forming the degradable polypropylene into a film, and coating the gel obtained in step 4) on the degradable polypropylene film.