Treatment method of glycine fermentation liquor and application of glycine fermentation liquor in fertilizer preparation

Abstract

The invention discloses a treatment method of glycine fermentation liquor and application of the glycine fermentation liquor in fertilizer preparation, and relates to the technical field of fertilizer preparation.The treatment method comprises the following steps that firstly, the glycine fermentation liquor is introduced into a treatment container which is resistant to corrosion and good in heat preservation performance, and primary detection is conducted on the fermentation liquor firstly; the method disclosed by the invention has the advantages that by introducing a specific bio-enzyme assisted separation technology extracted by fermentation of various microorganisms and combining a novel adsorption material with high-selectivity adsorption capacity, efficient separation and resource recovery of each component in the glycine fermentation liquor are realized; according to the method, complex organic matters in the fermentation liquor can be selectively degraded by utilizing the biological enzyme, so that the complex organic matters are easier to separate, the glycine can be specifically and efficiently adsorbed by the novel adsorption material, the use of chemical reagents is reduced, the environmental pollution is greatly reduced, and the method not only effectively improves the recovery rate of the glycine, but also remarkably reduces the emission of pollutants.

Description

Technical Field

[0001] The invention relates to the technical field of fertilizer preparation, in particular to a method for processing glycine fermentation liquid and application of the method in fertilizer preparation. Background Art

[0002] As an important amino acid, glycine plays a key role in many fields, such as medicine, food, and chemical industry. It is not only a synthetic intermediate for many drugs, but also widely used in food additives, feed additives, and cosmetics. Due to its wide range of uses and market demand, the production of glycine has attracted much attention. Fermentation is currently one of the main methods for producing glycine. In the fermentation production process of glycine, the treatment of glycine fermentation broth is a crucial link, which directly affects the quality, output, and cost of the final product. The traditional glycine fermentation broth treatment method has many limitations and shortcomings; The existing method for treating glycine fermentation broth has certain defects. First, the existing technology has low efficiency in separating components of glycine fermentation broth, making it difficult to recycle resources, and uses a lot of chemical reagents, causing great environmental pollution. Second, the foam in the existing technology may affect the treatment process, resulting in low volume utilization of the fermentation broth, uneven stirring, and unstable product quality. Furthermore, when treating glycine fermentation broth, energy consumption is high, time cost is large, reaction process is slow, crystallization time is long, and production efficiency is low. Therefore, we propose a method for treating glycine fermentation broth and its application in fertilizer preparation. Summary of the invention

[0003] The purpose of the present invention is to provide a method for treating glycine fermentation broth and application of the method in fertilizer preparation.

[0004] In order to solve the problems raised in the above background technology, the present invention provides the following technical solution: a method for treating glycine fermentation broth, the method comprising the following steps: Step 1: introducing the glycine fermentation broth into a corrosion-resistant and heat-insulating treatment container, firstly conducting a preliminary test on the fermentation broth to determine its initial composition, concentration and pH parameters, adjusting the pH value of the fermentation broth, and then adding a specific biological enzyme at 5% of the volume of the fermentation broth. The specific biological enzyme is extracted by fermentation of multiple microorganisms, and the active ingredients include glucose oxidase, proteinase K and pectinase. The addition process needs to be slow and stirred continuously, and the stirring speed is adjusted to 60r / min to ensure uniform mixing; Step 2: Adjust the temperature of the treatment container to 40°C, turn on the stirring device, continue stirring at a speed of 60r / min and react for 3 hours to fully degrade the complex organic matter in the fermentation liquid. During this process, the temperature change needs to be monitored in real time. If the temperature fluctuates by more than ±1°C, adjust the heating and cooling devices in time to ensure the stability of the reaction temperature; Step 3: Add a new adsorption material with high selective adsorption capacity, the amount of which is 20 grams per liter of fermentation liquid. The adsorption material is composited with nanomaterials and has high adsorption selectivity for glycine. After adding the adsorption material, stir it using a stirring device; Step 4: Control the stirring speed to 60 r / min, continue stirring for 1 hour, and then filter through a filter device to separate the adsorption material and the fermentation liquid. When stirring for the last 10 minutes, observe the state of the fermentation liquid. When too much foam appears, add a defoamer to the fermentation liquid. The single addition amount is 0.05 ml, until the height of the foam does not exceed 10% of the height of the fermentation liquid. The amount is no more than 0.4 ml per liter of fermentation liquid, and then let it stand for a period of time; Step 5: After standing, the adsorption material is allowed to fully adsorb glycine, and then the material adsorbed with glycine is separated through a specific multi-stage filtration device to separate the adsorption material and the fermentation liquid. During the filtration process, the pressure is ensured to be stable at 0.2MPa-0.3MPa to avoid damage to the adsorption material. The fermentation liquid is then placed in a microwave field for crystallization treatment to obtain glycine crystals.

[0005] As a further scheme of the present invention: in the step 1, before adding the specific biological enzyme, when the pH value of the acid-base is not in the range of 6.8-7.2, the acid-base regulator is used to adjust the pH of the fermentation liquid to 6.8-7.2 where the specific biological enzyme activity is strongest. The acid-base regulator is selected from a 10% sodium hydroxide solution and an 8% hydrochloric acid solution. During the adjustment process, the acid-base regulator is slowly added and stirred continuously at a stirring speed of 40r / min for 30 minutes.

[0006] As a further solution of the present invention: in step three, the new adsorption material is a composite material of nano-silicon dioxide and carbon nanotubes. After adding the new adsorption material, the stirring speed is increased to 120 r / min and the stirring is continued for 30 minutes.

[0007] As a further solution of the present invention: in the step 4, after continuing stirring for 1 hour, a multi-stage filtration device is used for separation to ensure that the adsorption material and the fermentation liquid are completely separated, and the pore sizes of the filtration device are 50 μm, 20 μm and 5 μm respectively.

[0008] As a further scheme of the present invention: in the step 4, during the stirring process, the state of the fermentation liquid should be observed. If the foam exceeds 10% of the liquid level of the fermentation liquid, a defoamer is added. The defoamer is a polyether defoamer, and the amount of the defoamer is not more than 0.4 ml per liter of fermentation liquid. After stirring evenly, the standing time should be adjusted according to the concentration and temperature of the fermentation liquid. When the concentration of the fermentation liquid is 12%-15%, the standing time is 2.5 hours, when the concentration of the fermentation liquid is 10%-12%, the standing time is 2 hours, and when the concentration of the fermentation liquid is below 10%, the standing time is 1.5 hours.

[0009] As a further solution of the present invention: in the step five, a microwave-assisted treatment process is used for crystallization treatment, the microwave frequency is 2450 MHz, and the power is 500 W. In the crystallization step, the fermentation liquid after adsorption treatment is placed in a microwave field and continuously irradiated for 20 minutes to accelerate the formation of glycine crystals. During the microwave treatment, once the temperature exceeds 60°C, the microwave power is immediately reduced to 70% of the initial power, and the irradiation is continued when the temperature drops to 45°C-55°C.

[0010] As a further solution of the present invention: in the step 5, before the microwave treatment, the fermentation liquid is preheated to 45°C to improve the efficiency of the microwave treatment, and a rotary microwave reactor is used to make the fermentation liquid tumble continuously during the treatment process, with a rotation speed of 30r / min. After the microwave treatment is completed, it is quickly cooled by circulating water cooling to a temperature below 30°C to prevent the glycine crystals from re-dissolving.

[0011] As a further solution of the present invention: in step 1, when the impurity concentration in the fermentation broth exceeds 70%, the dosage of the specific biological enzyme is increased to 7% of the volume of the fermentation broth.

[0012] In addition, the present invention also provides an application of glycine fermentation broth in fertilizer preparation, and the application of the glycine fermentation broth in fertilizer preparation comprises the following specific steps: Step 1: Mix the fermentation liquid containing glycine crystals with other fertilizer components. The mixing process is carried out in a specific stirring container at a stirring speed of 40r / min to ensure that all components are fully mixed; Step 2: During the mixing process, the ratio of glycine fermentation liquid to other fertilizer components is adjusted according to different fertilizer requirements. For high-nitrogen fertilizers, the ratio of glycine fermentation liquid is reduced to 20%-30% of the total weight. For fertilizers rich in trace elements, the ratio of glycine fermentation liquid is increased to 40%-50% of the total weight. Step 3: After the mixing is completed, the fertilizer is granulated. The granulation process uses a specific granulation equipment to make the fertilizer mixture into granules with a particle size of 2mm-4mm. During the fertilizer preparation process, an analytical instrument is used to detect the glycine content, nutrient element ratio and impurity content parameters in the fertilizer to ensure that the fertilizer meets the quality standards. If the test results do not meet the standards, the proportion of the fertilizer components is adjusted and reprocessed; Step 4: Package the prepared fertilizer. The packaging material should be moisture-proof, sun-proof and breathable to ensure the quality and shelf life of the fertilizer. The ingredients, usage methods and precautions of the fertilizer should be clearly marked on the package to facilitate users to use the fertilizer correctly.

[0013] By adopting the above technical solution, compared with the prior art, the beneficial effects of the present invention are: 1. The present invention achieves efficient separation and resource recovery of various components in glycine fermentation broth by introducing a specific biological enzyme-assisted separation technology extracted by fermentation of multiple microorganisms and combining it with a new adsorption material with high selective adsorption capacity. The biological enzyme can selectively degrade complex organic matter in the fermentation broth, making it easier to separate, and the new adsorption material can efficiently adsorb glycine in a targeted manner, thereby reducing the use of chemical reagents and greatly reducing environmental pollution. This method not only effectively improves the recovery rate of glycine, but also significantly reduces the emission of pollutants; 2. The present invention uses a defoaming agent reasonably during the treatment of the glycine fermentation broth. When there is too much foam in the fermentation broth, the addition of a polyether defoaming agent can effectively eliminate the foam, thereby preventing the foam from adversely affecting the normal operation of the stirring device and subsequent treatment steps. Such control can ensure that while effectively defoaming, the foam will not occupy the volume of the fermentation broth, thereby reducing the amount of fermentation broth actually participating in the reaction and affecting the treatment effect. Excessive foam will increase the surface tension of the fermentation broth, resulting in uneven stirring, affecting the transfer of substances and the progress of the reaction. By using a defoaming agent, the stability and reliability of the treatment process can be improved, the consistency of the treatment effect can be ensured, and the product quality of glycine can be improved.

[0014] 3. The present invention accelerates the reaction process by utilizing the unique properties of microwaves, thereby reducing energy consumption and time costs in the treatment process. In the treatment process of glycine fermentation broth, microwaves can quickly stimulate the molecular movement in the fermentation broth and promote the progress of specific reactions. In particular, in the crystallization step, the formation of glycine crystals can be significantly accelerated, greatly shortening the crystallization time. At the same time, microwave heating is fast and uniform, which can effectively reduce overall energy consumption, significantly improve production efficiency and reduce costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 Schematic diagram of the extraction method steps in an embodiment of the present invention; Figure 2 It is a schematic diagram for comparing embodiments in the embodiments of the present invention. DETAILED DESCRIPTION

[0016] The specific embodiments of the present invention will be further described below in conjunction with the accompanying drawings. It should be noted that the description of these embodiments is used to help understand the present invention, but does not constitute a limitation of the present invention.

[0017] In addition, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0018] Please refer to the attached Figure 1 -Attached Figure 2 The present invention provides a method for treating a glycine fermentation broth, the method comprising the following steps: Step 1: introducing the glycine fermentation broth into a corrosion-resistant and heat-insulating treatment container, firstly conducting a preliminary test on the fermentation broth to determine its initial composition, concentration and pH parameters, adjusting the pH value of the fermentation broth, and then adding a specific biological enzyme at 5% of the volume of the fermentation broth. The specific biological enzyme is extracted by fermentation of multiple microorganisms, and the active ingredients include glucose oxidase, proteinase K and pectinase. The addition process needs to be slow and stirred continuously, and the stirring speed is adjusted to 60r / min to ensure uniform mixing; Step 2: Adjust the temperature of the treatment container to 40°C, turn on the stirring device, continue stirring at a speed of 60r / min and react for 3 hours to fully degrade the complex organic matter in the fermentation liquid. During this process, the temperature change needs to be monitored in real time. If the temperature fluctuates by more than ±1°C, adjust the heating and cooling devices in time to ensure the stability of the reaction temperature; Step 3: Add a new adsorption material with high selective adsorption capacity, the amount of which is 20 grams per liter of fermentation liquid. The adsorption material is composited with nanomaterials and has high adsorption selectivity for glycine. After adding the adsorption material, stir it using a stirring device; Step 4: Control the stirring speed to 60 r / min, continue stirring for 1 hour, and then filter through a filter device to separate the adsorption material and the fermentation liquid. When stirring for the last 10 minutes, observe the state of the fermentation liquid. When too much foam appears, add a defoamer to the fermentation liquid. The single addition amount is 0.05 ml, until the height of the foam does not exceed 10% of the height of the fermentation liquid. The amount is no more than 0.4 ml per liter of fermentation liquid, and then let it stand for a period of time; Step 5: After standing, the adsorption material is allowed to fully adsorb glycine, and then the material adsorbed with glycine is separated through a specific multi-stage filtration device to separate the adsorption material and the fermentation liquid. During the filtration process, the pressure is ensured to be stable at 0.2MPa-0.3MPa to avoid damage to the adsorption material. The fermentation liquid is then placed in a microwave field for crystallization treatment to obtain glycine crystals.

[0019] In one embodiment of the present invention: in step one, before adding the specific biological enzyme, when the pH value of the acid-base is not in the range of 6.8-7.2, an acid-base regulator is used to adjust the pH of the fermentation broth to 6.8-7.2 where the specific biological enzyme activity is strongest. The acid-base regulator is selected from a 10% sodium hydroxide solution and an 8% hydrochloric acid solution. During the adjustment process, the acid-base regulator is slowly added and stirred continuously at a stirring speed of 40 r / min for 30 minutes.

[0020] In one embodiment of the present invention: in step three, the new adsorption material is a composite material of nano-silicon dioxide and carbon nanotubes. After adding the new adsorption material, the stirring speed is increased to 120 r / min and the stirring is continued for 30 minutes.

[0021] In one embodiment of the present invention: in step 4, after continuing stirring for 1 hour, a multi-stage filtration device is used for separation to ensure that the adsorption material and the fermentation liquid are completely separated, and the pore sizes of the filtration device are 50 μm, 20 μm and 5 μm respectively.

[0022] In one embodiment of the present invention: in step 4, during the stirring process, the state of the fermentation liquid should be observed. If the foam exceeds 10% of the liquid level of the fermentation liquid, a defoamer is added. The defoamer is a polyether defoamer, and the amount of the defoamer is not more than 0.4 ml per liter of fermentation liquid. After stirring evenly, the standing time should be adjusted according to the concentration and temperature of the fermentation liquid. When the concentration of the fermentation liquid is 12%-15%, the standing time is 2.5 hours, when the concentration of the fermentation liquid is 10%-12%, the standing time is 2 hours, and when the concentration of the fermentation liquid is below 10%, the standing time is 1.5 hours.

[0023] In one embodiment of the present invention: in step five, a microwave-assisted treatment process is used for crystallization treatment, the microwave frequency is 2450 MHz, and the power is 500 W. In the crystallization step, the fermentation liquid after adsorption treatment is placed in a microwave field and continuously irradiated for 20 minutes to accelerate the formation of glycine crystals. During the microwave treatment, once the temperature exceeds 60°C, the microwave power is immediately reduced to 70% of the initial power, and the irradiation is continued when the temperature drops to 45°C-55°C.

[0024] In one embodiment of the present invention: in step five, before microwave treatment, the fermentation liquid is preheated to 45°C to improve the efficiency of microwave treatment, and a rotary microwave reactor is used to make the fermentation liquid tumble continuously during the treatment process, with a rotation speed of 30r / min. After the microwave treatment is completed, it is quickly cooled by circulating water cooling to a temperature below 30°C to prevent the glycine crystals from re-dissolving.

[0025] In one embodiment of the present invention: in step 1, when the impurity concentration in the fermentation broth exceeds 70%, the dosage of the specific biological enzyme is increased to 7% of the volume of the fermentation broth.

[0026] An application of glycine fermentation liquid in fertilizer preparation, wherein the application of glycine fermentation liquid in fertilizer preparation comprises the following specific steps: Step 1: Mix the fermentation liquid containing glycine crystals with other fertilizer components. The mixing process is carried out in a specific stirring container at a stirring speed of 40r / min to ensure that all components are fully mixed; Step 2: During the mixing process, the ratio of glycine fermentation liquid to other fertilizer components is adjusted according to different fertilizer requirements. For high-nitrogen fertilizers, the ratio of glycine fermentation liquid is reduced to 20%-30% of the total weight. For fertilizers rich in trace elements, the ratio of glycine fermentation liquid is increased to 40%-50% of the total weight. Step 3: After the mixing is completed, the fertilizer is granulated. The granulation process uses a specific granulation equipment to make the fertilizer mixture into granules with a particle size of 2mm-4mm. During the fertilizer preparation process, an analytical instrument is used to detect the glycine content, nutrient element ratio and impurity content parameters in the fertilizer to ensure that the fertilizer meets the quality standards. If the test results do not meet the standards, the proportion of the fertilizer components is adjusted and reprocessed; Step 4: Package the prepared fertilizer. The packaging material should be moisture-proof, sun-proof and breathable to ensure the quality and shelf life of the fertilizer. The ingredients, usage methods and precautions of the fertilizer should be clearly marked on the package to facilitate users to use the fertilizer correctly.

[0027] Example 1, please refer to the attached Figure 1 -Attached Figure 2, introduce the glycine fermentation broth into a corrosion-resistant and heat-insulating treatment container, conduct preliminary testing on the fermentation broth to determine its initial composition, concentration and pH parameters. When the pH value of the pH value is not within the range of 6.8-7.2, use a 10% sodium hydroxide solution and an 8% hydrochloric acid solution as acid-base regulators, slowly add and stir continuously at a speed of 40r / min for 30min to adjust the pH value of the fermentation broth to 6.8-7.2, then slowly add specific biological enzymes at 5% of the volume of the fermentation broth. When the impurity concentration in the fermentation broth exceeds 70%, increase the specific biological enzymes. The dosage is 7% of the volume of the fermentation liquid. The biological enzyme is extracted from fermentation of various microorganisms. The active ingredients include glucose oxidase, proteinase K and pectinase. During the addition process, stir continuously at a speed of 60r / min to ensure uniform mixing. Then, adjust the temperature of the treatment container to 40°C, turn on the stirring device, and continue stirring and reacting at a speed of 60r / min for 3 hours to fully degrade the complex organic matter in the fermentation liquid. During this process, if the temperature fluctuates by more than ±1°C, adjust the heating and cooling devices in time to ensure that the reaction temperature is stable at 40°C. After that, add a new adsorption material, which is nano-dioxide The material is a composite of silicon oxide and carbon nanotubes, with a dosage of 20 grams per liter of fermentation liquid. After adding, the stirring speed is increased to 120r / min, and stirring is continued for 30min. Then, the stirring speed is controlled to 60r / min, and stirring is continued for 1h. When stirring for the last 10min, the state of the fermentation liquid is observed. If the foam exceeds 10% of the fermentation liquid level, a polyether defoamer is added to the fermentation liquid. The single addition amount is 0.05ml, until the foam height does not exceed 10% of the fermentation liquid level. The defoamer dosage is no more than 0.4ml per liter of fermentation liquid. After stirring for 1h, A multi-stage filtration device with pore sizes of 50 μm, 20 μm and 5 μm was used for separation to ensure that the adsorption material and the fermentation liquid were completely separated. After stirring evenly, the standing time was adjusted according to the concentration of the fermentation liquid. When the concentration of the fermentation liquid was 12%-15%, the standing time was 2.5 hours, when the concentration of the fermentation liquid was 10%-12%, the standing time was 2 hours, and when the concentration of the fermentation liquid was below 10%, the standing time was 1.5 hours. After the standing time was completed, the adsorption material fully adsorbed glycine, and then the material adsorbed with glycine was separated through a specific multi-stage filtration device. During the filtration process, the pressure was ensured to be stable at 0.2MPa to avoid damage to the adsorption material. Subsequently, the fermentation liquid was placed in a microwave field for crystallization treatment. A microwave-assisted treatment process was used. The microwave frequency was 2450MHz and the power was 500W. During the crystallization process, the fermentation liquid after adsorption treatment was placed in a microwave field and irradiated for 20 minutes. Before microwave treatment, the fermentation liquid was preheated to 45°C. A rotary microwave reactor was used to make the fermentation liquid tumble continuously during the treatment process. The rotation speed was 30r / min. During the microwave treatment, a temperature sensor was used to monitor the temperature of the fermentation liquid in real time. Once the temperature exceeded 60°C, the microwave power was immediately reduced to 70% of the initial power. When the temperature dropped to 50°C, the irradiation continued. After the microwave treatment was completed, circulating water cooling was quickly carried out to a temperature below 30°C to prevent the glycine crystals from re-dissolving, and finally glycine crystals were obtained. .

[0028] Example 2, please refer to the attached Figure 1 -Attached Figure 2 , introduce the glycine fermentation liquid into an ordinary processing container, turn on the stirring device, and stir it continuously at a speed of 50r / min for 2.5h. Then, add the adsorption material activated carbon in an amount of 15g per liter of fermentation liquid. After adding, increase the stirring speed to 100r / min and continue stirring for 20min. Then, control the stirring speed to 50r / min and continue stirring for 0.5h. After continuing stirring for 0.5h, separate it using a multi-stage filtration device with pore sizes of 60μm, 30μm and 8μm respectively. After stirring evenly, adjust the standing time according to the concentration of the fermentation liquid. When the concentration of the fermentation liquid is 12%-15%, the standing time is 2h, when the concentration of the fermentation liquid is 10%-12%, the standing time is 1.5h, and when the concentration of the fermentation liquid is below 10%, the standing time is 1h. After standing, separate the material adsorbed with glycine through the filtration device. Subsequently, the treated fermentation liquid is simply concentrated and heated to 60°C to evaporate part of the water to increase the concentration of glycine.

[0029] Example 3, please refer to the attached Figure 1 -Attached Figure 2, introduce the glycine fermentation broth into a corrosion-resistant and heat-insulating treatment container, conduct preliminary testing on the fermentation broth to determine its initial composition, concentration and pH parameters, when the pH value of the pH value is not within the range of 6.8-7.2, use 10% sodium hydroxide solution and 8% sulfuric acid solution as acid-base regulators, slowly add and stir continuously at 40r / min for 30min, adjust the pH value of the fermentation broth to 6.8-7.2, then, slowly add specific biological enzymes at 5% of the volume of the fermentation broth, when the impurity concentration in the fermentation broth exceeds 70%, increase the dosage of the specific biological enzymes to 7% of the volume of the fermentation broth, the fermentation broth is fermented. The bioenzyme is extracted from a variety of common microorganisms by fermentation. The active ingredients include glucose oxidase and protease. During the addition process, stir continuously at a speed of 60r / min to ensure uniform mixing. Then, adjust the temperature of the treatment container to 40°C, turn on the stirring device, and continue stirring and reacting at a speed of 60r / min for 3 hours to fully degrade the complex organic matter in the fermentation liquid. During this process, if the temperature fluctuates by more than ±1°C, adjust the heating and cooling devices in time to ensure that the reaction temperature is stable at 40°C. After that, add the new adsorption material, which is a composite of nano-alumina and carbon nanofibers. The dosage is 20 grams per liter of fermentation liquid. After that, the stirring speed was increased to 120r / min, and stirring was continued for 30min. Then, the stirring speed was controlled to 60r / min, and stirring was continued for 1h. When stirring to the last 10min, the state of the fermentation liquid was observed. If the foam exceeded 10% of the fermentation liquid level, a polyether defoamer was added to the fermentation liquid. The single addition amount was 0.05ml, until the foam height did not exceed 10% of the fermentation liquid level. The defoamer dosage was no more than 0.4ml per liter of fermentation liquid. After stirring for 1h, a multi-stage filtration device with pore sizes of 50μm, 20μm and 5μm was used for separation to ensure that the adsorption material was separated from the fermentation liquid. After thorough and uniform stirring, adjust the standing time according to the concentration of the fermentation liquid. When the concentration of the fermentation liquid is 12%-15%, the standing time is 2.5h, when the concentration of the fermentation liquid is 10%-12%, the standing time is 2h, and when the concentration of the fermentation liquid is below 10%, the standing time is 1.5h. After the standing time is completed, the adsorption material is allowed to fully adsorb glycine, and then the material adsorbed with glycine is separated through a specific multi-stage filtration device. During the filtration process, ensure that the pressure is stable at 0.2-0.3MPa to avoid damage to the adsorption material. Subsequently, the treated fermentation liquid is simply concentrated and heated to 80°C to evaporate part of the water.

[0030] Take several liters of glycine fermentation broth from the same source and batch to ensure that its initial composition, concentration, pH and other parameters are basically consistent; Prepare analytical instruments for detecting the concentration and purity of glycine; The glycine fermentation broth was divided into three groups, and treated according to the methods of Example 1, Example 2 and Example 3, respectively. Each example was repeated 3 times. During the process, the extracted energy consumption data of each example was recorded in detail, including: The electrical energy consumed during heating (in kilowatt-hours); The electrical energy consumed during the mixing process (in kilowatt-hours); The electrical energy consumed during cooling (in kilowatt-hours); Electricity consumed by the operation of other related equipment (unit: kilowatt-hour); After the treatment is completed, the mass (unit: gram) and purity (%) of the glycine crystals obtained in each example are respectively measured using an analytical instrument such as a high performance liquid chromatograph, and the recovery efficiency (%) of glycine is calculated based on the content of glycine in the initial fermentation broth; The experimental results were statistically analyzed to calculate the average glycine recovery efficiency, average product purity and average extraction energy consumption of each embodiment, and the advantages and disadvantages of the three embodiments were compared; As attached Figure 2 As shown, it can be seen from the table data that Example 1 is significantly better than Example 2 and Example 3 in terms of glycine recovery efficiency and product quality, and at the same time, the extraction energy consumption is also lower, which shows that Example 1 has higher glycine recovery efficiency, better product quality, and can reduce extraction energy consumption, improve extraction efficiency, and reduce costs.

[0031] At the same time, the present application uses specific words to describe the embodiments of the present application. For example, "one embodiment", "an embodiment", and / or "some embodiments" refer to a certain feature, structure or characteristic related to at least one embodiment of the present application. Therefore, it should be emphasized and noted that "one embodiment" or "an embodiment" or "an alternative embodiment" mentioned twice or more in different positions in this specification does not necessarily refer to the same embodiment. In addition, certain features, structures or characteristics in one or more embodiments of the present application can be appropriately combined.

[0032] Similarly, it should be noted that in order to simplify the description disclosed in this application, so as to help understand one or more embodiments of the invention, in the above description of the embodiments of the present application, sometimes multiple features are merged into one embodiment, drawings or descriptions thereof. However, this disclosure method does not mean that the features required by the object of the present application are more than the features mentioned in the claims. In fact, the features of the embodiment are less than all the features of the single embodiment disclosed above. In some embodiments, numbers describing the number of components and attributes are used. It should be understood that such numbers used in the description of the embodiments are modified by the modifiers "approximately", "approximately" or "substantially" in some examples. Unless otherwise specified, "approximately", "approximately" or "substantially" indicate that the number allows a variation of ±20%. Accordingly, in some embodiments, the numerical parameters used in the specification and claims are approximate values, which may change according to the characteristics required by individual embodiments. In some embodiments, the numerical parameters should take into account the specified significant digits and adopt the general method of retaining the digits. Although the numerical domains and parameters used to confirm the breadth of their range in some embodiments of the present application are approximate values, in specific embodiments, such numerical settings are as accurate as possible within the feasible range.

[0033] Although the present invention is disclosed as above in terms of preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art may make possible changes and modifications without departing from the spirit and scope of the present invention. Therefore, any modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention without departing from the content of the technical solution of the present invention shall fall within the scope of protection defined by the claims of the present invention.

Claims

1. A method for treating a glycine fermentation broth, characterized in that: The processing method comprises the following steps: Step 1: introducing the glycine fermentation broth into a corrosion-resistant and heat-insulating treatment container, firstly conducting a preliminary test on the fermentation broth to determine its initial composition, concentration and pH parameters, adjusting the pH value of the fermentation broth, and then adding a specific biological enzyme at 5% of the volume of the fermentation broth. The specific biological enzyme is extracted by fermentation of multiple microorganisms, and the active ingredients include glucose oxidase, proteinase K and pectinase. The addition process needs to be slow and stirred continuously, and the stirring speed is adjusted to 60r / min to ensure uniform mixing; Step 2: Adjust the temperature of the treatment container to 40°C, turn on the stirring device, continue stirring at a speed of 60r / min and react for 3 hours to fully degrade the complex organic matter in the fermentation liquid. During this process, the temperature change needs to be monitored in real time. If the temperature fluctuates by more than ±1°C, adjust the heating and cooling devices in time to ensure the stability of the reaction temperature; Step 3: Add a new adsorption material with high selective adsorption capacity, the amount of which is 20 grams per liter of fermentation liquid. The adsorption material is composited with nanomaterials and has high adsorption selectivity for glycine. After adding the adsorption material, stir it using a stirring device; Step 4: Control the stirring speed to 60 r / min, continue stirring for 1 hour, and then filter through a filter device to separate the adsorption material and the fermentation liquid. When stirring for the last 10 minutes, observe the state of the fermentation liquid. When too much foam appears, add a defoamer to the fermentation liquid. The single addition amount is 0.05 ml, until the height of the foam does not exceed 10% of the height of the fermentation liquid. The amount is no more than 0.4 ml per liter of fermentation liquid, and then let it stand for a period of time; Step 5: After standing, the adsorption material is allowed to fully adsorb glycine, and then the material adsorbed with glycine is separated through a specific multi-stage filtration device to separate the adsorption material and the fermentation liquid. During the filtration process, the pressure is ensured to be stable at 0.2MPa-0.3MPa to avoid damage to the adsorption material. The fermentation liquid is then placed in a microwave field for crystallization treatment to obtain glycine crystals.

2. The method for treating a glycine fermentation broth according to claim 1, characterized in that: In the step 1, before adding the specific biological enzyme, when the pH value of the acid-base is not within the range of 6.8-7.2, an acid-base regulator is used to adjust the pH of the fermentation liquid to 6.8-7.2, where the specific biological enzyme activity is strongest. The acid-base regulator is a 10% sodium hydroxide solution and an 8% hydrochloric acid solution. During the adjustment process, the acid-base regulator is slowly added and stirred continuously at a stirring speed of 40 r / min for 30 minutes.

3. The method for treating a glycine fermentation broth according to claim 1, characterized in that: In the step three, the new adsorption material is a composite material of nano-silicon dioxide and carbon nanotubes. After adding the new adsorption material, the stirring speed is increased to 120 r / min and the stirring is continued for 30 minutes.

4. The method for treating a glycine fermentation broth according to claim 1, characterized in that: In the step 4, after continuing stirring for 1 hour, a multi-stage filtration device is used for separation to ensure that the adsorption material and the fermentation liquid are completely separated, and the pore sizes of the filtration device are 50 μm, 20 μm and 5 μm respectively.

5. The method for treating a glycine fermentation broth according to claim 1, characterized in that: In the step 4, during the stirring process, the state of the fermentation liquid should be observed. If the foam exceeds 10% of the liquid level of the fermentation liquid, a defoamer is added. The defoamer is a polyether defoamer, and the amount of the defoamer is not more than 0.4 ml per liter of fermentation liquid. After stirring evenly, the standing time should be adjusted according to the concentration and temperature of the fermentation liquid. When the concentration of the fermentation liquid is 12%-15%, the standing time is 2.5 hours, when the concentration of the fermentation liquid is 10%-12%, the standing time is 2 hours, and when the concentration of the fermentation liquid is below 10%, the standing time is 1.5 hours.

6. The method for treating a glycine fermentation broth according to claim 1, characterized in that: In the step 5, a crystallization treatment is performed using a microwave-assisted treatment process, the microwave frequency is 2450 MHz, and the power is 500 W. In the crystallization step, the fermentation liquid after the adsorption treatment is placed in a microwave field and continuously irradiated for 20 minutes to accelerate the formation of glycine crystals. During the microwave treatment, once the temperature exceeds 60°C, the microwave power is immediately reduced to 70% of the initial power, and the irradiation is continued when the temperature drops to 45°C-55°C.

7. The method for treating a glycine fermentation broth according to claim 1, characterized in that: In the step 5, before the microwave treatment, the fermentation liquid is preheated to 45° C. to improve the efficiency of the microwave treatment. A rotary microwave reactor is used to make the fermentation liquid tumble continuously during the treatment process, and the rotation speed is 30 r / min. After the microwave treatment is completed, it is quickly cooled by circulating water cooling to a temperature below 30° C. to prevent the glycine crystals from re-dissolving.

8. The method for treating a glycine fermentation broth according to claim 1, characterized in that: In the step 1, when the impurity concentration in the fermentation broth exceeds 70%, the dosage of the specific biological enzyme is increased to 7% of the volume of the fermentation broth.

9. An application of glycine fermentation broth applicable to the method for treating glycine fermentation broth according to any one of claims 1 to 8 in fertilizer preparation, characterized in that: The application of the glycine fermentation liquid in the preparation of fertilizer comprises the following specific steps: Step 1: Mix the fermentation liquid containing glycine crystals with other fertilizer components. The mixing process is carried out in a specific stirring container at a stirring speed of 40r / min to ensure that all components are fully mixed; Step 2: During the mixing process, the ratio of glycine fermentation liquid to other fertilizer components is adjusted according to different fertilizer requirements. For high-nitrogen fertilizers, the ratio of glycine fermentation liquid is reduced to 20%-30% of the total weight. For fertilizers rich in trace elements, the ratio of glycine fermentation liquid is increased to 40%-50% of the total weight. Step 3: After the mixing is completed, the fertilizer is granulated. The granulation process uses a specific granulation equipment to make the fertilizer mixture into granules with a particle size of 2mm-4mm. During the fertilizer preparation process, an analytical instrument is used to detect the glycine content, nutrient element ratio and impurity content parameters in the fertilizer to ensure that the fertilizer meets the quality standards. If the test results do not meet the standards, the proportion of the fertilizer components is adjusted and reprocessed; Step 4: Package the prepared fertilizer. The packaging material should be moisture-proof, sun-proof and breathable to ensure the quality and shelf life of the fertilizer. The ingredients, usage methods and precautions of the fertilizer should be clearly marked on the package to facilitate users to use the fertilizer correctly.

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