Gamma-glutamyl peptide as well as screening method and application thereof

Through the combination of γ-glutamyl transpeptidase enzyme synthesis and a variety of analytical technologies, γ-glutamyl peptides with good quality were screened, which solved the problem of difficulty in increasing the content of γ-glutamyl peptides in the prior art, achieved efficient and low-cost screening and synthesis, and was suitable for processing of foods with reduced salt and fat.

CN119708149AActive Publication Date: 2025-03-28ANGEL YEAST (BINZHOU) CO LTD +1
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Patent Information

Application Number
CN202510224471.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-02-27
Publication Date
2025-03-28
Estimated Expiration
2045-02-27

AI Technical Summary

Technical Problem

The prior art is difficult to effectively increase the content of γ-glutamine peptide in food, and the traditional screening method is time-consuming and costly, and cannot meet the needs of industrial production.

Method used

By providing a screening method for γ-glutamyl peptides, using γ-glutamyl transpeptidase for enzyme synthesis, combined with LC-MS/MS analysis, toxicity and water solubility analysis, and affinity analysis with 5K5S receptor proteins, γ-glutamyl peptides with better quality were screened out.

Benefits of technology

It has achieved more accurate and efficient screening to obtain better γ-glutamine peptides, which are simple to operate and have low cost, and can be synthesized by biological enzyme methods. It is suitable for improving the umami taste of food while reducing the amount of salt.

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Abstract

The invention provides gamma-glutamyl peptide as well as a screening method and application thereof. Wherein the gamma-glutamyl peptide is selected from one or more of amino acid sequences as shown in SEQ ID NO: 92, SEQ ID NO: 91, SEQ ID NO: 83, SEQ ID NO: 130, SEQ ID NO: 150, SEQ ID NO: 60, SEQ ID NO: 82, SEQ ID NO: 90 or SEQ ID NO: 93. The nine kinds of gamma-glutamyl peptides to be protected have good taste, can be simply and conveniently synthesized through a biological enzyme method, and have important significance for improving the delicate flavor of food under the condition that the use amount of salt is reduced.
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