Enrichment culture medium of aureomycin degrading bacteria and application of enrichment culture medium

Abstract

The invention relates to the technical field of wastewater biological treatment, in particular to an enrichment culture medium of aureomycin degrading bacteria and application of the enrichment culture medium. The enrichment culture medium is prepared from the following components in concentration: 10 to 15g / L of tryptone, 5 to 10g / L of yeast extract, 10 to 15g / L of NaCl, 5 to 10g / L of activated carbon, 50 to 100mg / L of aureomycin, 30 to 90mg / L of FeSO4. 7H2O, 2 to 6mL / L of trace element liquid and 2 to 6mL / L of vitamin nutrient solution. The pH value of the enrichment culture medium is 6.8 to 7.2. The aureomycin degrading bacteria obtained after enrichment culture of the enrichment culture medium provided by the invention can improve the degradation efficiency and reduce the content of aureomycin, and the system stability is strong, so that the aureomycin degrading bacteria can be used for effective treatment of aureomycin wastewater.

Description

Technical Field

[0001] The invention relates to the technical field of wastewater biological treatment, in particular to an enrichment culture medium for chlortetracycline-degrading bacteria and application thereof. Background Art

[0002] In recent years, with the rapid economic development, chlortetracycline, as a typical tetracycline antibiotic, has been widely used in animal husbandry to reduce the occurrence of respiratory diseases, promote growth, shorten feeding time, reduce feed costs, etc. However, these antibiotics are difficult to absorb after being ingested by livestock and poultry, and more than 75% of the antibiotics will be excreted with livestock and poultry feces and urine, making the concentration of chlortetracycline pollutants in livestock and poultry wastewater too high to be degraded, thus posing a huge hidden danger to environmental safety and the ecosystem.

[0003] Nowadays, the most commonly used methods for removing chlortetracycline from water are adsorption and biodegradation. The adsorption method uses a large amount of adsorption materials (adsorbents), the adsorption saturation effect will decrease, and the cost is expensive; the biological treatment method mainly relies on the physiological activities of microorganisms to treat antibiotic residues, which has the advantages of safety, low cost, and no secondary pollution. It is currently the most economical and effective treatment method.

[0004] Since the biological treatment of chlortetracycline wastewater is easily affected by factors such as substrate concentration, temperature, and pH, the degradation effect is reduced. Therefore, the bio-augmentation technology based on the addition of exogenous microorganisms has received widespread attention from scholars at home and abroad. More and more research is focused on constructing new culture media to obtain high-quality chlortetracycline-degrading bacteria, thereby improving the degradation efficiency. However, the current methods for culturing and enriching chlortetracycline-degrading bacteria are still not effective.

[0005] Therefore, it is very important to provide a solution that can solve the above technical problems. Summary of the invention

[0006] In order to solve the above problems, the purpose of the present invention is to provide an enrichment culture medium for chlortetracycline-degrading bacteria and its application. The enrichment culture medium provided by the present invention includes the following components at the following concentrations: 10-15 g / L tryptone, 5-10 g / L yeast extract, 10-15 g / L NaCl, 5-10 g / L activated carbon, 50-100 mg / L chlortetracycline, 30-90 mg / L FeSO4·7H2O, 2-6 mL / L trace element solution, 2-6 mL / L vitamin nutrient solution; the pH of the enrichment culture medium is 6.8-7.2. The chlortetracycline-degrading bacteria obtained after enrichment culture using the enrichment culture medium provided by the present invention can improve the degradation efficiency, reduce the chlortetracycline content, and the system has strong stability, which can be used for the effective treatment of chlortetracycline wastewater.

[0007] The purpose of the present invention can be achieved by the following technical solutions:

[0008] The first object of the present invention is to provide an enrichment medium for chlortetracycline-degrading bacteria, comprising the following components in the following concentrations:

[0009] 10-15 g / L tryptone, 5-10 g / L yeast extract, 10-15 g / L NaCl, 5-10 g / L activated carbon, 50-100 mg / L chlortetracycline, 30-90 mg / L FeSO4·7H2O, 2-6 mL / L trace element solution, 2-6 mL / L vitamin nutrient solution; the solvent is deionized water;

[0010] The pH of the enrichment medium is 6.8 to 7.2;

[0011] Preferably, the concentration of chlortetracycline is 50-100 mg / L.

[0012] In one embodiment of the present invention, the following concentrations of each component are included:

[0013] 10g / L tryptone, 5g / L yeast extract, 10g / L NaCl, 5g / L activated carbon, 50mg / L chlortetracycline, 60mg / L FeSO4·7H2O, 2mL / L trace element solution, 2mL / L vitamin nutrient solution; the solvent is deionized water;

[0014] The pH of the enrichment medium is 6.9.

[0015] In one embodiment of the present invention, the activated carbon is an activated carbon powder having a particle size of 1 to 150 μm;

[0016] Preferably, the particle size of the activated carbon powder is 48 μm.

[0017] In one embodiment of the present invention, the trace element solution comprises the following components in the following concentrations:

[0018] 0.1~0.15mol / L HCL, 1.0~1.8g / L FeCl2·4H2O, 0.05~0.1g / L ZnCl2, 0.05~0.12g / LMnCl2·4H2O;

[0019] Preferably, the trace element solution comprises the following components in the following concentrations:

[0020] 0.12mol / L HCL, 1.50g / L FeCl2·4H2O, 0.07g / L ZnCl2, 0.10g / L MnCl2·4H2O.

[0021] In one embodiment of the present invention, the vitamin nutrient solution comprises the following components in the following concentrations:

[0022] 30~60mg / L B1 (thiamine), 30~60mg / L B2 (riboflavin), 30~60mg / L B3 (niacin), 30~60mg / L B5 (calcium pantothenate), 90~120mg / L B6 (pyridoxine hydrochloride), 10~40mg / L B7 (biotin), 10~40mg / L B9 (folic acid), 30~60mg / L B10 (para-aminobenzoic acid), 0.5~1.5mg / L B12 (cobalamin), 30~60mg / L lipoic acid;

[0023] Preferably, the vitamin nutrient solution comprises the following components in the following concentrations:

[0024] 50mg / L B1, 50mg / L B2, 50mg / L B3, 50mg / L B5, 100mg / L B6, 20mg / L B7, 20mg / LB9, 50mg / L B10, 1mg / L B12, 50mg / L lipoic acid.

[0025] The second object of the present invention is to provide an enrichment medium for chlortetracycline-degrading bacteria and its application in enriching chlortetracycline-degrading bacteria.

[0026] The third object of the present invention is to provide a chlortetracycline-degrading bacterium obtained by enrichment culture using the enrichment medium of the above-mentioned chlortetracycline-degrading bacterium.

[0027] In one embodiment of the present invention, the chlortetracycline-degrading bacteria are prepared by the following method:

[0028] The sludge generated during the treatment of chlortetracycline wastewater is inoculated into the enrichment culture medium of chlortetracycline-degrading bacteria for enrichment culture.

[0029] In one embodiment of the present invention, the concentration of the sludge is 4000 mg / L and the inoculation amount is 5-15% (v / v);

[0030] Preferably, the inoculum amount is 10% (v / v).

[0031] In one embodiment of the present invention, during the enrichment culture, the temperature is 30-40° C. and the time is 1-2 days.

[0032] The fourth object of the present invention is to provide an application of the above-mentioned chlortetracycline-degrading bacteria in the treatment of chlortetracycline wastewater.

[0033] Compared with the prior art, the present invention has the following beneficial effects:

[0034] (1) The present invention provides an enrichment medium and an enrichment culture method to enrich and domesticate a group of highly efficient and stable chlortetracycline-degrading bacteria, which can significantly improve the removal efficiency of chlortetracycline in aquaculture wastewater.

[0035] (2) The present invention can effectively inhibit the growth of foreign bacteria in the culture medium by adding the substrate chlortetracycline to the culture medium, reduce the risk of foreign bacterial contamination, and provide a purer culture environment for the target microorganism. While inhibiting foreign bacteria, it can ensure the normal growth and metabolism of the target microorganism (chlortetracycline-degrading bacteria), which is conducive to high-quality microbial culture.

[0036] (3) The present invention can provide sufficient iron sources for microorganisms by adding FeSO4·7H2O to the culture medium to meet their iron requirements for growth and metabolism, thereby promoting the growth, reproduction and cell division of microorganisms and improving the culture efficiency and cell density of microorganisms. Iron has an important regulatory effect on the respiration, energy metabolism and activity of some key enzymes of microorganisms. Adding ferrous sulfate can improve the metabolic activity of microorganisms.

[0037] (4) The present invention adds powdered activated carbon to the culture medium. The porous structure of the powdered activated carbon provides a large number of attachment sites for microorganisms. The microorganisms can grow and reproduce on the surface and in the pores of the activated carbon, which increases the contact area between the microorganisms and nutrients, is beneficial to the uptake and utilization of nutrients by the microorganisms, and promotes the growth and reproduction of microorganisms. DETAILED DESCRIPTION

[0038] The present invention is described in detail below with reference to specific embodiments.

[0039] In the following examples, a UV-visible spectrophotometer was used to measure the concentration of aureomycin at a wavelength of 365 nm, COD was detected using the potassium dichromate method, and ammonia nitrogen was determined using the Nessler reagent spectrophotometry method.

[0040] Unless otherwise specified, all reagents used are commercially available reagents, and all detection means and methods used are conventional detection means and methods in the art.

[0041] Example 1

[0042] This embodiment provides an enrichment medium for chlortetracycline-degrading bacteria, comprising the following components at the following concentrations:

[0043] 10 g / L tryptone, 5 g / L yeast extract, 10 g / L NaCl, 5 g / L activated carbon (activated carbon powder with a particle size of 48 μm), 50 mg / L chlortetracycline, 60 mg / L FeSO4·7H2O, 2 mL / L trace element solution, 2 mL / L vitamin nutrient solution; the solvent is deionized water; the pH of the enrichment medium is 6.9;

[0044] Wherein, the trace element liquid includes the following components in concentration:

[0045] 0.12mol / L HCL, 1.50g / L FeCl2·4H2O, 0.07g / L ZnCl2, 0.10g / L MnCl2·4H2O;

[0046] The vitamin nutrient solution includes the following components in the following concentrations:

[0047] 50mg / L B1, 50mg / L B2, 50mg / L B3, 50mg / L B5, 100mg / L B6, 20mg / L B7, 20mg / LB9, 50mg / L B10, 1mg / L B12, 50mg / L lipoic acid;

[0048] Sterilize in an autoclave at 121°C for 15 minutes.

[0049] Comparative Example 1

[0050] This comparative example provides a culture medium comprising the following components at the following concentrations:

[0051] 10 g / L tryptone, 5 g / L yeast extract, 10 g / L NaCl, 5 g / L activated carbon (activated carbon powder with a particle size of 48 μm), 60 mg / L FeSO4·7H2O, 2 mL / L trace element solution, 2 mL / L vitamin nutrient solution; the solvent is deionized water; the pH of the enrichment medium is 6.9;

[0052] Wherein, the trace element liquid includes the following components in concentration:

[0053] 0.12mol / L HCL, 1.50g / L FeCl2·4H2O, 0.07g / L ZnCl2, 0.10g / L MnCl2·4H2O;

[0054] The vitamin nutrient solution includes the following components in the following concentrations:

[0055] 50mg / L B1, 50mg / L B2, 50mg / L B3, 50mg / L B5, 100mg / L B6, 20mg / L B7, 20mg / LB9, 50mg / L B10, 1mg / L B12, 50mg / L lipoic acid;

[0056] Sterilize in an autoclave at 121°C for 15 minutes.

[0057] Comparative Example 2

[0058] This comparative example provides a culture medium comprising the following components at the following concentrations:

[0059] 10g / L tryptone, 5g / L yeast extract, 10g / L NaCl, 50mg / L chlortetracycline, 60mg / LFeSO4·7H2O, 2mL / L trace element solution, 2mL / L vitamin nutrient solution; the solvent is deionized water; the pH of the enrichment medium is 6.9;

[0060] Wherein, the trace element liquid includes the following components in concentration:

[0061] 0.12mol / L HCL, 1.50g / L FeCl2·4H2O, 0.07g / L ZnCl2, 0.10g / L MnCl2·4H2O;

[0062] The vitamin nutrient solution includes the following components in the following concentrations:

[0063] 50mg / L B1, 50mg / L B2, 50mg / L B3, 50mg / L B5, 100mg / L B6, 20mg / L B7, 20mg / LB9, 50mg / L B10, 1mg / L B12, 50mg / L lipoic acid;

[0064] Sterilize in an autoclave at 121°C for 15 minutes.

[0065] Comparative Example 3

[0066] This comparative example provides a culture medium comprising the following components at the following concentrations:

[0067] 10 g / L tryptone, 5 g / L yeast extract, 10 g / L NaCl, 5 g / L activated carbon (activated carbon powder with a particle size of 48 μm), 50 mg / L chlortetracycline, 2 mL / L trace element solution, 2 mL / L vitamin nutrient solution; the solvent is deionized water; the pH of the enrichment medium is 6.9;

[0068] Wherein, the trace element liquid includes the following components in concentration:

[0069] 0.12mol / L HCL, 1.50g / L FeCl2·4H2O, 0.07g / L ZnCl2, 0.10g / L MnCl2·4H2O;

[0070] The vitamin nutrient solution includes the following components in the following concentrations:

[0071] 50mg / L B1, 50mg / L B2, 50mg / L B3, 50mg / L B5, 100mg / L B6, 20mg / L B7, 20mg / LB9, 50mg / L B10, 1mg / L B12, 50mg / L lipoic acid;

[0072] Sterilize in an autoclave at 121°C for 15 minutes.

[0073] Example 2

[0074] This embodiment provides a method for enriching and culturing chlortetracycline-degrading bacteria, which specifically includes the following steps:

[0075] The sludge produced during the treatment of chloramphenicol wastewater (concentration of 4000 mg / L, inoculation amount of 10% (V / V)) was inoculated into the enrichment culture medium of the chloramphenicol-degrading bacteria in Example 1, and then placed in a constant temperature shaker at a speed of 220 rpm and a temperature of 37°C for enrichment culture for 2 days to obtain a bacterial solution.

[0076] Comparative Example 4

[0077] This comparative example provides a method for enriching and culturing chlortetracycline-degrading bacteria, which specifically comprises the following steps:

[0078] The sludge produced during the treatment of chlortetracycline wastewater (concentration of 4000 mg / L, inoculation amount of 10% (V / V)) was inoculated into the culture medium of Comparative Example 1, and then enriched and cultured in a constant temperature shaker at a speed of 220 rpm and a temperature of 37°C for 2 days to obtain a bacterial solution.

[0079] The results showed that failure to add the substrate chlortetracycline would lead to excessive growth of foreign bacteria in the culture medium, which would be difficult to inhibit, increase the risk of foreign bacterial contamination, and affect the culture environment and quality of the target microorganisms.

[0080] Comparative Example 5

[0081] This comparative example provides a method for enriching and culturing chlortetracycline-degrading bacteria, which specifically comprises the following steps:

[0082] The sludge produced during the treatment of chlortetracycline wastewater (concentration of 4000 mg / L, inoculation amount of 10% (V / V)) was inoculated into the culture medium of Comparative Example 2, and then placed in a constant temperature shaker at a speed of 220 rpm and a temperature of 37°C for enrichment culture for 2 days to obtain a bacterial solution.

[0083] The results showed that failure to add activated carbon powder would cause microorganisms to lack attachment sites, reduce the contact area with nutrients, slow down the growth and reproduction of microorganisms, and be detrimental to the cultivation of target microorganisms.

[0084] Comparative Example 6

[0085] This comparative example provides a method for enriching and culturing chlortetracycline-degrading bacteria, which specifically comprises the following steps:

[0086] The sludge produced during the treatment of chlortetracycline wastewater (concentration of 4000 mg / L, inoculation amount of 10% (V / V)) was inoculated into the culture medium of Comparative Example 3, and then enriched and cultured in a constant temperature shaker at a speed of 220 rpm and a temperature of 37°C for 2 days to obtain a bacterial liquid of chlortetracycline-degrading bacteria.

[0087] The results showed that failure to add ferrous sulfate would lead to iron deficiency in the culture medium, affecting microbial growth, metabolism and key enzyme activity, reducing culture efficiency and resulting in reduced bacterial activity.

[0088] Therefore, the subsequent experiments further applied the bacterial solution of chlortetracycline-degrading bacteria prepared in Example 2.

[0089] Example 3

[0090] This embodiment provides an application of chlortetracycline-degrading bacteria in chlortetracycline wastewater treatment, as follows:

[0091] Experimental group: 5% (v / v1) of the chlortetracycline-degrading bacteria prepared in Example 2 was added to a 1.5L SBR reactor (v1 was 100ml activated sludge) with an initial COD concentration of 2000mg / L and an initial chlortetracycline concentration of 50mg / L.

[0092] Control group: 0% of the bacterial solution of the chlortetracycline-degrading bacteria prepared in Example 2 was added to a 1.5L SBR reactor (v1 was 100ml of activated sludge) with an initial COD concentration of 2000mg / L and an initial chlortetracycline concentration of 50mg / L;

[0093] The control group and the experimental group were subjected to degradation reaction. During the degradation reaction, an electromagnetic air pump with a power of 25W, an exhaust pressure of 0.02MPa, and an exhaust volume of 25L / min was used for aeration treatment. The water samples to be tested were taken every 6 hours to determine the concentrations of COD and chlortetracycline in the water samples.

[0094] Table 1 Removal effect of COD and chlortetracycline in sewage

[0095]

[0096] It can be found from Table 1 that the method of adding 5% (v / v1) chlortetracycline-degrading bacteria enrichment solution to the experimental group can effectively reduce the COD and chlortetracycline contents in the wastewater. The COD removal rate in the water body is 91.25%, and the chlortetracycline removal rate in the water body is 89.97%, both higher than 89.15% and 79.75% of the control group.

[0097] Example 4

[0098] This embodiment provides an application of chlortetracycline-degrading bacteria in chlortetracycline wastewater treatment, as follows:

[0099] Experimental group: 5% (v / v1) of the chlortetracycline-degrading bacteria prepared in Example 2 was added to a 1.5L SBR reactor (v1 was 100ml activated sludge) with an initial COD concentration of 4000mg / L and an initial chlortetracycline concentration of 100mg / L.

[0100] Control group: 0% of the bacterial solution of the chlortetracycline-degrading bacteria prepared in Example 2 was added to a 1.5L SBR reactor (v1 was 100ml of activated sludge) with an initial COD concentration of 4000mg / L and an initial chlortetracycline concentration of 100mg / L;

[0101] The control group and the experimental group were subjected to degradation reaction. During the degradation reaction, an electromagnetic air pump with a power of 25W, an exhaust pressure of 0.02MPa, and an exhaust volume of 25L / min was used for aeration treatment. The water samples to be tested were taken every 12h to determine the concentrations of COD and chlortetracycline in the water samples.

[0102] Table 2 Removal effect of COD and chlortetracycline in sewage

[0103]

[0104] It can be found from Table 2 that the method of adding 5% (v / v1) chlortetracycline-degrading bacteria enrichment solution to the experimental group can effectively reduce the COD and chlortetracycline contents in the wastewater. The COD removal rate in the water body is 92.88%, and the chlortetracycline removal rate in the water body is 89.93%, both higher than 89.58% and 69.97% of the control group.

[0105] Example 5

[0106] Experimental group: 10% (v / v1) of the chlortetracycline-degrading bacteria prepared in Example 2 was added to a 1.5L SBR reactor (v1 was 100ml activated sludge) with an initial ammonia nitrogen concentration of 300mg / L and an initial chlortetracycline concentration of 150mg / L.

[0107] Control group: 0% of the bacterial solution of the chlortetracycline-degrading bacteria prepared in Example 2 was added to a 1.5L SBR reactor (v1 was 100ml of activated sludge) with an initial ammonia nitrogen concentration of 300mg / L and an initial chlortetracycline concentration of 150mg / L;

[0108] The control group and the experimental group were subjected to degradation reaction. During the degradation reaction, an electromagnetic air pump with a power of 25W, an exhaust pressure of 0.02MPa, and an exhaust volume of 25L / min was used for aeration treatment. The water samples to be tested were taken every 12h to determine the concentrations of ammonia nitrogen and chlortetracycline in the water samples.

[0109] Table 3 Removal effect of ammonia nitrogen and chlortetracycline in sewage

[0110]

[0111]

[0112] It can be found from Table 3 that the method of adding an additional 10% (v / v1) chlortetracycline-degrading bacteria enrichment solution in the experimental group can effectively reduce the content of ammonia nitrogen and chlortetracycline in the wastewater. The removal rate of ammonia nitrogen in the water body is 89.21%, and the removal rate of chlortetracycline in the water body is 87.28%, both higher than 83.26% and 75.28% of the control group.

[0113] Example 6

[0114] Experimental group: 10% (v / v1) of the bacterial solution of chlortetracycline-degrading bacteria prepared in Example 2 was added to a 1.5L SBR reactor (v1 was 100ml activated sludge) with an initial ammonia nitrogen concentration of 400mg / L and an initial chlortetracycline concentration of 200mg / L.

[0115] Control group: 0% of the bacterial solution of the chlortetracycline-degrading bacteria prepared in Example 2 was added to a 1.5L SBR reactor (v1 was 100ml activated sludge) with an initial ammonia nitrogen concentration of 400mg / L and an initial chlortetracycline concentration of 20mg / L;

[0116] The control group and the experimental group were subjected to degradation reaction. During the degradation reaction, an electromagnetic air pump with a power of 25W, an exhaust pressure of 0.02MPa, and an exhaust volume of 25L / min was used for aeration treatment. The water samples to be tested were taken every 24h to determine the concentrations of ammonia nitrogen and chlortetracycline in the water samples.

[0117] Table 4 Removal effect of ammonia nitrogen and chlortetracycline in sewage

[0118]

[0119]

[0120] It can be found from Table 4 that the method of adding an additional 10% (v / v1) chlortetracycline-degrading bacteria enrichment solution in the experimental group can effectively reduce the content of ammonia nitrogen and chlortetracycline in the wastewater. The removal rate of ammonia nitrogen in the water body is 90.91%, and the removal rate of chlortetracycline in the water body is 88.81%, both higher than 85.18% and 71.59% of the control group.

[0121] Example 7

[0122] Experimental group 1: 5% (v / v1) of the bacterial solution of chlortetracycline-degrading bacteria prepared in Example 2 was added to a 1.5L SBR reactor (v1 was 100ml of activated sludge) with an initial chlortetracycline concentration of 100mg / L;

[0123] Experimental group 2: 10% (v / v1) of the bacterial solution of chlortetracycline-degrading bacteria prepared in Example 2 was added to a 1.5L SBR reactor (v1 was 100ml of activated sludge) with an initial chlortetracycline concentration of 100mg / L;

[0124] Experimental group 3: 15% (v / v1) of the bacterial solution of chlortetracycline-degrading bacteria prepared in Example 2 was added to a 1.5L SBR reactor (v1 was 100ml activated sludge) with an initial chlortetracycline concentration of 100mg / L;

[0125] Control group: 0% of the bacterial solution of the chlortetracycline-degrading bacteria prepared in Example 2 was added to a 1.5L SBR reactor (v1 was 100ml of activated sludge) with an initial chlortetracycline concentration of 100mg / L;

[0126] The control group and experimental groups (experimental group 1, experimental group 2 and experimental group 3) were subjected to degradation reaction. During the degradation reaction, an electromagnetic air pump with a power of 25W, an exhaust pressure of 0.02MPa and an exhaust volume of 25L / min was used for aeration treatment. The water samples to be tested were taken every 6 hours to determine the concentration of chlortetracycline in the water samples.

[0127] Table 5 Removal effect of chlortetracycline in sewage

[0128]

[0129]

[0130] It can be found from Table 5 that under the condition that the initial chloramphenicol concentration is 100 mg / L, considering the dosage cost and degradation effect, adding an additional 10% (v / v1) chloramphenicol degrading bacteria enriched liquid has the best comprehensive effect on reducing the chloramphenicol content in the wastewater, and the removal rate of chloramphenicol in the water is 89.99%.

[0131] The above description of the embodiments is to facilitate the understanding and use of the invention by those skilled in the art. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the present invention is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the explanation of the present invention without departing from the scope of the present invention should be within the scope of protection of the present invention.

Claims

1. An enrichment medium for chlortetracycline-degrading bacteria, characterized in that: The following concentrations of components are included: 10-15 g / L tryptone, 5-10 g / L yeast extract, 10-15 g / L NaCl, 5-10 g / L activated carbon, 50-100 mg / L chlortetracycline, 30-90 mg / L FeSO4·7H2O, 2-6 mL / L trace element solution, 2-6 mL / L vitamin nutrient solution; the solvent is deionized water; The pH of the enrichment medium is 6.8-7.

2.

2. The enrichment medium for chlortetracycline-degrading bacteria according to claim 1, characterized in that: The following concentrations of components are included: 10g / L tryptone, 5g / L yeast extract, 10g / L NaCl, 5g / L activated carbon, 50mg / L chlortetracycline, 60mg / LFeSO4·7H2O, 2mL / L trace element solution, 2mL / L vitamin nutrient solution; the solvent is deionized water; The pH of the enrichment medium is 6.

9.

3. The enrichment medium for chlortetracycline-degrading bacteria according to claim 1, characterized in that: The activated carbon is a powder with a particle size of 1 to 150 μm.

4. The enrichment medium for chlortetracycline-degrading bacteria according to claim 1, characterized in that: The trace element solution includes the following components in concentration: 0.1~0.15mol / L HCL, 1.0~1.8g / L FeCl2·4H2O, 0.05~0.1g / L ZnCl2, 0.05~0.12g / L MnCl2·4H2O.

5. The enrichment medium for chlortetracycline-degrading bacteria according to claim 1, characterized in that: The vitamin nutrient solution includes the following components in the following concentrations: 30~60mg / L B1, 30~60mg / L B2, 30~60mg / L B3, 30~60mg / L B5, 90~120mg / L B6, 10~40mg / L B7, 10~40mg / L B9, 30~60mg / L B10, 0.5~1.5mg / L B12, 30~60mg / L lipoic acid.

6. Use of the enrichment medium for chlortetracycline-degrading bacteria as claimed in any one of claims 1 to 5 in enriching chlortetracycline-degrading bacteria.

7. A chlortetracycline-degrading bacterium, characterized in that: The chlortetracycline-degrading bacteria are obtained by enrichment culture using the enrichment medium of any one of claims 1 to 5.

8. The chlortetracycline-degrading bacteria according to claim 7, characterized in that The chlortetracycline-degrading bacteria were prepared by the following method: The sludge generated during the treatment of chlortetracycline wastewater is inoculated into the enrichment culture medium of chlortetracycline-degrading bacteria for enrichment culture.

9. The chlortetracycline-degrading bacteria according to claim 8, characterized in that During the enrichment culture process, the temperature is 30-40°C and the time is 1-2 days.

10. Use of the chlortetracycline-degrading bacteria as claimed in claim 7 in the treatment of chlortetracycline wastewater.

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