Euphausia superba-derived trypsin, coding gene and application of trypsin to degradation of plasticizer
By screening and expressing highly active trypsin from Antarctic krill, the problem of low degradation efficiency of PAEs in traditional methods is solved, and the effect of efficient degradation of PAEs at low temperatures is achieved, which has important industrial application value.
Patent Information
- Application Number
- CN202510107772.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-23
- Publication Date
- 2025-03-28
- Estimated Expiration
- 2045-01-23
AI Technical Summary
The prior art is difficult to effectively degrade the plasticizers phthalate (PAEs), which are widely present in the environment, and traditional physical and chemical methods have problems of environmental protection and economic benefits.
Antarctic krill TRY gene (EsTRY) was mined through genomic analysis and heterologously expressed in E. coli. The recombinant plasmid pCold-SUMO-EsTRY was constructed to express Antarctic krill trypsin with high enzyme activity, which was used to degrade PAEs.
Antarctic krill trypsin exhibits efficient enzyme activity at low temperatures, and shows significant degradation effects on various PAEs, with a degradation rate of 98.7% within 5 hours, solving the problems of low degradation efficiency and high environmental protection costs in traditional methods.