Method for identifying wormwood germplasm resources and primer combination used by method

Abstract

The invention discloses a method for identifying wormwood germplasm resources and a primer combination used by the method. The primer combination is composed of a primer pair A.argyi. SSR1, a primer pair A.argyi. SSR4, a primer pair A.argyi. SSR5, a primer pair A.argyi. SSR7 and a primer pair A.argyi. SSR8, and the primer pair A.argyi. SSR1, the primer pair A.argyi. SSR4, the primer pair A.argyi. SSR9, the primer pair A.argyi. SSR9 and the primer pair A.argyi. Experiments prove that the primer combination provided by the invention can be used for identifying Mugi, Xiangyang mugwort, Artemisia indica, Korean mugwort, Xian'an mugwort, Inner Mongolian mugwort, Liaodong mugwort, Xinyang mugwort, bronze Sichuan mugwort, Zheng mugwort 2, Luoyang mugwort, unsealed mugwort, Hebei wild mugwort, Pingtop Mountain mugwort, three gorges mugwort, Zhou mugwort, big mugwort, Zhi mugwort, Xiangxi mugwort, Xiangxi mugwort, Xiangxi mugwort, Xiangzhou mugwort, Xiangzhou mugwort, therefore, the authenticity of 25 mugwort varieties is verified. The method has an important application value.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology, and particularly relates to a method for identifying wormwood germplasm resources and a primer combination used therein. Background Art

[0002] Mugwort is a perennial herb or slightly semi-shrubby plant of the genus Artemisia in the Asteraceae family. Mugwort is also called mugwort. The whole plant of mugwort can be used as medicine, which has the effects of warming the meridians, removing dampness, dispelling cold, antibacterial and anti-inflammatory, regulating menstruation and stopping bleeding, and enhancing immunity. It has been recorded in herbal works of various dynasties, such as "Compendium of Materia Medica" and "Shennong's Herbal Classic". Mugwort has a unique taste and is added to traditional pastries. Its young shoots and seedlings can also be eaten as vegetables. Adding mugwort leaves to ink can improve the elasticity and moisturizing properties of traditional ink, making the ink fragrant and having an antibacterial effect.

[0003] SSR molecular marker technology is a genetic marker technology based on nucleotide sequence variation in genetic material between individuals, and is a direct reflection of genetic polymorphism at the DNA level. Compared with other genetic marker technologies, SSR molecular marker technology has the characteristics of rich information, rapid and accurate detection, and easy standardized operation, making it very suitable for plant variety identification and analysis. So far, there has been no report on the application of SSR markers in the variety-specific identification of Artemisia argyi. Summary of the invention

[0004] The purpose of the present invention is how to identify the varieties of mugwort.

[0005] The present invention firstly protects a primer combination. The primer combination may include primer pair A.argyi.SSR1, primer pair A.argyi.SSR4, primer pair A.argyi.SSR5, primer pair A.argyi.SSR7 and primer pair A.argyi.SSR8; the primer pair A.argyi.SSR1 may be composed of primer A.argyi.SSR1-F with a nucleotide sequence as shown in SEQ ID NO:1 and primer A.argyi.SSR1-F with a nucleotide sequence as shown in SEQ ID NO:2.

[0006] A.argyi.SSR1-R; the primer pair A.argyi.SSR4 may be composed of a primer with a nucleotide sequence as shown in SEQ ID NO:3

[0007] A.argyi.SSR4-F and a primer A.argyi.SSR4-R having a nucleotide sequence as shown in SEQ ID NO:4; the primer pair

[0008] A.argyi.SSR5 can be composed of primer A.argyi.SSR5-F having a nucleotide sequence as shown in SEQ ID NO:5 and primer A.argyi.SSR5-R having a nucleotide sequence as shown in SEQ ID NO:6; the primer pair A.argyi.SSR7 can be composed of primer A.argyi.SSR7-F having a nucleotide sequence as shown in SEQ ID NO:7 and primer A.argyi.SSR7-R having a nucleotide sequence as shown in SEQ ID NO:8; the primer pair A.argyi.SSR8 can be composed of primer A.argyi.SSR8-F having a nucleotide sequence as shown in SEQ ID NO:9 and primer A.argyi.SSR8-R having a nucleotide sequence as shown in SEQ ID NO:10.

[0009] In the primer combination, the 5' ends of primer A.argyi.SSR1-F, primer A.argyi.SSR4-F, primer A.argyi.SSR5-F, primer A.argyi.SSR7-F and primer A.argyi.SSR8-F can be modified with different fluorescent groups. The primer combination composed of primers modified with different fluorescent groups is used to detect PCR amplification products by capillary electrophoresis.

[0010] In an embodiment of the present invention, primer A.argyi.SSR1-F can be modified with a ROX fluorescent group, and the fluorescent color emitted is red. Primer A.argyi.SSR4-F can be modified with a TAMRA fluorescent group, and the fluorescent color emitted is black. Primer A.argyi.SSR5-F can be modified with a HEX fluorescent group, and the fluorescent color emitted is green. Primer A.argyi.SSR7-F can be modified with a FAM fluorescent group, and the fluorescent color emitted is blue. Primer A.argyi.SSR8-F can be modified with a TAMRA fluorescent group, and the fluorescent color emitted is black.

[0011] Any of the above-mentioned primer combinations can specifically be composed of any of the above-mentioned primer pairs A.argyi.SSR1, any of the above-mentioned primer pairs A.argyi.SSR4, any of the above-mentioned primer pairs A.argyi.SSR5, any of the above-mentioned primer pairs A.argyi.SSR7 and any of the above-mentioned primer pairs A.argyi.SSR8.

[0012] The use of any of the above-mentioned primer combinations in identifying Wan mugwort, Xiangyang mugwort, May mugwort, Korean mugwort, Xi'an mugwort, Inner Mongolia mugwort, Liaodong mugwort, Xinyang mugwort, Tongchuan mugwort, Zheng mugwort No. 2, Luoyang mugwort, Kaifeng mugwort, Hebei wild mugwort, Pingdingshan mugwort, Sanmenxia mugwort, Zhoukou mugwort, big mugwort, Zhumadian mugwort, Tangyin mugwort, Hunan and Jiangxi mugwort, Suizhou mugwort, Central and South mugwort, Sichuan mugwort, Xiaogan mugwort and red-footed mugwort also falls within the scope of protection of the present invention.

[0013] The present invention also protects a method for identifying whether the mugwort to be tested is or is a candidate for being Wan mugwort, Xiangyang mugwort, May mugwort, Korean mugwort, Xi'an mugwort, Inner Mongolia mugwort, Liaodong mugwort, Xinyang mugwort, Tongchuan mugwort, Zheng mugwort No. 2, Luoyang mugwort, Kaifeng mugwort, Hebei wild mugwort, Pingdingshan mugwort, Sanmenxia mugwort, Zhoukou mugwort, big mugwort, Zhumadian mugwort, Tangyin mugwort, Xianggan mugwort, Suizhou mugwort, Central and South mugwort, Sichuan mugwort, Xiaogan mugwort and red-footed mugwort, which can be specifically method one, and can include the following steps: using the genomic DNA of the mugwort to be tested as a template, respectively using any of the above-mentioned primer pairs A.argyi.SSR1, any of the above-mentioned primer pairs A.argyi.SSR4, any of the above-mentioned primer pairs A.argyi.SSR5, any of the above-mentioned primer pairs A.argy i. SSR7 and any of the above primer pairs A. argyi. SSR8 are used for PCR amplification to obtain PCR amplification products; then the following judgment is made: if primer pair A. argyi. SSR1 is used to amplify to obtain amplification products of 150 bp, 161 bp, 166 bp and 170 bp, primer pair A. argyi. SSR4 is used to amplify to obtain amplification products of 139 bp, 154 bp and 157 bp, primer pair A. argyi. SSR5 is used to amplify to obtain amplification products of 138 bp and 142 bp, primer pair A. argyi. SSR7 is used to amplify to obtain amplification products of 128 bp and 137 bp, and primer pair A. argyi. SSR8 is used to amplify to obtain amplification products of 138 bp and 142 bp, and primer pair A. argyi. SSR9 is used to amplify to obtain amplification products of 128 bp and 137 bp, and primer pair A. If the amplification product of 128 bp can be obtained by amplification with primer pair A.argyi.SSR8, then the wormwood to be tested is or the candidate is Wan Ai; if the amplification products of 161 bp and 170 bp can be obtained by amplification with primer pair A.argyi.SSR1, the amplification products of 139 bp, 148 bp and 157 bp can be obtained by amplification with primer pair A.argyi.SSR4, the amplification products of 133 bp and 136 bp can be obtained by amplification with primer pair A.argyi.SSR5, the amplification products of 128 bp and 137 bp can be obtained by amplification with primer pair A.argyi.SSR7, and the amplification product of 128 bp can be obtained by amplification with primer pair A.argyi.SSR8, then the wormwood to be tested is or candidate is Xiangyang mugwort; if primer pair A.argyi.SSR1 is used to amplify to obtain amplification products of 150bp, 154bp and 161bp, primer pair A.argyi.SSR4 is used to amplify to obtain amplification products of 139bp, 145bp and 154bp, primer pair A.argyi.SSR5 is used to amplify to obtain amplification products of 133bp and 136bp, primer pair A.argyi.SSR7 is used to amplify to obtain amplification products of 119bp and 137bp, and primer pair A.argyi.SSR8 is used to amplify to obtain amplification products of 128bp, then the mugwort to be tested is or candidate is May mugwort; if primer pair A.argyi.SSR1 amplification can obtain amplification products of 161bp, 166bp and 170bp, primer pair A.argyi.SSR4 amplification can obtain amplification products of 139bp and 145bp, primer pair A.argyi.SSR5 amplification can obtain amplification products of 140bp and 148bp, primer pair A.argyi.SSR7 amplification can obtain amplification products of 128bp and 137bp, and primer pair A.argyi.SSR8 amplification can obtain amplification products of 128bp, then the mugwort to be tested is or is a candidate for Korean mugwort; if primer pair A.argyi.SSR1 amplification can obtain amplification products of 170bp, primer pair A.argyi. If SSR4 amplification can obtain an amplification product of 157 bp, primer pair A.argyi.SSR5 amplification can obtain an amplification product of 140 bp, primer pair A.argyi.SSR7 amplification can obtain an amplification product of 137 bp, and primer pair A.argyi.SSR8 amplification can obtain an amplification product of 128 bp, then the mugwort to be tested is or the candidate is Xi'an mugwort; if primer pair A.argyi.SSR1 amplification can obtain amplification products of 161 bp and 170 bp, primer pair A.argyi.SSR4 amplification can obtain amplification products of 139 bp and 154 bp, and primer pair A.argyi.SSR5 amplification can obtain amplification products of 136 bp and 14 If the amplification product of 0bp is obtained by using primer pair A.argyi.SSR7, and the amplification product of 128bp is obtained by using primer pair A.argyi.SSR8, then the mugwort to be tested is or the candidate is Inner Mongolia mugwort; if the amplification products of 161bp and 170bp are obtained by using primer pair A.argyi.SSR1, the amplification products of 139bp, 145bp, 148bp and 154bp are obtained by using primer pair A.argyi.SSR4, the amplification products of 136bp, 140bp and 142bp are obtained by using primer pair A.argyi.SSR5, and the amplification products of 136bp, 140bp and 142bp are obtained by using primer pair A.argyi.SSR7. If amplification products of 128 bp, 137 bp and 154 bp can be obtained and amplification product of 128 bp can be obtained by using primer pair A.argyi.SSR8, then the mugwort to be tested is or is a candidate for Artemisia liaotungensis; if amplification products of 161 bp and 170 bp can be obtained by using primer pair A.argyi.SSR1, amplification products of 139 bp and 154 bp can be obtained by using primer pair A.argyi.SSR4, amplification products of 140 bp and 142 bp can be obtained by using primer pair A.argyi.SSR5, amplification products of 119 bp, 137 bp and 140 bp can be obtained by using primer pair A.argyi.SSR7, and amplification products of 119 bp, 137 bp and 140 bp can be obtained by using primer pair A.argyi.SSR8.argyi.SSR8 amplification can obtain an amplification product of 128bp, then the wormwood to be tested is or the candidate is Xinyang wormwood; if the primer pair A.argyi.SSR1 amplification can obtain amplification products of 161bp, 170bp and 175bp, the primer pair A.argyi.SSR4 amplification can obtain amplification products of 139bp and 148bp, the primer pair A.argyi.SSR5 amplification can obtain amplification products of 140bp and 142bp, the primer pair A.argyi.SSR7 amplification can obtain amplification products of 137bp, and the primer pair A.argyi.SSR8 amplification can obtain amplification products of 128bp and 132bp, then the wormwood to be tested is or candidate is Tongchuan mugwort; if primer pair A.argyi.SSR1 is used to amplify to obtain amplification products of 161bp and 170bp, primer pair A.argyi.SSR4 is used to amplify to obtain amplification products of 139bp, 148bp, 154bp and 157bp, primer pair A.argyi.SSR5 is used to amplify to obtain amplification products of 133bp and 136bp, primer pair A.argyi.SSR7 is used to amplify to obtain amplification products of 137bp and 154bp, and primer pair A.argyi.SSR8 is used to amplify to obtain amplification products of 128bp, then the mugwort to be tested is or candidate is Zheng Ai No. 2; if primer pair A.argyi.SSR 1 amplification can obtain amplification products of 161bp, 166bp and 170bp, using primer pair A.argyi.SSR4 amplification can obtain amplification products of 139bp, 145bp, 148bp and 154bp, using primer pair A.argyi.SSR5 amplification can obtain amplification products of 133bp, 136bp and 140bp, using primer pair A.argyi.SSR7 amplification can obtain amplification products of 119bp, 137bp, 140bp and 154bp, and using primer pair A.argyi.SSR8 amplification can obtain an amplification product of 128bp, then the mugwort to be tested is or the candidate is Luoyang mugwort; if primer pair A.argyi.SSR1 is used for amplification Amplification products of 150 bp, 161 bp and 170 bp can be obtained by using primer pair A.argyi.SSR4, amplification products of 145 bp, 148 bp and 154 bp can be obtained by using primer pair A.argyi.SSR5, amplification products of 136 bp, 140 bp and 148 bp can be obtained by using primer pair A.argyi.SSR7, amplification products of 128 bp can be obtained by using primer pair A.argyi.SSR8, and amplification products of 128 bp can be obtained by using primer pair A.argyi.SSR1. If amplification products of 161 bp and 170 bp can be obtained by using primer pair A.argyi.SSR2, amplification products of 161 bp and 170 bp can be obtained by using primer pair A.argyi.SSR3, and amplification products of 136 bp, 140 bp and 148 bp can be obtained by using primer pair A.argyi.SSR4, and amplification products of 136 bp, 140 bp and 148 bp can be obtained by using primer pair A.argyi.SSR5, and amplification products of 128 bp can be obtained by using primer pair A.argyi.SSR7, and amplification products of 128 bp can be obtained by using primer pair A.argyi.SSR8, then the mugwort to be tested is or the candidate is Kaifeng mugwort; if amplification products of 161 bp and 170 bp can be obtained by using primer pair A.If the primer pair A.argyi.SSR4 can amplify products of 139 bp, 148 bp and 154 bp, the primer pair A.argyi.SSR5 can amplify products of 133 bp, 136 bp and 140 bp, the primer pair A.argyi.SSR7 can amplify products of 128 bp and the primer pair A.argyi.SSR8 can amplify products of 128 bp, then the mugwort to be tested is or is the candidate for Hebei wild mugwort; if the primer pair A.argyi.SSR1 can amplify products of 161 bp, 170 bp and 175 bp, the primer pair A.argyi.SSR4 can amplify products of 139 bp, If the amplification products of primer pair A.argyi.SSR5 can be obtained, the amplification products of 133bp, 136bp and 140bp can be obtained, the amplification products of primer pair A.argyi.SSR7 can be obtained, and the amplification product of 128bp can be obtained by amplification with primer pair A.argyi.SSR8, then the mugwort to be tested is or the candidate is Pinus mugwort; if the amplification products of primer pair A.argyi.SSR1 can be obtained, the amplification products of 161bp and 170bp can be obtained, the amplification products of primer pair A.argyi.SSR4 can be obtained, and the amplification products of primer pair A.argyi.SSR5 can be obtained, the amplification products of 119bp, 128bp and 137bp can be obtained, and the amplification product of primer pair A.argyi.SSR8 can be obtained, then the mugwort to be tested is or the candidate is Pinus mugwort; if the amplification products of primer pair A.argyi.SSR1 can be obtained, the amplification products of primer pair A.argyi.SSR4 can be obtained, and the amplification products of primer pair A.argyi.S SR5 amplification can obtain amplification products of 133bp, 140bp and 142bp, and primer pair A.argyi.SSR7 amplification can obtain an amplification product of 128bp and primer pair A.argyi.SSR8 amplification can obtain an amplification product of 128bp, then the mugwort to be tested is or the candidate is Sanmenxia mugwort; if primer pair A.argyi.SSR1 amplification can obtain an amplification product of 166bp, primer pair A.argyi.SSR4 amplification can obtain amplification products of 142bp and 145bp, primer pair A.argyi.SSR5 amplification can obtain amplification products of 136bp and 140bp, and primer pair A.argyi.SSR 7 can obtain amplification products of 119 bp and 140 bp and primer pair A.argyi.SSR8 can obtain amplification products of 110 bp and 128 bp, then the mugwort to be tested is or the candidate is Zhoukou mugwort; if primer pair A.argyi.SSR1 can obtain amplification products of 170 bp, primer pair A.argyi.SSR4 can obtain amplification products of 142 bp and 145 bp, primer pair A.argyi.SSR5 can obtain amplification products of 136 bp and 152 bp, primer pair A.argyi.SSR7 can obtain amplification products of 119 bp and 137 bp and primer pair A.argyi.SSR8 amplification can obtain an amplification product of 110bp, then the wormwood to be tested is or the candidate is Artemisia argyi; if the primer pair A.argyi.SSR1 amplification can obtain amplification products of 150bp, 166bp and 170bp, the primer pair A.argyi.SSR4 amplification can obtain amplification products of 139bp and 148bp, the primer pair A.argyi.SSR5 amplification can obtain amplification products of 124bp, 133bp and 140bp, the primer pair A.argyi.SSR7 amplification can obtain an amplification product of 137bp, and the primer pair A.argyi.SSR8 amplification can obtain an amplification product of 128bp, then the wormwood to be tested is or the candidate is Artemisia argyi. Madian mugwort; if primer pair A.argyi.SSR1 is used to amplify to obtain amplification products of 161bp, 166bp and 170bp, primer pair A.argyi.SSR4 is used to amplify to obtain amplification products of 139bp and 145bp, primer pair A.argyi.SSR5 is used to amplify to obtain amplification products of 124bp, 133bp, 136bp, 140bp and 142bp, primer pair A.argyi.SSR7 is used to amplify to obtain amplification product of 137bp, and primer pair A.argyi.SSR8 is used to amplify to obtain amplification product of 128bp, then the mugwort to be tested is or the candidate is Tangyin mugwort; if primer pair A.argyi.S Amplification with primer pair A.argyi.SSR1 can obtain an amplification product of 170bp, amplification with primer pair A.argyi.SSR4 can obtain amplification products of 139bp, 145bp and 154bp, amplification with primer pair A.argyi.SSR5 can obtain amplification products of 133bp, 136bp and 140bp, amplification with primer pair A.argyi.SSR7 can obtain an amplification product of 137bp, and amplification with primer pair A.argyi.SSR8 can obtain an amplification product of 128bp, then the mugwort to be tested is or the candidate is Xianggan mugwort; if amplification with primer pair A.argyi.SSR1 can obtain amplification products of 161bp, 166bp and 170bp, and amplification with primer pair A.argyi.SSR4 can obtain amplification products of 139bp, 145bp and 148bp, primer pair A.argyi.SSR5 can obtain amplification products of 133bp, 136bp and 142bp, primer pair A.argyi.SSR7 can obtain amplification products of 119bp, 128bp, 137bp and 154bp, and primer pair A.argyi.SSR8 can obtain amplification products of 128bp, then the mugwort to be tested is or the candidate is Suizhou mugwort; if primer pair A.argyi.SSR1 can obtain amplification products of 161bp, 166bp and 170bp, primer pair A.argyi.If SSR4 amplification can obtain amplification products of 139bp and 145bp, primer pair A.argyi.SSR5 amplification can obtain amplification products of 133bp and 136bp, primer pair A.argyi.SSR7 amplification can obtain amplification products of 119bp, 137bp and 154bp, and primer pair A.argyi.SSR8 amplification can obtain amplification products of 120bp and 128bp, then the mugwort to be tested is or the candidate is central and southern mugwort; if primer pair A.argyi.SSR1 is used to amplify, 154bp, 161bp , 166bp and 170bp amplification products can be obtained by amplifying with primer pair A.argyi.SSR4, 139bp and 145bp amplification products can be obtained by amplifying with primer pair A.argyi.SSR5, 133bp, 136bp and 142bp amplification products can be obtained by amplifying with primer pair A.argyi.SSR7, 119bp and 140bp amplification products can be obtained by amplifying with primer pair A.argyi.SSR8, and 128bp amplification products can be obtained by amplifying with primer pair A.argyi.SSR9, then the mugwort to be tested is or the candidate is Artemisia argyi; if the primer pair A.argyi is used i. If the amplification of SSR1 can obtain amplification products of 161bp and 166bp, the amplification of primer pair A.argyi.SSR4 can obtain amplification products of 142bp and 154bp, the amplification of primer pair A.argyi.SSR5 can obtain amplification products of 133bp and 140bp, the amplification of primer pair A.argyi.SSR7 can obtain amplification products of 137bp and 140bp, and the amplification of primer pair A.argyi.SSR8 can obtain amplification products of 128bp, then the mugwort to be tested is or the candidate is Xiaogan mugwort; if primer pair Amplification of A.argyi.SSR1 can obtain amplification products of 161bp and 170bp, amplification of A.argyi.SSR4 can obtain amplification products of 139bp and 145bp, amplification of A.argyi.SSR5 can obtain amplification products of 136bp, 138bp and 142bp, amplification of A.argyi.SSR7 can obtain amplification products of 119bp, and amplification of A.argyi.SSR8 can obtain amplification products of 128bp, then the mugwort to be tested is or is a candidate for red-footed mugwort. .

[0014] The present invention also protects a method for identifying whether the mugwort to be tested is or is a candidate for Wan mugwort, Xiangyang mugwort, May mugwort, Korean mugwort, Xi'an mugwort, Inner Mongolia mugwort, Liaodong mugwort, Xinyang mugwort, Tongchuan mugwort, Zheng mugwort No. 2, Luoyang mugwort, Kaifeng mugwort, Hebei wild mugwort, Pingdingshan mugwort, Sanmenxia mugwort, Zhoukou mugwort, big mugwort, Zhumadian mugwort, Tangyin mugwort, Xianggan mugwort, Suizhou mugwort, Central and South mugwort, Sichuan mugwort, Xiaogan mugwort and red-footed mugwort, which can be specifically method 2, and can include the following steps:

[0015] (1) Using the genomic DNA of the mugwort to be tested as a template, PCR amplification is performed using any of the above primer pairs A.argyi.SSR1, any of the above primer pairs A.argyi.SSR4, any of the above primer pairs A.argyi.SSR5, any of the above primer pairs A.argyi.SSR7, and any of the above primer pairs A.argyi.SSR8 to obtain a PCR amplification product;

[0016] (2) using the genomic DNA of Wan mugwort, Xiangyang mugwort, May mugwort, Korean mugwort, Xi'an mugwort, Inner Mongolia mugwort, Liaodong mugwort, Xinyang mugwort, Tongchuan mugwort, Zheng mugwort No. 2, Luoyang mugwort, Kaifeng mugwort, Hebei wild mugwort, Pingdingshan mugwort, Sanmenxia mugwort, Zhoukou mugwort, big mugwort, Zhumadian mugwort, Tangyin mugwort, Xianggan mugwort, Suizhou mugwort, Central and South mugwort, Sichuan mugwort, Xiaogan mugwort and red-foot mugwort as templates, and performing PCR amplification using any of the above-mentioned primer pairs A.argyi.SSR1, any of the above-mentioned primer pairs A.argyi.SSR4, any of the above-mentioned primer pairs A.argyi.SSR5, any of the above-mentioned primer pairs A.argyi.SSR7 and any of the above-mentioned primer pairs A.argyi.SSR8 to obtain PCR amplification products;

[0017] (3) Make the following judgment: if the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Wan mugwort, then the mugwort to be tested is or the candidate is Wan mugwort; if the mugwort to be tested is If the PCR amplification products obtained by respectively using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for mugwort are consistent in size with the PCR amplification products obtained by respectively using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Xiangyang mugwort, then the mugwort to be tested is or the candidate is Xiangyang mugwort; if the mugwort to be tested is respectively using the primer pair A .argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 are all the same size as the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Artemisia seu Lour., then the Artemisia seu Lour. is or is a candidate for Artemisia seu Lour.; if the Artemisia seu Lour. is respectively obtained by using the primer pairs A.argyi.SSR1, The PCR amplification products obtained by the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 are all consistent in size with the PCR amplification products obtained by the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 of Korean mugwort, respectively. Then the mugwort to be tested is or is a candidate for Korean mugwort; if the mugwort to be tested is respectively obtained by the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 are all the same size as the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 of Xi'an mugwort, then the mugwort to be tested is or is a candidate for Xi'an mugwort; if the mugwort to be tested is respectively using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 rgyi.SSR5, the PCR amplification products obtained by the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Inner Mongolia mugwort, then the mugwort to be tested is or is a candidate for Inner Mongolia mugwort; if the mugwort to be tested is respectively using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 The PCR amplification products obtained for A.argyi.SSR7 and the primer pair A.argyi.SSR8 are consistent in size with the PCR amplification products obtained for Artemisia liaotungensis using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8, respectively, then the wormwood to be tested is or is a candidate for Artemisia liaotungensis; if the wormwood to be tested uses the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 are all the same size as the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 of Xinyang mugwort, then the mugwort to be tested is or is a candidate for Xinyang mugwort; if the mugwort to be tested is respectively using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.If the PCR amplification products obtained by SSR8 are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Tongchuan mugwort, then the mugwort to be tested is or is a candidate for Tongchuan mugwort; if the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Tongchuan mugwort are respectively consistent in size, then the mugwort to be tested is or is a candidate for Tongchuan mugwort; If the sizes of the PCR amplification products of the tested wormwoods are consistent with those of the wormwoods of Zheng Ai No. 2 respectively obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8, then the wormwood to be tested is or is the candidate for Zheng Ai No. 2; if the PCR amplification products of the tested wormwoods respectively obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 are consistent with those of the wormwoods of Luoyang respectively obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 The PCR amplification products obtained by the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 are of the same size, then the mugwort to be tested is or the candidate is Luoyang mugwort; if the PCR amplification products obtained by the mugwort to be tested using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 are all the same size as the PCR amplification products obtained by the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8, respectively, of Kaifeng mugwort using the primer pair A.argyi.S SR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 have the same size of PCR amplification products, then the tested mugwort is or the candidate is Kaifeng mugwort; if the PCR amplification products obtained by the tested mugwort using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 are all the same as the PCR amplification products obtained by the wild mugwort in Hebei using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8, respectively.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 have the same size of PCR amplification products, then the mugwort to be tested is or the candidate is Hebei wild mugwort; if the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 to be tested are the same as the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 to be tested are the same as the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 to be tested, respectively. R4, the PCR amplification products obtained by the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 are of the same size, then the mugwort to be tested is or is a candidate for Pingdingshan mugwort; if the PCR amplification products obtained by the mugwort to be tested using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 are all the same as the PCR amplification products obtained by the Sanmenxia mugwort using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8, respectively. argyi.SSR5, the PCR amplification products obtained by the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 are of the same size, then the mugwort to be tested is or the candidate is Sanmenxia mugwort; if the PCR amplification products obtained by the mugwort to be tested using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 are the same as the PCR amplification products obtained by Zhoukou mugwort using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, The PCR amplification products obtained by the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 are of the same size, then the mugwort to be tested is or is a candidate for Zhoukou mugwort; if the PCR amplification products obtained by the mugwort to be tested using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 are all the same as the PCR amplification products obtained by the mugwort to be tested using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8, respectively.SSR7 and the PCR amplification products obtained by the primer pair A.argyi.SSR8 are consistent in size, then the mugwort to be tested is or the candidate is large mugwort; if the PCR amplification products obtained by the mugwort to be tested using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 are consistent in size, then the mugwort to be tested is or the candidate is large mugwort; if the PCR amplification products obtained by the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 are consistent in size with the PCR amplification products obtained by the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 of Zhumadian mugwort and the size of the PCR amplification product obtained by the primer pair A.argyi.SSR8, then the mugwort to be tested is or the candidate is Zhumadian mugwort; if the PCR amplification products obtained by the mugwort to be tested using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 are all the same as the PCR amplification products obtained by the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 of Tangyin mugwort, respectively. If the size of the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 are consistent with the size of the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 of the tested mugwort are consistent with the size of the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi. rgyi.SSR8, the PCR amplification products obtained are consistent in size, then the tested mugwort is or is a candidate for Xianggan mugwort; if the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the tested mugwort are consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.If the size of the PCR amplification products obtained by SSR8 is consistent, the mugwort to be tested is or the candidate is Suizhou mugwort; if the PCR amplification products obtained by the mugwort to be tested using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 are consistent with the PCR amplification products obtained by the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 of Zhongnan mugwort, respectively If the sizes of the PCR amplification products are consistent, the mugwort to be tested is or is a candidate for Central and Southern mugwort; if the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are the same in size as the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Sichuan mugwort If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the wormwood to be tested are consistent with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Xiaogan wormwood, the wormwood to be tested is or the candidate is Sichuan wormwood. If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Xiaogan wormwood are consistent in size, then the wormwood to be tested is Or the candidate is Xiaogan mugwort; if the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all the same size as the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for red-footed mugwort, then the mugwort to be tested is or the candidate is red-footed mugwort. .

[0018] In any of the above methods, the PCR amplification product can be detected by electrophoresis. Preferably, the electrophoresis can be capillary electrophoresis. The electrophoresis can also be agarose gel electrophoresis. The gel concentration of the agarose gel electrophoresis can be 1%-3% (v / v).

[0019] The present invention also protects a kit containing any of the above primer combinations; the purpose of the kit is to identify Wan mugwort, Xiangyang mugwort, May mugwort, Korean mugwort, Xi'an mugwort, Inner Mongolia mugwort, Liaodong mugwort, Xinyang mugwort, Tongchuan mugwort, Zheng mugwort No. 2, Luoyang mugwort, Kaifeng mugwort, Hebei wild mugwort, Pingdingshan mugwort, Sanmenxia mugwort, Zhoukou mugwort, big mugwort, Zhumadian mugwort, Tangyin mugwort, Xianggan mugwort, Suizhou mugwort, Zhongnan mugwort, Sichuan mugwort, Xiaogan mugwort and red-footed mugwort. The present invention also protects a method for preparing the kit. The method for preparing the kit may include the step of separately packaging each primer in any of the above primer combinations.

[0020] The use of any of the above-mentioned kits in identifying Wan mugwort, Xiangyang mugwort, May mugwort, Korean mugwort, Xi'an mugwort, Inner Mongolia mugwort, Liaodong mugwort, Xinyang mugwort, Tongchuan mugwort, Zheng mugwort No. 2, Luoyang mugwort, Kaifeng mugwort, Hebei wild mugwort, Pingdingshan mugwort, Sanmenxia mugwort, Zhoukou mugwort, big mugwort, Zhumadian mugwort, Tangyin mugwort, Hunan and Jiangxi mugwort, Suizhou mugwort, Central and South mugwort, Sichuan mugwort, Xiaogan mugwort and red-footed mugwort also falls within the scope of protection of the present invention.

[0021] Experiments have shown that the primer combination provided by the present invention can be used to identify Wan mugwort, Xiangyang mugwort, May mugwort, Korean mugwort, Xi'an mugwort, Inner Mongolia mugwort, Liaodong mugwort, Xinyang mugwort, Tongchuan mugwort, Zheng mugwort No. 2, Luoyang mugwort, Kaifeng mugwort, Hebei wild mugwort, Pingdingshan mugwort, Sanmenxia mugwort, Zhoukou mugwort, big mugwort, Zhumadian mugwort, Tangyin mugwort, Xianggan mugwort, Suizhou mugwort, Central and South mugwort, Sichuan mugwort, Xiaogan mugwort and red-footed mugwort, thereby verifying the authenticity of 25 mugwort varieties. The present invention has important application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 The results of 1% agarose gel testing of genomic DNA of some Artemisia species.

[0023] Figure 2 Part of the agarose gel electrophoresis results of the PCR amplification products obtained by using primer pair 1 to amplify 25 mugwort varieties.

[0024] Figure 3 Part of the agarose gel electrophoresis results of the PCR amplification products obtained by using primer pair 4 to amplify 25 mugwort varieties.

[0025] Figure 4 Part of the agarose gel electrophoresis results of the PCR amplification products obtained by using primer pair 5 to amplify 25 mugwort varieties.

[0026] Figure 5Part of the agarose gel electrophoresis results of the PCR amplification products obtained by using primer pair 7 to amplify 25 mugwort varieties.

[0027] Figure 6 Part of the agarose gel electrophoresis results of the PCR amplification products obtained by using primer pair 8 to amplify 25 mugwort varieties.

[0028] Figure 7 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying A.argyi.SSR1 with primer pair A.argyi.

[0029] Figure 8 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Xiangyang mugwort using primer pair A.argyi.SSR1.

[0030] Fig. 9 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying May Artemisia using primer pair A.argyi.SSR1.

[0031] Fig.10 Capillary electrophoresis results of PCR amplification products obtained by amplifying Korean mugwort using primer pair A.argyi.SSR1.

[0032] Fig.11 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Xi'an Ai using primer pair A.argyi.SSR1.

[0033] Fig.12 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Inner Mongolia Artemisia using primer pair A.argyi.SSR1.

[0034] Fig.13 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Artemisia liaotungensis using primer pair A.argyi.SSR1.

[0035] Fig.14 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Xinyang mugwort using primer pair A.argyi.SSR1.

[0036] Fig.15 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Tongchuan Artemisia using primer pair A.argyi.SSR1.

[0037] Fig.16 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Zheng Ai 2 using the primer pair A.argyi.SSR1.

[0038] Fig.17This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Luoyang mugwort using primer pair A.argyi.SSR1.

[0039] Fig.18 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Kaifeng Ai using primer pair A.argyi.SSR1.

[0040] Fig.19 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Hebei wild mugwort using primer pair A.argyi.SSR1.

[0041] Fig. 20 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Artemisia argyi using primer pair A.argyi.SSR1.

[0042] Fig.21 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Sanmenxia mugwort using primer pair A.argyi.SSR1.

[0043] Fig. 22 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Zhoukou Ai using primer pair A.argyi.SSR1.

[0044] Fig.23 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Artemisia argyi using primer pair A.argyi.SSR1.

[0045] Fig.24 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Zhumadian mugwort using primer pair A.argyi.SSR1.

[0046] Fig.25 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Tangyin Artemisia using primer pair A.argyi.SSR1.

[0047] Fig.26 Capillary electrophoresis results of PCR amplification products obtained by amplifying Xianggan Artemisia argyi using primer pair A.argyi.SSR1.

[0048] Fig. 27 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Suizhou Ai using primer pair A.argyi.SSR1.

[0049] Fig.28 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying A.argyi.SSR1 with the primer pair A.argyi.

[0050] Fig.29This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Artemisia argyi using primer pair A.argyi.SSR1.

[0051] Fig.30 Capillary electrophoresis results of PCR amplification products obtained by amplifying Xiaogan Ai using primer pair A.argyi.SSR1.

[0052] Fig.31 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying red-legged mugwort using primer pair A.argyi.SSR1.

[0053] Fig.32 This is the capillary electrophoresis result of the PCR amplification product obtained by using the primer pair A.argyi.SSR4 to amplify A.argyi.

[0054] Fig.33 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Xiangyang mugwort using primer pair A.argyi.SSR4.

[0055] Fig.34 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying May Artemisia using primer pair A.argyi.SSR4.

[0056] Fig.35 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Korean mugwort using primer pair A.argyi.SSR4.

[0057] Fig.36 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Xi'an Ai using primer pair A.argyi.SSR4.

[0058] Fig.37 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Inner Mongolia Artemisia using primer pair A.argyi.SSR4.

[0059] Fig.38 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Artemisia liaotungensis using primer pair A.argyi.SSR4.

[0060] Fig.39 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Xinyang mugwort using primer pair A.argyi.SSR4.

[0061] Fig.40 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Tongchuan Artemisia using primer pair A.argyi.SSR4.

[0062] Fig.41This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Zheng Ai 2 using the primer pair A.argyi.SSR4.

[0063] Fig.42 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Luoyang mugwort using primer pair A.argyi.SSR4.

[0064] Fig.43 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Kaifeng Ai using primer pair A.argyi.SSR4.

[0065] Fig.44 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Hebei wild mugwort using primer pair A.argyi.SSR4.

[0066] Fig.45 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Artemisia argyi using primer pair A.argyi.SSR4.

[0067] Fig.46 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Sanmenxia mugwort using primer pair A.argyi.SSR4.

[0068] Fig.47 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Zhoukou Ai using primer pair A.argyi.SSR4.

[0069] Fig.48 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Artemisia argyi using primer pair A.argyi.SSR4.

[0070] Fig.49 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Zhumadian mugwort using primer pair A.argyi.SSR4.

[0071] Fig.50 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Tangyin Ai using primer pair A.argyi.SSR4.

[0072] Fig.51 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Xianggan Artemisia argyi using primer pair A.argyi.SSR4.

[0073] Fig.52 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Suizhou Ai using primer pair A.argyi.SSR4.

[0074] Fig.53This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying A.argyi.SSR4 with the primer pair A.argyi.

[0075] Fig.54 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Artemisia argyi using primer pair A.argyi.SSR4.

[0076] Fig.55 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Xiaogan Ai using primer pair A.argyi.SSR4.

[0077] Fig.56 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying red-legged mugwort using primer pair A.argyi.SSR4.

[0078] Fig.57 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying A.argyi.SSR5 with primer pair A.argyi.

[0079] Fig.58 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Xiangyang mugwort using primer pair A.argyi.SSR5.

[0080] Fig.59 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying May Artemisia using primer pair A.argyi.SSR5.

[0081] Fig.60 Capillary electrophoresis results of PCR amplification products obtained by amplifying Korean mugwort using primer pair A.argyi.SSR5.

[0082] Fig.61 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Xi'an Ai using primer pair A.argyi.SSR5.

[0083] Fig.62 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Inner Mongolia Artemisia using primer pair A.argyi.SSR5.

[0084] Fig.63 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying A.argyi.SSR7 with primer pair A.argyi.

[0085] Fig.64 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Xiangyang mugwort using primer pair A.argyi.SSR7.

[0086] Fig.65This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying May Artemisia using primer pair A.argyi.SSR7.

[0087] Fig.66 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Korean mugwort using primer pair A.argyi.SSR7.

[0088] Fig.67 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Xi'an Ai using primer pair A.argyi.SSR7.

[0089] Fig.68 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Inner Mongolia Artemisia using primer pair A.argyi.SSR7.

[0090] Fig.69 This is the capillary electrophoresis result of the PCR amplification product obtained by using the primer pair A.argyi.SSR8 to amplify A.argyi.

[0091] Fig.70 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Xiangyang mugwort using primer pair A.argyi.SSR8.

[0092] Fig.71 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying May Artemisia using primer pair A.argyi.SSR8.

[0093] Fig.72 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Korean mugwort using primer pair A.argyi.SSR8.

[0094] Fig.73 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Xi'an Ai using primer pair A.argyi.SSR8.

[0095] Fig.74 This is the capillary electrophoresis result of the PCR amplification product obtained by amplifying Inner Mongolia Artemisia using primer pair A.argyi.SSR8. DETAILED DESCRIPTION

[0096] The present invention is further described in detail below in conjunction with specific embodiments. The examples given are only for illustrating the present invention, not for limiting the scope of the present invention. The examples provided below can be used as a guide for further improvements by those of ordinary skill in the art, and do not constitute a limitation of the present invention in any way. The experimental methods in the following examples, unless otherwise specified, are conventional methods, carried out according to the techniques or conditions described in the literature in this area or according to the product instructions. The materials, reagents, etc. used in the following examples, unless otherwise specified, can all be obtained from commercial sources. The quantitative tests in the following examples were all repeated three times, and the results were averaged.

[0097] QlAamp DNA Mini Kit is a product of QIAGEN, with a product number of 51306. Qubit DNA Assay Kit is a product of Invitrogen, with a product number of Q32854. 2×Precise PCRMix is ​​a product of Sino-Science, with a product number of XS22001. Trans 2K Plus DNA Marker is a product of Quanshijin, with a product number of E065.

[0098] The names of 25 mugwort varieties in the following examples and the literature recording their names are shown in Table 1.

[0099] Table 1. Names and origins of 25 mugwort varieties

[0100]

[0101]

[0102] Example, acquisition of primer combinations and their application in identifying 25 mugwort varieties

[0103] 1. Use QIAamp DNA Mini Kit to extract genomic DNA of the samples to be tested, and then use QubitDNA Assay Kit for DNA quantification. The samples to be tested are leaves of 25 varieties of mugwort, including Wan mugwort leaves, Xiangyang mugwort leaves, May mugwort leaves, Korean mugwort leaves, Xi'an mugwort leaves, Inner Mongolia mugwort leaves, Liaodong mugwort leaves, Xinyang mugwort leaves, Tongchuan mugwort leaves, Zheng mugwort leaves No. 2, Luoyang mugwort leaves, Kaifeng mugwort leaves, Hebei wild mugwort leaves, Pingdingshan mugwort leaves, Sanmenxia mugwort leaves, Zhoukou mugwort leaves, Da mugwort leaves, Zhumadian mugwort leaves, Tangyin mugwort leaves, Xianggan mugwort leaves, Suizhou mugwort leaves, Zhongnan mugwort leaves, Sichuan mugwort leaves, Xiaogan mugwort leaves and red-footed mugwort leaves.

[0104] 2. After completing step 1, the genomic DNA of the sample to be tested was tested on a 1% agarose gel. The loading amount was 100 ng.

[0105] This step is performed to check DNA integrity.

[0106] Some test results can be found in Figure 1 (The DNA Marker was Trans 2K Plus DNA Marker.) The results showed that the genomic DNA of the 25 mugwort varieties was intact.

[0107] 3. After completing step 2, use the genomic DNA of the sample to be tested as a template and use 30 primer pairs for PCR amplification to obtain PCR amplification products. The 30 primer pairs in this step are preliminarily screened by the inventor of this application based on the transcriptome sequencing data of existing mugwort varieties (including the above-mentioned 25 mugwort varieties). The reaction system is 25μL, consisting of 2μL of genomic DNA of the sample to be tested, 1μL of upstream primer F aqueous solution, 1μL of downstream primer R aqueous solution, 12.5μL 2×Precise PCR Mix and RNaseFree dH2O. The reaction procedure is: 94℃3min; 94℃30s, 50-60℃30s, 72℃30s, 35-40 cycles; 72℃5min; 4℃∞.

[0108] 4. The PCR amplification products obtained in step 3 were respectively detected by 2% agarose gel. The loading volume was 5 μL.

[0109] The purpose of this step is to observe the polymorphism of the primer pair.

[0110] The results of partial agarose gel electrophoresis of PCR amplification products obtained by using primer pair 1, primer pair 4, primer pair 5, primer pair 7 and primer pair 8 are shown in order. Figure 2-Figure 6 (The DNA Marker was Trans 2K Plus DNA Marker.) The results showed that primer pair 1, primer pair 4, primer pair 5, primer pair 7 and primer pair 8 all had good polymorphism.

[0111] The upstream primers in primer pair 1, primer pair 4, primer pair 5, primer pair 7 and primer pair 8 are synthesized into fluorescent primers (achieved by adding fluorescent groups, such as ROX, TAMRA, HEX, FAM, TAMRA), which are respectively composed of primer pair A.argyi.SSR1, primer pair A.argyi.SSR4, primer pair A.argyi.SSR5, primer pair A.argyi.SSR7 and primer pair A.argyi.SSR8.

[0112] The primer names, primer sequences and fluorescent colors of primer pair A.argyi.SSR1, primer pair A.argyi.SSR4, primer pair A.argyi.SSR5, primer pair A.argyi.SSR7 and primer pair A.argyi.SSR8 are shown in Table 2.

[0113] Table 2

[0114]

[0115] 5. After completing step 4, the genomic DNA of the sample to be tested is used as a template, and primer pair A.argyi.SSR1, primer pair A.argyi.SSR4, primer pair A.argyi.SSR5, primer pair A.argyi.SSR7 and primer pair A.argyi.SSR8 are used for PCR amplification to obtain PCR amplification products. The reaction system is 25 μL, consisting of 2 μL genomic DNA of the sample to be tested, 1 μL fluorescent primer F aqueous solution, 1 μL downstream primer R aqueous solution, 12.5 μL 2×Precise PCRMix and RNase Free dH2O. The reaction program is: 94℃3min; 94℃30s, 50-60℃30s, 72℃30s, 35-40 cycles; 72℃5min; 4℃∞.

[0116] 6. Perform capillary electrophoresis on the PCR amplification products obtained in step 5 respectively.

[0117] The primer pair A.argyi.SSR1 was used to amplify 25 varieties of mugwort. The capillary electrophoresis results of the PCR amplification products were shown in the following order: Figure 7-Figure 31 The primer pair A.argyi.SSR4 was used to amplify 25 varieties of mugwort. The capillary electrophoresis results of the PCR amplification products were shown in the following order: Figure 32-Figure 56 The primer pair A.argyi.SSR5 was used to amplify 25 varieties of mugwort. The capillary electrophoresis results of some of the PCR amplification products were shown in Figure 57-Figure 62 The primer pair A.argyi.SSR7 was used to amplify 25 varieties of mugwort. The capillary electrophoresis results of some of the PCR amplification products are shown in Figure 63-Figure 68 The primer pair A.argyi.SSR8 was used to amplify 25 varieties of mugwort. The capillary electrophoresis results of some of the PCR amplification products are shown in Figure 69-Figure 74 .

[0118] The above experiment was repeated three times, and the banding patterns of PCR amplification products of 25 mugwort varieties were counted to obtain fingerprints.

[0119] The banding patterns of the PCR amplification products of 25 mugwort varieties are shown in Tables 3, 4 and 5. It can be seen that the primer combination consisting of primer pair A.argyi.SSR1, primer pair A.argyi.SSR4, primer pair A.argyi.SSR5, primer pair A.argyi.SSR7 and primer pair A.argyi.SSR8 can identify 25 mugwort varieties.

[0120] Table 3

[0121]

[0122] Table 4

[0123]

[0124]

[0125] Table 5

[0126]

[0127] The above results show that the method provided by the present invention can identify whether the mugwort to be tested is Wan mugwort, Xiangyang mugwort, May mugwort, Korean mugwort, Xi'an mugwort, Inner Mongolia mugwort, Liaodong mugwort, Xinyang mugwort, Tongchuan mugwort, Zheng mugwort No. 2, Luoyang mugwort, Kaifeng mugwort, Hebei wild mugwort, Pingdingshan mugwort, Sanmenxia mugwort, Zhoukou mugwort, big mugwort, Zhumadian mugwort, Tangyin mugwort, Xiang-Gan mugwort, Suizhou mugwort, Central and South mugwort, Sichuan mugwort, Xiaogan mugwort and red-footed mugwort, thereby verifying the authenticity of 25 mugwort varieties.

[0128] The present invention has been described in detail above. For those skilled in the art, without departing from the purpose and scope of the present invention, and without the need to carry out unnecessary experimental conditions, the present invention can be implemented in a wide range under equivalent parameters, concentrations and conditions. Although the present invention provides specific embodiments, it should be understood that the present invention can be further improved. In a word, according to the principles of the present invention, the application is intended to include any changes, uses or improvements to the present invention, including departure from the disclosed scope in the application, and changes made with conventional techniques known in the art.

Claims

1. A primer combination, comprising primer pair A.argyi.SSR1, primer pair A.argyi.SSR4, primer pair A.argyi.SSR5, primer pair A.argyi.SSR7 and primer pair A.argyi.SSR8; The primer pair A.argyi.SSR1 consists of a primer A.argyi.SSR1-F having a nucleotide sequence as shown in SEQ ID NO:1 and a primer A.argyi.SSR1-R having a nucleotide sequence as shown in SEQ ID NO:2; The primer pair A.argyi.SSR4 consists of a primer A.argyi.SSR4-F having a nucleotide sequence as shown in SEQ ID NO:3 and a primer A.argyi.SSR4-R having a nucleotide sequence as shown in SEQ ID NO:4; The primer pair A.argyi.SSR5 consists of a primer A.argyi.SSR5-F having a nucleotide sequence as shown in SEQ ID NO:5 and a primer A.argyi.SSR5-R having a nucleotide sequence as shown in SEQ ID NO:6; The primer pair A.argyi.SSR7 consists of a primer A.argyi.SSR7-F having a nucleotide sequence as shown in SEQ ID NO:7 and a primer A.argyi.SSR7-R having a nucleotide sequence as shown in SEQ ID NO:8; The primer pair A.argyi.SSR8 consists of a primer A.argyi.SSR8-F having a nucleotide sequence as shown in SEQ ID NO:9 and a primer A.argyi.SSR8-R having a nucleotide sequence as shown in SEQ ID NO:

10.

2. The primer combination according to claim 1, characterized in that: The 5' ends of primer A.argyi.SSR1-F, primer A.argyi.SSR4-F, primer A.argyi.SSR5-F, primer A.argyi.SSR7-F and primer A.argyi.SSR8-F were modified with different fluorescent groups respectively.

3. The primer combination according to claim 1 or 2, characterized in that: The primer combination consists of primer pair A.argyi.SSR1, primer pair A.argyi.SSR4, primer pair A.argyi.SSR5, primer pair A.argyi.SSR7 and primer pair A.argyi.SSR8.

4. Use of the primer combination described in any one of claims 1 to 3 in identifying Wan mugwort, Xiangyang mugwort, May mugwort, Korean mugwort, Xi'an mugwort, Inner Mongolia mugwort, Liaodong mugwort, Xinyang mugwort, Tongchuan mugwort, Zheng mugwort No. 2, Luoyang mugwort, Kaifeng mugwort, Hebei wild mugwort, Pingdingshan mugwort, Sanmenxia mugwort, Zhoukou mugwort, big mugwort, Zhumadian mugwort, Tangyin mugwort, Hunan and Jiangxi mugwort, Suizhou mugwort, Central and South mugwort, Sichuan mugwort, Xiaogan mugwort and red-footed mugwort.

5. A method for identifying whether the mugwort to be tested is or is a candidate for being Wan mugwort, Xiangyang mugwort, May mugwort, Korean mugwort, Xi'an mugwort, Inner Mongolia mugwort, Liaodong mugwort, Xinyang mugwort, Tongchuan mugwort, Zheng mugwort No. 2, Luoyang mugwort, Kaifeng mugwort, Hebei wild mugwort, Pingdingshan mugwort, Sanmenxia mugwort, Zhoukou mugwort, big mugwort, Zhumadian mugwort, Tangyin mugwort, Xianggan mugwort, Suizhou mugwort, Central and Southern mugwort, Sichuan mugwort, Xiaogan mugwort and red-footed mugwort, comprising the following steps: using the genomic DNA of the mugwort to be tested as a template, respectively using the primer pairs A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 described in claims 1 to 3 to perform PCR amplification to obtain PCR amplification products; then performing the following judgment: If the primer pair A.argyi.SSR1 is used to amplify and obtain amplification products of 150bp, 161bp, 166bp and 170bp, the primer pair A.argyi.SSR4 is used to amplify and obtain amplification products of 139bp, 154bp and 157bp, the primer pair A.argyi.SSR5 is used to amplify and obtain amplification products of 138bp and 142bp, the primer pair A.argyi.SSR7 is used to amplify and obtain amplification products of 128bp and 137bp, and the primer pair A.argyi.SSR8 is used to amplify and obtain amplification products of 128bp, then the mugwort to be tested is or the candidate is Wan mugwort; If the primer pair A.argyi.SSR1 is used to amplify and obtain amplification products of 161bp and 170bp, the primer pair A.argyi.SSR4 is used to amplify and obtain amplification products of 139bp, 148bp and 157bp, the primer pair A.argyi.SSR5 is used to amplify and obtain amplification products of 133bp and 136bp, the primer pair A.argyi.SSR7 is used to amplify and obtain amplification products of 128bp and 137bp, and the primer pair A.argyi.SSR8 is used to amplify and obtain amplification products of 128bp, then the mugwort to be tested is or the candidate is Xiangyang mugwort; If the primer pair A.argyi.SSR1 is used to amplify and obtain amplification products of 150bp, 154bp and 161bp, the primer pair A.argyi.SSR4 is used to amplify and obtain amplification products of 139bp, 145bp and 154bp, the primer pair A.argyi.SSR5 is used to amplify and obtain amplification products of 133bp and 136bp, the primer pair A.argyi.SSR7 is used to amplify and obtain amplification products of 119bp and 137bp, and the primer pair A.argyi.SSR8 is used to amplify and obtain amplification products of 128bp, then the mugwort to be tested is or the candidate is Artemisia may; If the primer pair A.argyi.SSR1 is used to amplify and obtain amplification products of 161bp, 166bp and 170bp, the primer pair A.argyi.SSR4 is used to amplify and obtain amplification products of 139bp and 145bp, the primer pair A.argyi.SSR5 is used to amplify and obtain amplification products of 140bp and 148bp, the primer pair A.argyi.SSR7 is used to amplify and obtain amplification products of 128bp and 137bp, and the primer pair A.argyi.SSR8 is used to amplify and obtain amplification products of 128bp, then the mugwort to be tested is or the candidate is Korean mugwort; If the primer pair A.argyi.SSR1 is used to amplify to obtain an amplification product of 170 bp, the primer pair A.argyi.SSR4 is used to amplify to obtain an amplification product of 157 bp, the primer pair A.argyi.SSR5 is used to amplify to obtain an amplification product of 140 bp, the primer pair A.argyi.SSR7 is used to amplify to obtain an amplification product of 137 bp, and the primer pair A.argyi.SSR8 is used to amplify to obtain an amplification product of 128 bp, then the mugwort to be tested is or the candidate is Xi'an mugwort; If the primer pair A.argyi.SSR1 is used to amplify and obtain amplification products of 161bp and 170bp, the primer pair A.argyi.SSR4 is used to amplify and obtain amplification products of 139bp and 154bp, the primer pair A.argyi.SSR5 is used to amplify and obtain amplification products of 136bp and 140bp, the primer pair A.argyi.SSR7 is used to amplify and obtain amplification products of 128bp, and the primer pair A.argyi.SSR8 is used to amplify and obtain amplification products of 128bp, then the mugwort to be tested is or the candidate is Inner Mongolia mugwort; If the primer pair A.argyi.SSR1 is used to amplify and obtain amplification products of 161bp and 170bp, the primer pair A.argyi.SSR4 is used to amplify and obtain amplification products of 139bp, 145bp, 148bp and 154bp, the primer pair A.argyi.SSR5 is used to amplify and obtain amplification products of 136bp, 140bp and 142bp, the primer pair A.argyi.SSR7 is used to amplify and obtain amplification products of 128bp, 137bp and 154bp, and the primer pair A.argyi.SSR8 is used to amplify and obtain amplification product of 128bp, then the mugwort to be tested is or the candidate is Artemisia liaotungensis; If the primer pair A.argyi.SSR1 is used to amplify and obtain amplification products of 161bp and 170bp, the primer pair A.argyi.SSR4 is used to amplify and obtain amplification products of 139bp and 154bp, the primer pair A.argyi.SSR5 is used to amplify and obtain amplification products of 140bp and 142bp, the primer pair A.argyi.SSR7 is used to amplify and obtain amplification products of 119bp, 137bp and 140bp, and the primer pair A.argyi.SSR8 is used to amplify and obtain amplification products of 128bp, then the mugwort to be tested is or the candidate is Xinyang mugwort; If the primer pair A.argyi.SSR1 is used to amplify and obtain amplification products of 161bp, 170bp and 175bp, the primer pair A.argyi.SSR4 is used to amplify and obtain amplification products of 139bp and 148bp, the primer pair A.argyi.SSR5 is used to amplify and obtain amplification products of 140bp and 142bp, the primer pair A.argyi.SSR7 is used to amplify and obtain amplification products of 137bp, and the primer pair A.argyi.SSR8 is used to amplify and obtain amplification products of 128bp and 132bp, then the mugwort to be tested is or the candidate is Tongchuan mugwort; If the primer pair A.argyi.SSR1 is used to amplify and obtain amplification products of 161bp and 170bp, the primer pair A.argyi.SSR4 is used to amplify and obtain amplification products of 139bp, 148bp, 154bp and 157bp, the primer pair A.argyi.SSR5 is used to amplify and obtain amplification products of 133bp and 136bp, the primer pair A.argyi.SSR7 is used to amplify and obtain amplification products of 137bp and 154bp, and the primer pair A.argyi.SSR8 is used to amplify and obtain amplification products of 128bp, then the mugwort to be tested is or the candidate is Zheng Ai No. 2; If the primer pair A.argyi.SSR1 is used to amplify and obtain amplification products of 161bp, 166bp and 170bp, the primer pair A.argyi.SSR4 is used to amplify and obtain amplification products of 139bp, 145bp, 148bp and 154bp, the primer pair A.argyi.SSR5 is used to amplify and obtain amplification products of 133bp, 136bp and 140bp, the primer pair A.argyi.SSR7 is used to amplify and obtain amplification products of 119bp, 137bp, 140bp and 154bp, and the primer pair A.argyi.SSR8 is used to amplify and obtain amplification products of 128bp, then the mugwort to be tested is or the candidate is Luoyang mugwort; If the primer pair A.argyi.SSR1 is used to amplify and obtain amplification products of 150bp, 161bp and 170bp, the primer pair A.argyi.SSR4 is used to amplify and obtain amplification products of 145bp, 148bp and 154bp, the primer pair A.argyi.SSR5 is used to amplify and obtain amplification products of 136bp, 140bp and 148bp, the primer pair A.argyi.SSR7 is used to amplify and obtain amplification products of 128bp, and the primer pair A.argyi.SSR8 is used to amplify and obtain amplification products of 128bp, then the mugwort to be tested is or the candidate is Kaifeng mugwort; If the primer pair A.argyi.SSR1 is used to amplify and obtain amplification products of 161bp and 170bp, the primer pair A.argyi.SSR4 is used to amplify and obtain amplification products of 139bp, 148bp and 154bp, the primer pair A.argyi.SSR5 is used to amplify and obtain amplification products of 133bp, 136bp and 140bp, the primer pair A.argyi.SSR7 is used to amplify and obtain amplification products of 128bp, and the primer pair A.argyi.SSR8 is used to amplify and obtain amplification products of 128bp, then the mugwort to be tested is or the candidate is Hebei wild mugwort; If the primer pair A.argyi.SSR1 is used to amplify and obtain amplification products of 161bp, 170bp and 175bp, the primer pair A.argyi.SSR4 is used to amplify and obtain amplification products of 139bp, the primer pair A.argyi.SSR5 is used to amplify and obtain amplification products of 133bp, 136bp and 140bp, the primer pair A.argyi.SSR7 is used to amplify and obtain amplification products of 119bp, 128bp and 137bp, and the primer pair A.argyi.SSR8 is used to amplify and obtain amplification products of 128bp, then the mugwort to be tested is or the candidate is Artemisia sylvestris; If the primer pair A.argyi.SSR1 is used to amplify and obtain amplification products of 161bp and 170bp, the primer pair A.argyi.SSR4 is used to amplify and obtain amplification products of 145bp, 148bp and 154bp, the primer pair A.argyi.SSR5 is used to amplify and obtain amplification products of 133bp, 140bp and 142bp, the primer pair A.argyi.SSR7 is used to amplify and obtain amplification products of 128bp, and the primer pair A.argyi.SSR8 is used to amplify and obtain amplification products of 128bp, then the mugwort to be tested is or the candidate is Sanmenxia mugwort; If the primer pair A.argyi.SSR1 is used to amplify and obtain an amplification product of 166 bp, the primer pair A.argyi.SSR4 is used to amplify and obtain an amplification product of 142 bp and 145 bp, the primer pair A.argyi.SSR5 is used to amplify and obtain an amplification product of 136 bp and 140 bp, the primer pair A.argyi.SSR7 is used to amplify and obtain an amplification product of 119 bp and 140 bp, and the primer pair A.argyi.SSR8 is used to amplify and obtain an amplification product of 110 bp and 128 bp, then the mugwort to be tested is or the candidate is Zhoukou mugwort; If the primer pair A.argyi.SSR1 is used to amplify an amplification product of 170 bp, the primer pair A.argyi.SSR4 is used to amplify an amplification product of 142 bp and 145 bp, the primer pair A.argyi.SSR5 is used to amplify an amplification product of 136 bp and 152 bp, the primer pair A.argyi.SSR7 is used to amplify an amplification product of 119 bp and 137 bp, and the primer pair A.argyi.SSR8 is used to amplify an amplification product of 110 bp, then the mugwort to be tested is or the candidate is Artemisia argyi; If the primer pair A.argyi.SSR1 is used to amplify and obtain amplification products of 150bp, 166bp and 170bp, the primer pair A.argyi.SSR4 is used to amplify and obtain amplification products of 139bp and 148bp, the primer pair A.argyi.SSR5 is used to amplify and obtain amplification products of 124bp, 133bp and 140bp, the primer pair A.argyi.SSR7 is used to amplify and obtain amplification products of 137bp, and the primer pair A.argyi.SSR8 is used to amplify and obtain amplification products of 128bp, then the mugwort to be tested is or the candidate is Zhumadian mugwort; If the primer pair A.argyi.SSR1 is used to amplify and obtain amplification products of 161bp, 166bp and 170bp, the primer pair A.argyi.SSR4 is used to amplify and obtain amplification products of 139bp and 145bp, the primer pair A.argyi.SSR5 is used to amplify and obtain amplification products of 124bp, 133bp, 136bp, 140bp and 142bp, the primer pair A.argyi.SSR7 is used to amplify and obtain amplification product of 137bp, and the primer pair A.argyi.SSR8 is used to amplify and obtain amplification product of 128bp, then the mugwort to be tested is or the candidate is Tangyin mugwort; If the primer pair A.argyi.SSR1 is used to amplify an amplification product of 170 bp, the primer pair A.argyi.SSR4 is used to amplify an amplification product of 139 bp, 145 bp and 154 bp, the primer pair A.argyi.SSR5 is used to amplify an amplification product of 133 bp, 136 bp and 140 bp, the primer pair A.argyi.SSR7 is used to amplify an amplification product of 137 bp, and the primer pair A.argyi.SSR8 is used to amplify an amplification product of 128 bp, then the mugwort to be tested is or the candidate is Xianggan mugwort; If the primer pair A.argyi.SSR1 is used to amplify and obtain amplification products of 161bp, 166bp and 170bp, the primer pair A.argyi.SSR4 is used to amplify and obtain amplification products of 139bp, 145bp and 148bp, the primer pair A.argyi.SSR5 is used to amplify and obtain amplification products of 133bp, 136bp and 142bp, the primer pair A.argyi.SSR7 is used to amplify and obtain amplification products of 119bp, 128bp, 137bp and 154bp, and the primer pair A.argyi.SSR8 is used to amplify and obtain amplification products of 128bp, then the mugwort to be tested is or the candidate is Suizhou mugwort; If the primer pair A.argyi.SSR1 is used to amplify and obtain amplification products of 161bp, 166bp and 170bp, the primer pair A.argyi.SSR4 is used to amplify and obtain amplification products of 139bp and 145bp, the primer pair A.argyi.SSR5 is used to amplify and obtain amplification products of 133bp and 136bp, the primer pair A.argyi.SSR7 is used to amplify and obtain amplification products of 119bp, 137bp and 154bp, and the primer pair A.argyi.SSR8 is used to amplify and obtain amplification products of 120bp and 128bp, then the mugwort to be tested is or the candidate is mugwort in central and southern China; If the primer pair A.argyi.SSR1 is used to amplify and obtain amplification products of 154bp, 161bp, 166bp and 170bp, the primer pair A.argyi.SSR4 is used to amplify and obtain amplification products of 139bp and 145bp, the primer pair A.argyi.SSR5 is used to amplify and obtain amplification products of 133bp, 136bp and 142bp, the primer pair A.argyi.SSR7 is used to amplify and obtain amplification products of 119bp and 140bp, and the primer pair A.argyi.SSR8 is used to amplify and obtain amplification products of 128bp, then the mugwort to be tested is or the candidate is Artemisia argyi; If the primer pair A.argyi.SSR1 is used to amplify to obtain amplification products of 161bp and 166bp, the primer pair A.argyi.SSR4 is used to amplify to obtain amplification products of 142bp and 154bp, the primer pair A.argyi.SSR5 is used to amplify to obtain amplification products of 133bp and 140bp, the primer pair A.argyi.SSR7 is used to amplify to obtain amplification products of 137bp and 140bp, and the primer pair A.argyi.SSR8 is used to amplify to obtain amplification products of 128bp, then the mugwort to be tested is or the candidate is Xiaogan mugwort; If the primer pair A.argyi.SSR1 can obtain amplification products of 161bp and 170bp, the primer pair A.argyi.SSR4 can obtain amplification products of 139bp and 145bp, the primer pair A.argyi.SSR5 can obtain amplification products of 136bp, 138bp and 142bp, the primer pair A.argyi.SSR7 can obtain an amplification product of 119bp, and the primer pair A.argyi.SSR8 can obtain an amplification product of 128bp, then the mugwort to be tested is or is a candidate for red-footed mugwort.

6. A method for identifying whether the mugwort to be tested is or is a candidate for being Wan mugwort, Xiangyang mugwort, May mugwort, Korean mugwort, Xi'an mugwort, Inner Mongolia mugwort, Liaodong mugwort, Xinyang mugwort, Tongchuan mugwort, Zheng mugwort No. 2, Luoyang mugwort, Kaifeng mugwort, Hebei wild mugwort, Pingdingshan mugwort, Sanmenxia mugwort, Zhoukou mugwort, big mugwort, Zhumadian mugwort, Tangyin mugwort, Xianggan mugwort, Suizhou mugwort, Central and South mugwort, Sichuan mugwort, Xiaogan mugwort and red-footed mugwort, comprising the following steps: (1) Using the genomic DNA of the mugwort to be tested as a template, PCR amplification is performed using the primer pairs A.argyi.SSR1, A.argyi.SSR4, A.argyi.SSR5, A.argyi.SSR7 and A.argyi.SSR8 described in claims 1 to 3 to obtain PCR amplification products; (2) using the genomic DNA of Wan mugwort, Xiangyang mugwort, May mugwort, Korean mugwort, Xi'an mugwort, Inner Mongolia mugwort, Liaodong mugwort, Xinyang mugwort, Tongchuan mugwort, Zheng mugwort No. 2, Luoyang mugwort, Kaifeng mugwort, Hebei wild mugwort, Pingdingshan mugwort, Sanmenxia mugwort, Zhoukou mugwort, big mugwort, Zhumadian mugwort, Tangyin mugwort, Xianggan mugwort, Suizhou mugwort, Central and South mugwort, Sichuan mugwort, Xiaogan mugwort and red-footed mugwort as templates, respectively, using the primer pairs A.argyi.SSR1, A.argyi.SSR4, A.argyi.SSR5, A.argyi.SSR7 and A.argyi.SSR8 described in claims 1 to 3 to perform PCR amplification to obtain PCR amplification products; (3) Make the following judgment: If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Wan mugwort, then the mugwort to be tested is or the candidate is Wan mugwort; If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Xiangyang mugwort, then the mugwort to be tested is or the candidate is Xiangyang mugwort; If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Artemisia mays, then the mugwort to be tested is or the candidate is Artemisia mays; If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Korean mugwort, then the mugwort to be tested is or the candidate is Korean mugwort; If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Xi'an mugwort, then the mugwort to be tested is or the candidate is Xi'an mugwort; If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Inner Mongolia mugwort, then the mugwort to be tested is or the candidate is Inner Mongolia mugwort; If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Artemisia liaotungensis, then the mugwort to be tested is or is a candidate for Artemisia liaodongensis; If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Xinyang mugwort, then the mugwort to be tested is or the candidate is Xinyang mugwort; If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Tongchuan mugwort, then the mugwort to be tested is or the candidate is Tongchuan mugwort; If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Zheng Ai No. 2, then the mugwort to be tested is or the candidate is Zheng Ai No. 2; If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Luoyang mugwort, then the mugwort to be tested is or the candidate is Luoyang mugwort; If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Kaifeng mugwort, then the mugwort to be tested is or the candidate is Kaifeng mugwort; If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Hebei wild mugwort, then the mugwort to be tested is or the candidate is Hebei wild mugwort; If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Pinus tabebuia respectively, then the mugwort to be tested is or the candidate is Pinus tabebuia; If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Sanmenxia mugwort, then the mugwort to be tested is or the candidate is Sanmenxia mugwort; If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Zhoukou mugwort, then the mugwort to be tested is or the candidate is Zhoukou mugwort; If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the big mugwort, then the mugwort to be tested is or the candidate is the big mugwort; If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Zhumadian mugwort, then the mugwort to be tested is or the candidate is Zhumadian mugwort; If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Tangyin mugwort, then the mugwort to be tested is or the candidate is Tangyin mugwort; If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Xianggan mugwort, then the mugwort to be tested is or the candidate is Xianggan mugwort; If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Suizhou mugwort, then the mugwort to be tested is or the candidate is Suizhou mugwort; If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the central and southern mugwort, then the mugwort to be tested is or the candidate is central and southern mugwort; If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Sichuan mugwort, then the mugwort to be tested is or the candidate is Sichuan mugwort; If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are all consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for Xiaogan mugwort, then the mugwort to be tested is or the candidate is Xiaogan mugwort; If the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for the mugwort to be tested are consistent in size with the PCR amplification products obtained by using the primer pair A.argyi.SSR1, the primer pair A.argyi.SSR4, the primer pair A.argyi.SSR5, the primer pair A.argyi.SSR7 and the primer pair A.argyi.SSR8 for red-legged mugwort, then the mugwort to be tested is or is a candidate for red-legged mugwort.

7. The method according to claim 5 or 6, characterized in that: The PCR amplification product was detected by electrophoresis.

8. The method according to claim 7, characterized in that: The electrophoresis is capillary electrophoresis.

9. A kit comprising the primer combination according to any one of claims 1 to 3.

10. Use of the kit according to claim 9 in identifying Wan mugwort, Xiangyang mugwort, May mugwort, Korean mugwort, Xi'an mugwort, Inner Mongolia mugwort, Liaodong mugwort, Xinyang mugwort, Tongchuan mugwort, Zheng mugwort No. 2, Luoyang mugwort, Kaifeng mugwort, Hebei wild mugwort, Pingdingshan mugwort, Sanmenxia mugwort, Zhoukou mugwort, big mugwort, Zhumadian mugwort, Tangyin mugwort, Hunan and Jiangxi mugwort, Suizhou mugwort, Central and South mugwort, Sichuan mugwort, Xiaogan mugwort and red-footed mugwort.

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