Co-separation molecular marker of rice hybrid male sterility site HMS10 and application of co-separation molecular marker
By finely positioning the heterozygous male sterile site of rice and developing the co-isolated molecular marker IDHMS10, the problems of high seed production risks and single sterile resources in the prior art are solved, and the selection and breeding of heterozygous male sterile lines with stable fertility and free coordination groups are achieved, and the safety of seed production is improved.
CN119710074AActive Publication Date: 2025-03-28RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI +1
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Patent Information
- Application Number
- CN202510154805.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-12
- Publication Date
- 2025-03-28
- Estimated Expiration
- 2045-02-12
AI Technical Summary
Technical Problem
The existing two-line hybrid rice technology faces problems such as high risk of seed production, narrow breeding areas of sterile lines, and relatively single types of practical sterile resources. Especially in the complex and changing global climate, seed production safety is threatened.
Method used
The heterozygous male sterility site of rice was controlled by fine positioning, and the co-isolated molecular marker IDHMS10 was developed for molecular breeding and purity identification of heterozygous male sterile lines.
Benefits of technology
The breeding of heterozygous male sterile lines with no environmental impact and relatively free combination is achieved, and the seed production safety and breeding application prospects of hybrid rice are improved.
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Abstract
The invention belongs to the technical field of crop genetic breeding, and particularly relates to a rice hybrid male sterility site HMS10 co-segregation molecular marker and application thereof. The method comprises the following steps: hybridizing and backcrossing an anti-thermo-sensitive type genic male sterile line Yannong S serving as a female parent with a normal fertile material Yannong HB (serving as a recurrent male parent), finely positioning to a site HMS10 for controlling hybrid male sterility of rice in a physical interval of-60kb of a chromosome 10 of the rice by utilizing a high-generation backcross large population, and developing a co-separation molecular marker IDHMS10. The molecular marker IDHMS10 is utilized, through a molecular breeding strategy, a positive temperature-sensitive male genic male sterile line Quan 211S with excellent comprehensive agronomic traits is transformed into a practical rice hybrid male sterile line Quan 211HS with fertility not affected by light and temperature, and the IDHMS10 is used for purity identification of the hybrid male sterile line, so that the molecular marker IDHMS10 has great significance in solving the seed production risk of two-line hybrid rice and improving the yield of the hybrid male sterile line. And the method has important significance in enriching practical sterile resource types and the like.
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