Preparation and application of novel diagnostic kit for evaluating regeneration capacity function of mitochondrial network

By preparing solid-phase carriers and specific antibodies in 96-well microplate, the concentrations of DRPI, MFN2, OPA1 and TIM23 were detected by the dual-antibody sandwich method, the problem of difficulty in accurately evaluating mitochondrial network function in the prior art was solved, and the accurate evaluation of mitochondrial network regeneration function was achieved.

CN119716091APending Publication Date: 2025-03-28GUANGZHOU HONGWEI BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202510013683.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-01-06
Publication Date
2025-03-28

AI Technical Summary

Technical Problem

There is no diagnostic kit for evaluating mitochondrial network function in the prior art, especially the combination of four proteins, DRPI, MFN2, OPA1 and TIM23, which is difficult to accurately reflect the cell mitochondrial network regeneration ability.

Method used

The solid phase carrier was prepared in 96-well microplate, coated with specific antibodies, and the concentrations of DRPI, MFN2, OPA1 and TIM23 in the sample were detected by the diabodyne sandwich method. The concentration of each protein was calculated by standard curves, so as to judge the mitochondrial network regeneration function of the sample.

Benefits of technology

Accurate evaluation of the cell mitochondrial network regeneration function is achieved, and the equilibrium state of mitochondrial fusion and division can be judged, and then the good and bad mitochondrial function can be evaluated.

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Abstract

The invention discloses preparation and application of a novel diagnostic kit for a mitochondrial network regeneration function evaluation test, and in the invention, combined detection of related proteins DRP1, MFN2, OPA1 and TIM23 is carried out by utilizing four mitochondrial networks, so that the mitochondrial regeneration function state of sample cells is judged. The kit disclosed by the invention is used for detecting samples such as human blood, urine, cell cultures and tissues by utilizing an ELISA (Enzyme-Linked Immunosorbent Assay) method. Results show that the kit can accurately detect the content of four proteins so as to judge the function of sample mitochondria. The mitochondrial network regeneration capacity function evaluation kit based on a double-antibody sandwich method in an ELISA method can conveniently and accurately evaluate the mitochondrial network regeneration capacity in a human body specimen. The invention discloses preparation and application of the novel diagnostic kit for evaluating the regeneration function of the mitochondrial network. The diagnostic kit plays an auxiliary role in mitochondrial detection in scientific research and disease diagnosis and treatment. The method is applied to mitochondrial function evaluation in all diseases with abnormal cell mitochondrial functions.
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