Bladder cancer fine typing method and system based on aptamer difference analysis

Through the method based on the differential analysis of nucleic acid aptamers, sequencing data and differentially expressed aptamers are obtained and processed, which solves the problem of precise typing of bladder cancer, achieves more detailed and accurate typing, and improves the personalization of the treatment plan and the accuracy of diagnosis.

CN119724344AActive Publication Date: 2025-03-28ZHEJIANG UNIV OF TECH +1
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Patent Information

Application Number
CN202411769306.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-04
Publication Date
2025-03-28
Estimated Expiration
2044-12-04

AI Technical Summary

Technical Problem

The prior art is difficult to achieve precise typing of bladder cancer, and the nucleic acid aptamer of a single marker is not sufficient to cope with the high heterogeneity of bladder cancer.

Method used

By obtaining sequencing data, abnormal samples are removed and standardized, differential expression aptamers are selected, and the samples to be tested are then typed. Specific steps include high-throughput gene sequencing, standardization of nucleic acid aptamer data, and selection of differentially expressed aptamer.

Benefits of technology

The fine segmentation of bladder cancer cells has been achieved, the ability to identify tumor cells has been improved, and important support has been provided for the development of personalized treatment plans, reducing the possibility of misdiagnosis and missed diagnosis, and improving the accuracy of diagnosis.

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Abstract

The invention relates to a bladder cancer fine typing method and system based on aptamer difference analysis, and the method comprises the steps: incubating a sample containing bladder cancer cells through a probe package, and carrying out high-throughput gene sequencing on the incubated sample to obtain sequencing data; performing abnormal sample removal on the sequencing data to obtain aptamer data; performing standardization processing on the aptamer data to obtain standard data including a healthy sample group and a case sample group; selecting a differential expression aptamer from the standard data; and typing a to-be-detected sample through the differential expression aptamer. According to the present invention, the expression difference of the specific nucleic acid aptamer in the bladder cancer cell can be identified so as to achieve the fine typing.
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