Non-animal protein blends and compositions containing the same

Abstract

Non-animal protein blends comprising a pulse protein and one or more of a synthetic protein, a seed protein, and a cereal protein are described, wherein the PDCAAS of the blend is 1. Compositions comprising these blends and methods of using the same are also disclosed.

Description

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS

[0002] This application claims the benefit of priority to U.S. Provisional Application No. 63 / 341,773, filed on May 13, 2022, which is incorporated herein by reference in its entirety. Technical Field

[0003] The present disclosure relates to plant-based protein blends, nutritional compositions and supplements containing the blends, and methods of use thereof.

[0004] background

[0005] Vegetarian diets rely on plant sources such as fruits, vegetables, nuts, and whole grains rather than animal sources such as meat and dairy. While vegetarian diets are popular for a variety of reasons, protein intake in vegetarian diets may be lower than meat-based diets, resulting in the body not containing adequate amounts of the essential amino acids it needs. The nine essential amino acids that are often lacking in vegetarian diets are histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine.

[0006] To assess the quality of dietary protein, the United States Food and Drug Administration (US FDA) and the Food and Agriculture Organization / World Health Organization (FAO / WHO) adopted the Protein Digestibility Corrected Amino Acid Score (PDCAAS) in 1993 as a method based on both the body's amino acid requirements and its ability to digest protein. Since then, the PDCAAS score has been recognized as the preferred method for determining protein quality. Protein is assessed on a PDCAAS scale of 0 to 1, with 1 being the highest. The PDCAAS is calculated as follows:

[0007]

[0008] The essential amino acid profile of the reference protein was selected as the amino acid requirement pattern for children aged 2-5 years, as shown in Table 1.

[0009] Table 1: Essential amino acid profiles of reference proteins used for PDCAAS determination

[0010] Essential Amino Acids Protein mg / g Histidine 19 Isoleucine 28 Leucine 66 Lysine 58 Methionine + Cystine 25 Phenylalanine+ 63 Threonine 34 Tryptophan 11 Valine 35

[0011] Animal-based proteins such as casein, whey protein, and egg white protein score 1.00 on the PDCAAS scale, while plant-based foods and proteins typically score lower. For example, vegetables score between 0.7-0.89, fresh fruits score between 0.64-0.76, and legumes score between 0.7-0.89. One way to address the incomplete amino acid profile of plant-based proteins is to prepare protein blends that can serve as a complete and balanced amino acid source for the physiological needs of the human body. To calculate the PDCAAS for a protein blend, take a weighted average of the individual amino acids of each protein in the blend to determine the limiting amino acid, and take a weighted average of the digestibility percentage of each protein, and substitute these numbers into the above equation. Although the PDCAAS score of a protein blend can be higher than 1, standard practice is to truncate the score to 1.

[0012] Obtaining a non-animal protein blend with a PDCAAS of 1 can be a challenging task. Proteins obtained from different suppliers can have different amino acid profiles, the availability of protein can be different, and dietary preferences in certain regions can also be different. These factors and others make it difficult to find a suitable blend with a PDCAAS of 1. In addition, many vegetarian diets rely heavily on soy protein, which has a complete amino acid profile and high digestibility. But soy can cause allergies, the phytoestrogens it contains can affect the body's natural estrogen activity, and there are sustainability challenges in terms of land and fertilizer use. In addition, mixing different non-animal protein sources brings challenges related to taste, cost, and whether the blend can be used in shelf-stable beverage products. Therefore, what is needed is a blend of non-animal proteins that does not rely on soy protein as the main protein source, has a PDCAAS of 1, and has an ideal taste. The compositions and methods disclosed herein meet these and other needs.

[0013] Overview

[0014] Disclosed herein are compositions, compounds, methods for making and using such compounds and compositions. In one aspect, a non-animal protein blend comprising a soy protein and one or more of a synthetic protein, a seed protein, and a grain protein is disclosed, wherein the PDCAAS of the blend is 1. In one aspect, a non-animal protein blend comprising a soy protein, beta-lactoglobulin, and optionally a grain protein is disclosed, wherein the PDCAAS of the blend is 1. In another aspect, a non-animal protein blend comprising a soy protein, a seed protein, and optionally a grain protein is disclosed, wherein the PDCAAS of the blend is 1. In a further aspect, a non-animal protein blend comprising ovomucoid, a soy protein, a seed protein, and optionally a grain protein is disclosed, wherein the PDCAAS of the blend is 1. The disclosed blends do not contain animal protein, but may optionally contain one or more of fiber, prebiotics, probiotics, digestive enzymes, sweeteners, flavorings, colorants, vitamins, and minerals.

[0015] In some examples of the disclosed blends, the legume protein can be chickpea protein, pea protein, lentil protein, or a combination thereof. In other examples, the seed protein can be canola protein, sacha inchi protein, or a combination thereof. In other examples, the grain protein can be oat protein.

[0016] Also described herein are compositions and beverages containing the disclosed blends, as well as methods of making and using the blends and compositions.

[0017] Additional advantages will be set forth in part in the description that follows, and in part will be apparent from the description, or may be learned by practicing the aspects described below. The advantages described below will be realized and obtained by the elements and combinations particularly pointed out in the appended claims. It should be understood that both the foregoing general description and the following detailed description are exemplary and illustrative only and are not restrictive.

[0018] Detailed Description

[0019] The present invention may be understood more readily by reference to the following detailed description of the invention and the Examples included therein.

[0020] Before disclosing and describing the compounds, compositions and / or methods of the present invention, it should be understood that unless otherwise specified, it is not limited to a specific synthesis method, or unless otherwise specified, it is not limited to a specific reagent, and therefore can certainly vary. It should also be understood that the terminology used herein is only used for the purpose of describing specific aspects and is not intended to be limiting. Although any methods and materials similar or equivalent to the methods and materials described herein can be used in the practice or testing of the present invention, only exemplary methods and materials are now described.

[0021] General Definition

[0022] In this specification and the claims that follow, reference will be made to a number of terms that are defined as having the following meanings:

[0023] As used in the specification and in the claims, the term "comprising" may include aspects of "consisting of" and "consisting essentially of". Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the invention belongs. In this specification and the claims that follow, reference will be made to various terms defined herein.

[0024] The terms "for example" and "such as" and their grammatical equivalents, unless expressly stated otherwise, should be understood to be followed by the phrase "without limitation."

[0025] In this specification, numerical ranges are provided for certain quantities. It should be understood that these ranges include all values ​​and subranges therein. Therefore, the range "50 to 80" includes all possible values ​​therein (e.g., 50, 51, 52, 53, 54, 55, 56, etc.) and all possible ranges therein (e.g., 51-79, 52-78, 53-77, 54-76, 55-75, 60-70, etc.). In addition, all values ​​in a given range can be the endpoints of the range (e.g., the range 50-80 includes a range with endpoints, such as 55-80, 50-75, etc.).

[0026] Ranges can be expressed herein as from "about" a particular value and / or to "about" another particular value. When such a range is expressed, another aspect includes from a particular value and / or to another particular value. Similarly, when a value is expressed as an approximation, by using the antecedent "about", it will be understood that the particular value forms another aspect. It will be further understood that the endpoints of each range are important relative to the other endpoint and independently of the other endpoint. It should also be understood that multiple values ​​are disclosed herein, and each value is also disclosed herein as "about" that particular value in addition to the value itself. For example, if the value "10" is disclosed, "about 10" is also disclosed. In a specific example, "about" means within 5%, 4%, 3%, 2% or 1% of the value.

[0027] The term "a" or "an" refers to one or more of that entity; for example, "a protein" refers to one or more proteins. Thus, the terms "a", "one or more", and "at least one" are used interchangeably herein. Furthermore, reference to a "protein" by the indefinite article "a" or "an" does not exclude the possibility that more than one protein is present, unless the context clearly requires that one and only one protein is present.

[0028] References in the specification and in the concluding claims to parts by weight of a particular element or component in a composition indicate the weight relationship, expressed in parts by weight, between that element or component and any other element or component in the composition or article. Thus, in a mixture containing 2 parts by weight of component X and 5 parts by weight of component Y, X and Y are present in a weight ratio of 2:5, and are present in this ratio regardless of whether the mixture contains additional components.

[0029] Unless expressly stated to the contrary, the weight percent (wt. %) of a component is based on the total weight of the formulation or composition in which the component is included.

[0030] The term "amino acid" refers to the naturally occurring amino acids: alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine.

[0031] As used herein, the term "administering" refers to any method of providing a formulation to a subject. Such methods are well known to those skilled in the art. In preferred methods, administration refers to oral administration. Administration may be continuous or intermittent. In some instances, the formulation may be administered therapeutically; that is, administered to treat an existing disease or condition. In some instances, the formulation may be administered prophylactically; that is, administered to prevent the treatment of a disease or condition.

[0032] "Dosage form" includes any preparation or combination of preparations that provide a desired dosage. Therefore, a dosage form can include a single composition (such as a powdered drink mix or other ingestible preparation) or a combination of several different compositions (such as a powdered drink mix, snacks and supplements). The dosage form can "provide a daily dose" in a single unit dosage form (such as a tablet or a liquid drink dispersed with a powder mixture) or in multiple doses taken at different times of the day. Therefore, a dosage form including multiple sub-dosage forms can provide a total daily dose for administration at different times of the day (e.g., at breakfast and lunch) and in different forms (e.g., as a liquid drink and a chewable snack bar). A specific example of a dosage form is an artificial preparation including a drug carrier. Further distinctions can be made between exogenous preparations and food preparations, wherein exogenous preparations are added outside of consuming conventional foods.

[0033] As used herein, the term "effective amount" refers to an amount sufficient to achieve a desired result or to affect an undesirable condition. The specific effective amount for any particular subject will depend on a variety of factors, including the subject's age, weight, general health, sex, and diet. For example, one skilled in the art can start the dosage of the composition at a dosage level lower than that required to achieve the desired effect and gradually increase the dosage until the desired effect is achieved. If desired, the daily effective amount can be divided into multiple doses for administration. Therefore, a single dose composition can contain such an amount or its approximate multiple to constitute a daily dose.

[0034] "Optional" or "optionally" means that the subsequently described event or circumstance may or may not occur, and that the description includes instances where the event or circumstance occurs and instances where it does not.

[0035] As used herein, the term "substantially" means that the subsequently described event or circumstance completely occurs, or that the subsequently described event or circumstance generally, typically, or approximately occurs.

[0036] Furthermore, the term "substantially" may refer in some aspects to at least about 80%, at least about 85%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or about 100% of a stated characteristic, feature, component, composition, or other condition (substantially used to characterize or otherwise quantify a quantity), both instances in which the event or condition occurs and instances in which it does not occur.

[0037] Disclosed are the components for preparing the compositions disclosed herein and the compositions themselves used in the methods disclosed herein. These and other materials are disclosed herein, and it should be understood that when the combinations, subsets, groups, etc. of these materials are disclosed, although the specific references of each different individual and common combination and arrangement of these compounds may not be explicitly disclosed, each is explicitly covered and described herein. For example, if a specific compound is disclosed and discussed, and multiple modifications that can be made to multiple molecules including these compounds are discussed, each combination and arrangement of the compound and possible modifications are explicitly covered unless there is an explicit statement to the contrary. Therefore, if an example of a class of molecules A, B and C and a class of molecules D, E and F and a combination molecule AD is disclosed, each is individually and collectively covered even if each is not listed separately, meaning that the combinations AE, AF, BD, BE, BF, CD, CE and CF are considered to have been disclosed. Similarly, any subset or combination of these is also disclosed. Therefore, for example, the subgroups of AE, BF and CE are considered to have been disclosed. This concept applies to all aspects of the present application, including but not limited to steps in the method of making and using the compositions disclosed herein. Thus, if there are a variety of additional steps that can be performed it is understood that each of these additional steps can be performed with any specific embodiment or combination of embodiments of the methods disclosed herein.

[0038] As used herein, the term "individual" or "subject" refers to an animal, such as a mammal, for example, a human.

[0039] Reference will now be made in detail to certain aspects of the disclosed materials, compounds, compositions, articles, and methods, examples of which are illustrated in the accompanying Examples.

[0040] Protein Blends

[0041] Disclosed herein are blends of non-animal proteins having an overall PDCAAS of 1. The disclosed blends may also have a desirable taste alone or in a beverage or food composition. The protein blends disclosed herein contain more than one non-animal protein. Typically, these blends contain two non-animal proteins, three non-animal proteins, four non-animal proteins, or five non-animal proteins. Although more than five non-animal proteins may be used, it is preferred to use two or three non-animal proteins.

[0042] The disclosed non-animal protein blends include proteins from at least two groups as shown in Table 2. For example, the blend can include non-animal proteins from Group 1 and Group 2, Group 1 and Group 3, Group 1 and Group 4, Group 1 and Group 5, Group 1 and Group 6, Group 2 and Group 3, Group 2 and Group 4, Group 2 and Group 5, Group 2 and Group 6, Group 3 and Group 4, Group 3 and Group 5, Group 3 and Group 6, Group 4 and Group 5, Group 4 and Group 6, or Group 5 and Group 6, wherein any of these combinations can optionally include one or more additional proteins from Group 1, Group 2, Group 3, Group 4, Group 5 and / or Group 6, as long as the PDCAAS of the blend is 1.

[0043] Table 2: Groups of non-animal proteins

[0044]

[0045]

[0046] In one aspect, a non-animal protein blend comprising a bean protein and one or more of a synthetic protein, a seed protein, a nut protein, and a grain protein is disclosed, wherein the PDCAAS of the blend is 1. In a particular example, the bean protein can be chickpea protein, pea protein, lentil protein, mung bean protein, soy protein, pinto bean protein, lupin protein, carob protein, or any combination thereof. In other examples, seed protein is present and can be canola protein, sacha inchi protein, flaxseed protein, sesame protein, sunflower protein, pumpkin protein, or any combination thereof. In a particular example, nut protein is present and can be almond protein, walnut protein, cashew protein, hazelnut protein, pecan protein, pistachio protein, or any combination thereof. In a particular example, grain protein is present and can be oat protein, rice protein, barley protein, quinoa protein, wheat protein, corn protein, rye protein, or any combination thereof.

[0047] In one aspect, a non-animal protein blend comprising legume protein, beta-lactoglobulin, and optionally a cereal protein is disclosed. In particular examples, the legume protein can be chickpea protein, pea protein, or a combination of both. In other examples, the cereal protein can be oat protein.

[0048] In one aspect, non-animal protein blends are disclosed that include legume protein, seed protein, and optionally grain protein. In a particular example, the legume protein can be chickpea protein. In a particular example, the seed protein can be canola protein. In other examples, the seed protein is sacha inchi protein. In other examples, the grain protein can be oat protein.

[0049] In one aspect, a non-animal protein blend comprising ovomucoid, legume protein, seed protein, and optionally grain protein is disclosed. In a particular example, the legume protein can be chickpea protein. In other examples, the seed protein can be sacha inchi protein. In other examples, the grain protein can be oat protein.

[0050] In one aspect, a non-animal protein blend is disclosed that includes about 25 wt.% to about 50 wt.% legume protein, about 41 wt.% to about 50 wt.% synthetic protein, and about 0 wt.% to about 34 wt.% cereal protein. In a particular example, the legume protein can be chickpea protein. In other examples, the cereal protein can be oat protein.

[0051] In one aspect, a non-animal protein blend is disclosed, comprising 20wt.% to about 87wt.% legume protein, about 14wt.% to about 37wt.% synthetic protein, and about 0wt.% to about 26wt.% cereal protein. In a particular example, the legume protein can be chickpea protein, pea protein, or a combination of the two. In some examples, the blend can include about 0wt.% to about 43wt.% chickpea protein. In other examples, the cereal protein can be oat protein.

[0052] In one aspect, a non-animal protein blend is disclosed that includes about 55 wt.% to about 60 wt.% legume protein, about 40 wt.% to about 45 wt.% seed protein, and about 0 wt.% to about 1 wt.% grain protein. In a particular example, the legume protein can be chickpea protein. In other examples, the seed protein can be canola protein. In other examples, the grain protein can be oat protein.

[0053] In one aspect, a non-animal protein blend is disclosed that includes about 1 wt.% to about 65 wt.% legume protein and about 33 wt.% to about 45 wt.% seed protein. In particular examples, the legume protein can be chickpea protein, pea protein, lentil protein, or any combination thereof. In particular examples, the blend can include about 0 wt.% to about 53 wt.% chickpea protein, about 1 wt.% to about 65 wt.% pea protein, and / or about 0 wt.% to about 25 wt.% lentil protein. In other examples, the seed protein can be sacha inchi protein.

[0054] In one aspect, a non-animal protein blend is disclosed that includes about 1 wt.% to about 29 wt.% synthetic protein, about 37 wt.% to about 92 wt.% legume protein, about 7 wt.% to about 34 wt.% seed protein, and about 0 wt.% to about 1 wt.% grain protein. In a particular example, the legume protein can be chickpea protein. In other examples, the seed protein can be sacha inchi protein. In other examples, the grain protein can be oat protein.

[0055] In all non-animal protein blends disclosed herein, these blends do not contain animal proteins, such as milk proteins such as whey protein or casein. In other examples, the non-animal protein blends disclosed herein are substantially free of animal proteins.

[0056] Proteins suitable for use herein can be derived from single proteins, concentrates or isolates of plants. Some can also come from precision fermentation, cell culture methods, plant genetic engineering methods, fermented microorganisms.

[0057] drinks

[0058] Also disclosed herein are nutritional beverages comprising a non-animal protein blend having a PDCAAS of 1 as described herein. In particular aspects, the provided beverages comprise from about 1 gram to about 14 grams of protein per about 2 to about 32 fluid ounces (e.g., from about 4 to about 24 fluid ounces, from about 6 to about 18 fluid ounces, from about 10 to about 16 fluid ounces, or about 12 fluid ounces). The beverage can be 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, or 32 fluid ounces, wherein any of these values ​​may form the upper or lower endpoint of a range. In particular aspects, the beverages provided contain from about 1 gram to about 14 grams, from about 2 grams to about 12 grams, from about 3 grams to about 10 grams, or from about 5 grams to about 8 grams per 8 ounce serving, or greater than about 1 gram, about 2 grams, about 3 grams, about 4 grams, about 5 grams, about 6 grams, about 7 grams, about 8 grams, about 9 grams, about 10 grams, about 11 grams, about 12 grams, about 13 grams, or about 14 grams per 8 ounce serving, any of which may form the upper or lower endpoint of a range.

[0059] The nutritious beverage disclosed herein can also include water.Water can exist with about 80% to about 99.9% of beverage weight, about 89% to about 99%, about 88% to about 98% or about 87% to about 97% level, or with about 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or about 99% level of beverage weight, wherein any one of these values ​​can form the upper end point or lower end point of scope.Nutritious beverage can be instant liquid preparation, frozen instant drink, coffee beverage (for example, green coffee and / or roasted coffee), tea beverage, powdered beverage, liquid concentrate, flavor water, fruit juice and fruit juice flavor beverage.In some instances, beverage is non-dairy beverage.

[0060] Specific amino acids that may be present in the disclosed beverages include, but are not limited to: histidine, which may be present in an amount of at least about 4 mg / g, at least about 6 mg / g, at least about 8 mg / g, or at least about 10 mg / g; isoleucine, which may be present in an amount of at least about 6 mg / g, at least about 7 mg / g, at least about 8 mg / g, or at least about 9 mg / g; leucine, which may be present in an amount of at least about 14 mg / g, at least about 16 mg / g, at least about 17 mg / g, or at least about 18 mg / g; lysine, which may be present in an amount of at least about 10 mg / g, at least about 11 mg / g, at least about 12 mg / g, or at least about 13 mg / g; methionine, which may be present in an amount of at least about 2 mg / g; / g, at least about 3 mg / g, at least about 4 mg / g, or at least about 5 mg / g; phenylalanine, which can be present in an amount of at least about 6 mg / g, at least about 7 mg / g, at least about 8 mg / g, or at least about 9 mg / g; threonine, which can be present in an amount of at least about 10 mg / g, at least about 12 mg / g, at least about 14 mg / g, or at least about 16 mg / g; tryptophan, which can be present in an amount of at least about 0.1 mg / g, at least about 1 mg / g, at least about 1.5 mg / g, or at least about 2.0 mg / g; and valine, which can be present in an amount of at least about 10 mg / g, at least about 12 mg / g, at least about 14 mg / g, or at least about 16 mg / g.

[0061] food

[0062] Also disclosed is a food product comprising a non-animal protein blend having a PDCAAS of 1 as described herein. A food product can be any product that can be consumed by a subject (e.g., eaten or drunk). Examples of suitable food products that can contain the disclosed non-animal blend are candy, chewing gum, soft candy, chewing gum, bread, cereals, and snack bars. Food can contain 1 gram to about 14 grams, about 2 grams to about 12 grams, about 3 grams to about 10 grams, or about 5 grams to about 8 grams per 8 ounce serving or greater than about 1 gram, about 2 grams, about 3 grams, about 4 grams, about 5 grams, about 6 grams, about 7 grams, about 8 grams, about 9 grams, about 10 grams, about 11 grams, about 12 grams, about 13 grams, or about 14 grams per 8 ounce serving, wherein any one of the values ​​can form an upper or lower endpoint of a range.

[0063] method

[0064] Also disclosed herein is a method of providing protein to an individual, comprising administering to the individual a nutritional beverage as disclosed herein and containing from about 2 grams to about 10 grams of a non-animal protein blend as disclosed herein. In a particular example, an individual may administer 4 fluid ounces, 8 fluid ounces, 12 fluid ounces, 16 fluid ounces, 20 fluid ounces, 24 fluid ounces, 28 fluid ounces, 32 fluid ounces, 36 fluid ounces, 40 fluid ounces, 44 fluid ounces, 48 ​​fluid ounces, 52 fluid ounces, 56 fluid ounces, or 60 fluid ounces of the disclosed nutritional beverage in a given day.

[0065] Additional components

[0066] The disclosed non-animal blend or the composition (e.g., beverage, food, supplement) comprising it can contain additional ingredients, such as fiber, prebiotics, probiotics, digestive enzymes, sweeteners, flavorings, coloring agents, preservatives, vitamins and minerals. Suitable examples of fiber include water-soluble fibers, such as alginate, β-glucan, carrageenan, cellulose, chitosan, chitin, hydrolyzed guar gum, galacto-oligosaccharides, hemicellulose, hydroxymethylcellulose, hydroxypropylmethylcellulose, inulin, inulin, resistant maltodextrin, mucus, pectin, psyllium husk, polydextrose, xanthan gum, plant gum fiber (e.g., guar gum, gum arabic, etc.). Fiber can be present in the final composition with about 0.01wt.% to about 15wt.% of the total composition.

[0067] Prebiotics are oligosaccharides that serve as a source of energy for probiotics and promote their growth. Examples of suitable prebiotics are fructooligosaccharides, galacto-oligosaccharides, inulin and xylo-oligosaccharides.

[0068] Probiotics are live microorganisms that enhance the microbial environment of the human digestive tract and promote digestive health. Examples of suitable probiotics are bifidobacteria, such as strains of B. adolescentis, B. animalis, B. bifidum, B. longum, B. thermophilwn; Lactobacilli, such as strains of L. acidophilus, L. brevis, L. casei, L. bulgaricus, L. fermentum, L. GG, L. lactis, L. plantarum, L. reuteri, L. rhamnosus, L. salivarius; Bacillus cereus toyoi, Bacillus cereus, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cerevisiae, L. cereus), Bacillus coagulans; Streptococci, such as S. cremoris, S. infantarius, S. intermedius, and S. lactis strains.

[0069] To aid digestive health and nutrient absorption, additional additives in nutritional powdered protein shakes are digestive enzymes such as amylase, lipase, protease, cellulase, alpha-galactosidase, amyloglucosidase, beta-glucanohydrolase, bromelain, glucanase, pepsin, hemicellulase, invertase, trypsin, pectinase, papain, peptidase and xylanase.

[0070] Exemplary sweeteners that can be added include nutritive and / or non-nutritive sweeteners such as cane sugar, syrup, tapioca syrup, honey, HFCS ("glucose syrup"), FIS ("fully inverted sugar"), MIS ("medium inverted sugar"), erythritol, aspartame, acesulfame potassium (Ace-K), steviol glycosides (e.g., Reb A, Reb M), sucralose, saccharin, psicose, glucose, sucrose, stevia, juice or extract, or combinations thereof.

[0071] Exemplary flavorings that may be suitable for use in the disclosed blends and compositions include coffee flavoring, jasmine tea flavoring, berry (e.g., pomegranate-blueberry, mixed berry, etc.) flavoring, citrus (e.g., lemon, lemon-grapefruit, etc.) flavoring, fruit punch flavoring, spice (e.g., ginger) flavoring, etc. Certain formulations do not have added flavorings. If desired, preservatives may be added, depending on the other ingredients, production techniques, desired shelf life, etc. Optionally, caffeine may be added.

[0072] Exemplary preservatives that can be used in the disclosed blends and compositions include benzoates (e.g., sodium benzoate, calcium benzoate, and potassium benzoate), sorbates (e.g., calcium sorbate and potassium sorbate), citrates (e.g., sodium citrate and potassium citrate), polyphosphates (e.g., sodium hexametaphosphate (SHMP)), and mixtures thereof. Antioxidants (e.g., ascorbic acid, EDTA, BHA, BHT, TBHQ, dehydroacetic acid, dimethyl dicarbonate, ethoxyquinoline, heptyl paraben, and combinations thereof) can also be included in the disclosed blends.

[0073] Specific vitamins that may be present in the disclosed blends and compositions include, but are not limited to, vitamin A, vitamin C, B1 (thiamine), B2 (riboflavin), B3 (niacin), B6 ​​(pyridoxine), vitamin D, and folic acid.

[0074] Specific minerals that may be present in the disclosed blends and compositions include, but are not limited to, calcium, potassium, magnesium, manganese, sodium, phosphorus, or zinc.

[0075] Examples

[0076] The following examples are presented to provide a person of ordinary skill in the art with a complete disclosure and description of how to prepare and evaluate the compounds, compositions, articles, devices and / or methods claimed herein, and are intended only as examples of the present invention and are not intended to limit the scope of their invention as the inventors believe. However, those skilled in the art will appreciate from this disclosure that many changes may be made to the specific embodiments disclosed and still obtain the same or similar results without departing from the spirit and scope of the present invention.

[0077] Efforts have been made to ensure numerical accuracy, but some errors and deviations should be accounted for. Unless indicated otherwise, parts are parts by weight, temperature is in °C or is at ambient temperature, and pressure is at or near atmospheric.

[0078] Example 1-17:

[0079] The following combination of non-animal proteins was found to have a PDCAAS of 1.

[0080] Table 3: Examples of non-animal protein blends

[0081]

[0082]

[0083] Taste test:

[0084] Examples 14, 15 and 17 and commercially available pea protein as a control were subjected to taste testing.

[0085]

Claims

1. A non-animal protein blend comprising soy protein and one or more of synthetic protein, seed protein, nut protein, and grain protein, wherein the PDCAAS of the blend is 1.

2. The blend of claim 1, comprising the soy protein, the synthetic protein and optionally the cereal protein.

3. The blend of claim 1, comprising the pulse protein, the synthetic protein, the seed protein and optionally the grain protein.

4. The blend of claim 1 comprising the pulse protein, seed protein and optionally grain protein.

5. The blend of any one of the preceding claims, wherein the legume protein is chickpea protein, pea protein, lentil protein, mung bean protein, soy protein, pinto bean protein, lupin protein, carob protein, or any combination thereof.

6. The blend of any one of the preceding claims, wherein the seed protein is present and is canola protein, sacha inchi protein, flaxseed protein, sesame protein, sunflower protein, pumpkin protein, or any combination thereof.

7. A blend as claimed in any one of the preceding claims, wherein the nut protein is present and is almond protein, walnut protein, cashew protein, hazelnut protein, pecan protein, pistachio protein or any combination thereof.

8. The blend of any one of the preceding claims, wherein the cereal protein is present and is oat protein, rice protein, barley protein, quinoa protein, wheat protein, corn protein, rye protein, or any combination thereof.

9. The blend of claim 1 comprising about 25 wt.% to about 50 wt.% soy protein, about 41 wt.% to about 50 wt.% synthetic protein, and about 0 wt.% to about 34 wt.% grain protein.

10. The blend of claim 9, wherein the legume protein is chickpea protein, the synthetic protein is beta-lactoglobulin, and the cereal protein is oat protein.

11. The blend of claim 1 comprising about 20 wt.% to about 87 wt.% soy protein, about 14 wt.% to about 37 wt.% synthetic protein, and about 0 wt.% to about 26 wt.% grain protein.

12. The blend of claim 11, comprising from about 0 wt.% to about 43 wt.% chickpea protein, from about 20 wt.% to about 87 wt.% pea protein, and the cereal protein is oat protein.

13. The blend of claim 1 comprising about 55 wt.% to about 60 wt.% pulse protein, about 40 wt.% to about 45 wt.% seed protein, and about 0 wt.% to about 1 wt.% grain protein.

14. The blend of claim 13 wherein the legume protein is chickpea protein, the seed protein is canola protein, and the grain protein is oat protein.

15. The blend of any one of claims 11-14, wherein the synthetic protein is β-lactoglobulin.

16. The blend of claim 3 comprising from about 1 wt.% to about 65 wt.% soy protein and from about 33 wt.% to about 45 wt.% seed protein.

17. The blend of claim 16 comprising about 0 wt.% to about 53 wt.% chickpea protein, about 1 wt.% to about 65 wt.% pea protein and / or about 0 wt.% to about 25 wt.% lentil protein, and the seed protein is sacha inchi protein.

18. The blend of claim 3 comprising about 1 wt.% to about 29 wt.% ovomucoid, about 37 wt.% to about 92 wt.% legume protein, about 7 wt.% to about 34 wt.% seed protein, and about 0 wt.% to about 1 wt.% grain protein.

19. The blend of claim 18, wherein the pulse protein is chickpea protein, the seed protein is sacha inchi protein, and the grain protein is oat protein.

20. The blend of any one of the preceding claims, wherein the blend is free of animal protein.

21. The blend of any one of the preceding claims, further comprising one or more of fiber, prebiotics, probiotics, digestive enzymes, sweeteners, flavorings, colorants, preservatives, vitamins, and minerals.

22. The blend of any one of the preceding claims, wherein the blend has one or more of improved taste, aroma or mouthfeel.

23. A beverage comprising the blend according to any one of claims 1 to 22.

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