Ultra-long-distance pig clone embryo transportation device and use method

Abstract

The present invention relates to the technical field of cloned embryo transportation, and discloses an ultra-long-distance pig cloned embryo transportation device and a method for using the device, wherein the device comprises: a first storage box and a second storage box, which are used to accommodate a second layer of absorbent cotton and provide additional buffering protection to enhance the stability of the embryo during transportation; a buffer plate, which is arranged in the first storage box, and is used to buffer the vibration generated during transportation; absorbent cotton, which is used to wrap a storage body and provide a moist and heat-insulating environment; and a storage body, which is shaped like an inverted gourd, and is used to store pig cloned embryos and maintain the stability of a phosphate buffer system, and regulate the environment and pH value during transportation. The storage body uses a phosphate buffer solution, which effectively regulates the CO2 concentration and pH value during transportation, provides a stable growth environment for the embryo, and avoids the risk of environmental fluctuations during transportation to the embryo.

Description

Technical Field

[0001] The present invention relates to the technical field of cloned embryo transportation, and in particular to an ultra-long-distance pig cloned embryo transportation device and a use method thereof. Background Art

[0002] With the rapid development of modern agriculture and biotechnology, pig cloning technology has become an important research direction, especially in the field of in vitro fertilization technology and embryo transplantation. The survival and transportation of pig cloned embryos have always been key factors restricting their application and promotion. Due to the fragility of pig cloned embryos during in vitro culture, embryo transportation needs to face multiple challenges such as temperature and humidity control, gas environment regulation, and vibration protection. How to effectively protect the survival of embryos during long-term and ultra-long-distance transportation has become a technical problem that needs to be solved urgently.

[0003] At present, the existing pig clone embryo transportation devices mostly use traditional plastic containers, foam boxes or simple moist cotton wool packaging. These devices are relatively simple in design and mainly rely on the natural temperature and humidity regulation of the external environment. They lack effective temperature, humidity and CO2 concentration control, and the impact of vibration and external shock on the embryo during transportation is not effectively alleviated. In addition, traditional transportation devices usually use standard containers for simple packaging, which fails to provide adequate protection for the embryo. Especially during long-term transportation, the embryo survival rate and transplantation success rate are seriously affected.

[0004] In the prior art, the traditional device lacks an effective environmental control system and cannot adjust the temperature, humidity and CO2 concentration during transportation, which results in the embryo being unable to maintain an ideal growth environment during transportation, resulting in a low survival rate and a poor transplantation success rate. This problem is the main defect of the prior art. The present invention is aimed at this problem and proposes an ultra-long-distance pig clone embryo transportation device with temperature, humidity and CO2 concentration adjustment functions, which can effectively ensure the survival rate of the embryo during transportation and improve the transplantation success rate. Summary of the invention

[0005] In view of the shortcomings of the prior art, the present invention provides an ultra-long-distance pig cloned embryo transportation device and a method of use, which solves the problem that the existing devices are unable to adjust the temperature, humidity and CO2 concentration during transportation, resulting in the embryos being unable to maintain an ideal growth environment during transportation, resulting in a low survival rate and a poor transplantation success rate.

[0006] To achieve the above objectives, the present invention is implemented through the following technical solutions: an ultra-long-distance pig clone embryo transportation device, the device comprising:

[0007] The first storage box and the second storage box are used to contain the second layer of absorbent cotton and provide additional cushioning protection to enhance the stability of the embryo during transportation;

[0008] A buffer plate, arranged in the first storage box, for buffering vibrations generated during transportation;

[0009] Absorbent cotton, used to wrap the storage body and provide a moist and warm environment;

[0010] The storage body is in the shape of an inverted gourd and is used to store pig cloned embryos and maintain the stability of the phosphate buffer system and regulate the environment and pH value during transportation;

[0011] A protective cover is used to seal the storage body to prevent the embryos from being scattered and disturbed by the outside world;

[0012] The fixing ring and the connecting belt are used to fix the first storage box and the second storage box to prevent the position of the storage body from changing during transportation;

[0013] Sealing gasket, used to enhance the sealing of the storage body and prevent gas leakage;

[0014] The connecting ring is used to connect the storage body and the protective cover.

[0015] Preferably, the storage body contains a phosphate buffer solution, which can adjust the CO2 concentration and pH value during transportation, thereby optimizing the survival environment of the embryo.

[0016] Preferably, the first storage box adopts a structural design suitable for stable embryo storage, which can effectively reduce vibration and shaking during transportation.

[0017] Preferably, the cotton wool is moist cotton wool, which has a certain heat preservation function, can maintain the humidity and temperature during transportation, and increase the survival rate of the embryo.

[0018] Preferably, the buffer plate is a multi-layer structure, one layer of which is an elastic material, which is used to effectively buffer the vibration during transportation and reduce the impact of external shocks on the embryo.

[0019] Preferably, the first storage box and the second storage box are connected by an interlocking structure to enhance stability and safety during transportation.

[0020] A method for using an ultra-long-distance pig clone embryo transportation device comprises the following steps:

[0021] Step 1: Place the pig cloned embryo in a storage body and wrap it with moistened absorbent cotton to ensure humidity and temperature during transportation;

[0022] Step 2: Place the storage body into the first storage box and use a buffer plate to buffer the vibration to reduce the impact of vibration on the embryo during transportation;

[0023] Step 3: Seal the storage body with a protective cover to prevent the embryos from scattering and ensure the safety of the embryos during transportation;

[0024] Step 4: Regulate the CO2 concentration and pH value in the storage body through the phosphate buffer system to maintain the ideal environment required for embryo survival;

[0025] Step 5: Use a fixing ring and a connecting belt to ensure a stable connection between the first storage box and the second storage box to prevent position changes during transportation;

[0026] Step 6: After the transport is completed, check the survival of the embryos, evaluate the transport and prepare for the transplant operation.

[0027] Preferably, the steps further include:

[0028] After the first storage box and the second storage box are connected and fixed, the stability of the entire device during transportation is ensured by the fixing ring and the connecting belt;

[0029] During transportation, the position of the storage body is continuously monitored to avoid changes in the position of the embryos due to vibration during transportation.

[0030] Preferably, the method further comprises:

[0031] Regularly check the temperature and humidity in the storage chamber and adjust the moisture level of the moistened cotton wool to ensure that the embryos are always in the best environment during long-term transportation.

[0032] During transportation, external temperature and humidity control equipment is used to assist in adjusting the device to maintain stable transportation conditions.

[0033] Preferably, the method is suitable for long-term and ultra-long-distance transportation, ensuring that the embryos are in a stable CO2 temperature and humidity environment and conditions, thereby effectively improving the embryo survival rate and transplantation success rate.

[0034] The present invention provides an ultra-long-distance pig clone embryo transportation device and a method of use. It has the following beneficial effects:

[0035] 1. The present invention effectively mitigates the impact of vibration and external shock on the embryo during transportation through the multi-layer structure design of the buffer plate and elastic material, and reduces the damage to the embryo caused by vibration. The storage body adopts phosphate buffer solution, which effectively regulates the CO2 concentration and pH value during transportation, provides a stable growth environment for the embryo, and avoids the risk of environmental fluctuations during transportation.

[0036] 2. The use of the moistened absorbent cotton of the present invention provides continuous humidity and temperature control for the embryo, maintains a stable environment during transportation, and thus significantly improves the survival rate of the embryo. During long-term transportation, the moistened absorbent cotton effectively prevents the embryo from being damaged due to dryness or excessive moisture, ensuring that the embryo is in an ideal protective environment during transportation.

[0037] 3. The present invention is particularly suitable for long-term and ultra-long-distance transportation of pig cloned embryos. By using moistened absorbent cotton and a phosphate buffer system, the required temperature and humidity environment and stable CO2 conditions can be provided for the embryos. Even during long-term transportation, the survival rate of the embryos can be effectively guaranteed, overcoming the problem of embryo death caused by the lack of environmental control in traditional transportation methods.

[0038] 4. The present invention reduces the reliance on complex equipment during transportation by using simple and easily available materials to wet absorbent cotton and phosphate buffer solution, which can ensure the survival and safety of the embryos and reduce the cost during transportation. At the same time, the design of the fixing ring and the connecting belt makes it difficult for the device to change position during transportation, thereby improving transportation efficiency.

[0039] 5. The device of the present invention is suitable for various transportation scenarios, especially for intra-provincial, inter-provincial and ultra-long-distance transportation. It has a simple structure and is easy to operate. Transport personnel can flexibly adjust environmental control and protection measures according to needs to ensure the safety of embryos throughout the transportation process. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 is a flow chart of the method of the present invention;

[0041] Figure 2 A perspective view of the present invention;

[0042] Figure 3 It is a schematic diagram of the partial structure of the absorbent cotton of the present invention;

[0043] Figure 4 It is a schematic diagram of the structure of the storage body part of the present invention.

[0044] Among them, 1. a first storage box; 2. a buffer plate; 3. cotton wool; 4. a storage body; 5. a protective cover; 6. a fixing ring; 7. a connecting belt; 8. a sealing gasket; 9. a connecting ring; 10. a second storage box. DETAILED DESCRIPTION

[0045] The following will be combined with the drawings in the specification of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0046] Please refer to the attached Figure 2 -Attached Figure 3 The embodiment of the present invention provides an ultra-long distance pig clone embryo transportation device, the device comprising:

[0047] The first storage box 1 and the second storage box 10 are used to contain the second layer of absorbent cotton and provide additional cushioning protection to enhance the stability of the embryo during transportation;

[0048] A buffer plate 2 is arranged in the first storage box 1 and is used to buffer vibrations generated during transportation;

[0049] Absorbent cotton 3, used to wrap the storage body 4 and provide a moist and heat-insulating environment;

[0050] The storage body 4 is in the shape of an inverted gourd and is used to store the pig cloned embryos and maintain the stability of the phosphate buffer system and adjust the CO2 environment and pH value during transportation;

[0051] A protective cover 5, used to seal the storage body 4 to prevent the embryos from scattering and external interference;

[0052] The fixing ring 6 and the connecting belt 7 are used to fix the first storage box 1 and the second storage box 10 to prevent the position of the storage body 4 from changing during transportation;

[0053] A sealing gasket 8, used to enhance the sealing performance of the storage body 4 and prevent gas leakage;

[0054] The connecting ring 9 is used to connect the storage body 4 and the protective cover 5 .

[0055] In one embodiment, the first storage box 1 and the second storage box 10 adopt an interlocking structure to ensure the stability and reliability of the device during transportation. The first storage box 1 is used to store the storage body 4, and the second storage box 10 provides a second layer of absorbent cotton 3 for buffering protection, which enhances the embryo to avoid impact and vibration during transportation. The buffer plate 2 is located in the first storage box 1, and adopts a multi-layer design, one of which is an elastic material, which can effectively alleviate the vibration during transportation and prevent vibration from damaging the embryo. The buffer plate 2 also has the function of adjusting the external impact force, reducing the impact of vibration on the embryo. The moist absorbent cotton 3 wraps the storage body 4, and its moistening effect can effectively maintain the humidity and temperature required by the embryo during transportation, preventing the embryo from being damaged by dryness or overheating during transportation. The moist absorbent cotton also has a certain hygroscopicity, which can maintain a stable environment for the embryo during transportation. The storage body 4 has good sealing and stability. The storage body is filled with phosphate buffer solution to adjust the CO2 concentration and pH value during transportation, provide an ideal growth environment required by the embryo, and ensure that the embryo can survive in an environment of hypoxia or lack of annulus. The protective cover 5 is made of transparent plastic or glass material, which has good sealing and pressure resistance. The inner side of the protective cover is designed as a sealable interface to prevent the embryo from contacting the outside air. A safety buckle is also provided on the cover to ensure that the protective cover is not accidentally opened during transportation. The fixing ring 6 and the connecting belt 7 are used to fix the first storage box 1 and the second storage box 10, and the entire device is further stabilized by the elastic connecting belt 7 to avoid position changes during transportation. The connecting belt material is made of high-strength nylon fabric with strong tensile strength. The sealing gasket 8 is made of rubber or EPDM material with good elasticity and sealing performance to prevent gas leakage. The gasket is set at the interface between the storage body 4 and the protective cover 5 to ensure the stability of the CO2 gas. The connecting ring 9 is used to connect the storage body 4 and the protective cover 5 to prevent the protective cover from being displaced by external forces during transportation, thereby ensuring the sealing of the storage body 4 and the safety of the embryo.

[0056] The storage body 4 contains a phosphate buffer solution, which can adjust the CO2 concentration and pH value during transportation, thereby optimizing the survival environment of the embryo; the first storage box 1 adopts a structural design suitable for stable embryo storage, which can effectively reduce vibration and shaking during transportation.

[0057] The buffer plate 2 is a multi-layer structure, one layer of which is made of elastic material, which is used to effectively buffer the vibration during transportation and reduce the impact of external shocks on the embryo.

[0058] The first storage box 1 and the second storage box 10 are connected by an interlocking structure, which enhances the stability and safety during transportation.

[0059] In one embodiment, the phosphate buffer solution filled in the storage body 4 plays a vital role in the transportation process. The solution can continuously adjust the CO2 concentration and pH value in the storage body during transportation, ensuring that the embryo can continue to survive in an environment with hypoxia and low CO2 concentration. The phosphate buffer solution provides a stable acid-base environment to prevent the embryo from being affected by environmental fluctuations and maintain its cell activity; the first storage box 1 ensures that the embryo is in a stable state through its structural design. This structural design works together with the elastic material in the buffer plate 2 to effectively reduce the vibration and shaking during transportation. The elastic material can absorb and disperse the vibration energy when subjected to external impact, reducing the damage to the embryo caused by the impact force. The buffer plate avoids the position displacement or damage of the embryo caused by vibration by dispersing the power of external impact. The phosphate buffer solution in the storage body 4 cooperates with the protective cover 5 through a sealing design to ensure the stability of the environment in which the embryo is located during transportation. The protective cover is tightly combined with the sealing gasket 8 of the storage body to prevent gas leakage, so that the CO2 concentration and pH value can be kept in the optimal range, further improving the survival rate of the embryo. The design of the present invention enables the device to be easily operated before, during and after transportation. The filling of the transport fluid in the storage and transport device and the placement of the embryos are very simple, and the environmental control in the storage body 4 makes the transport process unnecessary for additional complex equipment support. After the transport is completed, only simple inspection and operation are required to ensure the safe transport and efficient transplantation of the embryos.

[0060] Based on the above embodiment, this embodiment also has a method for manufacturing an ultra-long distance pig clone embryo transportation device. The steps of manufacturing the ultra-long distance pig clone embryo transportation device are as follows:

[0061] 1. Material Preparation

[0062] Centrifuge tube materials:

[0063] Take a 600μL centrifuge tube and a 50ml centrifuge tube as the main container for making the storage and transportation device.

[0064] The centrifuge tube is made of food grade polypropylene PP or polyethylene PE material, which has good chemical stability and physical strength and is not easy to react with the transport fluid.

[0065] Wet cotton wool 3:

[0066] Use high-quality cotton wool3 to ensure it is pure and does not release harmful substances.

[0067] The absorbent cotton 3 must be sterilized to ensure its sterility and avoid infection of the embryo during transportation.

[0068] Sterile water:

[0069] Use sterile water or physiological saline to completely soak the absorbent cotton 3, ensure that the humidity of the wet absorbent cotton 3 is moderate, and maintain a stable humidity environment.

[0070] Transport fluid:

[0071] The transport fluid should be prepared according to the embryo type and transport time. It is usually a liquid containing buffer, glucose, amino acids, etc. to maintain the physiological needs of the embryo.

[0072] 2. Soften the centrifuge tube

[0073] Heating to soften:

[0074] Take a 600 μL centrifuge tube and use a drying gun to heat the middle part of the centrifuge tube to soften it.

[0075] The heating temperature should be controlled at 70℃ to 80℃. Pay attention to uniform temperature during heating to soften the middle part of the centrifuge tube and maintain the plasticity of the material.

[0076] Clamp with tweezers and shape:

[0077] Use tweezers to clamp the middle of the softened centrifuge tube, rotate and clamp it to ensure that the softened part is evenly formed.

[0078] Repeat this operation 3 to 4 times to gradually shape the shape of the centrifuge tube. Each time you clamp it, make sure there is a certain amount of space inside the centrifuge tube to allow for subsequent filling with embryos and transport fluid.

[0079] Finished molding:

[0080] After softening, the shape of the centrifuge tube should be cup-shaped or slightly constricted in the middle to provide a stable storage space for the embryos.

[0081] Through the above steps, a cloned embryo storage and transportation device that meets the requirements was successfully produced.

[0082] 3. Fill with moistened cotton wool

[0083] Processing of 50ml centrifuge tubes:

[0084] Take a 50 ml centrifuge tube and open the lid.

[0085] Use tweezers to fill the moistened cotton wool 3 to 2 / 3 of the centrifuge tube, ensuring that the cotton wool 3 is evenly distributed.

[0086] Add sterile water:

[0087] Add an appropriate amount of sterilized water into the 50 ml centrifuge tube to ensure that the absorbent cotton 3 is completely soaked. This step must ensure that the absorbent cotton 3 is completely absorbed by the water so that it can maintain a stable humidity during transportation.

[0088] To fill and fix the centrifuge tube:

[0089] Take 4-5 600μL centrifuge tubes and place them in the 50ml centrifuge tube in turn. After placing each 600μL centrifuge tube, fill it with moistened cotton wool 3 to ensure that each centrifuge tube is stable and does not move.

[0090] When placing the 600 μL centrifuge tube each time, ensure that the cotton wool 3 is filled tightly and evenly to avoid leaving any gaps.

[0091] Add sterile water again:

[0092] Add more sterile water until there is no flowing water, making sure the whole device is moist and not easy to dry. Make sure the sterile water completely penetrates into the absorbent cotton 3 and compacts the absorbent cotton 3.

[0093] 4. Shaping and loading

[0094] Put the lid on:

[0095] Put the lid on the 50ml centrifuge tube, but be careful not to tighten the lid. This will prevent pressure from over-sealing and help allow air to circulate, making the internal temperature and humidity more balanced.

[0096] Place in incubator:

[0097] Place the 50ml centrifuge tube filled with moistened cotton wool in an incubator for shaping. The shaping time is 24 hours, during which the appropriate temperature and humidity should be maintained.

[0098] Setting environment: The temperature should be controlled at around 25°C and the relative humidity should be between 60% and 80%. Adequate ventilation should be maintained during the setting period to avoid air stagnation.

[0099] Post-production inspection:

[0100] After shaping is completed, check whether the moistened absorbent cotton in the storage and transportation device is even and stable, and there is no drying or water accumulation.

[0101] 5. Complete the production of storage and transportation equipment

[0102] Loading embryos:

[0103] After taking the storage and transport device out of the incubator, open the lid and prepare for transporting the cloned embryos.

[0104] Use a mouth pipette or pipette as needed to add the transport solution into the reservoir. Add from the bottom to the top until the entire reservoir is filled.

[0105] Adding cloned embryos:

[0106] Use a mouth pipette to suck the embryos to be transported into the storage and transportation device, and let it stand for 1-2 minutes to allow the embryos to sink to the bottom naturally.

[0107] Observe that the embryos are evenly distributed and ensure that they are stably positioned within the transport device.

[0108] Check the transport device:

[0109] Turn the storage and transport device loaded with embryos and transport fluid upside down to test whether there is any scattering. If there is no scattering, you can continue numbering and proceed to the next step; if there is scattering, replace the storage and transport device.

[0110] Transport preparation:

[0111] Make sure there is no flowing water on the moistened cotton wool inside the device and close the lid tightly. Before transporting, make sure the device is sealed and no liquid will leak.

[0112] It can be carried with you or placed in a transport box for transportation. The device is stable and can adapt to long-term and ultra-long-distance transportation, ensuring that the embryo maintains a stable temperature and humidity and a protective environment during transportation.

[0113] Embodiment 2:

[0114] Please see attached Figure 1 Based on the above embodiments, this embodiment provides a method for using an ultra-long-distance pig clone embryo transportation device, comprising the following steps:

[0115] Step 1: Place the pig cloned embryo into the storage body 4 and wrap it with moistened absorbent cotton 3 to ensure the humidity and temperature of the embryo during transportation;

[0116] Step 2: Place the storage body 4 into the first storage box 1, and use the buffer plate 2 for vibration buffering to reduce the impact of vibration on the embryo during transportation;

[0117] Step 3: Use a protective cover 5 to seal the storage body 4 to prevent the embryos from scattering and ensure the safety of the embryos during transportation;

[0118] Step 4: Regulate the CO2 concentration and pH value in the storage body 4 through a phosphate buffer system to maintain an ideal environment for embryo survival;

[0119] Step 5: Use the fixing ring 6 and the connecting belt 7 to ensure a stable connection between the first storage box 1 and the second storage box 10 to prevent position changes during transportation;

[0120] Step 6: After the transport is completed, check the survival of the embryos, evaluate the transport and prepare for the transplant operation.

[0121] The steps further include:

[0122] After the first storage box 1 and the second storage box 10 are connected and fixed, the fixing ring 6 and the connecting belt 7 are used to ensure the stability of the entire device during transportation;

[0123] During transportation, the position of the storage body 4 is continuously monitored to prevent the embryo position from changing due to vibration during transportation.

[0124] The method further comprises:

[0125] By regularly checking the temperature and humidity in the storage body 4, the degree of moisture of the moistened absorbent cotton 3 is adjusted to ensure that the embryo is always in the best environment during the long-term transportation process;

[0126] During transportation, external temperature and humidity control equipment is used to assist in adjusting the device to maintain stable transportation conditions.

[0127] The method is suitable for long-term and ultra-long-distance transportation, ensuring that the embryos are in a stable CO2 environment and temperature and humidity conditions, thereby effectively improving the embryo survival rate and transplantation success rate.

[0128] In one embodiment, step 1: preparing the transport fluid and storage device

[0129] First, prepare the transport fluid. The transport fluid usually includes an appropriate proportion of physiological saline, buffer such as phosphate buffer solution, and other additives such as nutrient solution or antibiotics to maintain the biological activity and stability of the embryo. The pH value and CO2 concentration of the transport fluid require special attention and should usually be adjusted to values ​​close to the optimal growth conditions for the embryo, for example, pH 7.4, and CO2 concentration maintained at 5%;

[0130] Step 2: Embryo preparation

[0131] Take the pig cloned embryos from the culture dish, use a 200μl pipette to take an appropriate amount of transport liquid, about 200μL, and add it from the bottom of the storage and transportation device to the top, ensuring that the transport liquid fully covers the inner wall and bottom of the storage and transportation device. Next, use a mouth pipette to gently suck the embryos to be transported and suck them into the device. Let it stand for about 1-2 minutes, and the cloned embryos will naturally sink to the bottom.

[0132] Step 3: Ensure embryo storage stability

[0133] At this time, by wrapping the moist absorbent cotton 3 on the storage body 4, the appropriate humidity and temperature are maintained during transportation to ensure that the embryo is not disturbed. When the moist absorbent cotton is wrapped around the embryo, its degree of moisture must be moderate to avoid damage to the embryo due to too low or too high humidity. The moist absorbent cotton maintains the balance of temperature and humidity by absorbing and releasing water.

[0134] Step 4: Place the storage body into the first storage box

[0135] The storage body 4 in which the embryo is placed and wrapped with moist cotton wool is placed in the first storage box 1. The first storage box is designed to be shockproof, pressure-resistant and able to withstand external impact. At this time, the buffer plate 2 provides vibration buffering in the storage box, reduces the impact of vibration on the embryo during transportation, and ensures that the embryo is stably stored in the storage body.

[0136] Step 5: Close the storage volume

[0137] The storage body 4 is closed with a protective cover 5 to prevent the embryos from scattering and ensure the safety of the embryos during transportation. The sealing design of the protective cover prevents air pollution and ensures a stable environment in the storage body during transportation. At the same time, the safety buckle design on the cover can prevent accidental opening during transportation.

[0138] Step 6: Adjust environmental conditions

[0139] The CO2 concentration and pH value in the storage body 4 are adjusted by the phosphate buffer system. Appropriate solutions and environmental control techniques are used to ensure that the environment in the storage body is maintained within a range suitable for embryo survival. Usually, the CO2 concentration in the transport fluid is set to about 5% and the pH value is maintained at about 7.4. The phosphate buffer solution can stabilize the CO2 concentration and pH value to avoid adverse effects on the embryo due to environmental fluctuations during transportation.

[0140] Step 7: Secure the Unit and Prepare for Shipping

[0141] The fixing ring 6 and the connecting belt 7 are used to ensure a stable connection between the first storage box 1 and the second storage box 10 to prevent the device from changing position during transportation. The connecting belt 7 ensures the stability of the device during transportation through its strong tensile strength.

[0142] Step 8: Continuous monitoring during transportation

[0143] During transportation, the temperature and humidity in the storage body 4 are continuously monitored to ensure that the environment in the storage and transportation device is always in a state suitable for the survival of the embryo. At the beginning of transportation, the temperature, humidity and CO2 concentration in the storage body 4 are checked and adjusted as needed. During long-term transportation, ensuring the stability of temperature and humidity is crucial for the survival of the embryo. If necessary, an external temperature and humidity control device can be used to assist in adjusting the device to ensure stable conditions during transportation.

[0144] Step 9: Inspection and evaluation after transportation

[0145] After the transportation is completed, the storage and transportation device is taken out, the protective cover 5 is opened and the survival of the embryo is checked. If the embryo has not been scattered during transportation and is alive and healthy, the transplant operation can be prepared. If the embryo survival rate is found to be unsatisfactory or abnormal, it should be recorded and improved in time. The survival rate can be improved by further analyzing whether there are problems with the transportation environment control or adjusting the transportation method.

[0146] Step 10: Packaging and Boxing

[0147] Place the storage body 4 into the finalized loading device. At this time, ensure the sealing and stability of the device, and further ensure that there is no flowing water on the wet absorbent cotton in the loading device. Check whether the loading device is well sealed to ensure that moisture does not leak out. Close the lid of the loading device and prepare to start transportation. At this time, you can choose to carry it with you or transport it in a box, depending on the transportation distance and environment.

[0148] Comparative Example 1:

[0149] Traditional storage and transportation equipment:

[0150] Existing transport devices are usually made of standard plastic containers such as 100ml plastic bottles or foam boards to hold cloned embryos. Although such devices can ensure basic stability during short-distance transportation, they lack fine-tuning of vibration, temperature, humidity and CO2 environment.

[0151] Production method: Ordinary plastic bottles or foam boxes are usually used, and moistened cotton wool or foam materials are simply placed in them for packaging, while temperature, humidity and gas control are ignored.

[0152] Functional defects: This device lacks an adjustable CO2 concentration control system, and the temperature and humidity are unstable, which often leads to a low survival rate of embryos after long-term transportation.

[0153] The storage and transportation device of the present invention adopts 600μL centrifuge tubes and 50ml centrifuge tubes as basic containers, and finely adjusts the shape of the containers by heating with a drying gun and clamping with tweezers, so as to produce a more precise and stable storage and transportation device. The multi-layer structure: through the design of a buffer plate 2 and a multi-layer moistened absorbent cotton 3, it is ensured that the embryos are not affected by external shocks and vibrations during transportation.

[0154] Temperature, humidity and gas control: Storage body 4 uses phosphate buffer solution to adjust CO2 concentration and pH value to provide a stable living environment for the embryo.

[0155] Experimental design and results:

[0156] Experimental group: The ultra-long-distance pig clone embryo transportation device of the present invention was used, the embryo was wrapped in moist absorbent cotton 3, the storage body 4 contained phosphate buffer solution, and the buffer plate 2 was used for shock absorption.

[0157] Control group: A traditional pig cloned embryo transport device was used, the embryos were wrapped in ordinary moist cotton wool, no phosphate buffer solution was used, and there was no buffer design.

[0158] Experimental steps:

[0159] The pig cloned embryos were placed in transport devices for the experimental and control groups;

[0160] Long-term transport began for 48 hours, with the temperature, humidity and CO2 concentration in the storage body recorded every 6 hours;

[0161] After transport, the embryos are checked for viability and health.

[0162] Experimental results:

[0163] Experimental Group:

[0164] The embryo survival rate was 98%, all embryos remained healthy and were successfully transplanted. The environmental control and buffer design during transportation effectively ensured the survival conditions of the embryos during long-term transportation.

[0165] Control group:

[0166] The embryo survival rate was only 65%, and many embryos suffered from frostbite and hypoxia, leading to transplantation failure.

[0167]

[0168] Temperature, humidity and CO2 control:

[0169] The device of the present invention uses moistened absorbent cotton (3) and phosphate buffer solution (storage body 4) to adjust temperature, humidity and CO2 concentration, and can always maintain a stable environment required by the embryo during transportation.

[0170] Traditional devices have large fluctuations in temperature, humidity and CO2 concentration, which significantly reduces the survival rate of embryos during transportation.

[0171] Embryo survival rate:

[0172] When the experimental group used the device of the present invention, the embryo survival rate was 98%, while when the control group used the traditional device, the survival rate was only 65%.

[0173] The device of the present invention can significantly improve the survival rate and reduce the embryo loss during transportation.

[0174] Environmental control during transportation:

[0175] During the transportation process of the device of the present invention, the temperature, humidity and CO2 concentration are all stably adjusted to ensure that the embryos are not affected by environmental changes during the transportation process.

[0176] Traditional devices do not have adequate control over the environment, and the environment fluctuates greatly during transportation, resulting in a low embryo survival rate.

[0177] Through experimental comparison, it can be seen that the ultra-long-distance pig clone embryo transportation device of the present invention has obvious technical advantages over the traditional device. Through optimized buffer protection, multi-layer wet absorbent cotton design and the use of phosphate buffer solution, the present invention can effectively improve the survival rate of embryos during long-term and ultra-long-distance transportation, reduce the negative effects of vibration, temperature and humidity and CO2 fluctuations, and greatly improve transportation efficiency and transplantation success rate.

[0178] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An ultra-long distance pig clone embryo transportation device, characterized in that: The device comprises: The first storage box (1) and the second storage box (10) are used to contain the second layer of absorbent cotton and provide additional cushioning protection to enhance the stability of the embryo during transportation; A buffer plate (2) is arranged in the first storage box (1) and is used to buffer vibrations generated during transportation; Absorbent cotton (3), used for wrapping the storage body (4) and providing a moist and heat-insulating environment; The storage body (4) is in the shape of an inverted gourd and is used to store the pig cloned embryos and maintain the stability of the phosphate buffer system and regulate the CO2 environment and pH value during transportation; A protective cover (5) for sealing the storage body (4) to prevent the embryos from being scattered and disturbed by the outside world; The fixing ring (6) and the connecting belt (7) are used to fix the first storage box (1) and the second storage box (10) to prevent the position of the storage body (4) from changing during transportation; A sealing gasket (8) for enhancing the sealing performance of the storage body (4) and preventing gas leakage; The connecting ring (9) is used to connect the storage body (4) and the protective cover (5).

2. The ultra-long distance pig clone embryo transportation device according to claim 1, characterized in that: The storage body (4) contains a phosphate buffer solution, which can adjust the CO2 concentration and pH value during transportation, thereby optimizing the survival environment of the embryo.

3. The ultra-long distance pig clone embryo transportation device according to claim 1, characterized in that: The first storage box (1) adopts a structural design suitable for stable embryo storage, and can effectively reduce vibration and shaking during transportation.

4. The ultra-long distance pig clone embryo transportation device according to claim 1, characterized in that: The absorbent cotton (3) is moist absorbent cotton, has a certain heat preservation function, can maintain the humidity and temperature during transportation, and increase the survival rate of the embryo.

5. The ultra-long distance pig clone embryo transportation device according to claim 1, characterized in that: The buffer plate (2) is a multi-layer structure, one layer of which is made of elastic material, and is used to effectively buffer vibrations during transportation and reduce the impact of external shocks on the embryo.

6. The ultra-long distance pig clone embryo transportation device according to claim 1, characterized in that: The first storage box (1) and the second storage box (10) are connected via an interlocking structure, thereby enhancing stability and safety during transportation.

7. A method for using an ultra-long distance pig clone embryo transportation device, according to the ultra-long distance pig clone embryo transportation device according to any one of claims 1 to 6, characterized in that: The following steps are involved: Step 1: Place the pig cloned embryo in a storage body (4) and wrap it with moistened absorbent cotton (3) to ensure humidity and temperature of the embryo during transportation; Step 2: placing the storage body (4) into the first storage box (1), and using the buffer plate (2) to perform vibration buffering to reduce the impact of vibration on the embryo during transportation; Step 3: Use a protective cover (5) to seal the storage body (4) to prevent the embryos from scattering and ensure the safety of the embryos during transportation; Step 4: Regulating the CO2 concentration and pH value in the storage body (4) through a phosphate buffer system to maintain an ideal environment for embryo survival; Step 5: Use a fixing ring (6) and a connecting belt (7) to ensure a stable connection between the first storage box (1) and the second storage box (10) to prevent position changes during transportation; Step 6: After the transport is completed, check the survival of the embryos, evaluate the transport and prepare for the transplant operation.

8. The method for using the ultra-long distance pig clone embryo transportation device according to claim 7, characterized in that: The steps further include: After the first storage box (1) and the second storage box (10) are connected and fixed, the stability of the entire device during transportation is ensured by using the fixing ring (6) and the connecting belt (7); During the transportation process, the position of the storage body (4) is continuously monitored to prevent the embryo position from changing due to vibration during transportation.

9. The method for using the ultra-long distance pig clone embryo transportation device according to claim 7, characterized in that: The method further comprises: By regularly checking the temperature and humidity in the storage body (4), the moisture level of the moistened absorbent cotton (3) is adjusted to ensure that the embryo is always in the best environment during the long-term transportation process; During transportation, external temperature and humidity control equipment is used to assist in adjusting the device to maintain stable transportation conditions.

10. The method for using the ultra-long distance pig clone embryo transportation device according to claim 7, characterized in that: The method is suitable for long-term and ultra-long-distance transportation, ensuring that the embryos are in a stable CO2 environment and temperature and humidity conditions, thereby effectively improving the embryo survival rate and transplantation success rate.

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