Application of azelaic acid in promoting plant seed germination and / or plant growth

Abstract

The invention provides application of azelaic acid in promoting plant seed germination and / or plant growth, and belongs to the technical field of plant growth regulation. The invention not only provides the application of azelaic acid in promotion of plant seed germination and / or plant growth, but also provides a method for promotion of plant seed germination and / or plant growth, the plant seeds are treated with the azelaic acid solution with the concentration of 0.1-1.0 mM and then sown, and the result of the embodiment shows that the azelaic acid solution with the concentration of 0.1-1.0 mM can be used for promoting plant seed germination and / or plant growth. Azelaic acid can effectively improve the germination rate of seeds under salt stress and normal conditions, shorten the seed germination time, promote the growth of hypocotyl and root of seedlings, and relieve the adverse effects of salt stress environment on seed germination and plant growth.

Description

Technical Field

[0001] The invention belongs to the technical field of agricultural planting, and specifically relates to the application of azelaic acid in promoting plant seed germination and / or plant growth. Background Art

[0002] Soil salinization is the most common problem in agricultural production worldwide. The ion toxicity and osmotic stress it produces have a significant impact on plant growth and development, directly affecting yield and quality. Salinized land is widely distributed in my country, with a total area of ​​about 100 million hectares. It mainly occurs in arid, semi-arid and semi-humid areas. In addition to naturally formed saline-alkali land, people's abuse of fertilizers and pesticides and improper farming methods can also cause the accumulation of salt in the tillage layer, leading to the formation of secondary salinization of the land.

[0003] When the soil salt content exceeds the tolerance range of plants, it will seriously hinder plant growth and development, especially in the germination (emergence) and seedling stages, which are more sensitive to salt concentration. Salt stress is an important factor affecting the ecological limitation of seed germination. At present, breeding salt-tolerant crops is a feasible solution, but there are not many successful examples. Promoting the growth of existing plants and seed germination has become an effective strategy. Therefore, how to improve plant growth and seed germination is an urgent problem to be solved.

[0004] Azelaic acid, also known as azelaic acid, is a signaling component of plant systemic acquired resistance (SAR) and induces plant resistance through the NO-ROS-AzA-G3P signaling pathway. There are few studies on the mechanism of action of azelaic acid in plant growth regulation, and there are no reports in the prior art on azelaic acid promoting plant seed germination and / or plant growth. Summary of the invention

[0005] The purpose of the present invention is to provide a new use of azelaic acid, specifically the use of azelaic acid in promoting plant seed germination and / or plant growth, which can effectively promote the germination of plant seeds and promote the growth of hypocotyls and roots of plant seedlings.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The present invention provides the use of azelaic acid in promoting plant seed germination and / or plant growth.

[0008] Preferably, the soil used for plant seed germination and / or plant growth is saline soil and / or ordinary soil.

[0009] Preferably, the salt concentration in the saline soil is 120-200 mM.

[0010] Preferably, the plant seed germination includes seed germination rate and / or seed germination speed.

[0011] Preferably, the plant growth includes the growth of the hypocotyl and / or the growth of the roots of the plant seedlings.

[0012] Preferably, the concentration of azelaic acid is 0.1-1 mM.

[0013] Preferably, the concentration of azelaic acid is 0.4-0.6 mM.

[0014] Preferably, the plants include Malvaceae plants and / or Poaceae plants.

[0015] The present invention provides a method for promoting plant seed germination and / or plant growth, comprising the following steps: treating plant seeds with a 0.1-1.0 mM azelaic acid solution and then sowing.

[0016] Preferably, the treatment time is 12 to 18 hours.

[0017] Beneficial effects:

[0018] The present invention provides the use of azelaic acid in promoting plant seed germination and / or plant growth. The experiment of the present invention uses azelaic acid solution to treat plant seeds. The results show that azelaic acid is not only beneficial to improving the germination rate of plant seeds, but also can shorten the seed germination time, and the effect is more obvious 1 to 3 days after sowing. At the same time, the treatment with azelaic acid is also beneficial to the growth of the hypocotyl and roots of plant seedlings. It can be seen that the azelaic acid has obvious advantages in plant seed germination and / or plant growth.

[0019] At the same time, the present invention also specifically defines the soil used for plant seed germination and / or plant growth as saline soil and / or ordinary soil. The present invention simulates ordinary soil and saline soil by using filter paper soaked in salt solutions of different concentrations. The results show that compared with the control group, azelaic acid not only promotes plant seed germination and seedling growth under ordinary soil conditions, but also significantly promotes plant seed germination and seedling growth under saline soil conditions. DETAILED DESCRIPTION

[0020] The present invention provides the use of azelaic acid in promoting plant seed germination and / or plant growth.

[0021] In the present invention, the concentration of azelaic acid is preferably 0.1-1 mM, more preferably 0.4-0.6 mM, and most preferably 0.5 mM. The azelaic acid solution is preferably prepared by first dissolving it in ethanol and then preparing it into an aqueous solution of a certain concentration. The molecular formula of azelaic acid is C9H 16 O4, structural formula HOOC(CH2)7COOH, molecular weight 188.22. In the embodiment of the present invention, the azelaic acid was purchased from Merck KGaA.

[0022] In the present invention, the soil used for plant seed germination and / or plant growth is preferably saline soil and / or ordinary soil. In the present invention, the concentration of salt in the saline soil is preferably 120-200 mM, more preferably 140-160 mM, and most preferably 150 mM. The concentration of the ordinary soil is preferably 0-50 mM, more preferably 0-30 mM, and most preferably 0 mM. The salt preferably includes NaCl. In the embodiment of the present invention, the soil environment is simulated by filter paper soaked in NaCl salt solution.

[0023] In the present invention, the plants preferably include Malvaceae plants and / or Gramineae plants. The Malvaceae plants preferably include cotton and / or corn, and the Gramineae plants preferably include wheat and / or rice. In the embodiments of the present invention, cotton is used as a representative of Malvaceae plants, and wheat is used as a representative of Gramineae plants to illustrate the application of azelaic acid in promoting the germination of Malvaceae plants or Gramineae plant seeds, and the application of azelaic acid in promoting the growth of Malvaceae plants and / or Gramineae plants, respectively.

[0024] In the present invention, the plant seed germination preferably includes seed germination rate and / or seed germination speed. The plant growth preferably includes the growth of the hypocotyl and / or the growth of the root of the plant seedling.

[0025] The present invention provides a method for promoting plant seed germination and / or plant growth, comprising the following steps: treating plant seeds with a 0.1-1.0 mM azelaic acid solution and then sowing.

[0026] In the present invention, the treatment time is preferably 12 to 18 hours, more preferably 15 to 16 hours, and most preferably 16 hours. The treatment method is preferably to soak the plant seeds in azelaic acid solution to allow them to absorb water and swell.

[0027] In the present invention, the sowing method is preferably sowing the seeds treated with azelaic acid solution into ordinary soil and / or saline soil. In order to simulate the environment of natural germination of plant seeds under laboratory conditions, the sowing method is preferably wrapping the plant seeds with filter paper. The filter paper is preferably filter paper soaked in a salt solution. The concentration of the salt solution is preferably 0 to 200 mM, more preferably 140 to 160 mM, and most preferably 150 mM.

[0028] In the present invention, the sowing preferably includes seedling growth. The present invention does not impose any particular limitation on the environmental conditions for the seedling growth, and the seedling growth conditions known in the art can be used.

[0029] In an embodiment of the present invention, filter paper soaked with 0mM salt solution simulates ordinary soil, and filter paper soaked with 150mM and 200mM salt solution simulates saline soil environment to study the effects of different concentrations of azelaic acid (0mM, 0.1mM, 0.5mM and 1mM) on the germination rate and germination speed of plant seeds. The experimental results show that when planted in ordinary soil, compared with the control group, azelaic acid can significantly promote the germination rate of plant seeds, and with the increase of azelaic acid concentration, the seed germination rate shows a trend of first increasing and then decreasing. When the concentration of azelaic acid is 0.5mM, the germination rate is the highest, and the seed germination speed is also correspondingly increased.

[0030] The present invention takes cotton as an example, uses filter paper soaked with 0mM salt solution to simulate ordinary soil, and uses filter paper soaked with 150mM and 200mM salt solution to simulate saline soil environment, and studies the effects of different concentrations of azelaic acid (0mM, 0.1mM, 0.5mM and 1mM) on seed germination rate and germination speed. The results show that when the concentration of the salt solution is 0mM, the 0.5mM azelaic acid group can promote seed germination 1 to 5d after sowing, and the effect is significant 1d after sowing, and the 1mM azelaic acid group can promote seed germination 1d after sowing. Therefore, the preferred concentration of azelaic acid in promoting seed germination in ordinary soil is 0.5 to 1mM, more preferably 0.5mM, and the time is preferably 1 to 5d, more preferably 1d.

[0031] When the concentration of the salt solution is 150mM, the 0.1mM azelaic acid group, 0.5mM azelaic acid group and 1mM azelaic acid group can significantly promote seed germination 1 to 5 days after sowing, and the 0.5mM azelaic acid group has the best effect. Therefore, the preferred concentration of azelaic acid for promoting seed germination in 150mM saline soil is 0.1 to 1mM, more preferably 0.5mM, and the time is preferably 1 to 5d, more preferably 1 to 3d. When the concentration of the salt solution is 200mM, the 0.1mM azelaic acid group can significantly promote seed germination 1 day after sowing, and the 0.5mM azelaic acid group and the 1mM azelaic acid group can significantly promote seed germination 1 to 3 days after sowing, and the 0.5mM azelaic acid group has the best effect. Therefore, the preferred concentration of azelaic acid for promoting seed germination in 200mM saline soil is 0.1 to 1mM, more preferably 0.5mM, and the time is preferably 1 to 3 days, more preferably 1 day.

[0032] The present invention further takes wheat as an example, uses filter paper soaked with 0mM salt solution to simulate ordinary soil, and uses filter paper soaked with 150mM and 200mM salt solution to simulate saline soil environment, and studies the effects of different concentrations of azelaic acid (0mM, 0.1mM, 0.5mM and 1mM) on seed germination rate and germination speed. The results show that when the concentration of the salt solution is 0mM, the 0.1mM azelaic acid group and the 0.5mM azelaic acid group can promote seed germination 1 to 5d after sowing, and the effect is significant 1d after sowing, and the 0.5mM azelaic acid group has a better effect on promoting seed germination. Therefore, the preferred concentration of azelaic acid in promoting seed germination in ordinary soil is 0.1 to 0.5mM, more preferably 0.5mM, and the time is preferably 1 to 5d, more preferably 1d. When the salt solution concentration is 150mM, 0.5mM azelaic acid group can promote seed germination 1 to 5 days after sowing, and the effect is significant 1 day after sowing. Therefore, the preferred concentration of azelaic acid to promote seed germination in 150mM saline soil is 0.4 to 0.5mM, more preferably 0.5mM, and the time is preferably 1 to 5 days, more preferably 1 day. When the salt solution concentration is 200mM, azelaic acid cannot effectively promote seed germination.

[0033] The present invention further determines the effect of different concentrations of azelaic acid (0mM, 0.1mM, 0.5mM and 1mM) on the growth of hypocotyls of cotton seedlings. The results show that when the concentration of the salt solution is 0mM, the 0.5mM azelaic acid group and the 1mM azelaic acid group can promote the growth of the hypocotyls of the seedlings 3 to 5 days after sowing, and the effect is significant at 3 days after sowing, and the 1mM azelaic acid group has a better effect. Therefore, the preferred concentration of azelaic acid in promoting the growth of the hypocotyls of seedlings in ordinary soil is 0.5 to 1mM, more preferably 1mM, and the time is preferably 3 to 5d, more preferably 3d. When the concentration of the salt solution is 150mM, the 0.1mM azelaic acid group, the 0.5mM azelaic acid group and the 1mM azelaic acid group can significantly promote the growth of the hypocotyl of the seedlings 3 to 5 days after sowing, and the 1mM azelaic acid group has the best effect. Therefore, the preferred concentration of azelaic acid to promote the growth of the hypocotyl of the seedlings in 150mM saline soil is 0.1 to 1mM, more preferably 1mM, and the time is preferably 3 to 5d. When the concentration of the salt solution is 200mM, the 0.5mM azelaic acid group and the 1mM azelaic acid group can significantly promote the growth of the hypocotyl of the seedlings 3d after sowing, and the 0.5mM azelaic acid group has the best effect. Therefore, the preferred concentration of azelaic acid to promote the growth of the hypocotyl of the seedlings in 200mM saline soil is 0.5 to 1mM, more preferably 0.5mM, and the time is preferably 3d.

[0034] The present invention further determines the effect of different concentrations of azelaic acid (0mM, 0.1mM, 0.5mM and 1mM) on the growth of cotton roots. The results show that when the salt solution concentration is 0mM, the 0.5mM azelaic acid group and the 1mM azelaic acid group can promote the growth of roots 3 to 5 days after sowing, and the 1mM azelaic acid group has a better effect. Therefore, the preferred concentration of azelaic acid to promote root growth in ordinary soil is 0.5 to 1mM, more preferably 1mM, and the time is preferably 3 to 5d. When the salt solution concentration is 150mM, the 0.1mM azelaic acid group, the 0.5mM azelaic acid group and the 1mM azelaic acid group can significantly promote the growth of roots 3 to 5d after sowing, and the 0.5mM azelaic acid group has the best effect. Therefore, the preferred concentration of azelaic acid to promote root growth in 150mM saline soil is 0.1 to 1mM, more preferably 0.5mM, and the time is preferably 3 to 5d. When the concentration of the salt solution is 200mM, the 0.1mM azelaic acid group, 0.5mM azelaic acid group and 1mM azelaic acid group can promote root growth 3 to 5 days after sowing, and the 1mM azelaic acid group has the best effect. Therefore, the preferred concentration of azelaic acid to promote root growth in 200mM saline soil is 0.1 to 1mM, more preferably 1mM, and the time is preferably 3 to 5 days.

[0035] In order to further illustrate the present invention, the technical solutions provided by the present invention are described in detail below in conjunction with embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0036] Example 1

[0037] After dissolving azelaic acid in ethanol, it was prepared into aqueous solutions of different concentrations. Cotton seeds were soaked in azelaic acid solutions of different concentrations (0mM, 0.1mM, 0.5mM and 1mM) overnight to absorb water and swell for 16 hours. Then, the seeds were wrapped with filter paper soaked with different concentrations of NaCl salt solutions (0mM, 150mM and 200mM), placed in a flat dish and placed in a plant growth chamber for cultivation. The temperature of the plant growth chamber was set to 28±2℃ and the humidity was 40%, and dark cultivation was performed. The variety of cotton tested was Lumianyan No. 37.

[0038] The seed germination rates were measured 1d, 3d and 5d after sowing. The results are shown in Table 1.

[0039] The sowing experiment found that if cotton seeds have not germinated within 5 days after sowing, they basically will not germinate again and are seeds without vitality. Therefore, only the germination rate 5 days after sowing was counted.

[0040] Table 1 Effect of azelaic acid treatment on cotton seed germination rate (%)

[0041]

[0042] The results showed that low concentrations of azelaic acid (0.1mM, 0.5mM) were more conducive to promoting seed germination, and the effect was more obvious 1 to 3 days after sowing. Azelaic acid can be used to promote seed germination in soils with different salt concentrations, and the best effect was achieved when the salt concentration was 150mM.

[0043] Specifically, when the concentration of the salt solution is 0mM, the 0.5mM azelaic acid group can promote seed germination 1 to 5 days after sowing, and the effect is significant 1 day after sowing, and the 1mM azelaic acid group can promote seed germination 1 day after sowing. When the concentration of the salt solution is 150mM, the 0.1mM azelaic acid group, the 0.5mM azelaic acid group and the 1mM azelaic acid group can significantly promote seed germination 1 to 5 days after sowing, and the 0.5mM azelaic acid group has the best effect. When the concentration of the salt solution is 200mM, the 0.1mM azelaic acid group can significantly promote seed germination 1 day after sowing, the 0.5mM azelaic acid group and the 1mM azelaic acid group can significantly promote seed germination 1 to 3 days after sowing, and the 0.5mM azelaic acid group has the best effect.

[0044] The length of the hypocotyl of cotton seedlings was measured at 3d and 5d after sowing. The results are shown in Table 2.

[0045] Table 2 Effect of azelaic acid on the growth of cotton seedling hypocotyls (cm)

[0046]

[0047] When the concentration of the salt solution was 0mM, the 0.5mM azelaic acid group and the 1mM azelaic acid group could promote the growth of the hypocotyl of the seedlings 3-5 days after sowing, and the effect was significant at 3 days after sowing, and the 1mM azelaic acid group had a better effect. When the concentration of the salt solution was 150mM, the 0.1mM azelaic acid group, the 0.5mM azelaic acid group and the 1mM azelaic acid group could significantly promote the growth of the hypocotyl of the seedlings 3-5 days after sowing, and the 1mM azelaic acid group had the best effect. When the concentration of the salt solution was 200mM, the 0.5mM azelaic acid group and the 1mM azelaic acid group could significantly promote the growth of the hypocotyl of the seedlings 3 days after sowing, and the 0.5mM azelaic acid group had the best effect.

[0048] The length of cotton roots was measured at 3d and 5d after sowing. The results are shown in Table 3.

[0049] Table 3 Effect of azelaic acid on cotton root growth (cm)

[0050]

[0051] The results showed that when the concentration of the salt solution was 0mM, the 0.5mM azelaic acid group and the 1mM azelaic acid group could promote root growth 3-5 days after sowing, and the 1mM azelaic acid group had a better effect. When the concentration of the salt solution was 150mM, the 0.1mM azelaic acid group, the 0.5mM azelaic acid group and the 1mM azelaic acid group could significantly promote root growth 3-5 days after sowing, and the 0.5mM azelaic acid group had the best effect. When the concentration of the salt solution was 200mM, the 0.1mM azelaic acid group, the 0.5mM azelaic acid group and the 1mM azelaic acid group could promote root growth 3-5 days after sowing, and the 1mM azelaic acid group had the best effect.

[0052] According to the data in Tables 1 to 3, the effect of azelaic acid on seeds is dose-dependent. Compared with the control (water), low concentration (0.1mM, 0.5mM) azelaic acid treatment can increase the germination rate, hypocotyl length and root length of cotton seeds under salt stress; high concentration (1.0mM) treatment will affect seed germination, but will still promote the elongation of the hypocotyl and root.

[0053] Example 2

[0054] The only difference between Example 2 and Example 1 is that the seeds tested are wheat, and the variety of wheat tested is Jimai 22. Other operations are the same as in Example 1.

[0055] The seed germination rates were measured 1d, 3d and 5d after sowing. The results are shown in Table 4.

[0056] Table 4 Germination rate of wheat seeds after treatment with different concentrations of azelaic acid (%)

[0057]

[0058] The results showed that when the salt solution concentration was 0mM, the 0.1mM azelaic acid group and the 0.5mM azelaic acid group could promote seed germination 1 to 5 days after sowing, and the effect was significant 1 day after sowing, and the 0.5mM azelaic acid group had a better effect on promoting seed germination. When the salt solution concentration was 150mM, the 0.5mM azelaic acid group could promote seed germination 1 to 5 days after sowing, and the effect was significant 1 day after sowing. When the salt solution concentration was 200mM, azelaic acid could not effectively promote seed germination.

[0059] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. Application of azelaic acid in promoting plant seed germination and / or plant growth.

2. The use according to claim 1, characterized in that: The soil used for plant seed germination and / or plant growth is saline soil and / or normal soil.

3. The application according to claim 2, characterized in that: The salt concentration in the saline soil is 120-200 mM.

4. The use according to claim 1, characterized in that: The plant seed germination includes seed germination rate and / or seed germination speed.

5. The use according to claim 1, characterized in that: The plant growth includes the growth of the hypocotyls of the plant seedlings and / or the growth of the roots.

6. The use according to claim 1, characterized in that: The concentration of the azelaic acid is 0.1-1 mM.

7. The use according to claim 6, characterized in that: The concentration of the azelaic acid is 0.4-0.6 mM.

8. The use according to any one of claims 1 to 7, characterized in that: The plants include plants of the Malvaceae family and / or plants of the Poaceae family.

9. A method for promoting plant seed germination and / or plant growth, characterized in that: The following steps are involved: Plant seeds are treated with a 0.1-1.0 mM azelaic acid solution before sowing.

10. The method according to claim 9, characterized in that: The treatment time is 12 to 18 hours.

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