Application of 2-cyano-3-amino acrylate compound in prevention and treatment of fungal diseases caused by pestalotiopsis

Abstract

The invention discloses an application of a 2-cyano-3-amino acrylate compound in prevention and treatment of fungal diseases caused by pestalotiopsis, and the compound has general ultrahigh bacteriostatic activity on pestalotiopsis fungi in vitro, including pestalotiopsis, pestalotiopsis neospecies and pestalotiopsis pseudospecies. Wherein the effective medium concentration (EC50) range of the phenamacril to 56 pestalotiopsis-like pathogenic bacteria picked from tea trees, peach trees and grapes is 0.1455-0.6025 [mu] g / mL, the average value is 0.2800 [mu] g / mL, and the standard deviation is 0.095 [mu] g / mL. According to the scheme of spraying the JS399-19 suspending agent to treat the tea trees, the tea yield can be remarkably increased, and a basis is provided for preventing and treating diseases caused by pestalotiopsis, new pestalotiopsis and pseudopestalotiopsis in a tea garden and monitoring drug resistance.

Description

Technical Field

[0001] The present invention relates to the technical field of agricultural prevention and control, and specifically to the application of 2-cyano-3-aminoacrylate compounds in preventing and controlling fungal diseases caused by Pseudomonas aeruginosa. Background Art

[0002] Pestalotiopsis-like fungi, including Pestalotiopsis, Neopestalotiopsis and Pseudopestalotiopsis, are asexual fungi in the Amphisphaeriaceae family, with conidia bearing appendages. This fungal group is widely distributed worldwide, with particularly rich biodiversity in tropical and subtropical ecosystems. It has low host specificity and can cause a variety of diseases in a variety of plants, making it an important plant pathogen. Neopestalotiopsis-like fungi are important pathogens that harm the growth of crops such as tea trees and fruit trees. For example, Neopestalotiopsis, which causes tea tree ring spot disease, can cause strawberry red leaf root rot, macadamia nut dry flower disease, betel nut leaf spot, guava fruit ring spot, grape tip dieback, blueberry branch dieback, rubber tree Neopestalotiopsis leaf spot, bean leaf spot, etc. Neopestalotiopsis can survive for many years in infected crop debris and soil, and when the environmental conditions reach a temperature of about 35°C and the relative humidity is >85%, it is conducive to the development of the disease.

[0003] The cultivation of fruit and tea such as grapes, pears, peaches, apples, and tea usually forms perennial orchards, which undergo countless agronomic operations such as pruning during their life cycle. The fungi of the class of Pseudomonas aeruginosa mainly invade through natural wounds caused by agricultural operations such as pruning, and some pathogens can also invade through new shoots or / and tender leaves. At present, the prevention and control of this type of disease is still mainly based on spraying chemical pesticides and supplemented by biological pesticides. Commonly used fungicides are mainly demethylation inhibitors (tebuconazole, fenpropimorph and myclobutanil, etc.) and benzimidazole fungicides (including benomyl, thiophanate and carbendazim, etc.). With the long-term single use of drugs, pathogens develop drug resistance, the efficacy of the drugs decreases year by year, and even completely fails. Therefore, it is necessary to find low-toxic and low-residue pesticides with different modes of action, which can be used in rotation or in combination with currently commonly used fungicides to reduce the development of drug resistance.

[0004] 2-Cyano-3-amino-3-phenyl acrylate compounds are a new type of fungicide with a novel structure and unique mode of action. Cyanocyanobacterium has been successfully industrialized. Studies have shown that cyanocyanobacterium is a slightly toxic fungicide. Its toxicity to four environmental organisms, such as bees, quails, silkworms, and fish, is considered to be low to moderate, and it has good environmental compatibility. Cyanocyanobacterium is a highly specific and selective fungicide that mainly exhibits antibacterial activity against Fusarium, especially against the mycelial growth of Fusarium graminearum and Fusarium moniliforme. EC 50 The values ​​were 0.141μg / mL and 0.459μg / mL respectively, and the relative toxicity was 4.06 and 1.43 times that of carbendazim, and there was no cross-resistance with existing fungicides. At present, there is no clear report on whether cyanothiocarb has an antibacterial effect on the fungus of Pseudomonas aeruginosa in fruit and tea crops. Summary of the invention

[0005] Purpose of the invention: In view of the problem that existing pesticide fungicides gradually develop resistance to Pseudomonas-like fungal strains, the present invention provides a new fungicide for use in preventing and controlling Pseudomonas-like fungi. The present invention provides the use of 2-cyano-3-aminoacrylate compounds in preventing and controlling fungal diseases caused by Pseudomonas-like fungi. It is the first time that 2-cyano-3-aminoacrylate compounds are found to be significantly effective in preventing and controlling Pseudomonas-like fungal diseases.

[0006] The invention also provides the bactericidal composition for preventing and controlling the fungal diseases of Pseudomonas spp.

[0007] Technical solution: In order to achieve the above-mentioned purpose, the 2-cyano-3-aminoacrylate compound as described in Formula I of the present invention is used in preventing and treating fungal diseases caused by Pseudodiscotrichum, wherein the Pseudodiscotrichum includes Pseudodiscotrichum, Pseudodiscotrichum and Neodiscotrichum fungi, and the structure of the 2-cyano-3-aminoacrylate compound is as shown in Formula I:

[0008]

[0009] Wherein: R1 is hydrogen, amino, substituted amino, cyano, nitro, phenyl, halophenyl, halogen, C1-C6 alkyl, aromatic heterocyclic group, hydroxyl or ester group;

[0010] R2 is a C1-C6 alkyl group or a C3-C6 cycloalkyl group.

[0011] Wherein, the substituted amino group is optionally substituted by 0 to 2 substituents independently selected from -OH, halogen, -CN or NO2, or is a primary, secondary or tertiary substituted amino group; the aromatic heterocyclic group is a 5-10 membered heteroaryl group containing 1 to 4 heteroatoms independently selected from N, O or S; the halogen is fluorine, chlorine, bromine or iodine.

[0012] Wherein, the 2-cyano-3-aminoacrylate compound is selected from any one of the following compounds:

[0013]

[0014]

[0015] The present invention discloses an application of the 2-cyano-3-aminoacrylate compound of formula I in the preparation of a drug or reagent for preventing and treating diseases caused by fungal pathogens of the genera Trichodesmium, Pseudotrichodesmium and Neotrichodesmium.

[0016] Among them, the 2-cyano-3-aminoacrylate compounds can prevent and control diseases such as gray spot and ring spot caused by Pseudomonas-like fungi, as well as diseases caused by artificial wounds caused by horticultural measures such as pruning or trimming of fruit trees or tea trees.

[0017] The bactericidal composition for preventing and controlling the fungal diseases of the Pseudomonas spp. of the present invention comprises a 2-cyano-3-aminoacrylate compound as an active ingredient and is added with a pesticide adjuvant or auxiliary material.

[0018] The bactericidal composition for preventing and controlling the fungal diseases of the Pseudomonas spp. of the present invention comprises the 2-cyano-3-aminoacrylate compound as one of the active ingredients, combined with other effective bactericidal ingredients, and added with pesticide adjuvants or auxiliary materials to form a compound preparation.

[0019] Wherein, the composition is emulsifiable concentrate, suspension concentrate, wettable powder, water dispersible granules, aqueous emulsion or microemulsion.

[0020] The 2-cyano-3-aminoacrylate compound of the present invention can be used for the prevention and treatment of the fungal diseases of the same type as the Pseudomonas aeruginosa in the form of a single-dose preparation. The 2-cyano-3-aminoacrylate compound is used as an active ingredient, and a pesticide adjuvant is added to prepare an emulsifiable concentrate, a suspension, a wettable powder, a water-dispersible granule, an aqueous emulsion, a microemulsion, a seed treatment agent and the like. The present invention can also be used for the prevention and treatment of the fungal diseases of the same type as the Pseudomonas aeruginosa in the form of a bactericidal composition, which uses the 2-cyano-3-aminoacrylate compound as one of the bactericidal active ingredients, is combined with other bactericidal effective ingredients, and a pesticide adjuvant is added to prepare a compound preparation, which plays a role in enhancing synergy or expanding the scope of application.

[0021] The invention discloses an application of the fungicidal composition for preventing and controlling Pseudomonas-like fungal diseases in preventing and controlling Pseudomonas-like fungal diseases.

[0022] The sterilization method for preventing and controlling the fungal pathogen of the Pseudomonas aeruginosa of the present invention comprises spraying a liquid medicine containing the 2-cyano-3-aminoacrylate compound as an active ingredient after pruning trees in the field, or spraying the sterilization composition for preventing and controlling the fungal disease of the Pseudomonas aeruginosa.

[0023] The field trees refer to fruit trees (such as peaches, pears, grapes, apples, etc.) and tea trees that require artificial pruning or gardening measures to cause injuries.

[0024] The present invention provides a method for determining the toxicity of a fungicide of the type Pseudomonas spp. and its application, comprising the following steps:

[0025] (1) Cultivate the test pathogens;

[0026] The tested pathogens are monosporic fungal strains of Pseudodiscotrichum spp. isolated and purified from diseased branches of tea trees, peach trees and grapes.

[0027] The sample separation and purification is as follows: the sample is cut into 5 mm small pieces at the junction of the diseased and healthy parts, disinfected with 2% sodium hypochlorite for 5 seconds in a sterile operating table, rinsed with sterile water 3 times, dried with absorbent paper and transferred to a potato culture medium (PDA) containing 100 μg / mL streptomycin sulfate, and cultured in a 25°C incubator for 3 days. After that, the mycelium blocks of each strain were transferred to a new PDA culture medium. After culturing in the dark at 25°C for 14 days, the colonies were rinsed with 5 mL of distilled water to collect conidia and prepared into 1×10 4 10 μl of the spore suspension was evenly spread on a PDA plate containing 100 μg / mL streptomycin sulfate, and after culturing in a 25°C incubator for 1 day, single spores were picked out and transferred to fresh PDA. Finally, single spore colonies of the Pseudomonas aeruginosa were obtained, which were transferred to cryopreservation tubes and stored at 4°C for later use.

[0028] The potato culture medium (PDA) comprises 200 g potato, 15 g agar powder, and 20 g glucose, which is diluted to 1 L with pure water, sterilized at high temperature and high pressure, and then kept at room temperature for use.

[0029] (2) Preparation of pesticides and fungicides

[0030] The bactericide is cyanothiocarb, and the original drug of 2-cyano-3-aminoacrylate compound is prepared with methanol to 1×10 4 μg / mL stock solution for future use.

[0031] (3) Determination of the effective median concentration (EC) of 2-cyano-3-aminoacrylate compounds against Pseudomonas spp. in tea, peach and grape 50 )

[0032] Use a 5 mm puncher to punch a bacterial plate at the edge of the test strain colony. Evenly mix the drug mother solution with the culture medium to obtain final concentrations of 0, 0.025, 0.05, 0.1, 0.2, 0.4, 0.8, and 1.6 μg / mL, respectively. Pour the mixed solution into a 90 mm diameter culture dish to make a plate. Place the bacterial plate in the center of a PDA plate containing a series of concentrations of 2-cyano-3-aminoacrylate compounds, and inoculate one bacterial plate per dish. Cultivate at 25°C in the dark until the control colony covers 60% of the culture dish, and measure the colony diameter using the cross method. Repeat each treatment 3 times. Calculate the growth inhibition rate from the average colony diameter (mm).

[0033] Among them, mycelium growth inhibition rate (%) = (control colony diameter - each treatment colony diameter) / (control colony diameter - 5) × 100%

[0034] (4) Collect data on the effective median concentration of 2-cyano-3-aminoacrylate compounds for the test strains and draw a sensitivity curve.

[0035] Among them, in the step of statistically analyzing the effective medium concentration data of 2-cyano-3-aminoacrylate compounds on the test strains and drawing the sensitivity curve: the toxicity regression equation Y = a + bX was calculated using DPS software, and the effective inhibition medium concentration (EC 50 ), correlation coefficient (r) and 95% confidence interval. Calculate the EC of the test strain 50 Mean and standard deviation, according to EC 50 Distribution frequency, plot sensitivity baseline.

[0036] (5) In a newly pruned tea garden, randomly select a plot and prepare a 25% suspension with the agent at 200 ml / mu and 250 L / hm2. 2 Add water and spray with a sprayer when there is no wind, with clean water as the control. Each treatment is repeated 3 times, and the second spray is carried out after 10 days. After 6 weeks, the five-point sampling method is used to record the weight of 100 buds and the number of buds in each plot (33×33cm).

[0037] The 2-cyano-3-aminoacrylate compounds of the present invention exhibited a general ultra-high antibacterial activity against Pseudodiscotrichum-like fungi in vitro, including Pseudodiscotrichum, Neodiscotrichum and Pseudodiscotrichum. Among them, the effective medium concentration (EC) of cyanobacterium methyl against 56 strains of Pseudodiscotrichum pathogens collected from tea trees, peach trees and grapes was 50) ranged from 0.1455 to 0.6025 μg / mL, with an average value of 0.2800 μg / mL and a standard deviation of 0.095 μg / mL. The scheme of spraying tea trees with cyanobacterium suspension provided by the present invention can significantly increase tea yield, and provide a basis for controlling diseases caused by pathogens of Pseudomonas aeruginosa, Neo-Pseudomonas aeruginosa and Pseudomonas aeruginosa in tea gardens, as well as for drug resistance monitoring.

[0038] Beneficial effects: Compared with the prior art, the present invention has the following advantages:

[0039] (1) The fungicide 2-cyano-3-aminoacrylate compound of the present invention has a novel mechanism of action and has excellent inhibitory activity against the fungus of the type Pseudomonas aeruginosa. It is proposed for the first time that it can be used for the prevention and treatment of the fungus of the type Pseudomonas aeruginosa, thereby improving the effect of preventing and treating diseases including wheel spot disease and gray spot disease.

[0040] (2) The sensitivity baseline of the fungus pathogen of the pseudodiscus polysporus provided by the present invention to cyanothiocarb, and the EC value of the fungus pathogen of the pseudodiscus polysporus to cyanothiocarb. 50 The average value was 0.2800 μg / mL, and the standard deviation was 0.095 μg / mL. This provides a scientific basis for the field control of Trichosporon fungi with cyanocobalamin. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 This is the frequency distribution diagram of the sensitivity of 56 strains of Pseudomonas aeruginosa fungi to cyanothiocarb;

[0042] Figure 2 This is a comparison chart of the inhibition of cyanothiocarb on the hyphae growth of Pseudodiscotrichum fungi;

[0043] Figure 3 This is a comparison chart of cyanocbiconamide in field control of Trichosporon-like fungi. DETAILED DESCRIPTION

[0044] The technical solution of the present invention is described clearly and completely below in conjunction with the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in the field without creative work are within the scope of protection of the present invention.

[0045] The experimental methods described in the examples are conventional methods unless otherwise specified; the reagents and materials described are commercially available unless otherwise specified.

[0046] The various pathogenic bacteria used in the present invention, such as MJ-1, MJ-5, MJ-7, MJ-127, and MJ-13, are all wild-type fungal strains of the pseudodiscopolysporum fungus, which are collected from diseased branches of tea, grapes, and peaches, and the pathogenic bacteria are separated and purified. The pathogenic bacteria in the present invention can also be obtained by purchase. The pathogenic bacteria used in the present invention are all provided by Jiangsu Pesticide Research Institute Co., Ltd.

[0047] MJ-1 was identified as Pestalotiopsis; MJ-5, MJ-7, and MJ-127 were identified as Neopestalotiopsis; and MJ-13 was identified as Pseudopestalotiopsis. Specifically, MJ-1, MJ-5, MJ-7, MJ-127, and MJ-13 are Pestalotiopsis camelliae, N. clavispora, N. ellipsospora, N. asiatica, and Pseudopestalotiopsis cocos, respectively.

[0048] The compounds cyanoacrylate (cas: 3336-69-4), ZJS158 (cas: 2810129-50-9), ZJS159 (cas: 2810129-52-1), and ZJS178 (cas: 2810129-51-0) in the present invention are all known compounds.

[0049] The synthesis of ZJS232 and ZJS288 can be performed with reference to CN101417962A, as well as with reference to the known synthesis methods of ZJS158, ZJS159 and ZJS178.

[0050] Example 1

[0051] Screening of the activity of cyanoacrylate fungicides

[0052] Test subjects: Pseudomonas pseudodiscus strains MJ-1, MJ-5, MJ-7, MJ-127, and MJ-13. Diseased branches were collected from tea gardens and the pathogen was isolated and purified.

[0053] The test pathogen was a monosporic strain isolated and purified from diseased branches of tea trees, grapes and peaches collected from different tea gardens in Nanjing, Jiangsu Province in 2022.

[0054] The sample was isolated and purified as follows: the diseased branch sample was cut into 5 mm small pieces at the junction of the diseased and healthy branches, disinfected with 2% sodium hypochlorite for 5 seconds in a sterile operating table, rinsed with sterile water 3 times, dried with absorbent paper and transferred to potato culture medium (PDA) containing 100 μg / mL streptomycin sulfate. After culturing in a 25°C incubator for 3 days, the mycelial blocks of each strain were transferred to new PDA culture medium. After culturing in the dark at 25°C for 14 days, the colonies were rinsed with 5 mL of distilled water to collect conidia and prepared into 1×10 4 A spore suspension of 100 μL of conidia / mL was added with 1% Tween 20, and 10 μL of the spore suspension was evenly spread on a PDA plate containing 100 μg / mL streptomycin sulfate. After culturing in a 25°C incubator for 1 day, single spores were picked out and the single colonies were transferred to fresh PDA. Finally, single spore colonies of various types of Trichosporon fungi were obtained, which were named MJ-1, MJ-5, MJ-7, MJ-127, and MJ-13, respectively, and transferred to cryopreservation tubes for storage at 4°C for later use.

[0055] Use a 5mm puncher to punch a bacterial plate at the edge of the test strain colony in the logarithmic phase, mix the drug mother solution with the culture medium evenly, set the drug concentration to 0, 0.0625, 0.025, 0.05, 0.1, 0.2, 0.25, 0.4, 0.8, 1, 1.6μg / mL, pour the mixed solution into a 90mm diameter culture dish to make a plate, place the bacterial plate in the center of the PDA plate containing a series of concentrations of the test drug, and inoculate one bacterial plate per dish. Cultivate at 25℃ in the dark until the control colony covers 60% of the culture dish, and measure the colony diameter using the cross method. Repeat each treatment 3 times. Calculate the growth inhibition rate from the average colony diameter (mm).

[0056] Among them, mycelium growth inhibition rate (%) = (control colony diameter - each treatment colony diameter) / (control colony diameter - 5) × 100%. The test results are as follows, as shown in Table 1 below.

[0057] Table 1 Inhibitory activity of different compounds

[0058]

[0059]

[0060] The test results show that the various 2-cyano-3-aminoacrylate compounds in Table 1 exhibit certain antibacterial activity against the fungi of the class of Pseudomonas spp. at relatively low concentrations. In particular, the inhibition rate of compound ZJS178 against the fungi of the class of Pseudomonas spp. can reach more than 90%. The test compounds of the present invention all exhibit super high activity against the fungi of the class of Pseudomonas spp.

[0061] Example 2

[0062] Toxicity test of cyproconazole against 56 pathogenic fungi of Pseudomonas spp.

[0063] Use a 5mm puncher to punch a bacterial plate at the edge of the colony of the test strain that has been cultured to the logarithmic phase, mix the drug mother solution with the culture medium evenly, set the drug concentration to 0, 0.025, 0.05, 0.1, 0.2, 0.4, 0.8, 1.6μg / mL, pour the mixed solution into a 90mm diameter culture dish to make a plate, place the bacterial plate in the center of the PDA plate containing a series of concentrations of cyproconazole, and inoculate one bacterial plate per dish. Cultivate at 25℃ in the dark until the control colony covers 60% of the culture dish, and measure the colony diameter using the cross method. Repeat each treatment 3 times. Calculate the growth inhibition rate from the average colony diameter (mm), such as Figure 2 shown.

[0064] The mycelium growth inhibition rate (%) = (control colony diameter - each treatment colony diameter) / (control colony diameter - 5) × 100%.

[0065] The effective median concentration data of cyanocobalamin technical for the test strains were collected and the sensitivity curve was drawn.

[0066] Among them, in the step of statistically analyzing the effective medium concentration data of cyanobacterium ester on the test strains and drawing the sensitivity curve: the toxicity regression equation Y = a + bx was calculated using DPS software, and the effective inhibition medium concentration (EC 50 ), correlation coefficient (r). Calculate the EC of the test strain 50 Mean and standard deviation, according to EC 50 Distribution frequency, plot sensitivity baseline.

[0067] Table 2 Toxicity of cyanobacterium to 56 strains of Pseudomonas spp.

[0068]

[0069]

[0070] The sensitivity of 56 monospore strains of Pestalotiopsis sp. and Neopestalotiopsis sp. collected and isolated from Jiangsu in 2022 to cyanothiocarb was determined by mycelial growth rate assay. 50 The value was 0.1455~0.6025μg / mL, and the average EC 50 The value was 0.2800±0.0952μg / mL, and the standard deviation was 0.095μg / mL. The frequencies of strains with different sensitivities showed a continuous single-peak curve distribution as shown in Tables 2 and Figure 1 It is further proved that the compound of the present invention has excellent antibacterial effect on the fungal pathogen of Pseudomonas aeruginosa.

[0071] Example 3

[0072] Field efficacy test

[0073] The Pseudomonas-like fungi can cause the buds of tea trees to be significantly smaller and their number is significantly reduced. This example further demonstrates the effect of the compounds of the present invention on controlling the Pseudomonas-like fungi through field efficacy determination.

[0074] (1) Experimental subjects: tea trees in tea gardens in Jiangning District, Nanjing City, Jiangsu Province.

[0075] (2) Chemical treatment

[0076] In the newly pruned tea garden, randomly selected plots were treated with (1) 25% cyproconazole SC suspension at 200 ml / mu and 250 L / hm2. 2 Add water, (2) 80% carbendazim WP at 100 g / mu, at 250 L / hm 2 (3) Clean water control. Spray with a sprayer when there is no wind, with 3 replicates for each treatment. The first spray was conducted on June 11, 2023; the second spray was conducted on June 20, 2023. Six weeks after the second spray, the five-point sampling method was used to record the 100 bud weight and the number of buds (33×33cm) in each plot and investigate the control effect.

[0077] Hundred bud weight refers to the total weight of 100 new shoots picked, which is used to measure the quality and thickness of new shoots. The quality of new shoots can be judged by the value of its 100 bud weight. The calculation method of 100 bud weight is to weigh 100 randomly selected new shoots or mixed new shoots with the same number of leaves, and record the average value of more than three times. The results are shown in Table 3.

[0078] Table 3 Effect of cyanocobalamin treatment on tea yield

[0079]

[0080] The five-point sampling method was used to measure the 100-bud weight and number of buds of tea trees in the tea garden of Jiangning District, Nanjing City, Jiangsu Province (33×33cm). The average 100-bud weight of tea trees treated with 25% cyproconazole SC suspension at 200 ml / mu was 10.05 g, which was 8.05 g higher than the average 100-bud weight of the water control. At the same time, the average number of buds of tea trees treated with 25% cyproconazole SC suspension was 29.8±4.20, which was significantly higher than the average number of buds of the water control of 13±1.58 (Table 3, Figure 3 ), which were significantly higher than the average 100 bud weight of 8.81 g and the number of buds of 18.2 in the 80% carbendazim treatment.

Claims

1. Use of the 2-cyano-3-aminoacrylate compound as described in formula I in preventing and treating fungal diseases caused by Pseudomonas spp., wherein the Pseudomonas spp. include Pseudomonas spp., Pseudomonas spp. and Neomorphonas spp. fungi, and the structure of the 2-cyano-3-aminoacrylate compound is as shown in formula I: in: R1 is hydrogen, amino, substituted amino, cyano, nitro, phenyl, halophenyl, halogen, C1-C6 alkyl, aromatic heterocyclic group, hydroxyl or ester group; R2 is a C1-C6 alkyl group or a C3-C6 cycloalkyl group.

2. The use according to claim 1, characterized in that: The substituted amino group is optionally substituted by 0 to 2 substituents independently selected from -OH, halogen, -CN or NO2, or is a primary, secondary or tertiary substituted amino group; the aromatic heterocyclic group is a 5-10 membered heteroaryl group containing 1 to 4 heteroatoms independently selected from N, O or S; the halogen is fluorine, chlorine, bromine or iodine.

3. The use according to claim 1, characterized in that: The 2-cyano-3-aminoacrylate compound is preferably selected from any one of the following compounds:

4. Use of the 2-cyano-3-aminoacrylate compound of formula I in the preparation of a drug or reagent for preventing and treating diseases caused by fungal pathogens of the genera Trichodesmium, Pseudotrichodesmium and Neotrichodesmium.

5. The use according to any of claims 1 to 4, characterized in that: The 2-cyano-3-aminoacrylate compounds can control diseases such as gray spot and ring spot caused by the fungus Pseudomonas spp., and can also control diseases caused by wounds caused by pruning or trimming of fruit trees or tea trees.

6. A fungicidal composition for treating diseases caused by Trichosporon spp., characterized in that: The method comprises the 2-cyano-3-aminoacrylate compound as claimed in claim 1 as an active ingredient, and is added with pesticide adjuvants or auxiliary materials.

7. A fungicidal composition for preventing and treating diseases of the fungus Pseudomonas spp., characterized in that: The compound preparation comprises the 2-cyano-3-aminoacrylate compound as claimed in claim 1 as one of the active ingredients, combined with other effective bactericidal ingredients, and added with pesticide adjuvants or auxiliary materials to prepare a compound preparation.

8. The bactericidal composition according to claim 6 or 7, characterized in that: The composition is emulsifiable concentrate, suspension, wettable powder, water-dispersible granule, aqueous emulsion or microemulsion.

9. A sterilization method for preventing and treating the fungal pathogen of Pseudomonas aeruginosa, characterized in that: After pruning trees in the field, spray the liquid containing the 2-cyano-3-aminoacrylate compound of claim 1 as an active ingredient, or spray the fungicide composition of claim 5 or 6.

10. The sterilization method according to claim 9, characterized in that: The field trees refer to fruit trees or tea trees that require artificial pruning or gardening measures to cause injuries.

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