Composition for improving eggshell quality and application thereof

Abstract

The invention discloses a composition for improving eggshell quality and application thereof, and belongs to the field of feed. The composition comprises the following raw materials in parts by weight: 15-70 parts of a first component and 14-60 parts of a second component, the first component is at least one of ursolic acid and ursolic acid derivatives; and the second component is at least one of quercetagetin and a quercetagetin derivative. The research finds that the combined use of ursolic acid and quercetagetin plays a synergistic role in improving the egg quality of laying hens, and the proportion of dark-fringe eggs produced by the laying hens is greatly reduced. The composition provided by the invention is purely natural, safe and non-toxic, does not cause the problems of toxic and side effects on animals and the like, is beneficial to guaranteeing the safety of animal products, can be used as a safe, efficient, stable and controllable green and environment-friendly feed additive, and has a very good application prospect in the aspect of reducing the proportion of dark-fringe eggs produced by laying hens.

Description

Technical Field

[0001] The invention belongs to the field of feed, and particularly relates to a composition for improving eggshell quality and application thereof. Background Art

[0002] In modern animal husbandry, egg quality is one of the focuses of consumers. Dark-striped eggs (i.e. irregular stripes on the surface of the yolk) will affect the appearance of eggs and consumers' willingness to buy.

[0003] Products that can solve the problem of dark-striped eggs on the market include feed additives and biological preparations. Many feed manufacturers have introduced feed additives containing organic trace elements (such as zinc, manganese, copper), rare earth elements and serine; biological preparations such as bacteriophage preparations can improve eggshell quality and reduce the production of dark-striped eggs by inhibiting or killing microorganisms that cause diseases such as salpingitis.

[0004] If these products are used improperly during their use, they may lead to nutritional imbalance and affect the overall health of laying hens. They are also expensive, increasing breeding costs. The effects of biological agents may be affected by many factors, such as strain adaptability, dosage and timing of use, and long-term use may lead to drug resistance.

[0005] In order to solve the above problems, it is urgent to develop products that improve eggshell quality and fully consider the safety, effectiveness and economy of the products. Summary of the invention

[0006] The object of the present invention is to overcome at least one disadvantage of the prior art and provide a composition for improving eggshell quality and application thereof.

[0007] The technical solution adopted by the present invention is:

[0008] A composition comprising the following raw materials in parts by weight: 15-70 parts of a first component and 14-60 parts of a second component;

[0009] The first component is at least one of ursolic acid and ursolic acid derivatives;

[0010] The second component is at least one of quercetin and a quercetin derivative.

[0011] In some of the embodiments, the composition comprises the following raw materials in parts by weight: 20-65 parts of the first component and 15-40 parts of the second component.

[0012] In some of the embodiments, the composition comprises the following raw materials in parts by weight: 20-60 parts of the first component and 20-40 parts of the second component.

[0013] In some embodiments, the ursolic acid derivative includes ursolic acid acetate, and / or the quercetin derivative includes quercetin-7-O-glucoside and a quercetin fat-soluble derivative.

[0014] Use of the composition described in any one of the above items in any of the following items:

[0015] (i) Use in the preparation of a product for improving eggshell quality;

[0016] (ii) Application in the preparation of feed, medicine, additives, food, health products and cosmetics.

[0017] A product comprising any one of the above compositions.

[0018] In some embodiments, the product includes feed, medicine, additive, food, health care product, and cosmetics.

[0019] In some embodiments, when the product is feed, the content of the composition in the feed is 0.1-0.5 kilograms per ton (kg / t).

[0020] In some embodiments, when the product is an additive, the additive includes a feed additive or a food additive.

[0021] In some embodiments, the dosage form of the product is one of granules, tablets, capsules, powders, pills, suspensions, liquid preparations, and pastes.

[0022] In some embodiments, the method for preparing the composition comprises the following steps:

[0023] (1) Add the first component and the second component in the above proportions into a double-shaft high-efficiency mixer and mix them evenly;

[0024] (2) The mixture is added to a wet granulator for granulation to obtain a granular composition product.

[0025] In some embodiments, the rotation speed of the twin-shaft high-efficiency mixer is 500-1000 r / min.

[0026] In some of the embodiments, when granulation is performed in a wet granulator, the rotation speed of the horizontal stirring blade is 50-100 r / min, and the rotation speed of the vertical stirring blade is 500-1000 r / min, and the time is 10-15 minutes.

[0027] The beneficial effects of the present invention are:

[0028] The present study found that the combined use of ursolic acid and quercetin played a synergistic role in improving egg quality in laying hens, greatly reducing the proportion of dark-striped eggs produced by laying hens.

[0029] The composition provided in the present application is purely natural, safe and non-toxic, will not cause toxic side effects to animals, and is beneficial to ensuring the safety of animal products. It can be used as a safe, efficient, stable, and controllable green and environmentally friendly feed additive, and has good application prospects in reducing the proportion of dark-marked eggs produced by laying hens. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 It is a normal egg shape.

[0031] Figure 2 It is in the form of a dark-patterned egg.

[0032] Figure 3 The results are for egg production rate of each group. DETAILED DESCRIPTION

[0033] The preferred embodiment of the present invention will be described in detail below in conjunction with specific embodiments. It should be noted that the following specific embodiments are provided only for the purpose of illustration and are not intended to limit the scope of the present invention. Those skilled in the art may make various modifications and substitutions to the present invention without departing from the purpose and spirit of the present invention.

[0034] A composition comprising the following raw materials in parts by weight: 15-70 parts of a first component and 14-60 parts of a second component;

[0035] The first component is at least one of ursolic acid and ursolic acid derivatives;

[0036] The second component is at least one of quercetin and a quercetin derivative.

[0037] In some embodiments, the composition comprises the following raw materials in parts by weight: 20-65 parts of the first component and 15-40 parts of the second component.

[0038] In some embodiments, the composition comprises the following raw materials in parts by weight: 20-60 parts of the first component and 20-40 parts of the second component.

[0039] In some embodiments, the ursolic acid derivative includes ursolic acid acetate, and / or the quercetin derivative includes quercetin-7-O-glucoside and a quercetin fat-soluble derivative.

[0040] The use of the above composition in any of the following:

[0041] (i) Use in the preparation of a product for improving eggshell quality;

[0042] (ii) Application in the preparation of feed, medicine, additives, food, health products and cosmetics.

[0043] A product comprising any one of the above compositions.

[0044] In some embodiments, the product includes feed, medicine, additive, food, health care product, and cosmetics.

[0045] In some embodiments, the dosage form of the product is one of granules, tablets, capsules, powders, pills, suspensions, liquid preparations, and pastes.

[0046] In some embodiments, when the product is feed, the content of the composition in the feed is 0.1-0.5 kilograms per ton (kg / t).

[0047] In some preferred embodiments, the feed is a special feed for laying hens, and the content of the composition in the feed is 0.1-0.5 kilograms per ton (kg / t).

[0048] In some embodiments, when the product is an additive, the additive includes a feed additive or a food additive.

[0049] In some preferred embodiments, the feed additive is a feed additive specifically for laying hens.

[0050] In some embodiments, the method for preparing the composition comprises the following steps:

[0051] (1) Add the first component and the second component in the above proportions into a double-shaft high-efficiency mixer and mix them evenly;

[0052] (2) The mixture is added to a wet granulator for granulation to obtain a granular composition product.

[0053] In some embodiments, the rotation speed of the twin-shaft high-efficiency mixer is 500-1000 r / min.

[0054] In some of the embodiments, when granulation is performed in a wet granulator, the rotation speed of the horizontal stirring blade is 50-100 r / min, and the rotation speed of the vertical stirring blade is 500-1000 r / min, and the time is 10-15 minutes.

[0055] The present application provides a safe, effective and economical composition for solving the problem of dark-striped eggs, which contains ursolic acid and quercetin. At the same time, production personnel should strengthen feeding management, improve the overall health of laying hens, and fundamentally reduce the production of dark-striped eggs.

[0056] Ursolic Acid is also known as 3beta-Hydroxyurs-12-en-28-oic acid. Its chemical formula is C 30 H 48 O 3 , the structural formula is CAS No.: 77-52-1, Molecular weight: 456.7g / mol, EINECS No.: 201-034-0. Ursolic acid, also known as ursolic acid and ursolic acid, is a natural triterpenoid compound that is widely used in nature and can be extracted and separated from various medicinal plants and fruits. It has a variety of biological effects such as sedation, anti-inflammatory, antibacterial, anti-diabetic, anti-ulcer, and lowering blood sugar.

[0057] Quercetagetin (QG), also known as 6-Hydroxyquercetin, is 3,3',4',5,6,7-hexahydroxyflavone with molecular formula: C 15 H 10 O 8 , the structural formula is CAS No.: 90-18-6. Quercetin belongs to the flavonol class of compounds and is one of the main components of marigold, a plant of the Asteraceae family. It has a certain antioxidant capacity. The use of antioxidants can largely prevent and eliminate the body's redox balance disorder caused by oxidative stress and improve breeding efficiency. Quercetin is a natural antioxidant extracted from marigold, which has the advantages of safety and high efficiency.

[0058] Through feeding experiments, it can be observed that the production performance of laying hens fed with the composition of the present application is significantly improved, and the eggshell quality is significantly improved, which is specifically manifested in a reduction in the rate of dark-marked eggs.

[0059] In practical applications, the ursolic acid and quercetin composition of the present application can be added to the feed of laying hens as a feed additive. The specific amount of addition can be adjusted according to factors such as the breed of laying hens, the feeding environment, and the feeding stage.

[0060] At the same time, it is recommended to keep the feeding environment clean and sanitary during the feeding process to ensure that the laying hens get enough nutrition and water. In addition, regular health checks on laying hens to promptly detect and deal with potential health problems are also important measures to help improve the production performance of laying hens and the quality of eggs.

[0061] If no specific techniques or conditions are specified in the following examples, the techniques or conditions described in the literature in the field or the product instructions are used. If no manufacturer is specified for the reagents or instruments used, they are all conventional products that can be purchased through regular channels.

[0062] The quercetin (CAS: 90-18-6) and ursolic acid (CAS: 77-52-1) used in the following examples and comparative examples are finished products purchased from Chenguang Biotechnology Group Co., Ltd., wherein the purity of quercetin (CAS: 90-18-6) is 95%-98%, and the purity of ursolic acid (CAS: 77-52-1) is 95%-98%.

[0063] Those skilled in the art may also use quercetin or ursolic acid extracted from various other plants, and when the purity is lower than that of the pure product, convert it into the pure product, and prepare the composition according to the formula of this application. Those skilled in the art should understand that the composition obtained by the adjustment is within the protection scope of this application.

[0064] The following is a description with reference to specific embodiments.

[0065] The model of the double-shaft high-efficiency mixer used in the following examples is SSHJ0.5, with an output of 0.25T / batch and a power of 7.5KW.

[0066] Example 1

[0067] This embodiment provides a composition, comprising the following components in parts by weight: 20 parts of ursolic acid and 20 parts of quercetin.

[0068] Example 2

[0069] This embodiment provides a composition, comprising the following components in parts by weight: 20 parts of ursolic acid and 40 parts of quercetin.

[0070] Example 3

[0071] This embodiment provides a composition, comprising the following components in parts by weight: 60 parts of ursolic acid and 20 parts of quercetin.

[0072] Example 4

[0073] This embodiment provides a composition, comprising the following components in parts by weight: 60 parts of ursolic acid and 40 parts of quercetin.

[0074] Example 5

[0075] This embodiment provides a composition, comprising the following components in parts by weight: 30 parts of ursolic acid and 30 parts of quercetin.

[0076] Example 6

[0077] This embodiment provides a composition, comprising the following components in parts by weight: 65 parts of ursolic acid and 25 parts of quercetin.

[0078] Example 7

[0079] This embodiment provides a composition, comprising the following components in parts by weight: 60 parts of ursolic acid and 24 parts of quercetin.

[0080] Example 8

[0081] This embodiment provides a composition, comprising the following components in parts by weight: 60 parts of ursolic acid and 15 parts of quercetin.

[0082] Comparative Example 1

[0083] This embodiment provides a composition, comprising the following components in parts by weight: 45 parts of ursolic acid and 0 parts of quercetin.

[0084] Comparative Example 2

[0085] This embodiment provides a composition, comprising the following components in parts by weight: 0 parts of ursolic acid and 30 parts of quercetin.

[0086] Comparative Example 3

[0087] This embodiment provides a composition, comprising the following components in parts by weight: 70 parts of ursolic acid and 14 parts of quercetin.

[0088] Comparative Example 4

[0089] This embodiment provides a composition, comprising the following components in parts by weight: 15 parts of ursolic acid and 60 parts of quercetin.

[0090] Comparative Example 5

[0091] This embodiment provides a composition, comprising the following components in parts by weight: 13 parts of ursolic acid and 2 parts of quercetin.

[0092] Comparative Example 6

[0093] This embodiment provides a composition, comprising the following components in parts by weight: 8 parts of ursolic acid and 35 parts of quercetin.

[0094] Comparative Example 7

[0095] This embodiment provides a composition, comprising the following components in parts by weight: 10 parts of ursolic acid and 70 parts of quercetin.

[0096] Comparative Example 8

[0097] This embodiment provides a composition, comprising the following components in parts by weight: 75 parts of ursolic acid and 5 parts of quercetin.

[0098] Comparative Example 9

[0099] This embodiment provides a composition, comprising the following components in parts by weight: 80 parts of ursolic acid and 15 parts of quercetin.

[0100] Comparative Example 10

[0101] This embodiment provides a composition, comprising the following components in parts by weight: 85 parts of ursolic acid and 15 parts of quercetin.

[0102] The preparation methods of the embodiments and comparative examples are as follows:

[0103] (1) adding ursolic acid and quercetin in a proportion into a double-shaft high-efficiency mixer, and mixing them uniformly at a rotation speed of 500-1000 r / min;

[0104] (2) The mixture is added to a wet granulator, and granulation is performed for 10-15 minutes at a horizontal stirring blade speed of 50-100 r / min and a vertical blade speed of 500-1000 r / min to obtain a granular composition product.

[0105] Test Example 1:

[0106] 1. Test materials and test methods

[0107] 300 healthy and weight-similar Hy-Line grey laying hens aged 30 weeks were selected and randomly divided into 8 groups, with 3 replicates in each group and 20 hens in each replicate. The blank control group was fed with a corn-soybean meal type basal diet, which was a powdered compound feed prepared with reference to NRC (1994), and its composition and nutritional level are shown in Table 1. Among them, the feed of each embodiment and comparative example was added with the above-mentioned composition on the basis of the corn-soybean meal type basal diet, and the addition amount was 500g / t basal diet. The test period was 28 days.

[0108] Table 1 Feed Formula and Nutrition Level

[0109] Items Content Corn 60.75 Soybean meal 25.25 Stone Powder Flours 8.5 Soybean oil 0.5 Premix 5 Total 100 Nutrient levels Metabolizable energy ME / (kcal / kg) 3008 Crude protein CP (%) 16.42 Calcium (%) 4.24 Total phosphorus P (%) 0.56 Available phosphorus AP (%) 0.41 Lysine (%) 1.15 MethionineMet(%) 0.47 Methionine Met+cys (%) 0.75

[0110] Premix: Provides VA1200IU, VD440IU, VE7.5mg, VK0.4mg, VB per kg feed 1 0.3mg, VB 2 1.2mg, niacin 5.5mg, pantothenic acid 1.8mg, VB 6 0.55mg, VH0.03mg, folic acid 0.25mg, VB 12 0.003mg, Fe(FeSO 4 )50mg, Cu(CuSO 4)7mg, Mn(MnSO 4 )35mg, Zn(ZnSO 4 )35mg, I(KI)0.94mg, Se(NaSeO 3 )0.13mg. This nutritional value is a calculated value.

[0111] 2. Feeding methods

[0112] The experimental laying hens were raised in three-layer cages. The chicken houses and equipment were fully rinsed and strictly disinfected before the experiment. The chicken houses were temperature-controlled with electric intelligent heaters, continuous lighting, natural ventilation combined with longitudinal negative pressure ventilation. During the experimental period, the lighting, relative humidity and temperature in the house were controlled according to the requirements of laying hen feeding and management. The chicken houses were cleaned regularly and immunized according to normal immunization procedures. The chickens had free access to food and water throughout the experimental period. The temperature and humidity in the chicken house were recorded at 08:00, 12:00 and 18:00 every day.

[0113] 3. Measurement indicators and methods

[0114] 3.1 Dark-marked egg rate

[0115] Count the number of dark-striped eggs and divide by the total number of eggs.

[0116] The identification of dark-marked eggs is mainly based on visual inspection, which is to place the eggs in a light box or on a lamp holder, use strong light to penetrate the eggs, and observe some defects in the eggshell and inside the egg with the naked eye. Because the dark pattern changes the structure of the eggshell around it, this change may cause the light transmittance of the eggshell to be different under light, thus showing the dark pattern.

[0117] Figure 1 Normal egg shape. Figure 2 It is in the form of a dark-patterned egg.

[0118] 4. Test results

[0119] The results are shown in Table 2. As shown in Table 2, compared with the control group and each comparative example, adding the feed additive composition of each embodiment to the diet during the test period can reduce dark-striped eggs.

[0120] Table 2 Statistical results of dark-striped eggs

[0121]

[0122]

[0123] As shown in Table 2, the blank control group was fed with a corn-soybean meal-based diet. In conventional feeding, there was a certain dark-striped egg rate (41%). The composition of Comparative Example 1 contained only ursolic acid, which had a certain effect on reducing the dark-striped rate, and the final dark-striped egg rate was 32%. The composition of Comparative Example 2 contained only quercetin, which had a certain effect on reducing the dark-striped rate, and the final dark-striped egg rate was 34%.

[0124] In Comparative Examples 3-10, the composition contains ursolic acid and quercetin, which shows a certain effect on reducing the dark-line rate, but the composition ratio range has not been optimized, and its effect of reducing dark-line eggs is equivalent to that of the composition containing only ursolic acid or quercetin.

[0125] Table 2 shows that the compositions of Examples 1-8 have a better effect on reducing the dark-striped egg rate than Comparative Examples 1-10, and the composition of Example 3 has the best effect on reducing the dark-striped egg rate. The dark-striped egg rate in the comparative examples is about 9%-10% higher than that in the examples on average.

[0126] It is explained that the ursolic acid and quercetin compositions of Examples 1-8 after optimization show unexpected effects and can significantly reduce the dark-lined egg rate of eggs. It is explained that ursolic acid and quercetin are compounded in a certain proportion to produce a synergistic effect. The inventors also tried to use a composition of ursolic acid and isochlorogenic acid salt, as well as a composition of quercetin and isochlorogenic acid salt during the research and development process, but the results of reducing the dark-lined egg rate showed that they were not as good as the composition of ursolic acid and quercetin in the present application.

[0127] Since the rate of dark-marked eggs is closely related to egg quality, solving the problem of dark-marked eggs can improve the commodity value of eggs, enhance the overall appearance of eggs, and increase the market competitiveness of eggs. In addition, dark-marked eggs are usually accompanied by a decrease in eggshell strength, making eggs easier to break. Improving the rate of dark-marked eggs can reduce egg losses during transportation and storage, and reduce production costs.

[0128] In summary, the ursolic acid and quercetin composition of the present application can improve the quality of eggs on the basis of reducing the rate of dark-striped eggs.

[0129] Test Example 2

[0130] In this feeding test, the test was divided into a blank control group and an experimental group. The test subjects were Fengda black chickens, the mass ratio of ursolic acid to quercetin was 30:30 (Example 5), the additive dosage was 500 g / ton, and the test period was 45 days. The experimental results are shown in Table 3.

[0131] Table 3 Statistical results

[0132]

[0133]

[0134] After feeding the compound product, the rates of unqualified eggs in the two groups were 41% and 55% respectively. The unqualified rate of the experimental group supplemented with quercetin (QG) and ursolic acid was lower than that of the blank control group, with a difference of 14%. The rate of dark-marked eggs in the experimental group was 8% lower than that in the control group.

[0135] Test Example 3

[0136] This feeding test is a long-term feeding test, and the test subjects are Hailan gray laying hens. The test is divided into a blank control group, test group 1 and test group 2. Test group 1 is a group with added quercetin, the addition amount is 500g / ton, and the test period is 60 days; test group 2 is a combination product of ursolic acid and quercetin (mass ratio is 65:25, Example 6), the addition amount is 500g / ton, and the experimental period is from the beginning of laying to 490 days of laying in the late stage of laying.

[0137] During the study, it was found that long-term addition of quercetin (experimental group 1) had no positive effect on egg production. However, the combination product of ursolic acid and quercetin (experimental group 2) showed a better effect on egg production compared with the blank control group and experimental group 1.

[0138] The egg production rate of each group is shown in Figure 3 . Figure 3 It is shown that adding the composition of the present application can improve the egg production rate.

[0139] Table 4 Statistical results of dark-striped eggs

[0140]

[0141] The results in Table 4 show that the rate of dark-striped eggs in the blank control group was 58.26%. The rate of dark-striped eggs in the group fed with the ursolic acid + quercetin combination was 27.78%, significantly lower than that in the control group. The rate of dark-striped eggs in the group fed with quercetin was 46.25%, which was also lower than that in the control group, but higher than that in the group mixed with the ursolic acid and quercetin combination. The results in Table 4 show that the compound product of the ursolic acid and quercetin combination can significantly reduce the proportion of dark-striped eggs produced by laying hens.

[0142] Test Example 4

[0143] In this feeding test, the test subjects selected were Hy-Line gray laying hens in the late laying period. The test was divided into test group 3 and test group 4. Test group 3 was a combination group of ursolic acid and quercetin (13:2, comparative example 5), and test group 2 was a combination group of ursolic acid and quercetin (75:5, comparative example 8), and the additive dosage was 500g / ton.

[0144] The experimental results are shown in Table 5:

[0145] Table 5 Statistical results of dark-striped eggs

[0146]

[0147] The results in Table 5 show that the compositions of Comparative Examples 5 and 8 have a certain effect on reducing the dark-marked egg rate, but because the ratio of ursolic acid and quercetin is not the preferred ratio of the present application, the effect on reducing the dark-marked egg rate is not as good as that of the examples of the present application.

[0148] The above is a further detailed description of the present invention, which should not be regarded as a limitation on the specific implementation of the present invention. For ordinary technicians in the technical field to which the present invention belongs, simple deduction or replacement without departing from the concept of the present invention is within the protection scope of the present invention.

Claims

1. A composition, characterized in that The composition comprises the following raw materials in parts by weight: 15-70 parts of the first component and 14-60 parts of the second component; The first component is at least one of ursolic acid and ursolic acid derivatives; The second component is at least one of quercetin and a quercetin derivative.

2. The composition according to claim 1, characterized in that The composition comprises the following raw materials in parts by weight: 20-65 parts of the first component and 15-40 parts of the second component.

3. The composition according to claim 1, characterized in that The composition comprises the following raw materials in parts by weight: 20-60 parts of the first component and 20-40 parts of the second component.

4. The composition according to any one of claims 1 to 3, characterized in that The ursolic acid derivatives include ursolic acid acetate, and / or the quercetin derivatives include quercetin-7-O-glucoside and quercetin fat-soluble derivatives.

5. Use of the composition according to any one of claims 1 to 4 in any of the following: (i) Use in the preparation of a product for improving eggshell quality; (ii) Application in the preparation of feed, medicine, additives, food, health products and cosmetics.

6. A product, characterized in that The product comprises the composition according to any one of claims 1 to 4.

7. The product according to claim 6, characterized in that The products include feed, medicine, additives, food, health products and cosmetics.

8. The product according to claim 7, characterized in that When the product is feed, the content of the composition in the feed is 0.1-0.5 kilograms per ton (kg / t).

9. The product according to claim 7, characterized in that When the product is an additive, the additive includes feed additives and food additives.

10. The product according to claim 7, characterized in that The dosage form of the product is one of granules, tablets, capsules, powders, pills, suspensions, liquid preparations, and ointments.

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