An anti-aging composition for deeply repairing sensitive skin, application and anti-aging cosmetics

Abstract

The invention belongs to the technical field of cosmetics, and specifically relates to an anti-aging composition for deeply repairing sensitive skin, an application and an anti-aging cosmetic. The anti-aging composition comprises the following components in parts by weight: 1-2 parts of a spatholobiaceae extract, 3-7 parts of a Centella asiatica extract, 2-4 parts of an ergothioneine agaricus extract, and 9-12 parts of a fermented product of alpine edelweiss callus. The composition can deeply repair sensitive skin, effectively prevent and reduce wrinkles, enhance skin barrier function and hydration and moisturizing effects, and the cosmetics prepared by the composition have the effects of deep repair, anti-aging and wrinkle removal, and the like.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to an anti-aging composition for deeply repairing sensitive skin, an application thereof, and anti-aging cosmetics. Background Art

[0002] With the improvement of people's living standards and the popularization and development of the cosmetics industry, people's requirements for beauty and skin care are getting higher and higher. Compared with healthy skin, sensitive skin is more prone to aging. This is because sensitive skin is often accompanied by damaged skin barriers, which makes the skin more sensitive to external stimuli and more susceptible to various environmental insults, such as ultraviolet rays, air pollution, etc. Moreover, the level of free radicals in sensitive skin is often higher than that in normal skin. Free radicals will attack cell membranes and DNA, causing skin aging. In addition, sensitive skin has weaker skin repair ability, so when the skin is damaged, it is difficult to restore to its best condition, and it is more prone to fine lines, dry lines, spots and other problems. Therefore, sensitive skin needs more anti-aging.

[0003] When choosing anti-aging products, sensitive skin must focus on the two points of "anti-aging + repair". The ingredients must be mild, not only can improve skin roughness and light lines, but also have the effect of strong repair. Modern research has found that plant callus cells are widely used in food, medicine and cosmetics industries because of their rich secondary metabolites, naturalness, environmental friendliness and controllable conditions. Among them, alpine edelweiss callus extract can effectively fight aging, inhibit collagenase activity, prevent skin collagen degradation and loss, delay skin relaxation, reduce wrinkles, prevent skin aging and photoaging, and are widely used in cosmetics. For example, CN115364006A discloses that by adding alpine edelweiss callus extract, in combination with safflower seed oil, golden button extract, membrane pod astragalus root extract and rosemary leaf extract, the anti-aging barrier repair composition provided has outstanding loose skin lifting effect, dermal matrix repair effect and blue light damage repair effect, effectively lifts the loose skin of the neck and the tear groove area on the upper cheek, and fades crow's feet and expression lines.

[0004] At present, the products on the market that repair sensitive skin are generally a combination of two or more plant extracts. However, the combination of different plant extracts does not necessarily bring about a superposition of effects. Instead, they often antagonize each other or even aggravate skin sensitivity. Therefore, it is not easy to obtain an anti-aging combination that can deeply repair sensitive skin. Summary of the invention

[0005] In view of the deficiencies in the prior art, the purpose of the present invention is to provide an anti-aging composition for deeply repairing sensitive skin. The composition uses the fermentation product of alpine edelweiss callus as the main raw material, and is compounded with other active ingredients. It can achieve deep repair of sensitive skin, and can effectively prevent and reduce wrinkles, enhance skin barrier function and hydration and moisturizing effects.

[0006] In order to achieve the above object, the present invention discloses the following technical solutions:

[0007] In a first aspect, the present invention provides an anti-aging composition for deeply repairing sensitive skin, comprising the following components by weight: 1-2 parts of a beautiful physalis extract, 3-7 parts of a Centella asiatica extract, 2-4 parts of a thionine agaric extract, and 9-12 parts of a callus fermentation product of alpine edelweiss;

[0008] The preparation method of the alpine edelweiss callus fermentation product comprises the following steps:

[0009] S1. Wash, drain, cut and sterilize leaves of alpine edelweiss, inoculate them into an induction medium, and culture them in the dark at room temperature for 7-10 days to obtain alpine edelweiss callus; wherein the induction medium is an MS medium supplemented with 0.5 mg / L NAA;

[0010] S2, crushing the alpine edelweiss callus, adding 10-15 times the amount of 75% v / v ethanol solution, extracting at 50-60° C. for 3-5 hours, filtering, concentrating the filtrate and removing ethanol, sterilizing at 110° C. for 5-10 minutes, and obtaining a fermentation substrate;

[0011] S3, mixing the fermentation substrate with sterile water containing 5wt% sucrose in a mass ratio of 1: (20-50) to obtain a mixed solution, inoculating fermentation bacteria in the mixed solution, and fermenting at 30-35°C for 3-5 days to obtain a fermentation product; wherein the fermentation bacteria are composed of Lactococcus lactis CGMCC NO.1.12794 and Bacillus subtilis CGMCC NO.1.821, and the inoculation concentration of Lactococcus lactis CGMCC NO.1.12794 in the mixed solution is 1×10 7 -2.5×10 7 CFU / g, the inoculation concentration of Bacillus subtilis CGMCCNO.1.821 in the mixed solution is 3×10 7 -5×10 7 CFU / g, the ratio of the number of inoculated bacteria of Lactococcus lactis CGMCC NO.1.12794 and Bacillus subtilis CGMCC NO.1.821 is 1:(2-4);

[0012] S4, filtering the fermented product, collecting the filtrate and inactivating it, so as to obtain the alpine edelweiss callus fermentation product.

[0013] Preferably, in step S1, the cleaning is performed using sterile water, and the disinfection is performed using ultraviolet rays.

[0014] Preferably, in step S2, the crushing is mechanical crushing, the filtration is filtering using a filter membrane with a pore size of 100 μm, and the concentration and removal of ethanol is concentration and removal of ethanol using a rotary evaporator.

[0015] Preferably, the filtration in step S4 is performed using a 2 μm filter membrane.

[0016] Preferably, the anti-aging composition comprises the following components: 1.5 parts of Eupatorium truncatum extract, 4 parts of Centella asiatica extract, 3 parts of Agaricus ergothioneine extract, and 10 parts of alpine edelweiss callus fermentation product.

[0017] In a second aspect, the present invention provides use of the anti-aging composition described in the first aspect in the preparation of anti-aging cosmetics for sensitive skin.

[0018] Preferably, the cosmetic is any one of a cream, a lotion, a serum, an essence oil or a facial mask.

[0019] In a third aspect, the present invention provides an anti-aging cosmetic for sensitive skin, the cosmetic comprising the following components in percentage by mass: 1-5% of the anti-aging composition described in the first aspect and 95-99% of a cosmetic base.

[0020] Preferably, the cosmetic matrix contains moisturizers, emollients, emulsifiers, thickeners, preservatives, pH adjusters, fragrances, and solvents.

[0021] Preferably, the moisturizing agent is at least one of glycerin, sodium hyaluronate, allantoin, panthenol, butylene glycol, and 1,2-hexanediol.

[0022] Preferably, the emollient is at least one of squalane, isononyl isononanoate, isohexadecane, and caprylic / capric / myristic / stearic triglyceride.

[0023] Preferably, the emulsifier is at least one of polysorbate-20, polysorbate-60, cetearyl alcohol, cetearyl glucoside, polyglyceryl-10 stearate, and potassium lauryl phosphate.

[0024] Preferably, the thickener is at least one of xanthan gum, carbomer and hydroxyethyl cellulose.

[0025] Preferably, the preservative is at least one of p-hydroxyacetophenone, phenoxyethanol and ethylhexylglycerin.

[0026] Preferably, the pH regulator is at least one of arginine and triethanolamine.

[0027] Preferably, the fragrance is a daily fragrance.

[0028] Preferably, the solvent is deionized water.

[0029] In a fourth aspect, the present invention provides a method for preparing the anti-aging cosmetic for sensitive skin according to the third aspect, the preparation method comprising the following steps:

[0030] (1) Accurately weigh each raw material component according to the formula;

[0031] (2) Stir the humectant, thickener and solvent at 60°C and 300 rpm to obtain phase A;

[0032] (3) Stir the emollient and emulsifier at 60°C and 300 rpm to obtain phase B;

[0033] (4) Add phase B to phase A, emulsify and homogenize, cool to room temperature, add the anti-aging composition, preservatives, and flavor, stir evenly, add a pH adjuster, adjust the pH to 6.5-7, and obtain the anti-aging cosmetic for sensitive skin.

[0034] In the present invention:

[0035] The extract of the beautiful Ichthyophthirius can promote skin metabolism, soften skin and delay cell aging.

[0036] Centella asiatica extract contains rich active ingredients, such as madecassoside, madecassoic acid, etc., which can effectively soothe the skin and promote skin repair; at the same time, Centella asiatica extract can stimulate the skin to produce collagen, enhance skin elasticity and firmness, and delay skin aging.

[0037] Ergothioneine Agaricus tumefaciens extract has the functions of protecting skin cell DNA, reducing cell apoptosis, protecting cells from UV damage, scavenging free radicals, improving skin roughness and improving skin elasticity.

[0038] The alpine edelweiss callus fermentation product is produced by co-fermenting the alpine edelweiss callus extract with Lactococcus lactic acid and Bacillus subtilis. The co-fermentation makes the types of active ingredients richer and the concentration higher, further enhancing the anti-aging and repair ability of the alpine edelweiss callus extract.

[0039] The present invention has found that the four active ingredients of the extract of Psoralea corylifolia, the extract of Centella asiatica, the extract of Agrocybe ergothioneine and the fermentation product of callus of alpine edelweiss work synergistically to enable the anti-aging composition to effectively prevent and reduce wrinkles, enhance the skin barrier function and hydrate and moisturize the skin.

[0040] Beneficial effects of the present invention:

[0041] (1) The present invention uses Lactococcus lactis and Bacillus subtilis to jointly ferment the alpine edelweiss callus extract, thereby improving the anti-aging performance and repair effect of the alpine edelweiss callus extract, and effectively improving the anti-aging and repair effects of the system; (2) The present invention combines the fermentation product of the alpine edelweiss callus with the beautiful kelp extract, the Centella asiatica extract, and the ergothioneine matsutake extract to achieve deep repair of sensitive skin, and can effectively prevent and reduce wrinkles, improve skin barrier function and hydration and moisturizing effects. DETAILED DESCRIPTION

[0042] To better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. It should be understood by those skilled in the art that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0043] Unless otherwise specified, the test methods used in the specific implementation methods are all conventional methods; the materials, reagents, etc. used, unless otherwise specified, can be obtained from commercial channels; the percentages mentioned in the specific implementation methods, unless otherwise specified, are all mass percentages.

[0044] The extract of Pseudo-Eupatorium sphaerocephalum was purchased from Guangzhou Jucheng Chemical Co., Ltd. The extraction method was water extraction;

[0045] Centella asiatica extract: purchased from Xi'an Snote Biotechnology Co., Ltd.

[0046] Ergothioneine Agaricus matsutake extract: purchased from Bloomage Biotech Co., Ltd.;

[0047] Lactococcus lactis CGMCC NO.1.12794: purchased from China General Microbiological Culture Collection Center, with the collection number of CGMCC NO.1.12794;

[0048] Bacillus subtilis CGMCC NO.1.821: purchased from China General Microbiological Culture Collection Center, with the collection number of CGMCC NO.1.821;

[0049] Bacillus pumilus CGMCC NO.1.8714: purchased from China General Microbiological Culture Collection Center, with the collection number of CGMCC NO.1.8714;

[0050] Other raw materials were purchased commercially.

[0051] Preparation of anti-aging composition Example 1

[0052] An anti-aging composition for deeply repairing sensitive skin comprises the following components by mass: 1.5 parts of Pseudo-Euphrasia officinalis extract, 4 parts of Centella asiatica extract, 3 parts of Agaricus ergothioneine extract, and 10 parts of alpine edelweiss callus fermentation product.

[0053] The preparation method of the alpine edelweiss callus fermentation product comprises the following steps:

[0054] S1. Take leaves of alpine edelweiss, wash them with sterile water, drain, cut them, sterilize them with ultraviolet light, inoculate them in an induction medium, and culture them in the dark at room temperature for 8 days to obtain alpine edelweiss callus; wherein the induction medium is an MS medium supplemented with 0.5 mg / L NAA;

[0055] S2, mechanically crushing the obtained alpine edelweiss callus under a pressure of 500 bar, adding 10 times the amount of 75% v / v ethanol solution, extracting at 55° C. for 5 h, filtering using a filter membrane with a pore size of 100 μm, concentrating the filtrate by rotary evaporation to remove ethanol, and sterilizing at 110° C. for 5 min to obtain a fermentation substrate;

[0056] S3, mixing the fermentation substrate with sterile water containing 5wt% sucrose at a mass ratio of 1:30 to obtain a mixed solution, inoculating fermentation bacteria in the mixed solution, and fermenting at 35°C for 4 days to obtain a fermentation product; wherein the fermentation bacteria are composed of Lactococcus lactis CGMCC NO.1.12794 and Bacillus subtilis CGMCC NO.1.821, and the inoculation concentration of Lactococcus lactis CGMCC NO.1.12794 in the mixed solution is 1.5×10 7 CFU / g, the inoculation concentration of Bacillus subtilis CGMCC NO.1.821 in the mixed solution is 4.5×10 7 CFU / g, the ratio of the number of inoculated bacteria of Lactococcus lactis CGMCC NO.1.12794 and Bacillus subtilis CGMCC NO.1.821 is 1:3;

[0057] S4. Filter the fermented product using a 2 μm filter membrane, collect the filtrate and inactivate it, thereby obtaining the alpine edelweiss callus fermentation product.

[0058] The preparation method of the anti-aging composition is: uniformly mixing the components according to the proportion. Example 2

[0059] An anti-aging composition for deeply repairing sensitive skin comprises the following components by mass: 2 parts of Pseudo-Euphrasia officinalis extract, 3 parts of Centella asiatica extract, 2 parts of Agaricus ergothioneine extract, and 9 parts of alpine edelweiss callus fermentation product.

[0060] The preparation method of the alpine edelweiss callus fermentation product comprises the following steps:

[0061] S1. Take leaves of alpine edelweiss, wash them with sterile water, drain, cut them, sterilize them with ultraviolet light, inoculate them in an induction medium, and culture them in the dark at room temperature for 10 days to obtain alpine edelweiss callus; wherein the induction medium is an MS medium supplemented with 0.5 mg / L NAA;

[0062] S2, mechanically crushing the obtained alpine edelweiss callus under a pressure of 500 bar, adding 15 times the amount of 75% v / v ethanol solution, extracting at 50° C. for 5 h, filtering with a filter membrane with a pore size of 100 μm, concentrating the filtrate by rotary evaporation and removing ethanol, and sterilizing at 110° C. for 8 min to obtain a fermentation substrate;

[0063] S3, mixing the fermentation substrate with sterile water containing 5wt% sucrose at a mass ratio of 1:20 to obtain a mixed solution, inoculating fermentation bacteria in the mixed solution, and fermenting at 30°C for 5 days to obtain a fermentation product; wherein the fermentation bacteria are composed of Lactococcus lactis CGMCC NO.1.12794 and Bacillus subtilis CGMCC NO.1.821, and the inoculation concentration of Lactococcus lactis CGMCC NO.1.12794 in the mixed solution is 2×10 7 CFU / g, the inoculation concentration of Bacillus subtilis CGMCC NO.1.821 in the mixed solution is 4×10 7 CFU / g, the ratio of the number of inoculated bacteria of Lactococcus lactis CGMCC NO.1.12794 and Bacillus subtilis CGMCC NO.1.821 is 1:2;

[0064] S4. Filter the fermented product using a 2 μm filter membrane, collect the filtrate and inactivate it, so as to obtain the alpine edelweiss callus fermentation product.

[0065] The preparation method of the anti-aging composition is: uniformly mixing the components according to the proportion. Example 3

[0066] An anti-aging composition for deeply repairing sensitive skin comprises the following components by mass: 1 part of Pseudo-Euphrasia officinalis extract, 7 parts of Centella asiatica extract, 4 parts of Agaricus ergothioneine extract, and 12 parts of alpine edelweiss callus fermentation product.

[0067] The preparation method of the alpine edelweiss callus fermentation product comprises the following steps:

[0068] S1. Take leaves of alpine edelweiss, wash them with sterile water, drain, cut them, sterilize them with ultraviolet light, inoculate them in an induction medium, and culture them in the dark at room temperature for 7 days to obtain alpine edelweiss callus; wherein the induction medium is an MS medium supplemented with 0.5 mg / L NAA;

[0069] S2, mechanically crushing the obtained alpine edelweiss callus under a pressure of 500 bar, adding 12 times the amount of 75% v / v ethanol solution, extracting at 60° C. for 3 h, filtering using a filter membrane with a pore size of 100 μm, concentrating the filtrate by rotary evaporation and removing ethanol, and sterilizing at 110° C. for 10 min to obtain a fermentation substrate;

[0070] S3, mixing the fermentation substrate with sterile water containing 5wt% sucrose at a mass ratio of 1:50 to obtain a mixed solution, inoculating fermentation bacteria in the mixed solution, and fermenting at 32°C for 3 days to obtain a fermentation product; wherein the fermentation bacteria are composed of Lactococcus lactis CGMCC NO.1.12794 and Bacillus subtilis CGMCC NO.1.821, and the inoculation concentration of Lactococcus lactis CGMCC NO.1.12794 in the mixed solution is 1.2×10 7 CFU / g, the inoculation concentration of Bacillus subtilis CGMCC NO.1.821 in the mixed solution is 4.8×10 7 CFU / g, the ratio of the number of inoculated bacteria of Lactococcus lactis CGMCC NO.1.12794 and Bacillus subtilis CGMCC NO.1.821 is 1:4;

[0071] S4. Filter the fermented product using a 2 μm filter membrane, collect the filtrate and inactivate it, thereby obtaining the alpine edelweiss callus fermentation product.

[0072] The method for preparing the anti-aging composition for deeply repairing sensitive skin comprises the following steps: uniformly mixing the components according to the formula amount.

[0073] Comparative Example 1

[0074] An anti-aging composition, which differs from Example 1 in that steps S3-S4 are omitted, and the fermentation substrate obtained in step S2 is directly used to replace the alpine edelweiss callus fermentation product in Example 1 in equal amounts.

[0075] Comparative Example 2

[0076] An anti-aging composition, which differs from Example 1 in that the Bacillus subtilis in step S3 is omitted, and only Lactococcus lactis CGMCC NO.1.12794 is used for fermentation, and the inoculation concentration of Lactococcus lactis CGMCC NO.1.12794 in the mixed solution is 6×10 7CFU / g.

[0077] Comparative Example 3

[0078] An anti-aging composition, which differs from Example 1 in that Lactococcus lactis in step S3 is omitted, and only Bacillus subtilis CGMCC NO.1.821 is used for fermentation, and the inoculation concentration of Bacillus subtilis CGMCC NO.1.821 in the mixed solution is 6×10 7 CFU / g.

[0079] Comparative Example 4

[0080] An anti-aging composition, which is different from Example 1 in that the fermentation bacteria are composed of Lactococcus lactis CGMCC NO.1.12794 and Bacillus subtilis CGMCC NO.1.821, and the inoculation concentration of Lactococcus lactis CGMCC NO.1.12794 in the mixed solution is 2.4×10 7 CFU / g, the inoculation concentration of Bacillus subtilis CGMCC NO.1.821 in the mixed solution is 3.6×10 7 CFU / g, the inoculated bacterial quantity ratio of Lactococcus lactis CGMCC NO.1.12794 and Bacillus subtilis CGMCC NO.1.821 is 1:1.5.

[0081] Comparative Example 5

[0082] An anti-aging composition, which is different from Example 1 in that the fermentation bacteria are composed of Lactococcus lactis CGMCC NO.1.12794 and Bacillus subtilis CGMCC NO.1.821, and the inoculation concentration of Lactococcus lactis CGMCC NO.1.12794 in the mixed solution is 1×10 7 CFU / g, the inoculation concentration of Bacillus subtilis CGMCC NO.1.821 in the mixed solution is 5×10 7 CFU / g, the inoculated bacterial quantity ratio of Lactococcus lactis CGMCC NO.1.12794 and Bacillus subtilis CGMCC NO.1.821 is 1:5.

[0083] Comparative Example 6

[0084] An anti-aging composition, which is different from Example 1 in that the fermentation bacteria are composed of Lactococcus lactis CGMCC NO.1.12794 and Bacillus pumilus CGMCC NO.1.8714, and the inoculation concentration of Lactococcus lactis CGMCC NO.1.12794 in the mixed solution is 1.5×10 7CFU / g, the inoculation concentration of Bacillus pumilus CGMCC NO.1.8714 in the mixed solution is 4.5×10 7 CFU / g, the inoculated bacterial quantity ratio of Lactococcus lactis CGMCC NO.1.12794 and Bacillus pumilus CGMCC NO.1.8714 is 1:3.

[0085] Comparative Example 7

[0086] An anti-aging composition, which differs from Example 1 in that the extract of Pseudo-Euphrasia officinalis is missing, and the missing mass fraction is made up with Centella asiatica extract, Agrocybe truncatula extract, and alpine edelweiss callus fermentation product according to the mass fraction ratio in Example 1.

[0087] Comparative Example 8

[0088] An anti-aging composition, which differs from Example 1 in that the Centella asiatica extract is missing, and the missing mass fraction is made up with a beautiful physalis extract, a thioneine matsutake extract, and a fermented product of alpine edelweiss callus according to the mass fraction ratio in Example 1.

[0089] Comparative Example 9

[0090] An anti-aging composition, which differs from Example 1 in that thioneine Agaricus tumefaciens extract is missing, and the missing mass fraction is made up with Eupatorium tumefaciens extract, Centella asiatica extract, and alpine edelweiss callus fermentation product according to the mass fraction ratio in Example 1.

[0091] Comparative Example 10

[0092] An anti-aging composition, which differs from Example 1 in that the composition lacks alpine edelweiss callus fermentation product, and the missing mass fraction is made up with beautiful edelweiss extract, Centella asiatica extract, and ergothioneine matsutake extract according to the mass fraction ratio in Example 1.

[0093] Preparation of application examples

[0094] The raw materials and mass percentages of an anti-aging cream for sensitive skin are shown in Table 1, and the preparation method thereof comprises the following steps:

[0095] (1) Accurately weigh each raw material component according to the formula in Table 1;

[0096] (2) Stir the humectant, thickener and solvent at 60°C and 300 rpm to obtain phase A;

[0097] (3) Stir the emollient and emulsifier at 60°C and 300 rpm to obtain phase B;

[0098] (4) Add phase B to phase A, emulsify and homogenize, cool to room temperature, add the anti-aging composition, preservatives, and flavors, stir evenly, add a pH adjuster, adjust the pH to 6.5, and obtain the anti-aging cosmetic.

[0099] Table 1 Raw materials and their mass percentage of anti-aging cream

[0100]

[0101] Application Example 4-5: The difference between it and Application Example 2 is that the anti-aging composition of Example 1 in Application Example 2 is replaced by the anti-aging composition of Examples 2 and 3 in equal amounts.

[0102] Comparative Application Examples 1-10: The difference between them and Application Example 2 is that the anti-aging composition in Example 1 in Application Example 2 is replaced by the anti-aging composition in Comparative Examples 1-10 in equal amounts.

[0103] Effect test

[0104] Test Example 1: Skin Patch Test

[0105] Taking the 2015 "Technical Specification for Safety of Cosmetics" as the reference standard, the anti-aging compositions of Examples 1-3, the anti-aging compositions of Comparative Examples 1-10, the anti-aging repair creams of Application Examples 1-5, and the anti-aging repair creams of Comparative Application Examples 1-10 were subjected to skin patch tests. The specific steps were as follows: volunteers with sensitive skin aged between 28 and 45 years old were selected, and the genders were randomly determined. The volunteers were randomly divided into 28 groups, with 5 people in each group; a 40mm area was selected. 2 , a patch test device with a depth of about 1mm, put the test substance into the patch tester, the amount is 0.020-0.025g, cover the patch tester with the test substance on the back of the subject with a non-irritating cloth-based tape, and gently press it with the palm to make it evenly attached to the skin surface, continue for 24 hours, remove the test patch tester, and observe the skin reaction 30 minutes after the indentation disappears. If the result is negative, observe again 24 hours and 48 hours after the patch test.

[0106] Evaluation criteria: Level 0: negative reaction; Level 1: questionable reaction, with only slight erythema; Level 2: weak positive reaction, with erythema, infiltration, edema, and possible papules; Level 3: strong positive reaction, with erythema, infiltration, edema, possible papules, and the reaction may extend beyond the test area; Level 4: extremely strong positive reaction, with obvious erythema, severe infiltration, edema, confluent herpes, and the reaction extends beyond the test area.

[0107] Test results: Skin reactions were negative in all subjects.

[0108] The above test results show that the anti-aging composition and anti-aging cream provided by the present invention are mild and non-irritating to the skin and safe to use.

[0109] Test Example 2: Anti-aging and repair performance test

[0110] 1. Test samples: facial creams of Application Examples 1-5 and Comparative Application Examples 1-10.

[0111] 2. Test subjects: Select volunteers aged 28-45 with sensitive skin and similar skin conditions. Gender is randomly determined and all volunteers are randomly divided into groups, with 10 people in each group, and each group corresponds to one test sample.

[0112] 3. Detection method:

[0113] After cleaning their faces with water every morning and evening, volunteers evenly applied 2g of the test sample on their faces. During the test period, they did not use other cosmetics, and did not engage in long-term sun exposure or outdoor sports. This lasted for 8 weeks. The transepidermal water loss (TEWL value) of the facial skin before and after use, the moisture content of the facial skin stratum corneum, and the wrinkle removal were tracked and measured every 4 weeks, and the average value was taken.

[0114] After cleaning their faces on days 0, 28, and 56, all test subjects rested for 30 minutes in the test environment and then underwent the following tests: (1) Transepidermal water loss was measured using the MoistureMeter EpiD (Delfin, Finland) skin water loss tester, unit: g / m 2 ·h. The lower the TEWL value, the more intact the stratum corneum barrier of the skin and the less damage to the stratum corneum. (2) The stratum corneum moisture content was tested using the Comeometer (Courage & Khazaka, Germany), a dimensionless instrument. The higher the stratum corneum moisture content, the higher the moisture content of the stratum corneum. (3) The anti-wrinkle effect test used the facial image analyzer VISIA-CR to capture skin images and measure the wrinkle area ratio.

[0115] Test environment requirements: temperature 21±1℃; relative humidity 50±5%RH.

[0116] The test results are shown in Tables 2-4, where 28d△TEWL (%) in Table 2 = (28d transepidermal water loss - 0d transepidermal water loss) / 0d transepidermal water loss, 56d△TEWL (%) = (56d transepidermal water loss - 0d transepidermal water loss) / 0d transepidermal water loss; 28d stratum corneum water content change value in Table 3 (%) = (28d stratum corneum water content - 0d stratum corneum water content) / 0d stratum corneum water content, The change value of stratum corneum water content at 56 days (%) = (stratum corneum water content at 56 days - stratum corneum water content at 0 days) / stratum corneum water content at 0 days; the reduction rate of crow's feet wrinkles in Table 4 (%) = (percentage of crow's feet area on day 0 - percentage of crow's feet area on day 28 or 56) / percentage of crow's feet area on day 0 × 100%, the reduction rate of nasolabial folds (%) = (percentage of nasolabial folds area on day 0 - percentage of nasolabial folds area on day 28 or 56) / percentage of nasolabial folds area on day 0 × 100%.

[0117] Table 2 Transepidermal water loss test results

[0118]

[0119] Table 3 Skin stratum corneum moisture content test results

[0120]

[0121] Table 4 Wrinkle removal test results

[0122]

[0123] Result analysis:

[0124] According to the test results of Application Examples 1-5 and Comparative Application Examples 1-10 in Table 2-4, compared with the anti-aging composition of Comparative Examples 1-10 and the face cream of Comparative Application Examples 1-10, the anti-aging composition of Example 1-3 and the face cream of Application Examples 1-5 have better anti-aging and repair effects, which can better enhance the skin barrier of sensitive volunteers, provide long-term moisture replenishment for the skin of volunteers, and effectively remove nasolabial folds and crow's feet. According to the data of Application Example 2 and Comparative Application Examples 1-6, compared with the alpine edelweiss callus extract, the alpine edelweiss callus fermentation product has better repair and anti-aging effects, and the type of fermentation bacteria and the ratio of the number of inoculated bacteria have a significant effect on the performance of the alpine edelweiss callus fermentation product. According to the data of Application Example 2 and Comparative Application Examples 7-10, there is a certain synergistic effect between the beautiful edelweiss extract, Centella asiatica extract, ergothioneine pine mushroom extract, and alpine edelweiss callus fermentation product in the present invention.

[0125] Finally, it should be noted that the above embodiments and comparative examples are only used to illustrate the technical solution of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. An anti-aging composition for deep repairing sensitive skin, characterized in that: The invention comprises the following components by weight: 1-2 parts of Pseudo-Isodon sphaerocephala extract, 3-7 parts of Centella asiatica extract, 2-4 parts of Agaricus ergothioneine extract and 9-12 parts of alpine edelweiss callus fermentation product; The preparation method of the alpine edelweiss callus fermentation product comprises the following steps: S1. Wash, drain, cut and sterilize leaves of alpine edelweiss, inoculate them into an induction medium, and culture them in the dark at room temperature for 7-10 days to obtain alpine edelweiss callus; S2, crushing the alpine edelweiss callus, adding 10-15 times the amount of ethanol solution, extracting at 50-60°C for 3-5h, filtering, concentrating the filtrate and removing ethanol, sterilizing for 5-10min, and obtaining a fermentation substrate; S3, mixing the fermentation substrate with sterile water containing sucrose at a mass ratio of 1: (20-50) to obtain a mixed solution, inoculating fermentation bacteria in the mixed solution, and fermenting at 30-35°C for 3-5 days to obtain a fermentation product; wherein the fermentation bacteria are composed of Lactococcus lactis CGMCC NO.1.12794 and Bacillus subtilis CGMCC NO.1.821, and the inoculation concentration of Lactococcus lactis CGMCC NO.1.12794 in the mixed solution is 1×10 7 -2.5×10 7 CFU / g, the inoculation concentration of Bacillus subtilis CGMCCNO.1.821 in the mixed solution is 3×10 7 -5×10 7 CFU / g, the ratio of the number of inoculated bacteria of Lactococcus lactis CGMCC NO.1.12794 and Bacillus subtilis CGMCC NO.1.821 is 1:(2-4); S4, filtering the fermented product, collecting the filtrate and inactivating it, so as to obtain the alpine edelweiss callus fermentation product.

2. The anti-aging composition according to claim 1, characterized in that The invention comprises the following components by weight: 1.5 parts of Pseudo-Euphrasia officinalis extract, 4 parts of Centella asiatica extract, 3 parts of Agaricus ergothioneine extract and 10 parts of alpine edelweiss callus fermentation product.

3. Use of the anti-aging composition according to claim 1 or 2 in the preparation of anti-aging cosmetics for sensitive skin.

4. An anti-aging cosmetic for sensitive skin, characterized in that: The cosmetic comprises the following components in percentage by mass: 1-5% of the anti-aging composition described in claim 1 or 2 and 95-99% of the cosmetic base.

5. The anti-aging cosmetic for sensitive skin according to claim 4, characterized in that: The cosmetic matrix contains moisturizers, emollients, emulsifiers, thickeners, preservatives, pH regulators, fragrances and solvents.

6. The anti-aging cosmetic for sensitive skin according to claim 5, characterized in that: The moisturizing agent is at least one of glycerin, sodium hyaluronate, allantoin, panthenol, butylene glycol, and 1,2-hexanediol; The emollient is at least one of squalane, isononyl isononanoate, isohexadecane, and caprylic / capric / myristic / stearic triglyceride; The emulsifier is at least one of polysorbate-20, polysorbate-60, cetearyl alcohol, cetearyl glucoside, polyglyceryl-10 stearate, and potassium lauryl phosphate; The thickener is at least one of xanthan gum, carbomer and hydroxyethyl cellulose; The preservative is at least one of p-hydroxyacetophenone, phenoxyethanol, and ethylhexylglycerin; The pH regulator is at least one of arginine and triethanolamine; The fragrance is a daily fragrance; The solvent is deionized water.

7. The method for preparing the anti-aging cosmetic for sensitive skin according to claim 5 or 6, characterized in that: The preparation method comprises the following steps: (1) Accurately weigh each raw material component according to the formula; (2) Mixing the humectant, thickener and solvent to obtain phase A; (3) Stir the emollient and emulsifier evenly to obtain phase B; (4) Add phase B to phase A, emulsify and homogenize, cool to room temperature, add the anti-aging composition, preservatives and fragrance, stir evenly, add a pH adjuster, adjust the pH to 6.5-7, and obtain the anti-aging cosmetic for sensitive skin.

Images