An environmentally friendly sediment repair agent containing plant-based active ingredients, and preparation method and application thereof

Abstract

The present invention relates to the technical field of sludge remediation, and in particular to an environmentally friendly sludge remediation agent containing plant-based active ingredients, and a preparation method and application thereof. The environmentally friendly sludge remediation agent comprises the following components by weight: 20-38 parts of plant extractants, 40-65 parts of microbial agents, and 100-200 parts of adsorbent materials; the plant extractant is a mixture obtained by solvent extraction of Rhus verniciflua, Humulus, Acorus calamus, and green algae; the microbial agent includes Bacillus subtilis, Bacillus licheniformis, Nitrate bacteria, Nitrite bacteria, and Lactobacillus acidophilus; the adsorbent material is a mixture of manganese-modified biochar and zeolite. The environmentally friendly sludge remediation agent obtained by the present invention can quickly repair the sludge, does not have secondary pollution, and has a long-lasting repair ability.

Description

Technical Field

[0001] The present invention belongs to the technical field of sludge remediation, and in particular relates to an environmentally friendly sludge remediation agent containing plant-based active ingredients, and a preparation method and application thereof. Background Art

[0002] Sediment remediation refers to the process of using physical, chemical or biological methods to reduce the pollutant content in the sediment (sediment) at the bottom of polluted water bodies, reduce the release of pollutants into the water body, and improve the sediment quality and water ecological environment. Sediment is an important part of the water ecosystem. It is both a "sink" of pollutants, accumulating a large amount of heavy metals, organic matter, nutrients and other pollutants; it is also a "source" of pollutants, which will release pollutants back into the water under certain conditions, causing continuous harm to water quality and aquatic life. Existing bioremediation technology uses the life activities of specific organisms (mainly microorganisms and plants) to degrade, adsorb or transform pollutants in sediments. Although the cost is low and will not cause secondary pollution, it is difficult to produce good remediation effects in a short period of time due to the slow speed of single biological remediation.

[0003] For example, CN105712599A discloses a landscape water body sediment repair agent, which is made of the following components in parts by weight: 10-50 parts of inorganic metal salt, 5-20 parts of activated carbon, 10-80 parts of shell powder, and 10-80 parts of diatomaceous earth; the preparation method of the landscape water body sediment repair agent is to grind 10-80 parts of shell powder and 10-80 parts of diatomaceous earth, pass through a 200-mesh sieve, add 10-50 parts of inorganic metal salt and 5-20 parts of activated carbon, and stir and mix evenly. The method of using the landscape water body sediment repair agent is to prepare the repair agent into a repair agent turbid liquid with a concentration of 50g / L-100g / L, and then inject it into the sediment through a sediment injector. According to the water quality and treatment requirements, the amount of the repair agent used is controlled to be 2-10L of repair agent turbid liquid per cubic meter of landscape water. The repair agent of this application has large secondary pollution, does not have continuous repair capabilities, and has poor sustainable development.

[0004] For example, CN104445847A discloses a sediment repair agent, and each square meter of sediment includes the following components: 1000-2000g of ecological adsorbent, 500-1000g of sediment oxygenator, 100-200g of microbial growth promoter, and 1-5L of composite microorganism. It also discloses that this kind of sediment repair agent is used for a sediment locking method, and the sediment 0-3m away from the water surface-sediment interface is selected, and the sediment oxygenator, microbial growth promoter, ecological adsorbent and composite microorganism mixture and 1-5cm of sediment are added in sequence. The ecological adsorbent quickly absorbs pollutants in the sediment water body; the sediment oxygenator provides growth conditions for aerobic microorganisms in the sediment. However, the repair agent of this application contains a large amount of chemical substances, which will cause secondary pollution to the sediment, and its repair cycle is long.

[0005] In view of this, the present invention is proposed. Summary of the invention

[0006] The purpose of the present invention is to provide an environmentally friendly sediment repair agent containing plant-based active ingredients, a preparation method and an application thereof. The environmentally friendly sediment repair agent obtained by the present invention can quickly repair sediment, has no secondary pollution, and has a long-lasting repair ability.

[0007] In order to achieve the above object, the present invention provides the following technical solutions:

[0008] In a first aspect, an embodiment of the present invention provides an environmentally friendly sediment repair agent containing plant-based active ingredients, comprising the following components by weight: 20-38 parts of plant extract, 40-65 parts of microbial agent, and 100-200 parts of adsorption material;

[0009] The plant extract is a mixture of Rhus verniciflua, Humulus japonicus, Acorus calamus and Chlorella obtained by solvent extraction;

[0010] The microbial agents include Bacillus subtilis, Bacillus licheniformis, nitrate bacteria, nitrite bacteria and Lactobacillus acidophilus;

[0011] The adsorption material is a mixture of manganese-modified biochar and zeolite.

[0012] The present invention mainly repairs the bottom mud by microbial agents. The compounded microbial agents of the present invention can quickly repair the bottom mud, will not cause secondary pollution, and have a lasting repair effect, and can form a sustainable development ecosystem with the bottom mud environment.

[0013] Specifically, Bacillus subtilis and Bacillus licheniformis can secrete a variety of enzymes, such as amylase, protease and lipase. In the sediment, they can work together to decompose the macromolecular organic matter in the organic debris. For example, for the proteins, polysaccharides and fats contained in the remains of plants and animals in the sediment, these two Bacillus can work synergistically to decompose them into small molecules of amino acids, monosaccharides and fatty acids. These small molecules can be used by the bacteria themselves, and also provide nutrition for other microorganisms, promoting the growth and metabolism of microbial communities in the sediment. Moreover, they can change the structure of the sediment during growth and reproduction, making the sediment more loose. This is conducive to increasing the air permeability and water permeability of the sediment, promoting the exchange of substances between the sediment and the water body, including the exchange of oxygen and nutrients, and creating better conditions for the survival and activities of other microorganisms. In addition, Bacillus licheniformis can produce antibacterial substances, which together with Bacillus subtilis can inhibit the growth of harmful bacteria and fungi in the sediment, maintain the balance of the sediment microbial community, and prevent harmful microorganisms from interfering with the sediment remediation process. It is further explained that the nitrate bacteria and nitrite bacteria added in the present invention play a key role in the nitrogen cycle of the sediment. Nitrite bacteria can oxidize ammonia (usually from the decomposition of organic nitrogen or other ammonia-containing substances in the sediment) into nitrite, and nitrate bacteria further oxidize nitrite into nitrate. This process not only reduces the content of substances toxic to aquatic organisms such as ammonia in the sediment, but also the nitrate produced is a nutrient that can be used by plants and microorganisms, which is beneficial to the growth of plants and the metabolism of microorganisms in the sediment, and promotes the recovery of the sediment ecosystem. And because the nitrification process is an oxidation process, oxygen consumption is required. The activities of nitrate bacteria and nitrite bacteria can regulate the redox potential of the sediment, so that the oxidation environment of the sediment is improved. This is very beneficial for the growth of some aerobic microorganisms and the oxidative decomposition of organic pollutants, and can also inhibit the growth of some anaerobic harmful microorganisms (such as sulfate-reducing bacteria that produce hydrogen sulfide). More importantly, the acidophilus added in the present invention further strengthens the above-mentioned effect. Lactobacillus acidophilus can grow well in an acidic environment, and its metabolic activity can produce acidic substances such as lactic acid. In the sediment, an appropriate amount of acidic environment can promote the dissolution of some metal ions (such as heavy metal ions), making them easier to be adsorbed or precipitated and removed by other microorganisms or substances in the sediment. At the same time, the acidic environment produced by Lactobacillus acidophilus can also inhibit the growth of some harmful microorganisms that are not acid-resistant. Lactobacillus acidophilus can cooperate with Bacillus subtilis and Bacillus licheniformis. Some intermediates produced by Bacillus decomposing organic matter can provide nutrition for Lactobacillus acidophilus, and the acidic environment produced by Lactobacillus acidophilus helps the enzymes secreted by Bacillus acidophilus to work better, because the activity of some enzymes may be enhanced under acidic conditions, thereby more effectively decomposing organic matter in the sediment. The acidic environment regulated by Lactobacillus acidophilus may also have a certain effect on the growth of nitrate bacteria and nitrite bacteria.Under moderately acidic conditions, the activity of certain enzymes of nitrifying bacteria increases, thereby accelerating the rate of the nitrification process. At the same time, some nitrogen-containing compounds produced by Lactobacillus acidophilus decomposing organic matter can provide nitrogen sources for nitrifying bacteria and promote nitrification.

[0014] In a preferred embodiment, based on the mass of the plant extractant being 100 g, the viable count of the Bacillus subtilis is 1×10 10 -2×10 11 CFU / g.

[0015] In a preferred embodiment, based on the mass of the plant extractant being 100 g, the viable count of the Bacillus licheniformis is 1×10 10 -2×10 11 CFU / g.

[0016] In a preferred embodiment, based on the mass of the plant extractant being 100 g, the viable count of the nitrate bacteria is 1×10 10 -2×10 11 CFU / g.

[0017] In a preferred embodiment, based on the mass of the plant extractant being 100 g, the viable count of the nitrite bacteria is 1×10 10 -2×10 11 CFU / g.

[0018] In a preferred embodiment, based on the mass of the plant extract of 100 g, the viable count of the Lactobacillus acidophilus is 1×10 10 -2×10 11 CFU / g.

[0019] In a preferred embodiment, the preparation method of the plant extract is as follows:

[0020] The Rhus verniciflua, Humulus japonicus, Acorus calamus and Chlorella are mixed evenly, ground and sieved, and the obtained solid phase is mixed evenly with phosphate buffer solution, ultrasonicated, and centrifuged to obtain a plant extract.

[0021] In a preferred embodiment, the mass ratio of the Rhus verniciflua, Humulus japonicus, Acorus calamus and Chlorella is 1:(1-2):(2-5):(6-10).

[0022] In a preferred embodiment, the sieving uses a 100-400 mesh screen.

[0023] In a preferred embodiment, the mass ratio of the solid phase to the phosphate buffer is 1:(100-300).

[0024] In a preferred embodiment, the power of the ultrasound is 200-400 W, the time of the ultrasound is 30-60 min, and the temperature of the ultrasound is 30-40°C.

[0025] The present invention adds plant extracts on the basis of microbial agents. The plant extracts not only provide necessary nutrients for microbial metabolism, but also synergistically increase the time of sediment restoration, especially the time of initial restoration, and the continuous restoration of microorganisms prevents the sediment from causing secondary pollution.

[0026] It should be noted that the sumac contains some ingredients with antibacterial activity, such as urushiol. After extraction, these antibacterial ingredients can inhibit the growth of harmful microorganisms and reduce the number of pathogens and spoilage microorganisms in the sediment during sediment remediation. It can inhibit the growth of sulfate-reducing bacteria, which produce hydrogen sulfide in an anaerobic environment, causing the sediment to turn black and smelly. The sumac extract helps to improve the odor and color of the sediment by inhibiting their activity. More importantly, the laccase extracted from the sumac is a copper-containing oxidase. Laccase can catalyze the oxidation reaction of various organic pollutants such as phenols and aromatic amines in the sediment. It can oxidize phenolic substances in the sediment into quinones. The chemical properties of quinones are more active than phenols and are more easily further decomposed by microorganisms or other chemical processes, thereby reducing the toxicity and content of organic pollutants in the sediment. The extract of Humulus japonicus contains plant growth promoting factors. Plants generally grow around the sediment. If plants are involved (such as wetland plant remediation), these extracts can stimulate the growth and development of plant roots. A well-developed root system can better fix the bottom mud and prevent the bottom mud from resuspension. At the same time, the plant root system can absorb nutrients and some pollutants in the bottom mud, accelerating the restoration of the bottom mud. Humulus can also extract laccase, which has a degradation effect on organic pollutants. The cytochrome P450 enzyme system extracted from calamus also oxidizes and metabolizes organic pollutants in the bottom mud, changes its structure, and is more easily decomposed by microorganisms or other enzymes. In addition, the enzyme extract of calamus can optimize the structure of the microbial community, so that microorganisms that are conducive to the restoration of the bottom mud occupy a dominant position in the community. The nitrate reductase extracted from green algae can reduce the nitrate in the bottom mud to nitrite, and further convert it into ammonia, providing a nitrogen source, while reducing the nitrogen content in the bottom mud, which plays an important role in alleviating the eutrophication of the bottom mud. The alkaline phosphatase in the green algae can decompose the organic phosphorus in the bottom mud and convert it into inorganic phosphorus, which also helps to reduce the phosphorus content in the bottom mud. The interaction of the various components in the above-mentioned plant extracts can repair the bottom mud in the early stage, which speeds up the bottom mud repair time. In addition, the plant extract ingredients are safer and more environmentally friendly when used. The plant-extracted enzymes, as a kind of biological macromolecule, can be decomposed by microorganisms in the natural environment. Unlike some chemical substances, they will not remain in the environment for a long time and cause cumulative pollution. In addition, the enzymes in plants have undergone long-term evolution and adaptation, and have relatively good stability under certain conditions such as temperature and pH value. During the plant extraction process, the environment in which the enzyme is located is relatively mild, which is conducive to maintaining its activity and stability, so that it can play a better role in subsequent applications.

[0027] In a preferred embodiment, the mass ratio of the manganese-modified biochar to the zeolite is 1:2.

[0028] In a preferred embodiment, the zeolite comprises:

[0029] 20%-30% zeolite with an average pore size of 0.4-5nm;

[0030] 30%-50% zeolite with an average pore size of 10-20 nm; and

[0031] 40%-60% zeolite with an average pore size of 50-100nm.

[0032] In a preferred embodiment, the preparation method of the manganese-modified biochar is as follows:

[0033] The biochar is mixed with a potassium permanganate solution, impregnated, washed and dried, and then calcined and sieved to obtain manganese-modified biochar.

[0034] In a preferred embodiment, the mass ratio of the biochar to the potassium permanganate solution is 1:(50-100), and the content of potassium permanganate in the potassium permanganate solution is 2%-5%.

[0035] In a preferred embodiment, the immersion time is 6-12 hours.

[0036] In a preferred embodiment, the drying temperature is 50-80° C., and the drying time is 10-15 hours.

[0037] In a preferred embodiment, the calcination time is 2-5 hours, and the calcination temperature is 400-600°C.

[0038] In a preferred embodiment, the sieving is performed using a 100-200 mesh sieve.

[0039] The present invention eliminates the threat of heavy metal pollution in the bottom mud through the synergistic effect of manganese-modified biochar and zeolite with multi-level pore sizes, and the zeolite provides a better living place for microorganisms.

[0040] Specifically, the present invention modifies biochar with potassium permanganate, so that the biochar has more active sites and oxygen-containing functional groups, which is mainly caused by manganese trioxide produced after calcination. In particular, for heavy metal ions in the sediment, these active sites can be combined with them through chemical adsorption methods such as ion exchange and surface complexation. The hydroxyl groups (-OH) on the surface of manganese trioxide can react with heavy metal ions to form stable chemical bonds, thereby fixing the heavy metal ions on the surface of biochar. And biochar itself has a high specific surface area and pore structure, and this structural advantage still exists after manganese trioxide is formed on its surface. Its abundant pores can capture heavy metal ions through physical adsorption. At the same time, the presence of manganese trioxide may change the charge distribution on the surface of biochar, further enhancing the electrostatic adsorption of heavy metal ions. Therefore, the manganese-modified biochar of the present invention has a stronger heavy metal adsorption capacity than the zeolite with multi-level pore size. It should be noted that since the zeolite provided by the present invention has a multi-level pore size, the zeolite with a larger pore size is conducive to the habitat of organisms. Microorganisms can grow and reproduce inside and on the surface of the zeolite pores, avoiding excessive interference from external adverse factors (such as heavy metal toxicity and water flow impact, etc.). In addition, zeolite can adsorb and exchange nutrients, which can provide a richer nutrient source for microorganisms. The ammonium ions (NH4 + ), phosphate (PO4³ - ) and other nutrients can be used by microorganisms to promote their growth and metabolism. And because of Lactobacillus acidophilus, the acidic environment may have adverse effects, and zeolite has a certain buffering capacity and can adjust the pH of the sediment. In an acidic environment, the alkaline components in zeolite can neutralize acidic substances and prevent the pH value of the sediment from being too low, thereby providing a relatively stable living environment for microorganisms and other organisms in the sediment. In addition, zeolite with a smaller pore size competes with manganese-modified biochar for the adsorption of heavy metals, and will not cause the diversity of microorganisms to be destroyed by heavy metals.

[0041] In a second aspect, an embodiment of the present invention provides a method for preparing the above-mentioned environmentally friendly sediment repair agent containing plant-based active ingredients, comprising the following steps:

[0042] The product is obtained by mixing plant extract, microbial agent and adsorption material.

[0043] In a third aspect, an embodiment of the present invention provides the use of the above-mentioned environmentally friendly sediment repair agent containing plant-based active ingredients in repairing sediment.

[0044] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0045] 1. The present invention mainly repairs the bottom mud by microbial agents. The compounded microbial agents of the present invention can quickly repair the bottom mud, will not cause secondary pollution, and have a lasting repair effect, and can form a sustainable development ecosystem with the bottom mud environment.

[0046] 2. The present invention adds plant extracts on the basis of microbial agents. The plant extracts not only provide necessary nutrients for microbial metabolism, but also synergize with the microbial agents to increase the time of sediment repair, especially the time of initial repair. In addition, the continuous repair of microorganisms prevents the sediment from causing secondary pollution.

[0047] 3. The present invention eliminates the threat of heavy metal pollution in the sediment through the synergistic effect of manganese-modified biochar and multi-level pore size zeolite, and the zeolite provides a better living environment for microorganisms. DETAILED DESCRIPTION

[0048] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0049] Unless otherwise specified, the reagents mentioned in this example are commercially available.

[0050] Nitrate bacteria were purchased from Cangzhou Xindadi Biotechnology Co., Ltd.

[0051] Nitrite bacteria were purchased from Cangzhou Xindadi Biotechnology Co., Ltd.

[0052] Bacillus subtilis was purchased from Jiangmen Aobao Biotechnology Co., Ltd.

[0053] Bacillus licheniformis was purchased from Shenyang Hongjiuzhou Chemical Co., Ltd.

[0054] Lactobacillus acidophilus was purchased from Qingdao Genyuan Biotechnology Group Co., Ltd.

[0055] Example 1

[0056] This embodiment provides an environmentally friendly sediment repair agent containing plant-based active ingredients, including the following components by weight: 28 parts of plant extract, 53 parts of microbial agent, and 150 parts of adsorption material;

[0057] The microbial agents include Bacillus subtilis, Bacillus licheniformis, nitrate bacteria, nitrite bacteria and Lactobacillus acidophilus. Based on the mass of the plant extractant as 100g, the number of live bacteria of Bacillus subtilis is 1.6×10 11CFU / g, the number of viable bacteria of Bacillus licheniformis is 1.5×10 11 CFU / g, the number of viable bacteria of nitrate bacteria is 1.8×10 11 CFU / g, the number of viable nitrite bacteria is 2×10 11 CFU / g, the number of viable Lactobacillus acidophilus is 1×10 10 CFU / g.

[0058] The preparation method of the plant extract is as follows:

[0059] By mass, 1 part of Rhus verniciflua, 1.5 parts of Humulus japonicus, 3 parts of Acorus calamus and 8 parts of Chlorella were mixed evenly, ground and sieved through a 200-mesh sieve, and the obtained solid phase was mixed evenly with phosphate buffer in a mass ratio of 1:200. The mixture was ultrasonically irradiated at 300 W and 35 ° C for 30 min, and centrifuged to obtain a plant extract.

[0060] The adsorption material includes manganese-modified biochar and zeolite in a mass ratio of 1:2.

[0061] The zeolites include 25% of zeolites having an average pore diameter of 4 nm; 35% of zeolites having an average pore diameter of 15 nm; and 40% of zeolites having an average pore diameter of 80 nm.

[0062] The preparation method of manganese-modified biochar is as follows:

[0063] By mass, 1 part of biochar was mixed with 80 parts of potassium permanganate solution, the content of potassium permanganate in the potassium permanganate solution was 3%, and after immersion for 10 hours, it was washed and dried at 60°C for 12 hours, and then calcined at 500°C for 3 hours and passed through a 100-mesh sieve to obtain manganese-modified biochar.

[0064] The environmentally friendly sediment repair agent is obtained by mixing plant extracts, microbial agents and adsorption materials.

[0065] Example 2

[0066] This embodiment provides an environmentally friendly sediment repair agent containing plant-based active ingredients, including the following components by weight: 32 parts of plant extract, 60 parts of microbial agent, and 180 parts of adsorption material;

[0067] Among them, the microbial agents include Bacillus subtilis, Bacillus licheniformis, nitrate bacteria, nitrite bacteria and Lactobacillus acidophilus. Based on the mass of the plant extractant as 100g, the number of viable bacteria of Bacillus subtilis is 1.7×10 11 CFU / g, the number of viable bacteria of Bacillus licheniformis is 1.6×10 11 CFU / g, the number of viable bacteria of nitrate bacteria is 1.9×10 11 CFU / g, the number of viable nitrite bacteria is 2×10 11CFU / g, the number of viable Lactobacillus acidophilus is 1.3×10 10 CFU / g.

[0068] The preparation method of the plant extract is as follows:

[0069] By mass, 1 part of Rhus verniciflua, 1.5 parts of Humulus japonicus, 3 parts of Acorus calamus and 8 parts of Chlorella were mixed evenly, ground and sieved through a 200-mesh sieve, and the obtained solid phase was mixed evenly with phosphate buffer in a mass ratio of 1:200. The mixture was ultrasonically irradiated at 300 W and 35 ° C for 30 min, and centrifuged to obtain a plant extract.

[0070] The adsorption material includes manganese-modified biochar and zeolite in a mass ratio of 1:2.

[0071] The zeolites include 25% of zeolites having an average pore diameter of 4 nm; 35% of zeolites having an average pore diameter of 15 nm; and 40% of zeolites having an average pore diameter of 80 nm.

[0072] The preparation method of manganese-modified biochar is as follows:

[0073] By mass, 1 part of biochar was mixed with 80 parts of potassium permanganate solution, the content of potassium permanganate in the potassium permanganate solution was 3%, and after immersion for 10 hours, it was washed and dried at 60°C for 12 hours, and then calcined at 500°C for 3 hours and passed through a 100-mesh sieve to obtain manganese-modified biochar.

[0074] The environmentally friendly sediment repair agent is obtained by mixing plant extracts, microbial agents and adsorption materials.

[0075] Comparative Example 1

[0076] Compared with Example 1, the only difference is that no plant extractant is contained and the mass fraction of the microbial agent is changed to 81 parts.

[0077] Comparative Example 2

[0078] Compared with Example 1, the only difference is that no microbial agent is contained and the mass fraction of the plant extract is changed to 81 parts.

[0079] Comparative Example 3

[0080] Compared with Example 1, the only difference is that no adsorption material is contained.

[0081] Comparative Example 4

[0082] Compared with Example 1, the only difference is that the adsorption material is only zeolite.

[0083] Comparative Example 5

[0084] Compared with Example 1, the only difference is that the adsorption material is only manganese-modified biochar.

[0085] Comparative Example 6

[0086] Compared with Example 1, the only difference is that: the plant extract is not added with Toxicodendron vernicifluum, and the mass portion of Humulus japonicus is changed to 2.5 parts.

[0087] Comparative Example 7

[0088] Compared with Example 1, the only difference is that: Humulus japonicus is not added to the plant extract, and the mass portion of Toxicodendron suffruticosa is changed to 2.5 parts.

[0089] Comparative Example 8

[0090] Compared with Example 1, the only difference is that the plant extractant is replaced by laccase of equal weight.

[0091] Comparative Example 9

[0092] Compared with Example 1, the only difference is that Bacillus subtilis is not added to the microbial agent.

[0093] Comparative Example 10

[0094] Compared with Example 1, the only difference is that Bacillus licheniformis is not added to the microbial agent.

[0095] Comparative Example 11

[0096] Compared with Example 1, the only difference is that Lactobacillus acidophilus is not added to the microbial agent.

[0097] Performance Testing

[0098] 52 kg of bottom mud from the bottom of a fish pond was taken, and all gravel, branches and other garbage in the bottom mud were removed and mixed for use. Then, it was evenly placed in 52 glass cylinders, and water was added to test the water quality. The COD was 64 and the TOC was 16.4. The bottom mud repair agents of the embodiments and comparative examples were added respectively. Each embodiment or comparative example corresponds to 4 glass cylinder bottom muds, and the test average value was taken. The ratio of the amount of bottom mud to the amount of bottom mud repair agent added was 40:1. The COD and TOD changes after 12h, 3d, 7d, and 30d of repair were tested respectively. The results are shown in Table 1.

[0099] Table 1 Performance test results

[0100]

[0101] From the above performance test results, it can be seen that the environmentally friendly sediment repair agent obtained in Examples 1-2 can quickly repair sediment, has no secondary pollution, and has a long-lasting repair ability. In particular, the comprehensive performance of Example 2 is the most outstanding, which is mainly because the composite microbial agent and plant extract of the present invention synergistically improve the repair effect, and the homemade adsorption material of the present invention is guaranteed for the reproduction of microorganisms. The comparative example is significantly worse than the example in the corresponding performance test because it does not adopt the necessary technical solution, which better proves the irreplaceable nature of the specific technical solution of the present application in achieving technical effects and solving technical problems.

[0102] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. An environmentally friendly sediment repair agent containing plant-based active ingredients, characterized in that: The invention comprises the following components by weight: 20-38 parts of plant extract, 40-65 parts of microbial agent, and 100-200 parts of adsorption material; The plant extract is a mixture of Rhus verniciflua, Humulus japonicus, Acorus calamus and Chlorella obtained by solvent extraction; The microbial agents include Bacillus subtilis, Bacillus licheniformis, nitrate bacteria, nitrite bacteria and Lactobacillus acidophilus; The adsorption material is a mixture of manganese-modified biochar and zeolite; The preparation method of the manganese-modified biochar is as follows: The biochar is mixed with a potassium permanganate solution, impregnated, washed and dried, and then calcined and sieved to obtain manganese-modified biochar; The preparation method of the plant extract is as follows: The Rhus verniciflua, Humulus japonicus, Acorus calamus and Chlorella are mixed evenly, ground and sieved, and the obtained solid phase is mixed evenly with phosphate buffer solution, ultrasonicated and centrifuged to obtain the plant extract.

2. The environmentally friendly sediment repair agent containing plant-based active ingredients according to claim 1, characterized in that: Based on the mass of the plant extract being 100 g, the number of viable bacteria of the Bacillus subtilis is 1×10 10 -2×10 11 CFU / g; and / or, based on 100 g of the plant extract, the number of viable bacteria of the Bacillus licheniformis is 1×10 10 -2×10 11 CFU / g; and / or, based on 100 g of the plant extract, the number of viable nitrate bacteria is 1×10 10 -2×10 11 CFU / g; and / or, based on 100 g of the plant extract, the number of viable nitrite bacteria is 1×10 10 -2×10 11 CFU / g; and / or, based on 100 g of the plant extract, the number of viable Lactobacillus acidophilus is 1×10 10 -2×10 11 CFU / g.

3. The environmentally friendly sediment repair agent containing plant-based active ingredients according to claim 1, characterized in that: The mass ratio of the Rhus verniciflua, Humulus japonicus, Acorus calamus and Chlorella is 1:(1-2):(2-5):(6-10); And / or, the sieving uses a 100-400 mesh screen; And / or, the mass ratio of the solid phase to the phosphate buffer is 1:(100-300); And / or, the power of the ultrasound is 200-400 W, the time of the ultrasound is 30-60 min, and the temperature of the ultrasound is 30-40° C.

4. The environmentally friendly sediment repair agent containing plant-based active ingredients according to claim 1, characterized in that: The mass ratio of the manganese-modified biochar to the zeolite is 1:

2.

5. The environmentally friendly sediment repair agent containing plant-based active ingredients according to claim 1, characterized in that: The zeolite comprises: 20%-30% zeolite with an average pore size of 0.4-5nm; 30%-50% zeolite with an average pore size of 10-20 nm; and 40%-60% zeolite with an average pore size of 50-100nm.

6. The environmentally friendly sediment repair agent containing plant-based active ingredients according to claim 1, characterized in that: The mass ratio of the biochar to the potassium permanganate solution is 1:(50-100), and the content of potassium permanganate in the potassium permanganate solution is 2%-5%; And / or, the immersion time is 6-12h; And / or, the drying temperature is 50-80°C, and the drying time is 10-15h; And / or, the calcination time is 2-5h, and the calcination temperature is 400-600°C; And / or, the sieving is performed using a 100-200 mesh sieve.

7. A method for preparing an environmentally friendly sediment repair agent containing plant-based active ingredients as claimed in any one of claims 1 to 6, characterized in that: The following steps are involved: The product is obtained by mixing plant extract, microbial agent and adsorption material.

8. Use of the environmentally friendly sediment repair agent containing plant-based active ingredients as described in any one of claims 1 to 6 in repairing sediment.

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