Big tree nutrient solution for improving root growth and immunity

Abstract

The invention relates to a big tree nutrient solution for enhancing root system growth and immunity. The big tree nutrient solution for enhancing root system growth and immunity comprises the following components in parts by mass: 0.1-0.5 part of modified chitosan, 0.5-10 parts of salt, 0.1-2 parts of auxin, 0.1-1 part of an auxiliary agent and 50-100 parts of water, wherein the modified chitosan is sodium alginate and humic acid modified chitosan. The salt is selected from one or more of ammonium salt, phosphate, calcium salt, potassium salt, magnesium salt and ferric salt. The nutrient solution is obtained by compounding the modified chitosan, the inorganic salt, the auxin and other components, the vitality of tree cells can be stimulated, key substances needed by growth are supplied, and therefore plant growth is efficiently adjusted, meanwhile, the nutrient solution has better stability, the problems existing in the prior art are solved, and the nutrient solution has good application prospects.

Description

Technical Field

[0001] The invention relates to the technical field of agriculture, in particular to a nutrient solution for large trees capable of enhancing root growth and immunity. Background Art

[0002] Big Tree Nutrient Solution is a nutrient supplement specially designed for trees, also known as Big Tree Needle Solution or Big Tree Bag Solution. Big Tree Nutrient Solution is rich in multiple nutrients necessary for tree growth, including nitrogen, phosphorus, potassium, boron, zinc, magnesium, iron, molybdenum, etc., and incorporates beneficial biological bacteria, organic peptides or vitamins and other ingredients, which have a good promoting effect on the growth of trees.

[0003] The nutrient solution is directly infiltrated into the core of the tree trunk using the hanging needle infusion technology, which is convenient for the tree to absorb efficiently. Therefore, the nutrient solution for large trees can not only accelerate the growth of the tree's roots, branches and leaves, improve photosynthetic efficiency, but also enhance the tree's resistance and effectively resist the invasion of pests and diseases. For newly planted trees, slow-growing trees and old and weak trees, the nutrient solution for large trees also shows a rejuvenation effect, which is a key auxiliary means in garden maintenance.

[0004] At present, there are many tree nutrient solution products that are compounded with multiple inorganic salts and growth hormones, which have a certain effect in promoting tree growth. However, most of the existing technical solutions directly mix multiple components, and the results achieved are not satisfactory. Compared with other types of products, they do not show obvious advantages, and the effects of promoting growth and improving stress resistance are still difficult to reach the ideal level.

[0005] In summary, it is urgent to develop a new technical solution to solve the defects in the existing technology. Summary of the invention

[0006] Based on this, the present invention provides a nutrient solution for large trees that enhances root growth and immunity. The present invention uses modified chitosan, inorganic salts, auxin and other ingredients to compound to obtain a nutrient solution, which can stimulate the vitality of tree cells and supply key substances required for growth, thereby efficiently regulating plant growth. At the same time, the nutrient solution has better stability, solves the problems existing in the prior art, and has good application prospects.

[0007] The object of the present invention is to provide a nutrient solution for large trees that can enhance root growth and immunity, wherein the nutrient solution for large trees that can enhance root growth and immunity comprises the following components in parts by mass:

[0008]

[0009] in,

[0010] The modified chitosan is sodium alginate and humic acid modified chitosan.

[0011] Furthermore, the chitosan is vinyl chitosan.

[0012] Furthermore, the preparation method of the modified chitosan comprises the following steps:

[0013] S1, mixing sodium alginate, 3-aminoethanol vinyl ether and a catalyst, and reacting to obtain modified sodium alginate;

[0014] S2, mixing humic acid, acryloyl chloride and a catalyst, and reacting to obtain modified humic acid;

[0015] S3, mixing the modified sodium alginate, modified humic acid, chitosan and an initiator, and heating them for reaction to obtain modified chitosan.

[0016] Furthermore, in step S1, the reaction temperature is 15-30°C and the reaction time is 2-8h.

[0017] Furthermore, in step S2, the reaction temperature is 0-30°C and the reaction time is 12-36h.

[0018] Furthermore, in step S1, the catalyst is EDC and NHS; in step S2, the catalyst is organic ammonia.

[0019] Furthermore, in step S3, the heating reaction temperature is 60-80°C and the time is 6-36h.

[0020] Furthermore, in step S3, the mass ratio of the chitosan, modified humic acid and modified sodium alginate is 6:(0.5-2):(1-3).

[0021] Furthermore, the salt is selected from one or more of ammonium salts, phosphates, calcium salts, potassium salts, magnesium salts, and iron salts.

[0022] Furthermore, the auxin is selected from one or more of zeatin, zeatin riboside, and brassinolide.

[0023] Auxin is an important hormone that regulates plant growth. It plays an important role in cell division and differentiation, rhizome formation, fruit development and leaf fall, and has the effects of promoting seed germination, growth, early flowering and fruiting, etc. This type of substance has been widely used in a variety of food crops, and is more widely used in vegetables. It has a significant promoting effect on the yield and quality of crops and vegetables. However, traditional plant auxins such as indoleacetic acid and gibberellins are pesticides, so their uses are limited. The present invention uses new plant endogenous auxins such as zeatin, zeatin riboside, and brassinolide to replace traditional pesticide ingredients, expanding the application space of such products.

[0024] Furthermore, the auxiliary agent is selected from one or more of a dispersant, a stabilizer, an emulsifier, an antioxidant, and a preservative.

[0025] The present invention has the following beneficial effects:

[0026] The present invention mixes a variety of inorganic salts with auxins, modified chitosan and other ingredients to obtain a nutrient solution for large trees that enhances root growth and immunity. While meeting the plant's needs for macro- and trace elements such as nitrogen, phosphorus, potassium, iron, and magnesium, it is compounded with exogenous nutrients to achieve the effect of promoting the growth and development process of trees. Among them, the modified chitosan is obtained by the reaction of humic acid and sodium alginate with double bonds and water-soluble vinyl chitosan, so that sodium alginate and humic acid molecules are grafted into chitosan, and seaweed polysaccharides, chitosan polysaccharides and growth regulator humic acid are integrated. It is not only beneficial to enhance its own stability and play a multi-effect growth-promoting role, but also can adsorb and combine with active ingredients such as salt, zeatin, and brassinolide through intermolecular forces through a large number of hydroxyl, amino, glycoside and other groups in its molecules, further improving the stability of the nutrient solution, and is also beneficial to promote the absorption effect of nutrients, thereby quickly filling nutritional gaps, ensuring the smooth metabolism of trees, stimulating the growth of tree roots, and enhancing the tree's grip and ability to absorb water and nutrients in the soil. At the same time, it also helps to enhance the natural immunity of trees and improve the plant's ability to resist diseases and pests and environmental pressure. In summary, the tree nutrient solution of the present invention overcomes the defects existing in the prior art, has multiple uses such as promoting new roots, supplementing nutrition, rejuvenating weak trees and improving the survival rate of transplanting, and is of great significance to the development of agricultural cultivation technology. DETAILED DESCRIPTION

[0027] In order to more clearly illustrate the technical solution of the present invention, the following examples are listed. Unless otherwise stated, the raw materials, reactions and post-treatment methods shown in the examples are common raw materials on the market and technical methods well known to those skilled in the art.

[0028] The words "preferred", "preferably", "more preferably", etc. in the present invention refer to embodiments of the present invention that can provide certain beneficial effects in certain circumstances. However, other embodiments may also be preferred under the same circumstances or other circumstances. In addition, the description of one or more preferred embodiments does not imply that other embodiments are not applicable, nor is it intended to exclude other embodiments from the scope of the present invention.

[0029] It should be understood that, except in any operating examples, or where otherwise indicated, all numbers indicating, for example, the amounts of ingredients used in the specification and claims should be understood to be modified in all cases by the term "about". Therefore, unless indicated to the contrary, the numerical parameters set forth in the following specification and the appended claims are approximate values ​​that vary depending on the desired properties to be obtained by the present invention.

[0030] The method for preparing vinyl chitosan in the embodiment of the present invention comprises the following steps:

[0031] Using toluene as solvent, 68.75 g of acryloyl chloride, 76.88 g of triethylamine and 89.88 g of hydroxyethyl methacrylate were mixed, reacted at 0-5° C. for 4 h, and then washed with water, hydrochloric acid (1 M) and NaOH solution (1 M) to obtain acryloyloxyethyl methacrylate;

[0032] 6.0 g of chitosan (viscosity average molecular weight of 150,000, degree of deacetylation of 85%) was dissolved in 200 ml of an aqueous solution of 2.4 ml of glacial acetic acid, and then 200 ml of ethanol was added and stirred evenly. Then 5.64 g of the acryloyloxyethyl methacrylate was added and reacted at 50° C. for 48 h. Unreacted substances and by-products were removed by dialysis, and the product was lyophilized to obtain the product.

[0033] The preparation method of modified chitosan in the embodiment of the present invention comprises the following steps:

[0034] S1, dissolving 2 g of sodium alginate in 200 mL of dilute hydrochloric acid with a pH of 4 to obtain a sodium alginate solution, dissolving 0.96 g of EDC and 0.58 g of NHS in 5 mL of deionized water to prepare a catalyst solution, then adding the catalyst solution to the sodium alginate solution, stirring at 15° C. for 30 min, then adding 0.58 mL of 3-aminoethanol vinyl ether, continuing to stir and react at 15° C. for 5 h, using 800 mL of ethanol for precipitation, filtering, washing, and drying to obtain modified sodium alginate;

[0035] S2, using 20 mL of anhydrous dichloromethane as solvent, adding 5 g of humic acid, 1 g of acryloyl chloride and 1 g of triethylamine at 0°C, heating to 30°C, reacting for 24 hours, filtering, washing and drying to obtain modified humic acid;

[0036] S3. Using water as solvent, vinyl chitosan, modified humic acid, modified sodium alginate and ammonium persulfate in a mass ratio of 6:1:1.5:0.1 are mixed, reacted at 70° C. for 6 h under a nitrogen atmosphere, filtered, washed and dried to obtain modified chitosan.

[0037] The "parts" in the embodiments of the present invention refer to parts by mass.

[0038] Example 1

[0039] A nutrient solution for large trees that enhances root growth and immunity, the nutrient solution for large trees that enhances root growth and immunity comprising the following components in parts by mass:

[0040]

[0041] The method for preparing the tree nutrient solution for enhancing root growth and immunity comprises the following steps:

[0042] The modified chitosan and other ingredients are blended according to the above mass fractions and stirred evenly to obtain the tree nutrient solution for enhancing root growth and immunity.

[0043] Example 2

[0044] A nutrient solution for large trees that enhances root growth and immunity, the nutrient solution for large trees that enhances root growth and immunity comprising the following components in parts by mass:

[0045]

[0046]

[0047] The method for preparing the tree nutrient solution for enhancing root growth and immunity comprises the following steps:

[0048] The modified chitosan and other ingredients are blended according to the above mass fractions and stirred evenly to obtain the tree nutrient solution for enhancing root growth and immunity.

[0049] Example 3

[0050] A nutrient solution for large trees that enhances root growth and immunity, the nutrient solution for large trees that enhances root growth and immunity comprising the following components in parts by mass:

[0051]

[0052] The method for preparing the tree nutrient solution for enhancing root growth and immunity comprises the following steps:

[0053] The modified chitosan and other ingredients are blended according to the above mass fractions and stirred evenly to obtain the tree nutrient solution for enhancing root growth and immunity.

[0054] Comparative Example 1

[0055] The difference between this comparative example and Example 1 is that the modified chitosan is replaced by a mixture obtained by blending unmodified chitosan with humic acid and sodium alginate in a mass ratio of 6:1:1.5, and the other ingredients and preparation method are the same as those in Example 1.

[0056] Comparative Example 2

[0057] The difference between this comparative example and Example 1 is that in the preparation method of modified chitosan, step S2 is omitted, and in step S3, modified humic acid is not added. Other components and preparation methods are the same as those in Example 1.

[0058] Test Case

[0059] The nutrient solutions prepared in the examples and comparative examples were subjected to performance tests.

[0060] Test method:

[0061] Under the same environmental conditions, 100 golden osmanthus trees of the same specifications were transplanted and divided into 5 groups, each with 20 trees. The nutrient solutions prepared in Examples 1-3 and Comparative Examples 1-2 were respectively used for trunk injection infusion, with 1.5 L of nutrient solution injected every day for 15 days, and the trunks were sprayed with water once every morning and evening. The survival of the trees in each group was counted.

[0062] The test results are shown in Table 1.

[0063] Table 1 Test results

[0064] project Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Survival rate / % 95 95 100 85 80

[0065] According to Table 1, it can be concluded that the nutrient solution for large trees that enhances root growth and immunity according to the embodiment of the present invention has a good promoting effect on the growth of trees. It is observed that the golden osmanthus treated with the infusion of the sample of the embodiment has a larger total root length, and the root volume and specific surface area are higher than those of the comparative example, which proves that the nutrient solution for large trees prepared in the embodiment can improve the immunity of trees, increase resistance to environmental pressure, and effectively promote the root growth of plants, thereby more efficiently absorbing nutrients in the soil, thereby improving the growth efficiency of trees and the survival probability of transplantation. Therefore, the nutrient solution for large trees that enhances root growth and immunity provided by the present invention overcomes the problems existing in the prior art and is of great significance to the development of agricultural planting technology.

[0066] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-restrictive in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention.

[0067] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A nutrient solution for large trees that enhances root growth and immunity, characterized in that: The tree nutrient solution for enhancing root growth and immunity comprises the following components in parts by mass: in, The modified chitosan is sodium alginate and humic acid modified chitosan.

2. The tree nutrient solution for enhancing root growth and immunity according to claim 1, characterized in that: The chitosan is vinyl chitosan.

3. The tree nutrient solution for enhancing root growth and immunity according to claim 1, characterized in that: The preparation method of the modified chitosan comprises the following steps: S1, mixing sodium alginate, 3-aminoethanol vinyl ether and a catalyst, and reacting to obtain modified sodium alginate; S2, mixing humic acid, acryloyl chloride and a catalyst, and reacting to obtain modified humic acid; S3, mixing the modified sodium alginate, modified humic acid, chitosan and an initiator, and heating them for reaction to obtain modified chitosan.

4. The tree nutrient solution for enhancing root growth and immunity according to claim 3, characterized in that: In step S1, the reaction temperature is 15-30°C and the reaction time is 2-8h.

5. The tree nutrient solution for enhancing root growth and immunity according to claim 3, characterized in that: In step S2, the reaction temperature is 0-30°C and the reaction time is 12-36h.

6. The tree nutrient solution for enhancing root growth and immunity according to claim 3, characterized in that: In step S1, the catalyst is EDC and NHS; in step S2, the catalyst is organic ammonia.

7. The tree nutrient solution for enhancing root growth and immunity according to claim 3, characterized in that: In step S3, the heating reaction temperature is 60-80°C and the time is 6-36h.

8. The tree nutrient solution for enhancing root growth and immunity according to claim 3, characterized in that: In step S3, the mass ratio of chitosan, modified humic acid and modified sodium alginate is 6:(0.5-2):(1-3).

9. The tree nutrient solution for enhancing root growth and immunity according to claim 1, characterized in that: The salt is selected from one or more of ammonium salts, phosphates, calcium salts, potassium salts, magnesium salts, and iron salts.

10. The tree nutrient solution for enhancing root growth and immunity according to claim 1, characterized in that: The auxin is selected from one or more of zeatin, zeatin riboside and brassinolide.

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