Gemmene A synthase mutant and application thereof
CN119736285AActive Publication Date: 2025-04-01HANGZHOU NORMAL UNIVERSITY
Patent Information
- Application Number
- CN202510260070.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-06
- Publication Date
- 2025-04-01
- Estimated Expiration
- 2045-03-06
AI Technical Summary
Technical Problem
In the prior art, the chicory-derived gemane A synthase has low activity relative to the enzyme, making it difficult to adapt to the industrial production of β-elene.
Method used
CiGASlo mutants are constructed through computer-aided design and point mutation PCR methods, including site-directed mutation sites such as W305A, D337H, L415W, D477H, E485Y, R488A, etc., to improve the catalytic activity of the enzyme.
Benefits of technology
It significantly improves the enzyme activity of Gemane A synthase, enhances its application potential in β-elene production, reduces production costs, and is suitable for industrial-scale applications.
✦ Generated by Eureka AI based on patent content.
Smart Images
Figure CN119736285A_ABST
Abstract
The invention belongs to the technical field of enzyme engineering, and particularly relates to a gemmarene A synthase mutant and application thereof. According to the invention, a series of Gemmene A synthase (CiGASlo) mutants derived from cichorium intybus are obtained through computer-aided design, the CiGASlo mutants are constructed by adopting a point mutation PCR (Polymerase Chain Reaction) method, recombinant protein is further expressed and purified, and the enzymatic activity is verified through an in-vitro enzymatic reaction, so that the CiGASlo mutants of which the relative enzymatic activity is remarkably improved are screened out. According to the invention, the CiGASlo mutant with obviously improved catalytic activity can be provided, and the CiGASlo mutant is beneficial to popularization and application in industrial production processes of sesquiterpenoids such as beta-elemene and the like. The method has the advantages of mild reaction conditions, high stereoselectivity, simple catalyst treatment steps and environmental friendliness, the requirement on equipment is reduced, the production cost is remarkably reduced, and the method has a wide application prospect in industrial application.
Need to check novelty before this filing date? Find Prior Art
Citation Information
Patent Citations
(+)-germacene A high-yield gene element, genetically engineered bacterium and application thereof
CN119265151A
Germacene A synthase mutant, recombinant engineering bacterium and application
CN119506258A
Sesquiterpenoid synthase genes and their use for influencing bitterness and resistance in plants
WO2000055338A1
Cited By
Gemmene A synthetase mutant, fusion protein, engineering strain and application
CN121628885A