Method, application and product for preparing diglyceride with weight loss effect by enzymatic hydrolysis

Abstract

The present invention provides a method, application and product for preparing diglyceride with weight loss effect by enzymolysis, which belongs to the field of enzyme engineering technology. The method comprises the following steps: S1, mixing lipase, ammonium heptamolybdate tetrahydrate with water, adding glycerol precipitation, filtering, and obtaining an enzyme preparation; S2, mixing plant raw material oil, glycerol, fatty acid, sodium fatty acid with the enzyme preparation obtained in step S1, reacting under inert gas conditions, and obtaining an enzymolysate; S3, molecularly distilling the enzymolysate obtained in step S2 to obtain diglyceride; wherein the lipase is a mixture of Rhizomucor miehei lipase and Candida antarctica Aspergillus niger lipase. The present invention uses ammonium heptamolybdate tetrahydrate to load a specific lipase mixture, and at the same time optimizes the component types and ratios of the raw materials in the enzymolysis process, effectively improving the mass percentage content and diglyceride yield of enzymolysis production of diglyceride.

Description

Technical Field

[0001] The present invention belongs to the technical field of enzyme engineering, and relates to a method, application and product for preparing diglyceride with weight loss effect by enzymatic hydrolysis. Background Art

[0002] Diacylglycerol is a type of ester substance in which one fatty acid group of triglyceride is replaced by a hydroxyl group. In nature, diacylglycerol is a trace component of natural plant and animal fats and is also an intermediate product of fat metabolism. Studies have found that diacylglycerol as an edible oil helps reduce visceral fat, inhibit weight gain, and lower blood lipids. For example, Xu et al. pointed out in the article "Effect of diacylglycerol on body weight: a meta-analysis" (Asia Pac. J. Clin. Nutr. 2008; 17 (3): 415-421) that compared with edible triglyceride oil, switching to edible diacylglycerol oil is more conducive to weight loss, and the weight loss effect is related to the daily intake of diacylglycerol oil.

[0003] Chinese invention patent CN115678676A provides a flavored diglyceride oil and a preparation method thereof. The method comprises the following steps: mixing crude oil, enzyme preparation, and chelating agent, adjusting the water content, high-speed shearing, placing in a shaking table for heat preservation, filtering, and obtaining a degumming liquid; adding glycerol and lipase to the degumming liquid, placing in a vacuum reactor for reaction, collecting the reaction gas with a gas adsorbent during the reaction, centrifuging to obtain the oil layer, and obtaining a reaction product; molecularly distilling the reaction product to obtain a mixture of diglycerides and triglycerides as the heavy phase, fractionating to obtain diglyceride oil, and passing the collected reaction gas into the diglyceride oil for analysis to obtain a flavored diglyceride oil. The water content of the diglyceride oil obtained by the method is less than 0.2%, and the flavor substances will not be lost, and the flavor substance retention rate of the oil reaches more than 90%.

[0004] Chinese invention patent CN110951796A provides a method for converting fatty acid ethyl ester into diglyceride oil, comprising the following steps: (1) mixing fatty acid ethyl ester with glycerol, using lipase as a catalyst, and performing a glycerol decomposition reaction by vacuuming; (2) centrifuging the reaction product of step (1), and then performing molecular distillation to obtain purified diglycerol. This technology uses lipase as a catalyst to generate diglycerol under vacuum, and the reaction conditions are mild and simple, which solves the problem of high fatty acid content and inability to completely remove the fatty acid in the previous oil purification process.

[0005] The existing technology for producing diglycerides is mainly divided into chemical method and enzymatic method. The principle of chemical synthesis of diglycerides is to use chemical catalysts to promote the esterification reaction of glycerol and fatty acids or the transesterification reaction of oils and fats. The chemical method has low cost and is easy to produce on a large scale. The chemical method for producing diglycerides is limited by the lack of specificity of the reaction, requiring a large amount of chemical reagents / organic solvents, which does not meet the requirements of clean production, green environmental protection and food safety. The enzymatic method for producing diglycerides uses biological enzymes as catalysts for the reaction, and the reaction process includes transesterification, glycerolysis, acidolysis and other reaction processes. The conditions of the enzymatic reaction are mild and highly selective, which is suitable for the production of food-grade diglycerides. However, since the price of enzyme preparations is much higher than that of chemical catalysts, the production cost of preparing diglycerides by enzymatic method is significantly higher. Therefore, there is an urgent need for a production process to improve the yield and purity of diglycerides prepared by enzymatic method. Summary of the invention

[0006] In view of this, the purpose of the present invention is to provide a method, application and product for preparing diglyceride with weight loss effect by enzymatic hydrolysis. The present invention first prepares a new type of enzyme preparation for preparing diglyceride by enzymatic hydrolysis: by loading a mixture of Rhizomucor miehei lipase and Candida antarctica Aspergillus niger lipase on an inorganic carrier ammonium tetrahydrate heptamolybdate, and then carefully selecting glycerol as a precipitant to precipitate the obtained enzyme preparation. The enzyme preparation can improve the efficiency of the enzymatic hydrolysis reaction. The inventor unexpectedly discovered that when using the above enzyme preparation to carry out enzymatic hydrolysis to produce diglyceride, the addition of a component such as sodium fatty acid can further increase the mass percentage of diglyceride and the diglyceride yield, thereby effectively reducing the production cost of diglyceride.

[0007] To achieve the above-mentioned object of the invention, on the one hand, the present invention provides a method for preparing diglyceride with weight loss effect by enzymatic hydrolysis, comprising the following steps:

[0008] S1, mixing lipase, ammonium heptamolybdate tetrahydrate and water, adding glycerol for precipitation, filtering, and obtaining an enzyme preparation;

[0009] S2, mixing the plant raw material oil, glycerol, fatty acid, sodium fatty acid and the enzyme preparation obtained in step S1, and reacting them under inert gas conditions to obtain an enzymolyzate;

[0010] S3, subjecting the enzymatic hydrolysate obtained in step S2 to molecular distillation to obtain diglyceride;

[0011] Wherein, in step S1, the lipase is a mixture of Rhizomucor miehei lipase and Candida antarctica Aspergillus niger lipase.

[0012] Preferably, the mass ratio of the lipase, the ammonium heptamolybdate tetrahydrate, the water and the glycerol is 10:2.5-3.5:100-200:200-400.

[0013] Preferably, in step S1, the mass ratio of the Rhizomucor miehei lipase to the Candida antarctica Aspergillus niger lipase is 4-5:5-6.

[0014] Preferably, in step S2, the fatty acid is selected from at least one of linoleic acid, linolenic acid, and oleic acid, the sodium fatty acid is selected from at least one of sodium oleate and sodium stearate, and the plant raw material oil is selected from at least one of rapeseed oil, corn oil, sunflower seed oil, linseed oil, olive oil, soybean oil, and peanut oil.

[0015] As an example of the present invention, the plant raw material oil is corn oil.

[0016] As an example of the present invention, the plant raw material oil is rapeseed oil.

[0017] Preferably, in step S2, the mass ratio of the vegetable oil, glycerol, fatty acid, sodium fatty acid and the enzyme preparation obtained in step S1 is 700-900:200-400:150-250:100-150:60-80, the reaction temperature is 50-75°C, more preferably 50-70°C, and the reaction time is 1-7h.

[0018] Preferably, in step S3, the heating surface temperature in the molecular distillation is 160-185° C., the condensation surface temperature is 35-55° C., and this is repeated 3-5 times.

[0019] In another aspect, the present invention provides diglyceride prepared by the above method.

[0020] In yet another aspect, the present invention provides use of the diglyceride prepared by the above method in producing a diglyceride product.

[0021] In the last aspect, the present invention provides a diglyceride composition comprising the following components by weight:

[0022] 5.5-6.7 parts of rapeseed diglyceride, 1.3-2.5 parts of corn diglyceride, 0.65-0.75 parts of peanut oil, 0.005-0.5 parts of seabuckthorn seed oil and 0.005-0.5 parts of maple seed oil;

[0023] Among them, the low-erucic acid rapeseed diglyceride is prepared by the above method using low-erucic acid rapeseed oil as the plant raw material oil; the corn diglyceride is prepared by the above method using corn oil as the plant raw material oil; the low-erucic acid rapeseed oil is rapeseed oil with an erucic acid weight content of ≤3%.

[0024] Preferably, the peanut oil is high oleic peanut oil having an oleic acid weight content ≥ 75%.

[0025] Preferably, the diglyceride composition comprises the following ingredients by weight:

[0026] 5.8-6.4 parts of rapeseed diester, 1.6-2.2 parts of corn diester, 0.68-0.72 parts of peanut oil, 0.005-0.5 parts of sea buckthorn seed oil and 0.005-0.5 parts of Acer truncatum seed oil.

[0027] More preferably, and as an example of the present invention, the diglyceride composition comprises the following ingredients by weight: 6.1 parts of rapeseed diester oil, 1.9 parts of corn diester oil, 0.7 parts of peanut oil, 0.25 parts of sea buckthorn seed oil and 0.25 parts of Acer truncatum seed oil.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] The present invention uses tetrahydrated ammonium heptamolybdate to load a specific lipase combination, effectively increasing the mass percentage and diglyceride yield of diglyceride produced by enzymatic hydrolysis, thereby effectively reducing the production cost of diglyceride. This is specifically reflected in the following aspects:

[0030] (1) Rhizomucor miehei lipase and Antarctic Candida albicans Aspergillus niger lipase were compounded, and the compounding ratio of the two lipases was optimized to 4-5:5-6, thereby achieving synergistic enhancement of the two lipases during the enzymatic hydrolysis process and effectively increasing the mass percentage of diacylglycerol and the diacylglycerol yield.

[0031] (2) Ammonium heptamolybdate tetrahydrate, one of the metal polyoxometalates (POM), was used to load lipase to form an enzyme preparation. The inorganic components in the enzyme preparation cooperated with the lipase. Experiments showed that compared with other types of POM, ammonium heptamolybdate tetrahydrate could effectively improve the enzymatic efficiency of lipase, thereby increasing the mass percentage of diglyceride and the yield of diglyceride. At the same time, for the preparation method of the enzyme preparation, glycerol was selected as the precipitant, which reduced the effect of the precipitant on the activity of lipase.

[0032] (3) The inventors unexpectedly discovered that in the process of using the above enzyme preparation for enzymatic hydrolysis to produce diglyceride, the addition of sodium fatty acid as a raw material can further promote the enzymatic hydrolysis reaction, effectively improve the enzymatic hydrolysis efficiency, and thereby increase the mass percentage of diglyceride and the diglyceride yield. DETAILED DESCRIPTION

[0033] The following non-limiting examples can enable those skilled in the art to more fully understand the present invention, but do not limit the present invention in any way. The following content is merely an exemplary description of the scope of the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, which should also fall within the scope of the present invention.

[0034] The present invention is further described below by way of specific examples. The various chemical reagents used in the examples of the present invention are obtained through conventional commercial channels unless otherwise specified. Unless otherwise specified, the contents described below are all weight contents. Unless otherwise specified, it is understood that the process is carried out at room temperature.

[0035] In the following examples and comparative examples, the sources of some reagents are shown in Table 1:

[0036] Table 1

[0037]

[0038] Example 1

[0039] A method for preparing diglyceride comprises the following steps.

[0040] S1, 4.5 parts by mass of Rhizomucor miehei lipase, 5.5 parts by mass of Candida antarctica Aspergillus niger lipase, 3 parts by mass of ammonium heptamolybdate tetrahydrate and 150 parts by mass of water were mixed, and stirred at a speed of 350-400 rpm for 1 hour. Then 300 parts by mass of glycerol were added to produce a precipitate, and the precipitate was filtered under reduced pressure to dryness to obtain an enzyme preparation.

[0041] S2. Mix the plant raw material oil, glycerol, oleic acid, sodium oleate and the enzyme preparation obtained in step S1 in a mass ratio of 800:300:200:125:36. In a nitrogen atmosphere, heat to 50-70°C and maintain this temperature range for 6 hours. After the reaction is completed, cool to room temperature to obtain an enzymolyzate.

[0042] S3, subjecting the enzymolyzate obtained in step S2 to molecular distillation. The molecular distillation heating surface temperature is 175° C., the condensation surface temperature is 50° C., and the process is repeated 5 times, and the product is low-erucic acid rapeseed diester oil.

[0043] Example 2

[0044] Compared with Example 1, the difference is that in step S1, Rhizomucor miehei lipase is changed to 5 parts by mass, and Candida antarctica Aspergillus niger lipase is changed to 5 parts by mass, and the other steps are the same.

[0045] Example 3

[0046] Compared with Example 1, the difference is that in step S1, the Rhizomucor miehei lipase is changed to 4 parts by mass, and the Antarctic Candida antarctica Aspergillus niger lipase is changed to 6 parts by mass, and the other steps are the same.

[0047] Example 4

[0048] Compared with Example 1, the difference is that in step S1, the amount of ammonium heptamolybdate tetrahydrate is changed to 2.5 parts by mass, the amount of water is changed to 200 parts by mass, and the amount of glycerol is changed to 400 parts by mass, and the other steps are the same.

[0049] Example 5

[0050] Compared with Example 1, the difference is that in step S1, the amount of ammonium heptamolybdate tetrahydrate is changed to 3.5 parts by mass, the amount of water is changed to 100 parts by mass, and the amount of glycerol is changed to 200 parts by mass, and the other steps are the same.

[0051] Example 6

[0052] Compared with Example 1, the difference is that in step S2, the mass ratio of plant raw material oil, glycerol, oleic acid, sodium oleate and the enzyme preparation obtained in step S1 is changed to 900:200:250:100:42, and the other steps are the same.

[0053] Example 7

[0054] Compared with Example 1, the difference is that in step S2, the mass ratio of plant raw material oil, glycerol, oleic acid, sodium oleate and the enzyme preparation obtained in step S1 is changed to 700:400:150:150:30, and the other steps are the same.

[0055] Comparative Example 1

[0056] Compared with Example 1, the difference is that in step S2, ammonium heptamolybdate tetrahydrate is not used, and 153 parts by mass of water is used instead, and the other steps are the same.

[0057] Comparative Example 2

[0058] Compared with Example 1, the difference is that in step S2, ammonium heptamolybdate tetrahydrate is replaced by an equal mass portion of phosphomolybdic acid hydrate, and the other steps are the same.

[0059] Comparative Example 3

[0060] Compared with Example 1, the difference is that in step S1, the addition of glycerol is replaced by adding an equal mass portion of ethanol to precipitate the enzyme preparation, and the rest is the same.

[0061] Comparative Example 4

[0062] Compared with Example 1, the difference is that in step S2, sodium oleate is not used, and the mass ratio of rapeseed oil, glycerol, oleic acid and the enzyme preparation obtained in step S1 is 800:300:325:36, and the other steps are the same.

[0063] Comparative Example 5

[0064] Compared with Example 1, the difference is that in step S1, the amount of Rhizomucor miehei lipase is changed from 4.5 parts by mass to 10 parts by mass, and no Antarctic Candida antarctica Aspergillus niger lipase is added, and the other steps are the same.

[0065] Comparative Example 6

[0066] Compared with Example 1, the difference is that in step S1, Rhizomucor miehei lipase is not added, and the amount of Antarctic Candida antarctica Aspergillus niger lipase is changed from 5.5 parts by weight to 10 parts by weight, and the other steps are the same.

[0067] Comparative Example 7

[0068] Compared with Example 1, the difference is that in step S1, the amount of Rhizomucor miehei lipase is changed from 4.5 parts by mass to 1.2 parts by mass, and the amount of Antarctic Candida Aspergillus niger lipase is changed from 5.5 parts by mass to 8.8 parts by mass, and the other steps are the same.

[0069] Effect Example 1

[0070] Calculation of diacylglycerol yield.

[0071] Using rapeseed oil and corn oil as the plant raw material oil, respectively, rapeseed diglyceride and corn diglyceride were prepared by the methods in Examples 1 to 7 and Comparative Examples 1 to 7, respectively, and the yield and purity of diglyceride were calculated, and the calculation formula is as follows:

[0072] Diacylcerol yield = Diacylcerol yield × mass percentage of Diacylcerol ÷ amount of plant raw material oil × 100%. The mass percentage of Diacylcerol is detected by the existing liquid chromatography method.

[0073] The experimental results are shown in Table 2:

[0074] Table 2:

[0075]

[0076] The difference between Comparative Example 1 and the examples is that ammonium heptamolybdate tetrahydrate is not used to load the lipase combination. The results show that loading the lipase with ammonium heptamolybdate tetrahydrate can effectively increase the mass percentage and diglyceride yield of diglyceride in rapeseed diglyceride and corn diglyceride.

[0077] The difference between Comparative Example 2 and the embodiments is that ammonium heptamolybdate tetrahydrate is replaced by phosphomolybdic acid hydrate. The results show that the diglyceride yield of lipase loaded with phosphomolybdic acid hydrate cannot reach the diglyceride yield level after lipase is loaded with ammonium heptamolybdate tetrahydrate. This shows that not any type of polyoxometalate containing molybdenum element can achieve the effect of increasing diglyceride yield achieved by ammonium heptamolybdate tetrahydrate used in the present invention.

[0078] The difference between Comparative Example 3 and the Examples is that ethanol is used to precipitate the enzyme preparation. The results show that the process of ethanol precipitation reduces the mass percentage of diglyceride and the diglyceride yield. Ethanol as a precipitant has an adverse effect on the activity of the enzyme preparation, while using glycerol as a precipitant can overcome this adverse effect.

[0079] Comparative Example 4 is different from the embodiments in that the ingredient sodium fatty acid is not used in the enzymatic hydrolysis process. The results show that when sodium fatty acid is not used as a raw material in the enzymatic hydrolysis process, the mass percentage of diglyceride and the yield of diglyceride both decrease to a certain extent, which indicates that sodium fatty acid has a certain promoting effect on the fermentation process of diglyceride oil production.

[0080] Compared with the examples, the difference between Comparative Examples 5 and 6 is that different types of lipase are used. The mass percentage content and diglyceride yield of the enzymatic hydrolysis methods of Comparative Examples 5 and 6 are not as good as those of the examples, which shows that the compounding of the two enzymes, Miehei Mucor lipase and Antarctic Candida Aspergillus niger lipase, can improve the mass percentage content and diglyceride yield of enzymatic hydrolysis to a certain extent, and the two can play a synergistic role. Compared with Comparative Examples 5 and 6, although the two enzymes, Miehei Mucor lipase and Antarctic Candida Aspergillus niger lipase, are compounded in Comparative Example 7, the ratio is 1.2:8.8. Although Comparative Example 7 has a certain improvement over Comparative Examples 5 and 6 in terms of the mass percentage content and diglyceride yield of diglyceride, the effect is still not as good as the effect obtained by the enzymatic hydrolysis method of each embodiment.

[0081] Application Examples

[0082] A diglyceride composition.

[0083] 6.1 parts by weight of rapeseed diglyceride, 1.9 parts by weight of corn diglyceride, 0.7 parts by weight of peanut oil, 0.25 parts by weight of seabuckthorn seed oil and 0.25 parts by weight of Acer truncatum seed oil were mixed to obtain diglyceride edible oil.

[0084] Among them, the preparation methods of the above-mentioned rapeseed diglycerol and corn diglycerol are both the methods described in Example 1: the plant raw material oil in step S2 is replaced with rapeseed oil and corn oil, and enzymatic hydrolysis is performed using the enzymatic hydrolysis method provided in Example 1 to obtain rapeseed diglycerol and corn diglyceride respectively.

[0085] Application comparison

[0086] A composition. Compared with the application example, the only difference is that the low-erucic acid rapeseed diglyceride is replaced by an equal weight portion of low-erucic acid rapeseed oil, and the corn diglyceride is replaced by an equal weight portion of corn oil, and the rest are the same.

[0087] Effect Example 2

[0088] Mouse weight loss experiment

[0089] In the following animal experiments, C57BL / 6J mice were purchased from Weitonglihua and were raised in independent ventilation cages (IVC) for mice, with 5 mice per cage. The environmental parameters of the animal room were recorded during the feeding period, and no other species of animals were raised in the same room during the experiment. The feeding environment is: temperature 20-26 ° C, relative humidity 40%-70%, ventilation using an independent ventilation cage system for ventilation, lighting is alternating light and dark every 12 hours, and the lights are turned off at 7 pm every day and turned on at 7 am the next day. The SPF mouse maintenance feed sterilized by cobalt-60 irradiation is provided by Shanghai South Model Organisms Technology Co., Ltd.; high-fat feed (fat energy supply ratio 60%) is provided by Beijing Keao Xieli Feed Co., Ltd.; oil replacement feed is provided by Beijing Keao Xieli Feed Co., Ltd., and the diglyceride composition provided in the application example of the present invention and the composition provided in the application comparison example are used to replace the vegetable oil added in the high-fat feed.

[0090] Five-week-old male SPF-grade C57BL / 6J mice were used as experimental animals and randomly divided into groups of 5 mice each as follows.

[0091] Blank control group: fed with mouse maintenance feed every day.

[0092] Obesity modeling group: fed with high-fat diet daily.

[0093] Application Example / Application Comparative Example Group: The mice were fed with a high-fat diet every day for four consecutive weeks, and then fed with the fat substitute feed of the Application Example or the fat substitute feed of the Application Comparative Example.

[0094] The weight of mice was measured every 2 weeks starting from the 0th week of the experiment. After the weight measurement was completed in the 12th week of the experiment, the mice in each group were killed by cervical dislocation, and the inguinal subcutaneous fat, epididymal fat, perirenal fat, and scapular brown fat were collected and their total wet weight was weighed, and the body fat percentage of the mice was calculated based on the weight of the mice.

[0095] Body fat percentage = total wet weight of collected fat ÷ mouse body weight × 100%.

[0096] The body weights of mice in each group at the 0th, 2nd, 4th and 6th weeks of the experiment are shown in Table 3 (mean ± standard deviation, the P value between the blank control group and the obesity modeling group was calculated using an unpaired t-test, and P < 0.05 indicated that the two groups of data had significant differences).

[0097] Table 3

[0098]

[0099] The treatments of the application example and the application comparison group were the same as those of the obesity modeling group, and there was no significant difference in body weight between 0 and 4 weeks and the obesity modeling group.

[0100] As can be seen from the data in Table 3, the weight of mice in the obesity modeling group was greater than that of mice in the blank control group, and the difference was significant (P < 0.01).

[0101] The body weights of mice in each group at the 6th, 8th, 10th and 12th weeks of the experiment are shown in Table 4 (mean ± standard deviation, P value was analyzed and calculated using One-way ANOVA).

[0102] Table 4

[0103]

[0104] in, Indicates that the data in the same column are significantly different from the obesity modeling group and P < 0.05. Indicates that the data in the same column are significantly different from the obesity modeling group and P < 0.01. Indicates that the data in the same column are significantly different from the obesity modeling group and P < 0.0001. # Indicates that the data in the same column are significantly different from the blank control group except for the obesity modeling group and P < 0.05. ### It means that the data in the same column are significantly different from the blank control group except for the obesity modeling group and P < 0.001.

[0105] The average body fat percentage of mice in each group is shown in Table 5.

[0106] Table 5

[0107]

[0108] It can be seen that the diglyceride composition provided in the application examples of the present invention has the effect of significantly reducing the body weight and body fat rate of mice, while the composition in the application examples does not have such an effect.

[0109] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A method for preparing diglyceride with weight loss effect by enzymatic hydrolysis, characterized in that: The following steps are involved: S1, mixing lipase, ammonium heptamolybdate tetrahydrate and water, adding glycerol for precipitation, filtering, and obtaining an enzyme preparation; S2, mixing the plant raw material oil, glycerol, fatty acid, sodium fatty acid and the enzyme preparation obtained in step S1, and reacting them under inert gas conditions to obtain an enzymolyzate; S3, subjecting the enzymatic hydrolysate obtained in step S2 to molecular distillation to obtain diglyceride; Wherein, in step S1, the lipase is a mixture of Rhizomucor miehei lipase and Candida antarctica Aspergillus niger lipase; In step S1, the mass ratio of the lipase, the ammonium heptamolybdate tetrahydrate, the water and the glycerol is 10:2.5-3.5:100-200:200-400; In step S1, the mass ratio of the Rhizomucor miehei lipase to the Antarctic Candida Aspergillus niger lipase is 4-5:5-6; In step S2, the mass ratio of the plant raw material oil, glycerol, fatty acid, sodium fatty acid and the enzyme preparation obtained in step S1 is 700-900: 200-400: 150-250: 100-150: 30-42.

2. The method according to claim 1, characterized in that In step S2, the fatty acid is selected from at least one of linoleic acid, linolenic acid, and oleic acid, the sodium fatty acid is selected from at least one of sodium oleate and sodium stearate, and the plant raw material oil is selected from at least one of rapeseed oil, corn oil, sunflower seed oil, linseed oil, olive oil, soybean oil, and peanut oil.

3. The method according to claim 1, characterized in that In step S2, the reaction temperature is 50-75° C., and the reaction time is 1-7 h.

4. The method according to claim 1, characterized in that: In step S3, the heating surface temperature in the molecular distillation is 160-185° C., the condensation surface temperature is 35-55° C., and this is repeated 3-5 times.

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