Lily cultivation method

Abstract

The invention discloses a lily cultivation method which comprises the following steps in sequence: breaking seed dormancy at low temperature, disinfecting the surfaces of the seeds, germinating the seeds and transplanting the seeds, and in the step of seed germination, the lily seeds subjected to surface disinfection are spread on the surface of a water-containing fabric for germination culture; when cotyledons germinated from the lilies grow until petioles are 1.0-3.0 cm in length, the lilies are transplanted into a seedling culture medium to be cultured. The cultivation method provided by the invention is simple to operate, and the germination rate of the lily seeds, the seedling emergence uniformity and the survival rate of transplanted seedlings can be improved.

Description

Technical Field

[0001] The present application relates to the technical field of plant cultivation, and in particular to a method for cultivating lilies. Background Art

[0002] Lily (Lilium spp.) is a monocotyledonous perennial bulbous flower of the genus Lilium in the family Liliaceae. It generally grows in cool and humid places, prefers slightly acidic soil, and has strong adaptability. Lily flowers have high ornamental value and are often used as flower bouquets. The flowers can also be used as spices because they contain aromatic oils. In addition, the underground bulbs of lilies have good nutritional and nourishing effects. The bulbs of some germplasms have both edible and medicinal functions and can be used to relieve cough and expectorant, nourish yin and moisten the lungs, etc., making them ideal Chinese medicinal materials. Lilies have extremely high economic benefits, so they have been widely cultivated in my country.

[0003] In lily cultivation and production, when the initial material is seeds, the main methods currently used are tissue culture and soil sowing. However, the former has the problems of cumbersome operation, high cultivation cost, and low survival rate after transplantation due to drastic changes in the cultivation environment. The latter has the problems of long time consumption and poor consistency of seedling emergence. Summary of the invention

[0004] In view of the existing technical problems, the present application provides a lily cultivation method, which is simple to operate and can improve the cultivation survival rate and uniformity.

[0005] The lily cultivation method of the present application comprises the following steps in sequence: breaking seed dormancy at low temperature, sterilizing the seed surface, seed germination and transplanting. The seed germination is to spread the surface-sterilized lily seeds on a water-containing fabric surface for germination culture. When the sprouted cotyledons of the lily grow to a petiole length of 1.0 to 3.0 cm, they are transplanted to a seedling medium for culture.

[0006] Several optional methods are also provided below, but they are not intended to be additional limitations on the above-mentioned overall solution, but are merely further supplements or preferences. Under the premise that there are no technical or logical contradictions, each optional method can be combined with the above-mentioned overall solution separately, and multiple optional methods can also be combined.

[0007] Optionally, the water content per unit area of ​​the fabric is 0.078 to 0.088 g / cm 2 .

[0008] Optionally, the fabric is gauze.

[0009] Optionally, the gauze is folded to form a multi-layer structure.

[0010] Optionally, the seed germination is carried out in a sealed environment.

[0011] Optionally, the lily seeds are spread at intervals on the surface of the water-containing fabric.

[0012] Optionally, water is added to the fabric during the germination culture process to keep its surface moist.

[0013] Optionally, the germination culture conditions are: in the dark and at 19-21°C.

[0014] Optionally, the low-temperature breaking of seed dormancy is: placing the lily seeds in an environment of 3 to 5° C. for 2 to 4 weeks.

[0015] Optionally, the formula of the seedling medium is peat: rice husk: wood ash: coconut brick = 5:1:1:1.

[0016] Compared with the prior art, the present invention adopts water-containing fabric to germinate and cultivate seeds, which has a fast germination speed, can obtain a seed germination rate of 91-100%, has good uniformity of emergence and a high survival rate of seedlings after transplanting, and has a good cultivation effect of lilies. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 is a flow chart of a method for cultivating lily in one embodiment;

[0018] Figure 2 This is a graph showing the effect of different culture conditions on the germination rate of Lilium formosanum 'Leishan No. 1' in Comparative Examples 1 to 3 and Application Example 1;

[0019] Figure 3A This is a graph showing the growth of the new Lilium formosanum 'Leishan No. 1' in Comparative Example 1 after being cultured on germination medium for 15 days;

[0020] Figure 3B This is a graph showing the growth of the new Lilium formosanum 'Leishan No. 1' in comparative example 2 after being cultured on germination medium for 15 days;

[0021] Figure 3C This is a graph showing the growth of the new Lilium formosanum 'Leishan No. 1' in comparative example 3 after being cultured on germination medium for 15 days;

[0022] Figure 3D This is a graph showing the growth of the new Lilium formosanum 'Leishan No. 1' in Application Example 1 after being cultured on germination medium for 15 days;

[0023] Figure 4A This is a diagram showing the emergence of the new Lilium formosanum 'Leishan No. 1' after transplanting in Comparative Example 2;

[0024] Figure 4B This is a diagram showing the emergence of the new Lilium formosanum 'Leishan No. 1' after transplanting in Comparative Example 3;

[0025] Figure 4CThis is a picture of the emergence of the new Lilium formosanum 'Leishan No. 1' after transplanting in Application Example 1;

[0026] Figure 5 This is a graph showing the effect of different culture conditions on the germination rate of Luding lily in Application Example 2 and Comparative Example 4;

[0027] Fig. 6A This is a graph showing the growth of the Luding lily in Example 4 after 30 days of culture on the germination medium;

[0028] Figure 6B This is a graph showing the growth of the Luding lily in Application Example 2 after 30 days of culture on the germination medium;

[0029] Fig. 7A This is a diagram of the emergence of the Luding lily after transplanting in Example 4;

[0030] Figure 7B This is a diagram of the germination of Luding lily after transplanting in Application Example 2. DETAILED DESCRIPTION

[0031] The following will be combined with the embodiments of the present application to clearly and completely describe the technical solution of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0032] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those generally understood by those skilled in the art to which this application belongs. The terms used herein in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0033] Seed sowing and propagation is one of the main methods for cultivating lilies. Lily seeds have dormancy characteristics under natural conditions and germinate very slowly. It takes a long time for them to germinate after sowing. During this period, the seeds are prone to deterioration and mildew, resulting in an extremely low germination rate, which affects the cultivation effect of lilies. The existing technology mainly induces seed germination through tissue culture and then transplants it, but this method has the problems of cumbersome operation, poor germination effect and low transplant survival rate.

[0034] See also Figure 1 In order to solve the existing technical problems, the present application provides a lily cultivation method, comprising the following steps in sequence:

[0035] (1) Low temperature breaks seed dormancy;

[0036] (2) Surface disinfection of seeds;

[0037] (3) Seed germination: Spread the surface-sterilized lily seeds on a water-containing fabric surface for germination and cultivation, and transplant them when the cotyledons of the lilies grow to a petiole length of 1.0 to 3.0 cm;

[0038] (4) Transplantation: Transplant to seedling medium for cultivation.

[0039] The present application adopts water-containing fabric as a seed germination medium, which can obtain a seed germination rate of 91.00-100.00%, a fast germination speed, uniform germination, and a high survival rate of seedlings after transplanting, so the cultivation effect of lily is good.

[0040] Among them, the seeds should be selected from the current year with mature and full grains, embryos and no mildew. The specific operation of breaking seed dormancy by low temperature is: place the seeds in an environment of 3 to 5 degrees Celsius for 2 to 4 weeks, such as placing them in a refrigerator at 4 degrees Celsius for 2 to 4 weeks. Before low temperature treatment, the seeds are pre-wrapped in paper envelopes to prevent the seeds from getting damp and mildewing during low temperature storage.

[0041] In one embodiment, surface disinfection comprises the following steps:

[0042] First disinfection: Soak the seeds in a mixture of Tween-20, detergent and water, and wash the seeds after soaking;

[0043] Second disinfection: Use 75% (v / v) ethanol solution and 2% (w / v) sodium hypochlorite to soak and disinfect the seeds in turn, and wash the seeds after disinfection.

[0044] Specifically, in the first disinfection, the volume ratio of the mixed solution of Tween-20, detergent and water is (0.8-1.2):(5-7):(600-800); preferably 1:6:700, and the mixture is shaken gently during the soaking process, and the soaking time is 30 minutes. Among them, the detergent is the oil-removing high-efficiency liquid detergent of Shanghai Hehuang Baimao Co., Ltd. After the soaking is completed, the seeds are rinsed with running water. Before rinsing, all the seeds are wrapped and fixed with gauze, and then placed under running water for rinsing.

[0045] The second disinfection is carried out on a clean bench. The seeds are soaked and disinfected with 75% ethanol solution for 30 seconds, and 2% sodium hypochlorite for 8 minutes. The seeds are shaken gently during the soaking process. After the disinfection is completed, each seed is rinsed 5 to 6 times with sterile water, and each rinse time is 1 to 2 minutes. The rinsed seeds are placed on filter paper to absorb and set aside.

[0046] In step (3), the fabric itself has tiny pores, similar to capillaries, which can cause capillary phenomena. The adsorption force generated by the capillaries can move liquids to solids. In this application, the water-containing fabric refers to the fabric itself absorbing a certain amount of water, so that the fabric is moist but not dripping, and the water content should be appropriate. Generally, too much water can easily cause seeds to rot, and too little water is not conducive to seed germination. In a specific embodiment, the water content per unit area of ​​the fabric is 0.078-0.088 g / cm 2 , preferably 0.085 g / cm 2 .

[0047] The fabric may be gauze. Further, the gauze is folded to form a multi-layer structure, for example, the gauze is folded to form a 4-layer structure.

[0048] In one embodiment, the seed germination is carried out in a sealed environment to prevent the moisture of the fabric from volatilizing quickly and to ensure the moisture required for the seed germination. Specifically, the fabric is spread on the bottom of the bottom dish of the culture dish, and an appropriate amount of water is added to the fabric to make the fabric wet. After the seeds are inoculated, the top cover is covered and sealed with a sealing film to seal the entire culture dish, and then the germination culture is carried out. The fabric, water, bottom dish and top cover used are sterilized at high temperature in advance.

[0049] The bottom area of ​​the bottom dish is the minimum area of ​​the gauze, and the height of the gauze edge not higher than the bottom dish is the maximum area, that is, 56.72~76.94cm 2 , preferably 70cm 2 .

[0050] To ensure the germination rate of the seeds, the lily seeds are spread on the surface of the water-containing fabric at intervals to avoid mutual influence, so that each lily seed can obtain approximately equal opportunities for germination and growth. In a specific embodiment, the density of lily seed inoculation is 5 to 6 cm 2 / grain.

[0051] The seeds are in direct contact with the surface of the fabric to absorb water. In order to keep the surface of the fabric moist, in one embodiment, the fabric is replenished with water during the germination process. For example, the fabric is replenished with water in time according to the observation of the surface condition of the fabric. The specific operation is: if signs of dryness are observed on the surface of the fabric every 3 to 5 days, 1 to 2 mL of sterile water is added to the fabric using a pipette on a clean bench.

[0052] In order to quickly promote seed germination, the conditions for germination culture are: the temperature is set at 19-21°C, and the seeds are cultured in the dark until no new seeds germinate for 3-5 consecutive days, and the cotyledons grow to a petiole length of 1.0-3.0 cm, then they can be transplanted. Too low or too high a temperature is not conducive to lily seed germination, and dark conditions are more conducive to embryo development. The length of the cotyledon petiole is an important criterion for judging whether the seeds can be transplanted. A cotyledon petiole length shorter than 1.0 cm is not conducive to the survival of the seedlings after transplantation.

[0053] In one embodiment, the matrix formula used during transplanting is peat: rice husk: plant ash: coconut brick = 5:1:1:1, which can improve the uniformity of seedling emergence and the survival rate of seedlings. Lilies like loose, fertile, and well-drained soil, and adding a certain ratio of rice husk and coconut brick is conducive to maintaining the air permeability of the soil.

[0054] The technical solution of this application is further described below in combination with application examples and specific parameters. The test materials and reagents used in the following application examples can be obtained from commercial channels unless otherwise specified. If no specific technology or conditions are specified in the application examples, they can be carried out according to the technology or conditions described in the literature in this field or according to the product instructions.

[0055] In the following application examples, the lily seed varieties used are the new iron cannon lily 'Leishan No. 1' and the Luding lily, both of which are seeds harvested in the same year.

[0056] Application Example 1

[0057] (1) Seed selection: Select seeds of Lilium longifolia 'Leishan No. 1' that are mature, plump, complete and free of mold.

[0058] (2) Breaking seed dormancy at low temperature: Place the seeds in a paper envelope and place in a 4°C refrigerator for 3 weeks.

[0059] (3) Perform two disinfection steps in sequence:

[0060] First disinfection: prepare a mixture of Tween-20, detergent and water in a volume ratio of 1:6:700, and soak the seeds after low-temperature dormancy in the mixture for 30 minutes. During the soaking period, shake gently and rinse with running water for 2 hours;

[0061] Second disinfection: On an ultra-clean workbench, soak the seeds that have completed the first disinfection in 75% (v / v) ethanol solution for 30 seconds, then soak the seeds in 2% (w / v) sodium hypochlorite solution for 8 minutes, shaking gently during the soaking process, and finally rinse the seeds with sterile water 6 times, 2 minutes each time.

[0062] (4) Seed germination:

[0063] On the clean bench, lay a folded 4-layer gauze inside a bottom dish (8.5 cm in diameter, 1.4 cm in height). When laying, the gauze evenly covers the entire bottom surface of the bottom dish, and the edge can be close to the inner wall of the bottom surface and not higher than the height of the bottom dish. The area of ​​the gauze laid is 70 cm 2 Then add a certain amount of sterile water to the gauze to make the whole gauze moist, and its water content is 0.085g / cm 2 ;

[0064] Then, 10 selected seeds are spread on the surface of each gauze at intervals, and after the spreading is completed, the top cover is put on, and a sealing film is used to wrap and seal the bottom dish and the top cover;

[0065] The temperature was set at 19-21°C, and the germination culture was carried out in dark conditions for 15 days. During the germination process, the dryness of the gauze surface was observed. If signs of dryness were observed on the gauze surface every 3-5 days, the top cover was opened on the clean bench, and 1-2 mL of sterile water was added to the gauze using a pipette. When the sprouted cotyledons grew to a petiole length of 1.0-3.0 cm, they could be transplanted.

[0066] (5) Transplanting: Fill each hole of the seedling tray with a seedling medium, the formula of the seedling medium is: peat: rice husk: plant ash: coconut brick = 5:1:1:1, transplant the seedlings that meet the transplanting requirements to the seedling tray, and then place the whole in a shaded place indoors for cultivation at a cultivation temperature of 24° C. Water the seedling medium once every 3 to 5 days, and water the seedling medium thoroughly. After 60 days of cultivation, the transplant survival rate is calculated;

[0067] In the early stage of seedling cultivation, a transparent and breathable film is used to cover the top of the seedling tray to form a greenhouse to keep warm. In the early stage of transplanting, the greenhouse is closed. Every 3 days, 1 / 4 of the film is rolled up for ventilation, and the film is completely rolled up after 2 weeks.

[0068] Comparative Example 1

[0069] (1) Seed selection: Select seeds of Lilium longifolia 'Leishan No. 1' that are mature, plump, complete and free of mold.

[0070] (2) Perform two disinfection steps in sequence:

[0071] First disinfection: prepare a mixture of Tween-20, detergent and water in a volume ratio of 1:6:700, and soak the seeds after low-temperature dormancy in the mixture for 30 minutes. During the soaking period, shake gently and rinse with running water for 2 hours;

[0072] Second disinfection: On an ultra-clean workbench, soak the seeds that have completed the first disinfection in 75% (v / v) ethanol solution for 30 seconds, then soak the seeds in 2% (w / v) sodium hypochlorite solution for 8 minutes, shaking gently during the soaking process, and finally rinse the seeds with sterile water 6 times, 2 minutes each time.

[0073] (3) Seed germination:

[0074] Prepare a medium containing 4.43g / L MS, 1.0mg / L 6-BA, 0.2mg / L NAA, 30g / L sucrose, 3g / L phytagel, pH 5.8, select 10 seeds with full embryos, place them on filter paper to dry, inoculate them on each medium, and culture them in the dark at 20±1℃. After 15 days of culture, the germination rate was counted.

[0075] (4) Transplanting: Fill each hole of the seedling tray with a seedling medium, the formula of which is: peat: rice husk: plant ash: coconut brick = 5:1:1:1, transplant the seedlings that meet the transplanting requirements into the seedling tray, and then place the whole in a shaded place indoors for 60 days at a cultivation temperature of 24° C. Water the seedling medium once every 3 to 5 days, and water the seedling medium thoroughly;

[0076] In the early stage of seedling cultivation, a transparent and breathable film is used to cover the top of the seedling tray to form a greenhouse to keep warm. In the early stage of transplanting, the greenhouse is closed. Every 3 days, 1 / 4 of the film is rolled up for ventilation, and the film is completely rolled up after 2 weeks.

[0077] Comparative Example 2

[0078] The only difference from Comparative Example 1 is that the culture medium in step (3) is replaced by a culture medium containing 4.43 g / L MS, 30 g / L sucrose, 3 g / L phytagel, pH 5.8, i.e., no hormones.

[0079] Comparative Example 3

[0080] The only difference from Comparative Example 1 is that the culture medium in step (3) is replaced by a culture medium containing 4.43 g / L MS, 3 g / L phytagel, pH 5.8, i.e., no hormones and carbon sources.

[0081] Application Example 2

[0082] (1) Seed selection: Select Luding lily seeds that are mature, plump, complete and free of mold.

[0083] (2) Breaking seed dormancy at low temperature: Place the seeds in a paper envelope and place in a 4°C refrigerator for 3 weeks.

[0084] (3) Carry out the first disinfection and the second disinfection in sequence:

[0085] First disinfection: prepare a mixture of Tween-20, detergent and water in a volume ratio of 1:6:700, and soak the seeds after low-temperature dormancy in the mixture for 30 minutes. During the soaking period, shake gently and rinse with running water for 2 hours;

[0086] Second disinfection: On an ultra-clean workbench, soak the seeds that have completed the first disinfection in 75% (v / v) ethanol solution for 30 seconds, then soak the seeds in 2% (w / v) sodium hypochlorite solution for 8 minutes, shaking gently during the soaking process, and finally rinse the seeds with sterile water 6 times, 2 minutes each time.

[0087] (4) Seed germination:

[0088] On the clean bench, lay a folded 4-layer gauze inside a bottom dish (8.5 cm in diameter, 1.4 cm in height). When laying, the gauze evenly covers the entire bottom surface of the bottom dish, and the edge can be close to the inner wall of the bottom surface and not higher than the height of the bottom dish. The area of ​​the gauze laid is 70 cm 2 Then add a certain amount of sterile water to the gauze to make the whole gauze moist, and its water content is 0.085g / cm 2 ;

[0089] Then, 10 selected seeds are spread on the surface of each gauze at intervals, and after the spreading is completed, the top cover is put on, and a sealing film is used to wrap and seal the bottom dish and the top cover;

[0090] The temperature was set at 19-21°C, and germination culture was carried out in dark conditions for 30 days. During the germination process, the dryness of the gauze surface was observed. If signs of dryness were observed on the gauze surface every 3-5 days, the top cover was opened on the clean bench, and 1-2 mL of sterile water was added to the gauze using a pipette. When the sprouted cotyledons grew to a petiole length of 1.0-3.0 cm, they could be transplanted.

[0091] (5) Transplanting: Fill each hole of the seedling tray with a seedling medium, the formula of which is: peat: rice husk: plant ash: coconut brick = 5:1:1:1, transplant the seedlings that meet the transplanting requirements into the seedling tray, and then place the whole in a shaded place indoors for 60 days at a culture temperature of 24° C. Water the seedling medium once every 3 to 5 days, and water the seedling medium thoroughly;

[0092] In the early stage of seedling cultivation, a transparent and breathable film is used to cover the top of the seedling tray to form a greenhouse to keep warm. In the early stage of transplanting, the greenhouse is closed. Every 3 days, 1 / 4 of the film is rolled up for ventilation, and the film is completely rolled up after 2 weeks.

[0093] Comparative Example 4

[0094] (1) Seed selection: Select Luding lily seeds that are mature, plump, complete and free of mold.

[0095] (2) Perform two disinfection steps in sequence:

[0096] First disinfection: Soak in a mixture of Tween-20, detergent and water for 30 minutes, shaking gently and flushing with running water for 2 hours;

[0097] Second disinfection: Treat the seeds with 75% ethanol solution for 30 seconds and 2% sodium hypochlorite solution for 8 minutes on a clean bench, then rinse with sterile water for 6 times, 2 minutes each time.

[0098] (3) Seed germination:

[0099] Prepare a medium containing 4.43 g / L MS, 3 g / L plant gel, and pH 5.8, select 10 seeds with full embryos, place them on filter paper to dry, inoculate them on each medium, and culture them in the dark at 20±1°C. After 30 days of culture, the germination rate was counted.

[0100] (4) Transplanting: Fill each hole of the seedling tray with a seedling medium, the formula of the seedling medium is: peat: rice husk: plant ash: coconut brick = 5:1:1:1, transplant the seedlings that meet the transplanting requirements into the seedling tray, and then place the whole in a shaded place indoors for cultivation at a cultivation temperature of 24° C. Water the seedling medium once every 3 to 5 days, and water the seedling medium thoroughly. After 60 days of cultivation, the transplant survival rate is calculated;

[0101] In the early stage of seedling cultivation, a transparent and breathable film is used to cover the top of the seedling tray to form a greenhouse to keep warm. In the early stage of transplanting, the greenhouse is closed. Every 3 days, 1 / 4 of the film is rolled up for ventilation, and the film is completely rolled up after 2 weeks.

[0102] Results and Analysis:

[0103] Application Example 1 was compared with Comparative Example 1, Comparative Example 2, and Comparative Example 3 on the same day, and the single-factor ANOVA test was performed using the Duncan multiple comparison method of IBM SPSS Statistics 25 software. * indicates significant difference (p<0.05); *, **, and *** are significantly different at the 0.05, 0.01, and 0.001 levels, respectively.

[0104] Figure 1 , Figure 2 , Figure 3A ~D, Figure 4A~C and Table 1 statistically analyzed the effects of different culture conditions on the seed germination rate, seedling uniformity and transplant survival rate of Lilium formosanum 'Leishan No. 1'. Finally, it was determined that the culture dish containing 4 layers of sterile gauze and sterile water was the optimal culture scheme, with a germination rate of up to 100.00%.

[0105] Figure 5 , Fig. 6A ~B, Fig. 7A ~B and Table 2 statistically show the effects of different culture conditions on the germination rate, uniformity of seedling emergence and transplant survival rate of Luding lily seeds. Finally, it was determined that the culture dish containing 4 layers of sterile gauze and sterile water was the optimal culture scheme, with a germination rate of up to 91.00%.

[0106] Table 1 Seed germination rate, uniformity of seedling emergence and survival rate after transplantation of Lilium formosanum ‘Leishan No. 1’

[0107]

[0108]

[0109] Note: " / " in the table indicates that the petiole length of the seedlings in comparative example 1 did not meet the transplanting requirements. "n" indicates the number of statistics.

[0110] Table 2 Seed germination rate, uniformity of seedling emergence and transplant survival rate of Luding lily

[0111]

[0112] Note: “n” in the table indicates the number of statistics.

[0113] The cultivation method provided by the present application does not mechanically damage lily seeds, and can quickly break dormancy, shorten seedling time, and improve seed germination rate, effectively solving the problems of low germination rate of existing lily seeds, low seedling quality and low uniformity of seedling emergence, greatly improving seed germination rate, and indirectly improving breeding efficiency. The specific advantages are:

[0114] (1) The disinfection and sowing procedures of lily seeds are simple, the toxicity to seeds is small, and the contamination rate can be reduced to 0.00%. Compared with traditional culture medium aseptic sowing and soil sowing, the present invention has lower cost, shorter time consumption, and saves a lot of manpower and material resources.

[0115] (2) Before culturing, the seeds were placed in a 4°C refrigerator for 2 to 4 weeks. Low temperature treatment effectively broke the seed dormancy and greatly increased the seed germination rate.

[0116] (3) When the lily seed culture dish contained 4 layers of sterile gauze and sterile water, the germination rate of the new formosan lily 'Leishan No. 1' seeds was as high as 100.00% at 15 days after sowing, and the germination rate of the Luding lily seeds was as high as 91.00% at 30 days after sowing. The seeds grew well and had a high uniformity of emergence.

Claims

1. A method for cultivating lily, comprising the following steps in sequence: breaking seed dormancy at low temperature, disinfecting the seed surface, seed germination and transplanting, characterized in that: The seed germination is to spread the surface-sterilized lily seeds on a water-containing fabric surface for germination culture, and when the sprouted cotyledons of the lily grow to a petiole length of 1.0 to 3.0 cm, transplant them into a seedling medium for culture.

2. The cultivation method according to claim 1, characterized in that The water content per unit area of ​​the fabric is 0.078-0.088 g / cm 2 .

3. The cultivation method according to claim 1, characterized in that The fabric is gauze.

4. The cultivation method according to claim 3, characterized in that: The gauze is folded to form a multi-layer structure.

5. The cultivation method according to claim 1, characterized in that: The seed germination is carried out in a sealed environment.

6. The cultivation method according to claim 1, characterized in that: The lily seeds are spread at intervals on the surface of the water-containing fabric.

7. The cultivation method according to claim 1, characterized in that: During the germination culture, water was added to the fabric to keep its surface moist.

8. The cultivation method according to claim 1, characterized in that: The conditions for the germination culture are: in a dark environment and at a temperature of 19-21°C.

9. The cultivation method according to claim 1, characterized in that: The low temperature method for breaking seed dormancy is as follows: placing the lily seeds in an environment of 3 to 5° C. for 2 to 4 weeks.

10. The cultivation method according to claim 1, characterized in that: The formula of the seedling medium is peat: rice husk: plant ash: coconut brick = 5:1:1:1.

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