A suspension seed coating agent containing thiophanate-methyl, thiophanate-methyl and metalaxyl-M and its application

Abstract

The invention discloses a suspension seed coating agent containing thiofuran (A), thiophanate-methyl (B) and metalaxyl-M (C) and its application, wherein the mass ratio of the active ingredients thiofuran, thiophanate-methyl and metalaxyl-M is 1-5:1-5:1-10. The invention compound thiofuran, thiophanate-methyl and metalaxyl-M to prepare a suspension seed coating agent, which has the advantages of good film-forming property, good coating uniformity, uniform drug release, safety, low toxicity, good water resistance and the like. Various diseases on peanuts can be effectively prevented and treated by seed dressing, such as peanut root rot and peanut white rot, which are the most serious pests of peanuts. The composition has good rapid effect, and the mutual cooperation under different action mechanisms can effectively delay the development of drug resistance of diseases, and greatly reduce the number of times farmers use drugs while preventing and curing diseases. In addition, the composition can effectively improve the growth environment of peanuts in the root stage and seedling stage, improve health and efficiency, thereby improving the germination rate and yield of peanut seeds, and has a good application prospect.

Description

Technical Field

[0001] The invention relates to the technical field of pesticide compounding, and in particular to a suspended seed coating agent containing thiophanate-methyl, thiophanate-methyl and metalaxyl-M and an application thereof. Background Art

[0002] Thifluzamide, chemical formula: C 13 H6Br2F6N2O2S is a thiazole amide fungicide. Its main mechanism of action is to inhibit succinate dehydrogenase in the tricarboxylic acid cycle of pathogens, leading to bacterial death. It has strong systemic conductivity and long-lasting effect. Thifluzamide is active against pathogenic fungi such as Rhizoctonia, Puccinia, Ustilago, Tinkle, Corticotropha, and Pyricularia, and is especially effective against diseases caused by Basidiomycetes such as sheath blight and damping-off.

[0003] Benziothiazolinone, a heterocyclic compound of isothiazole, is a highly effective, low-toxic, broad-spectrum fungicide, and also a protective fungicide. Its mechanism of action is mainly: it combines with anions on the surface of bacterial and fungal cell membranes or reacts with sulfhydryl groups, destroys the synthesis system of proteins and cell membranes, inhibits the reproduction of bacteria and fungi, interferes with the metabolism of pathogenic bacteria cells, causes physiological disorders, and leads to the death of pathogens. This compound has a good preventive and control effect on rot, ulcer, anthracnose, black spot, black shank, leaf spot, downy mildew, etc. of crops caused by bacteria and fungi, and is very safe for crops.

[0004] Metalaxyl-m, chemical formula: C 15 H 21NO4 is the R-type active enantiomer of methoxyl. It is a broad-spectrum fungicide that inhibits the synthesis of proteins and ribosomal RNA in fungi and is also used to study plant diseases caused by pathogens of the Oomycete family. Its mechanism of action mainly includes the following four aspects: Protection and treatment: Metalaxyl-M has protection, treatment and eradication effects on diseases. When used before crops are infected, it can protect crops from pathogens; when used after crops are infected, it can prevent the spread and development of pathogens in the plant body, and has a significant inhibitory effect on the expansion of the initial lesions of potato late blight and the production of zoospores. Internal absorption and transport effect: Metalaxyl-M has a strong two-way internal absorption and transport effect on crops, and penetrates and conducts quickly in the plant body. After the agent is absorbed by the roots and stems, it is transported to the leaves and young and tender tissues newly grown after the application of the drug along with the water circulation in the plant body, protecting the leaves and young and tender tissues from pathogens; after being absorbed by the upper leaves, it can be conducted to the base leaves and tissues, inhibiting the reproduction and spread of pathogens in the group. Long lasting effect: for seed treatment or root irrigation, the lasting effect is about 1 month; and for foliar spray, it lasts about 15 days. Strong selectivity: Metalaxyl-M is only effective against Oomycete diseases, and has special effects on downy mildew, phytophthora, and pythium.

[0005] Peanut root rot is caused by infection by Fusarium of the Ascomycota. It is commonly known as "rat tail" and root rot. The cause of the disease is a variety of Fusarium, mainly Fusarium solani, Foxysporum Schlecht, Froseum Seth, Ftricinctum Corde, Fmoniliforme Sheld, etc., all of which belong to the Ascomycota. It harms the entire growth period of peanuts and can be infected in any growth period. The disease is severe during the peak flowering and podding period, with an incidence rate of about 10%, and can reach 20% to 30% in severe cases. If the soil humidity is too high, the bacteria of blight or bacterial wilt will reproduce faster, and the roots will lack oxygen, which will invade the pathogenic bacteria from the roots, making it easy to be infected with root rot. The soil is cultivated in a rough manner, the quality of land preparation is poor, and the topsoil is uneven, resulting in waterlogging. The soil fertility is insufficient, the peanuts grow slowly, the plants are short, and the assimilation is poor. Once there is long-term rain, they are easily infected with root rot.

[0006] Peanut white rot (Sclerotium rolfsii Sacc), also known as Sclerotium rolfsii, belongs to the subphylum Ascomycota and is a disease of peanuts. Peanuts can be infected by white rot fungi at all growth stages, mainly harming the stems, fruit stalks, pods and roots of the plants. Peanut white rot is an important soil-borne fungal disease of peanuts, which is common in major peanut producing countries such as the United States and India. White rot occurs in most peanut production areas in China. Due to changes in farming systems, large-scale promotion of high-yield new varieties and changes in climatic conditions, the occurrence and damage have become more serious year by year, and it has developed into one of the important diseases of peanuts and has become an important factor restricting peanut yield and quality.

[0007] In agricultural production, the pathogens carried by the crop seeds themselves, the pathogens in the soil, the pathogens spread by the air in the seedling stage, etc. often cause the reduction of crop yield and quality, or even the loss of grains, which has become a major limiting factor in agricultural production. In the face of this problem, the best solution is undoubtedly to use a suspended seed coating agent to coat the seeds to be sown, which not only ensures the germination rate of the seeds, but also promotes the rapid growth of the seedlings, so that the crops mature early and increase the yield. At the same time, this patent has screened out a combination of thiophanate-methyl, thiophanate-methyl and metalaxyl-M by consulting a large amount of literature and completing relevant indoor and field efficacy tests. It can effectively prevent and control peanut root rot and peanut white rot, and there is currently no report on a suspended seed coating agent containing thiophanate-methyl, thiophanate-methyl and metalaxyl-M. Summary of the invention

[0008] The present invention aims to provide a suspension seed coating agent containing thiophanate-methyl, thiophanate-methyl and metalaxyl-M and its application. The seed coating agent is a water-based suspension seed coating agent prepared by using a corresponding suspension agent processing technology, and is used for coating peanut seeds. The composition has a good bactericidal effect and can improve the growth environment of peanuts in the root stage and seedling stage, thereby increasing the seed germination rate and enhancing health care. It can also effectively prevent and control the occurrence of diseases such as peanut root rot and peanut white rot, and has a synergistic effect in preventing and controlling diseases.

[0009] To achieve the purpose of the present invention, the present invention adopts the following technical solutions:

[0010] One aspect of the present invention relates to a suspension seed coating agent containing thiophanate-methyl, thiophanate-conazole and metalaxyl-M, the seed coating agent is composed of active ingredients and auxiliary ingredients, the active ingredients are thiophanate-methyl, thiophanate-conazole and metalaxyl-M, wherein the mass ratio of the three active ingredients is 1-5:1-5:1-10.

[0011] Preferably, the mass ratio of thiophanate-methyl, thiophanate-methyl and metalaxyl-M is 1-4:1-4:1-5.

[0012] In the suspended seed coating agent of the present invention, the auxiliary ingredients include one or more of a dispersant, a wetting agent, a penetrant, an antifreeze agent, a thickener, a defoaming agent, a film former, a preservative, a colorant, a pH regulator, and deionized water.

[0013] In one embodiment of the present invention, the suspended seed coating agent formula includes: 3% to 20% active ingredient, 3% to 6% dispersant, 2% to 5% wetting agent, 0% to 4% penetrant, 1% to 6% antifreeze agent, 0.1% to 3% thickener, 0.3% to 1% defoamer, 0.5% to 3% film former, 0.1% to 0.5% preservative, 3% to 17% colorant, 0% to 3% pH adjuster, and 30% to 80% deionized water.

[0014] In the suspension seed coating agent of the present invention, wherein:

[0015] The dispersant is selected from the group consisting of alkylbenzene sulfonates, alkylnaphthalene sulfonates, cresol sulfonic acid, naphthol sulfonic acid formaldehyde condensation products, N-methyl fatty acyl taurate, lignin sulfonate, alkylphenol polyoxyethylene ether formaldehyde condensation product sulfates, fatty alcohol polyoxyethylene ether sulfates, glycerol fatty acid polyoxyethylene ethers, vegetable oil (castor oil) ethylene oxide adducts and derivatives thereof, and alkylphenol polyoxyethylene ethers and formaldehyde condensation products, or a mixture of two or more thereof;

[0016] The wetting agent is selected from one or a mixture of two or more of nonylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, alkanolamide polyoxyethylene ether, phosphate or sulfate ester salt, alkyl polyoxyethylene ether succinate sulfonate, terminal olefin sulfonate, alkylbenzene sulfonate, alkylnaphthalene sulfonate, low molecular weight naphthalene sulfonate formaldehyde condensate, lignin sulfonate, polycarboxylate, etc.;

[0017] The penetrant is selected from one or a mixture of two or more of fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether, phosphate ester salt or sulfate ester, azone, fast penetrant T, sulfonated castor oil, heptamethyl trisiloxane polyoxyethylene ether, etc.;

[0018] The antifreeze agent is selected from one of ethylene glycol, propylene glycol, glycerol, urea, ammonium sulfate, sodium sulfate, sodium chloride, or a mixture of two or more thereof;

[0019] The thickener is selected from one or a mixture of two or more selected from xanthan gum, magnesium aluminum silicate, alginate, polyvinyl alcohol, polyvinyl pyrrolidone, sodium hydroxymethyl cellulose, sodium polyacrylate, etc.;

[0020] The defoamer is selected from one or a mixture of two or more of an organosilicon defoamer, a polyether defoamer, a high carbon alcohol, tributyl phosphate, and a polysiloxane defoamer;

[0021] The film-forming agent is selected from polyvinyl alcohol (PVA), polyethylene glycol (PEG), polyvinyl acetate, polyvinyl pyrrolidone (PVP), rosin, polystyrene, styrene-acrylic emulsion, acrylic resin polymer, carboxymethyl cellulose, gelatin, gum arabic, xanthan gum, sodium alginate, agar, polysaccharide polymer compounds, cellulose derivatives, etc., or a mixture of two or more thereof;

[0022] The preservative is one or more of benzisothiazolinone, sodium benzoate, sorbitol and propyl polyhydroxybenzoate;

[0023] The colorant is selected from one of alkaline rose essence, acid scarlet, magenta, water-based rose red, carmine, etc., or a mixture of two or more thereof.

[0024] The pH regulator is selected from sodium hydroxide, triethanolamine, glacial acetic acid, hydrochloric acid, citric acid or phosphoric acid.

[0025] It is known that not all compounds are compatible after compounding and can be prepared into the pesticide formulations required in agriculture. The present invention, through a large number of experimental studies, screens for suitable formulations and optimal formulation ratios, and combines corresponding indoor and field efficacy tests, and finally harvests a new type of pesticide composition suspension seed coating agent. The suspension seed coating agent of the present invention is mainly used for peanut seeds, and is coated and mixed with seeds before planting, so that the occurrence of common diseases such as root rot and white rot on peanuts can be effectively prevented and controlled, and has a synergistic effect, promotes seed germination and improves germination rate, and also promotes the rapid growth of seedlings, so that crops mature early and increase yields.

[0026] Another object of the present invention is to provide a method for preparing the above-mentioned suspended seed coating agent, which comprises first accurately weighing the active ingredients, auxiliary ingredients (except film-forming agents and colorants), and deionized water according to a ratio, and then homogenizing and dispersing them in a high-speed shear emulsifying disperser. After about 30 minutes, zirconium beads with a weight ratio of 1:1.3 are added for sand grinding for 1.5 hours, and the sample is collected and filtered. Finally, a film-forming agent and a colorant are added thereto for homogenous stirring, and the material is discharged after mixing evenly.

[0027] Another object of the present invention is to provide the use of the above-mentioned suspension seed coating agent for preventing and controlling fungal and bacterial diseases of crops. In the above-mentioned use, the crop is preferably peanut; and the disease is preferably peanut root rot and peanut white rot.

[0028] Compared with the prior art, the present invention has the following advantages:

[0029] 1. The present invention compounds thiophanate-methyl, thiophanate-methyl and metalaxyl-M in a certain ratio. Since the three have different bactericidal action mechanisms, the bactericidal spectrum is effectively expanded. At the same time, the three also have good compatibility, can effectively prevent and control the occurrence of peanut root rot and peanut white rot, have a synergistic effect, improve the root environment of peanuts, effectively promote the growth and development of the peanut root system, root and strengthen the seedlings, and improve the survival rate of the plants.

[0030] 2. The seed coating agent of the present invention can prevent and treat both fungal and bacterial diseases, which reduces the number of times the drug is used to a certain extent, reduces the drug cost and labor cost, and has little pollution to the environment. DETAILED DESCRIPTION

[0031] In order to make the purpose, technical solutions and advantages of the present invention clearer and more understandable, the technical solutions involved in the present invention will be described in detail below in combination with specific indoor biological tests and field efficacy test examples, but the present invention is not limited to the following examples. It should be pointed out that any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be based on the attached claims.

[0032] 1. Indoor bioassay activity determination

[0033] Example 1: Screening of compound ratios of thiophanate-methyl, thiophanate-methyl and metalaxyl-M against peanut root rot and determination of co-toxicity coefficient

[0034] 1. Test conditions

[0035] 1.1 Test targets

[0036] Peanut root rot (Fusarium oxysporum) was collected and isolated from Zhongmou County, Henan Province and stored at 4°C.

[0037] 1.2 Culture conditions

[0038] Before the assay, the bacteria were inoculated on PSA medium plates and pre-cultured at 25°C for 5 days. Mycelium blocks with a diameter of 5 mm were taken from the edge of the colony for assay.

[0039] 1.3 Instruments and Equipment

[0040] Biochemical incubator, SW-CJ-2F double-sided clean bench, eppendorf pipette, oven, hole puncher, culture dish, etc.

[0041] 2. Experimental Design

[0042] 2.1 Test Agents

[0043] 98% thiophanate-methyl technical (Shandong United Pesticide Industry Co., Ltd.), 95% thiophanate-methyl technical (Shaanxi Xida Huate Technology Industry Co., Ltd.), 92% metalaxyl-M technical (Antao Microbiology (Jiangsu) Co., Ltd.).

[0044] 2.2 Experimental treatment

[0045] 2.2.1 Dose setting

[0046] The test drugs thiofuranamide, thiophanate-methyl and metalaxyl-M were prepared into mother solutions with dimethyl sulfoxide and diluted with 2% Tween 80 sterile water. Then thiofuranamide, thiophanate-methyl and metalaxyl-M were compounded in 15 ratios of 4:1:5, 4:1:3, 4:1:1, 2:1:5, 2:1:3, 2:1:1, 1:1:5, 1:1:3, 1:1:1, 1:2:5, 1:2:3, 1:2:1, 1:4:5, 1:4:3, 1:4:1, etc. After the drugs were preparatory tested, the above-designed series of ratios were added to the PSA culture medium to prepare drug-containing plates, and the culture medium without drugs was set as a blank control (sterile water containing 2% Tween 80 instead of water).

[0047] 2.2.2 Experimental Repetition

[0048] Each concentration treatment was repeated 4 times.

[0049] 3. Test methods

[0050] The mycelium growth rate method was used. The pre-prepared mycelium blocks were selected and inoculated on PSA medium plates with different drug concentrations and cultured at 25°C for 5 days. The colony diameter was measured by the cross method, the average colony diameter was calculated, and the EC50 of different drugs on the strain was determined. The co-toxicity coefficient (CTC) of the mixture was calculated by the co-toxicity coefficient calculation method. The synergistic effect of the mixture was determined, and the specific calculation method was as follows:

[0051] Take a single agent in the mixture as the standard agent (usually the one with the lower EC 50) and calculate:

[0052] Single-dose toxicity index = standard drug EC 50 / a single-dose EC 50×100

[0053] Measured toxicity index (ATI) = EC50 value of standard single agent / EC50 value of mixed agent × 100

[0054] Theoretical toxicity index (TTI) = toxicity index of single agent A × the proportion of single agent A in the mixture + toxicity index of single agent B × the proportion of single agent B in the mixture + toxicity index of single agent C × the proportion of single agent C in the mixture

[0055] Co-toxicity coefficient (CTC) = measured toxicity index / theoretical toxicity index × 100

[0056] Co-toxicity coefficient classification: When CTC is greater than 120, the mixture has synergistic effects; when CTC is less than 80, it is antagonistic; and when CTC is between 80 and 120, it is additive.

[0057] Table 1 The results of toxicity test of different ratios of thiophanate-methyl, thiophanate-methyl and metalaxyl against peanut root rot

[0058]

[0059]

[0060] From the test results in Table 1, it can be seen that the EC values ​​of thiophanate-methyl, thiophanate-methyl and metalaxyl-M on peanut root rot are 50 They are 57.14 mg / L, 20.36 mg / L and 11.19 mg / L respectively. After mixing according to the effective ingredient mass ratio of 1-4:1-4:1-5, they all have good bactericidal effects on peanut root rot, and the co-toxicity coefficient (CTC) is greater than 80, which has obvious synergistic and synergistic effects. Furthermore, when the weight ratio of the three is 1-4:1-4:1-5 (excluding 1:1:1), there is a synergistic and synergistic effect. When the ratio between the three is 2:1:1, the co-toxicity coefficient is 169.41, and the synergistic effect is the best. When the three active ingredients are compounded in pairs, their co-toxicity coefficients (CTC) are between 80 and 120, which has an additive effect.

[0061] Example 2: Screening of compound ratios of thiophanate-methyl, thiophanate-methyl and metalaxyl-M for peanuts and determination of co-toxicity coefficient 1. Experimental conditions

[0062] 1.1 Test targets

[0063] Peanut white rot (Sclerotium rolfsii sacc) was collected and isolated from Zhongmou County, Henan Province and stored at room temperature.

[0064] 1.2 Culture conditions

[0065] Before the assay, the bacteria were inoculated on PSA medium plates and pre-cultured at 25°C for 3 days. Mycelium blocks with a diameter of 5 mm were taken from the edge of the colony for assay.

[0066] 1.3 Instruments and Equipment

[0067] Biochemical incubator, SW-CJ-2F double-sided clean bench, eppendorf pipette, oven, hole puncher, culture dish, etc.

[0068] 2. Experimental Design

[0069] 2.1 Test Agents

[0070] 98% thiophanate-methyl technical (Shandong United Pesticide Industry Co., Ltd.), 95% thiophanate-methyl technical (Shaanxi Xida Huate Technology Industry Co., Ltd.), 92% metalaxyl-M technical (Antao Microbiology (Jiangsu) Co., Ltd.).

[0071] 2.2 Experimental treatment

[0072] 2.2.1 Dose setting

[0073] The test drugs thiofuranamide, thiophanate-methyl and metalaxyl-M were prepared into mother solutions with dimethyl sulfoxide and diluted with 2% Tween 80 sterile water. Then thiofuranamide, thiophanate-methyl and metalaxyl-M were compounded in 15 ratios of 4:1:5, 4:1:3, 4:1:1, 2:1:5, 2:1:3, 2:1:1, 1:1:5, 1:1:3, 1:1:1, 1:2:5, 1:2:3, 1:2:1, 1:4:5, 1:4:3, 1:4:1, etc. After the drugs were preparatory tested, the above-designed series of ratios were added to the PSA culture medium to prepare drug-containing plates, and the culture medium without drugs was set as a blank control (sterile water containing 2% Tween 80 instead of water).

[0074] 2.2.2 Experimental Repetition

[0075] Each concentration treatment was repeated 4 times.

[0076] 3. Test methods

[0077] The mycelium growth rate method was used. The pre-prepared mycelium blocks were picked and inoculated on PSA medium plates with different drug concentrations and cultured at 25°C for 4 days. The colony diameter was measured by the cross method, the average colony diameter was calculated, and the EC50 of different drugs on the strain was determined. The co-toxicity coefficient (CTC) of the mixture was calculated by the co-toxicity coefficient calculation method. The synergistic effect of the mixture was determined, and the specific calculation method was as follows:

[0078] Take a single agent in the mixture as the standard agent (usually the one with the lower EC 50) and calculate:

[0079] Single-dose toxicity index = standard drug EC 50 / a single-dose EC 50×100

[0080] Measured toxicity index (ATI) = EC50 value of standard single agent / EC50 value of mixed agent × 100

[0081] Theoretical toxicity index (TTI) = toxicity index of single agent A × the proportion of single agent A in the mixture + toxicity index of single agent B × the proportion of single agent B in the mixture + toxicity index of single agent C × the proportion of single agent C in the mixture

[0082] Co-toxicity coefficient (CTC) = measured toxicity index / theoretical toxicity index × 100

[0083] Co-toxicity coefficient classification: When CTC is greater than 120, the mixture has synergistic effects; when CTC is less than 80, it is antagonistic; and when CTC is between 80 and 120, it is additive.

[0084] Table 2 The results of toxicity test of different ratios of thiophanate-methyl, thiophanate-methyl and metalaxyl against peanut white rot

[0085]

[0086] From the test results in Table 2, it can be seen that the EC values ​​of thiophanate-methyl, thiophanate-methyl and metalaxyl against peanut white rot are 50 They are 0.026mg / L, 3.432mg / L and 0.039mg / L respectively. After being mixed in the effective ingredient mass ratio of 1-4:1-4:1-5, they all have good bactericidal effects on peanut white rot, and the co-toxicity coefficient (CTC) is greater than 80, which has an additive and synergistic effect. Furthermore, when the weight ratio of the three is 4:1:3-5, 2:1:1-3, 1:1:5, and 1:2-4:3-5, they have a synergistic effect. When the ratio between the three is 2:1:1, the co-toxicity coefficient is 156.75, and the synergistic effect is the best. When the three active ingredients are compounded in pairs, their co-toxicity coefficients (CTC) are between 80 and 120, which has an additive effect.

[0087] 2. Preparation Examples

[0088] Preparation Example 1: (10+5+5)% Thiothiocarb·Thiophanate-methyl·Metalaxyl-M suspension seed coating agent

[0089] The specific formula of the suspended seed coating agent is as follows:

[0090]

[0091] The preparation method is as follows: the active ingredients, auxiliary ingredients other than the film-forming agent and colorant, and deionized water are accurately weighed according to the ratio, and then homogenized and dispersed in a high-speed shear emulsifier disperser. After 30 minutes, zirconium beads with a weight ratio of 1:1.3 are added for sand grinding for 1.5 hours, and the sample is collected and filtered. Finally, the film-forming agent and colorant are added thereto for homogenized stirring, and the material is discharged after mixing evenly. Preparation Example 2: (10+5+5)% Thiofluzamide·Thiophanate-methyl·Metalaxyl-M suspended seed coating agent. The specific formula of the suspended seed coating agent is as follows:

[0092]

[0093] Preparation method: Same as Preparation Example 1.

[0094] Preparation Example 3: (10+5+5)% Thiothiocarb·Thiophanate-methyl·Metalaxyl-M suspended seed coating agent The specific formula of the suspended seed coating agent is as follows:

[0095]

[0096]

[0097] Preparation method: Same as Preparation Example 1.

[0098] Preparation Example 4: (10+5+5)% Thiothiocarb·Thiophanate-methyl·Metalaxyl-M suspended seed coating agent The specific formula of the suspended seed coating agent is as follows:

[0099]

[0100]

[0101] Preparation method: Same as Preparation Example 1.

[0102] Preparation Example 5: (10+5+5)% Thiothiocarb·Thiophanate-methyl·Metalaxyl-M suspended seed coating agent The specific formula of the suspended seed coating agent is as follows:

[0103]

[0104] Preparation method: Same as Preparation Example 1.

[0105] Preparation Example 6: (10+5+5)% Thiothiocarb·Thiophanate-methyl·Metalaxyl-M suspended seed coating agent The specific formula of the suspended seed coating agent is as follows:

[0106]

[0107] Preparation method: Same as Preparation Example 1.

[0108] Preparation Example 7: (10+5+5)% Thiothiocarb·Thiophanate-methyl·Metalaxyl-M suspended seed coating agent The specific formula of the suspended seed coating agent is as follows:

[0109]

[0110]

[0111] Preparation method: Same as Preparation Example 1.

[0112] Comparative Example 1: 25% thiothiocarb suspension seed coating agent

[0113]

[0114] According to the above-mentioned Preparation Examples 1 to 7, the sample preparation of (10+5+5)% thiophanate-methyl·thiophanate-methyl·metalaxyl-M FS was completed. The storage stability of each preparation example under four conditions of cold storage, hot storage, room temperature and freeze-thaw was observed through a 14-day accelerated storage test. The final most stable formula was selected according to the stability conditions to carry out field efficacy tests.

[0115] The specific observations are shown in Table 3:

[0116] Table 3 Statistics of observation results after sample preparation of different preparation examples

[0117]

[0118] By observing and testing the various indicators of the samples of Preparation Examples 1 to 7, it was found that Preparation Example 3 was superior to the other 6 preparation examples in terms of water dispersibility, particle size, pH, coating adhesion rate, and stability in cold storage, hot storage, room temperature, and freeze-thaw after 14 days of accelerated storage, and the content of the active ingredient did not decompose at all when tested at expiration, and was normal. Therefore, through laboratory sample preparation and stability observation, Preparation Example 3 was finally selected as the final stable formula, and later field efficacy tests can be carried out.

[0119] 3. Field efficacy test

[0120] Through the above indoor toxicity test of thiophanate-methyl, thiophanate-methyl and metalaxyl-M against peanut root rot and peanut white rot, the best ratio among the three agents was screened out to be 2:1:1, so this ratio was selected for the final stable formula screening in the laboratory. Through laboratory sample preparation observation and testing of various indicators, the best stable formula was finally screened out as Preparation Example 3 for subsequent field efficacy tests.

[0121] Test Example 1: Field test of the efficacy of thiophanate-methyl, thiophanate-methyl and metalaxyl-M against peanut root rot 1.1 Test agents and treatments

[0122] Preparation Example 3: 75g / 100kg seeds (preparation dosage / mu) for seed dressing;

[0123] Comparative Example 1: 80 g / 100 kg seeds (prepared dosage / mu) for seed dressing;

[0124] Commercially available control agent: 5% thiophanate-methyl suspension (Shaanxi Xida Huate Technology Experiment Co., Ltd.), 95g / 100kg seeds (prepared dosage / mu) for seed dressing;

[0125] Commercially available control agent: 20% metalaxyl-M suspension seed dressing agent (Jilin Bada Pesticide Co., Ltd.), 75g / 100kg seeds (prepared dosage / mu) for seed dressing;

[0126] Clear water control (CK).

[0127] 1.2 Test methods

[0128] Peanut variety tested: Yuhua 9327

[0129] Selection of experimental site: The experimental site is located in Zhengyang, Henan Province. The soil of the experimental field is loam with medium to high fertility. The previous crop is also peanuts, and it is fallow in winter.

[0130] Experimental design: The experiment set up 5 treatments: 20% thiophanate-methyl·thiophanate-methyl·metalaxyl-M FS, 25% thiophanate-methyl FS, 5% thiophanate-methyl SC, 20% metalaxyl-M FS and clear water control (CK). The drug-seed ratio adopted the drug treatment method mentioned in 1.1 above, and the seeds were mixed once 3 days before sowing. The application amount was strictly in accordance with the commonly used concentration or dosage of various drugs. For each treatment, 4 kg of peanut seeds were weighed and placed in a clean plastic pot for seed mixing. The seeds were evenly covered with the drug. They were dried and sown. Random block arrangement was used, with 4 replications, 6 rows in each plot, and the plot area was 24m 2 (row spacing 0.4m, row length 10.0m). 750kg / hm2 of compound fertilizer can be spread before sowing. 2 , the sowing rate is 15,000 holes / hm2 2 Other field management measures are the same as those for general high-yield peanut fields.

[0131] 1.3 Survey Methods

[0132] Seedling rate survey: Seed dressing was carried out on April 21, 2022, and sowing was completed on April 23. After sowing, the emergence of each treatment was regularly observed and recorded; the peanut emergence rate was counted on May 8 to evaluate the effect of the pesticide on the peanut emergence rate.

[0133] Investigation of control effect: At the seedling stage, flowering stage and spore fruit maturity stage, a "Z"-shaped five-point sampling method was used, with 10 plants at each point, to investigate the occurrence of peanut root rot in the field, count the number of diseased plants, and calculate the incidence rate and control effect. The classification standards for peanut root rot are as follows:

[0134] Level 0: There are no lesions on the stem base and main fibrous roots;

[0135] Level 1: There are a few lesions on the stem base and taproot;

[0136] Level 3: There are many lesions on the stem base and main root, and the area of ​​lesions accounts for 1 / 4 to 1 / 2 of the total area of ​​the stem base and root;

[0137] Level 5: There are many large lesions on the stem base and main root, and the area of ​​lesions accounts for 1 / 2 to 3 / 4 of the total area of ​​the stem base and root;

[0138] Level 7: The lesions on the stem base and main root are connected, forming a stem-wrapped phenomenon, but the root system is not dead;

[0139] Level 9: Root necrosis and aboveground parts of plants wilt or die.

[0140] According to the relevant standards of national field trials, the disease index and control effect were calculated after statistical records of diseased plants were made, and the Duncan's new multiple range (DMRT) method was used to conduct significance analysis on the control effect.

[0141] Seedling emergence rate (%) = number of treated seedlings / total number of surveys × 100

[0142] Disease rate (%) = (number of diseased plants / total number of plants surveyed) × 100

[0143] Disease index = 100 × ∑ (number of diseased plants at each level × relative level value) / (highest disease level × total number of plants surveyed)

[0144] Control effect (%) = (disease index of control area - disease index of drug-treated area) / disease index of control area × 100

[0145] When peanuts were harvested, the pod yield of each plot was measured, the actual yield per hectare was calculated, and the yield increase rate was calculated.

[0146] Yield increase rate (%) = (pod yield in treatment area - pod yield in control area) / pod yield in control area × 100 Table 4 Effect of each treatment on peanut field emergence rate and peanut root rot

[0147]

[0148] Table 5 Effects of each treatment on peanut yield

[0149]

[0150] Note: The data in the table are the average values ​​of 4 repetitions, and the following lowercase letters indicate significant differences (P < 0.05), and uppercase letters indicate significant differences (P < 0.01).

[0151] By observing Table 4 and Table 5, it can be concluded that the combination of thiophanate-methyl, thiophanate-methyl and metalaxyl-M has the best control effect and yield-increasing effect on peanut root rot when the ratio is 2:1:1, with a control effect of 91.38% and a yield-increasing rate of 9.89%, which is significantly better than other treatment groups. In addition, while reducing the diseased plant rate, it also helps to increase the peanut germination rate by 95.64%. This comprehensively shows that the reasonable combination of the three compounds allows the combination to have a good synergistic effect. Secondly, no pesticide damage was found during the entire application process, so farmers can use it with confidence.

[0152] Test Example 2: Field test of the efficacy of thiophanate-methyl, thiophanate-methyl and metalaxyl-M against peanut white rot 2.1 Test agents and treatments

[0153] Preparation Example 3: 75g / 100kg seeds (preparation dosage / mu) for seed dressing;

[0154] Comparative Example 1: 80 g / 100 kg seeds (prepared dosage / mu) for seed dressing;

[0155] Commercially available control agent: 5% thiophanate-methyl suspension (Shaanxi Xida Huate Technology Experiment Co., Ltd.), 95g / 100kg seeds (prepared dosage / mu) for seed dressing;

[0156] Commercially available control agent: 20% metalaxyl-M suspension seed dressing agent (Jilin Bada Pesticide Co., Ltd.), 75g / 100kg seeds (prepared dosage / mu) for seed dressing;

[0157] Clear water control (CK).

[0158] 2.2 Test methods

[0159] Peanut variety tested: Yuanza 9847

[0160] Test site: Shandong Tai'an, the test site is a continuous peanut crop field, and peanut white rot has occurred seriously for many years. The peanut planting method is the same, artificial ridge, ridge width 55cm, ridge height 20cm, single ridge double row hole, hole distance 20cm, 2 seeds per hole. The soil, cultivation and water and fertilizer management conditions of each test area are the same.

[0161] Experimental design: The experiment set up 5 treatments: 20% thiophanate-methyl·thiophanate-methyl·metalaxyl-M FS, 25% thiophanate-methyl FS, 5% thiophanate-methyl SC, 20% metalaxyl-M FS and clear water control (CK). The drug-seed ratio adopted the drug treatment method in 2.1 above, and the seeds were mixed once 3 days before sowing. The application amount was strictly in accordance with the commonly used concentration or dosage of various drugs. For each treatment, 5 kg of peanut seeds were weighed and placed in a clean plastic pot for seed mixing. The seeds were evenly covered with the drug. They were dried and sown. Random block arrangement was used, with 4 replicates for each treatment and a plot area of ​​30 m 2 .

[0162] 2.3 Survey Methods

[0163] Seedling rate survey: Seed dressing was carried out on April 15, 2022, and sowing was completed on April 17. After sowing, the emergence of each treatment was regularly observed and recorded; on May 2, the peanut emergence rate was counted, 5 points were randomly selected in each plot, and 25 holes were investigated at each point to evaluate the effect of the pesticide on the peanut emergence rate and observe the growth of peanuts.

[0164] Investigation of control effect: At the seedling stage, flowering stage and spore fruit maturity stage, a "Z"-shaped 5-point sampling method was used to investigate 30 plants in each plot to investigate the occurrence of white rot in the field, count the number of diseased plants, and calculate the incidence rate and control effect. The classification standards for white rot in peanuts are as follows:

[0165] Level 0: Plants are asymptomatic;

[0166] Level 1: Lesions only occur at the base of the stem;

[0167] Level 2: Constriction symptoms occur at the base of the stem, and less than 1 / 3 of the entire plant shows symptoms (wilt, death, wilt, etc.);

[0168] Level 3: Less than 2 / 3 of the plant shows symptoms;

[0169] Level 4: More than 2 / 3 of the plant shows symptoms.

[0170] According to the relevant standards of national field trials, the disease index and control effect were calculated after statistical records of diseased plants were made, and the Duncan's new multiple range (DMRT) method was used to conduct significance analysis on the control effect.

[0171] Seedling emergence rate (%) = number of treated seedlings / total number of surveys × 100

[0172] Disease rate (%) = (number of diseased plants / total number of plants surveyed) × 100

[0173] Disease index = 100 × ∑ (number of diseased plants at each level × relative level value) / (highest disease level × total number of plants surveyed)

[0174] Control effect (%) = (disease index of control area - disease index of drug-treated area) / disease index of control area × 100

[0175] When peanuts were harvested, the pod yield of each plot was measured, the actual yield per hectare was calculated, and the yield increase rate was calculated.

[0176] Yield increase rate (%) = (pod yield in treatment area - pod yield in control area) / pod yield in control area × 100 Table 6 Effect of each treatment on peanut field emergence rate and control of peanut white rot

[0177]

[0178] Table 7 Effects of each treatment on peanut yield

[0179]

[0180] Note: The data in the table are the average values ​​of 4 repetitions, and the following lowercase letters indicate significant differences (P < 0.05), and uppercase letters indicate significant differences (P < 0.01).

[0181] By observing Table 6 and Table 7, it can be concluded that the combination of thiophanate-methyl, thiophanate-methyl and metalaxyl-M has the best control effect and yield-increasing effect on peanut white rot when the ratio is 2:1:1, with a control effect of 93.85% and a yield-increasing rate of 7.35%, which is significantly better than other treatment groups. It can be found that the combined agent can effectively increase the emergence rate of peanuts by 93.86% while reducing the diseased plant rate. Therefore, the above analysis shows that the reasonable combination of the three compounds has a good synergistic effect. Secondly, no phytotoxicity occurs during the entire application process, and it is safe to use, so it can be used with confidence.

[0182] The indoor toxicity test and field test on the combination of the three active ingredients of thiophanate-methyl, thiophanate-methyl and metalaxyl-M for the prevention and control of peanut root rot and peanut white rot showed that the control effect of the combination of the three active ingredients was significantly better than that of the single-dose control agent, especially the seed dressing can effectively control the initial infection source of peanut root rot and white rot and the occurrence of some soil-borne diseases from the source, secondly, it is convenient for sowing, and it also has a very favorable protection and promotion effect on seed germination, which greatly improves production efficiency and reduces labor costs.

Claims

1. A suspension seed coating agent containing thiophanate-methyl, thiophanate-methyl and metalaxyl-M, characterized in that: The suspended seed coating agent consists of active ingredients and auxiliary ingredients, wherein the active ingredients are thiophanate-methyl, thiophanate-conazole and metalaxyl-M, wherein the mass ratio of thiophanate-methyl, thiophanate-conazole and metalaxyl-M is 1-5:1-5:1-10.

2. The suspension seed coating agent according to claim 1, characterized in that: The mass ratio of thiophanate-methyl, thiophanate-methyl and metalaxyl-M is 1-4:1-4:1-5.

3. The suspension seed coating agent according to claim 1, characterized in that: The auxiliary components include one or more of a dispersant, a wetting agent, a penetrant, an antifreeze agent, a thickener, a defoaming agent, a film former, a preservative, a colorant, a pH regulator, and deionized water.

4. The suspension seed coating agent according to claim 1, characterized in that: The formula of the suspended seed coating agent includes: 3% to 20% of active ingredients, 3.5% to 6% of dispersant, 2% to 5% of wetting agent, 0% to 4% of penetrant, 1% to 6% of antifreeze agent, 0.1% to 3% of thickener, 0.3% to 1% of defoaming agent, 0.5% to 3% of film-forming agent, 0.1% to 0.5% of preservative, 3% to 17% of colorant, 0% to 3% of pH regulator and 30% to 80% of deionized water.

5. A method for preparing the suspension seed coating agent according to any one of claims 1 to 4, characterized in that: The method comprises the following steps: firstly, the effective ingredients, auxiliary ingredients except the film-forming agent and the colorant, and deionized water are accurately weighed according to the ratio, and then homogenized and dispersed in a high-speed shear emulsifying disperser, after 30 minutes, zirconium beads are added for sand grinding for 1.5 hours, the sample is collected and filtered, and finally the film-forming agent and the colorant are added thereto for homogenized stirring, and the material is discharged after being evenly mixed to obtain the product.

6. Use of the suspended seed coating agent according to any one of claims 1 to 4 for coating and dressing peanut seeds before planting.

7. Use of the suspended seed coating agent according to any one of claims 1 to 4 for preventing and controlling fungal and bacterial diseases of crops.

8. The use according to claim 7, characterized in that The crop is preferably peanut.

9. The use according to claim 7, characterized in that: The diseases are preferably peanut root rot and peanut white rot.

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