Bactericide composition and application thereof

Abstract

The invention relates to the field of plant protection, and particularly discloses a bactericidal composition containing components (A) and (B) and application of the bactericidal composition. A component (A) in the bactericidal composition is a compound with a formula (I), component (B) is selected from the group consisting of respiration inhibitors, sterol biosynthesis inhibitors, nucleic acid synthesis inhibitors, cell division and cytoskeleton inhibitors, amino acid and protein synthesis inhibitors, signal transduction inhibitors, lipid and membrane synthesis inhibitors, inhibitors having a multi-site action, and cell wall synthesis inhibitors. The composition disclosed by the invention can be used for controlling or preventing plants from being infected by plant pathogenic microorganisms in agriculture or horticulture. # imgabs0 #

Description

Technical Field

[0001] The invention belongs to the field of plant protection, and in particular relates to a fungicide composition and application thereof. Background Art

[0002] Plant diseases pose a serious threat to global food security. At present, production mainly relies on chemical pesticides to control crop diseases, but the irrational use of chemical pesticides can easily lead to problems such as pest resistance and environmental pollution, which seriously threatens food production and environmental safety. In view of the problems of most fungicides, such as single structure, narrow fungicide spectrum, and low single-agent efficacy, reasonable compounding can significantly increase efficiency and reduce dosage, reduce environmental pollution, and at the same time delay the development of resistance and expand the prevention and control spectrum.

[0003] In particular, rice is one of the most widely planted and important food crops in the world, feeding more than half of the world's population. With the rapid increase in the global population, the demand and safety of rice production have become increasingly important. Rice blast caused by Magnaporthe oryzae is the most important and destructive fungal disease that occurs widely in rice-growing areas in my country and even the world, posing a serious threat to global food production security. At present, the prevention and control of this disease still focuses on breeding disease-resistant varieties and chemical control. Due to the complex field pathotypes of rice blast fungi and the rapid changes in population composition, disease-resistant varieties lose their disease resistance after 3-5 years of promotion. Chemical control is usually costly, and the resistance of pathogens continues to develop, resulting in limited control effects of current fungicides and easy environmental pollution.

[0004] Patents CN113683509A and CN114890899A disclose a class of diphenyl ether ester compounds of formula (II) and their preparation methods, and disclose their use in preventing and controlling rice blast. However, there are no reports on the composition of the diphenyl ether ester compounds of formula (II) specifically disclosed in patents CN113683509A and CN114890899A and other fungicides.

[0005]

[0006] Most pathogenic fungi or oomycetes rely on asexual conidia or zoospores to achieve infection and spread of diseases. If the spore germination of pathogens can be inhibited, the infection of plants by pathogenic microorganisms can be controlled or prevented. Summary of the invention

[0007] The object of the present invention is to provide compositions having excellent control efficacy against plant diseases. Synergistic combinations (synergistic combinations), including mixtures, comprising component (A) and at least one additional fungicide component (B) are provided. Another object of the present invention is to provide compositions and uses of these synergistic combinations (including mixtures).

[0008] The present invention provides a fungicidal combination, comprising:

[0009] Component (A) is a compound having formula (I):

[0010]

[0011] And component (B) is a compound selected from the group consisting of:

[0012] A) Respiratory depressants

[0013] - Complex III inhibitors at the Qo site: azoxystrobin, coumoxystrobin, dimoxystrobin, fenaminstrobin, flufenoxystrobin, fluoxastrobin, kresoxim-methyl, picoxystrobin, pyraclostrobin, pyrametostrobin, pyraoxystrobin, trifloxystrobin, triclopyricarb, famoxadone, metyltetraprole;

[0014] -Qi site complex III inhibitors: cyazofamid, amisulbrom, fenpicoxamid, florylpicoxamid, metarylpicoxamid;

[0015] - Complex II inhibitors: benzovindiflupyr, bixafen, boscalid, fluopyram, fluxapyroxad, furametpyr, isofetamid, isopyrazam, mepronil, penflufen, sedaxane, thifluzamide, flubeneteram, pydiflumetofen, pyraziflumid, fluindapyr, penthiopyrad, inpyrfluxam, isofluxuram, pyrapropoyne, cyclobutrifluram;

[0016] - Other respiratory inhibitors: fluazinam, ametoctradin and silthiofam;

[0017] Complex II inhibitors: diflumetorim;

[0018] B) Sterol biosynthesis inhibitors

[0019] -C14 demethylase inhibitors: Triazoles: azaconazole, bitertanol, bromuconazole, cyproconazole, difenoconazole, diniconazole, diniconazole-M, epoxiconazole, fenbuconazole, fluquinconazole, flusilazole, flutriafol, hexaconazole, imibenconazole, ipconazole, metconazole, myclobutanil, oxpoconazole, penconazole, propiconazole propiconazole, prothioconazole, simeconazole, tebuconazole, tetraconazole, triadimefon, triadimenol, triticonazole, uniconazole, fluoxytioconazole, ipfentrifluconazole, mefentrifluconazole; imidazoles: imazalil, pefurazoate, prochloraz, triflumizole; pyrimidines, pyridines and piperazines: fenarimol, nuarimol, pyrifenox, triforine;

[0020] -δ14-reductase inhibitors: aldimorph, dodemorph, dodemorph-acetate, fenpropimorph, tridemorph, fenpropidin, piperalin, spiroxamine;

[0021] -3-Ketoreductase inhibitors: fenhexamid;

[0022] C) Nucleic acid synthesis inhibitors

[0023] - Phenylamide or acylamino acid fungicides: benalaxyl, benalaxyl-M, metalaxyl, mefenoxam, ofurace, oxadixyl;

[0024] -Others: octhilinone, bupirimate;

[0025] D) Inhibitors of cell division and cytoskeleton

[0026] - Tubulin inhibitors: benomyl, carbendazim, fuberidazole, thiabendazole, thiophanate-methyl;

[0027] - Other cell division inhibitors: diethofencarb, ethaboxam, pencycuron, fluopicolide, zoxamide, metrafenone, pyriofenone;

[0028] E) Amino Acids and Protein Synthesis Inhibitors

[0029] - Methionine synthesis inhibitors (anilinopyrimidines): cyprodinil, mepanipyrim, pyrimethanil;

[0030] - Protein synthesis inhibitors: blasticidin-S, kasugamycin, kasugamycin hydrochloride-hydrate, streptomycin, oxytetracycline, validamycin A;

[0031] F) Signal transduction inhibitors

[0032] - MAP / histidine protein kinase inhibitors: fluoroimide, iprodione, procymidone, vinclozolin, fenpiclonil, fludioxonil;

[0033] - G protein inhibitor: quinoxyfen;

[0034] G) Lipid and membrane synthesis inhibitors

[0035] - Phospholipid biosynthesis inhibitors: edifenphos, iprobenfos, pyrazophos, isoprothiolane;

[0036] - Lipid peroxidation: dicloran, quintozene, tecnazene, tolclofos-methyl, chloroneb, etridiazole;

[0037] - Phospholipid biosynthesis and cell wall deposition: dimethomorph, flumorph, mandipropamid, benthiavalicarb, iprovalicarb, valifenalate;

[0038] - Compounds and fatty acids that affect cell membrane permeability: propamocarb, propamocarb-hydrochlorid;

[0039] - Oxysterol-binding protein (OSBP) inhibitors: oxathiapiprolin, fluoxapiprolin;

[0040] H) Inhibitors with multi-site action

[0041] - Inorganic active substances: Bordeaux mixture, copper acetate, copper hydroxide, copper oxychloride, basic copper sulfate, sulfur;

[0042] -Thio- and dithiocarbamates: ferbam, mancozeb, maneb, metam, metiram, propineb, thiram, zineb, ziram;

[0043] - Organochlorine compounds: anilazine, chlorothalonil, captafol, captan, folpet, dichlofluanid, dichlorophen, flusulfamide, hexachlorobenzene, phthalide, tolylfluanid;

[0044] - Guanidines and others: dodine, guazatine, guazatine acetate, iminoctadine, iminoctadine-triacetate, iminoctadine-tris(albesilate), dithianon;

[0045] I) Cell wall synthesis inhibitors

[0046] - Glucan synthesis inhibitors: validamycin, polyoxin D; melanin synthesis inhibitors: pyroquilon, tricyclazole, carpropamid, fenoxanil.

[0047] Preferably, component (B) is a compound selected from the group consisting of:

[0048] The complex III inhibitor at the Qo site is selected from kresoxim-methyl, azoxystrobin, pyraclostrobin or trifloxystrobin;

[0049] The complex II inhibitor is selected from pydiflumetofen, boscalid or fluopyram;

[0050] The complex I inhibitor is selected from diflumetorim;

[0051] Other respiratory depressants are selected from fluazinam;

[0052] The C14 demethylase inhibitor is selected from difenoconazole, tebuconazole, metconazole, prothioconazole or prochloraz;

[0053] The nucleic acid synthesis inhibitor is selected from metalaxyl or metalaxyl-M (mefenoxam);

[0054] The microtubule inhibitor is selected from carbendazim, thiophanate-methyl or thiabendazole;

[0055] The protein synthesis inhibitor is selected from kasugamycin, kasugamycin hydrochloride-hydrate or validamycin A;

[0056] The signal transduction inhibitor is selected from procymidone or fludioxonil;

[0057] The lipid and membrane synthesis inhibitor is selected from dimethomorph, isoprothiolane or iprobenfos;

[0058] The inhibitor with multi-site action is selected from Bordeaux mixture, sulfur, mancozeb or chlorothalonil;

[0059] The melanin synthesis inhibitor is selected from tricyclazole or fenoxanil.

[0060] More preferably, component (B) is a compound selected from the group consisting of:

[0061] The complex III inhibitor at the Qo site is selected from azoxystrobin, pyraclostrobin or trifloxystrobin;

[0062] The complex II inhibitor is selected from pydiflumetofen;

[0063] The complex I inhibitor is selected from diflumetorim;

[0064] Other respiratory depressants are selected from fluazinam;

[0065] The C14 demethylase inhibitor is selected from difenoconazole, tebuconazole or prochloraz;

[0066] The protein synthesis inhibitor is selected from kasugamycin, kasugamycin hydrochloride-hydrate or validamycin A;

[0067] The microtubule inhibitor is selected from thiophanate-methyl or thiabendazole;

[0068] The signal transduction inhibitor is selected from procymidone;

[0069] The lipid and membrane synthesis inhibitor is selected from dimethomorph, isoprothiolane or iprobenfos;

[0070] The melanin synthesis inhibitor is selected from tricyclazole or fenoxanil.

[0071] When the composition of the present invention is a two-component composition, the weight ratio of component (A) to component (B) is 400:1 to 1:50.

[0072] Preferably, the weight ratio of component (A) to component (B) is from 200:1 to 1:10.

[0073] More preferably, the weight ratio of component (A) to component (B) is from 20:1 to 1:5.

[0074] The present invention also provides a mixture or composition comprising the combination described herein, which further comprises at least one auxiliary agent selected from solvents, fillers and carriers, emulsifiers, dispersants, wetting agents, spreaders, stickers, stabilizers or synergists.

[0075] The composition of the present invention may be a mixture of component (A) and component (B) of the present invention itself, and is usually prepared by mixing the compound of component (A) of the present invention, component (B) of the present invention and an inert carrier, and optionally adding surfactants and other adjuvants for formulation.

[0076] The composition of the present invention can be formulated into oil solution, emulsifiable concentrate, flowable preparation, wettable powder, water dispersible granule, dust or granule. Such preparation can be used directly as a plant disease control agent or after adding other inert ingredients.

[0077] In the composition of the present invention, the total amount of component (A) and component (B) of the present invention is generally in the range of 0.1 to 99 wt%, preferably 0.2 to 90 wt%, and more preferably 1 to 80 wt%.

[0078] Examples of the solid carrier used in the preparation include the following in the form of fine powder or particulates: clay (e.g., kaolin, diatomaceous earth, synthetic hydrous silica, Fubasami clay, bentonite and acid clay), talc or other inorganic minerals (e.g., sericite, quartz powder, sulfur powder, activated carbon, calcium carbonate and hydrous silica), and examples of the liquid carrier include: water, alcohols (e.g., methanol and ethanol), ketones (e.g., acetone and methyl ethyl ketone), aromatic hydrocarbons (e.g., benzene, toluene, xylene, ethylbenzene and methylnaphthalene), aliphatic hydrocarbons (e.g., n-hexane, cyclohexane and kerosene), esters (e.g., ethyl acetate and butyl acetate), nitriles (e.g., acetonitrile and isobutyronitrile), ethers (e.g., dioxane and diisopropyl ether), amides (e.g., DMF and dimethylacetamide), halogenated hydrocarbons (e.g., ethylene dichloride, trichloroethylene and carbon tetrachloride) and the like.

[0079] Examples of the surfactant include alkyl sulfates, alkyl sulfonates, aryl alkyl sulfonates, alkyl aryl ethers and polyoxyethylated compounds thereof, polyethylene glycol ethers, polyhydric alcohol esters and sugar alcohol derivatives.

[0080] Examples of other formulation adjuvants include adhesives, dispersants and stabilizers, and specific examples include: casein, gelatin, polysaccharides (e.g., starch, gum arabic, cellulose derivatives and alginic acid), lignin derivatives, bentonite, sugars, water-soluble synthetic polymers (e.g., polyvinyl alcohol, polyvinyl pyrrolidone and polyacrylic acid), PAP (isopropyl acid phosphate), BHT (2,6-di-tert-butyl-4-methylphenol), BHA (a mixture of 2-tert-butyl-4-methoxyphenol and 3-tert-butyl-4-methoxyphenol), vegetable oils, mineral oils, fatty acids or their fatty acid esters, etc.

[0081] The composition of the present invention can also be formulated in the following manner: each of the component A compound of the present invention and the component B compound of the present invention is formulated according to the above-mentioned method, and when necessary, it is diluted with water to obtain a preparation containing the component A compound of the present invention or a diluted solution containing it, or a preparation containing the component B compound of the present invention or a diluted solution containing it, and then the obtained preparations or diluted solutions are mixed with each other.

[0082] The concentrated formulations may be dispersed in water or other liquids for application, or the formulation may be a dust or granules which are ready for application without further treatment. The formulations may be prepared according to methods common in the agrochemical art, but are novel and important due to the presence of the composition in the formulation.

[0083] The most commonly used formulations are aqueous suspensions or emulsions. The water-soluble, water-suspendable or emulsifiable formulations are usually referred to as wettable powders when prepared as solids, or as emulsifiable concentrates, aqueous suspensions or suspension concentrates when the water-soluble, water-suspendable or emulsifiable formulations are liquids. The present invention contemplates the use of all vehicles by which the composition can be formulated for delivery and use as a bactericide.

[0084] It is readily understood that any substance to which these compositions can be added can be used provided that they can achieve the desired efficacy without significantly interfering with the activity of these compositions as crop antifungal agents. Wettable powders that can be pressed into water-dispersible granules contain an intimate mixture of the composition, a carrier, and an agricultural surfactant. The concentration of the composition in the wettable powder is generally about 10% to about 90% by weight, more preferably about 25% to about 75% by weight, based on the total weight of the formulation. In the preparation of wettable powder formulations, the composition can be mixed with any finely pulverized solid, such as prophyllite, talc, chalk, gypsum, Fuller's earth, bentonite, attapulgite, starch, casein, gluten, montmorillonite clay, diatomaceous earth, purified silicate, etc. In such operation, a finely divided carrier is usually ground and mixed with the composition in a volatile organic solvent. Effective surfactants, which comprise from about 0.5% to about 10% by weight of the wettable powder, include sulfonated lignin, naphthalene sulfonates, alkylbenzene sulfonates, alkyl sulfates, and nonionic surfactants such as ethylene oxide adducts of alkylphenols.

[0085] The emulsifiable concentrate of the composition is contained in a conventional concentration in a suitable liquid, for example from about 10% by weight to about 50% by weight, based on the gross weight of the emulsifiable concentrate formulation. The components of the composition are dissolved in a carrier, either jointly or separately, which is a mixture of a water-miscible solvent or a water-immiscible organic solvent and an emulsifier. The emulsifiable concentrate can be diluted with water and oil to form a spray mixture in the form of an oil-in-water emulsion. Useful organic solvents include aromatic parts of petroleum, particularly high-boiling cycloalkane parts and alkene parts such as heavy aromatic naphtha. Other organic solvents such as terpene solvents may also be used, including rosin derivatives, aliphatic ketones such as cyclohexanone and complex alcohols such as 2-ethoxyethanol.

[0086] The emulsifier that can be advantageously adopted by the present invention can be easily determined by those skilled in the art and includes various nonionic emulsifiers, anionic emulsifiers, cationic emulsifiers and amphoteric emulsifiers, or a blend of two or more emulsifiers. The example of the nonionic emulsifier used in the preparation of the emulsifiable concentrate includes polyalkylene glycol ethers, and condensation products of alkyl and aryl phenols, aliphatic alcohols, aliphatic amines or fatty acids with ethylene oxide, propylene oxide, such as ethoxylated alkylphenols and carboxylates dissolved with polyols or polyoxyalkylenes. Cationic emulsifiers include quaternary ammonium compounds and fatty amine salts. Anionic emulsifiers include oil-soluble salts (e.g., calcium salts), oil-soluble salts or suitable salts of sulfated polyglycol ethers and phosphorylated polyglycol ethers of alkylaryl sulfonic acids.

[0087] Representative organic liquids that can be used in the preparation of the emulsifiable concentrate of the present invention are aromatic liquids such as xylene and propylbenzene fractions; or mixed naphthalene fractions, mineral oils, substituted aromatic organic liquids such as dioctyl phthalate; kerosene; dialkylamides of various fatty acids, particularly dimethylamides of fatty glycols and glycol derivatives such as n-butyl ether, ethyl ether or methyl ether of diethylene glycol, and methyl ether of triethylene glycol. A mixture of two or more organic liquids can also be used in the preparation of emulsifiable concentrates. Preferred organic liquids are xylene and propylbenzene fractions, of which xylene is the most preferred. Surfactant dispersants are generally used in liquid formulations, and the amount of the surfactant dispersant is generally 0.1% to 20% by weight based on the combined weight of the dispersant and the composition. The formulation may also contain other compatible additives, such as plant growth regulators and other biologically active compounds used in agriculture.

[0088] Aqueous suspensions include suspensions in which one or more water-insoluble compounds are dispersed in an aqueous vehicle, with a concentration of about 5% by weight to about 70% by weight based on the total weight of the aqueous suspension formulation. The suspension is prepared as follows: the components of the composition are finely ground together or separately, and the ground material is violently mixed into a vehicle, the vehicle being composed of water and a surfactant selected from the same type of discussion above. Other ingredients, such as inorganic salts and synthetic or natural gums, may also be added to increase the density and viscosity of the aqueous vehicle. It is usually most effective to grind and mix simultaneously in the following manner: prepare an aqueous mixture and homogenize in an equipment such as a sand mill, a ball mill or a piston homogenizer.

[0089] Composition can also be used as the form of granular preparation, and described granular preparation is particularly used for being applied to soil.In the gross weight of granular preparation, described granular preparation contains about 0.5 weight % to about 10 weight % described compound dispersed in carrier usually, and described carrier is made up of attapulgite, bentonite, diatomite, clay or similar cheap material of coarse crushing completely or mostly.Described preparation is prepared as follows usually: described composition is dissolved in suitable solvent, is applied to granular carrier, and described granular carrier has been made into suitable particle size (scope is about 0.5 to about 3mm) in advance.Described preparation can also be prepared as follows: described carrier and composition are made into pasty or paste, then crush and dry and obtain required particle size.

[0090] Dusts containing the composition are simply prepared by homogenizing the powdered composition and a suitable dusty agricultural carrier (e.g., kaolin clay, ground volcanic rock, etc.) The dust may suitably contain from about 1% to about 10% by weight of the composition / carrier combination.

[0091] The preparation may contain agricultural auxiliary surfactants to enhance the deposition, wetting and penetration of the composition onto target crops and microorganisms. These auxiliary surfactants may be optionally used as components of the preparation or as a barrel mixture. In terms of the spray volume of water, the amount of the auxiliary surfactant will be 0.01 volume / volume (v / v) % to 1.0 v / v%, preferably 0.05 v / v % to 0.5 v / v %. Suitable auxiliary surfactants include ethoxylated nonyl acid, ethoxylated synthetic alcohol or ethoxylated natural alcohol, sulfosuccinate or sulfosuccinate, ethoxylated organosiloxane, ethoxylated fatty amines, and blends of surfactants and mineral oils or vegetable oils.

[0092] In some cases, it will be beneficial to spray the formulation of the composition of the present invention using an airplane or helicopter by aerial application. The exact components of these aerial applications depend on the crops being treated. Aerial applications of cereals utilize a spray volume of preferably 15L / ha to 25L / ha and standard diffusion or penetration type adjuvants, such as nonionic surfactants or crop oil concentrates, preferably 0.05% to 15%, based on the water of the spray volume. Aerial applications for fruiting crops (e.g., bananas) can utilize lower application rates and higher adjuvant concentrations, preferably in the form of adhesive adjuvants, such as fatty acids, latex, fatty alcohols, crop oils, and inorganic oils. The typical spray volume of fruiting crops is preferably 15L / ha to 30L / ha, and adjuvant concentrations reach up to 30%, based on the water of the spray volume. Typical examples may include, but are not limited to, an application volume of 23L / ha, with a 30% paraffin oil adhesive adjuvant concentration (e.g., Spraytex CT).

[0093] The present application includes within its scope a method for preventing or controlling or preventing plant pathogenic microorganisms from attacking. The method includes applying a composition of a bactericidal effective amount to the locus of the plant pathogenic microorganism or to the locus where infection (infestation) is to be prevented (e.g., applied to wheat or barley plants). The composition is suitable for treating various plants at an antimicrobial level while showing low phytotoxicity. The composition is used in the form of a protectant or eradicant. The composition is applied by any of a variety of known techniques, or as a composition or as a preparation comprising the composition. For example, the composition can be applied to the roots, seeds or leaves of the plant for preventing and controlling various plant pathogenic microorganisms without compromising the commercial value of the plant. The application form of the composition can be any of the commonly used formulation types, for example, as a solution, pulvis, wettable powder, flowable concentrate, or emulsifiable concentrate. These substances can be conveniently applied in various known ways.

[0094] The present invention also provides a method for treating plants to resist infection by pathogenic microorganisms, the method comprising applying a certain amount of component (A) and a certain amount of at least one component (B) to the plant, thereby treating the plant to resist infection by pathogenic microorganisms, wherein the component (A) and the component (B) are as defined above.

[0095] The present invention also provides a method for controlling plant diseases caused by pathogenic microorganisms, the method comprising applying an effective amount of a composition of component (A) and an effective amount of at least one component (B) to the pathogenic microorganisms and their habitats or locations thereof, thereby controlling the plant diseases, wherein the components (A) and (B) are as defined above.

[0096] The present invention also provides a method for controlling fungal attack on plants, seeds or seedlings, the method comprising applying the combination, mixture or composition described herein to the plants, seeds, seedlings, thereby controlling fungal attack on the plants, seeds or seedlings.

[0097] The present invention also provides a method for protecting plant propagation material, which method comprises treating the plant propagation material with a composition of an effective amount of component (A) and an effective amount of at least one component (B), wherein components (A) and (B) are as defined above.

[0098] Preferably, the plant or its propagation material is a transgenic plant or its seeds, or a plant or its seeds treated with genome editing technology.

[0099] Transgenic plants are plants whose genetic material has been modified by using recombinant DNA technology. Typically, one or more genes have been incorporated into the genetic material of such plants to improve certain characteristics of the plant.

[0100] The present invention also provides a use of a composition in controlling or inhibiting plant pathogenic microorganisms, preventing infection by plant pathogenic microorganisms, or protecting plant materials from infection and destruction by pathogenic microorganisms.

[0101] The composition of the present invention can be used to protect plants from plant diseases.

[0102] The control method of the present invention can control plant diseases by applying the composition of the present invention to plants or soil for cultivating plants, alternatively, separately applying component (A) of the present invention or component (B) of the present invention to plants or soil for cultivating plants.

[0103] There is no particular limitation on the method for applying the composition of the present invention as long as the application form is a form by which the compound of the present invention can be substantially applied, and includes, for example, application to plants such as foliar application; application to areas for cultivating plants such as immersion application; and application to seeds such as seed disinfection.

[0104] The application dosage of the composition of the present invention varies depending on weather conditions, dosage form, application timing, application method, area to be applied, target disease, target crop, etc., and is generally 1 to 500g, and preferably 2 to 200g / 1,000m2 to be applied area. Emulsifiable concentrates, wettable powders or suspensions, etc. are generally applied by diluting them with water. In this case, the concentration of the composition of the present invention after dilution is generally 0.0005 to 2% by weight, and preferably 0.005 to 1% by weight, and powders or granules, etc. are generally applied as is without diluting them. In seed application, the amount of the composition of the present invention is generally 0.001 to 100g, and preferably 0.01 to 50g / 1kg of seeds.

[0105] Examples of places where plant diseases grow include paddy fields, fields, tea gardens, orchards, non-agricultural land, houses, nursery trays, nursery boxes, nursery soil and seedbeds.

[0106] The composition of the present invention can be used as an agent for controlling plant diseases in agricultural land such as fields, rice fields, lawns and orchards. The composition of the present invention can control diseases occurring in agricultural land or other land used for cultivating the following "plants" and the like.

[0107] Plants and plant cultivars treated with the compositions of the invention include plants and plant cultivars that are hybrid plants that already express the characteristic of hybrid vigor or hybrid vigour, which generally results in greater yield, vigour, health and resistance to biotic and abiotic stresses.

[0108] The composition of the present invention can be advantageously used to treat transgenic plants, plant cultivars or plant parts that have received genetic material that imparts favorable and / or useful properties (traits) to these plants, plant cultivars or plant parts. Therefore, it is contemplated that the present invention is combined with one or more recombinant traits or transgenic events or combinations thereof. For the purposes of this application, transgenic events are produced by inserting specific recombinant DNA molecules into specific locations (sites) of plant genome chromosomes. The insertion creates a new DNA sequence that is referred to as an "event," characterized in that the inserted recombinant DNA molecules and a certain amount of genomic DNA are adjacent to both ends of the inserted DNA / flank both ends of the inserted DNA. This trait or transgenic event includes, but is not limited to, resistance to pests and diseases, water use efficiency, yield performance, drought tolerance, seed quality, improved nutritional quality, hybrid seed production and herbicide tolerance, wherein the trait is measured relative to a plant lacking this trait or transgenic event. Specific examples of such advantageous and / or useful properties (traits) are better plant growth, vigor, stress tolerance, stand growth ability, lodging resistance, nutrient uptake, plant nutrition and / or yield, in particular improved growth, increased tolerance to high or low temperatures, increased tolerance to drought or to water or soil salinity levels, enhanced flowering performance, easier harvesting, accelerated maturation, higher yield, higher quality and / or higher nutritional value of the harvested product, better storage life and / or processability of the harvested product, and increased resistance to animal and microbial pests (e.g. insects, arachnids, nematodes, mites, slugs and snails).

[0109] Plants treated with the composition of the present invention include but are not limited to:

[0110] Crops: corn, rice, wheat, barley, rye, oats, sorghum, cotton, soybeans, peanuts, buckwheat, sugar beets, rapeseed, sunflower, sugar cane, tobacco, etc.;

[0111] Vegetables: Solanaceae vegetables (e.g., eggplant, tomato, pimento, pepper, and potato); Cucurbitaceae vegetables (e.g., cucumber, pumpkin, zucchini, watermelon, and cantaloupe); Cruciferous vegetables (e.g., Japanese radish, white radish, horseradish, kohlrabi, Chinese cabbage, cabbage, mustard greens, broccoli, cauliflower); Asteraceae vegetables (e.g., burdock, artemisia, artichoke, and lettuce); Liliaceae vegetables (e.g., shallot, onion, garlic, and asparagus); Ammiaceous vegetables (e.g., carrot, parsley, celery, and parsnip); Chenopodiaceae vegetables (e.g., spinach and Swiss chard); Mint vegetables (e.g., perilla, mint, and basil), strawberry, sweet potato, wild yam, taro, etc.

[0112] Flowers: Decorative foliage plants:

[0113] Fruits: pome fruits (e.g., apples, pears, pears, papayas, and quinces); stone fruits (e.g., peaches, plums, nectarines, black plums, cherries, apricots, and plums); citrus fruits (e.g., mandarin oranges, tangerines, lemons, limes, and grapefruits); nuts (e.g., chestnuts, walnuts, hazelnuts, almonds, pistachios, cashews, and macadamia nuts); berries (e.g., blueberries, cranberries, blackberries, and raspberries); grapes, persimmons, olives, plums, bananas, coffee, dates, coconuts, etc.

[0114] Trees other than fruit trees: tea, mulberry, flowering trees; street trees (e.g., ash, birch, elm, eucalyptus, ginkgo, lilac, maple, oak, poplar, redbud, sweetgum, sycamore, beech, Japanese cedar, fir, hemlock, juniper, pine, spruce and yew); etc.

[0115] The above-mentioned "plants" include genetically modified crops.

[0116] The harmful organisms for which the composition of the present invention has control efficacy include plant pathogens such as filamentous fungi, and specifically include the following examples, but are not limited thereto:

[0117] Rice diseases: Rice blast (Magnaporthe grisea), brown spot (Cochliobolus miyabeanus), sheath blight (Rhizoctonia solani), and seedling blight (Gibberella fujikuroi).

[0118] Wheat diseases: powdery mildew (Erysiphe graminis), head blight (Fusarium gaminearum, F. avenaceum, F. culmorum, Microdochium nivale), rust (Puccinia striiformis, P. graminis, P. recondita), snow mold (Micronectriella nivale), snow rot (Typhula sp.), loose smut (Ustilagotritici), bunt (Tilletia caries), eye stripe (Pseudocercosporella herpotrichoides), leaf blight (Mycosphaerella graminicola), glumen blight (Stagonosporanodorum), and brown spot (Pyrenophora tritici-repentis);

[0119] Barley diseases: powdery mildew (Erysiphe graminis), sickle wilt (Fusarium gaminearum, F. avenaceum, F. culmorum, Microdochium nivale), rust (Puccinia striiformis), wheat stem rust (P. graminis), barley brown rust (P. hordei), loose smut (Ustilago nuda), cloud disease (Rhynchosporium secalis), net spot (Pyrenophorateres), spot disease (Cochliobolus sativus), leaf stripe disease (Pyrenophora graminea), and seedling blight caused by Rhizoctonia solani;

[0120] Corn diseases: Corn leaf spot (Helminthosporium maydis), smut (Ustilago maydis), southern leaf blight (Cochliobolusheterostrophus), leopard disease (Gloeocercosporasorghi), southern rust (Puccinia polysora), gray leaf spot (Cercosporazeae-maydis), and seedling blight caused by Rhizoctonia (Rhizoctonia solani);

[0121] Citrus diseases: black spot (Diaporthecitri), scab (Elsinoefawcetti), fruit rot (Penicillium digitatum), Penicillium italicum, blight (Phytophthora parasitica and Phytophthora citrophthora);

[0122] Apple diseases: apple anthracnose (Colletotrichum gloeosporioides), blossom blight (Moniliniamali), rot (Valsaceratosperma), powdery mildew (Podosphaeraleucotricha), leaf spot (Alternariaalternataapplepathotype), scab (Venturia inaequalis), bitter rot (Colletotrichum acutatum), and crown rot (Phytophthora caudatum).

[0123] Pear diseases: scab (Venturia nashicola, V. pirina), black spot (Alternaria alternate Japanese pear pathotype), rust (Gymnosporangium haraeanum), and phytophthora fruit rot (Phytophtoracactorum);

[0124] Peach diseases: brown rot (Monilinia fructicola), scab (Cladosporium carpophilum), and phomopsis (Phomopsis sp.);

[0125] Grape diseases: Anthracnose (Elsinoeampelina), late rot (Glomerella cingulata), powdery mildew (Uncinulanecator), rust (Phakopsora ampelopsidis), black rot (Guignardia bidwellii) and downy mildew (Plasmoparaviticola).

[0126] Persimmon diseases: Anthracnose (Gloeosporium kaki), leaf spot (Cercospora kaki), Mycosphaerella nawae;

[0127] Cucurbitaceae diseases: anthracnose (Colletotrichum lagenarium), powdery mildew (Sphaerothecafuliginea), vine blight (Mycosphaerellamelonis), fusarium wilt (Fusarium oxysporum), downy mildew (Pseudoperonosporacubensis), blight (Phytophthora sp.), and damping-off (Pythium sp.).

[0128] Tomato diseases: gray mold (Botrytis cinerea), early blight (Alternaria solani), leaf mold (Cladosporium fulvum), and late blight (Phytophthora infestans);

[0129] Eggplant diseases: brown spot (Phomopsis vexans) and powdery mildew (Erysiphe cichoracearum);

[0130] Crucifer diseases: Alternaria leaf spot (Alternaria japonica), white spot (Cercosporella brassicae), clubroot (Plasmodiophorabrassicae), and downy mildew (Peronospora parasitica);

[0131] Onion diseases: rust (Puccinia allii) and downy mildew (Peronosporadestructor);

[0132] Soybean diseases: purple spot (Cercosporakikuchii), scab (Elsinoeglycines), black spot (Diaporthephaseolorum var. sojae), septoria brown spot (Septoriaglycines), gray leaf spot (Cercospora sojina), rust (Phakopsora pachyrhizi), root rot (Phytophthora sojae), aboveground blight caused by Rhizoctonia (Rhizoctonia solani), brown ring rot (Corynespora cassiicola), and sclerotinia (Sclerotinia sclerotiorum).

[0133] Bean diseases: Anthracnose (Colletotrichum lindemthianum);

[0134] Peanut diseases: black spot (Cercospora personata), brown spot (Cercospora arachidicola), and white rot (Sclerotium rolfsii);

[0135] Pea diseases: Powdery mildew (Erysiphe pisi);

[0136] Potato diseases: early blight (Alternaria solani), late blight (Phytophthora infestans), red rot (Phytophthora Erythroseptica), and powdery scab (Spongospora subterraneanf. sp. subterranea);

[0137] Strawberry diseases: powdery mildew (Sphaerothecahumuli) and anthracnose (Glomerella cingulata);

[0138] Tea tree diseases: web cake disease (Exobasidium reticulatum), white spot disease (Elsinoeleucospila), wheel spot disease (Pestalotiopsis sp.), and anthracnose disease (Colletotrichum theae-sinensis);

[0139] Tobacco diseases: brown spot (Alternaria longipes), powdery mildew (Erysiphe cichoracearum), anthracnose (Colletotrichum tabacum), downy mildew (Peronospora tabacina), and blackleg (Phytophthora nicotlanae);

[0140] Oilseed rape diseases: Sclerotinia sclerotiorum and Rhizoctonia solani seedling blight (Rhizoctonia solani);

[0141] Cotton diseases: Seedling blight caused by Rhizoctonia solani (Rhizoctonia solani);

[0142] Sugar beet diseases: Cercospora leaf spot (Cercosporabeticola), leaf blight (Thanatephoruscucumeris), root rot (Thanatephoruscucumeris), and aphanomyces root rot (Aphanomyces cochlioides);

[0143] Rose diseases: black spot (Diplocarponrosae), powdery mildew (Sphaerothecapannosa), and downy mildew (Peronospora sparsa);

[0144] Chrysanthemum diseases: downy mildew (Bremialacctucae), leaf blight (Septoria chrysanthemi-indici), white rust (Puccinia horiana), leaf blight (Septoria chrysanthemi-indici), and white rust (Puccinia horiana);

[0145] Pepper diseases: pepper blight (Phytophthora capsici) and pepper rot (Pythium deliense Meurs);

[0146] Various crop diseases: diseases caused by Pythium spp. (Pythium aphanidermatum, Pythium debarianum, Pythium irregulare and Pythium ultimum), gray mold (Botrytis cinerea) and sclerotinia (Sclerotiniasclerotiorum);

[0147] Radish diseases: Alternaria leaf spot (Alternaria brassicicola);

[0148] Turf diseases: Dollar spot (Sclerotinia homeocarpa), brown spot and giant spot (Rhizoctonia solani);

[0149] Banana diseases: Black leaf spot (Mycosphaerella fijiensis, Mycosphaerella musicola);

[0150] Seed diseases or diseases in the early stages of growth of various plants caused by Aspergillus spp., Penicillium spp., Fusarium spp., Gibberella spp., Tricoderma spp., Thielaviopsis spp., Rhizopus spp., Mucor spp., Corticium spp., Phoma spp., Rhizoctonia spp. or Diplodia spp.; and viral diseases of various plants transmitted by Polymixa or Olpidium, etc.

[0151] The composition has a wide range of efficacy as a crop fungicide. The exact amount of the composition to be applied depends not only on the relative amounts of the components, but also on the specific action desired, the type of phytopathogenic microorganisms to be controlled and their growth stage, and the parts of the plant or other products that will be contacted with the composition. Therefore, formulations containing the composition may not be equally effective or may not be able to resist the same type of phytopathogenic microorganisms at similar concentrations.

[0152] The compositions of the present invention can be applied to phytopathogenic microorganisms or their locus by using conventional ground sprayers, and granular applicators, and by other conventional methods known to those skilled in the art. DETAILED DESCRIPTION

[0153] The present invention is further elaborated below by specific embodiment, in order to have a better understanding of the present invention, but does not constitute limitation of the present invention. The endpoints and any value of the scope disclosed in this article are not limited to the accurate scope or value, and these scopes or values ​​should be understood as containing values ​​close to these scopes or values. For numerical ranges, between the endpoint values ​​of each scope, between the endpoint values ​​of each scope and a separate point value, and between separate point values, one or more new numerical ranges can be combined with each other, and these numerical ranges should be considered as specifically disclosed in this article.

[0154] Several methods for preparing the compounds of formula (I) of the present invention have been disclosed in CN113683509A, CN114890899A, which are incorporated herein by reference. The raw materials can be purchased from the market or can be prepared by methods known in the literature. It will be appreciated by those skilled in the art that other synthetic routes can also be used to synthesize the compounds of the present invention.

[0155] As used herein, oxadiazine (CAS NO: 110488-70-5) is a lipid and membrane synthesis inhibitor, the mechanism of action of which is to destroy the formation of bacterial cell walls, and has the following structure:

[0156]

[0157] As used herein, procymidone (CAS NO: 32809-16-8) is an imide fungicide, which has the following structure:

[0158]

[0159] As used herein, azoxystrobin (CAS NO: 131860-33-8) is a methoxyacrylate fungicide, which has the following structure:

[0160]

[0161] As used herein, difenoconazole (CAS NO: 119446-68-3) is a triazole fungicide, the mechanism of which is to inhibit the biosynthesis of ergosterol in cells, and has the following structure:

[0162]

[0163] As used herein, fluopicolide (CAS NO: 1228284-64-7) is a succinate dehydrogenase inhibitor (SDHI) whose mechanism of action is to interfere with respiratory chain complex II to prevent energy synthesis, and has the following structure:

[0164]

[0165] As used herein, thiabendazole (CAS NO: 148-79-8) is a benzimidazole fungicide, which has the following structure:

[0166]

[0167] As used herein, fluopyram (CAS NO: 130339-07-0) is a pyrimidine amine fungicide, the mechanism of which is to inhibit the activity of 1-NADH oxidoreductase of the FRACC1 subcomplex, and has the following structure:

[0168]

[0169] As used herein, tricyclazole (CAS NO: 41814-78-2) is a thiazole melanin biosynthesis inhibitor-reductase (MBI-R) fungicide, the mechanism of which is mainly to inhibit the formation of appressorial melanin, and has the following structure:

[0170]

[0171] As used herein, pyrochlore (CAS NO: 50512-35-1) is a sulfur-containing organic heterocyclic fungicide, the mechanism of which is to inhibit phospholipid N-methyltransferase, thereby hindering the formation of fatty acids, triglycerides, and phospholipids, and has the following structure:

[0172]

[0173] As used herein, prochloraz (CAS NO: 67747-09-5) is an imidazole fungicide, which has the following structure:

[0174]

[0175] As used herein, rice blast amide (CAS NO: 115852-48-7) is a phenoxyamide fungicide, and its mechanism of action is a melanin biosynthesis inhibitor (MBI-D), which mainly inhibits the activity of spondylospermide dehydrogenase, thereby inhibiting the formation of melanin in rice blast fungus. It has the following structure:

[0176]

[0177] As used herein, pyraclostrobin (CAS NO: 175013-18-0) is a methoxyacrylate fungicide, the mechanism of which is to inhibit mitochondrial respiration, and has the following structure:

[0178]

[0179] As used herein, kasugamycin (CAS NO: 6980-18-3) is an aminoglycoside antibiotic produced by Streptomyces kasuga, and its mechanism of action is to interfere with the esterase system of amino acid metabolism of pathogenic bacteria and destroy protein biosynthesis, and has the following structure:

[0180]

[0181] When it is used as a salt, such as kasugamycin hydrochloride (CAS NO: 19408-46-9), it has the following structure:

[0182]

[0183] As used herein, thiophanate-methyl (CAS NO: 23564-05-8) is a systemic fungicide having the following structure:

[0184]

[0185] As used herein, trifloxystrobin (CAS NO: 141517-21-7) is a strobilurin fungicide having the following structure:

[0186]

[0187] As used herein, Jinggangmycin A (CAS NO: 37248-47-8) is a metabolite produced by actinomycetes, a tyrosinase inhibitor, and has the following structure:

[0188]

[0189] As used herein, tebuconazole (CAS NO: 107534-96-3) is a triazole fungicide, the mechanism of which is to inhibit the biosynthesis of ergosterol in cells, and has the following structure:

[0190]

[0191] As used herein, Isopropylamine (CAS NO: 26087-47-8) is an organophosphorus fungicide, the mechanism of which is to interfere with cell membrane permeability, prevent certain lipophilic chitin precursors from passing through the cytoplasmic membrane, hinder the synthesis of chitin, prevent the cell wall from growing, and inhibit the normal development of bacteria. It has the following structure:

[0192]

[0193] As used herein, fluazinam (CAS NO: 79622-59-6) is a dinitroaniline fungicide, whose mechanism of action is to uncouple oxidation and phosphorylation at the end of the respiratory chain, consume the electrochemical potential energy accumulated by electron transfer to the maximum extent, block the formation of pathogen energy (ATP), and thus kill the pathogen. It has the following structure:

[0194]

[0195] All plant pathogenic microorganisms in the biological examples were from strains stored at 4°C in the laboratory of the College of Plant Protection of Nanjing Agricultural University.

[0196] When analyzing the interaction of compound fungicides, the effect of the compound is often judged by the synergistic coefficient SR value. The synergistic effect of the compound fungicide is judged according to the synergistic coefficient (SR) value. That is, SR>1.5 is a synergistic effect, 1.5≥SR≥0.5 is an additive effect, and SR<0.5 is an antagonistic effect. The synergistic coefficient is calculated according to the following formula (1) and formula (2).

[0197] L=(PA×A+PB×B) / (PA+PB)×100% (1)

[0198] Where:

[0199] L---EC of compound 50 Theoretical value, in micrograms per milliliter (μg / mL)

[0200] PA--the value of A in the ratio of compound A to B

[0201] PB--the value of B in the ratio of compound A to B

[0202] A---EC of A in the compound 50 Value, in micrograms per milliliter (μg / mL)

[0203] B---EC of B in the compound 50 Value, in micrograms per milliliter (μg / mL)

[0204] SR=L / S (2)

[0205] Where:

[0206] SR--synergistic coefficient of compound agent

[0207] L---EC of compound 50 Theoretical value, in micrograms per milliliter (μg / mL)

[0208] S---EC of compound 50 Actual value, in micrograms per milliliter (μg / mL)

[0209] Collaborative computing:

[0210] A synergistic effect exists whenever the effect of a combination of active ingredients is greater than the sum of the effects of the individual components.

[0211]

[0212] Wherein E0 represents the expected percentage of protection of the combination of two active components at a specified dose (e.g., equal to x and y, respectively), X is the percentage of protection observed by component (A) at a specified dose (equal to x), and Y is the percentage of protection observed by component (B) at a specified dose (equal to y). When the percentage of protection observed for the combination (E) is greater than the expected percentage (E0), that is, E-E0>0, there is a synergistic effect.

[0213] Antibacterial concentration EC 50 The values, toxicity regression equations and correlation coefficients were calculated by the DPS data processing system: the actual test concentration and the corresponding spore germination inhibition rate were input, quantitative biological machine value analysis was used, the measurements were converted into logarithmic units, and the lethal mortality value was set to 50 to obtain the required data.

[0214] definition:

[0215] Water agar medium: add 20 g agar to 1000 mL of distilled water, stir to fully dissolve, divide into 200 mL Erlenmeyer flasks, sterilize at 121°C for 20 minutes, and set aside after cooling.

[0216] PDA medium (potato agar glucose medium): wash and peel 200g of potatoes, cut into small pieces, boil them in boiling water (boil for 30 minutes), filter with four layers of gauze, remove the residue, add 20g of glucose, then add 20g of agar, add double distilled water to make up to 1000mL, stir to fully dissolve, divide into 200mL Erlenmeyer flasks, sterilize at 121℃ for 20 minutes, cool and set aside.

[0217] V8 medium (10%): add 1g CaCO3 to 100mL V8 juice, centrifuge at 2500rpm for 5min, dilute the supernatant with double distilled water at a ratio of 1:9. If preparing solid medium, add 15g agar powder to 1L. Dispense into 200mL Erlenmeyer flasks, sterilize at 121℃ for 20min, and cool for later use.

[0218] PDAG culture medium: Wash and peel 200g of potatoes, cut into small pieces, boil them in boiling water (boil for 30 minutes), filter with four layers of gauze, remove the residue, add 20g of mannitol, then add 20g of agar, make up to 1000mL with double distilled water, stir to fully dissolve, divide into 200mL Erlenmeyer flasks, sterilize at 121℃ for 20 minutes, and set aside after cooling.

[0219] Preparation method of CM medium: Measure 50 mL of 20× nitrate (120 g sodium nitrate, 10.4 g potassium chloride, 10.4 g magnesium sulfate heptahydrate, 30.4 g potassium dihydrogen phosphate, add distilled water to dissolve to 1 L), 1000× trace elements (2.2 g zinc sulfate heptahydrate, 1.1 g boric acid, 0.5 g manganese chloride tetrahydrate, 0.5 g iron sulfate heptahydrate, 0.17 g cobalt chloride hexahydrate, 0.16 g copper sulfate pentahydrate, 0.15 g sodium manganate dihydrate, 5 g Tetrasodium EDTA, add distilled water to dissolve to 100mL) 1mL, vitamin solution (0.01g biotin, 0.01g vitamin B6, 0.01g vitamin B1, 0.01g riboflavin, 0.01g p-aminobenzoic acid, 0.01g niacin, add distilled water to dissolve to 100mL) 1mL, weigh 10g glucose, 2g peptone, 1g yeast extract, 1g casamino acid, 15g agar powder, add distilled water to quantify to 1L, divide into Erlenmeyer flasks, sterilize at 121℃ for 20 minutes, cool and set aside.

[0220] Preparation method of spore-forming culture medium (SDC): prepare it with corn flour and rice straw, weigh 100g rice straw, add 1L water and boil for 30 minutes, then add 40g corn flour and 15g agar powder and boil for 20 minutes, finally make up to 1L with distilled water, divide it into Erlenmeyer bottles, sterilize at 121℃ for 20 minutes, and set aside after cooling.

[0221] Biological Example 1: Pyricularia grisea (Rice Blast)

[0222] The test compound (content> 98%) was dissolved in DMSO to prepare a mother solution with a concentration of 10000 ppm. The mother solution of compound (I) and the mother solutions of different compounded agents were compounded in different proportions, and the agent concentration was diluted to the concentration shown in Table 1. The spore suspension of rice blast fungus (1×10 5 / mL), and add Tween 80 in equal proportion. 50μL of spore suspension was dropped on each plate, and evenly spread with a sterilized glass rod. Different treatments were made according to different spore germination conditions (generally germination takes about 4 hours), and 200 spores were counted under a microscope (the field of view was not repeated), and the number of germination was recorded. The results are shown in Tables 1 and 2.

[0223] Table 1 Effects of compound (I) and component (B) used alone or in combination on rice blast fungus

[0224]

[0225]

[0226]

[0227] Table 2 Synergistic effect of compound (I) and component (B) used alone or in combination on rice blast fungus

[0228] Composition (A+B) Ratio (A:B) Regression equation Correlation coefficient <![CDATA[EC 50 Value]]> SR value Compound (I) - y=2.7766x+3.7764 0.9478 2.7586 - Compound (I) + enoylmorpholine 3:1 y=4.0927+2.6879x 0.9626 2.1755 1.0865 Compound (I) + enoylmorpholine 1:1 y=4.2579+2.8330x 0.9825 1.8278 1.0770 Compound (I) + enoylmorpholine 1:3 y=4.1812+1.5300x 0.9999 3.4287 0.4589 Dimethomorph - y=3.3720+1.7503x 0.9333 8.5793 Compound (I) + procymidone 3:1 y=1.3126+4.2894x 0.9805 7.2387 1.2576 Compound (I) + procymidone 1:1 y=4.0219+1.4190x 0.7633 4.8901 3.1590 Compound (I) + procymidone 1:3 y=4.2165+1.8558x 0.9857 2.6437 8.2432 Procymidone - y=2.2530+1.8954x 0.9344 28.1373 - Compound (I) + azoxystrobin 3:1 y=7.1816+2.1107x 0.9417 0.0926 23.2198 Compound (I) + azoxystrobin 1:1 y=6.3314+1.2235x 0.9641 0.0816 18.8934 Compound (I) + azoxystrobin 1:3 y=6.1473+2.1118x 0.9713 0.2862 3.2608 Azoxystrobin - y=6.1699+2.3951x 0.9667 0.3248 - Compound (I) + difenoconazole 3:1 y=4.0214+1.8725x 0.9128 3.3255 1.2233 Compound (I) + difenoconazole 1:1 y=3.5894+3.4888x 0.9518 2.5371 2.1195 Compound (I) + difenoconazole 1:3 y=3.8969+2.1898x 0.9596 3.1898 2.0963 Difenoconazole - y=0.2216+5.7832x 0.9726 7.9962 - Compound (I) + fluopicolide 3:1 y=5.4953+1.6431x 0.9702 0.4995 4.8006 Compound (I) + fluopicolide 1:1 y=5.0819+1.3655x 0.9585 0.8710 2.3389 Compound (I) + fluopicolide 1:3 y=4.8546+1.4856x 0.9641 1.2528 1.3382 Fluoxathiapiprolin - y=4.8075+1.6146x 0.9684 1.3158 - Compound (I) + Thiabendazole 3:1 y=4.2297+1.4266x 0.9869 3.4668 1.6307 Compound (I) + Thiabendazole 1:1 y=4.0496+1.4217x 0.9981 4.6614 1.8337 Compound (I) + Thiabendazole 1:3 y=3.7130+1.3863x 0.9963 8.4787 1.3495 Thiabendazole - y=3.4091+1.3757x 0.9996 14.3368 - Compound (I) + fluopyram 3:1 y=5.1397+1.5094x 0.9935 0.8081 3.0365 Compound (I) + fluopyram 1:1 y=5.2425+1.5661x 0.9931 0.7001 3.0696 Compound (I) + fluopyram 1:3 y=5.0099+1.6243x 0.9995 0.9860 1.8705 Fluoxazolidinone - y=4.7234+1.4763x 0.9818 1.5395 -

[0229] Biological Example 2: Fusarium graminearum (wheat head blight)

[0230] The test compound (content> 98%) was dissolved in DMSO to prepare a mother solution with a concentration of 10000 ppm. The mother solution of compound (I) and the mother solutions of different compounded agents were compounded in different proportions, and the agent concentration was diluted to a suitable gradient concentration (as shown in Table 3 below). The spore suspension of Fusarium graminearum (1×10 5 / mL), and add Tween 80 in equal proportion. 50μL of spore suspension was dropped on each plate, and evenly coated with a sterilized glass rod. Different treatments were made according to different spore germination conditions (generally germination takes about 4 hours), and 200 spores were counted under a microscope (the field of view was not repeated), and the number of germination was recorded. The results are shown in Tables 3 and 4.

[0231] Table 3 Effects of compound (I) and component (B) used alone or in combination against Fusarium graminearum

[0232]

[0233]

[0234] Table 4 Synergistic effect of compound (I) and component (B) used alone or in combination on Fusarium graminearum

[0235] Composition (A+B) Ratio (A:B) Regression equation Correlation coefficient <![CDATA[EC 50 Value]]> SR value Compound (I) - y=3.6723+1.1824x 0.9997 13.273 - Compound (I) + enoylmorpholine 3:1 y=3.9961+1.2155x 0.9980 6.6980 2.3382 Compound (I) + enoylmorpholine 1:1 y=3.7542+1.2210x 0.9992 10.4789 1.7224 Compound (I) + enoylmorpholine 1:3 y=3.6796+1.0804x 0.9986 16.6783 1.2254 Dimethomorph - y=3.5314+1.0811x 0.9969 22.8249 - Compound (I) + azoxystrobin 3:1 y=9.1913+4.3772x 0.8933 0.1103 90.9594 Compound (I) + azoxystrobin 1:1 y=9.4930+5.2670x 0.9408 0.1403 48.4152 Compound (I) + azoxystrobin 1:3 y=6.9209+1.4725x 0.9558 0.0496 71.6225 Azoxystrobin - y=5.5024+0.9939x 0.9862 0.3123 - Compound (I) + difenoconazole 3:1 y=4.3819+1.2436x 0.9681 3.1408 3.5866 Compound (I) + difenoconazole 1:1 y=4.3932+1.2617x 0.9685 3.0266 3.0585 Compound (I) + difenoconazole 1:3 y=4.4580+1.2872x 0.9794 2.637 2.7488 Difenoconazole - y=4.0856+1.2711x 0.9952 5.2405 - Compound (I) + fluopicolide 3:1 y=6.8753+1.3795x 0.9780 0.0437 228.3959 Compound (I) + fluopicolide 1:1 y=7.3981+1.3444x 0.9905 0.0165 405.3818 Compound (I) + fluopicolide 1:3 y=7.1141+1.2584x 0.9869 0.0209 162.5215 Fluoxathiapiprolin - y=6.5902+1.6222x 0.9515 0.1046 - Compound (I) + Thiabendazole 3:1 y=4.3770+1.0729x 0.9999 3.8080 3.0162 Compound (I) + Thiabendazole 1:1 y=4.4920+1.1124x 0.9927 2.8619 3.3889 Compound (I) + Thiabendazole 1:3 y=4.7330+1.3390x 0.9958 1.5827 4.9988 Thiabendazole - y=4.1565+1.0717x 0.9924 6.1245 - Compound (I) + fluopyram 3:1 y=5.0087+1.3182x 0.9985 0.9849 10.5060 Compound (I) + fluopyram 1:1 y=4.9081+1.2085x 0.9996 1.1914 6.2294 Compound (I) + fluopyram 1:3 y=5.0890+1.2454x 0.9928 0.8482 5.3008 Fluoxazolidinone - y=4.7491+1.2796x 0.9944 1.5705 -

[0236] Biological Example 3: Botrytis cinerea (Tomato Gray Mold)

[0237] The test compound (content> 98%) was dissolved in DMSO to prepare a mother solution with a concentration of 10000 ppm. The mother solution of compound (I) and the mother solutions of different compounded agents were compounded in different proportions, and the agent concentration was diluted to a suitable gradient concentration (as shown in Table 5 below). The spore suspension of Botrytis cinerea (1×10 5 / mL), and add equal proportion of Tween 80. 50 μL of spore suspension was dropped on each plate, and evenly coated with a sterilized glass rod. Different treatments were made according to different spore germination conditions (generally germination takes about 12 hours), and 200 spores were counted under a microscope (the field of view was not repeated), and the number of germination was recorded. The results are shown in Tables 5 and 6.

[0238] Table 5 Effect of compound (I) and component (B) used alone or in combination on Botrytis cinerea

[0239]

[0240]

[0241]

[0242] Table 6 Synergistic effect of compound (I) and component (B) used alone or in combination on Botrytis cinerea

[0243]

[0244]

[0245] Biological Example 4: Phytophthora sojae (soybean phytophthora)

[0246] The test compound (content> 98%) was dissolved in DMSO to prepare a mother solution with a concentration of 10000 ppm. The mother solution of compound (I) and the mother solutions of different compounded agents were compounded in different proportions, and the agent concentration was diluted to a suitable gradient concentration (as shown in Table 7 below). The soybean spore suspension (1×10 5 / mL), and add Tween 80 in equal proportion. 50 μL of spore suspension was dropped on each plate, and evenly coated with a sterilized glass rod. Different treatments were made according to different spore germination conditions (generally germination takes about 20 hours), and 200 spores were counted under a microscope (the field of view was not repeated), and the number of germination was recorded. The results are shown in Tables 7 and 8.

[0247] Table 7 Effects of compound (I) and component (B) used alone or in combination against Phytophthora sojae

[0248]

[0249]

[0250]

[0251] Table 8 Synergistic effect of compound (I) and component (B) used alone or in combination on Phytophthora sojae

[0252]

[0253]

[0254] Biological Example 5: Phytophthora capsici (Pepper Phytophthora)

[0255] The test compound (content> 98%) was dissolved in DMSO to prepare a mother solution with a concentration of 10000 ppm. The mother solution of compound (I) and the mother solutions of different compounded agents were compounded in different proportions, and the agent concentration was diluted to a suitable gradient concentration (as shown in Table 9 below). The spore suspension of Phytophthora capsici (1×10 5 / mL), and add Tween 80 in equal proportion. 50 μL of spore suspension was dripped onto each plate, and evenly coated with a sterilized glass rod. Different treatments were made according to different spore germination conditions (generally germination takes about 20 hours), and 200 spores were counted under a microscope (the field of view was not repeated), and the number of germinations was recorded. The results are shown in Tables 9 and 10.

[0256] Table 9 Effect of compound (I) and component (B) used alone or in combination on Phytophthora capsici

[0257]

[0258]

[0259]

[0260] Table 10 Synergistic effect of compound (I) and component (B) used alone or in combination on Phytophthora capsici

[0261]

[0262] Biological Example 6: Phytophthora infestans (potato late blight)

[0263] The test compound (content> 98%) was dissolved in DMSO to prepare a mother solution with a concentration of 10000 ppm. The mother solution of compound (I) and the mother solutions of different compounded agents were compounded in different proportions, and the agent concentration was diluted to a suitable gradient concentration (as shown in Table 11 below). The spore suspension of Phytophthora infestans (1×10 5 / mL), and add Tween 80 in equal proportion. 50 μL of spore suspension was dropped on each plate, and evenly coated with a sterilized glass rod. Different treatments were made according to different spore germination conditions (generally germination takes about 24 hours), and 200 spores were counted under a microscope (the field of view was not repeated), and the number of germination was recorded. The results are shown in Tables 11 and 12.

[0264] Table 11 Effects of compound (I) and component (B) used alone or in combination against Phytophthora infestans

[0265]

[0266]

[0267]

[0268] Table 12 Synergistic effect of compound (I) and component (B) used alone or in combination on Phytophthora infestans

[0269]

[0270]

[0271] Biological Example 7: Helminthosporium (corn leaf spot)

[0272] The test compound (content> 98%) was dissolved in DMSO to prepare a mother solution with a concentration of 10000 ppm. The mother solution of compound (I) and the mother solutions of different compounded agents were compounded in different proportions, and the agent concentration was diluted to a suitable gradient concentration (as shown in Table 13 below). The spore suspension of Helminthosporium (1×10 5 / mL), and add Tween 80 in equal proportion. 50 μL of spore suspension was dripped onto each plate, and evenly spread with a sterilized glass rod. Different treatments were made according to different spore germination conditions (generally germination takes about 8 hours), and 200 spores were counted under a microscope (the field of view was not repeated), and the number of germinations was recorded. The results are shown in Tables 13 and 14.

[0273] Table 13 Effect of compound (I) and component (B) used alone or in combination on Helminthosporium

[0274]

[0275]

[0276]

[0277] Table 14 Synergistic effect of compound (I) and component (B) used alone or in combination on Helminthosporium

[0278] Composition (A+B) Ratio (A:B) Regression equation Correlation coefficient <![CDATA[EC 50 Value]]> SR value Compound (I) - y=4.6499+0.5106x 0.9989 4.850 - Compound (I) + enoylmorpholine 3:1 y=3.5536+1.7795x 0.9976 6.4981 1.5615 Compound (I) + enoylmorpholine 1:1 y=3.4623+1.7462x 0.9990 7.5965 2.0331 Compound (I) + enoylmorpholine 1:3 y=3.4171+1.7244x 0.9994 8.2786 2.5054 Dimethomorph - y=3.3363+1.1753x 0.9966 26.0383 - Compound (I) + azoxystrobin 3:1 y=5.4436+1.4195x 0.9987 0.4870 8.1582 Compound (I) + azoxystrobin 1:1 y=5.1701+1.3993x 0.9995 0.7545 4.1035 Compound (I) + azoxystrobin 1:3 y=4.9095+1.4749x 0.9994 1.1517 1.9268 Azoxystrobin - y=4.8183+1.4215x 0.9998 1.3422 - Compound (I) + difenoconazole 3:1 y=3.9912+1.2747x 0.9905 6.1862 1.0104 Compound (I) + difenoconazole 1:1 y=3.9797+1.1411x 0.9940 7.8372 0.9763 Compound (I) + difenoconazole 1:3 y=3.9252+1.0891x 0.9991 9.7029 0.9329 Difenoconazole - y=3.8865+1.0925x 0.9995 10.4527 - Compound (I) + fluopicolide 3:1 y=4.4315+1.8129x 0.9998 2.0587 3.0078 Compound (I) + fluopicolide 1:1 y=4.0287+1.4801x 0.9994 4.5317 1.6625 Compound (I) + fluopicolide 1:3 y=3.8564+1.1923x 0.9918 9.1039 0.9750 Fluoxathiapiprolin - y=3.7049+1.2831x 0.9963 10.2183 - Compound (I) + Thiabendazole 3:1 y=4.4124+1.8386x 0.9994 2.0874 2.7154 Compound (I) + Thiabendazole 1:1 y=4.0220+1.3940x 0.9995 5.0305 1.2894 Compound (I) + Thiabendazole 1:3 y=3.7722+1.4570x 0.9977 6.9618 1.0492 Thiabendazole - y=3.6081+1.5301x 0.9973 8.1226 - Compound (I) + fluopyram 3:1 y=4.3623+1.7133x 0.9986 2.3563 1.9794 Compound (I) + fluopyram 1:1 y=4.1432+2.0149x 0.9995 2.6626 1.6818 Compound (I) + fluopyram 1:3 y=4.0255+1.7383x 0.9992 3.6358 1.1805 Fluoxazolidinone - y=3.9476+1.7156x 0.9992 4.1061 -

[0279] Biological Example 8: Colletotrichum gloeosporioides (Apple Anthracnose)

[0280] The test compound (content> 98%) was dissolved in DMSO to prepare a mother solution with a concentration of 10000 ppm. The mother solution of compound (I) and the mother solutions of different compounded agents were compounded in different proportions, and the agent concentration was diluted to a suitable gradient concentration (as shown in Table 15 below). The spore suspension of Colletotrichum gloeosporioides (1×10 5 / mL), and add Tween 80 in equal proportion. 50 μL of spore suspension was dripped onto each plate, and evenly spread with a sterilized glass rod. Different treatments were made according to different spore germination conditions (generally germination takes about 6 hours), and 200 spores were counted under a microscope (the field of view was not repeated), and the number of germinations was recorded. The results are shown in Tables 15 and 16.

[0281] Table 15 Effect of compound (I) and component (B) used alone or in combination on Colletotrichum gloeosporioides

[0282]

[0283]

[0284]

[0285] Table 16 Synergistic effect of compound (I) and component (B) used alone or in combination on Colletotrichum gloeosporioides

[0286]

[0287]

[0288] Biological Example 9: Pythium delhiii (Pepper rot)

[0289] The test compound (content> 98%) was dissolved in DMSO to prepare a mother solution with a concentration of 10000 ppm. The mother solution of compound (I) and the mother solutions of different compounded agents were compounded in different proportions, and the agent concentration was diluted to a suitable gradient concentration (as shown in Table 17 below). The spore suspension of Pythium delhiense (1×10 5 / mL), and add Tween 80 in equal proportion. 50 μL of spore suspension was dripped onto each plate, and evenly coated with a sterilized glass rod. Different treatments were made according to different spore germination conditions (generally germination takes about 12 hours), and 200 spores were counted under a microscope (the field of view was not repeated), and the number of germinations was recorded. The results are shown in Tables 17 and 18.

[0290] Table 17 Effect of compound (I) and component (B) used alone or in combination against Pythium delhiense

[0291]

[0292]

[0293]

[0294] Table 18 Synergistic effect of compound (I) and component (B) used alone or in combination on Pythium delhiensis

[0295]

[0296]

[0297] Biological Example 10: Synergistic test of the combination of compound (I) and component (B) against rice blast

[0298] This example uses the wild-type strain Guy11 of rice blast fungus. The rice blast fungus strain is placed on complete medium (CM) and cultured in the dark at 28°C. The method of spraying rice leaves with spores of rice blast fungus is as follows:

[0299] 1. Induce rice blast fungus to produce conidia: inoculate the mycelium block (2 mm × 2 mm) of the rice blast fungus strain Guy11 on CM medium onto SDC medium, culture in the dark at 28°C for 4 days, then scrape off the surface mycelium, induce under black light for 3 days to obtain conidia.

[0300] 2. Collect 4 mL of conidia from the above SDC plate at a concentration of 1×10 5 / mL, and contains gelatin with a total concentration of 0.2% (w / v) for use.

[0301] 3. Rice seedlings cultured in the greenhouse for 14 days were used for spray inoculation experiments. A mixture of compound (I) and fungicide (B) of different concentrations was applied one day in advance. The mixture was sprayed on rice leaves at the 3-leaf stage for 24 hours, and then the above-mentioned rice blast fungus spore suspension was sprayed; the dark box temperature was 28°C and the humidity was 90%, and the dark moisturizing culture was carried out for 24 hours, and then 16 hours of light / 8 hours of darkness were alternately used for moisturizing culture for 5 days, and each treatment was repeated three times.

[0302] The results are shown in Tables 19 and 20. Control effect (%) = (disease area of ​​control rice - disease area of ​​treated rice) / (disease area of ​​control rice) × 100.

[0303] Table 19 Application amount of component (A) and component (B) used alone or in combination

[0304]

[0305]

[0306]

[0307] Table 20 Effects of compound (I) and component (B) used alone or in combination on rice blast

[0308]

[0309]

[0310] Through the above experiments, it can be seen that the combination of the compound of formula (I) and various components (B) exhibits a strong synergistic effect against various pathogens.

Claims

1. A composition comprising a mixture of components (A) and (B), wherein: Component (A) is a compound having formula (I): Component (B) is a compound selected from the group consisting of: A) Respiratory depressants - Complex III inhibitors at the Qo site: azoxystrobin, coumoxystrobin, dimoxystrobin, fenaminstrobin, flufenoxystrobin, fluoxastrobin, kresoxim-methyl, picoxystrobin, pyraclostrobin, pyrametostrobin, pyraoxystrobin, trifloxystrobin, triclopyricarb, famoxadone, metyltetraprole; -Qi site complex III inhibitors: cyazofamid, amisulbrom, fenpicoxamid, florylpicoxamid, metarylpicoxamid; - Complex II inhibitors: benzovindiflupyr, bixafen, boscalid, fluopyram, fluxapyroxad, furametpyr, isofetamid, isopyrazam, mepronil, penflufen, sedaxane, thifluzamide, flubeneteram, pydiflumetofen, pyraziflumid, fluindapyr, penthiopyrad, inpyrfluxam, isofluxuram, pyrapropoyne, cyclobutrifluram; - Other respiratory inhibitors: fluazinam, ametoctradin and silthiofam; Complex II inhibitors: diflumetorim; B) Sterol biosynthesis inhibitors -C14 demethylase inhibitors: Triazoles: azaconazole, bitertanol, bromuconazole, cyproconazole, difenoconazole, diniconazole, diniconazole-M, epoxiconazole, fenbuconazole, fluquinconazole, flusilazole, flutriafol, hexaconazole, imibenconazole, ipconazo le), metconazole, myclobutanil, oxpoconazole, penconazole, propiconazole, prothioconazole, simeconazole, tebuconazole, tetraconazole, triadimefon, triadimenol, triticonazole, uniconazole, fluoxytioconazole, ipfentrifluconazole, mefentrifluconazole; Imidazoles: imazalil, pefurazoate, prochloraz, triflumizole; pyrimidines, pyridines and piperazines: fenarimol, nuarimol, pyrifenox, triforine; -δ14-reductase inhibitors: aldimorph, dodemorph, dodemorph-acetate, fenpropimorph, tridemorph, fenpropidin, piperalin, spiroxamine; -3-Ketoreductase inhibitors: fenhexamid; C) Nucleic acid synthesis inhibitors - Phenylamide or acylamino acid fungicides: benalaxyl, benalaxyl-M, metalaxyl, mefenoxam, ofurace, oxadixyl; -Others: octhilinone, bupirimate; D) Inhibitors of cell division and cytoskeleton - Tubulin inhibitors: benomyl, carbendazim, fuberidazole, thiabendazole, thiophanate-methyl; - Other cell division inhibitors: diethofencarb, ethaboxam, pencycuron, fluopicolide, zoxamide, metrafenone, pyriofenone; E) Amino Acids and Protein Synthesis Inhibitors -Methionine synthesis inhibitors (anilinopyrimidines): cyprodinil, mepanipyrim, pyrimethanil; - Protein synthesis inhibitors: blasticidin-S, kasugamycin, kasugamycin hydrochloride-hydrate, streptomycin, oxytetracycline, validamycin A; F) Signal transduction inhibitors - MAP / histidine protein kinase inhibitors: fluoroimide, iprodione, procymidone, vinclozolin, fenpiclonil, fludioxonil; - G protein inhibitor: quinoxyfen; G) Lipid and membrane synthesis inhibitors - Phospholipid biosynthesis inhibitors: edifenphos, iprobenfos, pyrazophos, isoprothiolane; - Lipid peroxidation: dicloran, quintozene, tecnazene, tolclofos-methyl, chloroneb, etridiazole; - Phospholipid biosynthesis and cell wall deposition: dimethomorph, flumorph, mandipropamid, benthiavalicarb, iprovalicarb, valifenalate; - Compounds and fatty acids that affect cell membrane permeability: propamocarb, propamocarb-hydrochlorid; - Oxysterol-binding protein (OSBP) inhibitors: oxathiapiprolin, fluoxapiprolin; H) Inhibitors with multi-site action - Inorganic active substances: Bordeaux mixture, copper acetate, copper hydroxide, copper oxychloride, basic copper sulfate, sulfur; -Thio- and dithiocarbamates: ferbam, mancozeb, maneb, metam, metiram, propineb, thiram, zineb, ziram; - Organochlorine compounds: anilazine, chlorothalonil, captafol, captan, folpet, dichlofluanid, dichlorophen, flusulfamide, hexachlorobenzene, phthalide, tolylfluanid; - Guanidines and others: dodine, guazatine, guazatine acetates, iminoctadine, iminoctadine-triacetate, iminoctadine-tris(albesilate), dithianon; I) Cell wall synthesis inhibitors - Glucan synthesis inhibitors: validamycin, polyoxin D; melanin synthesis inhibitors: pyroquilon, tricyclazole, carpropamid, fenoxanil.

2. The composition according to claim 1, characterized in that When the composition is a two-component composition, the weight ratio of component (A) to component (B) is 400:1 to 1:50, preferably, the weight ratio of component (A) to component (B) is from 200:1 to 1:10, and more preferably, the weight ratio of component (A) to component (B) is from 20:1 to 1:

5.

3. The composition according to claim 1, characterized in that The complex III inhibitor at the Qo site is selected from kresoxim-methyl, azoxystrobin, pyraclostrobin or trifloxystrobin; The complex II inhibitor is selected from pydiflumetofen, boscalid or fluopyram; The complex I inhibitor is selected from diflumetorim; Other respiratory depressants are selected from fluazinam; The C14 demethylase inhibitor is selected from difenoconazole, tebuconazole, metconazole, prothioconazole or prochloraz; The nucleic acid synthesis inhibitor is selected from metalaxyl or metalaxyl-M (mefenoxam); The microtubule inhibitor is selected from carbendazim, thiophanate-methyl or thiabendazole; The protein synthesis inhibitor is selected from kasugamycin, kasugamycin hydrochloride-hydrate or validamycin A; The signal transduction inhibitor is selected from procymidone or fludioxonil; The lipid and membrane synthesis inhibitor is selected from dimethomorph, isoprothiolane or iprobenfos; The inhibitor with multi-site action is selected from Bordeaux mixture, sulfur, mancozeb or chlorothalonil; The melanin synthesis inhibitor is selected from tricyclazole or fenoxanil; More preferably, the complex III inhibitor at the Qo site is selected from azoxystrobin, pyraclostrobin or trifloxystrobin; The complex II inhibitor is selected from pydiflumetofen; The complex I inhibitor is selected from diflumetorim; Other respiratory depressants are selected from fluazinam; The C14 demethylase inhibitor is selected from difenoconazole, tebuconazole or prochloraz; The protein synthesis inhibitor is selected from kasugamycin, kasugamycin hydrochloride-hydrate or validamycin A; The microtubule inhibitor is selected from thiophanate-methyl or thiabendazole; The signal transduction inhibitor is selected from procymidone; The lipid and membrane synthesis inhibitor is selected from dimethomorph, isoprothiolane or iprobenfos; The melanin synthesis inhibitor is selected from tricyclazole or fenoxanil.

4. The composition according to any one of claims 1 to 3, characterized in that The invention also comprises at least one auxiliary agent selected from solvents, fillers or carriers, emulsifiers, dispersants, wetting agents, spreaders, adhesives, stabilizers or synergists.

5. The composition according to any one of claims 1 to 3, characterized in that The composition includes component (A) and component (B) in an amount ranging from about 1 wt % to 90 wt % based on the total weight of the composition.

6. A method for treating plants to resist infection by plant pathogenic microorganisms, the method comprising applying to the plants a composition of an effective amount of component (A) and an effective amount of at least one component (B), thereby treating the plants to resist infection by plant pathogenic microorganisms, the composition being a composition as claimed in claims 1 to 5.

7. A method for controlling plant diseases caused by plant pathogenic microorganisms, the method comprising applying an effective amount of a composition of component (A) and an effective amount of at least one component (B) to the plant pathogenic microorganisms and their habitats or their locations, the composition being the composition of claims 1 to 5.

8. A method for protecting plant propagation material, the method comprising treating the plant propagation material with a composition comprising an effective amount of component (A) and an effective amount of at least one component (B), the composition being a composition as claimed in claims 1 to 6.

9. The method according to claim 8, characterized in that: The plants or their propagation materials are transgenic plants or their seeds, or plants or their seeds processed by genome editing technology.

10. Use of the composition according to any one of claims 1 to 5 for controlling or inhibiting plant pathogenic microorganisms, preventing infection by plant pathogenic microorganisms or protecting plant materials from infection and destruction by pathogenic microorganisms.

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