Insecticidal composition as well as preparation method and application thereof

Abstract

The invention discloses an insecticidal composition as well as a preparation method and application thereof, and relates to but not limited to the technical field of pesticides. The effective components of the insecticidal composition comprise the fluxazole amide and the spirodiclofen diester, different types of components are compounded, and the insecticidal composition has an obvious synergistic effect on cotton bollworms, Turkey spider mites, tetranychus urticae, tetranychus truncatus, ostrinia nubilalis, thrips and the like through multiple different action modes; the composition provided by the invention has the advantages that the effect of the composition is obviously superior to that of preparations such as a fluxazole amide single dosage and a spirodiclofen diester single dosage, the target range of the two insecticides can be expanded while the dosage of the fluxazole amide and the spirodiclofen diester pesticides is effectively reduced, the pesticide application frequency is reduced, and the production cost is reduced.

Description

Technical Field

[0001] The present invention relates to the field of pesticide technology but is not limited thereto, and relates to an insecticide composition and a preparation method and application thereof. Background Art

[0002] According to surveys, there are hundreds of pests that harm crops such as cotton, corn, and eggplant in my country. The annual yield loss of cotton, corn, and eggplant caused by pests is about 20%, and in severe cases, it can reach more than 50%. For example, cotton bollworm, Turkestan spider mite, two-spotted spider mite, truncate spider mite, corn borer, thrips, and aphids, etc. In the process of pest control, the long-term excessive use of chemical pesticides has increased pest resistance and reduced control effects, causing serious agricultural non-point source pollution and affecting ecological and environmental safety. The rational use of mixed agents is one of the important ways to delay resistance and improve drug efficacy. Therefore, it is of great economic value to screen out new high-efficiency, low-toxic, and low-residue environmentally friendly insecticide formulas.

[0003] Fluoxathiapiprolin is an isoxazole insecticide developed by Nissan Chemical Co., Ltd. It has a novel mechanism of action and is an allosteric modulator of γ-aminobutyric acid (GABA)-gated chloride channels. IRAC classifies it as Group 30. It has high insecticidal activity against many types of pests such as Lepidoptera, Hemiptera, Thysanoptera, Coleoptera, Diptera and Acarina, and has high ecological safety for mammals, crops and the environment, and has little impact on pollinating insects.

[0004] Spirobudic acid diepterin is a quaternary ketoacid ester acaricide. It is not systemic and mainly controls eggs, nymphs and female adult mites through contact and stomach poisoning. Its mechanism of action is to inhibit fat synthesis in the body of harmful mites and block energy metabolism. It has no cross-resistance with other acaricides. It has an outstanding egg-killing effect and has good control effects on harmful mites at different developmental stages. It can be used to control mites in crops such as citrus, grapes, eggplants, and peppers.

[0005] There is no report on the insecticide composition consisting of fluoxetine and spirobudicola. Summary of the invention

[0006] One of the purposes of the present invention is to provide an insecticidal composition, which can effectively prevent and control pests, reduce the number of pests, reduce the degree of harm caused by pests to crops such as cotton, corn, eggplant, etc., and increase the yield of cotton, corn, eggplant, etc.

[0007] The second object of the present invention is to provide a method for preparing the above insecticide composition, which is simple and suitable for industrial production.

[0008] The third object of the present invention is to provide a novel, highly effective, low-toxic and low-residue insecticide composition.

[0009] To achieve the above object, the technical solution of the present invention is as follows:

[0010] The invention provides an insecticide composition, which consists of 0.1 to 70 parts of fluoxetine and 0.1 to 70 parts of spirobudic acid in parts by weight.

[0011] Preferably, the composition consists of 0.5 to 60 parts of fluoxetine and 0.5 to 60 parts of spirobudic acid in parts by weight.

[0012] Preferably, the composition consists of 1 to 60 parts of fluoxetine and 1 to 30 parts of spirobudic acid in parts by weight.

[0013] In a specific embodiment provided by the present invention, the composition consists of 11 parts of fluoxetine and 7 parts of spirobudic acid in parts by weight.

[0014] In another specific embodiment provided by the present invention, the composition consists of 23 parts of fluoxetine and 14 parts of spirobudic acid in parts by weight.

[0015] In another specific embodiment provided by the present invention, the composition consists of 40 parts of fluoxetine and 25 parts of spirobudic acid in parts by weight.

[0016] The present invention also provides an insecticide, comprising the insecticide composition of the present invention and an adjuvant acceptable in pesticides.

[0017] Preferably, the pesticide is in the form of a water suspension, water dispersible granules or dispersible oil suspension, but the form is not limited thereto.

[0018] Preferably, the components and weight percentages of the aqueous suspension are as follows: 0.1-70% of fluoxetine, 0.1-70% of spirobudic acid, 1-12% of wetting agent, 1-8% of thickener, 1-14% of dispersant, 1-6% of antifreeze agent, and water is added to 100%.

[0019] Preparation method: First, dilute spirobudic acid diester with water, then add fluoxetine to prepare a mixed solution, and then add dispersants, wetting agents, antifreeze agents and other additives. After grinding, mix with a thickener, and mix at a high speed to obtain a water suspension formulation.

[0020] More preferably, the components and weight percentages of the aqueous suspension are as follows: 1-60% of fluoxetine, 1-45% of spirobudic acid, 1-12% of wetting agent, 1-8% of thickener, 1-14% of dispersant, 1-6% of antifreeze agent, and water is added to 100%.

[0021] Preparation method: First, dilute spirobudic acid diester with water, then add fluoxetine to prepare a mixed solution, and then add dispersants, wetting agents, antifreeze agents and other additives. After grinding, mix with a thickener, and mix at a high speed to obtain a water suspension formulation.

[0022] Preferably, the components and weight percentages of the water dispersible granules are as follows: 0.1-70% of fluoxetine, 0.1-70% of spirobudic acid, 1-8% of disintegrant, 1-11% of dispersant, 1-18% of wetting agent, and the filler is supplemented to 100%.

[0023] Preparation method: First, dilute spirobudic acid diester with water, then add fluoxetine to prepare a mixed solution, and then add an auxiliary agent. After crushing with a jet mill and granulating, a water-dispersible granule dosage form can be obtained.

[0024] More preferably, the components and weight percentages of the water dispersible granules are as follows: 1-60% of fluoxetine, 1-45% of spirobudic acid, 1-8% of disintegrant, 1-11% of dispersant, 1-18% of wetting agent, and the filler is supplemented to 100%.

[0025] Preparation method: First, dilute spirobudic acid diester with water, then add fluoxetine to prepare a mixed solution, and then add an auxiliary agent. After crushing with a jet mill and granulating, a water-dispersible granule dosage form can be obtained.

[0026] Preferably, the components and weight percentages of the dispersible oil suspension formulation are as follows: 0.1-70% of fluoxetine, 0.1-70% of spirobudic acid, 1-7% of thickener, 1-17% of stabilizer, 1-14% of dispersant, 1-6% of emulsifier, 0-6% of defoamer, 1-5% of antifreeze, and vegetable oil is supplemented to 100%.

[0027] Preparation method: fluoxetine, spirobudic acid, stabilizer, dispersant, emulsifier, thickener, defoamer, antifreeze agent and vegetable oil are mixed, dispersed by high-speed shearing, and then sand-milled by a sand mill.

[0028] More preferably, the components and weight percentages of the dispersible oil suspension formulation are as follows: 1-70% of fluoxetine, 1-70% of spirobudic acid, 1-7% of thickener, 1-18% of stabilizer, 1-16% of dispersant, 1-8% of emulsifier, 0-8% of defoamer, 1-6% of antifreeze agent, and vegetable oil is supplemented to 100%.

[0029] Preparation method: fluoxetine, spirobudic acid, stabilizer, dispersant, emulsifier, thickener, defoamer, antifreeze agent and vegetable oil are mixed, dispersed by high-speed shearing, and then sand-milled by a sand mill.

[0030] The wetting agent is one or more of Terspense 4896, triethanolamine lauryl sulfate, monoethanolamine lauryl sulfate, sodium dodecyl sulfate, Morwet EFW, sodium dodecylbenzene sulfonate, butylnaphthalenesulfonic acid, fatty alcohol polyoxyethylene ether, polyethylene glycol 200, NP-10, Disperso BB4, Multiwe 8269, etc.

[0031] The above dispersant is one or more of Morwet D-425, sodium lignin sulfonate, Atlo 4913, Terspense 2500, AtlasG-5002L, Atlas G-5000, dispersant NNO, naphthalenesulfonate, and Dispersol PSR 19 polycarboxylate dispersants.

[0032] The above-mentioned disintegrant can be one or more of wgwin D800, inorganic salt (sodium sulfate, etc.), sodium carboxymethyl cellulose, bentonite, etc.

[0033] The filler is one or more of kaolin, talc, diatomaceous earth, bentonite and clay.

[0034] The antifreeze agent mentioned above is one or more of ethylene glycol, glycerol, propylene glycol, glycerol, etc.

[0035] The above-mentioned thickener is one or more of xanthan gum, magnesium aluminum silicate, polyethylene glycol, carboxymethyl cellulose, carboxyethyl cellulose and the like.

[0036] The stabilizer is one or more of epichlorohydrin, epoxy soybean oil, sodium benzoate and the like.

[0037] The above-mentioned emulsifier is one or more of the emulsifiers suitable for vegetable oil, such as YUS-110 emulsifier and methyl oleate.

[0038] The above-mentioned defoaming agent is selected from: one or more of silicone oil, silicone compounds, etc.

[0039] The present invention also provides the use of the insecticide composition in preventing and controlling pests in cotton, eggplant, corn and the like.

[0040] The present invention provides an insecticide composition and an insecticide. The composition is composed of 0.1 to 70 parts of fluoxazolidinone and 0.1 to 70 parts of spirobudic acid in parts by weight. Compared with the prior art, the insecticide composition and the insecticide of the present invention have the following beneficial effects:

[0041] 1) The insecticide composition of the present invention comprises fluoxathiaceae and spirobudicola as active ingredients, fluoxathiaceae is an isoxazole insecticide, and spirobudicola belongs to a tetronix ester acaricide. The compounding of different types of ingredients has obvious synergistic effects on cotton bollworm, Turkestan spider mite, two-spotted spider mite, truncate spider mite, corn borer and thrips through a variety of different modes of action, and is significantly better than the effects of fluoxathiaceae single agent, spirobudicola single agent and other preparations. While effectively reducing the dosage of fluoxathiaceae and spirobudicola, it can expand the target range of the two insecticides, reduce the number of applications, and reduce the production cost.

[0042] 2) Aqueous suspension concentrates have many advantages, such as: ① With water as the matrix, they can be quickly dispersed in water, are environmentally friendly, safe to use, and have low cost; ② have no flash point, are safe to store and transport, and have high bioavailability.

[0043] 3) Water-dispersible granules have many advantages, such as: ① They do not contain organic solvents and are safe for operators and the environment; ② The product is dust-free during packaging, storage and use, reducing the harm of dust to the human body; ③ The dosage form has good fluidity, does not stick, agglomerate or stick to the wall, and the packaging is recyclable; ④ The content of active ingredients is high.

[0044] 4) Oil suspension has many advantages, such as: the dilution carrier is an environmentally friendly oil, has good adhesion and spreadability, and strong resistance to rain erosion.

[0045] 5) Reduce the number of pests and reduce the degree of damage to crops.

[0046] 6) It is very safe for mammals, aquatic organisms, beneficial organisms, natural enemies and other non-target organisms, and is highly effective and low-risk. DETAILED DESCRIPTION

[0047] The present invention discloses an insecticide composition and an insecticide, and those skilled in the art can refer to the content of this article and appropriately improve the process parameters to achieve the same. It should be particularly noted that all similar substitutions and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the methods and applications described herein without departing from the content, spirit and scope of the present invention to implement and apply the technology of the present invention.

[0048] The raw materials or adjuvants used in the insecticide composition and the insecticide provided by the invention can be purchased from the market.

[0049] The present invention will be further described below in conjunction with embodiments:

[0050] Example 1: 18% aqueous suspension

[0051] Formula (weight ratio): fluoxetine 11%, spirobudic acid 7%, dispersant Atlo 4913 17%, DispersoBB 47%, ethylene glycol 6%, xanthan gum 6%, water to make up 100%.

[0052] Preparation method: First, dilute spirobudic acid diester with water, then add fluoxetine to prepare a mixed solution, and then add auxiliary dispersant Atlo 4913, ethylene glycol and Disperso BB4, grind and mix with xanthan gum, and mix at high speed to obtain a water suspension formulation.

[0053] Example 2: 37% dispersible oil suspension

[0054] Formula (weight ratio): 23% of fluoxetine amide, 14% of spirobudic acid, 10% of sodium lignin sulfonate, 12% of YUS-110 emulsifier, 4% of ethylene glycol, 2% of xanthan gum, 3% of epoxidized soybean oil, 2% of silicone oil, and vegetable oil (soybean oil) is supplemented to 100%.

[0055] Preparation method: weigh fluoxetine and spirobudic acid into a container, add an emulsifier, a dispersant and soybean oil, mix and disperse at high speed, then grind with a sand mill, and filter to obtain a dispersible oil suspension formulation.

[0056] Example 3: 65% water dispersible granules

[0057] Formula (weight ratio): fluoxetine 40%, spirobudic acid 25%, sodium lauryl sulfate 6%, SP-2836 polycarboxylate dispersant 7%, sodium sulfate 3%, kaolin 8%, diatomaceous earth makes up 100%.

[0058] Preparation method: First, dilute spirobudic acid with water, then add fluoxetine to prepare a mixed solution, and then add an auxiliary agent. After crushing with an air flow crusher and granulating, a water-dispersible granule dosage form can be obtained.

[0059] Test example: Field test 1 Field efficacy test of fluoxetine amide and spiroclofenac combination in controlling cotton bollworm

[0060] 1. Test reagents

[0061] The tested agents were the insecticide compositions of Examples 1-3 of the present invention (test groups 1-3), 10% fluazifop SC (control group 1), and 24% spirobudic acid dienolide SC (control group 2).

[0062] 2. Experimental Design

[0063] This experiment was carried out at the Bozhou Experimental Station of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences in Xinjiang. The pesticide application equipment was a Spanish backpack manual sprayer with a spray volume of 600L·hm -2 The specific situation of the test site is as follows:

[0064] The cotton variety is China Cotton Research Institute 49, which was sown on April 28, 2024, covered with plastic film, and planted at a density of 123,000 plants / hm2. -2 The row spacing is 76 cm, the plant spacing is 9 cm, the application date is July 10, 2024, and there are 4 replicates for each treatment. At this time, there are 10 to 20 larvae of cotton bollworm per 100 plants.

[0065] 3. Survey Content

[0066] In each plot, 25 representative cotton plants were selected from the middle 4 rows for fixed points and tagged. The base number before application was investigated on the day of application, and the residual insect quantity on the fixed cotton plants at each point was investigated 3d and 7d after application. The insect population reduction rate of each survey plot was calculated overall. The insect population reduction rate in the pesticide treatment group was compared with that in the blank control group, and the corrected control effect (%) was calculated.

[0067] 4. Calculation formula

[0068]

[0069]

[0070] 5. Test results

[0071] As can be seen from Table 1, spraying the following five treatments has different control effects on cotton bollworm. Among them, the dosage of the active ingredient in Example 2 of the present invention is lower than the sum of the dosage of the active ingredients in Control 1 and Control 2, and the corrected control efficacy of Example 2 at 3d and 7d after the application is 85.0% and 94.1% respectively. The corrected control efficacy of Example 2 at 3d and 7d after the application is higher than the theoretical efficacy of the corresponding other treatments. Therefore, the combination of fluoxetine and spiroclofen has a significant synergistic effect, and no phytotoxicity of the combined agent to cotton was found during the test.

[0072] Table 1 Field test efficacy of fluoxetine amide and spiroclofenac combination in controlling cotton bollworm

[0073]

[0074] Note: “-” means “blank” or “no calculation required”; the same below.

[0075] Test example: Field test 2: Field efficacy test of the combination of fluoxetine and spiroclofenicol to control Turkestan spider mites

[0076] 1. Test reagents

[0077] The tested agents were the insecticide compositions of Examples 1-3 of the present invention (test groups 1-3), 10% fluazifop SC (control group 1), and 24% spirobudic acid dienolide SC (control group 2).

[0078] 2. Experimental Design

[0079] This experiment was carried out at the Bozhou Experimental Station of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences in Xinjiang. The pesticide application equipment was a Spanish backpack manual sprayer with a spray volume of 600L·hm -2 The specific situation of the test site is as follows:

[0080] The cotton variety is China Cotton Research Institute 49, which was sown on April 28, 2024, covered with plastic film, and planted at a density of 123,000 plants / hm2. -2 The row spacing is 76 cm, the plant spacing is 9 cm, and the application date is June 23, 2024, with 4 replicates per treatment. This is the initial peak period of the occurrence of Turkestan spider mites.

[0081] 3. Survey Content

[0082] In each plot, 25 leaves of representative cotton plants were selected from the middle 4 rows for fixed points and labeled. The base number before application was investigated on the day of application, and the residual insect quantity on the cotton leaves at each point was investigated 3d and 7d after application. The insect population reduction rate of each survey plot was calculated overall. The insect population reduction rate in the treatment group was compared with that in the blank control group, and the corrected control effect (%) was calculated.

[0083] 4. Calculation formula

[0084]

[0085] 5. Test results

[0086] As can be seen from Table 2, spraying the following five treatments has different control effects on Turkestan spider mites. Among them, the amount of the effective ingredient in Example 2 of the present invention is lower than the sum of the effective ingredient amounts in Control 1 and Control 2, and the corrected control efficacy of Example 2 at 3d and 7d after the drug application is 88.8% and 96.9% respectively. The corrected control efficacy of Example 2 at 3d and 7d after the drug application is higher than the theoretical efficacy of the corresponding other treatments. Therefore, the combination of fluoxetine and spirobudic acid has a significant synergistic effect, and no phytotoxicity of the combined agent to cotton was found during the test.

[0087] Table 2 Field test efficacy of fluoxetine amide and spiroclofenac combination in controlling Turkestan spider mites

[0088]

[0089] Test example: Field test 3: Field efficacy test of the combination of fluoxetine amide and spiroclofenac for controlling spider mites

[0090] 1. Test reagents

[0091] The tested agents were the insecticide compositions of Examples 1-3 of the present invention (test groups 1-3), 10% fluazifop SC (control group 1), and 24% spirobudic acid dienolide SC (control group 2).

[0092] 2. Experimental Design

[0093] This experiment was carried out at the experimental farm of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences in Baibi Town, Anyang County, Henan Province. The pesticide application equipment was a Singapore Linong backpack manual sprayer with a spray volume of 900L·hm-2 The specific situation of the test site is as follows:

[0094] The eggplant variety is Da Hong Pao, which was transplanted and planted on March 28, 2024, covered with plastic film, and the planting density is 52,500 plants / hm2 -2 The application date was May 26, 2024, with 4 replicates for each treatment.

[0095] 3. Survey Content

[0096] Randomly sample 5 points in each plot, investigate 2 plants at each point, and select 2 leaves with mites in the middle of each plant for fixed plant and leaf investigation. Investigate the pre-drug base number on the day of application, and investigate the residual insect quantity on eggplant leaves at each point 3d and 7d after application. Calculate the overall insect population reduction rate of each survey plot. Compare the insect population reduction rate in the drug treatment group with that in the blank control group, and calculate the corrected control effect (%).

[0097] 4. Calculation formula

[0098]

[0099] 5. Test results

[0100] As can be seen from Table 3, spraying the following five treatments has different control effects on two-spotted spider mites. Among them, the amount of the effective ingredient in Example 2 of the present invention is lower than the sum of the effective ingredient amounts in Control 1 and Control 2, and the corrected control efficacy of Example 2 at 3d and 7d after the drug application is 88.4% and 93.8% respectively. The corrected control efficacy of Example 2 at 3d and 7d after the drug application is higher than the theoretical efficacy of the corresponding other treatments. Therefore, the combination of fluoxetine and spiroclofen has a significant synergistic effect, and no phytotoxicity of the combined agent to eggplant was found during the test.

[0101] Table 3 Field test efficacy of fluoxetine amide and spiroclofenac combination in controlling Tetranychus urticae

[0102]

[0103] Test example: Field test 4: Field efficacy test of the combination of fluoxetine amide and spiroclofenicol for controlling truncate spider mites

[0104] 1. Test reagents

[0105] The tested agents were the insecticide compositions of Examples 1-3 of the present invention (test groups 1-3), 10% fluazifop SC (control group 1), and 24% spirobudic acid dienolide SC (control group 2).

[0106] 2. Experimental Design

[0107] This experiment was carried out at the experimental farm of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences in Baibi Town, Anyang County, Henan Province. The spraying equipment was a Spanish backpack manual sprayer with a spray volume of 600L·hm -2 The specific situation of the test site is as follows:

[0108] The cotton variety is China Cotton Research Institute 49, which was sown on April 24, 2024, with a planting density of 55,000 plants / hm2. -2 The row spacing is 80 cm, the application date is June 23, 2024, and there are 4 replicates per treatment. This is the initial peak period of the occurrence of truncate spider mites.

[0109] 3. Survey Content

[0110] In each plot, 25 leaves of representative cotton plants were selected from the middle 4 rows for fixed points and labeled. The base number before application was investigated on the day of application, and the residual insect quantity on the cotton leaves at each point was investigated 3d and 7d after application. The insect population reduction rate of each survey plot was calculated overall. The insect population reduction rate in the treatment group was compared with that in the blank control group, and the corrected control effect (%) was calculated.

[0111] 4. Calculation formula

[0112]

[0113] 5. Test results

[0114] As can be seen from Table 4, spraying the following five treatments has different control effects on truncate spider mites. Among them, the amount of the effective ingredient in Example 2 of the present invention is lower than the sum of the effective ingredient amounts in Control 1 and Control 2, and the corrected control efficacy of Example 2 at 3d and 7d after the drug application is 92.0% and 95.1% respectively. The corrected control efficacy of Example 2 at 3d and 7d after the drug application is higher than the theoretical efficacy of the corresponding other treatments. Therefore, the combination of fluoxetine and spirobudic acid has a significant synergistic effect, and no phytotoxicity of the combined agent to cotton was found during the test.

[0115] Table 4 Field test efficacy of fluoxetine amide and spiroclofenac combination in controlling Turkestan spider mites

[0116]

[0117] Test example: Field test 5: Field efficacy test of fluoxetine amide and spiroclofenac combination in controlling corn borer

[0118] 1. Test reagents

[0119] The tested agents were the insecticide compositions of Examples 1-3 of the present invention (test groups 1-3), 10% fluazifop SC (control group 1), and 24% spirobudic acid dienolide SC (control group 2).

[0120] 2. Experimental Design

[0121] This experiment was carried out at the experimental farm of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences in Baibi Town, Anyang County, Henan Province. The spraying equipment was a Spanish backpack manual sprayer with a spray volume of 600L·hm -2 The specific situation of the test site is as follows:

[0122] The corn variety is Anyu 107, which was sown on May 22, 2024, with a planting density of 75,000 plants / hm2. -2 The application date is June 28, 2024, with 4 replicates in each plot. This is the peak hatching period for corn borers.

[0123] 3. Survey Content

[0124] In each plot, 25 representative corn plants were selected from the middle 4 rows for fixed points and tagged. The base number before application was investigated on the day of application, and the residual insect quantity on the corn plants at each point was investigated 3d and 7d after application. The insect population reduction rate of each survey plot was calculated overall. The insect population reduction rate in the pesticide treatment group was compared with that in the blank control group, and the corrected control effect (%) was calculated.

[0125] 4. Calculation formula

[0126]

[0127] 5. Test results

[0128] As can be seen from Table 5, spraying the following five treatments has different control effects on corn borers. Among them, the amount of the effective ingredient in Example 2 of the present invention is lower than the sum of the effective ingredient amounts in Control 1 and Control 2, and the corrected control effects of Example 2 at 3d and 7d after the application are 86.5% and 95.8% respectively. The corrected control effects of Example 2 at 3d and 7d after the application are higher than the theoretical efficacy of the corresponding other treatments. Therefore, the combination of fluoxetine and spirobudic acid has a significant synergistic effect, and no phytotoxicity to corn was found in the combined agent during the test.

[0129] Table 5 Field test efficacy of fluoxetine amide and spiroclofenac combination in controlling corn borer

[0130]

[0131] Test example: Field test of the efficacy of the combination of 6-fluoxazolidinone and spiroclofenac in controlling thrips

[0132] The tested agents were the insecticide compositions of Examples 1-3 of the present invention (test groups 1-3), 10% fluazifop SC (control group 1), and 24% spirobudic acid dienolide SC (control group 2).

[0133] 2. Experimental Design

[0134] This experiment was carried out at the Bozhou Experimental Station of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences in Xinjiang. The pesticide application equipment was a Spanish backpack manual sprayer with a spray volume of 600L·hm -2 The specific situation of the test site is as follows:

[0135] The cotton variety is China Cotton Research Institute 49, which was sown on April 28, 2024, covered with plastic film, and planted at a density of 123,000 plants / hm2. -2 , row spacing 76cm, plant spacing 9cm, application date is August 23, 2024, with 4 replicates per treatment, which is the initial peak period of thrips occurrence.

[0136] 3. Survey Content

[0137] In each plot, 25 representative cotton plants were selected from the middle 4 rows for fixed points and tagged. The base number before application was investigated on the day of application, and the residual insect quantity on the fixed cotton plants at each point was investigated 3d and 7d after application. The insect population reduction rate of each survey plot was calculated overall. The insect population reduction rate in the pesticide treatment group was compared with that in the blank control group, and the corrected control effect (%) was calculated.

[0138] 4. Calculation formula

[0139]

[0140]

[0141] 5. Test results

[0142] As can be seen from Table 6, different control effects on thrips were achieved by spraying the following five treatments. The dosage of the active ingredient in Example 2 of the present invention was lower than the sum of the dosage of the active ingredients in Control 1 and Control 2, and the corrected control efficacy of Example 2 at 3d and 7d after the application was 92.6% and 96.9%, respectively. The corrected control efficacy of Example 2 at 3d and 7d after the application was higher than the theoretical efficacy of the corresponding other treatments. Therefore, the combination of fluoxetine and spiroclofen has a significant synergistic effect, and no phytotoxicity of the combined agent to cotton was found during the test.

[0143] Table 6 Field test efficacy of fluoxetine amide and spiroclofenac combination in controlling cotton thrips

[0144]

[0145] Test example: Field test 7 Field efficacy test of fluoxetine amide and spiroclofenac combination in controlling cotton aphids

[0146] The tested agents were the insecticide compositions of Examples 1-3 of the present invention (test groups 1-3), 10% fluazifop SC (control group 1), and 24% spirobudic acid dienolide SC (control group 2).

[0147] 2. Experimental Design

[0148] This experiment was carried out at the Bozhou Experimental Station of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences in Xinjiang. The pesticide application equipment was a Spanish backpack manual sprayer with a spray volume of 600L·hm -2 The specific situation of the test site is as follows:

[0149] The cotton variety is China Cotton Research Institute 49, which was sown on April 28, 2024, covered with plastic film, and planted at a density of 123,000 plants / hm2. -2 The row spacing is 76 cm, the plant spacing is 9 cm, and the application date is July 23, 2024. There are 4 replicates for each treatment. This is the initial peak period of cotton aphid occurrence.

[0150] 3. Survey Content

[0151] In each plot, 25 representative cotton plants were selected from the middle 4 rows for fixed points and tagged. The base number before application was investigated on the day of application, and the residual insect quantity on the fixed cotton plants at each point was investigated 3d and 7d after application. The insect population reduction rate of each survey plot was calculated overall. The insect population reduction rate in the pesticide treatment group was compared with that in the blank control group, and the corrected control effect (%) was calculated.

[0152] 4. Calculation formula

[0153]

[0154] 5. Test results

[0155] As can be seen from Table 7, spraying the following five treatments has different control effects on cotton aphids. Among them, the dosage of the effective ingredient in Example 2 of the present invention is lower than the sum of the dosage of the effective ingredients in Control 1 and Control 2, and the corrected control efficacy of Example 2 at 3d and 7d after the application is 86.4% and 95.7% respectively. The corrected control efficacy of Example 2 at 3d and 7d after the application is higher than the theoretical efficacy of the corresponding other treatments. Therefore, the combination of fluoxetine and spiroclofen has a significant synergistic effect, and no phytotoxicity to cotton was found in the combined agent during the test.

[0156] Table 7 Field test efficacy of fluoxetine amide and spiroclofenac combination in controlling cotton aphids

[0157]

[0158] Therefore, the combination of fluoxetine and spirobudic acid has a significant synergistic effect in controlling pests such as cotton bollworm, Turkestan spider mite, two-spotted spider mite, truncate spider mite, corn borer, thrips and aphids.

[0159] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. An insecticidal composition, characterized in that: In parts by weight, the composition consists of 0.1 to 70 parts of fluoxetine and 0.1 to 70 parts of spirobudic acid.

2. An insecticidal composition according to claim 1, characterized in that: In parts by weight, the composition consists of 0.5 to 60 parts of fluoxetine and 0.5 to 60 parts of spirobudic acid.

3. The insecticidal composition according to claim 1, characterized in that: In parts by weight, the composition consists of 1 to 60 parts of fluoxetine and 1 to 30 parts of spirobudic acid.

4. An insecticidal composition according to any one of claims 1 to 3, characterized in that: In parts by weight, the composition consists of 11 parts of fluoxetine and 7 parts of spirobudic acid.

5. An insecticide, characterized in that: The invention comprises the insecticidal composition according to any one of claims 1 to 4 and an adjuvant which is acceptable in pesticides.

6. The insecticide according to claim 5, characterized in that: The formulation of the insecticide is a water suspension, a water dispersible granule or a dispersible oil suspension.

7. An insecticide according to claim 6, characterized in that: The components and weight percentages of the aqueous suspension are as follows: 0.1% to 70% of fluoxetine, 0.1% to 70% of spiroclofen, 1% to 8% of thickener, 1% to 12% of wetting agent, 1% to 6% of antifreeze agent, 1% to 14% of dispersant, and water is added to 100%; The preparation method is as follows: dilute spiroclofenac with water, add fluoxetine to obtain a mixed solution, add a dispersant, an antifreeze agent and a wetting agent, grind the mixture, mix it with a thickener, and mix it at a high speed by shearing.

8. The insecticide according to claim 6, characterized in that: The components and weight percentages of the water dispersible granules are as follows: 0.1% to 70% of fluoxetine amide, 0.1% to 70% of spirobudic acid, 1% to 11% of dispersant, 1% to 18% of wetting agent, 1% to 8% of disintegrant, and filler is added to 100%; The preparation method is as follows: dilute spiroclofenac with water, add fluoxetine to obtain a mixed solution, then add a dispersant, a wetting agent, a disintegrant and a filler, and grind the mixture with a jet mill to form granules.

9. The insecticide according to claim 6, characterized in that: The components and weight percentages of the dispersible oil suspension formulation are as follows: 0.1% to 70% fluoxetine, 0.1% to 70% spiroclofen, and a stabilizer. 1%~18%, dispersant 1%~16%, emulsifier 1%~8%, thickener 1%~5%, defoamer 0~8%, antifreeze 1%~6%, vegetable oil to 100%; The preparation method comprises the following steps: mixing fluoxazolidinone, spirobudic acid diester, a stabilizer, a dispersant, an emulsifier, a thickener, a defoamer, an antifreeze agent and vegetable oil, dispersing the mixture by high-speed shearing, and then sand-grinding the mixture with a sand mill.

10. An insecticide according to any one of claims 7 to 9, characterized in that: The wetting agent is one or more of Terspense 4896, triethanolamine lauryl sulfate, monoethanolamine lauryl sulfate, sodium dodecyl sulfate, MorwetEFW, sodium dodecylbenzene sulfonate, butylnaphthalenesulfonic acid, fatty alcohol polyoxyethylene ether, polyethylene glycol 200, NP-10, DispersoBB4, Multiwe 8269, etc.; The dispersant is one or more of Morwet D-425, sodium lignin sulfonate, Atlo 4913, Terspense 2500, Atlas G-5002L, Atlas G-5000, dispersant NNO, naphthalene sulfonate, Dispersol PSR 19 polycarboxylate dispersants; The disintegrant is one or more of wgwin D800, inorganic salt (sodium sulfate, etc.), sodium carboxymethyl cellulose, bentonite, etc.; The filler is one or more of kaolin, talc, diatomaceous earth, bentonite, and clay; the antifreeze agent is one or more of ethylene glycol, glycerol, propylene glycol, glycerol, etc.; The thickener is one or more of xanthan gum, magnesium aluminum silicate, polyethylene glycol, carboxymethyl cellulose, and carboxyethyl cellulose; The stabilizer is one or more of epichlorohydrin, epoxy soybean oil, and sodium benzoate; the emulsifier is one or two of YUS-110 emulsifier and methyl oleate; The defoaming agent is one or both of silicone oil and silicone compounds.

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