Synergistic bactericidal composition and application thereof

Abstract

The invention discloses a synergistic bactericidal composition and application thereof. The composition provided by the invention comprises the bacillus amyloliquefaciens ZJLMBA1908 strain fermentation liquor and the chemical bactericide, has a synergistic bactericidal effect through combined use of bacteria and drugs, can significantly improve the inhibition effect on pathogenic fungus Arthrosporium neodarksonii, and has a significant control effect on the dragon fruit canker, and the effect of the composition is significantly superior to that of a single strain or a single bactericide. The composition provided by the invention reduces the dosage of each component, further reduces the concentration of the bactericide, avoids the drug resistance and environmental pollution, can be used as an environment-friendly, decrement and synergistic novel bactericidal product to better prevent and treat the dragon fruit canker, improves the prevention and treatment efficiency of the bactericide on fungal diseases, and has a good application prospect in prevention and treatment of the dragon fruit canker. The usage amount of the chemical bactericide is reduced, the bactericidal spectrum of the copper-containing bactericide can be further expanded, and technical support is provided for drug screening of plant diseases such as dragon fruit canker and'combined use of bacteria and drugs'.

Description

Technical Field

[0001] The present invention belongs to the technical field of microbial preparations and agricultural disease prevention and control, and more specifically, relates to a synergistic bactericidal composition and its application. Background Art

[0002] In agricultural ecosystems, crops may develop plant diseases due to the stress of some biological or abiotic factors, causing abnormal physiological activities or growth and development, thus affecting their yield, quality and utilization value. The factors that lead to plant diseases are very complex, but among them, infectious plant diseases caused by pathogenic microorganisms are the main factor causing crop yield losses and deterioration in agricultural product quality worldwide. The occurrence of these infectious diseases seriously threatens agricultural production, national economic construction and food security. Efficient and safe prevention and control measures are an important way to control the occurrence and spread of plant diseases and reduce crop losses.

[0003] At present, the most important means of controlling infectious diseases is to use chemical fungicides, such as carbendazim, azoxystrobin, methyl thiophanate, thiabendazole, prochloraz, etc. However, long-term and large-scale use of fungicides will induce drug resistance in pathogens, reduce their control effect, and there are also a series of problems such as excessive pesticide residues, harm to human and animal health, and environmental pollution. Therefore, it is very necessary to seek a new control method that is safe, efficient, and can replace or reduce the use of chemical fungicides.

[0004] With the development of biological control agents, more and more plant diseases can be controlled by microbial preparations. Microbial agents have the advantages of not polluting the environment, being non-toxic to humans and animals, and having no side effects on plants. They can also avoid the environmental pollution and ecological damage that may be caused by chemical fungicides. However, the control effect of existing biological control bacteria is generally not as significant as that of chemical fungicides, and the control effect is easily affected by environmental conditions; and some chemical fungicides also have different control effects on different plant pathogens. For example, copper fungicides such as Spring Thunder · Quinoline Copper Suspension and Spring Thunder · Copper King Wettable Powder are mainly used for bacterial disease control, and often show selectivity and limitations in the control of fungal diseases, and the control effect is poor. The pitaya canker is caused by Neoscytalidium dimidiatum, which infects the fleshy stem and fruit of pitaya. It is a fungal disease and one of the main diseases in pitaya. It is extremely destructive and the disease develops very quickly. If it cannot be controlled in time, it will cause the stems of the whole garden to rot and the fruits to rot, which will seriously damage the growth of the plants and cause premature aging of the plants and even the destruction of the whole garden. Because the existing existing biocontrol bacteria control effect does not meet the control requirements in agricultural production, and most of the chemical fungicides have poor control effects on pitaya canker, excessive use easily causes the drug resistance of pathogens and negative impacts on the environment. Therefore, there is still a lack of drugs or antibacterial products that can effectively control pitaya canker. Summary of the invention

[0005] The technical problem to be solved by the present invention is to overcome the problem that the control effect of existing biocontrol bacteria cannot meet the control requirements in agricultural production, and most chemical fungicides have poor control effects on pitaya canker disease, and excessive use can easily cause drug resistance of pathogens and negative impact on the environment, thereby providing a synergistic bactericidal composition and its application.

[0006] The first object of the present invention is to provide a synergistic fungicidal composition.

[0007] The second object of the present invention is to provide the use of the composition.

[0008] The third object of the present invention is to provide an antibacterial product.

[0009] The fourth object of the present invention is to provide a method for preventing and treating pitaya canker

[0010] The fourth object of the present invention is to provide the use of the fermentation broth of Bacillus amyloliquefaciens ZJLMBA1908 strain as a synergist.

[0011] The above-mentioned purpose of the present invention is achieved through the following technical solutions:

[0012] The invention provides a composition, comprising a fermentation broth of a Bacillus amyloliquefaciens ZJLMBA1908 strain and a chemical fungicide; the volume ratio of the fermentation broth of the ZJLMBA1908 strain to the chemical fungicide is (1-3):1; the ZJLMBA1908 strain has been deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration on November 5, 2019, with a deposit number of CGMCC No.18907; the chemical fungicide is selected from one or more of a benzoyl-azoxystrobin suspension, a pyraclostrobin-azoxystrobin suspension, a pyraclostrobin-quinoline copper suspension, and a pyraclostrobin-copper wettable powder.

[0013] The present invention provides a synergistic antibacterial biological-chemical sterilization composition, including a fermentation liquid of bacillus amyloliquefaciens and a bactericide, through the combination of fungi and drugs, the fungicide and the microbial agent are compounded to have a significant synergistic antibacterial effect, significantly improve the inhibitory effect on the pathogenic fungus Neoscytalidium dimidiatum, inhibit the mycelial growth and spore germination of pitaya canker pathogens, and have a significant control effect on pitaya canker, and its effect is significantly better than that of using strains or bactericides alone; combining them and not only can reduce the dosage of fungicides and bactericides, but also can further reduce the concentration of bactericides, thereby reducing the drug resistance and environmental pollution caused by excessive use of chemical bactericides, and achieving a good inhibitory effect. Therefore, the drug-bacteria composition provided by the present invention can be better used for the prevention and treatment of pitaya canker during pitaya planting as an environmentally friendly, reduced-quantity, synergistic, and novel sterilization product.

[0014] Preferably, the fermentation broth is a sterile fermentation supernatant, and its concentration is not less than 100 μL / mL.

[0015] Preferably, the concentration of the chemical fungicide is ≥ 0.0625 mg / L.

[0016] The invention provides application of the composition in preventing and treating pitaya ulcer disease.

[0017] The present invention provides application of the composition in inhibiting Neoscytalidium dimidiatum.

[0018] The present invention provides application of the composition in preparing antibacterial products.

[0019] Preferably, the product can inhibit Neopyrocoelenteroides and plant diseases caused by it.

[0020] The invention provides an antibacterial product comprising the composition.

[0021] The invention also provides a method for preventing and treating pitaya ulcer disease, which comprises using the composition or product to treat pitaya.

[0022] Preferably, the dragon fruit is sprayed with the composition.

[0023] In addition, in the composition provided by the present invention, the copper preparations of spring thunder · quinoline copper suspension and spring thunder · copper WP in the compound fungicide of ZJLMBA1908 strain fermentation liquid have the best effect; since the copper preparation fungicide itself has a poor effect on fungi, compounding it with ZJLMBA1908 strain fermentation liquid significantly improves the antibacterial effect of the copper preparation, can better inhibit mycelial growth and spore germination, and enhances the effect of the copper preparation. Based on this, the fermentation liquid of Bacillus amyloliquefaciens ZJLMBA1908 strain can be used as a synergist to enhance the inhibitory effect of copper preparation fungicides on fungi, broaden the application of copper preparations in fungal disease prevention and control, and also improve the control efficiency of copper preparations on fungal diseases, providing more methods and products for the prevention and control of pitaya canker during pitaya planting, and has a wide range of application value.

[0024] The invention provides application of fermentation liquid of Bacillus amyloliquefaciens ZJLMBA1908 strain as a synergist in improving the inhibitory effect of a copper preparation fungicide on fungi.

[0025] Preferably, the fungus is Arthrospora neopyri.

[0026] Preferably, the inhibitory effect refers to the inhibition of hyphae growth and / or spore germination.

[0027] The present invention has the following beneficial effects:

[0028] The present invention provides a synergistic antibacterial biological-chemical sterilization composition, including a fermentation liquid of bacillus amyloliquefaciens and a bactericide, through the combination of fungi and drugs, the fungicide and the microbial agent are compounded to have a synergistic antibacterial effect, can significantly improve the inhibitory effect on the pathogenic fungus Neoscytalidium dimidiatum (Neoscytalidium dimidiatum), can inhibit the mycelial growth and spore germination of pitaya canker pathogens, and has a significant control effect on pitaya canker, and its effect is significantly better than that of using strains or bactericides alone, and the combination thereof can not only reduce the dosage of bactericides and bactericides, but also further reduce the concentration of bactericides, thereby reducing the drug resistance and environmental pollution caused by excessive use of chemical bactericides, and achieving a good inhibitory effect. Therefore, the drug-bacteria composition provided by the present invention can be better used for the prevention and treatment of pitaya canker during pitaya planting as an environmentally friendly, reduced, synergistic, and novel sterilization product. Among them, the copper preparations Chunlei·Quinoline Copper Suspension and Chunlei·Royal Copper Wettable Powder among the compound fungicides have the best effect. They not only broaden the application of copper preparations in the prevention and control of fungal diseases, but also improve the prevention and control efficiency of copper preparations on fungal diseases. It also provides more methods and products for the prevention and control of pitaya canker during pitaya cultivation, and has wide application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1The invention discloses an inhibitory effect of the antibacterial composition on the mycelial growth of pitaya canker pathogen.

[0030] Figure 2 It is the inhibition rate of the antibacterial composition on the mycelial growth of pitaya canker pathogen.

[0031] Figure 3 The invention discloses an antibacterial composition for inhibiting the germination of spores of pitaya canker.

[0032] Figure 4 The present invention shows the preventive and therapeutic effect of the antibacterial composition on pitaya canker. DETAILED DESCRIPTION

[0033] The present invention is further described below in conjunction with the accompanying drawings and specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0034] Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

[0035] The culture medium formula used in the following examples is:

[0036] NA medium: 5.0 g beef extract, 10.0 g peptone, 5.0 g NaCl, 20.0 g agar, dilute to 1000 mL with distilled water, sterilize at 121°C for 20 min.

[0037] NA liquid culture medium: 5.0 g beef extract, 10.0 g peptone, 5.0 g NaCl, dilute to 1000 mL with distilled water, sterilize at 121°C for 20 min.

[0038] PDA solid culture medium: 200.0 g peeled potatoes, 20.0 g sucrose, 20.0 g agar, dilute to 1000 mL with distilled water, sterilize at 121°C for 20 min.

[0039] The Bacillus amyloliquefaciens ZJLMBA1908 strain used in the embodiment is the preliminary research result of the applicant's research team. It was deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration on November 5, 2019, with a deposit number of CGMCC No. 18907 and a deposit address of No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0040] 32.5% benzoyl·pyraclostrobin suspension concentrate (Syngenta Crop Protection Co., Ltd., pesticide batch number PD20150707), 29% pyraclostrobin·pyraclostrobin suspension concentrate (Syngenta Crop Protection Co., Ltd., pesticide batch number PD20170604), 30% pyraclostrobin·kresoxim-methyl suspension concentrate (Shanghai Yuelian Biotechnology Co., Ltd., pesticide batch number PD20180716), 45% Chunlei·quinoline copper suspension concentrate (Xingnong Pharmaceutical (China) Co., Ltd., pesticide batch number PD20181303) and 50% Chunlei·king copper wettable powder (Shouguang Chengxin Pesticide Co., Ltd., pesticide batch number PD20122093). All of the above agents were purchased from Zhongbao Green Agriculture Group, Institute of Plant Protection, Chinese Academy of Agricultural Sciences.

[0041] Example 1 Inhibitory effect on the mycelial growth of pitaya canker pathogen

[0042] 1. Preparation of sterile fermentation supernatant plates

[0043] The activated single colony of Bacillus amyloliquefaciens ZJLMBA1908 was inoculated into 50 mL NA liquid culture medium, and cultured at 37°C and 180 rpm in a shaking incubator for 24 h. The culture solution was centrifuged at 4°C and 13535×g for 10 min, the supernatant was collected, and filtered with a microporous filter membrane (pore size 0.22 μm) to obtain the sterile fermentation broth (CM) of the strain.

[0044] The prepared CM was then added to the PDA medium cooled to about 50°C at a ratio of 20 mL / 100 mL, and then poured into a sterilized culture dish to prepare a plate containing CM (the concentration of the sterile fermentation supernatant was 100 μL / mL).

[0045] 2. Preparation of fungicide plates

[0046] The 32.5% benzyl·azoxystrobin suspension, 29% pyraclostrobin·azoxystrobin suspension, 30% pyraclostrobin suspension, 45% pyraclostrobin·quinoline copper suspension and 50% pyraclostrobin·copper oxalate wettable powder were prepared into concentrations of 1.25, 2.5 and 5 mg / L respectively for standby use. The prepared agents were then added to the PDA culture medium cooled to about 50°C at a ratio of 20 mL / 100 mL, and then poured into a sterilized culture dish to prepare plates with fungicide concentrations of 0.25, 0.5 and 1 mg / L respectively.

[0047] 3. Preparation of biological agent-chemical fungicide combination plates

[0048] 32.5% benzoyl·pyraclostrobin suspension, 29% pyraclostrobin·pyraclostrobin suspension, 30% pyraclostrobin suspension, 45% pyraclostrobin·quinoline copper suspension and 50% pyraclostrobin·copper wang wettable powder were prepared to concentrations of 1.25, 2.5 and 5 mg / L respectively for use, and then CM (the concentration of sterile fermentation supernatant was 100 μL / mL) was mixed with chemical sterilization in a volume ratio of (1-3): 1, according to (10+10) mL / 100mL and (15+5)mL / 100mL were added into a PDA culture medium cooled to about 50°C, and then poured into a sterilized culture dish to prepare composition plates with fungicide concentrations of 0.125, 0.25 and 0.5 mg / L (CM: agent = 1:1), and composition plates with fungicide concentrations of 0.0625, 0.125 and 0.25 mg / L (CM: agent = 3:1), respectively.

[0049] 4. Determination of antibacterial rate

[0050] Use a 5mm diameter sterile puncher to take a bacterial cake from the edge of the colony of the activated pitaya canker pathogen Neoscytalidium dimidiatum, and inoculate it in the center of the drug-containing plate and the blank control plate. Each treatment is repeated 3 times. After inoculation, the plates are placed in a constant temperature incubator at 28℃. After 3 days, the colony diameter is measured by the cross method, and the inhibition rate is calculated as the treatment colony diameter / control colony diameter.

[0051] The test results showed that the inhibitory effect of CM or fungicide alone on the pathogen of pitaya canker was poor, while the combination of ZJLMBA1908 strain and fungicide could significantly improve the inhibition rate of pitaya canker pathogen, and the effect increased with the increase of fungicide concentration. When the volume ratio of fungicide: CM was 1:3, the synergistic antibacterial effect was the best. Among them, the enhanced effect of the combination of Spring Thunder·Quinoline Copper Suspension Concentrate and Spring Thunder·Royal Copper Wettable Powder with CM was the most significant. The inhibitory effect on the mycelial growth of pitaya canker pathogen was as follows: Figure 1 The statistical results are shown in Figure 2 As shown in the results, while reducing the dosage and concentration of CM and fungicides, the inhibitory effect on the mycelial growth of pitaya canker pathogens can be significantly improved.

[0052] The antibacterial statistical results after using 1.25 mg / L fungicide and CM are shown in Table 1. After 1.25 mg / L fungicide and CM were mixed in a volume ratio of 1:1, the inhibition rates increased by 24.81%, 24.98%, 30.92%, 32.42% and 59.56% respectively compared with the use of chemical fungicides alone; after 1.25 mg / L fungicide and CM were mixed in a volume ratio of 1:3, the inhibition rates increased by 31.84%, 32.19%, 36.72%, 40.1% and 69.24% respectively compared with the use of chemical fungicides alone.

[0053] Table 1 Antibacterial effect of 1.25 mg / L fungicide mixed with CM

[0054]

[0055] The antibacterial statistical results after using 2.5 mg / L fungicide and CM are shown in Table 2. After 2.5 mg / L fungicide and CM were mixed in a volume ratio of 1:1, the inhibition rates increased by 10.24%, 10.99%, 17.01%, 14.72% and 47.3% respectively compared with the use of chemical fungicides alone; after 2.5 mg / L fungicide and CM were mixed in a volume ratio of 1:3, the inhibition rates increased by 14.79%, 14.68%, 18.9%, 19.9% ​​and 57.42% respectively compared with the use of chemical fungicides alone.

[0056] Table 2 Antibacterial effect of 2.5 mg / L fungicide mixed with CM

[0057]

[0058] The antibacterial statistical results after using 5 mg / L fungicide and CM are shown in Table 3. After 5 mg / L fungicide and CM were mixed in a volume ratio of 1:1, the inhibition rates increased by 10.2%, 10.34%, 8.081%, 12.57% and 36.22% respectively compared with the use of chemical fungicides alone; after 5 mg / L fungicide and CM were mixed in a volume ratio of 1:3, the inhibition rates increased by 12.42%, 13.3%, 18.9%, 16.74% and 39.34% respectively compared with the use of chemical fungicides alone.

[0059] Table 3 Antibacterial effect of 5 mg / L fungicide mixed with CM

[0060]

[0061] In summary, the results show that the compounding of CM and chemical fungicides significantly increased the inhibitory effect of chemical fungicides on the pathogens of pitaya canker, especially when compounded with a lower concentration of fungicides (volume ratio of 1:3), the inhibitory rate increased significantly. Compounding biological fungicides with chemical fungicides can not only reduce the dosage of CM and chemical fungicides, but also further reduce the concentration of fungicides, thereby reducing the drug resistance caused by excessive use of chemical fungicides and the pollution to the environment, achieving a good inhibitory effect. For example, when the ZJLMBA1908 strain is compounded with Spring Thunder·King Copper WP, its inhibitory rate against the pathogens of pitaya canker can be increased from 20% to 94.17%.

[0062] Example 2 Inhibition rate of the composition on spore germination of pitaya canker pathogen

[0063] 1. Preparation of spore suspension

[0064] The activated hyphae at the edge of pitaya canker pathogen were picked and transferred to PDA plate. They were cultured at 25°C for 8 days in the dark. The spore-forming pathogen plate was added with 5 mL of sterile water and gently scraped with a sterile applicator. The spore suspension was filtered through sterile gauze and centrifuged for 5 min. The supernatant was removed and the concentration of the spore suspension was adjusted to 1 × 10 7 pcs / mL, for future use.

[0065] 2. Preparation of chemical agents and compositions

[0066] On the basis of Example 1, the concentration of the chemical fungicide was selected to be 1.25 mg / L to determine the inhibition rate of spore germination and the synergistic effect of the composition on the inhibition of spore germination. Prepare fungicidal solutions of different concentrations:

[0067] (1) As in Example 1, the prepared fungicide was added to sterile water at a ratio of 20 mL / 100 mL to prepare a solution with a fungicide concentration of 0.25 mg / L.

[0068] (2) CM (the concentration of sterile fermentation supernatant was 100 μL / mL) and chemical sterilization were added to sterile water at a volume ratio of 1:1 and a ratio of (10+10) mL / 100 mL to prepare a solution with a sterilization concentration of 0.125 mg / L.

[0069] (3) CM and chemical sterilization were added to sterile water at a volume ratio of 1:3 and a ratio of (15 + 5) mL / 100 mL to prepare a solution with a sterilization concentration of 0.0625 mg / L.

[0070] 3. Determination of spore germination rate

[0071] 30 μL of spore suspension was mixed with fermentation supernatant (CM), fungicide solution with a concentration of 0.25 mg / L, and combination solution of different volume ratios, respectively, and then dropped onto a clean and sterile concave slide, placed on a culture dish covered with wet filter paper, and cultured at 28°C for 24 h. Sterile water was used instead of the agent as a control. When the spore germination rate of the control reached more than 90%, the number of spore germinations was observed and recorded under an optical microscope, and the spore germination rate was calculated as the number of germinated spores / total number of spores; the corrected spore germination rate was then calculated as the spore germination rate in each treatment / spore germination rate in the control; finally, the calculation formula for the statistical spore germination rate was as follows:

[0072] Spore germination inhibition rate = (spore germination rate in control - corrected spore germination rate of each treatment) / spore germination rate in control.

[0073] The results are shown in Table 4, which shows that when the strain fermentation supernatant CM and 0.25 mg / L chemical fungicide are used alone, the inhibition rate of spore germination of pitaya canker pathogen is low, ranging from 40.57 to 61.05%; when the fungicide and CM are mixed in a ratio of 1:1, the inhibition rate of spore germination is in the range of 63.25 to 76.95%, which increases by 15.01%, 23.04%, 15.34%, 15.17% and 19.76%, respectively; when the fungicide and CM are mixed in a ratio of 1:3, the inhibition rate is in the range of 84.43% to 92.04%, which increases by 36.19%, 35.31%, 33.3%, 33.99% and 45.18%, respectively.

[0074] It shows that the combination of CM and fungicides significantly increases the inhibitory effect on the germination of pitaya canker pathogen spores, indicating that the combination of low concentrations of the five fungicides with CM can significantly inhibit the germination of pitaya canker pathogen spores, especially when combined with chlorpyrifos·quinoline copper suspension and chlorpyrifos·copper suspending agent, the effect is significant. Figure 3 As shown, there is a synergistic effect.

[0075] Table 4 Inhibitory effect of different agents on spore germination of pitaya canker

[0076]

[0077] Example 3: Prevention and treatment of pitaya canker by pharmaceutical composition

[0078] The pitaya stem segments with the same health status were randomly selected, rinsed with sterile water, disinfected with 75% ethanol for 5 minutes, and then rinsed with sterile water for use. The experimental groups were as follows: (1) 300 μL of pitaya canker pathogen spore suspension was first sprayed on the surface of the stem segment, and after the surface moisture of the stem segment dried, 300 μL of pharmaceutical composition 1 (final concentration of 1.25 mg / L of spring thunder·King copper suspension fungicide: CM=1:1) was sprayed; (2) 300 μL of pitaya canker pathogen spore suspension was first sprayed on the surface of the stem segment, and after the surface moisture of the stem segment dried, 300 μL of the above composition 2 (final concentration of 1.25 mg / L of spring thunder·King copper suspension fungicide: CM=1:3) was sprayed. After 2 hours, the leaves were covered with sterile plastic bags and the disease was continuously observed under the conditions of 28°C and 90% relative humidity. The above experiment was repeated every 3 days for a total of 3 times. Sterile water was used instead of the combined agent as a control. Five stem segments were treated in each group. After 15 days, the disease prevention efficiency was calculated based on the number of lesions formed on the leaves. The formula is as follows:

[0079]

[0080] Among them, N1 represents the number of lesions on the stem segment sprayed with a single spore suspension, and N2 represents the number of lesions on the leaves after being treated with different combinations of agents.

[0081] The control effect on pitaya canker is as follows Figure 4 As shown, compared with the control group, the prevention effect of the fungicide alone is not as good as CM, and the pharmaceutical composition using the strain and the fungicide has the best control effect on pitaya canker. According to statistics, the disease prevention efficiency of composition 1 (agent: CM = 1: 1) for pitaya canker is 51.6%, and the disease prevention efficiency of composition 2 (agent: CM = 1: 3) is 67.8%, indicating that the fermentation supernatant CM of Bacillus amyloliquefaciens ZJLMBA1908 strain and the chemical fungicide can not only reduce the dosage and fungicide concentration, but also greatly improve the control effect of pitaya canker. Based on the inhibitory effect of Example 1, it can be seen that the Bacillus amyloliquefaciens ZJLMBA1908 strain can also be used for the prevention and treatment of pitaya canker after being compounded with the fungicides benzoyl·pyraclostrobin suspension, pyraclostrobin·pyraclostrobin suspension, and pyrazole·krebs-butyl suspension, and has a certain control effect.

[0082] In summary, the present invention provides a synergistic antibacterial biological-chemical sterilization composition, containing Bacillus amyloliquefaciens fermentation broth and bactericide, through the combination of fungi and drugs, the fungicide and the microbial agent are compounded to have a synergistic antibacterial effect, which can significantly improve the inhibitory effect on the new dark columnar spore (Neoscytalidium dimidiatum), can inhibit the mycelial growth and spore germination of pitaya canker pathogens, and has a significant control effect on pitaya canker, and its effect is significantly better than that of using strains or bactericides alone; combining them and not only can reduce the dosage of bactericides and bactericides, but also can further reduce the concentration of bactericides, thereby reducing the drug resistance and environmental pollution caused by excessive use of chemical bactericides, and achieving a good inhibitory effect. Therefore, the drug-bacteria composition provided by the present invention can be better used in the prevention and treatment of pitaya canker during pitaya planting as an environmentally friendly, reduced, synergistic, and novel sterilization product. Among them, the copper preparations Chunlei·Quinoline Copper Suspension and Chunlei·Royal Copper Wettable Powder among the compound fungicides have the best effect. They not only broaden the application of copper preparations in the prevention and control of fungal diseases, but also improve the prevention and control efficiency of copper preparations on fungal diseases. It also provides more methods and products for the prevention and control of pitaya canker during pitaya cultivation, and has wide application value.

[0083] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A composition, characterized in that The invention comprises a fermentation liquid of Bacillus amyloliquefaciens ZJLMBA1908 strain and a chemical bactericide; the volume ratio of the fermentation liquid of ZJLMBA1908 strain to the chemical bactericide is (1-3):1; The ZJLMBA1908 strain was deposited in the General Microbiology Center of China Microbiological Culture Collection Administration on November 5, 2019, with the deposit number CGMCC No.18907; The chemical fungicide is selected from one or more of the group consisting of benzoyl-azoxystrobin suspension, pyraclostrobin-azoxystrobin suspension, pyraclostrobin suspension, pyraclostrobin-quinoline copper suspension, and pyraclostrobin-copper WP.

2. The composition according to claim 1, characterized in that The fermentation liquid is a sterile fermentation supernatant, and its concentration is not less than 100 μL / mL.

3. The composition according to claim 1, characterized in that The concentration of the chemical fungicide is ≥0.0625 mg / L.

4. Use of the composition according to claim 1 in preventing and treating pitaya canker.

5. Use of the composition according to claim 1 in inhibiting Neoscytalidium dimidiatum.

6. Use of the composition according to claim 1 in the preparation of antibacterial products.

7. An antibacterial product, characterized in that: Containing the composition according to claim 1.

8. A method for preventing and treating pitaya canker, characterized in that: The dragon fruit is treated with the composition according to claim 1 or the product according to claim 7.

9. Application of fermentation broth of Bacillus amyloliquefaciens ZJLMBA1908 as a synergist in improving the inhibitory effect of copper fungicides on fungi, characterized in that: The ZJLMBA1908 strain was deposited in the General Microbiology Center of the China Microbiological Culture Collection Administration on November 5, 2019, with the deposit number CGMCC No.18907; the copper preparation chemical fungicide is Chunlei·Quinoline Copper Suspension Concentrate or Chunlei·Royal Copper Wettable Powder.

10. The use according to claim 9, characterized in that: The fungus is Neopyrocoelenterospore; the inhibitory effect refers to the inhibition of hyphae growth and / or spore germination.

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