Biological feed and preparation method thereof and compound feed

Abstract

The invention discloses a biological feed and a preparation method thereof and a compound feed. The raw materials of the biological feed include: a fermentation agent and a fermentation substrate; the fermentation agent includes enterococcus faecalis and bacillus licheniformis; the preservation number of the enterococcus faecalis is CGMCC No. 25007; the preservation number of the bacillus licheniformis is CGMCC No. 31537; the viable count of the enterococcus faecalis is ≥ 8×10 9 CFU / g, the viable count of Bacillus licheniformis is ≥ 0.6×10 9 The biological feed of the present invention has a high content of antioxidant substances, thereby improving the antioxidant capacity of pork; the biological feed has a high content of acid-soluble protein, thereby improving the digestibility of the diet; the biological feed has a high content of volatile fatty acids, thereby further improving the muscle fat content.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a biological feed and a preparation method thereof and a compound feed. Background Art

[0002] Pork is an important part of the dietary structure of Chinese consumers. Large-scale genetic selection to increase lean meat rate and improve production efficiency has caused a significant decline in pork quality. At present, the main breeding breed in my country is the "Du Changda" foreign three-way pig, which is slightly inferior to domestic local breeds in meat color and flavor, and cannot meet people's pursuit of meat quality indicators such as pork sensory perception and pork flavor. With the improvement of living standards, consumers have higher and higher requirements for pork quality. Pork quality is a key factor affecting consumers' purchasing desire and consumption experience. For animal husbandry workers, how to produce safer, healthier and higher-quality pork through biotechnology and animal nutrition means, so that consumers can buy and eat with peace of mind, is of great significance. Therefore, how to improve pork quality and improve carcass traits has become a hot topic in the industry in recent years.

[0003] With the scale of livestock and poultry farming and the ban on antibiotics in feed, the animal epidemic prevention situation is becoming increasingly severe. Microbial fermented feed is widely used because it does not add antibiotics and other drugs and does not cause antibiotic drug residues. The group standard "General Technical Rules for Fermented Feed" (T / CSWSL002-2018) defines microbial fermented feed as: feed raw materials and microorganisms in accordance with relevant national regulations such as the "Catalogue of Feed Raw Materials (2013)" and the "Catalogue of Feed Additives (2013)", produced through fermentation engineering technology, and single feed and mixed feed containing microorganisms or their metabolites. During the feed fermentation process, through microbial metabolism, anti-nutritional factors such as non-starch polysaccharides in feed raw materials are decomposed into oligosaccharides, small peptides and other substances to reduce their anti-nutritional effects and improve feed utilization; at the same time, probiotics proliferate and produce a large number of functional metabolites such as organic acids, biological enzymes, vitamins, antimicrobial peptides and antioxidants, giving feed the probiotic function of replacing antibiotics and promoting growth. In pig farming, microbial fermented feed has a good performance. It can not only reduce feed costs, but also improve animal immunity and intestinal health, reduce the amount of drugs used in the production process, improve the quality of pork, and provide an effective way to produce green pork products.

[0004] In the existing technology, microbial fermented feed has done a lot to replace antibiotics to promote growth. The technical effect is mainly reflected in the reduction of breeding costs and the improvement of breeding environment. However, in production practice, it is still difficult to improve the meat quality of livestock and poultry and produce green and high-quality pork. Summary of the invention

[0005] In order to solve the above technical problems, the purpose of this application is to provide a biological feed and a preparation method and compound feed, so as to produce microbial fermented feed rich in specific antioxidants, volatile fatty acids and acid-soluble proteins, so as to carry out green quality livestock and poultry breeding.

[0006] The present invention provides a biological feed, the raw materials of which include: a fermentation agent and a fermentation substrate;

[0007] The fermentation bacteria include Enterococcus faecalis and Bacillus licheniformis;

[0008] The Enterococcus faecalis is named Enterococcus faecalis, and is deposited in the General Microbiology Center of China Microorganism Culture Collection Administration (CGMCC), with a deposit number of CGMCC NO.25007 and a deposit date of June 6, 2022;

[0009] The Bacillus licheniformis is named Bacillus licheniformis, and is deposited in the General Microbiology Center of China Microorganism Culture Collection Administration (CGMCC), with a deposit number of CGMCC No.31537 and a deposit date of August 5, 2024;

[0010] In the fermentation agent, the number of viable Enterococcus faecalis is ≥ 8×10 9 CFU / g, the viable count of Bacillus licheniformis is ≥ 0.6×10 9 CFU / g.

[0011] The fermentation agents in the biological feed of the present invention are Enterococcus faecalis with a preservation number of CGMCC No. 25007 and Bacillus licheniformis with a preservation number of CGMCC No. 31537. The fermentation agents obtained by compounding these two bacteria synergistically ferment the fermentation substrate to produce biological feed. On the one hand, the content of antioxidant substances in the biological feed can be increased, thereby improving the antioxidant capacity of pork; on the other hand, the content of acid-soluble protein in the feed can be increased, thereby improving the digestibility of the diet. Furthermore, the number of viable lactic acid bacteria in the fermentation agent of the present invention is ≥ 8×10 9 CFU / g, the number of viable Bacillus ≥ 0.6×10 9 CFU / g, which can increase the volatile fatty acid content in biological feed, thereby further increasing muscle fat content.

[0012] Preferably, the viable count of Enterococcus faecalis is 8×10 9 -12×10 9 CFU / g, and the viable count of Bacillus licheniformis was 0.6×10 9 -1.0×10 9 CFU / g.

[0013] The viable count of Enterococcus faecalis described in this protocol is 8×10 9 -12×10 9 CFU / g, and the viable count of Bacillus licheniformis was 0.6×10 9 -1.0×10 9 CFU / g, the viable counts of Enterococcus faecalis and Bacillus licheniformis are within the above ranges, which can further promote the production of beneficial fermentation products (antioxidants, acid-soluble proteins, volatile fatty acids), thereby achieving a full fermentation effect.

[0014] Preferably, the fermentation substrate comprises one or more of corn, soybean meal and wheat bran.

[0015] Preferably, the crude protein content of the corn is 7.0wt%-8.0wt%.

[0016] Preferably, the crude protein content of the soybean meal is 42.5wt%-46.5wt%.

[0017] Preferably, the crude protein content of the wheat bran is 15.0wt%-15.5wt%.

[0018] Preferably, based on weight proportions, corn comprises 24.5-34.5 parts, soybean meal comprises 25-35 parts, and wheat bran comprises 35-45 parts.

[0019] Preferably, the crude protein content of the fermentation substrate is ≥20.5wt%, and the net energy is ≥1800kcal / kg.

[0020] Preferably, the fermentation bacteria agent is 0.5-1.0 parts by weight.

[0021] In this solution, the fermentation agent is 0.5-1.0 parts by weight. The fermentation agent is within this range to ensure sufficient fermentation, thereby further promoting the production of beneficial fermentation products (antioxidants, acid-soluble proteins, volatile fatty acids).

[0022] The present invention provides a method for preparing the biological feed, comprising the following steps:

[0023] S1. Adding a fermentation agent and water to the fermentation substrate and mixing them evenly to obtain a fermentation mixture;

[0024] S2. Fermenting the fermentation mixture obtained in step S1 to obtain the biological feed.

[0025] Preferably, in step S1, the water is 30-45 parts by mass.

[0026] Preferably, in step S2, the fermentation temperature is 20°C-45°C.

[0027] The fermentation temperature of this scheme is 20°C-45°C. The fermentation temperature within this range can ensure sufficient fermentation, thereby further promoting the production of beneficial fermentation products (antioxidants, acid-soluble proteins, volatile fatty acids).

[0028] Preferably, in step S2, the fermentation time is 3-5 days.

[0029] The fermentation time of this scheme is 3-5 days. The fermentation time within this range can ensure sufficient fermentation, thereby further promoting the production of beneficial fermentation products (antioxidants, acid-soluble proteins, volatile fatty acids), while reducing the decomposition of fermentation products and improving the nutritional level.

[0030] Preferably, in step S2, the detection indicators of the biological feed obtained by fermentation are: pH ≤ 4.6, moisture content 38wt%-40wt%, total acid ≥ 25g / kg, lactic acid bacteria ≥ 1×10 8 , crude protein content ≥15wt%.

[0031] The invention also provides a compound feed, which comprises the biological feed, betaine and organic selenium.

[0032] The compound feed of the present invention comprises, on the one hand, the biological feed of the present invention, which contains a large amount of beneficial metabolites such as antioxidant enzymes, antioxidant substances, volatile fatty acids and acid-soluble proteins, thereby improving the digestibility of feed raw materials and improving pork quality; on the other hand, betaine and organic selenium are added, wherein betaine can reduce back fat thickness, increase eye muscle area, and increase lean meat rate, and can also significantly improve meat color, increase marbling score, and increase intramuscular fat, thereby improving meat quality and carcass quality; organic selenium has a high absorption rate, can be effectively deposited in tissues, and improves the body's antioxidant capacity. The biological feed, betaine and organic selenium in the compound feed work synergistically, which is conducive to increasing the antioxidant effect of pork, thereby maintaining cell membrane integrity, reducing drip loss, improving pork color, increasing intramuscular fat content, reducing shear force, improving pork tenderness, and thus improving pork quality.

[0033] Preferably, the betaine is extracted from natural beets and has an appearance of white small particles or powder with a content of ≥95wt%.

[0034] Preferably, calculated by mass fraction, the biological feed comprises 4%-20%, the betaine comprises 0.10%-0.15%, and the organic selenium comprises 0.01%-0.02%.

[0035] Preferably, the organic selenium includes at least one of yeast selenium and methionine selenium.

[0036] The yeast selenium in the feed formulated in this scheme has the positive effect of reducing the drip loss of pork and stabilizing the color of pork.

[0037] Preferably, the yeast selenium: total selenium (in terms of se): 2000-2400 mg / kg, moisture content (accounting for yeast selenium) ≤6.0wt%, crude ash content (accounting for yeast selenium) ≤8.0wt%, crude protein content (accounting for yeast selenium) ≥35wt%, and organic selenium content (accounting for total selenium) ≥98wt%. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 This is a comparison chart of the carcass colors of pigs fed with compound feed made from biological feeds in Example 1 and Comparative Example 1 after slaughter.

[0039] Figure 2 This is a comparison chart of the longest muscle color of pig backs 45 minutes after slaughter of pigs fed with compound feed made from biological feeds in Example 1 and Comparative Example 1.

[0040] Figure 3 This is a comparison chart of the longest muscle color of pig backs fed with compound feed made from biological feeds in Example 1 and Comparative Example 1 24 hours after slaughter.

[0041] Figure 4This is a comparison of the marbling patterns in the longissimus dorsi muscle of pigs fed with compound feed made from the biological feed of Example 1 and Comparative Example 1 24 hours after slaughter. DETAILED DESCRIPTION

[0042] In order to enable those skilled in the art to better understand the solutions of the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only embodiments of a part of the present invention, rather than all embodiments.

[0043] In all the following examples, Enterococcus faecalis was deposited in the General Microbiology Center of the China Microorganism Culture Collection Administration Committee (CGMCC) with a deposit number of CGMCC NO.25007 and a deposit date of June 6, 2022; Bacillus licheniformis was deposited in the General Microbiology Center of the China Microorganism Culture Collection Administration Committee (CGMCC) with a deposit number of CGMCC No.31537 and a deposit date of August 5, 2024.

[0044] Example 1

[0045] S1. Grind corn, wheat bran and soybean meal, and sieve them with a sieve aperture of 2.0 mm;

[0046] S2. According to the mass percentage, 29.5 parts of corn, 40 parts of wheat bran, 30 parts of soybean meal, and 0.5 parts of liquid fermentation agent (the number of viable bacteria of Enterococcus faecalis is 12×10 9 CFU / g, the number of viable bacteria of Bacillus licheniformis is 1.0×10 9 CFU / g), 38 parts of sterile water, mixing, and packaging to obtain a fermentation mixture;

[0047] S3. The fermentation mixture obtained in step S2 is kept warm and fermented at a temperature of 30-32° C. for 3 days to obtain biological feed.

[0048] Example 2

[0049] S1. Grind corn, wheat bran and soybean meal, and sieve them with a sieve aperture of 2.0 mm;

[0050] S2. According to the mass percentage, 24.5 parts of corn, 40 parts of wheat bran, 35 parts of soybean meal, and 0.5 parts of liquid fermentation agent (the number of viable Enterococcus faecalis is 8×10 9CFU / g, the number of viable bacteria of Bacillus licheniformis was 0.6×10 9 CFU / g), 40 parts of sterile water, mixing, and packaging to obtain a fermentation mixture;

[0051] S3. The fermentation mixture obtained in step S2 is kept warm and fermented at a temperature of 20-22° C. for 5 days to obtain biological feed.

[0052] Example 3

[0053] S1. Grind corn, wheat bran and soybean meal, and sieve them with a sieve aperture of 2.0 mm;

[0054] S2. According to the mass percentage, 34.5 parts of corn, 40 parts of wheat bran, 25 parts of soybean meal, and 1 part of liquid fermentation agent (the number of viable bacteria of Enterococcus faecalis is 10×10 9 CFU / g, the number of viable bacteria of Bacillus licheniformis was 0.8×10 9 CFU / g), 38 parts of sterile water, mixing, and packaging to obtain a fermentation mixture;

[0055] S3. The fermentation mixture obtained in step S2 is kept warm and fermented at a temperature of 40° C. to 45° C. for 4 days to obtain biological feed.

[0056] Comparative Example 1

[0057] S1. Grind corn, wheat bran and soybean meal, and sieve them with a sieve aperture of 2.0 mm;

[0058] S2. According to the mass percentage, 29.5 parts of corn, 40 parts of wheat bran, 30 parts of soybean meal, and 0.5 parts of liquid fermentation agent (lactic acid bacteria with a viable count of 12×10 9 CFU / g, Enterococcus faecalis (CICC 21869) purchased from Beijing Yuwei Technology Co., Ltd.; the viable count of Bacillus was 1.0×10 9 CFU / g, Bacillus licheniformis (CICC 100037) purchased from Wuhan Ruichen Standard Material Technology Co., Ltd.), 38 parts of sterile water, mixed and packaged to obtain a fermentation mixture;

[0059] S3. The fermentation mixture obtained in step S2 is kept warm and fermented at a temperature of 30° C. to 32° C. for 3 days to obtain biological feed.

[0060] The biological feeds prepared in Examples 1-3 and Comparative Example 1 were tested for antioxidant substances, antioxidant enzymes and nutrients.

[0061] Table 1 Content of antioxidant substances in biological feeds of Examples 1-3 and Comparative Example 1

[0062] project Comparative Example 1 Example 1 Example 2 Example 3 Glutathione, mg / g 11.52±2.32 18.97±2.63 17.74±2.58 16.34±1.89 Carnosine, μg / g 0 15.72±1.46 14.65±1.39 14.40±1.25 Folic acid, μg / g 3.75±0.87 7.67±1.04 7.44±0.95 7.21±0.89

[0063] As can be seen from Table 1, the contents of antioxidant substances (glutathione, carnosine, folic acid) in the biological feed prepared in Examples 1-3 are significantly higher than those in Comparative Example 1, which fully demonstrates that the use of the fermentation bacteria (Enterococcus faecalis and Bacillus licheniformis) of the present invention can significantly increase the content of antioxidant substances in the biological feed.

[0064] Table 2 Antioxidant enzyme content in biological feeds of Examples 1-3 and Comparative Example 1

[0065] project Comparative Example 1 Example 1 Example 2 Example 3 Total antioxidant enzymes, U / mg 0.73±0.04 1.26±0.21 1.21±0.18 1.08±0.09 Superoxide dismutase, U / mg 42.26±3.48 70.64±6.38 68.45±5.24 65.23±5.49 Glutathione peroxidase, U / mg 150.47±17.42 177.85±19.24 172.91±16.75 169.43±18.66

[0066] As can be seen from Table 2, the content of antioxidant enzymes in the biological feeds prepared in Examples 1-3 is significantly higher than that in Comparative Example 1, which fully demonstrates that the use of the fermentation bacteria (Enterococcus faecalis and Bacillus licheniformis) of the present invention can significantly increase the content of antioxidant enzymes in the biological feed.

[0067] Preparation of compound feed

[0068] Example 1 Compound feed prepared from biological feed

[0069] Phase 1 Complete Pig Feed:

[0070] Calculated by mass fraction, corn 70.70%, soybean meal (protein content 46wt%) 13.50%, wheat bran 8.00%, calcium hydrogen phosphate 0.70%, stone powder 1.00%, lysine 0.60%, methionine 0.04%, threonine 0.17%, tryptophan 0.04%, sodium chloride 0.43%, sodium bicarbonate 0.25%, composite multi-mineral 0.20% (providing copper 16.00mg / kg, iodine 1mg / kg, iron 80mg / kg, manganese 48.00mg / kg, selenium 0.05mg / kg, zinc 64mg / kg for complete feed), composite multi-vitamin 0.20% (providing vitamin A for complete feed) 1300IU / kg, vitamin D150IU / kg, vitamin E11IU / kg, vitamin K0.50mg / kg, biotin 0.05mg / kg, folic acid 0.30mg / kg, niacin 30.00mg / kg, pantothenic acid 7.00mg / kg, riboflavin 2.00mg / kg, thiamine 1.00mg / kg, vitamin B6 1.00mg / kg, vitamin B12 5.00μg / kg), 4% of the biological feed prepared in Example 1, 0.15% betaine, and 0.02% yeast selenium were mixed to obtain the stage 1 pig compound feed of Example 1.

[0071] Phase 2 Pig Compound Feed:

[0072] Calculated by mass fraction, corn 76.40%, soybean meal (protein content 46wt%) 8.00%, wheat bran 8.00%, calcium hydrogen phosphate 0.50%, stone powder 0.90%, lysine 0.70%, methionine 0.04%, threonine 0.18%, tryptophan 0.03%, sodium chloride 0.43%, sodium bicarbonate 0.25%, composite multi-mineral 0.20% (providing copper 16.00mg / kg, iodine 1mg / kg, iron 80mg / kg, manganese 48.00mg / kg, selenium 0.05mg / kg, zinc 64mg / kg for complete feed), composite multi-vitamin 0.20% (providing vitamin A 1300IU / kg, vitamin D150IU / kg, vitamin E11IU / kg, vitamin K 0.50mg / kg, biotin 0.05mg / kg, folic acid 0.30mg / kg, niacin 30.00mg / kg, pantothenic acid 7.00mg / kg, riboflavin 2.00mg / kg, thiamine 1.00mg / kg, vitamin B6 1.00mg / kg, vitamin B12 5.00μg / kg), 4% of the biological feed prepared in Example 1, 0.15% betaine, and 0.02% yeast selenium were mixed to obtain the stage 2 pig compound feed of Example 1.

[0073] Example 2 Compound feed prepared from biological feed

[0074] Phase 1 Pig Compound Feed:

[0075] The biological feed prepared in Example 2 was used to replace the biological feed prepared in Example 1, and the rest was the same as the stage 1 pig compound feed in Example 1.

[0076] Phase 2 Pig Compound Feed:

[0077] The biological feed prepared in Example 2 was used to replace the biological feed prepared in Example 1, and the rest was the same as the stage 2 pig compound feed in Example 1.

[0078] Example 3 Compound feed prepared from biological feed

[0079] Phase 1 Pig Compound Feed:

[0080] The biological feed prepared in Example 3 was used to replace the biological feed prepared in Example 1, and the rest was the same as the stage 1 pig compound feed in Example 1.

[0081] Phase 2 Pig Compound Feed:

[0082] The biological feed prepared in Example 3 was used to replace the biological feed prepared in Example 1, and the rest was the same as the stage 2 pig compound feed in Example 1.

[0083] Comparative Example 1 Compound feed prepared from biological feed

[0084] Phase 1 Pig Compound Feed:

[0085] The biological feed prepared in Comparative Example 1 was used to replace the biological feed prepared in Example 1, and the rest was the same as the stage 1 pig compound feed in Example 1.

[0086] Phase 2 Pig Compound Feed:

[0087] The biological feed prepared in Comparative Example 1 was used to replace the biological feed prepared in Example 1, and the rest was the same as the stage 2 pig compound feed in Example 1.

[0088] The nutrients in the compound feeds prepared from the biological feeds of Examples 1-3 and Comparative Example 1 were tested, and the results are shown in the following table:

[0089] Table 3 Nutrient content in compound feeds prepared from biological feeds of Examples 1-3 and Comparative Example 1

[0090]

[0091]

[0092] As can be seen from Table 3, the contents of short-chain volatile fatty acids (formic acid, acetic acid, propionic acid and butyric acid) and acid-soluble protein in the stage 1 and stage 2 compound feeds prepared by the biological feeds of Example 1, Example 2 and Example 3 are higher than those in Comparative Example 1. This fully demonstrates that the use of the biological feed of the present invention to prepare compound feed can increase the contents of volatile fatty acids (formic acid, acetic acid, propionic acid and butyric acid) and acid-soluble protein in the feed, thereby improving the digestibility of the diet and the muscle fat content.

[0093] Feeding experiment

[0094] A comparative test was conducted on the compound feed prepared from the biological feed of Example 1 of the present invention and Comparative Example 1. 1000 healthy D×L×Y growing pigs weighing about 60-70 kg were selected and randomly divided into two treatments, each with 500 pigs, and the compound feed prepared from the biological feed of Example 1 and Comparative Example 1 was used for feeding. The test period was 60 days.

[0095] The compound feed prepared by the biological feed of Example 2 and Example 3 of the present invention was tested, and 60 healthy D×L×Y growing pigs weighing about 60-70 kg were selected and randomly divided into 2 treatments, each with 30 pigs, and fed with 2 kinds of feeds respectively. The test period was 60 days.

[0096] The pigs fed with the compound feeds prepared from the biological feeds of Comparative Example 1 and Examples 1-3 were slaughtered and the longissimus dorsi muscle was left for 45 minutes and 24 hours, and then the meat color was scored. The results are shown in Table 4 below.

[0097] Table 4 Pork quality of Examples 1-3 and Comparative Example 1

[0098]

[0099]

[0100] The P value in Table 4 indicates the significance of the difference between Comparative Example 1 and Example 1. Different lowercase letters in the same row indicate significant differences (P < 0.05), and the same or no letters indicate no significant differences (P > 0.05); the reasonable range of L (brightness value) is 37-52; the chromaticity value is b / a (yellowness value / redness value), and the smaller the chromaticity value, the brighter the red of the flesh.

[0101] As shown in Table 4, the b value and chromaticity value of the longissimus dorsi muscle of the pig fed with the compound feed prepared by the biological feed in Example 1 were lower than those of the comparative example 1 45 minutes and 24 hours after slaughter; from the results of the colorimetric card scoring, the meat color scores of the longissimus dorsi muscle of the pig fed with the compound feed prepared by the biological feed in Example 1 were higher than those of the comparative example 1 45 minutes and 24 hours after slaughter; in terms of pH value, the values ​​of the longissimus dorsi muscle of the pig fed with the compound feed prepared by the biological feed in Example 1 were higher than those of the comparative example 1 45 minutes and 24 hours after slaughter. The above results fully illustrate that the pig fed with the compound feed prepared by the biological feed in Example 1 can increase the antioxidant effect of pork, thereby maintaining the integrity of the cell membrane, reducing drip loss, improving the color of pork, increasing the intramuscular fat content, reducing shear force, improving the tenderness of pork, and thus improving the quality of pork.

[0102] Figure 1 This is a comparison of the carcass colors of pigs fed with compound feed made from biological feeds in Example 1 and Comparative Example 1 after slaughter. Figure 1 It can be seen that the color of the pig carcasses after slaughter when the compound feed prepared from the biological feed in Example 1 is better than that in Comparative Example 1.

[0103] Figure 2 This is a comparison of the color of the longest muscle of the pig back 45 minutes after slaughter of pigs fed with compound feed made from biological feeds in Example 1 and Comparative Example 1. Figure 2 It can be seen that the color of the longest muscle of the pig back of the pigs fed with the compound feed prepared from the biological feed in Example 1 is better than that in Comparative Example 1 45 minutes after slaughter.

[0104] Figure 3 This is a comparison of the longest muscle color of pig backs fed with compound feed made from biological feeds in Example 1 and Comparative Example 1 24 hours after slaughter. Figure 3 It can be seen that the color of the longest muscle of the pig back of the pigs fed with the compound feed made from the biological feed in Example 1 is better than that in Comparative Example 1 24 hours after slaughter.

[0105] Figure 4 This is a comparison of the marbling patterns in the longissimus dorsi muscle of pigs fed with compound feed made from biological feeds in Example 1 and Comparative Example 1 24 hours after slaughter. Figure 4 It can be seen that the marbling in the longissimus dorsi muscle of pigs fed with the compound feed made from the biological feed in Example 1 24 hours after slaughter is better than that in Comparative Example 1.

[0106] The above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A biological feed, characterized in that: The raw materials of the biological feed include: fermentation bacteria and fermentation substrate; The fermentation bacteria include Enterococcus faecalis and Bacillus licheniformis; The Enterococcus faecalis is named Enterococcus faecalis, and is deposited in the General Microbiology Center of China Microorganism Culture Collection Administration (CGMCC), with a deposit number of CGMCC NO.25007 and a deposit date of June 6, 2022; The Bacillus licheniformis is named Bacillus licheniformis, and is deposited in the General Microbiology Center of China Microorganism Culture Collection Administration (CGMCC), with a deposit number of CGMCC No.31537 and a deposit date of August 5, 2024; In the fermentation agent, the number of viable Enterococcus faecalis is ≥ 8×10 9 CFU / g, the viable count of Bacillus licheniformis is ≥ 0.6×10 9 CFU / g.

2. The biological feed according to claim 1, characterized in that: The fermentation substrate includes one or more of corn, soybean meal and wheat bran.

3. The biological feed according to claim 2, characterized in that: Calculated by weight: 24.5-34.5 parts of corn, and / or 25-35 parts of soybean meal, and / or 35-40 parts of wheat bran.

4. The biological feed according to claim 3, characterized in that: Calculated by weight, the fermentation agent is 0.5-1.0 parts.

5. A method for preparing the biological feed according to any one of claims 1 to 4, characterized in that: The steps include: S1. Adding a fermentation agent and water to the fermentation substrate and mixing them evenly to obtain a fermentation mixture; S2. Fermenting the fermentation mixture obtained in step S1 to obtain the biological feed.

6. The method for preparing biological feed according to claim 5, characterized in that: In step S1, the water is 30-45 parts by weight.

7. The method for preparing the biological feed according to claim 5, characterized in that: In step S2, the fermentation temperature is 20°C-45°C.

8. The method for preparing the biological feed according to claim 5, characterized in that: In step S2, the fermentation time is 3-5 days.

9. A compound feed, characterized in that: The compound feed comprises the biological feed according to any one of claims 1 to 4, betaine and organic selenium.

10. The compound feed according to claim 9, characterized in that: Calculated by mass fraction, the biological feed comprises 4%-20%, the betaine comprises 0.10%-0.15%, and the organic selenium comprises 0.01%-0.02%.

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