Preparation method of novel feed additive shrimp shell enzyme powder

Abstract

The invention discloses a method for preparing a novel feed additive shrimp shell enzyme powder, and belongs to the technical field of feed additive preparation. The method comprises the following steps: collecting the discarded shrimp shells after shrimp processing from aquatic processing plants, taking out an appropriate amount each time according to the needs; selecting the raw materials to be fermented according to the ratio, crushing the raw materials to be fermented, sieving them, and then sterilizing them; after sterilization and detection, the raw materials to be fermented are cooled to a suitable inoculation temperature; after the temperature of the fermented raw materials reaches the inoculation temperature after sterilization, the probiotic strains are connected to the fermentation bottle of the fermentation raw materials, and placed in a shaking table for shaking fermentation. After the fermentation is completed, pour it into a sprayer for atomization spraying. The extracted biological preparation is rich in astaxanthin, probiotic enzymes and various trace elements. It is mixed with feed and fed to poultry, livestock and aquatic animals to promote metabolism, help intestinal absorption and digestion, improve animal immunity, reduce the occurrence of diseases, and improve feed utilization.

Description

Technical Field

[0001] The invention relates to the technical field of feed additive preparation, and in particular to a method for preparing a novel feed additive, shrimp shell enzyme powder. Background Art

[0002] Antibiotics have been widely used as veterinary drugs and feed additives in livestock and poultry production for many years due to their advantages such as broad antibacterial spectrum. However, the long-term and large-scale use of antibiotics has led to the imbalance of normal flora in the digestive tract of livestock and poultry, the decline of livestock and poultry immune function, drug residues in livestock and poultry products, and the development of drug resistance by pathogenic microorganisms, which has led to the reduction of the quality of livestock and poultry products and seriously restricted the productivity of livestock and poultry. Shrimp shells have many uses after fermentation, mainly including the production of degradable plastic wrap, organic fertilizers and the extraction of chitin. The high protein content in shrimp shells can meet the nutritional needs of such animals for rapid growth. Probiotics are bacteria that are beneficial to humans and animals. The research and wide application of probiotics as feed additives will bring a new situation for feed additives and bring huge economic benefits to livestock and poultry and aquaculture. Summary of the invention

[0003] The purpose of the present invention is to provide a method for preparing a novel feed additive shrimp shell enzyme powder, which is a biological preparation made by collecting shrimp shells discarded after processing from aquatic processing plants, fermenting them with microorganisms, and adding ingredients for aquatic animal growth and disease resistance. The biological preparation is rich in astaxanthin, probiotic enzymes and various trace elements. It is mixed with feed and fed to poultry, livestock and aquatic animals to promote metabolism, help intestinal absorption and digestion, improve animal immunity, reduce the occurrence of diseases, and improve feed utilization. It promotes calcium supplementation for animals and increases egg production for laying hens and ducks. The ammonia nitrogen content of feces discharged by animals after feeding is reduced, thereby improving the breeding environment and solving the problems in the prior art.

[0004] To achieve the above object, the present invention provides the following technical solution: a method for preparing a novel feed additive shrimp shell enzyme powder, comprising the following steps:

[0005] S1. Collect the discarded shrimp shells from the aquatic product processing plant after shrimp processing, remove impurities in order to keep them fresh, put them into the ice storage for quick freezing, and take out an appropriate amount each time according to the needs;

[0006] S2, selecting the raw materials to be fermented according to the ratio, crushing the raw materials to be fermented, sieving them, and then sterilizing them;

[0007] S3. After sterilization and testing, the raw materials to be fermented are cooled to the inoculation temperature;

[0008] S4, after the temperature of the sterilized fermentation raw material reaches the inoculation temperature, the probiotic strains are inoculated into the fermentation bottle of the fermentation raw material, and the fermentation is carried out in a shaker for shaking;

[0009] S5. After fermentation is completed, pour into a sprayer for atomization and spraying;

[0010] S6. The extracted biological agent is rich in astaxanthin, probiotic enzymes and various trace elements.

[0011] Preferably, the raw materials to be fermented include the following components by weight:

[0012] 3-8 parts of shrimp shells, 12-18 parts of molasses, 1-2 parts of salt, 0.1-0.2 parts of potassium dihydrogen phosphate, 0.1-0.3 parts of citric acid, 2-10 parts of liquid probiotic bacteria, 5-10 parts of Chinese medicine composition, 10-20 parts of pomace, and 70-85 parts of water.

[0013] The liquid bacteria include 1 to 5 parts of Candida utilis, 0.5 to 5 parts of Bacillus subtilis, 0.5 to 5.5 parts of lactic acid bacteria, and 0.5 to 5 parts of Saccharomyces cerevisiae;

[0014] Wherein, the lactic acid bacteria is selected from one or more of Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus casei and Lactobacillus salivarius.

[0015] Preferably, the fermentation equipment is a fermentation tank or a fermentation tank, the fermentation temperature is 37° C., and the fermentation time is 7 days (including 2 days of shaking fermentation in a shaking table).

[0016] Preferably, the volume mass fraction of the liquid bacteria added is 4-8%.

[0017] Preferably, in the step 2, the raw material to be fermented is crushed and passed through a 40-mesh sieve, sterilized at 121° C. for 15 min, and cooled to below 37° C.

[0018] Preferably, the traditional Chinese medicine composition includes 5-10 weight parts of Scutellaria barbata, 5-10 weight parts of Herba Psoraleae, 5-10 weight parts of Herba Portulacae, 5-10 weight parts of Herba Patriniae, 5-10 weight parts of Herba Prunellae, 5-10 weight parts of Herba Houttuyniae, 5-10 weight parts of Herba Scutellariae, 5-10 weight parts of Herba Forsythiae, and 5-10 weight parts of Licorice; 5-10 weight parts of Codonopsis pilosula, 5-10 weight parts of Herba Astragali, and 5-10 weight parts of Angelica sinensis.

[0019] Preferably, the preparation method of the Chinese medicine composition is specifically as follows: take the Chinese medicine component drugs, chop them and add them to an extraction tank, add 3000 parts by weight of water and soak them for 30 to 40 minutes, boil them for 1 hour, take out the first decoction for use; add 3000 parts by weight of water to the residue and boil them for 1 hour, take out the second decoction, combine the two decoctions and filter them, concentrate the filtrate under reduced pressure to 1000 parts by weight of the solution, let it stand for precipitation for 24 to 36 hours, absorb the supernatant, concentrate it to a relative density of 1.04-1.05, centrifuge it at a high speed of 14000-16000rps, take the supernatant and concentrate it to a relative density of 1.10-1.14.

[0020] Preferably, the raw material to be fermented further comprises montmorillonite and an inorganic metal complex, and the weight ratio of the montmorillonite to the inorganic metal complex is 1:2;

[0021] The inorganic metal composite is obtained by mixing strontium salt, gallium salt, lithium salt, zinc salt, chromium salt and copper salt, and the mass ratio of the strontium salt, gallium salt and lithium salt is 3:2:1.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] 1. The preparation method of shrimp shell enzyme powder proposed by the present invention uses fresh shrimp shells to extract biological preparations through probiotic fermentation, which is rich in astaxanthin, probiotic enzymes and various trace elements. It is mixed with feed and fed to poultry, livestock and aquatic animals to promote metabolism, help intestinal absorption and digestion, improve animal immunity, reduce the occurrence of diseases, and improve feed utilization. It promotes calcium supplementation for animals and increases egg production for laying hens and laying ducks. The ammonia nitrogen content of feces discharged by animals after feeding is reduced, thereby improving the breeding environment.

[0024] 2. The method for preparing shrimp shell enzyme powder proposed by the present invention improves the non-specific immunity of poultry, livestock and aquatic animals by adding Chinese herbal medicine additives to the enzyme powder, thereby enhancing the ability of poultry, livestock and aquatic animals to resist various diseases and their ability to adapt to the external environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 The present invention is a flow chart of the preparation method of the shrimp shell enzyme powder. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] See also Figure 1 , providing the following technical solutions:

[0028] A method for preparing a novel feed additive shrimp shell enzyme powder comprises the following steps:

[0029] S1. Collect the discarded shrimp shells from the aquatic product processing plant after shrimp processing, remove impurities in order to keep them fresh, put them into the ice storage for quick freezing, and take out an appropriate amount each time according to the needs;

[0030] S2, selecting the raw materials to be fermented according to the ratio, crushing the raw materials to be fermented, sieving them, and then sterilizing them;

[0031] S3. After sterilization and testing, the raw materials to be fermented are cooled to the inoculation temperature;

[0032] S4, after the temperature of the sterilized fermentation raw material reaches the inoculation temperature, the probiotic strains are inoculated into the fermentation bottle of the fermentation raw material, and the fermentation is carried out in a shaker for shaking;

[0033] S5. After fermentation is completed, pour into a sprayer for atomization and spraying;

[0034] S6. The extracted biological agent is rich in astaxanthin, probiotic enzymes and various trace elements.

[0035] The raw materials to be fermented include the following components by weight:

[0036] 3-8 parts of shrimp shells, 12-18 parts of molasses, 1-2 parts of salt, 0.1-0.2 parts of potassium dihydrogen phosphate, 0.1-0.3 parts of citric acid, 2-10 parts of liquid probiotic bacteria, 5-10 parts of Chinese medicine composition, 10-20 parts of pomace, and 70-85 parts of water.

[0037] The liquid bacteria include 1 to 5 parts of Candida utilis, 0.5 to 5 parts of Bacillus subtilis, 0.5 to 5.5 parts of lactic acid bacteria, and 0.5 to 5 parts of Saccharomyces cerevisiae;

[0038] Wherein, the lactic acid bacteria is selected from one or more of Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus casei and Lactobacillus salivarius.

[0039] The fermentation equipment is a fermentation tank or a fermentation tank, the fermentation temperature is 37°C, and the fermentation time is 7 days (including 2 days of shaking fermentation in a shaker). The volume mass fraction of the liquid strain added is 4-8%. In step 2, the raw material to be fermented is crushed through a 40-mesh sieve, sterilized at 121°C for 15 minutes, and cooled to below 37°C. The Chinese medicine composition includes 5-10 parts by weight of Scutellaria barbata, 5-10 parts by weight of Di Ding, 5-10 parts by weight of Portulaca oleracea, 5-10 parts by weight of Patrinia scabra, 5-10 parts by weight of Prunella vulgaris, 5-10 parts by weight of Houttuynia cordata, 5-10 parts by weight of Scutellaria baicalensis, 5-10 parts by weight of Forsythia suspensa, 5-10 parts by weight of Licorice; 5-10 parts by weight of Codonopsis pilosula, 5-10 parts by weight of Astragalus, and 5-10 parts by weight of Angelica sinensis.

[0040] The preparation method of the Chinese medicine composition is as follows: the Chinese medicine component is cut into pieces and added to an extraction tank, 3000 parts by weight of water is added and soaked for 30 to 40 minutes, and the first decoction is taken out for use after decocting for 1 hour; the medicinal residue is added with 3000 parts by weight of water and decocted for 1 hour, and the second decoction is taken out, the two decoctions are combined and filtered, the filtrate is concentrated to 1000 parts by weight of the solution, and the solution is allowed to stand for precipitation for 24 to 36 hours, the supernatant is absorbed, and the solution is concentrated to a relative density of 1.04-1.05, and the solution is centrifuged at a high speed of 14000-16000 rps, and the supernatant is concentrated to a relative density of 1.10-1.14; the raw materials to be fermented also include montmorillonite and an inorganic metal complex, and the weight ratio of the montmorillonite to the inorganic metal complex is 1:2; the inorganic metal complex is obtained by mixing strontium salt, gallium salt, lithium salt, zinc salt, chromium salt and copper salt, and the mass ratio of the strontium salt, gallium salt and lithium salt is 3:2:1.

[0041] Experiment of using shrimp shell enzyme powder in chicken farms:

[0042] Test period: March 10 to June 10, 2024

[0043] Test address: Chicken farm of Team 6, Huguang Farm, Zhanjiang City

[0044] Experimental method: Compare the experimental group and the control group.

[0045] Test process:

[0046] 40 three-yellow laying hens were randomly divided into a test group of 20 and a control group of 20. The average weight of the test group was The average weight of the control group was 1580g. The experiment started on March 10, 2024 and lasted for 3 months. A feeding test was conducted by adding 0.5% shrimp shell enzyme powder. The test results are recorded in the following table.

[0047]

[0048]

[0049] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A method for preparing a novel feed additive shrimp shell enzyme powder, comprising the following steps: S1. Collect the discarded shrimp shells from the aquatic product processing plant after shrimp processing, remove impurities in order to keep them fresh, put them into the ice storage for quick freezing, and take out an appropriate amount each time according to the needs; S2, selecting the raw materials to be fermented according to the ratio, crushing the raw materials to be fermented, sieving them, and then sterilizing them; S3. After sterilization and testing, the raw materials to be fermented are cooled to the inoculation temperature; S4, after the temperature of the sterilized fermentation raw material reaches the inoculation temperature, the probiotic strains are inoculated into the fermentation bottle of the fermentation raw material, and the fermentation is carried out in a shaker for shaking; S5. After fermentation is completed, pour into a sprayer for atomization and spraying; S6. The extracted biological agent is rich in astaxanthin, probiotic enzymes and various trace elements.

2. The method for preparing a novel feed additive shrimp shell enzyme powder according to claim 1, characterized in that: The raw materials to be fermented include the following components by weight: 3-8 parts of shrimp shells, 12-18 parts of molasses, 1-2 parts of salt, 0.1-0.2 parts of potassium dihydrogen phosphate, 0.1-0.3 parts of citric acid, 2-10 parts of liquid probiotics, 5-10 parts of Chinese medicine composition, 10-20 parts of pomaceous matter, 70-85 parts of water. The liquid bacteria include 1-5 parts of Candida utilis, 0.5-5 parts of Bacillus subtilis, 0.5-5.5 parts of lactic acid bacteria, and 0.5-5 parts of brewer's yeast; Wherein, the lactic acid bacteria is selected from one or more of Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus casei and Lactobacillus salivarius.

3. The method for preparing a novel feed additive shrimp shell enzyme powder according to claim 1, characterized in that: The fermentation equipment is a fermentation tank or a fermentation tank, the fermentation temperature is 35-37° C., and the fermentation time is 5-7 days.

4. The method for preparing a novel feed additive shrimp shell enzyme powder according to claim 1, characterized in that: The volume mass fraction of the liquid bacteria added is 4-8%.

5. The method for preparing a novel feed additive shrimp shell enzyme powder according to claim 1, characterized in that: In the step 2, the raw material to be fermented is crushed and passed through a 40-mesh sieve, sterilized at 120° C. to 121° C. for 15 minutes, and cooled to below 37° C.

6. The method for preparing a novel feed additive shrimp shell enzyme powder according to claim 2, characterized in that: The Chinese medicine composition comprises 5-10 parts by weight of Scutellaria barbata, 5-10 parts by weight of Herba Psoraleae, 5-10 parts by weight of Herba Portulacae Oleraceae, 5-10 parts by weight of Herba Patriniae, 5-10 parts by weight of Herba Prunellae, 5-10 parts by weight of Herba Houttuyniae, 5-10 parts by weight of Herba Scutellariae, 5-10 parts by weight of Fructus Forsythiae, 5-10 parts by weight of Radix Glycyrrhizae; 5-10 parts by weight of Codonopsis pilosulae, 5-10 parts by weight of Herba Astragali, and 5-10 parts by weight of Radix Angelicae Sinensis.

7. The method for preparing a novel feed additive shrimp shell enzyme powder according to claim 2, characterized in that: The preparation method of the Chinese medicine composition is specifically as follows: taking the Chinese medicine component drugs, cutting them into pieces and adding them into an extraction tank, adding 3000 parts by weight of water and soaking them for 30 to 40 minutes, decocting them for 1 hour, taking out the first decoction liquid for standby use; adding 3000 parts by weight of water to the residues and decocting them for 1 hour, taking out the second decoction liquid, combining the two decoction liquids and filtering them, concentrating the filtrate to 1000 parts by weight of the solution under reduced pressure, letting it stand for precipitation for 24 to 36 hours, absorbing the supernatant, concentrating it to a relative density of 1.04-1.05, centrifuging it at a high speed of 14000-16000 rps, and concentrating the supernatant to a relative density of 1.10-1.

14.

8. The method for preparing a novel feed additive shrimp shell enzyme powder according to claim 1, characterized in that: The raw material to be fermented further includes montmorillonite and an inorganic metal complex, and the weight ratio of the montmorillonite to the inorganic metal complex is 1:2; The inorganic metal composite is obtained by mixing strontium salt, gallium salt, lithium salt, zinc salt, chromium salt and copper salt, and the mass ratio of the strontium salt, gallium salt and lithium salt is 3:2:1.

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