Hermetia illucens breeding feed for increasing polypeptide yield and preparation method of hermetia illucens breeding feed

Abstract

The invention provides a hermetia illucens breeding feed for increasing the yield of polypeptides and a preparation method thereof. The feed is prepared from a polypeptide enhancer, a traditional Chinese medicine extract, a nutrition additive, a microbial regulator and trace elements. The polypeptide enhancer comprises a yeast extract, a seaweed extract and a vegetable protein hydrolysate in a specific proportion, and the yeast extract, the seaweed extract and the vegetable protein hydrolysate are elaborately prepared to maximize the polypeptide content. The traditional Chinese medicine extract is extracted from astragalus membranaceus, codonopsis pilosula, atractylodes macrocephala koidz, liquorice and other medicinal materials, and is rich in active ingredients. The nutrition additive and the microbial regulator are respectively selected from vitamins, probiotics and the like, so that the growth and health of the hermetia illucens are facilitated. In the preparation process, the raw materials are uniformly mixed, fermented, granulated and dried, and the final feed is obtained. According to the feed, the polypeptide yield of the hermetia illucens can be remarkably increased, and the bioavailability of the feed and the growth performance of the hermetia illucens are improved by optimizing the nutrition proportion and the fermentation technology.

Description

Technical Field

[0001] The invention relates to the technical field of feed, and in particular to a black soldier fly breeding feed for improving polypeptide yield and a preparation method thereof. Background Art

[0002] As people's demand for protein resources continues to grow, the cultivation of black soldier flies as a high-quality insect protein source has received more and more attention. Black soldier flies are rich in protein and a variety of nutrients, and the polypeptides in their bodies have a variety of important biological functions and application values. However, in the existing black soldier fly breeding technology, there are still some problems that need to be solved, resulting in the difficulty of black soldier fly polypeptide production to meet market demand.

[0003] 1. Unbalanced feed nutrition:

[0004] (1) Single protein source: The protein source of existing black soldier fly breeding feed is often relatively single, mostly relying on conventional plant protein or animal protein raw materials, and lacking a reasonable combination of multiple protein sources. This makes the amino acid composition in the feed not comprehensive enough to meet the needs of black soldier flies for growth and peptide synthesis for various amino acids, limiting the growth performance of black soldier flies and the yield and quality of peptides.

[0005] (2) Lack of functional ingredients: Traditional feeds usually contain few ingredients with special functions, such as substances that can promote peptide synthesis. The complex physiological metabolic process in black soldier flies requires specific nutritional factors to regulate and promote peptide synthesis, but existing feeds cannot provide effective support, resulting in low efficiency of peptide synthesis in black soldier flies.

[0006] 2. Imperfect extraction process:

[0007] (1) Simple raw material processing: In the process of extracting protein and other components from feed raw materials, the existing technology often adopts a relatively simple processing method. For the preparation of yeast extract, the existing technology only performs simple crushing and autolysis, and does not optimize the processing according to the characteristics of yeast cells, resulting in insufficient release of the effective ingredients in the yeast cells. The quality and activity of the extract are low, and it is unable to provide efficient nutritional support for black soldier flies.

[0008] (2) Imprecise enzymatic hydrolysis conditions: The selection of enzymes and the control of enzymatic hydrolysis conditions in the prior art are not precise enough, resulting in incomplete or excessive protein hydrolysis, and the resulting polypeptide molecular structure is unreasonable, which affects the efficiency of its absorption and utilization by black soldier flies, and further affects the polypeptide yield. Summary of the invention

[0009] In view of this, the present invention proposes a black soldier fly breeding feed for increasing polypeptide production and a preparation method thereof to solve the above problems.

[0010] The technical solution of the present invention is achieved as follows: A black soldier fly breeding feed for increasing polypeptide production: characterized in that it comprises the following raw materials in parts by weight: 60-80 parts of a polypeptide enhancer, 5-15 parts of a Chinese medicine extract, 2-10 parts of a nutritional additive, and 1-8 parts of a microbial regulator; the polypeptide enhancer is a yeast extract, a seaweed extract, and a plant protein hydrolyzate in a mass ratio of (3.5-8.5):(4.2-6.5):(8.8-10.2).

[0011] Furthermore, the yeast extract is prepared by washing and crushing brewer's yeast with an enzyme activity greater than 20,000 U / g, adding 10-15% salt to the yeast to induce yeast cell autolysis, adding 0.2-0.5% complex enzyme to the autolyzed yeast solution, wherein the complex enzyme is a glucan hydrolase and a nuclease in a mass ratio of 1:1-3, adjusting the material pH to 5.0-7.0, performing enzymolysis at 40-50°C for 18-28h, and crushing at 150-200MPa to obtain the yeast extract.

[0012] Furthermore, the seaweed extract is prepared by removing miscellaneous algae and foreign matter from the seaweed, washing it in a dilute acid solution, mincing and grinding it to make it into a uniform slurry, steaming the seaweed slurry at an absolute pressure of 2-4MPa and a temperature of 125-140°C for 1-3h to obtain a seaweed decomposition liquid, adding 2-5% of alginate lyase by volume of the decomposition liquid, adjusting the pH value to 12.0-14.0, performing enzymolysis at a temperature of 35-55°C for 4-8h, filtering, concentrating, and drying to obtain the seaweed extract.

[0013] Furthermore, the vegetable protein hydrolysate is prepared by mixing pumpkin seeds and hemp seeds in a mass ratio of (3-5):1 as raw materials, crushing the raw materials, passing through a 120-150 mesh sieve, performing high pressure treatment at a pressure of 100-300 MPa, and then performing ultrasonic treatment at a frequency of 5000-8000 Hz for 15-35 min, and continuously adding a composite enzyme, wherein the composite enzyme is bromelain and subtilisin in a ratio of (16-35):(8-10), and the addition amount is 1.5-3.5% of the raw material mass, and hydrolyzing for 4-6 h at a temperature of 50-70°C and a pH of 5.0-6.5, and the vegetable protein hydrolysate is enriched by a nanofiltration membrane with a molecular weight cutoff of less than 1000 Da.

[0014] Furthermore, the Chinese medicine extract includes a mixed extract of Astragalus, Codonopsis, Atractylodes and Licorice in a mass ratio of (8-12):(3-5):(10-15):(1-3).

[0015] Furthermore, the Chinese medicine extract is prepared by taking the above-mentioned astragalus, codonopsis, atractylodes and licorice, washing the medicinal materials, removing dirt and impurities, crushing them, passing through 10-20 mesh, and soaking the crushed medicinal materials in a 70-90% ethanol solution for 1-3 hours, wherein the mass ratio of the medicinal materials to the ethanol solution is 1:(8-12), adjusting the ultrasonic frequency to 20-50kHz, the power to 300-600W, and the temperature to 40-60°C for ultrasonic extraction for 30-60min, filtering, 3000-5000rpm, centrifuging for 10-15min, concentrating, and drying to obtain the Chinese medicine extract.

[0016] Furthermore, the nutritional additive is selected from any one or a combination of B vitamins, vitamin C, vitamin E and amino acids.

[0017] Furthermore, the microbial regulator comprises lactic acid bacteria, bifidobacterium and Bacillus subtilis in a mass ratio of (35-70):(25-52):(20-40), and the viable count of each of the lactic acid bacteria is higher than 1×10 12 cfu / ml.

[0018] Furthermore, a black soldier fly breeding feed for increasing polypeptide production also includes 0.5-3 parts of trace elements; the trace elements are selected from any one or a combination of copper, iron, zinc, manganese, magnesium, calcium, phosphorus, potassium, and selenium.

[0019] Furthermore, a method for preparing a black soldier fly breeding feed for increasing polypeptide production comprises the following steps:

[0020] S1. Put the prepared polypeptide enhancer, Chinese herbal extract, nutritional additives, microbial regulators or trace elements into a mixer and mix them at a speed of 500-800 r / min for 30-60 min;

[0021] S2, putting the mixed feed into a fermentation device for fermentation treatment, the fermentation temperature is 30-35°C, the humidity is 50-60%, the pH value is 6.0-7.5, and the fermentation time is 3-7 days to obtain a fermented mixture,

[0022] S3. The fermented mixed material is pelletized by a feed pelletizer, dried at 40-60° C. for 4-6 hours to make the moisture content of the feed 10-15%, and packaged after drying to obtain black soldier fly breeding feed.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] (1) Increase polypeptide production: The polypeptide enhancer is composed of yeast extract, seaweed extract and plant protein hydrolysate in a specific ratio, which provides black soldier flies with rich polypeptide precursors and nutritional factors that promote polypeptide synthesis. Yeast extract is rich in various amino acids and bioactive ingredients, which can promote the metabolism of black soldier flies; seaweed extract contains various trace elements and polysaccharides, which can enhance the immunity and stress resistance of black soldier flies, and provide necessary nutrients for polypeptide synthesis; plant protein hydrolysate is processed by a specific process, has high biological activity and easy absorption, and can effectively improve the synthesis efficiency of polypeptides in black soldier flies. Ingredients such as astragalus, codonopsis, atractylodes and licorice in traditional Chinese medicine extracts have the functions of regulating the physiological functions of black soldier flies, enhancing immunity and promoting growth, and indirectly increasing polypeptide production.

[0025] (2) Optimizing the preparation process: The mixing and stirring process ensures the uniform distribution of various raw materials, so that black soldier flies can obtain balanced nutrition when feeding. Fermentation treatment improves the nutritional structure of the feed, increases the number and activity of beneficial microorganisms, improves the digestibility and absorption rate of the feed, and is beneficial to the growth and peptide synthesis of black soldier flies.

[0026] (3) Enhance the health and adaptability of black soldier flies: The various ingredients in the feed work synergistically to enhance the immunity and stress resistance of black soldier flies, making them more resistant to diseases and adverse environmental influences during the breeding process, and improving their survival rate and growth performance; comprehensive nutrition and a good intestinal microecological environment help black soldier flies maintain a healthy state, improve their adaptability to different breeding environments, and provide a guarantee for large-scale breeding. DETAILED DESCRIPTION

[0027] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0028] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0029] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0030] Example 1

[0031] A black soldier fly breeding feed for increasing polypeptide production: characterized in that it comprises the following raw materials in parts by weight: 60 parts of polypeptide enhancer, 5 parts of Chinese herbal extract, 2 parts of nutritional additives, and 1 part of microbial regulator;

[0032] The polypeptide enhancer is yeast extract, seaweed extract and plant protein hydrolyzate in a mass ratio of 3.5:4.2:8.8;

[0033] The yeast extract is prepared by washing and crushing brewer's yeast with an enzyme activity greater than 20,000 U / g, adding 10% salt to the yeast to induce yeast cell autolysis, adding 0.2% complex enzyme to the autolyzed yeast solution, wherein the complex enzyme is a glucan hydrolase and a nuclease in a mass ratio of 1:1, adjusting the material pH to 5.0, performing enzymolysis at 40° C. for 18 hours, and crushing at 150 MPa to obtain the yeast extract;

[0034] The seaweed extract is prepared by removing miscellaneous algae and foreign matter from the seaweed, washing it in a dilute acid solution, grinding and pulverizing it to make it into a uniform slurry, steaming the seaweed slurry at an absolute pressure of 2MPa and a temperature of 125°C for 1h to obtain a seaweed decomposition liquid, adding 2-5% of alginate lyase by volume of the decomposition liquid, adjusting the pH value to 12.0, performing enzymolysis at a temperature of 35°C for 4h, filtering, concentrating, and drying to obtain the seaweed extract;

[0035] The vegetable protein hydrolysate is prepared by mixing pumpkin seeds and hemp seeds in a mass ratio of 3:1 as raw materials, crushing the raw materials, passing through a 120-mesh sieve, performing high-pressure treatment at a pressure of 100 MPa, and then performing ultrasonic treatment at a frequency of 5000 Hz for 15 minutes, adding a composite enzyme, wherein the composite enzyme is bromelain and subtilisin in a ratio of 16:8, and the addition amount is 1.5% of the raw material mass, and hydrolyzing for 4 hours at a temperature of 50° C. and a pH of 5.0, and enriching the vegetable protein hydrolysate with a nanofiltration membrane with a molecular weight cutoff of less than 1000 Da;

[0036] The Chinese medicine extract comprises a mixed extract of astragalus, codonopsis, atractylodes and licorice in a mass ratio of 8:3:10:1, the medicinal materials are washed, soil and impurities are removed, crushed, and passed through 10 meshes, and the crushed medicinal materials are placed in a 70% ethanol solution and soaked for 1 hour, the mass ratio of the medicinal materials to the ethanol solution is 1:8, and the ultrasonic frequency is adjusted to 20kHz, the power is 300W, and the temperature is 40°C for ultrasonic extraction for 30min, filtered, 3000rpm, centrifuged for 10min, concentrated, and dried to obtain the Chinese medicine extract;

[0037] The nutritional additives are selected from vitamin B group, vitamin C, and vitamin E in a mass ratio of 1:1:1;

[0038] The microbial regulator comprises lactic acid bacteria, bifidobacterium and Bacillus subtilis in a mass ratio of 35:25:20, and the number of viable bacteria is higher than 1×10 12 cfu / ml.

[0039] Example 2

[0040] A black soldier fly breeding feed for increasing polypeptide production: characterized in that it comprises the following raw materials in parts by weight: 80 parts of polypeptide enhancer, 15 parts of Chinese herbal extract, 10 parts of nutritional additives, and 8 parts of microbial regulator;

[0041] The polypeptide enhancer is yeast extract, seaweed extract and plant protein hydrolyzate in a mass ratio of 8.5:6.5:10.2;

[0042] The yeast extract is prepared by washing and crushing brewer's yeast with an enzyme activity greater than 20,000 U / g, adding 15% salt to the yeast to induce yeast cell autolysis, adding 0.5% complex enzyme to the autolyzed yeast solution, wherein the complex enzyme is glucan hydrolase and nuclease in a mass ratio of 1:3, adjusting the material pH to 7.0, performing enzymolysis at 50° C. for 28 hours, and crushing at 200 MPa to obtain the yeast extract;

[0043] The seaweed extract is prepared by removing miscellaneous algae and foreign matter from the seaweed, washing it in a dilute acid solution, grinding and pulverizing it to make it into a uniform slurry, steaming the seaweed slurry at an absolute pressure of 4MPa and a temperature of 140°C for 3h to obtain a seaweed decomposition liquid, adding 5% of the volume of the decomposition liquid alginate lyase, adjusting the pH value to 14.0, performing enzymolysis at a temperature of 55°C for 8h, filtering, concentrating, and drying to obtain the seaweed extract;

[0044] The vegetable protein hydrolysate is prepared by mixing pumpkin seeds and hemp seeds in a mass ratio of 5:1 as raw materials, crushing the raw materials, passing through a 150-mesh sieve, performing high-pressure treatment at a pressure of 300 MPa, and then performing ultrasonic treatment at a frequency of 8000 Hz for 35 minutes, adding a composite enzyme, wherein the composite enzyme is bromelain and subtilisin in a ratio of 35:10, and the addition amount is 3.5% of the raw material mass, and hydrolyzing for 6 hours at a temperature of 70° C. and a pH of 6.5, and enriching the vegetable protein hydrolysate with a nanofiltration membrane with a molecular weight cutoff of less than 1000 Da;

[0045] The Chinese medicine extract comprises a mixed extract of astragalus, codonopsis, atractylodes and licorice in a mass ratio of 12:5:15:3, the medicinal materials are washed, soil and impurities are removed, crushed, and passed through 20 meshes, and the crushed medicinal materials are placed in a 90% ethanol solution and soaked for 3 hours, the mass ratio of the medicinal materials to the ethanol solution is 1:12, and the ultrasonic frequency is adjusted to 50kHz, the power is 600W, and the temperature is 60°C for ultrasonic extraction for 60 minutes, filtered, 5000rpm, centrifuged for 15 minutes, concentrated, and dried to obtain the Chinese medicine extract;

[0046] The nutritional additive is selected from vitamin B group;

[0047] The microbial regulator comprises lactic acid bacteria, bifidobacterium and Bacillus subtilis in a mass ratio of 70:52:40, and the number of viable bacteria is higher than 1×10 12 cfu / ml;

[0048] The above-mentioned examples 1-2 adopt the following preparation method:

[0049] S1. Put the prepared polypeptide enhancer, Chinese herbal extract, nutritional additives and microbial regulator into a mixer and mix them at a speed of 700 r / min for 50 min;

[0050] S2, putting the mixed feed into a fermentation device for fermentation treatment, the fermentation temperature is 32°C, the humidity is 55%, the pH value is 6.5, the fermentation time is 5 days, and the fermentation mixture is obtained,

[0051] S3. The fermented mixed material is granulated by a feed granulator, and dried at 50° C. for 5 h to make the moisture content of the feed 12%. After drying, the feed is packaged to obtain black soldier fly breeding feed.

[0052] Example 3

[0053] A black soldier fly breeding feed for increasing polypeptide production: characterized in that it comprises the following raw materials in parts by weight: 70 parts of polypeptide enhancer, 10 parts of Chinese herbal extract, 8 parts of nutritional additives, 5 parts of microbial regulator, and 1.5 parts of trace elements;

[0054] The polypeptide enhancer is yeast extract, seaweed extract and plant protein hydrolyzate in a mass ratio of 5.5:5.0:9.2;

[0055] The yeast extract is prepared by washing and crushing brewer's yeast with an enzyme activity greater than 20,000 U / g, adding 12% salt to the yeast to induce yeast cell autolysis, adding 0.4% complex enzyme to the autolyzed yeast solution, wherein the complex enzyme is glucan hydrolase and nuclease in a mass ratio of 1:2, adjusting the material pH to 6.0, performing enzymolysis at 45° C. for 23 hours, and crushing at 180 MPa to obtain the yeast extract;

[0056] The seaweed extract is prepared by removing miscellaneous algae and foreign matter from the seaweed, washing it in a dilute acid solution, grinding and pulverizing it to make it into a uniform slurry, steaming the seaweed slurry at an absolute pressure of 3MPa and a temperature of 135°C for 2h to obtain a seaweed decomposition liquid, adding 3% of the volume of the decomposition liquid alginate lyase, adjusting the pH value to 13.0, performing enzymolysis at a temperature of 45°C for 6h, filtering, concentrating, and drying to obtain the seaweed extract;

[0057] The vegetable protein hydrolysate is prepared by mixing pumpkin seeds and hemp seeds in a mass ratio of 4:1 as raw materials, crushing the raw materials, passing through a 130-mesh sieve, performing high-pressure treatment at a pressure of 200 MPa, and then performing ultrasonic treatment at a frequency of 7000 Hz for 25 minutes, adding a composite enzyme, wherein the composite enzyme is bromelain and subtilisin in a ratio of 25:9, and the addition amount is 2.5% of the raw material mass, and hydrolyzing for 5 hours at a temperature of 60° C. and a pH of 6.0, and enriching the vegetable protein hydrolysate with a nanofiltration membrane with a molecular weight cutoff of less than 1000 Da;

[0058] The Chinese medicine extract comprises a mixed extract of astragalus, codonopsis, atractylodes and licorice in a mass ratio of 10:4:12:2, the medicinal materials are washed, soil and impurities are removed, crushed, and passed through 15 meshes, and the crushed medicinal materials are placed in an 80% ethanol solution and soaked for 2 hours, the mass ratio of the medicinal materials to the ethanol solution is 1:10, and the ultrasonic frequency is adjusted to 45kHz, the power is 500W, and the temperature is 50°C for ultrasonic extraction for 50min, filtered, 4000rpm, centrifuged for 12min, concentrated, and dried to obtain the Chinese medicine extract;

[0059] The nutritional additives are selected from B vitamins, vitamin C, vitamin E and amino acids in a mass ratio of 1:1:1:1;

[0060] The microbial regulator comprises lactic acid bacteria, bifidobacterium and Bacillus subtilis in a mass ratio of 60:42:30, and the number of viable bacteria is higher than 1×10 12 cfu / ml;

[0061] The trace elements are selected from copper, iron and zinc elements in a mass ratio of 1:2:1;

[0062] The above-mentioned Example 3 adopts the following preparation method:

[0063] S1. Put the prepared polypeptide enhancer, Chinese herbal extract, nutritional additives, microbial regulator and trace elements into a mixer and mix them at a speed of 700 r / min for 50 min;

[0064] S2, putting the mixed feed into a fermentation device for fermentation treatment, the fermentation temperature is 32°C, the humidity is 55%, the pH value is 6.5, the fermentation time is 5 days, and the fermentation mixture is obtained.

[0065] S3. The fermented mixed material is granulated by a feed granulator, and dried at 50° C. for 5 h to make the moisture content of the feed 12%. After drying, the feed is packaged to obtain black soldier fly breeding feed.

[0066] Example 4

[0067] A black soldier fly breeding feed for increasing polypeptide production: characterized in that it comprises the following raw materials in parts by weight: 70 parts of polypeptide enhancer, 10 parts of Chinese herbal extract, 8 parts of nutritional additives, 5 parts of microbial regulator, and 1.5 parts of trace elements;

[0068] The polypeptide enhancer is yeast extract, seaweed extract and plant protein hydrolyzate in a mass ratio of 5.5:5.0:9.2;

[0069] The yeast extract is prepared by washing and crushing brewer's yeast with an enzyme activity greater than 20,000 U / g, adding 12% salt to the yeast to induce yeast cell autolysis, adding 0.4% complex enzyme to the autolyzed yeast solution, wherein the complex enzyme is glucan hydrolase and nuclease in a mass ratio of 1:2, adjusting the material pH to 6.0, performing enzymolysis at 45° C. for 23 hours, and crushing at 180 MPa to obtain the yeast extract;

[0070] The seaweed extract is prepared by removing miscellaneous algae and foreign matter from the seaweed, washing it in a dilute acid solution, grinding and pulverizing it to make it into a uniform slurry, steaming the seaweed slurry at an absolute pressure of 3MPa and a temperature of 135°C for 2h to obtain a seaweed decomposition liquid, adding 3% of the volume of the decomposition liquid alginate lyase, adjusting the pH value to 13.0, performing enzymolysis at a temperature of 45°C for 6h, filtering, concentrating, and drying to obtain the seaweed extract;

[0071] The vegetable protein hydrolysate is prepared by mixing pumpkin seeds and hemp seeds in a mass ratio of 4:1 as raw materials, crushing the raw materials, passing through a 130-mesh sieve, performing high-pressure treatment at a pressure of 200 MPa, and then performing ultrasonic treatment at a frequency of 7000 Hz for 25 minutes, adding a composite enzyme, wherein the composite enzyme is bromelain and subtilisin in a ratio of 25:9, and the addition amount is 2.5% of the raw material mass, and hydrolyzing for 5 hours at a temperature of 60° C. and a pH of 6.0, and enriching the vegetable protein hydrolysate with a nanofiltration membrane with a molecular weight cutoff of less than 1000 Da;

[0072] The Chinese medicine extract comprises a mixed extract of astragalus, codonopsis, atractylodes and licorice in a mass ratio of 10:4:12:2, the medicinal materials are washed, soil and impurities are removed, crushed, and passed through 15 meshes, and the crushed medicinal materials are placed in an 80% ethanol solution and soaked for 2 hours, the mass ratio of the medicinal materials to the ethanol solution is 1:10, and the ultrasonic frequency is adjusted to 45kHz, the power is 500W, and the temperature is 50°C for ultrasonic extraction for 50min, filtered, 4000rpm, centrifuged for 12min, concentrated, and dried to obtain the Chinese medicine extract;

[0073] The nutritional additives are selected from B vitamins, vitamin C, vitamin E and amino acids in a mass ratio of 1:1:1:1;

[0074] The microbial regulator comprises lactic acid bacteria, bifidobacterium and Bacillus subtilis in a mass ratio of 60:42:30, and the number of viable bacteria is higher than 1×10 12 cfu / ml;

[0075] The trace elements are selected from copper, iron and zinc elements in a mass ratio of 1:2:1;

[0076] The above-mentioned Example 4 adopts the following preparation method:

[0077] S1. Put the prepared polypeptide enhancer, Chinese herbal extract, nutritional additives, microbial regulator and trace elements into a mixer and mix them at a speed of 500 r / min for 30 min;

[0078] S2, putting the mixed feed into a fermentation device for fermentation treatment, the fermentation temperature is 30°C, the humidity is 50%, the pH value is 6.0, and the fermentation time is 3 days to obtain a fermentation mixture,

[0079] S3. The fermented mixed material is pelletized by a feed pelletizer, dried at 40° C. for 4 h to make the moisture content of the feed 10%, and packaged after drying to obtain black soldier fly breeding feed.

[0080] Example 5

[0081] A black soldier fly breeding feed for increasing polypeptide production: characterized in that it comprises the following raw materials in parts by weight: 70 parts of polypeptide enhancer, 10 parts of Chinese herbal extract, 8 parts of nutritional additives, 5 parts of microbial regulator, and 1.5 parts of trace elements;

[0082] The polypeptide enhancer is yeast extract, seaweed extract and plant protein hydrolyzate in a mass ratio of 5.5:5.0:9.2;

[0083] The yeast extract is prepared by washing and crushing brewer's yeast with an enzyme activity greater than 20,000 U / g, adding 12% salt to the yeast to induce yeast cell autolysis, adding 0.4% complex enzyme to the autolyzed yeast solution, wherein the complex enzyme is glucan hydrolase and nuclease in a mass ratio of 1:2, adjusting the material pH to 6.0, performing enzymolysis at 45° C. for 23 hours, and crushing at 180 MPa to obtain the yeast extract;

[0084] The seaweed extract is prepared by removing miscellaneous algae and foreign matter from the seaweed, washing it in a dilute acid solution, grinding and pulverizing it to make it into a uniform slurry, steaming the seaweed slurry at an absolute pressure of 3MPa and a temperature of 135°C for 2h to obtain a seaweed decomposition liquid, adding 3% of the volume of the decomposition liquid alginate lyase, adjusting the pH value to 13.0, performing enzymolysis at a temperature of 45°C for 6h, filtering, concentrating, and drying to obtain the seaweed extract;

[0085] The vegetable protein hydrolysate is prepared by mixing pumpkin seeds and hemp seeds in a mass ratio of 4:1 as raw materials, crushing the raw materials, passing through a 130-mesh sieve, performing high-pressure treatment at a pressure of 200 MPa, and then performing ultrasonic treatment at a frequency of 7000 Hz for 25 minutes, adding a composite enzyme, wherein the composite enzyme is bromelain and subtilisin in a ratio of 25:9, and the addition amount is 2.5% of the raw material mass, and hydrolyzing for 5 hours at a temperature of 60° C. and a pH of 6.0, and enriching the vegetable protein hydrolysate with a nanofiltration membrane with a molecular weight cutoff of less than 1000 Da;

[0086] The Chinese medicine extract comprises a mixed extract of astragalus, codonopsis, atractylodes and licorice in a mass ratio of 10:4:12:2, the medicinal materials are washed, soil and impurities are removed, crushed, and passed through 15 meshes, and the crushed medicinal materials are placed in an 80% ethanol solution and soaked for 2 hours, the mass ratio of the medicinal materials to the ethanol solution is 1:10, and the ultrasonic frequency is adjusted to 45kHz, the power is 500W, and the temperature is 50°C for ultrasonic extraction for 50min, filtered, 4000rpm, centrifuged for 12min, concentrated, and dried to obtain the Chinese medicine extract;

[0087] The nutritional additives are selected from B vitamins, vitamin C, vitamin E and amino acids in a mass ratio of 1:1:1:1;

[0088] The microbial regulator comprises lactic acid bacteria, bifidobacterium and Bacillus subtilis in a mass ratio of 60:42:30, and the number of viable bacteria is higher than 1×10 12 cfu / ml;

[0089] The trace elements are selected from copper, iron and zinc elements in a mass ratio of 1:2:1;

[0090] The above-mentioned Example 5 adopts the following preparation method:

[0091] S1. Put the prepared polypeptide enhancer, Chinese herbal extract, nutritional additives, microbial regulator and trace elements into a mixer and mix them at a speed of 800r / min for 30-60min;

[0092] S2, putting the mixed feed into a fermentation device for fermentation treatment, the fermentation temperature is 35°C, the humidity is 60%, the pH value is 7.5, the fermentation time is 7 days, and the fermentation mixture is obtained,

[0093] S3. The fermented mixed material is pelletized by a feed pelletizer, and dried at 60° C. for 6 h to make the moisture content of the feed 15%. After drying, the feed is packaged to obtain black soldier fly breeding feed.

[0094] Comparative Example 1

[0095] The difference between this comparative example and Example 3 is that the black soldier fly breeding feed comprises the following raw materials in parts by weight: 50 parts of polypeptide enhancer, 18 parts of Chinese herbal extract, 20 parts of nutritional additives, and 10 parts of microbial regulator.

[0096] Comparative Example 2

[0097] The difference between this comparative example and Example 3 is that no polypeptide enhancer is added to the black soldier fly breeding feed.

[0098] Comparative Example 3

[0099] The difference between this comparative example and Example 3 is that no Chinese medicine extract is added to the black soldier fly breeding feed.

[0100] Test Example 1-Nutrient Analysis

[0101] The protein content and polypeptide content of the feeds prepared in the above Examples 1-5 and Comparative Examples 1-3 were measured:

[0102] Protein content (%) Peptide content (mg / g) Example 1 48.5 161 Example 2 47.8 160 Example 3 50.1 168 Example 4 49.5 162 Example 5 49.2 165 Comparative Example 1 42.5 110 Comparative Example 2 44.1 108 Comparative Example 3 41.2 105

[0103] By comparing the protein content and polypeptide content data of the embodiments and comparative examples, it can be clearly seen that the feed formula of the present invention has significant advantages in increasing protein content and promoting polypeptide synthesis, providing strong support for improving the breeding efficiency and polypeptide yield of black soldier flies.

[0104] Test Example 2-Evaluation of Black Soldier Fly Growth Performance

[0105] (1) Growth rate determination: Under the same breeding environment, black soldier flies were cultured using the feeds prepared in Examples 1-5 and Comparative Example 1. The growth time of the black soldier flies from the larvae to the pupae was recorded. The changes in growth rate were intuitively reflected by regularly measuring the body length and weight of the black soldier flies.

[0106] (2) Survival rate statistics: Observe and record the survival rate of black soldier flies after they become adults.

[0107]

[0108]

[0109] In the black soldier fly breeding feed of the present invention, the embodiment groups all showed a higher body length growth rate, among which the feed of Example 3 made the body length growth rate of black soldier flies the fastest (2.7 mm / day), and the average weight growth was the same. In terms of survival rate, the feeds of Examples 1-5 all showed a higher survival rate, among which the feed of Example 3 made the survival rate of black soldier flies the highest (95.5%); overall, the feeds of Examples 1-5 were superior to the feeds of Comparative Examples 1-3 in promoting the growth rate of black soldier flies and improving the survival rate; among Examples 1-5, the feed of Example 3 performed best in promoting the growth rate and improving the survival rate, indicating that the formula and proportion of the present invention have better promotion of the growth of black soldier flies and improvement of the survival rate.

[0110] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A black soldier fly breeding feed for increasing polypeptide production, characterized in that: The invention comprises the following raw materials in parts by weight: 60-80 parts of polypeptide enhancer, 5-15 parts of traditional Chinese medicine extract, 2-10 parts of nutritional additives and 1-8 parts of microbial regulator; the polypeptide enhancer is yeast extract, seaweed extract and plant protein hydrolyzate in a mass ratio of (3.5-8.5):(4.2-6.5):(8.8-10.2).

2. The black soldier fly breeding feed for increasing polypeptide production according to claim 1, characterized in that: The yeast extract is prepared by washing and crushing brewer's yeast with an enzyme activity greater than 20,000 U / g, adding 10-15% salt to the yeast to induce yeast cell autolysis, adding 0.2-0.5% complex enzyme to the autolyzed yeast solution, wherein the complex enzyme is glucan hydrolase and nuclease in a mass ratio of 1:1-3, adjusting the pH of the material to 5.0-7.0, performing enzymolysis at 40-50°C for 18-28 hours, and crushing at 150-200 MPa to obtain the yeast extract.

3. The black soldier fly breeding feed for increasing polypeptide production according to claim 1, characterized in that: The seaweed extract is prepared by removing miscellaneous algae and foreign matters from the seaweed, washing it in a dilute acid solution, grinding and pulverizing it to make it into a uniform slurry, steaming the seaweed slurry at an absolute pressure of 2-4MPa and a temperature of 125-140°C for 1-3h to obtain a seaweed decomposition liquid, adding 2-5% of alginate lyase by volume of the decomposition liquid, adjusting the pH value to 12.0-14.0, performing enzymolysis at a temperature of 35-55°C for 4-8h, filtering, concentrating, and drying to obtain the seaweed extract.

4. The black soldier fly breeding feed for increasing polypeptide production according to claim 1, characterized in that: The vegetable protein hydrolysate is prepared by mixing pumpkin seeds and hemp seeds in a mass ratio of (3-5):1 as raw materials, crushing the raw materials, passing through a 120-150 mesh sieve, performing high-pressure treatment at a pressure of 100-300 MPa, and then performing ultrasonic treatment at a frequency of 5000-8000 Hz for 15-35 minutes, adding a composite enzyme, wherein the composite enzyme is bromelain and subtilisin in a ratio of (16-35):(8-10), and the added amount is 1.5-3.5% of the mass of the raw materials, and hydrolyzing for 4-6 hours at a temperature of 50-70°C and a pH of 5.0-6.5, and enriching the vegetable protein hydrolysate with a nanofiltration membrane with a molecular weight cutoff of less than 1000 Da.

5. The black soldier fly breeding feed for increasing polypeptide production according to claim 1, characterized in that: The Chinese medicine extract comprises a mixed extract of astragalus, codonopsis, atractylodes and liquorice in a mass ratio of (8-12):(3-5):(10-15):(1-3).

6. A black soldier fly breeding feed for increasing polypeptide production as claimed in claim 5, characterized in that: The Chinese medicine extract is prepared by washing the astragalus, codonopsis, atractylodes and liquorice, removing dirt and impurities, crushing, passing through 10-20 meshes, and soaking the crushed medicinal materials in an ethanol solution with a concentration of 70-90% for 1-3 hours, wherein the mass ratio of the medicinal materials to the ethanol solution is 1:(8-12), adjusting the ultrasonic frequency to 20-50kHz, the power to 300-600W, and the temperature to 40-60°C for ultrasonic extraction for 30-60min, filtering, centrifuging at 3000-5000rpm for 10-15min, concentrating, and drying to obtain the Chinese medicine extract.

7. The black soldier fly breeding feed for increasing polypeptide production according to claim 1, characterized in that: The nutritional additive is selected from any one or a combination of B vitamins, vitamin C, vitamin E and amino acids.

8. The black soldier fly breeding feed for increasing polypeptide production according to claim 1, characterized in that: The microbial regulator comprises lactic acid bacteria, bifidobacteria and Bacillus subtilis in a mass ratio of (35-70):(25-52):(20-40), and the number of viable bacteria is higher than 1×10 12 cfu / ml.

9. The black soldier fly breeding feed for increasing polypeptide production according to claim 1, characterized in that: It also includes 0.5-3 parts of trace elements; the trace elements are selected from any one or a combination of copper, iron, zinc, manganese, magnesium, calcium, phosphorus, potassium and selenium.

10. The method for preparing a black soldier fly breeding feed for increasing polypeptide production according to claim 1, characterized in that: The following steps are involved: S1. Put the prepared polypeptide enhancer, Chinese herbal extract, nutritional additives and microbial regulator into a mixer and mix them at a speed of 500-800r / min for 30-60min; S2, putting the mixed feed into a fermentation device for fermentation treatment, the fermentation temperature is 30-35°C, the humidity is 50-60%, the pH value is 6.0-7.5, and the fermentation time is 3-7 days to obtain a fermented mixture, S3. The fermented mixed material is pelletized by a feed pelletizer, dried at 40-60° C. for 4-6 hours to make the moisture content of the feed 10-15%, and packaged after drying to obtain black soldier fly breeding feed.

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