Composition with immunity enhancing effect and preparation method thereof

Abstract

This patent application discloses a composition with an immune-enhancing effect and a preparation method thereof. The immune-enhancing composition of the present invention comprises a ginseng fermentation product produced by probiotic fermentation, wherein rare ginsenosides Rk1, Rg5, Rk3, Rh4 and Rg3 with immune-enhancing effects account for more than 85% by weight of the total ginsenosides. Experimental studies have shown that the immune-enhancing composition of the present invention has a significant immune-enhancing effect.

Description

Technical Field

[0001] The present invention belongs to the field of medical biotechnology or food biotechnology, and specifically relates to a composition rich in rare ginsenosides and having the effect of enhancing immunity, and a preparation method and application thereof. Background Art

[0002] Ginseng is a perennial herbaceous plant of the genus Panax in the Araliaceae family (Panax ginseng CA Mayer). It has multiple functions, including tonifying the spleen and lungs, tonifying qi, calming, anti-anxiety, anti-depression, improving eyesight and improving intelligence, and is known as the "King of Chinese Medicine". It is listed as a top-grade product in the "Shennong's Herbal Classic", which clearly records that it can tonify the five internal organs, calm the spirit, stabilize the soul, stop panic, remove evil spirits, improve eyesight and make people happy and intelligent. In 2012, the Ministry of Health approved artificially cultivated ginseng of 5 years or less as a new resource food. At present, the form of ginseng products is relatively single, and the application range in the food field is limited. In view of this, the development of food, health care products and other products with ginseng as the main raw material has broad market prospects.

[0003] Ginseng contains a variety of active ingredients, such as ginsenosides and ginseng polysaccharides. Ginsenosides can regulate the body's immune cells, including T lymphocytes, B lymphocytes and natural killer cells. It can promote the proliferation and activation of immune cells, enhance the phagocytic ability and killing activity of immune cells, thereby improving the body's immune defense function. At the same time, ginseng polysaccharides can stimulate the activity of antigen-presenting cells such as macrophages and dendritic cells, and promote the body's recognition and elimination of pathogens. In addition, ginseng can also regulate the secretion of immune-related cytokines, maintain immune balance, and enhance the body's resistance to various diseases. In terms of anti-fatigue, ginseng can improve the body's energy metabolism level. It can promote the synthesis of adenosine triphosphate (ATP) and increase energy reserves in cells. The active ingredients in ginseng can also regulate the function of the nervous system and reduce fatigue.

[0004] Ginsenosides are one of the main active ingredients of ginseng. The main ginsenosides Rb1, Rb2, Rc, Rd, Rf and Rg1 account for more than 90% of the total ginsenosides, have multiple pharmacological effects, and account for about 4% of the total mass of ginseng. Rare saponins with extremely low content in Chinese medicinal materials such as ginseng have better pharmacological activity, but the content of rare ginsenosides in ginseng is usually very low. Generally, the content of rare ginsenosides such as Rg3, Rh2, Rk2, Rh3, aPPD, etc. in fresh ginseng is extremely small, which may only account for one hundred thousandth of the total ginsenosides of ginseng or even less. Therefore, how to convert the total saponins in ginseng into rare saponins is of great significance for the important pharmacological activity of ginsenosides. It can not only improve the efficacy of ginseng food, but also has important guiding significance for the production of rare saponins.

[0005] In the research on the production technology of rare ginsenosides, the biotransformation method of ginseng fermented by microorganisms is considered to be one of the safest and most efficient transformation methods for ginsenosides in vitro. In fact, technicians have used one microorganism or a combination of multiple microorganisms to ferment ginseng fermentation matrix to produce a variety of rare ginsenosides.

[0006] However, the technical problem of these existing technologies is that the microbial fermentation produces a combination of multiple rare ginsenosides, which may contain up to dozens of rare ginsenosides, and the biological effects of these rare ginsenosides are different, and the biological effects of some rare ginsenosides are even antagonistic to each other. Therefore, such ginseng fermentation products are more like "panacea", which may have some effects in aspects such as whitening and enhancing the body's immunity, but there is no particularly clear and prominent effect in any aspect.

[0007] According to literature reports, among the rare ginsenosides, the ones that can enhance immunity are ginsenosides Rk1, Rg5, Rk3, Rh4 and Rg3. Summary of the invention

[0008] In view of the technical problems existing in the above-mentioned prior art, the purpose of the present invention is to provide a ginseng fermentation product in which rare ginsenosides for enhancing immunity are dominant (accounting for more than 85% by weight of all ginsenosides), an immunity enhancing composition using the ginseng fermentation product as a raw material, and a preparation method and application thereof.

[0009] The inventors have found through literature research that among the rare ginsenosides, ginsenosides Rk1, Rg5, Rk3, Rh4 and Rg3 are the ones that can enhance immunity. Based on this, it can be considered that if the total content of the above five ginsenosides in ginseng fermentation products can be increased, ginseng fermentation products with enhanced immunity enhancement effects can be prepared.

[0010] However, the technical difficulty is that the use of microorganisms (probiotics) to ferment ginseng matrix to produce rare ginsenosides will result in ginseng fermentation products containing a variety of rare ginsenosides, which usually include more than a dozen or even dozens of rare ginsenosides. The biological effects of these rare ginsenosides are different, and it is difficult to obtain a ginseng fermentation product in which one or several rare ginsenosides with a specific effect are dominant.

[0011] Furthermore, the inventors have found through in-depth research that fermenting ginseng matrix with a specific bacterial combination of Lactobacillus plantarum, Lactobacillus casei, Bifidobacterium animalis, Lactobacillus fermented mucus, and Lactobacillus paracasei can produce a ginseng fermentation product in which five ginsenosides with immunity-enhancing effects, namely Rk1, Rg5, Rk3, Rh4, and Rg3, are dominant (more than 85% by weight). Furthermore, the ginseng fermentation product is used as a raw material to prepare an immunity-enhancing composition with an immunity-enhancing effect.

[0012] The technical solution of the present invention includes:

[0013] 1. A ginseng fermentation product obtained by fermenting a ginseng fermentation substrate using a microbial strain, comprising five ginsenosides, namely, Rk1, Rg5, Rk3, Rh4 and Rg3, wherein the total content of the five ginsenosides is 85% by weight or more, and the total amount of ginsenosides in the ginseng fermentation product is 100% by weight.

[0014] The ginseng fermentation product may be liquid or solid or a solid-liquid mixture, for example, ginseng fermentation liquid or ginseng fermentation powder.

[0015] In the ginseng fermentation product of the present invention, the amount of the five rare ginsenosides is absolutely dominant, and thus the content of each of the above rare ginsenosides is also quite high. Therefore, in addition to enhancing immunity, the rare ginsenosides can also have the clearly prominent effects of any of the above rare ginsenosides, or the common effects of any number of ginsenosides.

[0016] 2-1. The ginseng fermentation product according to item 1, wherein the content of ginsenoside Rk1 is 20% by weight or more.

[0017] Ginsenoside Rk1 also has the effects of protecting liver, anti-tumor, anti-inflammatory, anti-platelet aggregation activity, kidney protection, etc. Therefore, the ginseng fermentation product of the present invention can also have the above-mentioned obvious effects.

[0018] 2-2. The ginseng fermentation product according to item 1, wherein the content of ginsenoside Rg3 is 25% by weight or more.

[0019] Ginsenoside Rg3 also has the effects of anti-tumor, improving arteriosclerosis, etc. Therefore, the ginseng fermentation product of the present invention can also have the above-mentioned obvious effects.

[0020] 2-3. The ginseng fermentation product according to item 1, wherein the content of ginsenoside Rg5 is 15% by weight or more.

[0021] Ginsenoside Rg5 also has anti-tumor, anti-inflammatory, kidney protection, hypoglycemic and other effects. Therefore, the ginseng fermentation product of the present invention can also have the above-mentioned obvious effects.

[0022] 2-4. The ginseng fermentation product according to item 1, wherein the content of ginsenoside Rk3 is 3% by weight or more.

[0023] Ginsenoside Rk3 also has anti-inflammatory, anti-tumor, hypoglycemic and other effects. Therefore, the ginseng fermentation product of the present invention can also have the above-mentioned obvious effects.

[0024] 3. The ginseng fermentation product according to item 1, wherein the content of ginsenoside Rh4 is 5% by weight or more.

[0025] Ginsenoside Rh4 also has the effects of anti-inflammatory, lowering blood sugar, improving anemia, etc. Therefore, the ginseng fermentation product of the present invention can also have the above-mentioned obvious effects.

[0026] 4. A bacterial composition, which is composed of Lactobacillus plantarum, Lactobacillus casei, Bifidobacterium animalis, Lactobacillus mucosa fermentation and Lactobacillus paracasei; wherein,

[0027] The plant lactobacillus is plant lactobacillus (Lactobacillus plantarum) GBFG 61, which is deposited in the General Microbiological Center of China Microbiological Culture Collection Administration, with a deposit number of CGMCC No. 31583 and a deposit date of August 14, 2024;

[0028] The animal bifidobacterium is animal bifidobacterium (Bifidobacterium animalis) GBFG 52, which is deposited in the General Microbiological Center of China Microbiological Culture Collection Administration, with a deposit number of CGMCC No.31582 and a deposit date of August 14, 2024;

[0029] The Lactobacillus paracasei is Lactobacillus paracasei GBFG34, which is deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration, with a deposit number of CGMCC No.31581 and a deposit date of August 14, 2024;

[0030] The fermented mucus lactobacillus is fermented mucus lactobacillus (Limosilactobacillus fermentum) PG-LFF246; and

[0031] The Lactobacillus casei is Lactobacillus casei PG-LCG139.

[0032] 5. The bacterial composition according to item 4, which can be used to ferment ginseng fermentation substrate to produce rare ginsenosides.

[0033] Here, the ginseng fermentation matrix refers to a fermentation matrix containing ginseng fines. The ginseng fines are obtained by reducing ginseng, preferably by physical methods such as extrusion, cutting, grinding, etc. The ginseng fines can be, for example, ginseng powder. The ginseng fermentation matrix can also contain other components commonly contained in the fermentation matrix, such as nitrogen source, carbon source, inorganic salt, pH regulator, etc.

[0034] 6. The bacterial composition according to item 4, wherein the dry weight ratio of Lactobacillus plantarum, Lactobacillus casei, Bifidobacterium animalis, Lactobacillus mucinous fermentation and Lactobacillus paracasei is 1:1-2:1.5-3:1.4-2.8:1.8-3.

[0035] 7. A method for preparing a ginseng fermentation product, comprising inoculating a bacterial composition into a ginseng fermentation substrate, and fermenting at 32°C to 40°C, preferably 37°C to 39°C, for 8 to 72 hours, preferably 40 to 60 hours, more preferably 45 to 50 hours, to obtain a ginseng fermentation product containing rare ginsenosides;

[0036] Wherein, the bacterial composition is the bacterial composition described in any one of items 4 to 6.

[0037] 8. The preparation method according to item 7, further comprising sterilizing the ginseng fermentation product.

[0038] 9. The preparation method according to item 7, wherein the ginseng fermentation product is the ginseng fermentation product according to item 1.

[0039] 10. The preparation method according to item 7, wherein the ginseng fermentation product is ginseng fermentation liquid or ginseng fermentation powder.

[0040] 11. A ginseng fermentation product prepared by the preparation method described in any one of items 7 to 10.

[0041] 12. An immunity enhancing composition comprising the ginseng fermentation product according to item 1 or 11.

[0042] 13. The immune-enhancing composition according to item 12, further comprising a refreshing or anti-fatigue ingredient, which also has an anti-fatigue effect.

[0043] 14. The immunity enhancing composition according to item 13, wherein the refreshing or anti-fatigue ingredient comprises one or two selected from jasmine tea powder and taurine.

[0044] 15. The immunity enhancing composition according to item 12, comprising the ginseng fermentation product, taurine and jasmine tea powder in a weight ratio of 1:(0.2-1.0):(0.5-1.5), the ginseng fermentation product being calculated by dry weight.

[0045] 16. The immune-enhancing composition according to item 15, wherein the weight ratio of the ginseng fermentation product, taurine and jasmine tea powder is 1:(0.4-0.7):(0.8-1.2).

[0046] 17. The immunity enhancing composition according to item 16, wherein the weight ratio of the ginseng fermentation product, taurine and jasmine tea powder is 1:0.6:1.

[0047] 18. The immunity enhancing composition according to item 12, which is in a beverage type.

[0048] The immune-enhancing composition may further include one or more of sugars, sugar alcohols, non-sugar and non-sugar alcohol sweeteners, pectin, soluble dietary fiber, salts, other carbohydrates, carbon dioxide or other food additives. Among them, the sugars include one or more of glucose, sucrose or fructose; the non-sugar and sugar alcohol sweeteners include one or more of stevia, aspartame, sucralose, monk fruit, glycyrrhizin, betaine or saccharin sodium; the sugar alcohols include one or more of xylitol, erythritol, maltitol, isomalt or sorbitol; the salts include one or more of sodium salt, potassium salt, phosphate; the other carbohydrates include oligosaccharides, starch and / or soluble and insoluble dietary fiber; the other food additives include one or more of flavors, pigments, malic acid, citric acid, sodium citrate, sorbic acid, potassium sorbate, benzoic acid, sodium benzoate, edible gum base, sucrose esters, soybean lecithin, sodium alginate, and the flavors and pigments are artificially synthesized or natural; the caffeine comes from cocoa, coffee, guarana, tea or chocolate.

[0049] 19. A method for preparing an immunity enhancing composition according to item 12, comprising:

[0050] The ginseng fermentation product is prepared by the method for preparing the ginseng fermentation product described in any one of items 6 to 9; and

[0051] The ginseng fermentation product is mixed with other ingredients of the immunity enhancing composition to prepare the immunity enhancing composition. BRIEF DESCRIPTION OF THE DRAWINGS

[0052] Figure 1 This is a high performance liquid chromatogram showing the determination of rare ginsenosides in fermented ginseng powder 1.

[0053] Figure 2 This is a high performance liquid chromatogram for the determination of rare ginsenosides in comparative fermented ginseng powder 3. DETAILED DESCRIPTION

[0054] The present invention is described in detail below in conjunction with specific embodiments. In the absence of conflict, the scientific terms in this specification have the meanings commonly understood by those skilled in the art. If there is a conflict, the definitions in this specification shall prevail.

[0055] Example

[0056] The plant lactobacillus (Lactobacillus plantarum) GBFG 61 is a lactobacillus strain that produces β-glucosidase and is isolated and cultivated from the soil of the ginseng planting base in Fusong County, Baishan City, Jilin Province. The plant lactobacillus strain is named GBFG 61, which is deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration Committee, with a deposit number of CGMCC No.31583, a deposit date of August 14, 2024, and a deposit address of the depository: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing; Postal code of the depository: 100101.

[0057] The animal Bifidobacterium (Bifidobacterium animalis) GBFG 52 is a bifidobacterium strain producing β-glucosidase isolated and cultivated from the soil of the ginseng planting base in Changbai County, Changbai Korean Autonomous County, Baishan City, Jilin Province. The animal Bifidobacterium was named GBFG 52, which is deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration Committee, and the deposit number is CGMCC No.31582. The deposit date is August 14, 2024, and the deposit address is: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences; Postal code of the deposit agency: 100101.

[0058] The Lactobacillus paracasei GBFG34 is a lactobacillus strain that produces β-glucosidase isolated and cultivated from the soil of the ginseng planting base in Ji'an City (County) of Tonghua City, Jilin Province. The Lactobacillus paracasei was named GBFG 34, which is deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration Committee, and the deposit number is CGMCC No.31581. The deposit date is August 14, 2024, and the deposit address is: Institute of Microbiology, Chinese Academy of Sciences, No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing; Postal code of the deposit agency: 100101.

[0059] The Lactobacillus casei BL23 was purchased from Beijing Crespo Biotechnology Co., Ltd.

[0060] The fermented Lactobacillus fermentum PG-LFF246, Lactobacillus casei PG-LCG139, Lactobacillus plantarum PG-LPZ131, Bifidobacterium animalis PG-BAD260, and Lactobacillus paracasei PG-LPF109 were all purchased from Shandong Pingao Pharmaceutical Co., Ltd.

[0061] The fermented mucus lactobacillus CECT5716 was purchased from Shaanxi Angxu Biotechnology Co., Ltd.

[0062] Example 1 The preparation of the immune-enhancing ginseng fermented liquid rich in rare ginsenosides comprises the following steps:

[0063] The fermentation medium consisted of the following components: glucose 15 g / L, yeast powder 10 g / L, citric acid 1 g / L, manganese sulfate 0.05 g / L, magnesium sulfate 0.1 g / L, ginseng powder 120 g / L, water, adjusted to pH 5.0, and sterilized.

[0064] The fermentation medium is inoculated with an activated bacterial composition at 2 weight % (wherein the dry weight ratio of Lactobacillus plantarum GBFG61: Lactobacillus casei PG-LCG139: Bifidobacterium animalis GBFG52: Limosilactobacillus fermentum PG-LFF246: Lactobacillus paracasei GBFG34 is 1:1.3:2:1.8:2), and fermented at 38° C. for 48 hours to obtain a fermentation liquid; the fermentation liquid is sterilized at 121° C. for 21 minutes, and concentrated under reduced pressure to obtain a concentrated liquid; the concentrated liquid is spray-dried to obtain a dry paste powder; the dry paste powder is crushed and passed through an 80-mesh sieve to obtain a fine powder, namely, fermented ginseng powder 1.

[0065] Example 2

[0066] Fermentation preparation was carried out in the same manner as in Example 1 to obtain fermented ginseng powder 2, except that in the activated bacterial composition, the dry weight ratio of Lactobacillus plantarum GBFG61: Lactobacillus casei PG-LCG139: Bifidobacterium animalis GBFG52: Limosilactobacillus fermentum PG-LFF246: Lactobacillus paracasei GBFG34 was 1:2:3:2.8:3.

[0067] Example 3

[0068] Fermentation preparation was carried out in the same manner as in Example 1 to obtain fermented ginseng powder 3, except that in the activated bacterial composition, the dry weight ratio of Lactobacillus plantarum GBFG61: Lactobacillus casei PG-LCG139: Bifidobacterium animalis GBFG52: Limosilactobacillus fermentum PG-LFF246: Lactobacillus paracasei GBFG34 was 1:1:1.5:1.4:1.8.

[0069] Comparative Example 1

[0070] The fermentation preparation was carried out in the same manner as in Example 1 to obtain fermented ginseng powder 1, except that the Bifidobacterium animalis GBFG 52 was replaced by Bifidobacterium animalis PG-BAD260.

[0071] Comparative Example 2

[0072] The fermentation preparation was carried out as in Example 1 to obtain comparative fermented ginseng powder 3, except that the Lactobacillus paracasei GBFG34 was replaced by Lactobacillus paracasei PG-LPF109.

[0073] Comparative Example 3

[0074] The fermentation preparation was carried out as in Example 1 to obtain comparative fermented ginseng powder 4, except that the Lactobacillus plantarum GBFG61 was replaced by Lactobacillus plantarum PG-LPZ131.

[0075] Example 4 Determination of rare ginsenoside content in fermented ginseng powder

[0076] The above fermented ginseng powders were dissolved in methanol to prepare 80 mg / mL, and 1 mL of the solution was taken and filtered with a 0.45 μm microporous filter membrane to obtain a clear solution. The total ginsenoside content and the content of various rare ginsenosides therein were determined, and the total amount of the five rare ginsenosides Rk1, Rg5, Rk3, Rh4 and Rg3 was calculated (weight %, with the total ginsenoside amount as 100 weight %). The results are shown in Table 1.

[0077] The detection method of total saponins was carried out according to the method for determining the content of total ginsenosides in the Pharmacopoeia of the People's Republic of China (2020 edition). 48 H 82 O 18 )count.

[0078] The detection method of rare ginsenosides is carried out according to the method for determining the content of rare ginsenosides in T / CNHFA 001-2021.

[0079] As an example, the HPLC chromatograms for determining the total contents of five ginsenosides, Rk1, Rg5, Rk3, Rh4 and Rg3, in the fermented ginseng powders prepared in Example 1 and Comparative Example 3 are shown in FIG. Figure 1 and Figure 2 shown.

[0080] Table 1. Content of rare ginsenosides with immune-enhancing effects in fermented ginseng powder

[0081]

[0082] As can be seen from Table 1, when the strain combination of Lactobacillus plantarum GBFG61: Lactobacillus casei PG-LCG139: Bifidobacterium animalis GBFG52: Limosilactobacillus fermentum PG-LFF246: Lactobacillus paracasei GBFG34 of the present invention is selected, the rare ginsenosides that enhance immunity in the obtained fermented ginseng powder have an absolute advantage, which suggests that the obtained fermented ginseng powder has an enhanced immunity-enhancing effect.

[0083] Example 5 Verification of the Immunity Enhancement Effect of Fermented Ginseng Powders

[0084] The fermented ginseng powder prepared in the above Example 1 and Comparative Example 3 was selected to conduct an immunity enhancement efficacy evaluation test.

[0085] 1.1 Experimental animals

[0086] Kunming mice, 18-22 g, male, 15 mice per group.

[0087] 1.2 Grouping and test sample administration time

[0088] The experiment set up two treatment groups and one control group. The dosage of fermented ginseng powder 1 and comparison fermented ginseng powder 3 were both 250 mg / kg.bw. The control group was given normal saline with the same dosage as the treatment group. The sample was administered for 30 days.

[0089] 1.3 Experimental indicators and methods

[0090] 1.3.1 Delayed-type hypersensitivity (DTH)

[0091] The DTH (ear swelling method) of mice was induced by dinitrofluorobenzene.

[0092] DNFB solution: It should be freshly prepared. Weigh 50 mg of DNFB and place it in a clean and dry vial. Pour 5 mL of the pre-prepared acetone sesame oil solution (acetone: sesame oil = 1:1) into the vial, cover the bottle stopper and seal it with tape. After mixing, use a 250 μL syringe to take it through the bottle cap.

[0093] Sensitization: After the administration of the test sample, the abdominal skin of each mouse was depilated or shaved with barium sulfide, with an area of ​​about 3cm×3cm, and 50μL of DNFB solution was evenly applied for sensitization.

[0094] Production and determination of DTH: 5 days after sensitization, 10 μL of DNFB solution was evenly applied to the right ears (both sides) of mice for attack. 24 hours after the attack, the mice were killed by cervical dislocation, and the left and right ear shells were cut off. The ear pieces with a diameter of 8 mm were removed with a punch and weighed.

[0095] The difference between the left and right ear weights indicates the degree of DTH. If the weight difference of the test sample group is significantly higher than that of the control group, the test result can be judged to be positive.

[0096] 1.3.2 Carbon clearance experiment in mice

[0097] Ink for injection: Dilute the stock solution of India ink 3 to 4 times with normal saline.

[0098] Na2CO3 solution: Take 0.1g Na2CO3 and add distilled water to 100mL.

[0099] Ink injection: Diluted India ink (10 ml / kg) was injected into the tail vein of the mouse according to its body weight, and the time was immediately started after the ink was injected.

[0100] Determination: 20 μL of blood was collected from the medial canthal venous plexus at 2 and 10 minutes after the injection of ink, and immediately added to 2 mL of 0.1% Na2CO3 solution. The optical density (OD) was measured at a wavelength of 600 nm, and the Na2CO3 solution was used as a blank control. The mice were killed, and the liver and spleen were taken out. The blood stains on the surface of the organs were blotted with filter paper and weighed separately.

[0101] The phagocytic index is used to represent the carbon clearance ability of mice. The phagocytic index a is calculated according to the following formula.

[0102]

[0103] The phagocytic index of the test sample group was significantly higher than that of the control group, which indicated that the experimental result was positive.

[0104] 1.4 Experimental Results

[0105] After the experiment, the DTH degree, clearance index and phagocytosis index of each group of mice were counted according to the methods of 1.3.1 and 1.3.2. The specific results are shown in Table 4:

[0106] Table 2 Immunity enhancement efficacy indexes of different fermented ginseng powders

[0107] Group DTH degree / g Clearance index κ Phagocytic index α Control group 0.38±0.052 0.012±0.0012 3.65±0.52 Fermented ginseng powder1 0.75±0.061**# 0.068±0.0052**# 6.79±0.61**# Comparison of fermented ginseng powder 3 0.47±0.051* 0.027±0.0017* 4.18±0.22*

[0108] *Significant difference compared with the control group (P<0.05); **Significant difference compared with the control group (P<0.01); #Significant difference compared with the three groups of fermented ginseng powder (P<0.01)

[0109] It can be seen from Table 2 that from the three important indicators of immunity, namely, DTH degree, carbon clearance capacity κ and phagocytic index α, compared with the control group, the fermented ginseng powder 1 and the comparative fermented ginseng powder 3 both have certain immunity enhancement effects, but the immunity enhancement effect of the fermented ginseng powder 1 within the scope of the present invention is significantly stronger than that of the comparative fermented ginseng powder 3 not within the scope of the present invention.

[0110] Example 6 Preparation of Immunity Enhancement and Anti-fatigue Composition

[0111] The fermented ginseng powder 1 prepared in the above Example 1, taurine and jasmine tea powder were mixed at a weight ratio of 1:0.6:1, and the mixture was passed through a 200-mesh sieve to obtain an immunity enhancing and anti-fatigue composition 1.

[0112] Example 7: Verification of the Immunity-Enhancing and Anti-Fatigue Composition

[0113] The immunity enhancing efficacy of the immunity enhancing and anti-fatigue composition 1 was verified as in the above-mentioned Example 5. The experimental results are shown in Table 3.

[0114] Table 3 Immunity-enhancing efficacy indexes of the immunity-enhancing and anti-fatigue composition 1

[0115]

[0116]

[0117] **Significantly different from the control group (P<0.01)

[0118] As shown in Table 3, the DTH level, carbon clearance capacity κ and phagocytic index α of the mice in the immunity enhancing and anti-fatigue composition group 1 were statistically different from those of the mice in the control group (P < 0.01), which shows that the immunity enhancing and anti-fatigue composition of the present invention has a good immunity enhancing effect.

[0119] Example 8: Verification of the Anti-fatigue Efficacy of the Immunity Enhancement and Anti-fatigue Composition

[0120] The above-mentioned immunity enhancing and anti-fatigue composition 1 was selected to carry out an anti-fatigue efficacy evaluation experiment.

[0121] 1.1 Experimental animals

[0122] Kunming mice, 18-22 g, male, 15 mice per group.

[0123] 1.2 Dosage groups and test sample administration time

[0124] The experiment set up a drug administration group and a control group, the drug administration amount was 300 mg / kg.bw, the control group was given normal saline, the dosage was the same as the composition, and the sample administration time was 45 days.

[0125] 1.3 Experimental methods

[0126] 30 minutes after the first administration of the test sample, place the mouse with a lead sheet of 5% of its body weight at the base of its tail in a swimming box. The water depth should be no less than 30 cm and the water temperature should be 25℃±1.0℃. The time from the start of swimming to the death of the mouse is recorded, i.e., the weight-bearing swimming time of the mouse.

[0127] 1.4 Experimental Results

[0128] The experimental results showed that the weighted swimming time of the mice in the blank control group was 5.11±1.02 minutes on average, while the weighted swimming time of the mice in the immunity enhancing and anti-fatigue composition 1 group was 13.9±2.28 minutes on average. This indicates that the immunity enhancing and anti-fatigue composition of the present invention has a very good anti-fatigue effect.

[0129] The above embodiments are preferred implementations of the present invention, but the implementations of the present invention are not limited to the above embodiments. For ordinary technicians in this technical field, other different forms of changes or modifications can be made without departing from the principles of the present invention. The derived changes are still within the scope of the creative protection of the present invention.

[0130] Industrial Applicability

[0131] The present invention provides an immunity enhancing composition comprising a ginseng fermentation product with enhanced immunity enhancing effect, a preparation method and application thereof.

Claims

1. An immunity enhancing composition comprising a ginseng fermentation product; wherein: The ginseng fermentation product is obtained by fermenting a ginseng fermentation substrate using a microbial strain, and contains five ginsenosides, namely, Rk1, Rg5, Rk3, Rh4 and Rg3, and the total content of the five ginsenosides is more than 85% by weight, with the total amount of ginsenosides in the ginseng fermentation product being 100% by weight.

2. The immunity enhancing composition according to claim 1, which is in liquid or solid form.

3. The immune-enhancing composition according to claim 1, further comprising a refreshing or anti-fatigue component, and further having an anti-fatigue effect.

4. The immunity enhancing composition according to claim 3, comprising the ginseng fermentation product, taurine and jasmine tea powder in a weight ratio of 1:(0.2-1.0):(0.5-1.5), and the ginseng fermentation product is calculated by dry weight.

5. The method for preparing the immunity enhancing composition according to claim 1, comprising: The preparation of the ginseng fermentation product comprises inoculating the bacterial composition into a ginseng fermentation substrate, and fermenting at 32°C to 40°C, preferably 37°C to 39°C for 8 to 72 hours, preferably 40 to 60 hours, and more preferably 45 to 50 hours, thereby obtaining a ginseng fermentation product containing rare ginsenosides; and Mixing the ginseng fermentation product with other ingredients of the immunity enhancing composition to prepare the immunity enhancing composition; The bacterial composition is composed of Lactobacillus plantarum, Lactobacillus casei, Bifidobacterium animalis, Lactobacillus mucosa fermentation and Lactobacillus paracasei; wherein, The plant lactobacillus is plant lactobacillus (Lactobacillus plantarum) GBFG 61, which is deposited in the General Microbiological Center of China Microbiological Culture Collection Administration, with a deposit number of CGMCC No. 31583 and a deposit date of August 14, 2024; The animal bifidobacterium is animal bifidobacterium (Bifidobacterium animalis) GBFG 52, which is deposited in the General Microbiological Center of China Microbiological Culture Collection Administration, with a deposit number of CGMCC No.31582 and a deposit date of August 14, 2024; The Lactobacillus paracasei is Lactobacillus paracasei GBFG 34, which is deposited in the General Microbiology Center of China Microbiological Culture Collection Administration, with a deposit number of CGMCC No.31581 and a deposit date of August 14, 2024; The fermented mucus lactobacillus is fermented mucus lactobacillus (Limosilactobacillus fermentum) PG-LFF246; and The Lactobacillus casei is Lactobacillus casei PG-LCG139.

6. The preparation method according to claim 5, wherein: The dry weight ratio of the plant lactobacillus, the casei lactobacillus, the animal bifidobacterium, the fermented mucus lactobacillus and the paracasei lactobacillus in the bacterial composition is 1:1-2:1.5-3:1.4-2.8:1.8-3.

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