A composition containing collagen and its application in food preparation

Abstract

The invention provides a composition containing collagen, which comprises the following components in parts by weight: 15-30 parts of a collagen mixture, 10-20 parts of a plant extract, 2-4 parts of a vitamin mixture, 1-3 parts of D-ribose and 3-7 parts of glucosamine; the collagen mixture is fish scale collagen, deer collagen and bovine bone collagen; the plant extract is an ethanol extract obtained by mixing reed root, cherry, grape, tulip tree, blueberry, vine of green wind and arrowhead; the vitamin mixture is a mixture of vitamin B, vitamin C and vitamin E; the composition is applied to food to achieve obvious skin improvement and anti-aging effects.

Description

Technical Field

[0001] The present invention relates to the technical field of collagen, and in particular to a composition containing collagen and application thereof in preparing food. Background Art

[0002] Collagen is a biological macromolecular substance, and its diversity and complexity of structure make it applicable in many fields. Collagen not only has the effect of beauty and fitness, but also has good biocompatibility, biodegradability and biological activity. It is easily absorbed by the human body, can promote cell survival and growth, promote platelet aggregation, etc., and is widely used in the food field.

[0003] Patent CN118547035A provides a fish collagen peptide and its composition and its application in preparing a drink for improving skin. The composite drink obtained by using fish collagen peptide as raw material and compounding three kinds of fresh fruits, kumquat, lemon and orange, has good stability, light yellow color, no browning, and uniform color. However, the existing collagen used in food is of a single variety, with low purity and extraction rate, which fails to effectively exert the efficacy of collagen and also affects the effect of food. Summary of the invention

[0004] The purpose of the present invention is to provide a collagen-containing composition and a preparation method thereof, which is mainly composed of a collagen mixture and a plant extract, supplemented with ingredients such as a vitamin mixture, D-ribose and glucosamine, to obtain a collagen-containing composition capable of improving skin and aging.

[0005] In order to achieve the above object, the present invention provides the following technical solutions: A collagen-containing composition comprises the following components in parts by weight: 15-30 parts of a collagen mixture, 10-20 parts of a plant extract, 2-4 parts of a vitamin mixture, 1-3 parts of D-ribose and 3-7 parts of glucosamine.

[0006] Preferably, the collagen mixture is fish scale collagen, deer collagen and bovine bone collagen, and the mass ratio of the three is 1:2-3:1.3-1.8.

[0007] Preferably, the molecular weight of the fish scale collagen is distributed in the range of 2000-2500 Daltons, the molecular weight of the deer collagen is distributed in the range of 900-1300 Daltons, and the molecular weight of the bovine bone collagen is distributed in the range of 800-1000 Daltons.

[0008] Preferably, the plant extract is an ethanol extract of a mixture of reed root, cherry, grape, tulip tree, blueberry, Caulis Sinomenii and Sagittaria.

[0009] Preferably, the vitamin mixture is a mixture of vitamin B, vitamin C and vitamin E, and the mass ratio of the three is 1:1.3-1.5:1.2-1.8.

[0010] A method for preparing a composition containing collagen comprises the following steps: S1: Mix fish scale collagen, deer collagen and bovine bone collagen in a mass ratio of 1:2-3:1.3-1.8, and stir at room temperature for 20-40 minutes to obtain a collagen mixture; S2: mixing reed root, cherry, grape, tulip tree, blueberry, scutellaria baicalensis and arrowhead, adding ethanol solution, stirring and refluxing at a temperature of 90-110° C. for 1.5-3.5 h, filtering and separating to obtain plant extract; S3: Mix vitamin B, vitamin C and vitamin E in a mass ratio of 1:1.3-1.5:1.2-1.8, and stir and mix at a temperature of 40-50° C. for 30-50 minutes to obtain a vitamin mixture; S4: In parts by weight, 15-30 parts of the collagen mixture described in step S1, 10-20 parts of the plant extract described in step S2, 2-4 parts of the vitamin mixture described in step S3, 1-3 parts of D-ribose and 3-7 parts of glucosamine are mixed, stirred and reacted at a temperature of 60-80° C. for 20-40 minutes, and then ultrasonically treated for 15-25 minutes to obtain a collagen-containing composition.

[0011] Preferably, the fish scale collagen preparation process of step S1 is as follows: by weight, 10-20 parts of washed fish scales are immersed in 60-100 parts of 0.3-0.6 mol / L sodium hydroxide solution for 2-4 hours, removed and immersed in 50-90 parts of 0.5-1 mol / L hydrochloric acid solution for 1-2 hours, and washed until neutral; then 80-120 parts of deionized water and 2-4 parts of collagenase are added, and the reaction is carried out at a temperature of 100-120°C for 30-50 minutes; the filtrate obtained by filtration is treated with a nanofilter and an ion exchange resin, and dried at 80-100°C to obtain fish scale collagen.

[0012] Preferably, the deer collagen preparation process of step S1 is as follows: by weight, immerse 10-20 parts of washed deer bones in 40-80 parts of 0.8-1.3 mol / L sodium hydroxide solution for 1-2 hours, remove and immerse in 60-80 parts of 1.5-2.5 mol / L hydrochloric acid solution for 1-2 hours, and wash until neutral; then add 70-100 parts of deionized water and 1-3 parts of rennet, react at a temperature of 100-120°C for 45-75 minutes; treat the filtrate obtained by filtration with a nanofilter and an ion exchange resin, and dry at 80-100°C to obtain deer collagen.

[0013] Preferably, the bovine collagen preparation process of step S1 is as follows: by weight, 15-25 parts of washed bovine bones are immersed in 60-90 parts of 2-4 mol / L sodium hydroxide solution for 1-4 hours, taken out and immersed in 80-100 parts of 2-3 mol / L sulfuric acid solution for 1.5-2.5 hours, and washed until neutral; then 75-95 parts of deionized water and 2-4 parts of tissue protease are added, and the reaction is carried out at a temperature of 100-120°C for 50-70 minutes; the filtrate obtained by filtration is treated with a nanofilter and an ion exchange resin, and dried at 80-100°C to obtain bovine collagen.

[0014] Use of the collagen-containing composition prepared by any one of the above methods in food preparation.

[0015] Fish scale collagen is extracted from fish scales. Collagen is one of the most abundant proteins in animals and is essential for the structure and function of skin, bones, tendons and other tissues. Fish scale collagen has a wide range of applications in beauty, health care and other fields due to its good biocompatibility and easy absorption.

[0016] Deer collagen is extracted from specific parts of deer. Deer collagen has attracted wide attention in the fields of beauty and health care due to its unique biological activity and nutritional value. Biocompatibility: Deer collagen has a similar structure to collagen in the human body and has good biocompatibility. Absorbability: Deer collagen has a small molecular weight and is more easily absorbed and utilized by the human body. Nutritional value: It is rich in amino acids, especially glycine, proline and hydroxyproline.

[0017] Bovine collagen is extracted from bovine bones and is commonly used in food, health care and beauty products. Bovine collagen has attracted much attention due to its rich nutritional value and various health benefits.

[0018] Compared with the prior art, the advantages and beneficial effects of the present invention are: The invention uses fish scale collagen, deer collagen and bovine bone collagen as a collagen mixture, and makes the effective components of the three components work synergistically through appropriate proportions to improve the efficacy of collagen; uses reed root, cherry, grape, tulip tree, blueberry, lily of the valley and arrowhead as raw materials to prepare plant extracts, so that the obtained composition has the above-mentioned plant effective components at the same time; in addition, under the action of a mixture of vitamin B, vitamin C and vitamin E, as well as D-ribose and glucosamine, the finally prepared collagen-containing composition has good anti-skin sagging and aging effects; and is applied in food preparation to promote skin improvement through diet therapy. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] The raw materials used in the present invention are as follows: Collagenase was from Hubei Wei's Chemical Reagent Co., Ltd., catalog number 1998; ion exchange resin was from Shanghai Yuanye Biotechnology Co., Ltd., catalog number S14158; rennet was from Jiangsu Caiwei Biotechnology Co., Ltd., catalog number 5103; tissue protease was from Wuhan Feiyue Biotechnology Co., Ltd., catalog number FY-P526116; vitamin B was from Shanghai Senpeptide Biotechnology Co., Ltd., catalog number ST-H4495; vitamin C was from Shanghai Senpeptide Biotechnology Co., Ltd., catalog number ST-H4496; vitamin E was from Shanghai Senpeptide Biotechnology Co., Ltd., catalog number ST-H4510; glucosamine was from Shanghai Yuanye Biotechnology Co., Ltd., catalog number S27623; D-ribose was from Shanghai Yuanye Biotechnology Co., Ltd., catalog number S11068.

[0021] Example 1 This embodiment provides a method for preparing a composition containing collagen, comprising the following steps: S1: 10g of washed fish scales are immersed in 100g of 0.3mol / L sodium hydroxide solution for 2h, taken out and immersed in 50g of 0.5mol / L hydrochloric acid solution for 1h, washed until neutral; then 80g of deionized water and 2g of collagenase are added, and the reaction is carried out at a temperature of 100°C and a speed of 500r / min for 30min; the filtrate obtained by filtration is treated with a nanofilter and an ion exchange resin, and dried at 80°C to obtain fish scale collagen; 10 g of washed deer bones were immersed in 40 g of 0.8 mol / L sodium hydroxide solution for 1 hour, then taken out and immersed in 60 g of 1.5 mol / L hydrochloric acid solution for 1 hour, washed until neutral; then 70 g of deionized water and 1 g of rennet were added, and the reaction was carried out at a temperature of 100° C. and a speed of 550 r / min for 45 minutes; the filtrate obtained by filtration was treated with a nanofilter and an ion exchange resin, and dried at 80° C. to obtain deer collagen; 15g of washed bovine bones were immersed in 60g of 2mol / L sodium hydroxide solution for 1 hour, and then taken out and immersed in 80g of 2mol / L sulfuric acid solution for 1.5 hours to wash until neutral; then 75g of deionized water and 2g of cathepsin were added, and the reaction was carried out at a temperature of 100°C and a speed of 600r / min for 50min; the filtrate obtained by filtration was treated with a nanofilter and an ion exchange resin, and dried at 80°C to obtain bovine bone collagen; Fish scale collagen, deer collagen and bovine bone collagen were mixed in a mass ratio of 1:2:1.3, and stirred at a rate of 450 r / min for 20 min at room temperature to obtain a collagen mixture; S2: Mix 1g of Phragmites australis, 3g of cherry, 2g of grape, 1g of Liriodendron chinense, 2g of blueberry, 1g of Caulis Sinomenii and 2g of Sagittaria sagittifolia, add 80g of ethanol solution, stir and reflux at 90°C at a rate of 600r / min for 1.5h, and filter and separate to obtain the plant extract; S3: Mix vitamin B, vitamin C and vitamin E in a mass ratio of 1:1.3:1.2, and stir at a temperature of 40° C. and a speed of 400 r / min for 30 min to obtain a vitamin mixture; S4: Mix 15 g of the collagen mixture described in step S1, 10 g of the plant extract described in step S2, 2 g of the vitamin mixture described in step S3, 1 g of D-ribose and 3 g of glucosamine, stir and react at a temperature of 60°C and a speed of 500 r / min for 20 min, and then ultrasonically treat at a power of 1000 W for 15 min to obtain a collagen-containing composition.

[0022] Example 2 This embodiment provides a method for preparing a composition containing collagen, comprising the following steps: S1: 20g of washed fish scales are immersed in 100g of 0.6mol / L sodium hydroxide solution for 4h, taken out and immersed in 90g of 1mol / L hydrochloric acid solution for 2h, washed until neutral; then 120g of deionized water and 4g of collagenase are added, and the reaction is carried out at a temperature of 120°C and a speed of 300r / min for 50min; the filtrate obtained by filtration is treated with a nanofilter and an ion exchange resin, and dried at 100°C to obtain fish scale collagen; 20 g of washed deer bones were immersed in 80 g of 1.3 mol / L sodium hydroxide solution for 2 h, then taken out and immersed in 80 g of 2.5 mol / L hydrochloric acid solution for 2 h, washed until neutral; then 100 g of deionized water and 3 g of rennet were added, and the reaction was carried out at a temperature of 120° C. and a speed of 500 r / min for 75 min; the filtrate obtained by filtration was treated with a nanofilter and an ion exchange resin, and dried at 100° C. to obtain deer collagen; 25g of washed bovine bones were immersed in 90g of 4mol / L sodium hydroxide solution for 4h, then taken out and immersed in 100g of 3mol / L sulfuric acid solution for 2.5h, washed until neutral; then 95g of deionized water and 4g of cathepsin were added, and the reaction was carried out at a temperature of 120°C and a speed of 500r / min for 70min; the filtrate obtained by filtration was treated with a nanofilter and an ion exchange resin, and dried at 100°C to obtain bovine bone collagen; Fish scale collagen, deer collagen and bovine bone collagen were mixed in a mass ratio of 1:3:1.8, and stirred at a rate of 400 r / min for 40 min at room temperature to obtain a collagen mixture; S2: Mix 3g of Phragmites australis, 5g of cherry, 5g of grape, 4g of Liriodendron chinense, 4g of blueberry, 4g of Caulis Sinomenii and 5g of Sagittaria sagittifolia, add 120g of ethanol solution, stir and reflux at a temperature of 110°C and a speed of 550r / min for 3.5h, and filter and separate to obtain the plant extract; S3: Mix vitamin B, vitamin C and vitamin E in a mass ratio of 1:1.5:1.8, and stir at a temperature of 50° C. and a speed of 500 r / min for 50 min to obtain a vitamin mixture; S4: 30 g of the collagen mixture described in step S1, 20 g of the plant extract described in step S2, 4 g of the vitamin mixture described in step S3, 3 g of D-ribose and 7 g of glucosamine are mixed, stirred at a temperature of 80°C and a speed of 400 r / min for 40 min, and then ultrasonically treated at a power of 850 W for 25 min to obtain a collagen-containing composition.

[0023] Example 3 This embodiment provides a method for preparing a composition containing collagen, comprising the following steps: S1: 15g of washed fish scales were immersed in 85g of 0.4mol / L sodium hydroxide solution for 3h, then taken out and immersed in 75g of 0.7mol / L hydrochloric acid solution for 1.5h, washed until neutral; then 110g of deionized water and 3g of collagenase were added, and the reaction was carried out at a temperature of 115°C and a speed of 300r / min for 45min; the filtrate obtained by filtration was treated with a nanofilter and an ion exchange resin, and dried at 90°C to obtain fish scale collagen; 16g of washed deer bones were immersed in 70g of 1.0mol / L sodium hydroxide solution for 1.5h, then taken out and immersed in 75g of 2mol / L hydrochloric acid solution for 1.5h, washed until neutral; then 95g of deionized water and 2g of rennet were added, and the reaction was carried out at a temperature of 115°C and a speed of 400r / min for 65min; the filtrate obtained by filtration was treated with a nanofilter and an ion exchange resin, and dried at 90°C to obtain deer collagen; 22g of washed bovine bones were immersed in 80g of 3mol / L sodium hydroxide solution for 3.5h, taken out and immersed in 95g of 2.5 / L sulfuric acid solution for 2h, washed until neutral; then 90g of deionized water and 3g of cathepsin were added, reacted at a temperature of 110°C and a speed of 500r / min for 65min; the filtrate obtained by filtration was treated with a nanofilter and an ion exchange resin, and dried at 90°C to obtain bovine bone collagen; Fish scale collagen, deer collagen and bovine bone collagen were mixed in a mass ratio of 1:2.3:1.5, and stirred at a rate of 600 r / min for 35 min at room temperature to obtain a collagen mixture; S2: Mix 2g of Phragmites australis, 4g of cherry, 4g of grape, 3g of Liriodendron chinense, 3g of blueberry, 3g of Caulis Sinomenii and 3g of Sagittaria sagittifolia, add 110g of ethanol solution, stir and reflux at 100°C at a rate of 500r / min for 2.5h, and filter and separate to obtain the plant extract; S3: Mix vitamin B, vitamin C and vitamin E in a mass ratio of 1:1.4:1.6, and stir at a temperature of 45°C and a speed of 500 r / min for 30-5045 min to obtain a vitamin mixture; S4: Mix 25 g of the collagen mixture described in step S1, 15 g of the plant extract described in step S2, 3 g of the vitamin mixture described in step S3, 2 g of D-ribose and 5 g of glucosamine, stir at 70°C and 500 r / min for 35 min, and then ultrasonically treat at 850 W for 15-25 min to obtain a collagen-containing composition.

[0024] Comparative Example 1 The difference between this comparative example and Example 1 is that in step S1, only fish scale collagen is used, deer collagen and bovine bone collagen are lacking, and the other steps are the same.

[0025] Comparative Example 2 The difference between this comparative example and Example 1 is that in step S1, only deer collagen is used, and fish scale collagen and bovine bone collagen are lacking, and the other steps are the same.

[0026] Comparative Example 3 The difference between this comparative example and Example 1 is that in step S1, only bovine bone collagen is used, and fish scale collagen and deer collagen are lacking, and the other steps are the same.

[0027] Comparative Example 4 The difference between this comparative example and Example 1 is that step S2 is missing, that is, there is no plant extract, and the other steps are the same.

[0028] Comparative Example 5 The difference between this comparative example and Example 1 is that step S3 is missing, that is, there is no vitamin mixture, and the other steps are the same.

[0029] Comparative Example 6 The difference between this comparative example and Example 1 is that D-ribose and 3 g of glucosamine are missing in step S4, and the other steps are the same.

[0030] Performance Test: The ingredients are weighed: 2% salt, 1% sugar, 0.2% monosodium glutamate, 0.8% chicken powder and 76% rice flour, and the water content of the ingredients is 25%; the ingredients, amino acid-bound calcium, and the collagen-containing compositions of various embodiments and comparative examples are mixed according to a mass ratio of 2:3:100, the pH value is adjusted to 7.0, and the mixture is extruded at a pressure of 0.3 MPa and a temperature of 180° C. to form the mixture; the ratio of shear force to kneading force is about 1:1.25, the particle size equivalent diameter is 3-6 mm, and the mixture is dried at 80° C. until the water content reaches below 13%, thereby obtaining high-calcium and high-collagen gel rice grains; and the mixture is puffed at a pressure of 0.15 MPa and a temperature of 170° C. for 1.5 seconds to obtain edible puffed rice cakes.

[0031] Effect verification 15 volunteers aged between 40 and 50 were recruited, including 8 men and 7 women, and were asked to eat the foods prepared in the embodiments and comparative examples for one month, 100 g per day, and live a normal life according to their previous living habits and rules during this period.

[0032] Before the test, blood was drawn to detect the biochemical indicators of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in the serum; these indicators were tested again after the test time.

[0033] MDA is the final product of lipid peroxidation. It can combine with biological macromolecules such as proteins, nucleic acids, and lipids to form insoluble substances, causing damage to various macromolecules in the body. It is an important indicator for measuring lipid peroxidation during the aging process of the body.

[0034] SOD is one of the important antioxidant enzymes in the body, which can remove superoxide anion free radicals, thereby protecting the body's cells and tissues from damage by free radicals and delaying the aging process of the body. It is also found that the activity of antioxidant enzymes such as SOD is low in aging organisms.

[0035] GSH-Px is an important antioxidant enzyme in the body, which can indirectly reduce the formation of lipid peroxides in the body. The activity of this enzyme also decreases with age.

[0036] T-AOC is the total level of various antioxidant macromolecules, antioxidant small molecules and enzymes in the body, which reflects the total antioxidant capacity of the system. In the study of aging mechanism, it is believed that the main reason for the aging of the body is the decrease in the activity of the body's own antioxidant system with the increase of age. There are many antioxidants in the body, including antioxidant macromolecules, antioxidant small molecules and enzymes, which can remove various reactive oxygen species produced in the body to prevent the generation of oxidative stress induced by reactive oxygen species. Antioxidants decrease with age.

[0037] Mouse animal experiment: 90 16-month-old ICR female mice weighing 33-45g were selected. 90 naturally aged mice were randomly divided into 9 groups, namely, Examples 1-3, Comparative Examples 1-6 Comparative Examples (0.1g / kg), and were continuously gavaged for 60 days. After gavage for 60 days, the eyeballs were removed to obtain sufficient serum samples and stored at -20°C for later use. The serum MDA content, SOD, GSH-Px and T-AOC activities were detected by the kit method. The results of the anti-skin aging effect test are as follows:

[0038] It can be seen from the above performance test results that Examples 1-3 effectively promote the vitality of each effective ingredient and the absorption and utilization efficiency of nutrients; contain a variety of nutrients required by the human body, can effectively remove free radicals in the body, improve the body's immunity and health, and thus achieve the purpose of beauty and anti-aging. In particular, the comprehensive performance of Example 3 is the most outstanding. However, because Comparative Examples 1-6 did not adopt the necessary technical solutions, they were significantly worse than the examples in the corresponding performance tests. The above experimental results further prove the importance of the technical solutions defined in the present invention for its technical effects.

[0039] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A composition containing collagen, characterized in that: The invention comprises the following components in parts by weight: 15-30 parts of collagen mixture, 10-20 parts of plant extract, 2-4 parts of vitamin mixture, 1-3 parts of D-ribose and 3-7 parts of glucosamine.

2. A collagen-containing composition according to claim 1, characterized in that: The collagen mixture is fish scale collagen, deer collagen and bovine bone collagen, and the mass ratio of the three is 1:2-3:1.3-1.

8.

3. A collagen-containing composition according to claim 2, characterized in that: The molecular weight of the fish scale collagen is distributed in the range of 2000-2500 Daltons, the molecular weight of the deer collagen is distributed in the range of 900-1300 Daltons, and the molecular weight of the bovine bone collagen is distributed in the range of 800-1000 Daltons.

4. A collagen-containing composition according to claim 1, characterized in that: The plant extract is an ethanol extract obtained by mixing rhizoma reedii, cherry, grape, tulip tree, blueberry, celandine and sagittaria.

5. The collagen-containing composition according to claim 1, characterized in that: The vitamin mixture is a mixture of vitamin B, vitamin C and vitamin E, and the mass ratio of the three is 1:1.3-1.5:1.2-1.

8.

6. A method for preparing a composition containing collagen, characterized in that: The steps include: S1: Mix fish scale collagen, deer collagen and bovine bone collagen in a mass ratio of 1:2-3:1.3-1.8, and stir at room temperature for 20-40 minutes to obtain a collagen mixture; S2: mixing reed root, cherry, grape, tulip tree, blueberry, scutellaria baicalensis and arrowhead, adding ethanol solution, stirring and refluxing at a temperature of 90-110° C. for 1.5-3.5 h, filtering and separating to obtain plant extract; S3: Mix vitamin B, vitamin C and vitamin E in a mass ratio of 1:1.3-1.5:1.2-1.8, and stir and mix at a temperature of 40-50° C. for 30-50 minutes to obtain a vitamin mixture; S4: In parts by weight, 15-30 parts of the collagen mixture described in step S1, 10-20 parts of the plant extract described in step S2, 2-4 parts of the vitamin mixture described in step S3, 1-3 parts of D-ribose and 3-7 parts of glucosamine are mixed, stirred and reacted at a temperature of 60-80° C. for 20-40 minutes, and then ultrasonically treated for 15-25 minutes to obtain a collagen-containing composition.

7. The method for preparing a composition containing collagen according to claim 6, characterized in that: The fish scale collagen preparation process of step S1 is as follows: by weight, 10-20 parts of washed fish scales are immersed in 60-100 parts of 0.3-0.6 mol / L sodium hydroxide solution for 2-4 hours, taken out and immersed in 50-90 parts of 0.5-1 mol / L hydrochloric acid solution for 1-2 hours, and washed until neutral; then 80-120 parts of deionized water and 2-4 parts of collagenase are added, and the reaction is carried out at a temperature of 100-120° C. for 30-50 minutes; the filtrate obtained by filtration is treated with a nanofilter and an ion exchange resin, and dried at 80-100° C. to obtain fish scale collagen.

8. The method for preparing a collagen-containing composition according to claim 5, characterized in that: The deer collagen preparation process of step S1 is as follows: based on weight, 10-20 parts of washed deer bones are immersed in 40-80 parts of 0.8-1.3 mol / L sodium hydroxide solution for 1-2 hours, taken out and immersed in 60-80 parts of 1.5-2.5 mol / L hydrochloric acid solution for 1-2 hours, and washed until neutral; Subsequently, 70-100 parts of deionized water and 1-3 parts of rennet are added, and the reaction is carried out at a temperature of 100-120° C. for 45-75 minutes; the filtrate obtained by filtration is treated with a nano filter and an ion exchange resin, and dried at 80-100° C. to obtain deer collagen.

9. The method for preparing a composition containing collagen according to claim 5, characterized in that: The bovine collagen preparation process of step S1 is as follows: by weight, 15-25 parts of washed bovine bones are immersed in 60-90 parts of 2-4 mol / L sodium hydroxide solution for 1-4 hours, taken out and immersed in 80-100 parts of 2-3 mol / L sulfuric acid solution for 1.5-2.5 hours, and washed until neutral; Subsequently, 75-95 parts of deionized water and 2-4 parts of tissue protease are added, and the mixture is reacted at a temperature of 100-120° C. for 50-70 minutes; the filtrate obtained by filtration is treated with a nano filter and an ion exchange resin, and dried at 80-100° C. to obtain bovine collagen.

10. Use of the collagen-containing composition prepared according to any one of claims 1 to 9 in food preparation.

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