Grape seed essential oil and preparation method and application thereof

Abstract

The present invention discloses a grape seed essence oil and a preparation method and application thereof. The present invention provides a grape seed essence oil, the grape seed essence oil is composed of an oil phase and an aqueous phase, the oil phase includes a base oil, an antioxidant active substance, an oxidation stabilizer, and an emollient; the aqueous phase includes a moisturizer, a preservative, and water; the mass ratio of the oil phase to the aqueous phase is 1: (1.5-4); wherein, in terms of mass percentage, the content of each component is as follows: the content of the base oil is 10%-20%, the content of the antioxidant active substance is 2%-15%, the content of the oxidation stabilizer is 1%-5%, the content of the emollient is 1%-10%, the content of the preservative is 0.1%-1.0%, and the content of the moisturizer is 0.01%-0.5%. The grape seed essence oil of the present invention is prepared by unique raw material selection, precise dosage ratio, and special processing technology, and has excellent stability, use feeling, and biological activity.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and more specifically, to grape seed essential oil and a preparation method and application thereof. Background Art

[0002] Water-oil two-phase essence oil is a special cosmetic dosage form, which has both the excellent moisturizing and nourishing properties of essence water and the antioxidant and emollient properties of essence oil. However, existing water-oil two-phase essence oil products have problems such as poor stability and poor feel, which are in urgent need of improvement. Therefore, developing a new method for preparing water-oil two-phase essence oil to improve the stability and feel of the product has become an urgent need in the cosmetics and health care products industry. Summary of the invention

[0003] The technical problem to be solved by the present invention is that the water-oil two-phase essential oil products in the prior art have the defects of poor stability and poor use feeling, and provide a grape seed essential oil and a preparation method and application thereof. The grape seed essential oil of the present invention is prepared by unique raw material selection, precise dosage ratio and special processing technology, and has excellent stability, use feeling and biological activity.

[0004] The present invention provides a grape seed essence oil, which consists of an oil phase and a water phase, wherein the oil phase comprises a base oil, an antioxidant active substance, an oxidation stabilizer and an emollient; the water phase comprises a moisturizer, a preservative and water; and the mass ratio of the oil phase to the water phase is 1:(1.5-4);

[0005] Among them, the content of each component, in percentage by mass, is as follows: the content of the base oil is 10%-20%, the content of the antioxidant active is 2%-15%, the content of the oxidation stabilizer is 1%-5%, the content of the emollient is 1%-10%, the content of the preservative is 0.1%-1.0%, the content of the humectant is 0.01%-0.5%, and the balance is supplemented by water.

[0006] In the present invention, preferably, the mass ratio of the oil phase to the water phase is 1:(1.85-3); for example, 2.5:7.5, 3:7 or 3.5:6.5, and another example is 3:7.

[0007] In the present invention, preferably, the base oil is selected from one or more of squalane, jojoba oil, sweet almond oil and caprylic / capric triglyceride.

[0008] In the present invention, preferably, the base oil is used in an amount of 10%-15% by mass, such as 12%.

[0009] In the present invention, preferably, the antioxidant active substance is selected from one or more of grape seed oil, Lithospermum erythrorhizon root extract, Limnanthes bidentata seed oil and rose oil, for example, grape seed oil and Lithospermum erythrorhizon root extract.

[0010] More preferably, the Lithospermum Erythrorhizon root extract is the cosmetic raw material with serial number 08657 in the "Catalogue of Used Cosmetic Raw Materials (2021 Edition)".

[0011] In the present invention, preferably, the content of the antioxidant active substance is 2%-15%, for example 10%, by mass percentage.

[0012] In the present invention, preferably, the oxidative stabilizer is selected from one or more of vitamin E, butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT), for example, vitamin E.

[0013] In the present invention, preferably, the amount of the oxidation stabilizer is 2%-4% by mass, for example 3%.

[0014] In the present invention, preferably, the emollient is selected from one or more of glycerin, silicone oil, trehalose and vaseline, such as silicone oil.

[0015] In the present invention, preferably, the amount of the emollient is 1%-8% by mass, for example 5%.

[0016] In the present invention, preferably, the moisturizing agent is selected from one or more of sodium hyaluronate, glycerol and trehalose, for example, sodium hyaluronate.

[0017] In the present invention, preferably, the amount of the moisturizer is 0.1%-0.3% by mass, for example 0.2%.

[0018] In the present invention, preferably, the preservative is phenoxyethanol.

[0019] In the present invention, preferably, the amount of the preservative is 0.1%-0.8% by mass; for example, 0.5%.

[0020] In the present invention, preferably, the aqueous phase further comprises a thickener.

[0021] Wherein, the thickener is, for example, xanthan gum.

[0022] Preferably, the amount of the thickener is 0.16% by mass.

[0023] In the present invention, preferably, the grape seed essential oil is composed of the following components in percentage by mass: 10%-20% base oil, 2%-15% antioxidant active ingredient, 1%-5% oxidation stabilizer, 1%-10% emollient, 0.1%-1.0% preservative, 0.01%-0.5% humectant and the balance water, wherein the balance refers to making up 100%.

[0024] In a specific embodiment of the present invention, the grape seed essential oil, in terms of mass percentage, is or includes any of the following solutions:

[0025] Option 1:

[0026] 8% squalane, 4% jojoba oil, 9% grape seed oil, 1% comfrey root extract, 3% vitamin E, 5% silicone oil, 0.5% phenoxyethanol, 0.2% sodium hyaluronate and 69.3% water;

[0027] Option 2:

[0028] 6% squalane, 6% jojoba oil, 8% grape seed oil, 2% comfrey root extract, 3% vitamin E, 5% silicone oil, 0.5% phenoxyethanol, 0.2% sodium hyaluronate and 69.3% water;

[0029] Option 3:

[0030] 4% squalane, 8% jojoba oil, 7% grape seed oil, 3% comfrey root extract, 3% vitamin E, 5% silicone oil, 0.5% phenoxyethanol, 0.2% sodium hyaluronate and 69.3% water;

[0031] Option 4:

[0032] 8% caprylic / capric triglyceride, 4% jojoba oil, 9% grape seed oil, 1% comfrey root extract, 3% vitamin E, 5% silicone oil, 0.5% phenoxyethanol, 0.2% sodium hyaluronate, and 69.3% water;

[0033] Option 5:

[0034] 8% sweet almond oil, 4% jojoba oil, 9% grape seed oil, 1% comfrey root extract, 3% vitamin E, 5% silicone oil, 0.5% phenoxyethanol, 0.2% sodium hyaluronate and 69.3% water;

[0035] Option 6:

[0036] 8% squalane, 4% jojoba oil, 9% meadowfoam seed oil, 1% comfrey root extract, 3% vitamin E, 5% silicone oil, 0.5% phenoxyethanol, 0.2% sodium hyaluronate and 69.3% water;

[0037] Option 7:

[0038] 8% squalane, 4% jojoba oil, 9% grape seed oil, 1% comfrey root extract, 3% vitamin E, 5% silicone oil, 0.5% phenoxyethanol, 0.2% trehalose and 69.3% water;

[0039] Option 8:

[0040] 10% squalane, 7% jojoba oil, 9% grape seed oil, 1% comfrey root extract, 3% vitamin E, 5% silicone oil, 0.5% phenoxyethanol, 0.2% sodium hyaluronate and 69.3% water;

[0041] Option Nine:

[0042] 4% squalane, 3% jojoba oil, 9% grape seed oil, 1% comfrey root extract, 3% vitamin E, 5% silicone oil, 0.5% phenoxyethanol, 0.2% sodium hyaluronate and 69.3% water;

[0043] Preferably, the grape seed essential oil, in terms of mass percentage, is or includes the following solutions:

[0044] 6% squalane, 6% jojoba oil, 8% grape seed oil, 2% comfrey root extract, 3% vitamin E, 5% silicone oil, 0.5% phenoxyethanol, 0.2% sodium hyaluronate and 69.3% water.

[0045] In a certain embodiment, the grape seed essential oil of the present invention has a water droplet bursting phenomenon during use.

[0046] In a certain embodiment, the grape seed essential oil of the present invention forms a water-in-oil structure.

[0047] The present invention also provides a method for preparing grape seed essential oil, which comprises the following steps:

[0048] mixing a base oil, an antioxidant active, an oxidation stabilizer, and an emollient to obtain an oil phase;

[0049] mixing a humectant, a preservative and water to obtain an aqueous phase;

[0050] Mixing the oil phase and the water phase; wherein the mass ratio of the oil phase to the water phase is 1:(1.5-4);

[0051] The types and amounts of the base oil, antioxidant active ingredient, oxidation stabilizer, emollient, moisturizer and preservative are the same as those described in the above-mentioned grape seed essential oil.

[0052] The present invention also provides a grape seed essential oil, which is prepared by the above-mentioned method for preparing the grape seed essential oil.

[0053] The present invention also provides a use of the grape seed essence oil as described above as a skin care product in the field of beauty.

[0054] In one embodiment, the grape seed essential oil of the present invention has anti-wrinkle effects.

[0055] In one embodiment, the grape seed essential oil of the present invention has a firming effect.

[0056] On the basis of being in accordance with the common sense in the art, the above-mentioned preferred conditions can be arbitrarily combined to obtain the preferred embodiments of the present invention.

[0057] The reagents and raw materials used in the present invention are commercially available.

[0058] The positive and progressive effects of the present invention are:

[0059] The positive progressive effects of the present invention are: the grape seed essential oil provided by the present invention has good stability, a refreshing skin feel, is easy to absorb, has good moisturizing properties, is simple in process, is easy to achieve industrial production, and the product has anti-wrinkle and firming effects, is suitable for people of all skin types, and can be widely used in cosmetics.

[0060] In the application, the skin care product is in the form of water and oil. In the grape seed essential oil, the oil component has good moisturizing properties, can deeply moisturize the skin, improve skin dryness, dehydration and other problems, and make the skin softer and smoother. The water component is mainly responsible for skin moisturizing and hydrating. This water-oil compatible form can maximize the product's effectiveness. BRIEF DESCRIPTION OF THE DRAWINGS

[0061] Figure 1 This is a photo of the appearance of the grape seed essential oil obtained in Example 2.

[0062] Figure 2 This is the phenomenon of water droplets appearing during the use of the grape seed essential oil obtained in Example 2.

[0063] Figure 3 The anti-wrinkle effect test results of the grape seed essential oil obtained in Example 2 are shown.

[0064] Figure 4 The results of the skin tightening test of the grape seed essential oil obtained in Example 2 are shown in FIG. DETAILED DESCRIPTION

[0065] The present invention is further described below by way of examples, but the present invention is not limited to the scope of the examples. The experimental methods in the following examples without specifying specific conditions are carried out according to conventional methods and conditions, or selected according to the product specifications.

[0066] The raw material information used in the embodiment of the present invention is as follows:

[0067] Table 1

[0068]

[0069] Lithospermum erythrorhizon root extract* indicates lithospermum erythrorhizon root extract, which is the cosmetic raw material with serial number 08657 in the "Catalogue of Used Cosmetic Raw Materials (2021 Edition)".

[0070] The raw material compositions used in Examples 1-3 of the present invention are shown in Table 2 below:

[0071] Table 2:

[0072]

[0073] Example 1

[0074] This embodiment provides a grape seed essential oil, and the preparation method thereof is as follows:

[0075] Weigh 0.2 g of sodium hyaluronate, add 69.3 g of purified water and 0.5 g of phenoxyethanol, and stir at room temperature until completely dissolved to obtain an aqueous phase;

[0076] Weigh 8 g of squalane, 4 g of jojoba oil, 9 g of grape seed oil, 1 g of lithospermum officinale root extract, 3 g of vitamin E, and 5 g of silicone oil, and stir at room temperature until completely dissolved to obtain an oil phase;

[0077] The oil phase and the water phase are respectively filled into bottles according to the prescribed amounts to obtain grape seed essential oil.

[0078] Example 2

[0079] This embodiment provides a grape seed essential oil, and the preparation method thereof is as follows:

[0080] Weigh 0.2 g of sodium hyaluronate, add 69.3 g of purified water and 0.5 g of phenoxyethanol, and stir at room temperature until completely dissolved to obtain an aqueous phase;

[0081] Weigh 4 g of squalane, 8 g of jojoba oil, 8 g of grape seed oil, 2 g of lithospermum officinale root extract, 3 g of vitamin E, and 5 g of silicone oil, and stir at room temperature until completely dissolved to obtain an oil phase;

[0082] The oil phase and the water phase are respectively filled into bottles according to the prescribed amounts to obtain grape seed essential oil.

[0083] The photo of the grape seed essential oil obtained in Example 2 is as follows Figure 1 As shown by Figure 1 It can be seen that grape seed essential oil is divided into two layers: water and oil.

[0084] Example 3

[0085] This embodiment provides a grape seed essential oil, and the preparation method thereof is as follows:

[0086] Weigh 0.2 g of sodium hyaluronate, add 69.3 g of purified water and 0.5 g of phenoxyethanol, and stir at room temperature until completely dissolved to obtain an aqueous phase;

[0087] Weigh 6 g of squalane, 6 g of jojoba oil, 7 g of grape seed oil, 3 g of lithospermum officinale root extract, 3 g of vitamin E, and 5 g of silicone oil, and stir at room temperature until completely dissolved to obtain an oil phase;

[0088] The oil phase and the water phase are respectively filled into bottles according to the prescribed amounts to obtain grape seed essential oil.

[0089] Example 4

[0090] This embodiment provides a grape seed essential oil, which is different from Example 1 only in that "squalane" is replaced by "caprylic / capric triglyceride", and the other conditions and parameters are the same as those in Example 1.

[0091] Example 5

[0092] This embodiment provides a grape seed essential oil, which is different from Example 1 only in that "squalane" is replaced by "sweet almond oil", and the other conditions and parameters are the same as those in Example 1.

[0093] Example 6

[0094] This embodiment provides a grape seed essential oil, which is different from Example 1 only in that "grape seed oil" is replaced by "lilypads seed oil", and the other conditions and parameters are the same as those in Example 1.

[0095] Example 7

[0096] This embodiment provides a grape seed essence oil, which is different from Example 1 only in that the moisturizing agent "sodium hyaluronate" is replaced by "trehalose", and the other conditions and parameters are the same as those in Example 1.

[0097] Example 8

[0098] The present embodiment provides a grape seed essence oil, which is different from that of Example 1 only in that the proportion of squalane is adjusted to 10%, and the proportion of jojoba oil is adjusted to 7%. According to the proportion of squalane and jojoba oil, the proportion of water is adjusted at the same time; after the adjustment, compared with Example 1, the ratio of the oil phase to the water phase is adjusted from 3:7 to 3.5:6.5, and the other conditions and parameters are the same as those of Example 1.

[0099] Example 9

[0100] The present embodiment provides a grape seed essential oil, which is different from that of Example 1 only in that the proportion of squalane is adjusted to 4%, and the proportion of jojoba oil is adjusted to 8%. The proportion of water is adjusted according to the proportion of squalane and jojoba oil; compared with Example 1, the ratio of the oil phase to the water phase is adjusted from 3:7 to 2.5:7.5, and the other conditions and parameters are the same as those of Example 1.

[0101] Effect Example

[0102] 1. Skin feel

[0103] The essential oils obtained in Examples 1-9 were subjected to skin feel tests.

[0104] (1) Test method

[0105] The skin feel test method uses a questionnaire to randomly select subjects, and the requirements for the subjects are as follows:

[0106] Number of people: 30;

[0107] Age: Women aged 18-65 (excluding pregnant or breastfeeding women);

[0108] Other requirements: Good health, no active allergic diseases, no history of photosensitive skin diseases, no recent use of drugs that may affect the test results, such as antihistamines, immunosuppressants, hormone drugs, etc.;

[0109] Scoring criteria: After the subjects used the essential oils obtained in Examples 1-9, they scored. Before using the essential oils, the water layer and the oil layer of the essential oils need to be shaken evenly. The scores are divided into 5 levels, 0 represents completely unacceptable, 1 represents barely acceptable, 2 represents average, 3 represents like, and 4 represents very like. The scores of 30 subjects were counted and the average value was calculated for summary.

[0110] The picture of the water droplet phenomenon that occurs during the use of the grape seed essence oil obtained in Example 2 is as follows Figure 2 As shown, when the essence oils of Example 1 and Example 3 are used, the phenomenon of water droplets also appears, which indicates that the essence oil of the present invention can effectively increase the moisture content of the skin and continuously moisturize it for a long time.

[0111] (2) Test results

[0112] The skin feel rating results are shown in Table 3:

[0113] Table 3

[0114]

[0115] As shown in Table 3, the grape seed essential oil obtained in Example 4 has a stronger oily smell than the grape seed essential oils obtained in Examples 1, 2 and 3, and the skin feel changes from being light and easy to absorb to being greasy.

[0116] The grape seed essence oil obtained in Example 5 has better moisturizing effect than the grape seed essence oil obtained in Examples 1, 2 and 3, but has a greasy feel on the skin. After use, the skin feels slightly greasy and has poor air permeability.

[0117] Compared with the grape seed essential oils obtained in Examples 1, 2 and 3, the grape seed essential oil obtained in Example 6 is absorbed more slowly after the grape seed oil is replaced with meadowfoam seed oil, and the skin feels slightly thick, and the breathability and moisturizing effects are poor.

[0118] Compared with the grape seed essence oil obtained in Examples 1, 2 and 3, the grape seed essence oil obtained in Example 7 has lost its "smooth feeling" and has a poor skin feel after sodium hyaluronate is changed to trehalose.

[0119] Compared with the grape seed essence oils obtained in Examples 1, 2, and 3, the grape seed essence oil obtained in Example 8 has a higher oil phase ratio, is absorbed more slowly, and feels slightly greasy on the skin.

[0120] Compared with the grape seed essence oils obtained in Examples 1, 2, and 3, the grape seed essence oil obtained in Example 9 has a lower oil phase ratio, but the skin feels less moisturizing and has poor moisturizing properties.

[0121] 2. Routine 9 tests

[0122] The grape seed essential oil obtained in Example 2 was subjected to conventional 9 tests.

[0123] (1) Test method

[0124] The total colony count, total mold and yeast count, heat-resistant coliform group, Staphylococcus aureus and Pseudomonas aeruginosa were determined in accordance with the microbial testing methods in Chapter 5 of the "Technical Specifications for Safety of Cosmetics" (2015 edition).

[0125] According to Chapter 4, 1.6 of the "Technical Specifications for Safety of Cosmetics", the heavy metals of lead, arsenic, mercury and cadmium were determined using inductively coupled plasma mass spectrometry;

[0126] Dioxane was determined according to the second method of gas chromatography-mass spectrometry in Chapter 4, 2.19 of the "Technical Specifications for Safety of Cosmetics";

[0127] (2) Test results

[0128] The results of microbiological tests are shown in Table 4:

[0129] Table 4

[0130]

[0131] As can be seen from Table 4, the total colony count, total mold and yeast count, thermotolerant coliform group, Staphylococcus aureus and Pseudomonas aeruginosa all meet the requirements of the "Technical Specifications for Safety of Cosmetics" (2015 edition).

[0132] The test results of heavy metals lead, arsenic, mercury, cadmium and dioxane are shown in Table 5:

[0133] Table 5:

[0134]

[0135] It can be seen from Table 5 that the contents of lead, arsenic, mercury, cadmium and dioxane all meet the requirements of the "Technical Specifications for Safety of Cosmetics" (2015 edition).

[0136] 3. Anti-wrinkle efficacy testing

[0137] The anti-wrinkle efficacy of the grape seed essential oil obtained in Example 2 was tested.

[0138] (1) Test method

[0139] The anti-wrinkle effect is tested using a free radical scavenging experimental method.

[0140] Detection principle: 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH for short) is a stable long-lived free radical. Its ethanol solution is dark purple and has strong absorption near 517nm. When there is a free radical scavenger, the light absorption of DPPH ethanol solution is weakened due to pairing with its single electron. The degree of fading of DPPH ethanol solution is linearly related to the number of electrons it accepts, which can be used to evaluate the ability of the test sample to scavenge free radicals, that is, the size of the antioxidant activity.

[0141] The test sample is: a 95% ethanol solution of grape seed essential oil with a volume concentration of 20%. Preparation method: Mix the grape seed essential oil obtained in Example 2 with 95% ethanol, wherein the volume ratio of the grape seed essential oil obtained in Example 2 to 95% ethanol is 1:4, to obtain.

[0142] Positive samples are used to verify the accuracy of the method and have no practical significance.

[0143] The free radical scavenging rate was tested as follows:

[0144] 1) Sample processing: the sample was diluted with 95% ethanol to multiple concentrations (sample concentrations were 20%, 50%, and 70%);

[0145] 2) The pretreated sample reacts with the DPPH reagent;

[0146] 3) After the reaction, measure the OD value at 517nm;

[0147] 4) Calculation of results

[0148] Calculate the DPPH free radical scavenging rate: ;

[0149] T-sample tube absorbance, that is, the absorbance of the solution after the sample reacts with DPPH;

[0150] T 0 -Sample background absorbance;

[0151] The average value of the absorbance of the C-DPPH tube three times is the absorbance of the DPPH solution when no sample is added;

[0152] C 0 -Solution background absorbance;

[0153] (2) Results

[0154] The results of the free radical scavenging rate of Example 2 are statistically shown as follows: Figure 3 As shown, compared with the negative sample, when the concentration of the tested sample was 20%, the free radical scavenging rate was about 87.47%, which had a certain scavenging effect, indicating that the experimental sample had a certain anti-wrinkle effect.

[0155] Compared with the grape seed essential oil obtained in Example 2, the free radical scavenging rate of the grape seed essential oil obtained in Example 6 is reduced from 87.47% to 22.35%, indicating that after replacing "grape seed oil" with "lily padauk oil" in Example 6, the anti-wrinkle effect is worse than that in Example 2 of the present invention.

[0156] 4. Firming effect test

[0157] The anti-wrinkle efficacy of the grape seed essential oil obtained in Example 2 was tested.

[0158] (1) Test method

[0159] The firming effect was tested using an elastase activity test method.

[0160] Detection principle: Elastase is a hydrolytic protease that can hydrolyze insoluble elastin; however, excessive decomposition of elastin will accelerate skin aging. Inhibiting the activity of elastase and slowing down the degradation of elastase can delay aging and reduce wrinkles.

[0161] The test sample is: the grape seed essential oil obtained in Example 2 is mixed with 95% ethanol solution at a volume ratio of 1:1, that is, the grape seed essential oil obtained in Example 2 is diluted with 95% ethanol solution to 50% of the original concentration.

[0162] Positive samples are used to verify the accuracy of the method and have no practical significance.

[0163] The detection method of protease inhibition rate is as follows:

[0164] Sample processing: The sample to be tested was transferred to a 15 mL centrifuge tube, shaken and sonicated to serve as the sample mother solution.

[0165] Test steps:

[0166] Add buffer, sample diluents of different concentrations and substrate AAAPAN to a 96-well plate, incubate at 36°C for 30 minutes, add elastase respectively, and measure the absorbance at a wavelength of 405nm after 30 minutes of action. AD is performed in parallel three times each time. The effect of the sample on the activity of elastin is obtained.

[0167] Well A: sample + elastase;

[0168] Well B: sample + Tris-HCl buffer;

[0169] Well C: Elastase Tris-HCl buffer;

[0170] Well D: Tris-HCl buffer;

[0171] Elastase inhibition rate calculation formula:

[0172] Elastase inhibition rate (%) = [(CD)(AB)] / (CD)*100%; wherein A, B, C, and D represent the absorbance values ​​of well A, well B, well C, and well D, respectively.

[0173] (2) Results

[0174] The results of the protease inhibition rate of Example 2 are statistically shown as follows: Figure 4 As shown, compared with the negative sample, when the concentration of the tested sample was 50%, the inhibition rate of elastase was about 16.68%, which had a certain inhibitory effect, indicating that the experimental sample had a certain firming effect.

[0175] 5. Stability test

[0176] The grape seed essential oil obtained in Example 2 was subjected to a stability test to investigate its stability.

[0177] (1) Test method

[0178] 3M influencing factor test: Place the product at 50℃±2℃ and relative humidity 75%RH±5%RH for 3 months and observe whether there are any changes in appearance, aroma, pH value, hygiene indicators, etc.

[0179] 6M accelerated test: Conduct the test for 6 months at 40℃±2℃ and 75%RH±5%RH to examine the changes in the sensory and hygiene indicators of cosmetics.

[0180] 12M long-term test: Conduct a 24-month test at 25℃±2℃ and 60%RH±10%RH to examine changes in appearance, aroma, pH value, and hygiene indicators.

[0181] (2) Test results

[0182] The results of the aroma, pH, and microbial indexes of the grape seed essential oil obtained in Example 2 after the 3M influencing factor experiment, 6M accelerated experiment, and 12M long-term experiment are shown in Table 6:

[0183] Table 6

[0184]

[0185] As shown in Table 6, the grape seed essential oil obtained in Example 2 has good stability in aroma, pH and microbial indicators after 3M influencing factor test, 6M accelerated test and 12M long-term test, which indicates that the grape seed essential oil of the present invention has excellent stability.

Claims

1. A grape seed essential oil, characterized in that: The grape seed essence oil is composed of an oil phase and a water phase, wherein the oil phase includes a base oil, an antioxidant active substance, an oxidation stabilizer and an emollient; the water phase includes a moisturizer, a preservative and water; and the mass ratio of the oil phase to the water phase is 1:(1.5-4); Among them, the content of each component, in percentage by mass, is as follows: the content of the base oil is 10%-20%, the content of the antioxidant active is 2%-15%, the content of the oxidation stabilizer is 1%-5%, the content of the emollient is 1%-10%, the content of the preservative is 0.1%-1.0%, the content of the humectant is 0.01%-0.5%, and the balance is supplemented by water.

2. The grape seed essential oil according to claim 1, characterized in that It meets one or more of the following conditions: (1) The grape seed essential oil has a water droplet bursting phenomenon during use; (2) The grape seed essential oil is an oil-in-water structure; (3) The mass ratio of the oil phase to the water phase is 1:(1.85-3); for example, 2.5:7.5, 3:7 or 3.5:6.5, and another example is 3:

7.

3. The grape seed essential oil according to claim 1, characterized in that It meets one or more of the following conditions: (1) The base oil is selected from one or more of squalane, jojoba oil, sweet almond oil and caprylic / capric triglyceride; (2) The antioxidant active substance is selected from one or more of grape seed oil, lithospermum officinale root extract, meadowfoam seed oil and rose oil, for example, grape seed oil and lithospermum officinale root extract; Preferably, the lithospermum root extract is a cosmetic raw material with serial number 08657 in the "Catalogue of Used Cosmetic Raw Materials (2021 Edition)"; (3) The oxidative stabilizer is selected from one or more of vitamin E, butylated hydroxyanisole and butylated hydroxytoluene, for example, vitamin E; (4) The emollient is selected from one or more of glycerin, silicone oil, trehalose and vaseline, such as silicone oil; (5) The moisturizing agent is selected from one or more of sodium hyaluronate, glycerol and trehalose, for example, sodium hyaluronate; (6) The preservative is phenoxyethanol.

4. The grape seed essential oil according to claim 1, characterized in that It meets one or more of the following conditions: (1) In terms of mass percentage, the base oil is used in an amount of 10%-15%, for example, 12%; (2) The antioxidant active ingredient content is 2%-15% by mass, for example 10%; (3) In terms of mass percentage, the amount of the oxidation stabilizer is 2%-4%, for example 3%; (4) The amount of the emollient is 1%-8% by mass, for example 5%; (5) In terms of mass percentage, the amount of the moisturizer is 0.1%-0.3%, for example 0.2%; (6) In terms of mass percentage, the amount of the preservative is 0.1%-0.8%, for example 0.5%.

5. The grape seed essential oil according to claim 1, characterized in that The aqueous phase also includes a thickener, such as xanthan gum; preferably, the amount of the thickener is 0.16% by mass.

6. The grape seed essential oil according to claim 1, characterized in that The grape seed essential oil is composed of the following components in percentage by mass: 10%-20% base oil, 2%-15% antioxidant active ingredient, 1%-5% oxidation stabilizer, 1%-10% emollient, 0.1%-1.0% preservative, 0.01%-0.5% moisturizer and the balance water, wherein the balance refers to making up 100%.

7. The grape seed essential oil according to claim 1, characterized in that The grape seed essential oil, in terms of mass percentage, is or includes any of the following solutions: Solution 1: 8% squalane, 4% jojoba oil, 9% grape seed oil, 1% comfrey root extract, 3% vitamin E, 5% silicone oil, 0.5% phenoxyethanol, 0.2% sodium hyaluronate and 69.3% water; Option 2: 6% squalane, 6% jojoba oil, 8% grape seed oil, 2% comfrey root extract, 3% vitamin E, 5% silicone oil, 0.5% phenoxyethanol, 0.2% sodium hyaluronate and 69.3% water; Option 3: 4% squalane, 8% jojoba oil, 7% grape seed oil, 3% comfrey root extract, 3% vitamin E, 5% silicone oil, 0.5% phenoxyethanol, 0.2% sodium hyaluronate and 69.3% water; Option 4: 8% caprylic / capric triglyceride, 4% jojoba oil, 9% grape seed oil, 1% comfrey root extract, 3% vitamin E, 5% silicone oil, 0.5% phenoxyethanol, 0.2% sodium hyaluronate, and 69.3% water; Option 5: 8% sweet almond oil, 4% jojoba oil, 9% grape seed oil, 1% comfrey root extract, 3% vitamin E, 5% silicone oil, 0.5% phenoxyethanol, 0.2% sodium hyaluronate and 69.3% water; Option 6: 8% squalane, 4% jojoba oil, 9% meadowfoam seed oil, 1% comfrey root extract, 3% vitamin E, 5% silicone oil, 0.5% phenoxyethanol, 0.2% sodium hyaluronate and 69.3% water; Option 7: 8% squalane, 4% jojoba oil, 9% grape seed oil, 1% comfrey root extract, 3% vitamin E, 5% silicone oil, 0.5% phenoxyethanol, 0.2% trehalose and 69.3% water; Option 8: 10% squalane, 7% jojoba oil, 9% grape seed oil, 1% comfrey root extract, 3% vitamin E, 5% silicone oil, 0.5% phenoxyethanol, 0.2% sodium hyaluronate and 69.3% water; Option Nine: 4% squalane, 3% jojoba oil, 9% grape seed oil, 1% comfrey root extract, 3% vitamin E, 5% silicone oil, 0.5% phenoxyethanol, 0.2% sodium hyaluronate and 69.3% water; Preferably, the grape seed essential oil, in terms of mass percentage, is or includes the following solutions: 6% squalane, 6% jojoba oil, 8% grape seed oil, 2% comfrey root extract, 3% vitamin E, 5% silicone oil, 0.5% phenoxyethanol, 0.2% sodium hyaluronate and 69.3% water.

8. A method for preparing grape seed essential oil, characterized in that: It includes the following steps: mixing a base oil, an antioxidant active, an oxidation stabilizer, and an emollient to obtain an oil phase; mixing a humectant, a preservative and water to obtain an aqueous phase; Mixing the oil phase and the water phase; wherein the mass ratio of the oil phase to the water phase is 1:(1.5-4); The types and amounts of the base oil, antioxidant active ingredient, oxidative stabilizer, emollient, moisturizer and preservative are all as described in the grape seed essential oil according to any one of claims 1-7.

9. A grape seed essential oil, characterized in that: The grape seed essential oil is prepared by the preparation method of the grape seed essential oil as claimed in claim 8.

10. Use of the grape seed essential oil according to any one of claims 1 to 7 or claim 9 as a skin care product in the field of beauty; Preferably, the grape seed essential oil has anti-wrinkle effects; Preferably, the grape seed essential oil has a firming effect.

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