Anti-hair loss and growth composition, preparation method and application thereof

Abstract

The invention discloses a composition with anti-hair loss and hair development efficacy, which is prepared from the following raw materials in weight percentage: 50-60% mulberry leaf essence, 10-20% lotus seed heart essence, 20-30% forsythia essence, 1-10% orientalis leaf essence, 10-20% green tea essence, and 1-10% lactobacillus fermentation liquid. The composition of the present invention effectively exerts a synergistic effect through different raw material action mechanisms, enhances VEGF and LEFI target expression, and achieves anti-hair loss and hair development through the following two approaches: first, regulating capillary growth and local blood circulation, providing nutrition for hair follicle growth; second, promoting the proliferation and differentiation of hair follicle cells, extending the hair follicle growth cycle, inhibiting hair follicle cell apoptosis, stimulating hair follicle growth factor and consolidating new and old hair follicles, thereby achieving the effect of anti-hair loss and hair development.

Description

Technical Field

[0001] The invention belongs to the technical field of daily chemical products, and in particular relates to an anti-hair loss and growth composition, and a preparation method and application thereof. Background Art

[0002] The amount of human hair is determined by the number of hair follicles. A healthy hair follicle can grow 1-3 hairs. Under normal circumstances, the number of hair follicles in a human's life is constant. Humans cannot grow new hair follicles. After the hair follicles shrink and degenerate, they no longer participate in the body's circulation and cannot absorb nutrients from the surrounding scalp. The hair follicles shrink and degenerate, and cannot regenerate, and the hair cannot grow again. Therefore, making the hair follicles grow healthily and restoring their functions has become an important solution to prevent hair loss and promote hair growth. Summary of the invention

[0003] The purpose of the present invention is to overcome the problems existing in the prior art and provide an anti-hair loss and hair growth composition and its preparation method and application. The present invention mainly achieves healthy hair growth by combining plant extraction and fermentation to achieve multi-target and multi-level deep hair care and hair growth.

[0004] The purpose of the present invention and the solution to the technical problem are achieved by adopting the following technical solutions.

[0005] One aspect of the present invention provides an anti-hair loss and hair growth composition, the components of the composition include: mulberry leaf essence, lotus seed essence, forsythia essence, cypress leaf essence, green tea essence and lactobacillus fermentation liquid, and the content of each component raw material is: 50-60% of mulberry leaf essence, 10-20% of lotus seed essence, 20-30% of forsythia essence, 1-10% of cypress leaf essence, 10-20% of green tea essence and 1-10% of lactobacillus fermentation liquid according to weight percentage.

[0006] In some preferred embodiments of the present invention, the preparation method of the mulberry leaf essence is: add purified water 15 times the weight of the raw material to the mulberry leaves, stir for 2.5 hours at 70-80°C, extract twice, let stand for 10 minutes and filter, combine the two filtrates, centrifuge, concentrate to a solid content of 6-8%, add 1.5 times butanediol, centrifuge, and obtain the mulberry leaf essence.

[0007] In some preferred embodiments of the present invention, the preparation method of the lotus seed core essence is: crush the lotus seed core, add purified water 15 times the weight of the raw material, stir for 2.5 hours at 70-80°C, extract twice, let stand for 10 minutes and filter, combine the two filtrates, centrifuge, concentrate to a solid content of 6-8%, add 1.5 times butanediol, and centrifuge to obtain the lotus seed core essence.

[0008] In some preferred embodiments of the present invention, the preparation method of the Forsythia suspensa essence is: crush the Forsythia suspensa, add purified water 15 times the weight of the raw material, stir for 2.5 hours at 70-80°C, extract twice, let stand for 10 minutes and filter, combine the two filtrates, centrifuge, concentrate to a solid content of 6-8%, add 1.5 times butanediol, centrifuge, and obtain the Forsythia suspensa essence.

[0009] In some preferred embodiments of the present invention, the preparation method of the Platycladus orientalis leaf essence is: add purified water 15 times the weight of the raw material to the Platycladus orientalis leaves, stir for 2.5 hours at 70-80°C, extract twice, let stand for 10 minutes and filter, combine the two filtrates, centrifuge, concentrate to a solid content of 6-8%, add 1.5 times butanediol, and centrifuge to obtain the Platycladus orientalis leaf essence.

[0010] In some preferred embodiments of the present invention, the preparation method of the green tea essence is: adding 60% butanediol to the green tea extract to make its solid content 4%, and centrifuging to obtain the green tea essence.

[0011] In some preferred embodiments of the present invention, the preparation method of the lactobacillus fermentation broth is: inoculate lactobacillus seeds into the culture medium at a concentration of 1-10%, culture at 25-37° C. for 24 h-48 h, and then treat at 70-85° C. for 20-40 min.

[0012] In some preferred embodiments of the present invention, the culture medium is composed of: 10 g of peptone, 15 g of yeast powder, 20 g of glucose, 2 g of citric acid monohydrate, 2 g of diammonium hydrogen phosphate, 0.4 g of dipotassium hydrogen phosphate, and 0.3 g of manganese sulfate. 1 L of distilled water is added, the culture medium is sealed, and high-pressure sterilization is performed at 121° C. for 20 min.

[0013] Another aspect of the present invention also provides a method for preparing the aforementioned composition, comprising the following steps:

[0014] Weigh each raw material according to the ratio, mix them evenly, and obtain a mixed solution;

[0015] The obtained mixed solution is centrifuged at 4-10°C and 4000-6000 rpm for 10-30 min, and the obtained supernatant is the composition.

[0016] Another aspect of the present invention also provides a use of the aforementioned composition or the composition prepared by the aforementioned method in the preparation of shampoo and hair care products.

[0017] By means of the above technical solution, the present invention has at least the following advantages:

[0018] The present invention uses a combination of plant essence and lactobacillus fermentation broth to effectively exert a synergistic effect through the action mechanism of different raw materials, enhance the expression of VEGF and LEFI targets, prolong the hair follicle growth cycle, stimulate hair follicle growth factor and consolidate new and old hair follicles, restore the function of hair follicles, and achieve the function of preventing hair loss and promoting hair growth.

[0019] The plant raw materials used in the present invention include mulberry leaves, lotus seed core, forsythia, arborvitae leaves, and green tea. Mulberry leaves, as a traditional Chinese herbal medicine, are rich in flavonoids, vitamins, minerals, polyphenols and other functional ingredients, which can inhibit the activity of 5a-reductase, reduce the generation of DHT, reduce hair follicle damage, and provide nutrients for hair follicle growth, thereby promoting hair follicle health. Green tea is rich in tea polyphenols, which has the effects of anti-oxidation and reducing scalp cell damage, while also promoting scalp blood circulation, enhancing hair nutrition supply, inhibiting scalp bacterial growth, reducing scalp inflammation and maintaining scalp health. Lotus seed core is the green embryo in the middle of lotus seeds, which is rich in protein, vitamins, minerals, active ingredients such as lianxin alkaloids, and has the effects of anti-inflammatory, anti-oxidation, and inhibiting sebaceous glands from secreting oil, thereby effectively reducing the damage caused by oil accumulation in hair follicles to the hair follicle structure. Forsythia comes from the dried fruit of the deciduous shrub forsythia of the Oleaceae family, which is rich in flavonoids, polysaccharides, forsythia glycosides and other active ingredients, and can effectively activate the proliferation of hair matrix cells, thereby promoting hair growth. Platycladus orientalis leaves are the branches and leaves of the plant Platycladus orientalis of the Cupressaceae family. They are rich in flavonoids, polyphenols and other effective ingredients, which can promote blood circulation in the scalp, enhance the nutritional supply of hair, and promote hair growth and fixation. The fermentation products of lactobacillus are rich in polysaccharide proteins, which can improve the skin microecology and inhibit bacteria.

[0020] The above description is only an overview of the technical solution of the present invention. In order to more clearly understand the technical means of the present invention and implement it according to the contents of the specification, the preferred embodiments of the present invention are described in detail as follows. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a statistical diagram of the relative expression of VEGF in the anti-hair loss and hair growth compositions of Examples 1-3;

[0022] Figure 2 It is a statistical diagram of the relative expression of VEGF in the anti-hair loss and hair growth compositions of Comparative Examples 1-5;

[0023] Figure 3 It is a statistical graph of the free radical scavenging rate and the relative expression level of LEF1 of the anti-hair loss and hair growth compositions of Examples 1-3 and Comparative Examples 1-5. DETAILED DESCRIPTION

[0024] In order to make the technical means, creative features, objectives and effects of the present invention easy to understand, the technical solutions in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] Unless otherwise specified, the mulberry leaves, lotus seed cores, forsythia and arborvitae leaves mentioned in the present invention are all provided by Anhui Shunhetang Chinese Medicine Pieces Company; butanediol is cosmetic grade and is provided by Guangzhou Ruoyuan Chemical Technology Co., Ltd.; green tea extract is provided by Xi'an Luoshen Biotechnology Co., Ltd., with a polyphenol content of 80%-100%.

[0026] Unless otherwise specified, the free radical scavenging rate in the present invention refers to the Shanghai Daily Chemical Industry Association Group Standard T / SHRH006-2018.

[0027] The relative expression of VEGF and LEF1 refers to QPCR, and the specific operation process is as follows:

[0028] Experimental principle:

[0029] Realtime fluorescence quantitative PCR (RTFQ PCR) uses fluorescent dyes or specific fluorescent-labeled probes to label and track PCR products, monitor the reaction process in real time online, and can analyze the products and calculate the initial concentration of the sample template in combination with the corresponding software.

[0030] Equipment and Materials:

[0031] QPCR instrument, cell culture incubator, centrifuge, clean bench

[0032] Culture medium, trypsin, serum, RNA extraction kit, reverse transcription kit, fluorescent dye

[0033] Detection method:

[0034] 1. Extract nucleic acid: Extract target DNA or RNA.

[0035] 2. Reverse transcription (RNA sample): RNA is transcribed into corresponding cDNA.

[0036] 3. Pretreatment: Purify the extracted DNA / cDNA to remove contaminants.

[0037] 4. Design primers and probes: Design specific primers and fluorescent probes to specifically bind to the target sequence.

[0038] 5. Prepare the reaction system: prepare primers, probes, templates, polymerase, buffer, etc. according to the instructions.

[0039] 6. Perform PCR cycles: Load the reaction system into a thermal cycler and perform PCR cycles to detect DNA denaturation, primer binding, amplification, and fluorescent signals.

[0040] 7. Data analysis: According to the changes in fluorescence signals, analyze the Ct value and calculate the relative quantity of the target sequence.

[0041] Unless otherwise specified, the preparation method of the mulberry leaf essence in the embodiment of the present invention is as follows: crush the mulberry leaves, then add purified water 15 times the weight of the raw material, stir for 2.5 hours at 75°C, extract twice, let stand for 10 minutes and filter, combine the two filtrates, centrifuge at 10°C and 6000rpm for 30 minutes, concentrate on a rotary evaporator at 60°C to a solid content of 7.5%, add 1.5 times butanediol, and centrifuge at 10°C and 6000rpm for 30 minutes to obtain the mulberry leaf essence.

[0042] Unless otherwise specified, the preparation method of the mulberry leaf essence in the embodiment of the present invention is as follows: crush the mulberry leaves, then add purified water 15 times the weight of the raw material, stir for 2.5 hours at 75°C, extract twice, let stand for 10 minutes and filter, combine the two filtrates, centrifuge at 10°C and 6000rpm for 30 minutes, concentrate on a rotary evaporator at 60°C to a solid content of 7.5%, add 1.5 times butanediol, and centrifuge at 10°C and 6000rpm for 30 minutes to obtain the mulberry leaf essence.

[0043] Unless otherwise specified, the preparation method of the Platycladus orientalis leaf essence in the embodiment of the present invention is: crush the Platycladus orientalis leaves, then add purified water 15 times the weight of the raw material, stir for 2.5 hours at 75°C, extract twice, let stand for 10 minutes and filter, combine the two filtrates, centrifuge at 10°C and 6000rpm for 30 minutes, concentrate on a rotary evaporator at 60°C, concentrate to a solid content of 7.5%, add 1.5 times butanediol, and centrifuge at 10°C and 6000rpm for 30 minutes to obtain the Platycladus orientalis leaf essence.

[0044] Unless otherwise specified, the preparation method of the Forsythia suspensa essence in the embodiment of the present invention is as follows: crush the Platycladus orientalis leaves, then add purified water 15 times the weight of the raw material, stir for 2.5 hours at 75°C, extract twice, let stand for 10 minutes and filter, combine the two filtrates, centrifuge at 10°C and 6000rpm for 30 minutes, concentrate on a rotary evaporator at 60°C, concentrate to a solid content of 7.5%, add 1.5 times butanediol, and centrifuge at 10°C and 6000rpm for 30 minutes to obtain the Forsythia suspensa essence.

[0045] Unless otherwise specified, the preparation method of the Forsythia suspensa essence in the embodiment of the present invention is: crush the lotus seed core, then add purified water 15 times the weight of the raw material, stir for 2.5 hours at 75°C, extract twice, let it stand for 10 minutes and filter, combine the two filtrates, centrifuge at 10°C and 6000rpm for 30 minutes, concentrate on a rotary evaporator at 60°C, concentrate to a solid content of 7.5%, add 1.5 times butanediol, and centrifuge at 10°C and 6000rpm for 30 minutes to obtain the lotus seed core essence.

[0046] If there is no special explanation, the preparation method of the green tea essence in the embodiment of the present invention is: adding 60% butanediol to the green tea extract to make its solid content 4%, and centrifuging to obtain the green tea essence.

[0047] If no special instructions, the preparation method of lactobacillus fermentation liquid in the embodiment of the present invention is: lactobacillus seed is inoculated into the above-mentioned culture medium at 5% concentration, after culturing for 36h at 35 ℃, 80 ℃ is processed for 30min. The above-mentioned culture medium consists of: peptone 10g, yeast powder 15g, glucose 20g, citric acid monohydrate 2g, diammonium hydrogen phosphate 2g, dipotassium hydrogen phosphate 0.4g, manganese sulfate 0.3g, add 1L distilled water, seal, 121 ℃ high pressure sterilization 20min. Wherein, lactobacillus is plant lactobacillus CGMCCNO.23158 or CGMCC NO.23159, which is preserved in the General Microbiological Center of China Microbiological Culture Collection Administration Committee, and these two bacteria have been submitted to patent applications by applicant Nanjing Shengde Biotechnology Research Institute Co., Ltd.

[0048] Embodiment 1:

[0049] The formula of the anti-hair loss and hair growth composition of this embodiment is: 450 grams of mulberry leaf essence; 80 grams of lotus seed core essence; 220 grams of forsythia essence; 200 grams of green tea essence; 30 grams of Platycladus orientalis leaf essence; and 20 grams of lactobacillus fermentation product.

[0050] The raw materials are mixed uniformly to obtain a mixed solution, and the mixed solution is centrifuged at 10° C. and 6000 rpm for 10 minutes using a high-speed centrifuge to obtain 1000 g of an anti-hair loss and growth composition.

[0051] Embodiment 2:

[0052] The formula of the anti-hair loss and hair growth composition of this embodiment is: 400 grams of mulberry leaf essence; 80 grams of lotus seed core essence; 220 grams of forsythia essence; 250 grams of green tea essence; 30 grams of Platycladus orientalis leaf essence; and 20 grams of lactobacillus fermentation product.

[0053] The raw materials are mixed uniformly to obtain a mixed solution, and the mixed solution is centrifuged at 10° C. and 6000 rpm for 10 minutes using a high-speed centrifuge to obtain 1000 g of an anti-hair loss and growth composition.

[0054] Embodiment 3:

[0055] The formula of the anti-hair loss and hair growth composition of this embodiment is: 400 grams of mulberry leaf essence; 100 grams of lotus seed core essence; 200 grams of forsythia essence; 250 grams of green tea essence; 30 grams of Platycladus orientalis leaf essence; and 20 grams of lactobacillus fermentation product.

[0056] The raw materials are mixed uniformly to obtain a mixed solution, and the mixed solution is centrifuged at 10° C. and 6000 rpm for 10 minutes using a high-speed centrifuge to obtain 1000 g of an anti-hair loss and growth composition.

[0057] Comparative ratio;

[0058] Comparative Example 1: The formula of the anti-hair loss and hair growth composition of this comparative example is: mulberry leaf essence: 400 grams.

[0059] The obtained mulberry leaf essence is centrifuged at 10°C and 6000rpm for 10 minutes using a high-speed centrifuge to obtain the product.

[0060] Comparative Example 2: The formula of the anti-hair loss and hair growth composition of this comparative example is: mulberry leaf essence: 400 grams; lotus seed core essence: 100 grams.

[0061] The above raw materials are mixed evenly to obtain a mixed solution, and the obtained mixed solution is centrifuged in a high-speed centrifuge at 10°C and 6000rpm for 10 minutes to obtain the product.

[0062] Comparative Example 3: The formula of the anti-hair loss and hair growth composition of this comparative example is 400 grams of mulberry leaf essence; 100 grams of lotus seed core essence; and 200 grams of forsythia essence.

[0063] The raw materials were mixed evenly to obtain a mixed solution, and the obtained mixed solution was centrifuged at 10°C and 6000 rpm for 10 minutes using a high-speed centrifuge to obtain the product.

[0064] Comparative Example 4: The formula of the anti-hair loss and hair growth composition of this comparative example is: mulberry leaf essence: 400 grams; lotus seed core essence: 100 grams; forsythia essence: 200 grams; green tea essence: 250 grams.

[0065] The above raw materials are mixed evenly to obtain a mixed solution, and the obtained mixed solution is centrifuged in a high-speed centrifuge at 10°C and 6000rpm for 10 minutes to obtain the product.

[0066] Comparative Example 5: The formula of the anti-hair loss and hair growth composition of this embodiment is: mulberry leaf essence: 400 grams; lotus seed core essence: 100 grams; forsythia suspensa essence: 200 grams; green tea essence: 250; Platycladus orientalis leaf essence: 30 grams.

[0067] The raw materials are mixed uniformly to obtain a mixed solution, and the obtained mixed solution is centrifuged at 10°C and 6000rpm for 10 minutes using a high-speed centrifuge to obtain the product.

[0068] Experiment 1: Detection of free radical scavenging rate and relative expression of VEGF and LEF1 of different compositions

[0069] According to the above method, the free radical scavenging rate and the relative expression of VEGF and LEF1 were tested on the compositions of Examples 1-3 and Comparative Examples 1-5, respectively. The results are shown in Table 1.

[0070] Table 1 Statistics of free radical scavenging rate and relative expression of VEGF and LEF1 in different compositions

[0071] model Free radical scavenging rate% VEGF relative expression% LEF1 relative expression % Example 1 90 3900 250 Example 2 95 3850 245 Example 3 92 3950 240 Comparative Example 1 50 110 100 Comparative Example 2 55 115 120 Comparative Example 3 65 300 140 Comparative Example 4 70 450 160 Comparative Example 5 80 2500 200

[0072] Note: Free radical scavenging rate: detection concentration is 1%, and detection concentration of other indicators is 0.2%.

[0073] It can be seen from the results in Table 1 that compared with comparative examples 1-5, the compositions of Examples 1-3 of the present invention have higher free radical scavenging rates, relative expressions of VEGF and relative expressions of LEF1. The compositions of Examples 1-3 of the present invention effectively exert synergistic effects through different raw material action mechanisms, enhance the expression of VEGF and LEFI targets, and achieve hair loss prevention and hair development through the following two pathways: first, regulating capillary growth and promoting local blood circulation to provide nutrition for hair follicle growth; second, promoting the proliferation and differentiation of hair follicle cells, prolonging the hair follicle growth cycle, inhibiting hair follicle cell apoptosis, stimulating hair follicle growth factors and consolidating new and old hair follicles, thereby achieving hair loss prevention and hair development.

[0074] Experiment 2: Testing of the hair growth and development effects of different compositions

[0075] 1. Preparation of hair growth shampoo

[0076] The composition raw materials of Example 1 and Comparative Examples 1-5 are applied to the shampoo formula to obtain the corresponding hair growth and development shampoo. The specific formula is shown in Table 2:

[0077] Table 2 Hair growth shampoo formula

[0078]

[0079] 2. Preparation of hair growth shampoo

[0080] The hair growth and development shampoo was prepared according to the formula in Table 2, and the specific method was as follows:

[0081] 1. Add the components of phase A into the reactor and stir evenly at room temperature;

[0082] 2. Add phase B components and continue stirring until uniform;

[0083] 3. Add pre-mixed phase C and stir until uniform;

[0084] 4. Add phase D components, stir evenly, and adjust the pH value of the system to 6.0;

[0085] 5. Discharge the material to obtain the hair growth and development shampoo.

[0086] 3. Performance testing of hair growth shampoo

[0087] The shampoos prepared in Example 1 and Comparative Examples 1-5 were tested for their anti-hair loss and hair growth performance. The specific method was as follows: 120 hair loss patients aged 20-50 were selected, each with 20 people in a group, and randomly divided into 6 groups. Different products (anti-hair loss and hair growth shampoos made from the compositions of Example 1 and Comparative Examples 1-5, respectively) were used once every 3 days. The use method was similar to that of ordinary shampoo, and the total use was 3 months. The hair loss and hair growth conditions of the subjects in each group were statistically analyzed, and the results are shown in Table 3 below:

[0088] Table 3 Hair loss and hair growth in each group

[0089] model Number of people whose hair loss condition improved Number of people with hair growth effect Number of people with no effect Hair Growth Shampoo - Example 1 20 15 0 Hair growth shampoo - comparative example 1 10 10 5 Hair growth shampoo - comparative example 2 7 2 13 Hair growth shampoo - comparative example 3 2 2 17 Hair growth shampoo - comparative example 4 2 3 17 Hair growth shampoo - comparative example 5 1 1 18

[0090] It can be seen from the results of Table 3 that, compared with Comparative Examples 1-5, the composition of Example 1 of the present invention is applied to shampoo to improve the hair loss situation more significantly, with the largest number of people with hair growth effect, and the lowest number of people without effect. In addition, by applying the composition of the present invention to the formulas of scalp essence, hair conditioner, etc. for comparison, it is found that the same results are obtained, that is, compared with the composition of Comparative Examples 1-5, Example 1 of the present invention has a higher anti-hair loss and hair growth effect. The compositions of Examples 1-3 of the present invention effectively play a synergistic role through the action mechanism of different raw materials, enhance the expression of VEGF and LEFI targets, and achieve anti-hair loss and hair growth through the following two pathways: first, regulating capillary growth and promoting local blood circulation to provide nutrition for hair follicle growth; second, promoting the proliferation and differentiation of hair follicle cells, prolonging the hair follicle growth cycle, inhibiting hair follicle cell apoptosis, stimulating hair follicle growth factor and consolidating new and old hair follicles, thereby achieving anti-hair loss and hair growth.

[0091] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technician familiar with this profession can make some changes or modifications to equivalent embodiments of equivalent changes by using the methods and technical contents disclosed above without departing from the scope of the technical solution of the present invention. However, any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention without departing from the content of the technical solution of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A hair loss prevention and hair growth composition, characterized in that: The raw materials of the composition include: mulberry leaf essence, lotus seed heart essence, forsythia essence, cypress leaf essence, green tea essence and lactobacillus fermentation liquid. According to weight percentage, the content of each raw material is: 50-60% of mulberry leaf essence, 10-20% of lotus seed heart essence, 20-30% of forsythia essence, 1-10% of cypress leaf essence, 10-20% of green tea essence and 1-10% of lactobacillus fermentation liquid.

2. The composition according to claim 1, characterized in that The preparation method of the mulberry leaf essence is as follows: adding purified water 15 times the weight of the raw material to the mulberry leaves, stirring for 2.5 hours at 70-80° C., extracting twice, standing for 10 minutes and filtering, combining the two filtrates, centrifuging, concentrating to a solid content of 6-8%, adding 1.5 times butanediol, centrifuging, and obtaining the mulberry leaf essence.

3. The composition according to claim 1, characterized in that The preparation method of the lotus seed core essence is as follows: crush the lotus seed core, add purified water 15 times the weight of the raw material, stir for 2.5 hours at 70-80° C., extract twice, let stand for 10 minutes, filter, combine the two filtrates, centrifuge, concentrate to a solid content of 6-8%, add 1.5 times butanediol, centrifuge, and obtain the lotus seed core essence.

4. The composition according to claim 1, characterized in that The preparation method of the forsythia essence is as follows: crush the forsythia, add purified water 15 times the weight of the raw material, stir for 2.5 hours at 70-80°C, extract twice, let stand for 10 minutes and filter, combine the two filtrates, centrifuge, concentrate to a solid content of 6-8%, add 1.5 times butanediol, centrifuge, and obtain the forsythia essence.

5. The composition according to claim 1, characterized in that The preparation method of the Platycladus orientalis leaf essence is as follows: adding purified water 15 times the weight of the raw material to the Platycladus orientalis leaves, stirring for 2.5 hours at 70-80° C., extracting twice, letting stand for 10 minutes and filtering, combining the two filtrates, centrifuging, concentrating to a solid content of 6-8%, adding 1.5 times butanediol, and centrifuging to obtain the Platycladus orientalis leaf essence.

6. The composition according to claim 1, characterized in that The preparation method of the green tea essence is as follows: 60% butanediol is added to the green tea extract to make its solid content 4%, and the green tea essence is obtained by centrifugation.

7. The composition according to claim 1, characterized in that The preparation method of the lactobacillus fermentation liquid is as follows: lactobacillus seeds are inoculated into a culture medium at a concentration of 1-10%, cultured at 25-37° C. for 24 h-48 h, and then treated at 70-85° C. for 20-40 min.

8. The composition according to claim 7, characterized in that The culture medium is composed of: 10 g of peptone, 15 g of yeast powder, 20 g of glucose, 2 g of citric acid monohydrate, 2 g of diammonium hydrogen phosphate, 0.4 g of dipotassium hydrogen phosphate, and 0.3 g of manganese sulfate. 1 L of distilled water is added, the medium is sealed, and high-pressure sterilization is performed at 121° C. for 20 min.

9. A method for preparing the composition according to any one of claims 1 to 8, characterized in that: The following steps are involved: Weigh each raw material according to the ratio, mix them evenly, and obtain a mixed solution; The obtained mixed solution is centrifuged at 4-10°C and 4000-6000 rpm for 10-30 min, and the obtained supernatant is the composition.

10. Use of the composition according to claims 1 to 8 or the composition prepared by the method according to claim 9 in preparing shampoo and hair care products.

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