Composite extract for preventing hair loss and controlling oiliness, preparation method and application thereof

Abstract

The present invention relates to the technical field of raw materials for toiletries, and specifically discloses a composite extract for preventing hair loss and controlling oil, a preparation method thereof, and an application thereof. The preparation method of the composite extract for preventing hair loss and controlling oil comprises the following steps: step 1), putting the composite plant raw material into a solvent, heating and extracting, and obtaining a pre-extract solution; step 2), cooling the pre-extract solution to ≤36°C, and then inoculating the composite bacteria into the pre-extract solution for fermentation to obtain a fermentation solution; step 3), sterilizing and concentrating to obtain a composite extract for preventing hair loss and controlling oil; the composite plant raw material is a compound of ginseng root, Platycladus orientalis, Polygonum multiflorum, Ligustrum lucidum, Morinda officinalis, Eucommia ulmoides, Milk thistle, and Taxus cuspidatus; the composite bacteria is a compound of Jardine Seberlindner's yeast, Phanerochaete chrysosporium, Hansen's yeast with grape juice, and Lactococcus lactis subspecies creamy. The present invention has the advantage of further improving the anti-hair loss effect.

Description

Technical Field

[0001] The invention relates to the field of raw materials for toiletries, and in particular to a composite extract for preventing hair loss and controlling oiliness, a preparation method thereof, and applications thereof. Background Art

[0002] Due to changes in lifestyle, eating habits and stress levels, the number of people suffering from hair loss is increasing and getting younger. Hair loss has become the second largest disease in the world. According to the different pathogenesis and hair loss patterns, hair loss can be divided into alopecia areata, psychogenic hair loss, endocrine disorder hair loss, nutritional hair loss, chemical hair loss, seborrheic alopecia and other types. It is reported that 95% of people with hair loss currently suffer from seborrheic alopecia. Seborrheic alopecia is a hair loss disease determined by genes and affected by androgens, usually accompanied by excessive oil secretion on the scalp. Its pathogenesis is that the metabolites generated by testosterone under the catalysis of 5-α reductase bind to androgen receptors, produce abnormal cytokines to attack hair follicles, lead to miniaturization of hair follicles, and hinder hair growth. Therefore, seborrheic alopecia can be significantly improved by inhibiting the activity of 5-α reductase.

[0003] Existing toiletries are endowed with anti-hair loss function by adding plant extracts with anti-hair loss function. However, the effect of existing anti-hair loss products in inhibiting the activity of 5-α reductase is still relatively general, and it is difficult to achieve a significant anti-hair loss effect and meet the needs of consumers. Therefore, there is still room for improvement. Summary of the invention

[0004] In order to further improve the anti-hair loss effect, the present application provides a composite extract for anti-hair loss and oil control, a preparation method and an application thereof.

[0005] In the first aspect, the present application provides a method for preparing a composite extract for preventing hair loss and controlling oiliness, using the following technical solution: A method for preparing a composite extract for preventing hair loss and controlling oiliness comprises the following steps: Step 1), putting the composite plant material into a solvent, heating and extracting, to obtain a pre-extract solution; Step 2), cooling the pre-extract to ≤36°C, and then inoculating the composite bacteria into the pre-extract for fermentation to obtain a fermentation liquid; Step 3), sterilizing and concentrating to obtain a composite extract for preventing hair loss and controlling oiliness; The composite plant raw material is a combination of ginseng root, Platycladus orientalis leaf, Polygonum multiflorum processed, Ligustrum lucidum fruit, Morinda officinalis, Eucommia ulmoides, Milk thistle and Taxus cuspidata; The composite bacteria is a combination of Lindnerella jeddinseberii, Phanerochaete chrysosporium, Hansenula vitis spores, and Lactococcus lactis subsp. cremoris.

[0006] By adopting the above technical scheme, a composite bacteria prepared by combining ginseng root, Platycladus orientalis leaf, Radix Polygoni Multiflori, Fructus Ligustri Lucidi, Morinda officinalis, Eucommia ulmoides, Silybum marianum, Taxus cuspidatum and compounding with Saccharomyces jadinseiberlindnerii, Phanerochaete chrysosporium, Hansenula viticola and Lactococcus lactis subsp. cremoris is fermented, and the composite extract obtained by synergistically cooperating with various active ingredients and fermentation products can significantly inhibit 5α-reductase, have a good inhibitory effect on Malassezia furfur, have a good scavenging effect on free radicals, and can well inhibit the secretion of skin oil, thereby effectively improving the phenomenon of hair loss, and having a very significant anti-hair loss effect, and the composite extract has low irritation, effectively repairs the scalp while reducing the loss of irritation to the skin, making the effect of repairing the scalp more significant, and the composite extract also has good stability, is not prone to discoloration, stratification and the like, the product efficacy can be maintained for a long time, and the quality of the washing products made is more stable.

[0007] Preferably, in step 1), the solvent is an ethanol aqueous solution.

[0008] By adopting the above technical scheme and specifically selecting the ethanol-water solution, the extraction effect is better, the active ingredients are easier to extract, and the obtained composite extract has a better anti-hair loss effect.

[0009] Preferably, the mass concentration of ethanol in the ethanol aqueous solution is 30-40%.

[0010] By adopting the above technical solution and specifically selecting the mass concentration of ethanol to be 30-40%, the extraction effect is further improved, the extraction efficiency is higher, and the product quality is better.

[0011] Preferably, in the composite plant raw material, the mass ratio of ginseng root, Platycladus orientalis leaf, Polygonum multiflorum processed, Fructus Ligustri Lucidi, Morinda officinalis, Eucommia ulmoides, Silybum marianum and Taxus cuspidata is 3.5-4.5:4.5-5.5:3.5-4.5:2.5-3.5:0.5-1.5:2.5-3.5:0.5-1.5:1.5-2.5.

[0012] By adopting the above technical scheme and specifically selecting the mass ratios of ginseng root, Platycladus orientalis leaf, Polygonum multiflorum processed, Fructus Ligustri Lucidi, Morinda officinalis, Eucommia ulmoides, Silybum marianum, and Taxus cuspidata, various Chinese medicinal materials are combined in specific ratios, so that various active ingredients can better exert synergistic effects and the anti-hair loss effect is better.

[0013] Preferably, the mass ratio of Saccharomyces jadiniiberrindisi, Phanerochaete chrysosporium, Hansenula vitis spores, and Lactococcus lactis subsp. cremoris in the composite bacteria is 0.05-0.07: 0.07-0.09: 0.03-0.05: 0.09-0.11.

[0014] By adopting the above technical scheme and specifically selecting the mass ratio of Saccharomyces jadinseberri, Phanerochaete chrysosporium, Hansenula vitis spores, and Lactococcus lactis cremoris, the fermentation product and the active ingredients can cooperate with each other better, thereby better improving the anti-hair loss effect of the composite extract.

[0015] Preferably, in step 1), the mass ratio of the composite plant raw material to the solvent is 1:20-30.

[0016] By adopting the above technical solution, the extraction effect is better, the active ingredients in the composite plant raw materials are easier to precipitate, the extraction efficiency is effectively improved, and the process cost is reduced.

[0017] Preferably, the mass ratio of the composite plant material in step 1) to the composite bacteria in step 2) is 19-27:0.24-0.32.

[0018] By adopting the above technical solution, the fermentation effect is better, and the effect of the fermentation product and the active ingredient in combination is better, which can better improve the anti-hair loss effect of the composite extract.

[0019] Preferably, in the step 3), the mass ratio of the anti-hair loss and oil control composite extract obtained after concentration to the composite plant raw material in the step 1) is 10-12:1.

[0020] By adopting the above technical solution, the concentration of the composite extract is just right, the amount added to the toiletries does not need to be too high, and the anti-hair loss effect is better, which is convenient for transportation and subsequent production steps.

[0021] In the second aspect, the present application provides a composite extract for preventing hair loss and controlling oiliness, which adopts the following technical solution: A composite extract for preventing hair loss and controlling oil is prepared by the preparation method of the composite extract for preventing hair loss and controlling oil.

[0022] By adopting the above technical solution, the prepared composite extract has significant anti-hair loss and oil control effects and can significantly improve the phenomenon of hair loss.

[0023] In the third aspect, the present application provides an application of a composite extract for preventing hair loss and controlling oil, adopting the following technical scheme: an application of a composite extract for preventing hair loss and controlling oil, wherein the composite extract for preventing hair loss and controlling oil is prepared by the preparation method of the composite extract for preventing hair loss and controlling oil and is added to toiletries to prepare toiletries with anti-hair loss and oil control effects, wherein the mass content of the composite extract for preventing hair loss and controlling oil in the toiletries is 1-10%.

[0024] By adopting the above technical solution, the anti-hair loss and oil control composite extract is added to the toiletries, which can give the toiletries significant anti-hair loss and oil control effects and can achieve a good effect of alleviating hair loss.

[0025] In summary, this application has the following beneficial effects: 1. The present invention is a composite extract obtained by fermenting a composite bacteria prepared by combining ginseng root, Platycladus orientalis leaf, Radix Polygoni Multiflori, Fructus Ligustri Lucidi, Morinda officinalis, Eucommia ulmoides, Silybum marianum, Taxus cuspidata and compounding with Saccharomyces cerevisiae, Phanerochaete chrysosporium, Hansenula viticola and Lactococcus lactis subsp. cremoris. The composite extract can significantly inhibit 5α-reductase, has a good inhibitory effect on Malassezia furfur, has a good scavenging effect on free radicals, and can well inhibit the secretion of skin oil, thereby effectively improving the phenomenon of hair loss, and has a very significant anti-hair loss effect. In addition, the composite extract has low irritation, effectively repairs the scalp while reducing the loss of irritation to the skin, making the effect of repairing the scalp more significant. The composite extract also has good stability, is not prone to discoloration, stratification and the like, and the product efficacy can be maintained for a long time, and the quality of the washing products made is more stable.

[0026] 2. In the present application, it is preferred to specifically select the mass ratio of ginseng root, Platycladus orientalis leaf, Polygonum multiflorum processed, Fructus Ligustri Lucidi, Morinda officinalis, Eucommia ulmoides, Silybum marianum, and Taxus cuspidata, so that various Chinese medicinal materials are combined in a specific ratio, so that various active ingredients can better exert synergistic effects and the anti-hair loss effect is better.

[0027] 3. In the present application, it is preferred to specifically select the mass ratio of Saccharomyces jadinseberlindnerii, Phanerochaete chrysosporium, Hansenula vitis spores, and Lactococcus lactis subsp. cremoris, so that the fermentation product and the active ingredients cooperate with each other better, thereby better improving the anti-hair loss effect of the composite extract. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a photo of the anti-hair loss and oil control composite extract of Example 1 after being left for 45 days; Figure 2 This is a photo of the anti-hair loss and oil control composite extract of Example 2 after being left for 45 days; Figure 3 This is a photo of the anti-hair loss and oil control composite extract of Example 3 after being left for 45 days; Figure 4 This is a photo of the anti-hair loss and oil control composite extract of Comparative Example 1 after being left for 45 days; Figure 5 This is a photo of the anti-hair loss and oil control composite extract of Comparative Example 2 after being left for 45 days; Figure 6 This is a photo of the anti-hair loss and oil control composite extract of Comparative Example 3 after being left for 45 days; Figure 7This is a photo of the anti-hair loss and oil control composite extract of Comparative Example 4 after being left for 45 days; Figure 8 This is a photo of the anti-hair loss and oil control composite extract of Comparative Example 5 after being left for 45 days; Fig. 9 This is a photo of the anti-hair loss and oil control composite extract of Comparative Example 6 after being left for 45 days; Fig.10 This is a photo of the anti-hair loss and oil control composite extract of Comparative Example 7 after being left for 45 days; Fig.11 This is a photo of the anti-hair loss and oil control composite extract of Comparative Example 8 after being left for 45 days; Fig.12 This is a photo of the anti-hair loss and oil control composite extract of Comparative Example 9 after being left for 45 days; Fig.13 This is a photo of the anti-hair loss and oil control composite extract of Comparative Example 10 after being left for 45 days; Fig.14 This is a photo of the anti-hair loss and oil control composite extract of Comparative Example 11 after being left for 45 days; Fig.15 This is a photo of the anti-hair loss and oil control composite extract of Comparative Example 12 after being left for 45 days; Fig.16 This is a photo of the anti-hair loss and oil control composite extract of Comparative Example 13 after being left for 45 days; Fig.17 This is a photo of the anti-hair loss and oil control composite extract of Comparative Example 14 after being left for 45 days. DETAILED DESCRIPTION

[0029] The present application is further described in detail below with reference to the embodiments.

[0030] Example 1 A composite extract for preventing hair loss and controlling oiliness, and a preparation method of the composite extract for preventing hair loss and controlling oiliness comprises the following steps: Step 1), 3.5kg of ginseng root, 4.5kg of Platycladus orientalis leaf, 3.5kg of Polygonum multiflorum processed, 2.5kg of Ligustrum lucidum fruit, 0.5kg of Morinda officinalis, 2.5kg of Eucommia ulmoides, 0.5kg of Silybum marianum, 1.5kg of Taxus cuspidata, and 380kg of 30% ethanol aqueous solution are put into a stirred tank, the speed is 60r / min, stirring is continued, heating to boiling, condensing and reflux, extracting for 60min, and obtaining a pre-extracted solution.

[0031] Step 2), the pre-extract solution is cooled to 36° C., and then 0.05 kg of Jedinseiberlindnerella yeast, 0.07 kg of Phanerochaete chrysosporium, 0.03 kg of Hansenula vitis spores, and 0.09 kg of Lactococcus lactis subsp. cremoris are inoculated into the pre-extract solution, and the fermentation is carried out at a constant temperature of 32° C. for 7 days to obtain a fermentation liquid.

[0032] Step 3), the fermentation liquid is filtered through a 200-mesh filter cloth to remove the filter residue, the filtrate is taken, and then the filtrate is sterilized by filtering with a 0.22 μm filter membrane to obtain a sterilized liquid, and the sterilized liquid is concentrated by rotary evaporation at a concentration pressure of 0.01 MPa and a concentration temperature of 50° C. to obtain 170 kg of a composite extract for preventing oil loss and controlling oil.

[0033] The ginseng roots were purchased from Renhe Chinese Herbal Medicine Wholesale Company in Bozhou City and are sun-dried Chinese herbal medicines.

[0034] The Platycladus orientalis leaves were purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd. and sun-dried as traditional Chinese medicinal materials.

[0035] The processed Polygonum multiflorum was purchased from Huanju Medicinal Materials Store in Juancheng County and is a sun-dried Chinese medicinal material.

[0036] Fructus Ligustri Lucidi was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd. and is a sun-dried Chinese medicinal material.

[0037] Morinda officinalis was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd. and is a sun-dried Chinese medicinal material.

[0038] Eucommia ulmoides was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd. and is a sun-dried Chinese medicinal material.

[0039] Milk thistle was purchased from the Zhenpinyuan Chinese herbal medicine store and is a sun-dried Chinese herbal medicine.

[0040] The Northeast Yew was purchased from Wenxiutang Agricultural and Sideline Products Store in Bozhou City and used as a sun-dried Chinese medicinal material.

[0041] Yeast of Jardine Seiberlin was purchased from Beijing Bio-Bowei Biotechnology Co., Ltd., platform number: Bio-57975.

[0042] Phanerochaete chrysosporium was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-83010.

[0043] Hansenula vitis var. vitis vinifera was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-64533.

[0044] Lactococcus lactis subsp. cremoris was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-58672.

[0045] Example 2 A composite extract for preventing hair loss and controlling oiliness, and a preparation method of the composite extract for preventing hair loss and controlling oiliness comprises the following steps: Step 1), 4kg of ginseng root, 5kg of Platycladus orientalis leaf, 4kg of processed Polygonum multiflorum, 3kg of Ligustrum lucidum fruit, 1kg of Morinda officinalis, 3kg of Eucommia ulmoides, 1kg of Silybum marianum, 2kg of Taxus cuspidata, and 575kg of 35% ethanol aqueous solution are put into a stirred tank, the speed is 60r / min, stirring is continued, heating to boiling, condensing and refluxing, extracting for 75min, and obtaining a pre-extracted solution.

[0046] Step 2), the pre-extract solution is cooled to 36° C., and then 0.06 kg of Jedinseiberlindnerella yeast, 0.08 kg of Phanerochaete chrysosporium, 0.04 kg of Hansenula vitis spores, and 0.1 kg of Lactococcus lactis subsp. cremoris are inoculated into the pre-extract solution, and the fermentation is carried out at a constant temperature of 34° C. for 8 days to obtain a fermentation liquid.

[0047] Step 3), the fermentation liquid is filtered through a 200-mesh filter cloth to remove the filter residue, the filtrate is taken, and then the filtrate is sterilized by filtering with a 0.22 μm filter membrane to obtain a sterilized liquid, and the sterilized liquid is concentrated by rotary evaporation at a concentration pressure of 0.01 MPa and a concentration temperature of 50° C. to obtain 253 kg of a composite extract for preventing oil loss and controlling oil.

[0048] The ginseng roots were purchased from Renhe Chinese Herbal Medicine Wholesale Company in Bozhou City and are sun-dried Chinese herbal medicines.

[0049] The Platycladus orientalis leaves were purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd. and sun-dried as traditional Chinese medicinal materials.

[0050] The processed Polygonum multiflorum was purchased from Huanju Medicinal Materials Store in Juancheng County and is a sun-dried Chinese medicinal material.

[0051] Fructus Ligustri Lucidi was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd. and is a sun-dried Chinese medicinal material.

[0052] Morinda officinalis was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd. and is a sun-dried Chinese medicinal material.

[0053] Eucommia ulmoides was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd. and is a sun-dried Chinese medicinal material.

[0054] Milk thistle was purchased from the Zhenpinyuan Chinese herbal medicine store and is a sun-dried Chinese herbal medicine.

[0055] The Northeast Yew was purchased from Wenxiutang Agricultural and Sideline Products Store in Bozhou City and used as a sun-dried Chinese medicinal material.

[0056] Yeast of Jardine Seiberlin was purchased from Beijing Bio-Bowei Biotechnology Co., Ltd., platform number: Bio-57975.

[0057] Phanerochaete chrysosporium was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-83010.

[0058] Hansenula vitis var. vitis vinifera was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-64533.

[0059] Lactococcus lactis subsp. cremoris was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-58672.

[0060] Example 3 A composite extract for preventing hair loss and controlling oiliness, and a preparation method of the composite extract for preventing hair loss and controlling oiliness comprises the following steps: Step 1), 4.5kg of ginseng root, 5.5kg of Platycladus orientalis leaf, 4.5kg of Polygonum multiflorum processed, 3.5kg of Ligustrum lucidum fruit, 1.5kg of Morinda officinalis, 3.5kg of Eucommia ulmoides, 1.5kg of Silybum marianum, 2.5kg of Taxus cuspidata, and 810kg of 40% ethanol aqueous solution are put into a stirred tank, the speed is 60r / min, stirring is continued, heating to boiling, condensing and reflux, extracting for 90min, and obtaining a pre-extracted solution.

[0061] Step 2), the pre-extract solution is cooled to 36° C., and then 0.07 kg of Jedinseiberlindnerella yeast, 0.09 kg of Phanerochaete chrysosporium, 0.05 kg of Hansenula vitis spores, and 0.11 kg of Lactococcus lactis subsp. cremoris are inoculated into the pre-extract solution, and the fermentation is carried out at a constant temperature of 36° C. for 9 days to obtain a fermentation liquid.

[0062] Step 3), the fermentation liquid is filtered through a 200-mesh filter cloth to remove the filter residue, the filtrate is taken, and then the filtrate is sterilized by filtering with a 0.22 μm filter membrane to obtain a sterilized liquid, and the sterilized liquid is concentrated by rotary evaporation at a concentration pressure of 0.01 MPa and a concentration temperature of 50° C. to obtain 324 kg of a composite extract for preventing oil loss and controlling oil.

[0063] The ginseng roots were purchased from Renhe Chinese Herbal Medicine Wholesale Company in Bozhou City and are sun-dried Chinese herbal medicines.

[0064] The Platycladus orientalis leaves were purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd. and sun-dried as traditional Chinese medicinal materials.

[0065] The processed Polygonum multiflorum was purchased from Huanju Medicinal Materials Store in Juancheng County and is a sun-dried Chinese medicinal material.

[0066] Fructus Ligustri Lucidi was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd. and is a sun-dried Chinese medicinal material.

[0067] Morinda officinalis was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd. and is a sun-dried Chinese medicinal material.

[0068] Eucommia ulmoides was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd. and is a sun-dried Chinese medicinal material.

[0069] Milk thistle was purchased from the Zhenpinyuan Chinese herbal medicine store and is a sun-dried Chinese herbal medicine.

[0070] The Northeast Yew was purchased from Wenxiutang Agricultural and Sideline Products Store in Bozhou City and used as a sun-dried Chinese medicinal material.

[0071] Yeast of Jardine Seiberlin was purchased from Beijing Bio-Bowei Biotechnology Co., Ltd., platform number: Bio-57975.

[0072] Phanerochaete chrysosporium was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-83010.

[0073] Hansenula vitis var. vitis vinifera was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-64533.

[0074] Lactococcus lactis subsp. cremoris was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-58672.

[0075] Comparative Example 1 A composite extract for preventing hair loss and controlling oiliness, which is different from Example 2 only in that: Replace the ginseng root with an equal amount of black reishi.

[0076] Black Ganoderma lucidum comes from commercially available, sun-dried Chinese medicinal materials.

[0077] Comparative Example 2 A composite extract for preventing hair loss and controlling oiliness, which is different from Example 2 only in that: Use an equal amount of Astragalus to replace Platycladus orientalis leaves.

[0078] Astragalus comes from commercially available, sun-dried Chinese medicinal materials.

[0079] Comparative Example 3 A composite extract for preventing hair loss and controlling oiliness, which is different from Example 2 only in that: Use an equal amount of ginger root to replace the processed Polygonum multiflorum.

[0080] Ginger root comes from commercially available, sun-dried Chinese medicinal materials.

[0081] Comparative Example 4 A composite extract for preventing hair loss and controlling oiliness, which is different from Example 2 only in that: Use an equal amount of Rhizoma Anemarrhenae to replace Fructus Ligustri Lucidi.

[0082] Rhizoma Anemarrhenae comes from commercially available, sun-dried Chinese medicinal materials.

[0083] Comparative Example 5 A composite extract for preventing hair loss and controlling oiliness, which is different from Example 2 only in that: An equal amount of Galla chinensis was used to replace Morinda officinalis.

[0084] Gallnut comes from commercially available, sun-dried Chinese medicinal materials.

[0085] Comparative Example 6 A composite extract for preventing hair loss and controlling oiliness, which is different from Example 2 only in that: An equal amount of Glycyrrhiza glabra was used to replace Eucommia ulmoides.

[0086] Glycyrrhiza glabra comes from commercially available, sun-dried Chinese medicinal materials.

[0087] Comparative Example 7 A composite extract for preventing hair loss and controlling oiliness, which is different from Example 2 only in that: Replace milk thistle with an equal amount of sanguisorba officinalis.

[0088] Sanguisorba officinalis comes from commercially available, sun-dried Chinese medicinal materials.

[0089] Comparative Example 8 A composite extract for preventing hair loss and controlling oiliness, which is different from Example 2 only in that: An equal amount of the roots of Chuanxiong were used to replace the northeastern yew.

[0090] The root of Chuanxiong comes from commercially available, sun-dried Chinese medicinal materials.

[0091] Comparative Example 9 A composite extract for preventing hair loss and controlling oiliness, which is different from Example 2 only in that: Use an equal amount of black ganoderma to replace ginseng root, use an equal amount of astragalus to replace arborvitae leaf, use an equal amount of ginger root to replace processed Polygonum multiflorum, use an equal amount of Anemarrhena asphodeloides to replace Ligustrum lucidum fruit, use an equal amount of Galla chinensis to replace Morinda officinalis, use an equal amount of Glycyrrhiza uralensis to replace Eucommia ulmoides, use an equal amount of Sanguisorba officinalis to replace Milk thistle, and use an equal amount of Chuanxiong root to replace Taxus cuspidata.

[0092] Black Ganoderma lucidum comes from commercially available, sun-dried Chinese medicinal materials.

[0093] Astragalus comes from commercially available, sun-dried Chinese medicinal materials.

[0094] Ginger root comes from commercially available, sun-dried Chinese medicinal materials.

[0095] Rhizoma Anemarrhenae comes from commercially available, sun-dried Chinese medicinal materials.

[0096] Gallnut comes from commercially available, sun-dried Chinese medicinal materials.

[0097] Glycyrrhiza glabra comes from commercially available, sun-dried Chinese medicinal materials.

[0098] Sanguisorba officinalis comes from commercially available, sun-dried Chinese medicinal materials.

[0099] The root of Chuanxiong comes from commercially available, sun-dried Chinese medicinal materials.

[0100] Comparative Example 10 A composite extract for preventing hair loss and controlling oiliness, which is different from Example 2 only in that: An equal amount of Leuconostoc mesenteroides was used to replace Y. jendingensisberrindnerii.

[0101] Leuconostoc mesenteroides was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-77149.

[0102] Comparative Example 11 A composite extract for preventing hair loss and controlling oiliness, which is different from Example 2 only in that: Kluyveromyces marxianus was used to replace Phanerochaete chrysosporium in equal amounts.

[0103] Kluyveromyces marxianus was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-52525.

[0104] Comparative Example 12 A composite extract for preventing hair loss and controlling oiliness, which is different from Example 2 only in that: Bacillus albopictus was used to replace Hansenula sporogenes in grape juice in equal amounts.

[0105] Bacillus albopictus was purchased from Beijing Biobio Biotechnology Co., Ltd., platform number: Bio-67366.

[0106] Comparative Example 13 A composite extract for preventing hair loss and controlling oiliness, which is different from Example 2 only in that: Lactococcus lactis subsp. cremoris was replaced by Bacillus palmitofermentans in equal amounts.

[0107] Bacillus palmitolyticus was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-79684.

[0108] Comparative Example 14 A composite extract for preventing hair loss and controlling oiliness, which is different from Example 2 only in that: Leuconostoc mesenteroides was used to replace the Jendingseberlindner yeast in equal amounts, Kluyveromyces marxianus was used to replace the Chlamydomonas chrysosporium in equal amounts, Bacillus altaica was used to replace the Hansenula vitis vinifera in equal amounts, and Bacillus palmitofermentans was used to replace the Lactococcus lactis subsp. cremoris in equal amounts.

[0109] Leuconostoc mesenteroides was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-77149.

[0110] Kluyveromyces marxianus was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-52525.

[0111] Bacillus albopictus was purchased from Beijing Biobio Biotechnology Co., Ltd., platform number: Bio-67366.

[0112] Palm fermenting bacillus was purchased from Beijing Bio-win Biotechnology Co., Ltd., platform number: Bio-79684.

[0113] Experiment 1 Detect the 5-α reductase inhibition rate of the anti-hair loss and oil-control composite extracts prepared in each example and comparative example.

[0114] Establish an in vitro model, using testosterone as the substrate, NADPH as the hydrogen donor, and finasteride (0.05 μg / mL) as the positive control group to evaluate the inhibitory effect of the extract on 5α-reductase.

[0115] Experiment 2 Detect the Malassezia furfur inhibition rate of the anti-hair loss and oil-control composite extracts prepared in each example and comparative example.

[0116] Using phosphate buffer as the blank control, and the Malassezia furfur spore suspension + phosphate buffer as the control sample group to evaluate the inhibition rate of the extract on Malassezia furfur. The evaluation index is: when the bacteriostatic rate ≥ 50%, it indicates an anti-dandruff effect, and the higher the inhibition rate, the stronger the anti-dandruff effect.

[0117] Experiment 3 Detect the DPPH free radical scavenging rate of the anti-hair loss and oil-control composite extracts prepared in each example and comparative example.

[0118] Use the in vitro free radical scavenging evaluation method to determine the DPPH free radical scavenging activity of the extract.

[0119] Experiment 4 Detect the intracellular lipid content inhibition rate of the anti-hair loss and oil-control composite extracts prepared in each example and comparative example.

[0120] The experiment uses linoleic acid to induce human sebaceous gland Seb-E6E7 cells with lipid production ability, detects the intracellular lipid content by oil red staining method, measures the change of absorbance of cells in different groups, and calculates the inhibition rate to evaluate the oil-control efficacy of the sample.

[0121] Experiment 5 Detect the irritation of the anti-hair loss and oil-control composite extracts prepared in each example and comparative example.

[0122] Adopt the chicken embryo chorioallantoic membrane blood vessel test, quantify the stimulation according to the reaction of the blood vessels, and evaluate the irritation of the extract to human skin. The evaluation index is: make a score according to the score corresponding to the blood vessel effect, take the average value of a set of data to determine the size of the irritation. When 0 < NC (irritation score) ≤ 2, stress stimulation; 2 < NC ≤ 3, mild stimulation; 3 < NC ≤ 5, moderate stimulation; NC > 5, severe stimulation.

[0123] Experiment 6 The stability of the composite extracts for preventing hair loss and controlling oiliness prepared in the examples and comparative examples was tested.

[0124] Take 50 ml of the anti-hair loss and oil control compound extract and place it in a sealed transparent plastic bottle. Place it under sunlight for 45 days and observe whether there is any discoloration or stratification.

[0125] The specific test data of Experiments 1-6 are shown in Table 1 and Figure 1-Figure 17 .

[0126] Table 1 According to Table 1 and Figure 1-17 From the comparison of experimental data, it can be seen that the 5-α reductase inhibition rate, Malassezia furfur inhibition rate, DPPH free radical scavenging rate, and intracellular lipid content inhibition rate of each embodiment are significantly higher than those of the comparative examples. It can be seen that the anti-hair loss and oil control effects of each embodiment are significantly higher than those of the comparative examples, which proves that only when ginseng root, Platycladus orientalis leaf, Polygonum multiflorum processed, Fructus Ligustri Lucidi, Morinda officinalis, Eucommia ulmoides, Silybum marianum, and Taxus cuspidata are compounded and fermented with Saccharomyces jadinii, Phanerochaete chrysosporium, Hansenula vitis vinifera, and Lactococcus lactis subspecies cremoris can the extract obtained be significantly improved in the anti-hair loss and oil control effect, while the combination of conventional hair care ingredients and the combination of conventional fermented bacteria are difficult to achieve the excellent anti-hair loss and oil control effects of each embodiment.

[0127] Moreover, the irritation of each embodiment is lower than that of each comparative example, which shows that each embodiment is less likely to induce hair loss due to irritation, resulting in a better anti-hair loss effect.

[0128] Finally, the stability of each embodiment is significantly higher than that of each comparative example. No discoloration or stratification occurs after being placed under light for 45 days. Therefore, each embodiment can maintain the anti-hair loss and oil control effects for a longer period of time, and the prepared toiletries still have good anti-hair loss and oil control effects in the later period of the shelf life.

[0129] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A method for preparing a composite extract for preventing hair loss and controlling oiliness, characterized in that: The following steps are involved: Step 1), putting the composite plant raw material into a solvent, heating and extracting, to obtain a pre-extract solution; Step 2), cooling the pre-extract to ≤36°C, and then inoculating the composite bacteria into the pre-extract for fermentation to obtain a fermentation liquid; Step 3), sterilizing and concentrating to obtain a composite extract for preventing hair loss and controlling oiliness; The composite plant raw material is a combination of ginseng root, Platycladus orientalis leaf, Polygonum multiflorum processed, Ligustrum lucidum fruit, Morinda officinalis, Eucommia ulmoides, Milk thistle and Taxus cuspidata; The composite bacteria is a combination of Lindnerella jeddinseberii, Phanerochaete chrysosporium, Hansenula vitis spores, and Lactococcus lactis subsp. cremoris.

2. The method for preparing a composite extract for preventing hair loss and controlling oiliness according to claim 1, characterized in that: In the step 1), the solvent is ethanol aqueous solution.

3. The method for preparing a composite extract for preventing hair loss and controlling oiliness according to claim 2, characterized in that: The mass concentration of ethanol in the ethanol aqueous solution is 30-40%.

4. The method for preparing a composite extract for preventing hair loss and controlling oiliness according to claim 1, characterized in that: In the composite plant raw material, the mass ratio of ginseng root, Platycladus orientalis leaf, Polygonum multiflorum processed, Ligustrum lucidum fruit, Morinda officinalis, Eucommia ulmoides, Silybum marianum and Taxus cuspidata is 3.5-4.5: 4.5-5.5: 3.5-4.5: 2.5-3.5: 0.5-1.5: 2.5-3.5: 0.5-1.5: 1.5-2.

5.

5. The method for preparing a composite extract for preventing hair loss and controlling oiliness according to claim 4, characterized in that: The mass ratio of Saccharomyces jadinseberlindnerii, Phanerochaete chrysosporium, Hansenula vitis spores, and Lactococcus lactis subsp. cremoris in the composite bacteria is 0.05-0.07: 0.07-0.09: 0.03-0.05: 0.09-0.

11.

6. The method for preparing a composite extract for preventing hair loss and controlling oiliness according to claim 5, characterized in that: In the step 1), the mass ratio of the composite plant raw material to the solvent is 1:20-30.

7. The method for preparing a composite extract for preventing hair loss and controlling oiliness according to claim 6, characterized in that: The mass ratio of the composite plant material in step 1) to the composite bacteria in step 2) is 19-27:0.24-0.

32.

8. The method for preparing a composite extract for preventing hair loss and controlling oiliness according to claim 1, characterized in that: In the step 3), the mass ratio of the anti-hair loss and oil control composite extract obtained after concentration to the composite plant raw material in the step 1) is 10-12:

1.

9. A composite extract for preventing hair loss and controlling oiliness, characterized in that: The invention is prepared by the preparation method of the composite extract for preventing hair loss and controlling oiliness according to any one of claims 1 to 8.

10. An application of a composite extract for preventing hair loss and controlling oiliness, characterized in that: The anti-hair loss and oil control composite extract prepared by the preparation method of any one of claims 1-8 is added to toiletries to prepare toiletries with anti-hair loss and oil control effects, wherein the mass content of the anti-hair loss and oil control composite extract in the toiletries is 1-10%.

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