A whitening and firming composition and its application

Abstract

The present invention relates to a whitening and firming composition and its application. The whitening and firming composition comprises bilberry polyphenol, turmeric gel and olive extract, and the mass ratio of the bilberry polyphenol, turmeric gel and olive extract is (0.1-0.825): (0.1-3): (0.1-5). Compared with the prior art, the whitening and firming composition of the present invention makes the skin white and firm for a long time through the scientific selection of functional ingredients, improving skin microcirculation, and multi-target synergy. In addition to the whitening and firming effect, the whitening and firming composition of the present invention has certain antioxidant and repair effects, which can meet people's advanced needs for skin care products. The whitening and firming composition of the present invention has a soothing effect while whitening and firming, and can reduce the irritation of the functional ingredients in cosmetics to the skin.

Description

Technical Field

[0001] The invention belongs to the technical field of daily chemicals, and in particular relates to a whitening and firming composition and application thereof. Background Art

[0002] The main factor that affects skin whiteness is melanin, which exists in the basal layer of everyone's skin. Melanin is a protein derivative produced by melanocytes. It moves through layers of cell metabolism and reaches the epidermis of the skin, thus having a direct impact on skin color. Currently, the whitening effect is generally achieved by reducing the accumulation of melanin in the skin, and the main way is to inhibit melanin production and promote melanin metabolism.

[0003] Tyrosinase is a copper-containing metal enzyme widely found in animals, plants and microorganisms. It is a key enzyme for organisms to synthesize melanin. Therefore, inhibiting the activity or amount of tyrosinase is the key to whitening. Currently, the commonly used tyrosinase inhibitors and their mechanisms of action are as follows: 1. Vitamin C and its derivatives: reduce the reduction of dopaquinone to dopa, thereby reducing the production of dopa pigment and ultimately melanin; 2. Catechol derivatives: reduce the raw materials for the production of melanin by competitively binding to tyrosinase with tyrosine; in addition, there are also raw materials such as SOD, which can block the catalytic reaction of tyrosinase by scavenging oxygen free radicals, thereby weakening the intensity of tyrosine oxidation reaction.

[0004] In addition, skin aging caused by age increase and external environmental factors is an inevitable process. Skin aging manifests itself in dryness and roughness, fine lines and looseness, epidermal thickening, dullness, etc., which seriously affect the appearance of the skin. Among them, sagging skin is one of the most concerned issues for the aging population. Its mechanism involves skin deformation caused by the gradual stretching of the skin by gravity, atrophy of the dermis and subcutaneous fat tissue, damaged elastic fibers and loss of elasticity, and weakening of fat muscle support. Most of the cosmetics on the market currently use the principle of stimulating collagen production and increasing the density, thickness and organization of collagen in the dermis to achieve the purpose of firming. In addition, there are also cosmetics that use botulinum toxin-like ingredients to locally block the nerves that transmit muscle contraction messages, affect the conduction of the skin sac nerves, and relax the facial muscles to achieve an immediate firming effect.

[0005] At present, people's requirements for skin care products are no longer limited to whitening and firming, but they also hope that they have certain antioxidant and repair effects. However, the current skin care products have relatively simple effects. For example, patent CN202210026628.3 discloses a whitening and firming plant essence skin care lotion, including the following raw materials: jojoba oil 2-4%, squalane 1.5-2%, niacinamide 0.35-0.5%, polyglycerol stearate 0.5-1%, tocopherol acetate 1.5-2%, glycerol 10-12%, methylparaben 0.05-0.1%, allantoin 0.2-0.3%, mannan 0.2-0.5%, kenaf leaf extract 3-5%, and the balance is water. Patent CN202211380304.6 discloses a whitening, freckle-removing and anti-wrinkle cream, comprising the following components: Phase A: polydimethylsiloxane; lauryl PEG-10 tri(trimethylsiloxy)silylethyl polydimethylsiloxane; tocopherol; tocopheryl acetate; ascorbyl tetraisopalmitate; hydroxystearic acid; stearic acid; bismuth oxychloride; ethylhexyl hydroxystearate; caprylic / capric triglyceride; colorant; Phase B: water; glycerin; butylene glycol; erythritol; niacinamide; p-hydroxyacetophenone; sodium hyaluronate; sodium polyacrylate; Phase C: 1,2-hexanediol; aluminum starch octenylsuccinate; Phase D: whitening agent; freckle-removing agent; anti-wrinkle agent; emollient; antibacterial agent; fragrance; moisturizer; hydrolyzed pearl; diglucosyl gallic acid.

[0006] However, the whitening product of the above patent has whitening and firming effects but not antioxidant and repairing effects, and cannot meet people's demand for skin care products at the same time;

[0007] Secondly, the whitening products currently on the market mainly use ingredients such as niacinamide, arbutin, and tranexamic acid, which achieve whitening effects by inhibiting the production and transport of melanocytes. However, they have the disadvantages of being highly irritating to the skin and can only act on a single target.

[0008] In addition, most skin care products with firming effects, such as patent CN202010288724.6, disclose a whitening and firming moisturizing cream and its preparation method. The A cream is prepared by dispersing carbomer and arbutin in glycerin to form an aqueous phase, and pre-using stearic acid monoglyceride and stearic acid as the oil phase; hydrolyzed oat protein, pearl hydrolyzate, and acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer are added to deionized water as the aqueous phase; and dicaprate, olive oil, and cetyl polyethylene glycol are used as the oil phase to form B cream; because α-arbutin has poor stability when used in facial cream whitening, it is wrapped in the oil phase with the acidic carbomer, and the weak acid environment of the carbomer allows arbutin to exist in the weak acid environment, thereby stabilizing arbutin in the facial cream. In cream B, hydrolyzed oat protein and pearl hydrolyzate are combined to form oil-in-water. Hydrolyzed oat protein has an instant tightening effect, which can make the skin feel instantly tight. It also has moisturizing, film-forming, and can give the skin a smooth feel. This patent only relaxes the muscles by adding hydrolyzed oat protein and other ingredients, thereby achieving an instant tightening effect. The tightening effect disappears after the product is discontinued, and the skin cannot be tightened for a long time.

[0009] Therefore, the prior art lacks skin care products that simultaneously have whitening, anti-oxidation and skin tightening effects. Summary of the invention

[0010] Based on the lack of skin care products with whitening, anti-oxidation and skin firming effects in the prior art, the present invention provides a whitening and firming composition and application thereof.

[0011] The composition provided in the present application can not only achieve the purpose of whitening and anti-wrinkle by improving microcirculation, but also play a synergistic antioxidant and repair role, making the skin white and firm for a long time.

[0012] The purpose of the present invention can be achieved by the following technical solutions:

[0013] The invention first provides a whitening and firming composition, which comprises bilberry polyphenol, turmeric gel and olive extract, wherein the mass ratio of bilberry polyphenol, turmeric gel and olive extract is (0.1-0.825): (0.1-3): (0.1-5).

[0014] In one embodiment of the present invention, the mass ratio of bilberry polyphenol, turmeric gel and olive extract is (0.1-0.5): (0.1-2): (0.1-3).

[0015] In the present invention, the bilberry polyphenol is an effective component extracted from bilberry (Vaccinium myrtillus Linn.).

[0016] Among them, bilberry (Vaccinium myrtillus Linn.) is usually used as medicine with berries and leaves. The fruit can help the eyes adapt to dim and dark environments, is used to correct myopia, protects peripheral circulation and capillaries, promotes intraocular microcirculation, and improves vision; the leaves have antibacterial effects and improve blood sugar in early diabetic patients. Bilberry contains a large amount of flavonoids, mainly anthocyanins, which help maintain capillary integrity, stabilize collagen, and prevent platelet aggregation and adhesion on the surface of endothelial cells. In the present application, the bilberry polyphenols mainly act as antioxidants, can prevent the formation of red blood streaks by reducing the vasodilation of the skin surface; reduce the permeability of tannic acid, strengthen capillaries; stimulate epidermal blood vessel formation, promote cell information exchange activities, and improve microcirculation.

[0017] The turmeric gel can be purchased.

[0018] Turmeric is the dried rhizome of the ginger plant Curcuma longa L., and is a traditional Chinese medicine. TCM believes that turmeric can promote qi, dispel wind and activate blood circulation, and relieve pain. In recent years, with the rise of Kampo cosmetics, turmeric extracts have been widely used as functional ingredients in cosmetics because of their strong antioxidant, free radical scavenging, anti-inflammatory, ultraviolet absorption, and tyrosinase inhibition capabilities. However, the use of turmeric in the prior art is mainly to utilize its active substances curcumin and turmeric ketone. However, curcumin and turmeric ketone have disadvantages such as volatility and photosensitivity. Direct use in cosmetics is prone to problems such as easy changes in chemical structure and low bioavailability.

[0019] The present application selects turmeric gel to ensure the retention of active substances. Compared with ordinary turmeric extract, the turmeric gel prepared by lactic acid bacteria enrichment fermentation, centrifugal separation, decolorization filtration, concentration and other processes completely retains the volatile component turmeric ketone, and has a light yellow transparent gel texture, which solves the problems of poor water solubility and high volatility, and increases the ease of use of the composition in cosmetic formulations.

[0020] The olive extract in the present invention is specifically an olive leaf extract, which is an active substance extracted from olive (Olea Europaea) leaves using cell wall breaking technology, and contains hundreds of active molecules such as oleuropein and luteolin, and has antioxidant, anti-inflammatory, soothing and redness repairing effects. The olive extract in the present invention specifically uses high-purity bilberry polyphenols and turmeric gel to further ensure the soothing and repairing function of the composition on the skin, reduce the irritation of the effective ingredients to the skin, and can interact synergistically when used at the same time, which is significantly better than the effect of each ingredient used alone. All three can play an antioxidant effect of scavenging free radicals, and can synergistically enhance whitening and firming while repairing.

[0021] The present invention further provides the use of the whitening and firming composition in the preparation of skin care products, and the skin care products can be any form of skin care products or cosmetics, including lotions, creams, freeze-dried powders, skin care water, essences, etc. The skin care products have whitening and firming effects.

[0022] The present invention also provides a skin care product with whitening and firming effects, comprising a matrix auxiliary material and an effective component, wherein the effective component comprises a whitening and firming composition. The auxiliary material is selected from one or a combination of thickeners, skin conditioners, moisturizers, antioxidants, emulsifiers, pH regulators, fragrances or preservatives.

[0023] In some preferred embodiments, based on 100 parts by weight of the total weight of the skin care product, the whitening and firming composition is 1 to 7.8 parts, preferably 3.2 to 7.8 parts.

[0024] In one embodiment of the present invention, the skin care product with whitening and firming effects is a face cream with whitening and firming effects, the face cream comprises an E phase consisting of a whitening and firming composition, and also comprises an A phase auxiliary material, a B phase auxiliary material, a C phase auxiliary material and a D phase auxiliary material;

[0025] Based on 100 parts by weight of the facial cream, the phase A auxiliary material includes 0.2 to 25 parts of thickener A, 0.11 to 11 parts of skin conditioner, and 6 to 30 parts of moisturizer;

[0026] Based on 100 parts by weight of the facial cream, the phase B auxiliary material includes 3 to 25 parts of thickener B, 0.1 to 15 parts of emulsifier, 0.1 to 5 parts of antioxidant and 4 to 20 parts of excipient;

[0027] Based on 100 parts by weight of the facial cream, the phase C auxiliary material includes 0.1 to 5 parts of a pH regulator;

[0028] Based on 100 parts by weight of the facial cream, the phase D auxiliary material includes 0.1 to 5 parts of fragrance and 0.15 to 1 part of preservative;

[0029] Based on 100 parts by weight of the facial cream, the E phase is 1 to 7.8 parts, preferably 3.2 to 7.8 parts.

[0030] Optionally, the phase A auxiliary material also includes water, and the capacity of the water is such that the weight parts of the cream reaches 100 parts.

[0031] In some embodiments of the present invention, the thickener A and the thickener B are independently selected from one or more of acrylic acid (esters) / C10-30 alkyl acrylate crosspolymer, polyacrylamide, xanthan gum, propylene glycol, behenyl alcohol, carbomer, laureth-7, cetyl alcohol, stearyl alcohol or hydroxyethyl cellulose.

[0032] In some embodiments of the present invention, the skin conditioning agent is selected from one or more of isohexadecane, olive oil, sodium hyaluronate, lauryl alcohol, avocado butter, allantoin, squalane, panthenol, lactic acid, tocopherol or dipotassium glycyrrhizinate.

[0033] In some embodiments of the present invention, the moisturizer is selected from one or more of hyaluronic acid, allantoin, glycerin, ceramide, sodium lactate, butylene glycol, hydrogenated grape seed oil, olive oil or shea butter.

[0034] In some embodiments of the present invention, the antioxidant is selected from one or more of tocopherol, tocopherol acetate, grape seed oil, and ascorbic acid.

[0035] In some embodiments of the present invention, the excipient is selected from one or more of squalane, cholesterol, hydrogenated poly (C6-14 olefin), hydrogenated wheat germ oil hydrogenated polyisobutene, avocado tree (BUTYROSPERMUM PARKII) fruit fat, caprylic / capric triglyceride, and polydimethylsiloxane.

[0036] In some embodiments of the present invention, the emulsifier is selected from one or more of glyceryl stearate citrate, cetearyl glucoside (and) cetearyl alcohol, behentrimonium methylsulfate, cocoglycerides or sorbitan olivate.

[0037] In some embodiments of the present invention, the pH adjuster is selected from one or more of arginine or lactic acid.

[0038] In some embodiments of the present invention, the fragrance comprises essence.

[0039] In some embodiments of the present invention, the preservative is selected from one or more of phenoxyethanol, ethylhexylglycerin and pentylene glycol.

[0040] Microcirculation is the flow of blood, lymph and tissue fluid between cells and tissues that participate in substances, energy and biological information. It includes both blood circulation between arterioles and venules, as well as the circulation of lymph and tissue fluid. As the regulatory and functional unit at the end of metabolic regulation, the capillary network system is the basis for achieving microcirculation regulation. The microcirculation of the skin is a complex power system that affects skin nutrition, substance metabolism, immune response, skin color, body temperature regulation and transdermal absorption. Microcirculation plays an important supporting role in various functions of the skin. Under normal circumstances, each cell in the skin obtains nutrients through microcirculation, is activated by various signals, eliminates various wastes, and completes various physiological functions.

[0041] Under abnormal conditions, microcirculatory disorders, skin infections, skin allergies, and skin inflammation will significantly affect microcirculation. At this time, various pathological problems often occur in microcirculation, including increased vascular permeability, leakage of vascular contents into tissue gaps, blood congestion or vasodilation, and local tissue hypoxia to form new capillaries. Microcirculatory disorders can directly lead to cell damage. First, blood congestion and blood cell aggregation occur, the blood flow is sludge-like, tissue cells are damaged by ischemia, cell membrane permeability increases, calcium ion influx increases, mitochondrial oxidative phosphorylation is blocked, ATP is decomposed in large quantities, adenosine hypoxanthine and xanthine metabolites are produced, and a large number of oxygen free radicals are produced through pathways such as xanthine oxidase. The free radicals attack unsaturated fatty acids in the membrane to produce lipid peroxides LPO, which aggravate cell damage and lead to changes in cell membrane structure and function. Improving microcirculation can increase the skin's absorption of oxygen and nutrients, promote the renewal and metabolism of keratinocytes and fibroblasts, and assist the skin in forming more collagen, thereby tightening the skin.

[0042] The present invention improves skin microcirculation and the synergistic effect of multiple functional ingredients to make the skin white and firm for a long time. Improving microcirculation can increase the skin's absorption of oxygen and nutrients, promote the renewal and metabolism of keratinocytes and fibroblasts, and assist the skin in forming more collagen, thereby tightening the skin. The scheme of the present invention particularly adopts a combination of bilberry polyphenols and turmeric gel, wherein bilberry polyphenols contain 12wt% of total polyphenols and 7wt% of total chlorogenic acid; turmeric gel retains the essential oil components in turmeric ketones in the fermentation process, and these essential oil components are usually filtered out during the general fermentation process, resulting in almost no turmeric ketones in the final fermentation product. However, the turmeric gel of the present invention contains 3-5wt% of turmeric oil, of which the content of turmeric ketones is as high as about 50wt%, and this feature greatly retains the active ingredients of turmeric. Furthermore, the present invention adds a multi-functional ingredient olive extract, which not only enhances the soothing and repairing function of the composition on the skin, but also reduces the irritation that the functional ingredients may cause to the skin, and its comprehensive effect is far better than the use of each ingredient alone. The three work synergistically to exert the antioxidant effect of scavenging free radicals, repairing the skin while achieving the comprehensive goal of whitening and firming.

[0043] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0044] The whitening and firming composition of the present invention achieves whitening and firming effects by scientifically selecting effective ingredients, improving skin microcirculation, and synergistic effects of multiple targets, while having anti-oxidation and repair effects, and can meet people's needs for skin care products. At the same time, the turmeric gel added to the composition has a soothing effect while whitening, and can reduce the irritation of the effective ingredients to the skin.

[0045] The invention selects raw materials, taking into full consideration the stability and usability of the raw materials. The composition obtained by the invention has good stability and solubility, low irritation, and is easy to formulate. It can be added as a raw material to finished cosmetics for preparing cosmetics with whitening and firming effects. DETAILED DESCRIPTION

[0046] In the embodiment of the present invention, each group of experiments is set up with face cream as an example, but it is not limited to the use of face cream. The composition can also be applied to various skin care products such as emulsion, essence, freeze-dried powder, skin care water, essence, etc. The face cream is the above key combination and auxiliary ingredients such as moisturizer, skin conditioner, thickener, antioxidant, excipient, emulsifier and pH regulator. Preferably, the raw materials for preparing the face cream with whitening and firming effects also include any one or a combination of at least two of preservatives and flavors.

[0047] In one embodiment, a facial cream is provided, wherein the facial cream comprises an E phase consisting of a whitening and firming composition, and further comprises an A phase auxiliary material, a B phase auxiliary material, a C phase auxiliary material, and a D phase auxiliary material, based on 100 parts by weight of the facial cream;

[0048] The phase A auxiliary material includes 0.1 to 15 parts of xanthan gum, 0.1 to 15 parts of carbomer, 0.01 to 1.5 parts of sodium hyaluronate, 0.1 to 10 parts of allantoin, 5 to 25 parts of glycerin and 1 to 10 parts of butylene glycol;

[0049] Based on 100 parts by weight of the facial cream, the phase B auxiliary materials include 1-15 parts of cetyl alcohol, 1-15 parts of stearyl alcohol, 1-15 parts of behenyl alcohol, 0.1-15 parts of cetearyl glucoside (and) cetearyl alcohol, 1-10 parts of squalane, 1-10 parts of avocado tree (BUTYROSPERMUM PARKII) fruit fat, 0.1-5 parts of tocopherol acetate, 1-5 parts of hydrogenated polyisobutene, and 1-10 parts of polydimethylsiloxane;

[0050] Based on 100 parts by weight of the facial cream, the C-phase auxiliary material includes 0.1 to 5 parts of arginine;

[0051] Based on 100 parts by weight of the facial cream, the phase D auxiliary material includes 0.1 to 1 part of phenoxyethanol and 0.1 to 5 parts of essence.

[0052] Based on 100 parts by weight of the facial cream, the E phase is 1 to 7.8 parts, preferably 3.2 to 7.8 parts. The whitening and firming composition constituting the E phase is composed of bilberry polyphenol, turmeric gel and olive extract, and the mass ratio of the bilberry polyphenol, turmeric gel and olive extract is (0.1-0.825): (0.1-3): (0.1-5), preferably (0.1-0.5): (0.1-2): (0.1-3).

[0053] One or more embodiments of the present application provide a method for preparing a facial cream, comprising the following steps:

[0054] a. Water phase: After fully moistening and mixing the auxiliary materials of phase A, add them into water, place in a container with a stirring device, stir thoroughly until completely dissolved, and heat to obtain a water phase.

[0055] b. Oil phase: Mix and heat the auxiliary materials of phase B, and stir until completely dissolved to obtain the oil phase.

[0056] c. Emulsification: Add the oil phase obtained in step b to the water phase in step a, and stir and homogenize for 5 minutes.

[0057] d. Add phase C auxiliary materials, stir and homogenize for 3 minutes. Stir and cool to 45℃.

[0058] e. Add phase E consisting of the whitening and firming composition, stir and homogenize for 3 minutes.

[0059] f. After stirring and cooling to 35-38℃, add phase D auxiliary materials, stir and homogenize for 3 minutes, filter and sterilize before filling, test the bacterial content and perform quality control on toxins and immunogenicity to obtain the desired whitening and firming cream.

[0060] Furthermore, the heating temperature in steps a to b is 75 to 80° C.;

[0061] Furthermore, the stirring speed in steps c to f is 200 to 400 rpm; further, the homogenizing speed in steps c to f is 2000 to 4000 rpm. The present invention will be described in detail below in conjunction with specific embodiments.

[0062] Example 1

[0063] This embodiment provides a facial cream, the ingredients of which are shown in Table 1.

[0064] Table 1 Facial cream ingredients of Example 1

[0065]

[0066]

[0067] The preparation method of the facial cream of this embodiment comprises the following steps:

[0068] a. Obtaining the aqueous phase: Place water in a container with a stirring device, fully wet and mix the other parts of the raw material combination A, add them into the water, stir thoroughly until completely dissolved, and heat to 75-80°C.

[0069] b. Oil phase: Mix raw material combination B, heat to 75-80°C, and stir until completely dissolved.

[0070] c. Emulsification: Add the mixture of step b to the aqueous phase of step a, stir and homogenize for 5 minutes, the stirring speed is 300 rpm, and the homogenization speed is 3000 rpm.

[0071] d. Add raw material C, stir and homogenize for 3 minutes at a stirring speed of 300 rpm and a homogenizing speed of 3000 rpm. Stir and cool to 45°C.

[0072] e. Add raw material combination E, stir and homogenize for 3 minutes, the stirring speed is 300 rpm, and the homogenization speed is 3000 rpm.

[0073] f. After stirring and cooling to 35-38°C, add raw material combination D, stir and homogenize for 3 minutes at a stirring speed of 300rpm and a homogenizing speed of 3000rpm. Filter and sterilize before filling, test the bacterial content, and perform quality control on toxins, immunogenicity, etc. to obtain the desired whitening and firming facial cream.

[0074] Examples 2-3 and Comparative Examples 1-7

[0075] The difference between Example 2 and Example 1 is only the increase of each effective ingredient, and the difference between Example 3 and Example 1 is only the reduction of each effective ingredient.

[0076] The difference between Control Examples 1-3 and Example 3 is the lack of one key composition component, the difference between Control Examples 4-6 and Example 3 is the lack of two key composition components, and the difference between Control Example 7 and Example 3 is that no composition component is added.

[0077] The specific proportions of Examples 2-3 and Comparative Examples 1-7 refer to Table 2, and the preparation method is consistent with that of Example 1.

[0078] Table 2 Facial cream ingredients of the embodiments and control examples

[0079]

[0080]

[0081]

[0082] Efficacy evaluation test

[0083] Table 3 Cell experiment raw material composition ratio

[0084]

[0085] 1. Determination of tyrosinase activity in mouse melanoma B16 cells

[0086] Test product: Cell experimental raw material solution obtained from Experimental Examples 1 to 3 and Experimental Control Examples 1 to 7 Model: Mouse melanoma B16 cells

[0087] Test method:

[0088] 1) B16 cell culture and grouping

[0089] Mouse melanoma B16 cells were placed in a medium containing 10% fetal bovine serum and penicillin-streptomycin (100 kU·L -1 ) in DMEM high-glucose medium (pH=7.2) at 37°C, 5% CO2 and relative saturated humidity, and cells in the logarithmic growth phase were used for subsequent experiments;

[0090] 2) Determination of intracellular tyrosinase activity

[0091] B16 cells were taken at 5×10 7 ·L -1 Inoculate in 12-well plates, 1 mL per well, and culture for 24 h. Add 0.2 μmol·L -1 α-MSH was used to induce and construct a melanin high-expression cell model, and then different test samples were added, with 4 replicates in each group. After 48 hours of culture, the supernatant was discarded, washed with PBS three times, 500 μL of PBS buffer containing 1% TritonX-100 was added to each well, placed in a -80℃ refrigerator for 1 hour, and then thawed at room temperature. The cell lysate was centrifuged at 12 000r·min-1 and 4℃ for 20 minutes, and the supernatant was collected. 60 μL of the supernatant was taken in a 96-well plate, 140 μL of 5mmol·L-1L-Dopa was added, incubated at 37℃ for 1 hour, and the absorbance of each well was measured at a wavelength of 405nm. The protein content in the solution was determined by the Bradford method. The relative activity of intracellular tyrosinase was calculated according to the following formula: relative activity of intracellular tyrosinase / % = tyrosinase activity per gram of protein in the experimental group / tyrosinase activity per gram of protein in the α-MSH model group × 100%.

[0092] 2. Free radical (DPPH) scavenging test

[0093] Test product: Cell experimental raw material solution obtained from Experimental Examples 1 to 3 and Experimental Control Examples 1 to 7

[0094] Experimental methods:

[0095] 1) Prepare 0.1 mmol / L DPPH solution and store in dark place; prepare 100 mg / mL solutions of different samples;

[0096] 2) Place on plate and react at room temperature in the dark for 30 minutes.

[0097] Sample group: sample solution 100 μL + DPPH solution 100 μL;

[0098] Blank group: 100 μL sample solution + 100 μL anhydrous ethanol;

[0099] Control group: 100 μL DPPH solution + 100 μL water;

[0100] 3) Detect the absorbance at 517 nm, take the average value, and calculate the DPPH clearance rate of each sample, where the DPPH clearance rate calculation formula is: (1-(Asample-Ablank) / Acontrol)×100%

[0101] Table 4 Comparison of the performance of the cell experimental raw material solutions obtained in Experimental Examples 1 to 3 and Experimental Control Examples 1 to 7

[0102]

[0103] As shown in Table 4, Experimental Examples 1-3 and Experimental Control Examples 1-2 have significant inhibitory effects on tyrosinase and can reduce the production of melanin. Compared with Experimental Control Examples 1-2, Experimental Examples 1-3 have more significant inhibitory effects on tyrosinase and the ability to scavenge free radicals. From the comparison of the results, it can be seen that compared with not using the whitening and firming composition and only using one or two of bilberry polyphenols, turmeric gel and olive extract, the inhibitory effects on tyrosinase and the ability to scavenge free radicals of bilberry polyphenols, turmeric gel and olive extract are more significant when used simultaneously, indicating that the composition of bilberry polyphenols, turmeric gel and olive extract has a synergistic effect, and the facial cream prepared from the composition has good whitening and free radical scavenging abilities.

[0104] 3. Human trials - skin elasticity

[0105] Test products: the whitening and firming facial cream obtained in Example 3 and the facial cream obtained in Control Example 1.

[0106] Test method: On the 0th day, the subjects cleansed their faces with a facial cleanser and wiped their skin with dry facial tissue. After sitting in a laboratory at a temperature of 21±1°C and 50±10% RH for 30 minutes, the observed parts of the subjects were measured by Cutometer for relevant indicators. Thereafter, the product of Example 3 and the product of Control Example 1 were used on the left and right sides of the cheeks after cleansing twice a day, morning and evening. On the 7th day and the 28th day, the observed parts of the subjects were measured by Cutometer for relevant indicators, and statistical analysis software was used to perform statistical analysis of the data.

[0107] 4. Human trials - skin firmness

[0108] Test products: the whitening and firming facial cream obtained in Example 3 and the facial cream obtained in Control Example 1.

[0109] Test method: On the 0th day, the subjects cleansed their faces with a facial cleanser and wiped their skin with dry facial tissue. After sitting in a laboratory at a temperature of 21±1°C and 50±10% RH for 30 minutes, the observed parts of the subjects were measured by Cutometer for relevant indicators. Thereafter, the product of Example 3 and the product of Control Example 1 were used on the left and right sides of the cheeks after cleansing twice a day, morning and evening. On the 7th day and the 28th day, the observed parts of the subjects were measured by Cutometer for relevant indicators, and statistical analysis software was used to perform statistical analysis of the data.

[0110] 5. Human body test - crow's tail wrinkle area detection

[0111] Test products: the whitening and firming facial cream obtained in Example 3 and the facial cream obtained in Control Example 1.

[0112] Test method: On the 0th day, the subjects cleansed their faces with a facial cleanser and wiped their skin with dry facial tissue. After sitting in a laboratory at a temperature of 21±1°C and 50±10% RH for 30 minutes, relevant indexes of the observed parts of the subjects were measured by VISIA. Thereafter, the product of Example 3 and the product of Control Example 1 were used on the left and right sides of the cheeks after cleansing twice a day, morning and evening. Relevant indexes of the observed parts of the subjects were measured by VISIA on the 7th day and the 28th day, and statistical analysis software was used to perform statistical analysis of the data.

[0113] Table 5 Comparison results of the performance of the whitening and firming cream obtained in Example 3 before and after testing

[0114]

[0115] Table 6 Correlation results of performance test of whitening and firming cream obtained in Example 3

[0116]

[0117] Table 7 Results of the correlation test on the performance of the cream obtained in Comparative Example 1

[0118]

[0119] Table 8 Results of the correlation test on the performance of the cream obtained in Comparative Example 1

[0120]

[0121]

[0122] *Statistical method: For normal distribution, paired T-test was used; for non-normal distribution, the Wilcoxon signed-rank test for two related samples was used. The significance level α = 0.05.* Significance annotation method: "n.s." indicates no significant difference, p > 0.05; "*" indicates significant difference, 0.01 < p ≤ 0.05; "**" indicates very significant difference, 0.001 < p ≤ 0.01; "***" indicates extremely significant difference, p ≤ 0.001.

[0123] Based on Tables 4 - 8, the results show that:

[0124] (1) After continuous use of the test product of Example 3 for 7 days and 28 days, the skin elasticity of the subjects showed a significant increase compared with the baseline value. The change rate of the skin elasticity of the subjects after continuous use of the test product for 7 days compared with the baseline value was 4.94, and the change rate of the skin elasticity of the subjects after continuous use of the test product for 28 days compared with the baseline value was 6.82. This indicates that the whitening and firming cream has a good effect on improving skin elasticity, and the effect is better than that of Comparative Example 1;

[0125] (2) After continuous use of the test product of Example 3 for 7 days and 28 days, the skin firmness of the subjects showed a significant increase compared with the baseline value. The change rate of the skin firmness of the subjects after continuous use of the test product for 7 days compared with the baseline value was 7.07, and the change rate of the skin firmness of the subjects after continuous use of the test product for 28 days compared with the baseline value was 9.09. This indicates that the whitening and firming cream has a good effect on improving skin firmness, and the effect is better than that of Comparative Example 1;

[0126] (3) After continuous use of the test product of Example 3 for 7 days, the wrinkle area of the crow's feet of the subjects showed a significant decrease compared with the baseline value. The change rate of the wrinkle area of the crow's feet of the subjects after continuous use of the test product for 7 days compared with the baseline value was -3.43%; after continuous use of the test product for 28 days, the wrinkle area of the crow's feet of the subjects showed a very significant decrease compared with the baseline value. The change rate of the wrinkle area of the crow's feet of the subjects after continuous use of the test product for 28 days compared with the baseline value was -5.33%. This indicates that the whitening and firming cream has a good effect on reducing the wrinkle area of the crow's feet, and the effect is better than that of Comparative Example 1.

[0127] The above description of the embodiments is to facilitate the understanding and use of the invention by those skilled in the art. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the present invention is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the present invention should be within the scope of protection of the present invention.

Claims

1. A whitening and firming composition, characterized in that: The invention comprises bilberry polyphenol, turmeric gel and olive extract, wherein the mass ratio of bilberry polyphenol, turmeric gel and olive extract is (0.1-0.825): (0.1-3): (0.1-5).

2. A whitening and firming composition according to claim 1, characterized in that: The mass ratio of the bilberry polyphenol, turmeric gel and olive extract is (0.1-0.5): (0.1-2): (0.1-3).

3. Use of the whitening and firming composition according to claim 1 or 2 in the preparation of skin care products, characterized in that: The skin care products include lotion, cream, freeze-dried powder, skin care water and essence.

4. A skin care product with whitening and firming effects, characterized in that: It comprises base materials, auxiliary materials and functional ingredients, and the functional ingredients include the whitening and firming composition according to claim 1 or 2.

5. The skin care product with whitening and firming effects according to claim 4, characterized in that: Based on 100 parts by weight of the total weight of the skin care product, the whitening and firming composition is 1 to 7.8 parts.

6. The skin care product with whitening and firming effects according to claim 5, characterized in that: Based on 100 parts by weight of the total weight of the skin care product, the whitening and firming composition is 3.2 to 7.8 parts.

7. The skin care product with whitening and firming effects according to claim 4, characterized in that: The invention also includes auxiliary materials that can be added to the skin care products, wherein the auxiliary materials are selected from one or a combination of thickeners, skin conditioners, moisturizers, antioxidants, excipients, emulsifiers, pH regulators, fragrances or preservatives.

8. The skin care product with whitening and firming effects according to claim 4, characterized in that: The skin care product with whitening and firming effects is a face cream with whitening and firming effects, the face cream comprises an E phase consisting of a whitening and firming composition, and also comprises an A phase auxiliary material, a B phase auxiliary material, a C phase auxiliary material and a D phase auxiliary material; Based on 100 parts by weight of the facial cream, the phase A auxiliary material includes 0.2 to 25 parts of thickener A, 0.11 to 11 parts of skin conditioner, and 6 to 30 parts of moisturizer; Based on 100 parts by weight of the facial cream, the phase B auxiliary material includes 3 to 25 parts of thickener B, 0.1 to 15 parts of emulsifier, 0.1 to 5 parts of antioxidant and 4 to 20 parts of excipient; Based on 100 parts by weight of the facial cream, the phase C auxiliary material includes 0.1 to 5 parts of a pH regulator; Based on 100 parts by weight of the facial cream, the phase D auxiliary material includes 0.1 to 5 parts of a fragrance and 0.15 to 1 part of a preservative; Based on 100 parts by weight of the facial cream, the E phase accounts for 1 to 7.8 parts.

9. The skin care product with whitening and firming effects according to claim 8, characterized in that: The phase A auxiliary material also includes water, and the volume of the water is such that the weight parts of the facial cream reaches 100 parts.

10. The skin care product with whitening and firming effects according to claim 8, characterized in that: The thickener A and thickener B are independently selected from one or more of acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, polyacrylamide, xanthan gum, propylene glycol, behenyl alcohol, carbomer, laureth-7, cetyl alcohol, stearyl alcohol or hydroxyethyl cellulose; The skin conditioning agent is selected from one or more of isohexadecane, olive oil, sodium hyaluronate, lauryl alcohol, avocado butter, allantoin, squalane, panthenol, lactic acid, tocopherol or dipotassium glycyrrhizinate; The moisturizing agent is selected from one or more of hyaluronic acid, allantoin, glycerin, ceramide, sodium lactate, butylene glycol, hydrogenated grape seed oil, olive oil or shea butter; The antioxidant is selected from one or more of tocopherol, tocopherol acetate, grape seed oil, and ascorbic acid; The excipient is selected from one or more of squalane, cholesterol, hydrogenated poly (C6-14 olefin), hydrogenated wheat germ oil hydrogenated polyisobutene, avocado tree (BUTYROSPERMUM PARKII) fruit fat, caprylic / capric triglyceride, and polydimethylsiloxane; The emulsifier is selected from one or more of glyceryl stearate citrate, cetearyl glucoside (and) cetearyl alcohol, behenyl trimethyl ammonium methyl sulfate, cocoglyceryl esters or sorbitan olivate; The pH regulator is selected from one or more of arginine or lactic acid; The aromatic agent includes essence; The preservative is selected from one or more of phenoxyethanol, ethylhexylglycerin and pentylene glycol.

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