Plant sunscreen cream with anti-ultraviolet efficacy and application thereof in sunscreen products

Abstract

The application discloses a plant sunscreen cream with anti-ultraviolet efficacy and application thereof in sunscreen products, and belongs to the field of cosmetic formulations. The plant sunscreen cream with anti-ultraviolet efficacy comprises a plant composition with anti-ultraviolet efficacy, an auxiliary material and a solvent. The plant composition with anti-ultraviolet efficacy comprises polygonatum cyrtonema rhizome / root extract, honeysuckle flower extract, sophora flower bud extract, centella asiatica extract and buddleja officinalis extract. The plant composition provided by the application has a good antioxidant effect and can effectively resist ultraviolet damage and play a photoprotective role, and the sunscreen cream containing the plant composition with anti-ultraviolet efficacy has excellent sunscreen efficacy.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic products, specifically relating to a plant-based sunscreen lotion with anti-ultraviolet properties and its application in sunscreen products. Background Technology

[0002] With global climate change and increased outdoor activities, the damage caused by ultraviolet (UV) radiation to the skin is receiving growing attention. Traditional sunscreens primarily rely on chemical or physical sunscreens to block or absorb UV rays, but these ingredients can cause skin irritation, allergies, or other adverse reactions. Therefore, developing safe, effective, and skin-friendly new sunscreens has become a current research hotspot.

[0003] In recent years, plant extracts have been widely used in the cosmetics industry due to their natural origin, low irritation, and multi-functional properties. Many plants contain active ingredients with anti-UV effects, such as polyphenols, flavonoids, and carotenoids. These ingredients can absorb, scatter, or convert ultraviolet rays, thereby reducing the damage of ultraviolet rays to the skin. In addition, plant extracts also have various skin care benefits such as anti-oxidation, anti-inflammation, and moisturizing, which can improve skin condition and enhance the skin's defense capabilities.

[0004] However, the anti-UV effects of single plant extracts are often limited, and the interactions between different plant extracts may also affect sun protection efficacy. Therefore, how to screen and optimize plant compositions to achieve synergistic effects and improve anti-UV efficacy has become a current research challenge and focus.

[0005] Currently, there are some sunscreen products on the market containing plant extracts, but these products often suffer from problems such as unstable sun protection effects, complex formulations, and high costs. Therefore, developing a plant-based composition with stable anti-UV efficacy, a simple formulation, and reasonable cost, and its application in sunscreen products, is of great significance. Summary of the Invention

[0006] In view of the shortcomings of the prior art, the purpose of this invention is to provide a plant composition with anti-ultraviolet effect, a sunscreen lotion and its application in sunscreen products. The plant composition achieves synergistic effect and improves the anti-ultraviolet effect through the combination and scientific ratio of various plant extracts with anti-ultraviolet effect.

[0007] To achieve the above objectives, the present invention discloses the following technical solutions:

[0008] In a first aspect, the present invention provides a plant composition with anti-ultraviolet effects, comprising rhizome / root extract of Polygonatum sibiricum, extract of Lonicera japonica, extract of Sophora japonica buds, extract of Centella asiatica, and extract of Buddleja officinalis.

[0009] Preferably, the product comprises the following components in parts by mass:

[0010]

[0011] Secondly, the present invention provides a plant-based sunscreen lotion with anti-ultraviolet (UV) properties, comprising the plant composition with UV-resistant properties described in the first aspect.

[0012] Preferably, the amount of the plant composition with anti-ultraviolet effect added to the plant sunscreen lotion is 0.015-0.02 wt%.

[0013] More preferably, it also includes excipients and solvents.

[0014] The excipients include film-forming agents, emulsifying stabilizers, thickeners, humectants, preservatives, antioxidants, and sunscreens, and the solvent is deionized water.

[0015] More preferably, the film-forming agent is at least one selected from triethoxyoctylsilane, octyl polymethylsiloxane, polydimethylsiloxane, PEG-10 polydimethylsiloxane, and trimethylsiloxysilicate;

[0016] The emulsifying stabilizer is at least one of the following: potassium cetyl phosphate, arachidonic acid, behenyl alcohol, arachidonic acid glucoside, cetearyl alcohol, cocoyl glucoside, diisopropyl sebacate, and polyhydroxystearic acid;

[0017] The thickener is at least one of the following: ammonium acryloyldimethyltaurate / VP copolymer, vinyl polydimethylsiloxane / polymethylsiloxane silsesquioxane cross-linked polymer, silica, and distearate dimethylammonium lithium montmorillonite;

[0018] The moisturizer is at least one selected from sunflower seed oil, glyceryl caprylate, 1,2-hexanediol, panthenol, butylene glycol, and 1,2-pentanediol;

[0019] The sunscreen agent is at least one of p-methoxycinnamate, bis-ethylhexyloxyphenol methoxyphenyl triazine, methylene bis-benzotriazolyl tetramethyl butylphenol, butyl octyl salicylate and titanium dioxide.

[0020] The antioxidant is vitamin E;

[0021] The preservative is at least one of p-hydroxyacetophenone and 1,2-hexanediol.

[0022] Thirdly, the present invention provides a method for preparing the plant-based sunscreen lotion described in the second aspect, comprising the following steps:

[0023] The plant-based sunscreen emulsion is obtained by mixing the film-forming agent, emulsifying stabilizer, thickener, moisturizer, preservative, antioxidant, sunscreen agent, the plant composition, and deionized water at 40-80°C until homogeneous.

[0024] Fourthly, the present invention provides the use of the plant composition with anti-ultraviolet effects described in the first aspect in the preparation of sunscreen lotion, sunscreen cream, sunscreen liquid, sunscreen spray, sunscreen gel, isolation cream, isolation lotion or BB cream.

[0025] In this invention:

[0026] The rhizome / root extract of Polygonatum multiflorum is rich in active ingredients such as polysaccharides, steroidal saponins, triterpenes, alkaloids, lignans, flavonoids, phytosterols and volatile oils. It has a certain absorption capacity for ultraviolet rays and can scavenge reactive oxygen free radicals induced by ultraviolet radiation, thus providing a certain degree of protection against ultraviolet rays.

[0027] Honeysuckle flower extract contains active ingredients such as luteolin, luteolinoside, chlorogenic acid and its isomers, and triterpenoid saponins. The flavonoids in honeysuckle flower extract can absorb ultraviolet energy, thereby reducing the direct damage of ultraviolet rays to the skin. At the same time, the chlorogenic acid and flavonoids in honeysuckle flower extract have strong antioxidant capacity, which can effectively remove free radicals generated by the skin after being exposed to ultraviolet rays, reduce the damage caused by oxidative stress to the skin, and indirectly protect the skin from ultraviolet damage.

[0028] Sophora japonica flower bud extract contains abundant flavonoids, isoflavones, saponins and other active ingredients, which have a certain ability to resist ultraviolet rays. At the same time, Sophora japonica flower bud extract can reduce oxidative stress damage and inflammatory response caused by ultraviolet rays, thus indirectly playing a role in resisting ultraviolet damage.

[0029] Centella asiatica extract is rich in active ingredients such as triterpenoid saponins, polysaccharides, and flavonoids, which can effectively promote wound healing and repair damaged skin. It has significant anti-inflammatory effects, effectively reducing skin redness and pain. At the same time, it can effectively scavenge free radicals, protect the skin from environmental damage, and delay aging.

[0030] The flavonoids in Buddleja officinalis extract have a strong broad-spectrum absorption capacity for ultraviolet light, and can directly absorb ultraviolet radiation, thereby exerting an anti-ultraviolet radiation effect. At the same time, Buddleja officinalis extract can scavenge reactive oxygen free radicals induced by ultraviolet radiation, and reduce oxidative damage and inflammatory response caused by ultraviolet light.

[0031] The beneficial effects of this invention are:

[0032] 1. In the composition provided by the present invention, the rhizome / root extract of Polygonatum odoratum, extract of Lonicera japonica, extract of Sophora japonica bud, extract of Centella asiatica and extract of Buddleja officinalis complement each other and have a synergistic effect, resulting in good antioxidant effect;

[0033] 2. The rhizome / root extract of Polygonatum odoratum, Lonicera japonica flower extract, Sophora japonica flower bud extract, Centella asiatica extract and Buddleja officinalis flower extract of the present invention can effectively resist ultraviolet damage and play a photoprotective role;

[0034] 3. The sunscreen lotion containing a composition of Polygonatum odoratum rhizome / root extract, Lonicera japonica flower extract, Sophora japonica flower bud extract, Centella asiatica extract and Buddleja officinalis flower extract provided by the present invention has excellent sun protection efficacy. Detailed Implementation

[0035] To better illustrate the objectives, technical solutions, and advantages of this invention, the invention will be further described below with reference to specific embodiments. Those skilled in the art should understand that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

[0036] Unless otherwise specified, the experimental methods used in the specific embodiments are all conventional methods; the materials and reagents used are all commercially available unless otherwise specified; and the percentages mentioned in the specific embodiments are all mass percentages unless otherwise specified.

[0037] In this invention:

[0038] The rhizome / root extract of Polygonatum multiflorum was purchased from Jiangsu Duoyang Bioengineering Technology Co., Ltd., with a specification of 10:1, and is a water-soluble powder.

[0039] The honeysuckle flower extract is a water-soluble powder made from honeysuckle (LONICERA JAPONICA) flowers, purchased from Shaanxi Xintianyu Biotechnology Co., Ltd., with a specification of 10:1.

[0040] The Sophora japonica flower bud extract was purchased from Huzhou Purui Biomedical Technology Co., Ltd., with a ratio of 10:1 and a processing method of water extraction.

[0041] Centella Asiatica extract is an extract of Centella Asiatica, purchased from Sinopharm (Guangzhou) International Pharmaceutical and Health Co., Ltd., with a purity of 30% and a processing method of water extraction.

[0042] The extract of Buddleja officinalis is an extract of Buddleja officinalis, purchased from Xi'an Senran Bioengineering Co., Ltd., with a specification of 10:1, and is a water-soluble powder.

[0043] Other raw materials are available commercially.

[0044] Preparation of plant compositions:

[0045] The raw materials were accurately weighed according to the mass fractions in Table 1, and then placed in a high-speed mixer for uniform mixing to obtain the plant composition.

[0046] Table 1. Mass parts of raw materials for plant composition

[0047]

[0048]

[0049] Note: "-" in the table indicates no additions.

[0050] Performance testing of plant compositions:

[0051] Take the above compositions 1-8 and mix them with deionized water to prepare a test sample with a concentration of 0.02wt%.

[0052] 1. Antioxidant test:

[0053] This test assesses the DPPH free radical scavenging ability of the test samples of compositions 1-8. The ability to scavenge DPPH free radicals reflects, to some extent, the antioxidant capacity of a substance.

[0054] The testing method is as follows:

[0055] Accurately weigh the DPPH reagent and prepare a 0.04 mg / mL DPPH solution using 50 v / v ethanol aqueous solution. Under light-protected conditions, add the DPPH solution and different test samples to a 96-well plate, mix thoroughly, and incubate at room temperature in the dark for 30 minutes. Measure the absorbance at 517 nm using a microplate reader. Each measurement requires three groups: sample, blank, and control, with three replicates per group, as detailed below:

[0056] Sample set: 100 μL sample solution + 100 μL DPPH solution

[0057] Blank group: 100 μL sample solution + 100 μL 50 v / v ethanol aqueous solution

[0058] Control group: 100 μL deionized water + 100 μL DPPH solution

[0059] Calculate the DPPH removal rate using the following formula:

[0060] DPPH clearance rate = [1 - (sample group - blank group) / control group] × 100%

[0061] The test results are shown in Table 2:

[0062] Table 2 Antioxidant Test Results

[0063] Group DPPH removal rate / % Composition 1 89.36 Composition 2 90.11 Composition 3 88.29 Composition 4 44.78 Composition 5 48.42 Composition 6 42.36 Composition 7 31.08 Composition 8 29.28

[0064] Results analysis:

[0065] As shown in Table 2, compositions 1-3 exhibit excellent DPPH free radical scavenging ability. Compared with compositions 4-8, the absence of any one or two components reduces the free radical scavenging rate of the compositions. This indicates that the extracts of Polygonatum odoratum rhizome / root, Lonicera japonica flower, Sophora japonica flower bud, Centella asiatica, and Buddleja officinalis in the compositions complement each other and have a synergistic effect, resulting in good antioxidant effects.

[0066] 2. Light protection test:

[0067] This test examined the cell viability of compositions 1-8. The survival rate of immortalized human keratinocytes (HaCat) after UV irradiation can reflect the protective effect of substances against UV radiation to a certain extent.

[0068] The testing method is as follows:

[0069] First, the cell safety test concentration of the composition is determined by the MTT assay for cytotoxicity.

[0070] According to 2×10 5 Immortalized keratinocyte suspension was seeded into 96-well plates at a density of 100 μL / well and incubated at 37°C in a 5% CO2 incubator for 24 h.

[0071] Using complete culture medium (DMEM high glucose complete culture medium, catalog number MA10001A, purchased from Beijing Anruit Biotechnology Co., Ltd.) as the diluent, the test samples of composition 1-8 were diluted to the cell safety test concentration and then added to the cells, 100 μL / well. The positive control group and the negative control group were replaced with the same mass of deionized water. The cells were incubated at 37°C in a CO2 incubator with a volume fraction of 5% for 24 h.

[0072] Cells from groups 1-8 of the composition and the positive control group were irradiated with UV light for 20 min, while the negative control group was not treated with light. The cells were then incubated in an incubator for 24 h, and the changes in cell viability were detected by the MTT assay.

[0073] The calculation formula is as follows:

[0074] Cell viability percentage (%) = (Average OD value of sample wells / Average OD value of negative control group) × 100%

[0075] The test results are shown in Table 3:

[0076] Table 3. Results of light protection test

[0077]

[0078] Results analysis:

[0079] As shown in Table 3, compared with the positive control group, the HaCat cells treated with compositions 1-3 showed significantly increased activity after ultraviolet irradiation, and were higher than those of compositions 4-8. This indicates that the compositions of the present invention can effectively resist ultraviolet damage and play a photoprotective role.

[0080] Preparation of sunscreen lotion:

[0081] Step S1-1. Weigh the raw materials accurately according to the mass percentage of the raw materials in Table 4. Place each raw material in component A into an emulsification reaction vessel and dissolve and mix it evenly at 80℃ and 200r / min to obtain mixture A.

[0082] Step S1-2. Take the deionized water of component B in Table 4 and place it in a reaction vessel and heat it to 80°C. Add glyceryl caprylate, 1,2-hexanediol, panthenol, butanediol, 1,2-pentanediol, and vinyl polydimethylsiloxane / polymethylsiloxane sesquisiloxane crosslinked polymer in sequence. Stir and mix evenly at 200 r / min. After mixing evenly, increase the speed to 500 r / min, add silica and distearate dimethylammonium lithium montmorillonite, and mix evenly to obtain mixture B.

[0083] Step S1-3. At 80℃, mixture A is pumped into mixture B and rapidly homogenized at 5000 r / min. After homogenization for 10 min, the mixture is mechanically stirred at 200 r / min for 30 min to obtain mixture AB.

[0084] Step S1-4. Dissolve and mix the raw materials of component D to obtain mixture D. After cooling mixture AB to 55℃, add the raw materials of component C and mixture D in sequence, and mix and stir at 500r / min to obtain mixture ABCD.

[0085] Step S1-5. After cooling the mixture ABCD to 40°C, add the plant composition 2 and the remaining deionized water to it, and mix evenly at 200 r / min to obtain sunscreen lotion.

[0086] Table 4 Application Examples: Raw Material Mass Percentage

[0087]

[0088]

[0089] SPF value test for topical agents

[0090] The testing method is as follows:

[0091] I. Materials and Methods

[0092] 1. Test object: Application examples 1-3.

[0093] 2. Reference standard: SPF value ≈ 15.4, prepared according to the standard formula of high SPF standard (P2) in the "Cosmetic Safety Technical Specifications" (2015 edition).

[0094] 3. Subjects: A total of 3 females, aged 34 to 36 years, who met the subject voluntary inclusion criteria.

[0095] 4. Light source: Xenon arc lamp of daylight simulator, all performance indicators meet the requirements of the testing specifications.

[0096] 5. Test methods:

[0097] The test was conducted according to the specific requirements of the currently effective technical specifications. The subject was placed in a prone position, and their back was irradiated. The minimum erythema dose (MED) of the subject's skin to ultraviolet radiation was predicted 24 hours prior to the test, and the ultraviolet radiation dose was adjusted based on the prediction results for testing the analyte.

[0098] On the day of the test, first select a spot on the subject's back that is at least 30cm long. 2 In normal skin areas, at 2.00±0.05 mg / cm² 2 Apply the test substance or control evenly to the above-mentioned area, and then select the irradiation dose according to the standard requirements. Irradiate under three conditions: ① no test substance applied to the subject's skin; ② control substance applied; ③ test substance applied. Observe the experimental results after 24 hours and record the MED values ​​under each of the three conditions.

[0099] 6. SPF value calculation method:

[0100] The SPF value of the analyte or control for protecting a single subject is expressed by the following formula:

[0101] SPF = MED value of protected skin / MED value of unprotected skin.

[0102] Individual SPF values ​​must be accurate to one decimal place. The arithmetic mean of the SPF values ​​of all subjects protected by the test substance is calculated, and the integer part is taken as the SPF value of the sample being tested.

[0103] 7. The test results are shown in Table 5:

[0104] Table 5 SPF Value Test Results

[0105]

[0106] PFA value test for topical agents

[0107] The testing method is as follows:

[0108] 1. Test object: Application examples 1-3.

[0109] 2. Reference standard: PFA value ≈ 4.1, prepared according to the standard formula of the reference standard in the "Cosmetic Safety Technical Specifications" (2015 edition).

[0110] 3. Subjects: A total of 3 females, aged 30 to 36 years, who met the subject voluntary inclusion criteria.

[0111] 4. Light source: Xenon arc lamp of daylight simulator, all performance indicators meet the requirements of the testing specifications.

[0112] 5. Test Method: The test shall be conducted in accordance with the specific requirements of the currently effective technical specifications. The subject shall lie prone, and their back shall be irradiated. The minimum melanization potential (MPPD) of the subject's skin to ultraviolet radiation shall be predicted 24 hours prior to the test, and the ultraviolet radiation dose shall be adjusted based on the prediction result for testing the analyte. The test shall first be conducted on a point at least 30cm long on the subject's back. 2 For normal skin areas, administer (2.00±0.05) mg / cm². 2 Apply the test substance or control evenly to the above-mentioned area; then select the UVA irradiation dose according to the standard requirements and irradiate under three conditions: ① no test substance applied to the subject's skin; ② application of control; ③ application of test substance. Observe the experimental results after 2-4 hours and record the MPPD values ​​under each of the three conditions.

[0113] 6. Calculation method of PFA value:

[0114] The PFA value of the analyte or control for protecting a single subject is expressed by the following formula:

[0115] PFA = MPPD value of protected skin / MPPD value of unprotected skin.

[0116] Individual PFA values ​​must be accurate to one decimal place. The arithmetic mean of the PFA values ​​of all subjects protected by the test substance is calculated, and the integer part is taken as the PFA value of the sample being tested.

[0117] 7. The test results are shown in Table 6:

[0118] Table 6. Test Results of PFA Values

[0119]

[0120] Results analysis:

[0121] SPF stands for Sun Protection Factor. The higher the SPF value, the better the protection against sunburn and erythema.

[0122] PFA stands for UVA Protection Index. The higher the PFA value, the better the UVA protection.

[0123] According to the results in Tables 5 and 6, the sunscreen provided by this invention has excellent sun protection efficacy. The combination of Polygonatum odoratum rhizome / root extract, Lonicera japonica flower extract, Sophora japonica flower bud extract, Centella asiatica extract and Buddleja officinalis extract can play a good role in photoprotection against skin application when used in sunscreen products. At the same time, the higher the concentration of the plant composition, the better the sun protection performance of the sunscreen, showing a certain concentration dependence.

[0124] While specific embodiments of the present invention have been described above, those skilled in the art should understand that these are merely illustrative examples, and the scope of protection of the present invention is defined by the appended claims. Those skilled in the art can make various changes or modifications to these embodiments without departing from the principles and essence of the present invention, but all such changes and modifications fall within the scope of protection of the present invention.

Claims

1. A plant composition with anti-ultraviolet (UV) activity, characterized in that, The plant composition comprises, by weight parts, the following components: 25-35 parts of rhizome / root extract of Polygonatum multiflorum; Honeysuckle flower extract 15-25 parts; Sophora japonica bud extract 4.5-5 parts; Centella asiatica extract 15-30 parts; 35-45 parts of Buddleja officinalis extract; The extracts of Polygonatum odoratum rhizome / root, Lonicera japonica flower extract, and Buddleja officinalis flower extract are water-soluble powders. The Sophora japonica bud extract and Centella asiatica extract were prepared by water extraction.

2. A plant-based sunscreen lotion with anti-ultraviolet (UV) properties, characterized in that, The plant-based sunscreen lotion is composed of the plant composition, excipients, and solvents described in claim 1; The excipients include film-forming agents, emulsifying stabilizers, thickeners, humectants, preservatives, antioxidants, and sunscreens; The solvent is deionized water.

3. The plant-based sunscreen lotion according to claim 2, characterized in that, The amount of the plant composition added to the plant-based sunscreen lotion is 0.015-0.02 wt%.

4. The plant-based sunscreen lotion according to claim 2, characterized in that, The film-forming agent is at least one of triethoxyoctylsilane, octyl polymethylsiloxane, polydimethylsiloxane, PEG-10 polydimethylsiloxane, and trimethylsiloxysilicate; The emulsifying stabilizer is at least one of the following: potassium cetyl phosphate, arachidonic acid, behenyl alcohol, arachidonic acid glucoside, cetearyl alcohol, cocoyl glucoside, diisopropyl sebacate, and polyhydroxystearic acid; The thickener is at least one of the following: ammonium acryloyl dimethyl taurate / VP copolymer, vinyl polydimethylsiloxane / polymethylsiloxane silsesquioxane crosslinked polymer, silica, and distearate dimethylammonium lithium montmorillonite; The moisturizer is at least one selected from sunflower seed oil, glyceryl caprylate, 1,2-hexanediol, panthenol, butylene glycol, and 1,2-pentanediol; The sunscreen agent is at least one of the following: isoamyl p-methoxycinnamate, bis-ethylhexyloxyphenol methoxyphenyl triazine, methylene bis-benzotriazolyl tetramethylbutylphenol, butyl octyl salicylate, and titanium dioxide.

5. The method for preparing the plant-based sunscreen lotion according to claim 4, characterized in that, Includes the following steps: The plant-based sunscreen emulsion is obtained by uniformly mixing the film-forming agent, emulsifying stabilizer, thickener, moisturizer, preservative, antioxidant, sunscreen agent, the plant composition, and deionized water at 40-80°C.

6. The use of the plant composition with anti-ultraviolet effect as described in claim 1 in the preparation of sunscreen lotion, sunscreen cream, sunscreen liquid, sunscreen spray, sunscreen gel, isolation cream, isolation lotion or BB cream.

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