Compound microbial agent for promoting yield and income increase of wheat and preparation method of compound microbial agent

Abstract

The invention discloses a compound microbial agent for promoting yield and income increase of wheat and a preparation method of the compound microbial agent, and belongs to the technical field of microorganisms. The fertilizer is prepared from 100-150 parts of a microbial agent, 10-20 parts of a nutrient solution, 20-30 parts of a plant extract and 3-5 parts of an accelerant. According to the invention, the three strains have a synergistic effect to jointly achieve the effects of promoting the growth of wheat and improving the yield and quality of wheat, and meanwhile, the occurrence of diseases can be effectively reduced; the plant extracting solution used by the invention contains the momordica grosvenori seed extract and the N-coconut oil acyl-N-sodium methyl taurate, and the momordica grosvenori seed extract and the N-coconut oil acyl-N-sodium methyl taurate act together, so that the high activity and the long-term stability of the compound microbial agent are maintained; the components work together to improve the soil environment, promote nutrient absorption and utilization and improve the growth speed and stress resistance of wheat, so that the yield is increased, the quality is improved, and the fertilizer has a good market application prospect.

Description

Technical Field

[0001] The invention belongs to the technical field of microorganisms, and in particular relates to a composite microbial agent for promoting wheat production and income increase and a preparation method thereof. Background Art

[0002] Wheat is an important commodity grain and strategic reserve grain in my country. According to the National Bureau of Statistics, the total planting area of ​​wheat in various regions of my country has reached 23.52 million hectares, with a total output of 137.7 million tons. According to the FAO survey, the total wheat output in the world in 2022 is about 784 million tons, and my country's wheat output accounts for about 18% of the world's total output, ranking first in the world, playing an important role in global food security. In order to meet the food demand of the world's growing population, researchers continue to use various means to increase wheat production. At present, agricultural production mainly relies on the application of chemical fertilizers to increase wheat production, but the long-term application of chemical fertilizers has caused soil compaction, low fertilizer utilization rate, and excessive carbon emissions. At the same time, a variety of diseases and pests will occur during the planting process of wheat, and effective pest and disease control methods can also increase wheat production. At present, the control of wheat pests and diseases is overly dependent on various chemical pesticides, which has many potential threats and limitations, such as drug resistance, environmental impact, poor durability and low durability. With the warming of the environment, improper use of pesticides, and increased residual pollutants, traditional chemical control methods can no longer meet people's requirements for safe and effective disease prevention. As a result, wheat yield and quality are affected by a variety of pests and diseases and abiotic factors, threatening wheat production and food security.

[0003] Plants interact with various microorganisms during their growth, mainly including plant rhizosphere microorganisms, phyllosphere microorganisms and plant endophytes. These microorganisms have a great impact on soil ecological environment such as soil microbial flora activity, enzyme activity, nutrients, etc. Studies have shown that crops treated with suitable microbial agents significantly increase soil nutrient nitrogen and phosphorus content, urease, phosphatase activity and yield compared to crops not treated with them. Inoculation with composite microbial agents increases soil urease, sucrase, and protease activity, promotes the absorption of mineral nutrients such as nitrogen, phosphorus and potassium, and increases yield.

[0004] At present, the effect of single microbial agents is not ideal, and most of the composite microbial agents are solid agents. Due to the poor synergy of the selected strains or the poor stability of the carrier, the stability and durability of the agents are poor; and liquid composite microbial agents are usually live bacteria products, which have poor stability and effect during product transportation. At the same time, they have poor adaptability to wheat, especially for winter wheat with a long growth cycle. It does not play a corresponding disease resistance and growth promotion role. Therefore, the preparation of a composite microbial agent that has a significant effect on promoting wheat production and income increase and has stable product quality has become a technical problem that needs to be solved urgently. Summary of the invention

[0005] The purpose of the present invention is to provide a composite microbial agent for promoting wheat production and income. The liquid microbial agent is made by selecting suitable strains for cultivation and fermentation, and is supplemented with other nutrient solutions, plant extracts and other ingredients to obtain a composite microbial agent with high microbial activity, stable quality and good growth-promoting and yield-increasing effects on wheat. The market application prospects are good.

[0006] In order to achieve the above technical objectives, the technical solution adopted by the present invention is: A composite microbial agent for promoting wheat yield increase and income increase is prepared from the following raw materials in parts by weight: 100-150 parts of microbial agent, 10-20 parts of nutrient solution, 20-30 parts of plant extract, and 3-5 parts of promoter; the microbial agent is prepared from Streptomyces ruticosa, Bacillus niger, and Bacillus velez; the plant extract is composed of monk fruit seed extract and sodium N-cocoyl-N-methyltaurate in a mass ratio of 5:1; the promoter is composed of polyvinyl alcohol and phytic acid in a mass ratio of 2:1.

[0007] Preferably, the preparation method of the microbial agent is as follows: thaw and activate Streptomyces ruticosa, Bacillus nielsenii, and Bacillus velezii respectively, streak the plates and culture them in an incubator at 28°C for 48 hours, take a loop of the cultured strains in NB liquid culture medium, place it in a shaker at 30°C and 150 r / min for 24 hours to obtain seed liquid; inoculate the seed liquids of the three strains into 200 mL of TSB liquid culture medium at an inoculation rate of 10%, shake and culture at 30°C and 200 r / min for 72 hours to obtain fermentation liquid; mix the three fermentation liquids in a volume ratio of 1:1:1 to obtain the microbial agent.

[0008] The composition of the NB liquid culture medium used was: 5 g of peptone, 2 g of yeast extract, 3 g of beef extract, 5 g of sodium chloride, and 1000 ml of distilled water; pH 7.0, sterilization conditions: 121°C, 20 min.

[0009] The composition of the TSB liquid culture medium used was: 2.5 g yeast extract, 17 g tryptone, 3 g soy peptone, 5 g sodium chloride, 2.5 g glucose, 2.5 g potassium dihydrogen phosphate, 1000 ml distilled water; pH 7.2, sterilization conditions: 121°C, 20 min.

[0010] Preferably, the Streptomyces vinifera, strain number CGMCC No.4.7294, was purchased from the General Microbiology Center of China National Microbiological Culture Collection Administration Committee, and the deposit date was June 26, 2015; the Bacillus nissori, strain number CGMCC No.1.16092, was purchased from the General Microbiology Center of China National Microbiological Culture Collection Administration Committee, and the deposit date was February 13, 2017; the Bacillus velez, strain number CGMCC No.1.12669, was purchased from the General Microbiology Center of China National Microbiological Culture Collection Administration Committee, and the deposit date was September 10, 2013. The Streptomyces vinifera, Bacillus nissori, and Bacillus velezori used in the present invention can all be purchased through the strain catalog of the collection center without the need for additional preservation.

[0011] Preferably, the preparation method of the monk fruit seed extract is as follows: (1) Wash and dry the monk fruit seeds, grind them into powder, and pass them through a 100-mesh sieve to obtain monk fruit seed powder; (2) adding deionized water to the monk fruit seed powder according to the material-liquid ratio, and then adding a composite enzyme solution containing neutral protease and cellulase thereto, adjusting the solution pH to 7.0, performing enzymolysis at 35°C for 6-8h, then inactivating the enzyme at 90°C for 10min, and cooling to room temperature to obtain an enzymolysis solution; (3) Add an equal volume of 25% ethanol to the enzymatic hydrolyzate, adjust the pH of the mixed solution to 5.0-5.5 with acetic acid, heat to 39°C, extract for 90-120 min, centrifuge to obtain the supernatant, and concentrate the supernatant to one third of the original volume to obtain the monk fruit seed extract.

[0012] Preferably, in step (2), the material-liquid ratio of monk fruit seed powder to deionized water is 1 g:30 ml.

[0013] Preferably, the mass volume ratio of the monk fruit seed powder to the complex enzyme solution is 1g:20ml; the neutral protease content in the complex enzyme solution is 20-30g / L, and the cellulase content is 5-10g / L.

[0014] Preferably, the enzyme activity of the neutral protease is 100,000 u / g, and the enzyme activity of the cellulase is 200,000 u / g.

[0015] Preferably, the nutrient solution is composed of urea, trehalose, amino acid chelated zinc, amino acid chelated magnesium, amino acid chelated iron, and deionized water in a mass ratio of 5-7:1-2:0.05:0.02:0.1:100.

[0016] The present invention also provides a method for preparing the composite microbial agent for increasing wheat production and income, which comprises the following steps: (1) Preparation of microbial agents; (2) preparing a monk fruit seed extract, and then mixing it with sodium N-cocoyl-N-methyltaurate according to a mass ratio to prepare a plant extract; (3) Mix the microbial agent with the plant extract, and then homogenize it in a homogenizer for 5-10 minutes, then add the nutrient solution and promoter, and stir evenly to obtain the final product.

[0017] The specific method of using the composite microbial agent for promoting wheat production and income increase prepared by the present invention is: spraying it at the seedling stage. The specific usage is to take the composite microbial agent and dilute it with 50 times the volume of clean water, stir it evenly and then spray it. The amount of the composite microbial agent stock solution per mu is 1L.

[0018] The present invention uses Streptomyces rufipogon, Bacillus nissori and Bacillus velez. Streptomyces rufipogon has the ability to produce antibiotics, fix nitrogen and decompose organic matter, Bacillus nissori has the characteristics of promoting plant growth, disease resistance and stress resistance. Bacillus velez can secrete and produce a variety of biologically active substances, including enzymes, antimicrobial proteins, lipopeptide antibiotics, polyketide antibiotics, plant hormones and other substances. The three strains interact with each other and complement each other, significantly promote the growth of wheat, improve wheat yield and quality, and can also effectively reduce the occurrence of common wheat diseases and insect pests.

[0019] The Momordica grosvenori seed extract prepared by the present invention contains a variety of active ingredients such as polysaccharides, polyphenols, flavonoids, small molecule peptides and amino acids, wherein the polysaccharides can provide abundant energy for microorganisms, which helps to maintain the activity of microorganisms. On the other hand, the active ingredients therein also have an antioxidant effect, can remove free radicals, protect microbial cells from oxidative damage, and help to prolong the survival time of microbial agents, thereby maintaining their stability; at the same time, the active ingredients in the Momordica grosvenori seed extract can also have an inhibitory effect on a variety of pathogens and viruses, and can inhibit the growth and reproduction of harmful microorganisms, thereby reducing the occurrence of diseases and insect pests in wheat planting. And sodium N-coconut oil acyl-N-methyl taurine can reduce the surface tension of the liquid, improve the dispersion effect of the active ingredients in the Momordica grosvenori seed extract in the mixed solution and will not affect the activity, and the combined use of the two further improves the effect of the active ingredients in the Momordica grosvenori seed extract. In addition, the various active ingredients in the Momordica grosvenori seed extract also provide additional energy and nutrient support for wheat, and will affect the metabolic process of wheat, thereby improving the quality of wheat.

[0020] The nutrient solution used in the present invention contains nitrogen, trehalose and various trace elements, which not only provides sufficient nutrients for the reproduction and growth of microorganisms, but also provides rich nutrients for the growth of wheat. The polyvinyl alcohol in the promoter of the present invention contains a large number of hydroxyl groups, which can interact with hydroxyl groups, acid groups and other groups on the surface of microorganisms, thereby achieving the adsorption of microorganisms and fixing the microorganisms in a specific area, improving the effect of the microorganisms, and phytic acid can increase the activity of microorganisms in the area. The two act synergistically to improve the biological effect of the composite microbial agent, promote the absorption and utilization of nutrients by crops, and provide more sufficient nutrients and a better growth environment for wheat.

[0021] The beneficial effects of the present invention are as follows: the present invention adopts three strains to work synergistically to achieve the effect of promoting wheat growth, improving wheat yield and quality, and can also effectively reduce the occurrence of diseases; the plant extract used in the present invention contains monk fruit seed extract and sodium N-coconut oil acyl-N-methyl taurate, and the two work together to maintain the high activity and long-term stability of the composite microbial agent; after being applied to the soil, the highly active microorganisms in the composite microbial solution can secrete a variety of plant growth regulators, such as chitinase, cellulase, etc., which can promote the growth and development of wheat; and the various active ingredients in the monk fruit seed extract also provide additional energy and nutrient support for wheat, and work together with beneficial microorganisms to enhance the stress resistance of wheat plants and reduce the occurrence of diseases. At the same time, the active ingredients in the plant extract will affect the metabolic process of wheat, thereby improving the quality of wheat. The components of the composite microbial agent prepared by the present invention work synergistically, can improve the soil environment, promote nutrient absorption and utilization, and increase the growth rate and stress resistance of wheat, thereby increasing yield and improving quality. At the same time, the present invention also has the advantages of environmental friendliness and sustainability, and provides new ideas and methods for the green development of agriculture. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 The results of the SOD activity test in wheat leaves of different treatment groups of the present invention; Figure 2 The CAT activity test results in wheat leaves of different treatment groups of the present invention; Figure 3 These are the test results of POD activity in wheat leaves of different treatment groups of the present invention. DETAILED DESCRIPTION

[0023] The technical solution of the present invention is further described below in conjunction with specific embodiments, but is not limited thereto. The Streptomyces rutinus, Bacillus nigeri, and Bacillus velez used in the present invention can be purchased through the strain catalog of the preservation center without the need for additional preservation.

[0024] Example 1 A composite microbial agent for promoting wheat yield and income increase is prepared from the following raw materials in parts by weight: 100 kg of microbial agent, 10 kg of nutrient solution, 20 kg of plant extract, and 3 kg of promoter; the microbial agent is prepared from Streptomyces ruticosa, Bacillus niger, and Bacillus velez; the plant extract is composed of monk fruit seed extract and sodium N-cocoyl-N-methyltaurate in a mass ratio of 5:1; the promoter is composed of polyvinyl alcohol and phytic acid in a mass ratio of 2:1.

[0025] The preparation method of the microbial agent is as follows: thawing and activating Streptomyces ruticosa, Bacillus nielsenii and Bacillus velez respectively, culturing in a 28°C incubator for 48 hours after streaking on a plate, taking a loop of the cultured strain in NB liquid culture medium, placing it in a shaker at 30°C and 150 r / min for shaking culture for 24 hours to obtain seed liquid; inoculating the seed liquids of the three strains into 200 mL of TSB liquid culture medium at an inoculation rate of 10%, shaking and culturing at 30°C and 200 r / min for 72 hours to obtain fermentation liquid; mixing the three fermentation liquids in a volume ratio of 1:1:1 to obtain the microbial agent.

[0026] The composition of the NB liquid culture medium used was: 5 g of peptone, 2 g of yeast extract, 3 g of beef extract, 5 g of sodium chloride, and 1000 ml of distilled water; pH 7.0, sterilization conditions: 121°C, 20 min.

[0027] The composition of the TSB liquid culture medium used was: 2.5 g yeast extract, 17 g tryptone, 3 g soy peptone, 5 g sodium chloride, 2.5 g glucose, 2.5 g potassium dihydrogen phosphate, 1000 ml distilled water; pH 7.2, sterilization conditions: 121°C, 20 min.

[0028] The Streptomyces vinifera, strain number CGMCC No.4.7294, was purchased from the China General Microbiological Culture Collection Administration Center, and the preservation date was June 26, 2015; the Bacillus nissoni, strain number CGMCC No.1.16092, was purchased from the China General Microbiological Culture Collection Administration Center, and the preservation date was February 13, 2017; the Bacillus velez, strain number CGMCC No.1.12669, was purchased from the China General Microbiological Culture Collection Administration Center, and the preservation date was September 10, 2013.

[0029] The preparation method of the monk fruit seed extract is as follows: (1) Wash and dry the monk fruit seeds, grind them into powder, and pass them through a 100-mesh sieve to obtain monk fruit seed powder; (2) Add deionized water to the monk fruit seed powder according to the material-liquid ratio, then add a composite enzyme solution containing neutral protease and cellulase, adjust the solution pH to 7.0, perform enzymolysis at 35°C for 6 hours, then inactivate the enzyme at 90°C for 10 minutes, and then cool to room temperature to obtain an enzymolysis solution; (3) Add the same volume of 25% ethanol to the enzymatic hydrolyzate, adjust the pH of the mixed solution to 5.0 with acetic acid, heat to 39°C, extract for 90 min, and centrifuge to obtain the supernatant. Concentrate the supernatant to one third of its original volume to obtain the monk fruit seed extract.

[0030] In the step (2), the material-liquid ratio of monk fruit seed powder to deionized water is 1 g:30 ml.

[0031] The mass volume ratio of the monk fruit seed powder to the complex enzyme solution is 1g:20ml; the neutral protease content in the complex enzyme solution is 20g / L, and the cellulase content is 5g / L.

[0032] The enzyme activity of the neutral protease is 100,000 u / g, and the enzyme activity of the cellulase is 200,000 u / g.

[0033] The nutrient solution is composed of urea, trehalose, amino acid chelated zinc, amino acid chelated magnesium, amino acid chelated iron and deionized water in a mass ratio of 5:1:0.05:0.02:0.1:100.

[0034] The preparation method of the composite microbial agent for promoting wheat production and income increase comprises the following steps: (1) Preparation of microbial agents; (2) preparing a monk fruit seed extract, and then mixing it with sodium N-cocoyl-N-methyltaurate according to a mass ratio to prepare a plant extract; (3) Mix the microbial agent with the plant extract, and then homogenize it in a homogenizer for 5 minutes. Then add the nutrient solution and promoter, and stir evenly to obtain the final product.

[0035] Example 2 A composite microbial agent for promoting wheat yield and income increase is prepared from the following raw materials in parts by weight: 150 kg of microbial agent, 20 kg of nutrient solution, 30 kg of plant extract, and 5 kg of promoter; the microbial agent is prepared from Streptomyces ruticosa, Bacillus niger, and Bacillus velezii; the plant extract is composed of monk fruit seed extract and sodium N-cocoyl-N-methyltaurate in a mass ratio of 5:1; the promoter is composed of polyvinyl alcohol and phytic acid in a mass ratio of 2:1.

[0036] The preparation method of the microbial agent is as follows: thawing and activating Streptomyces ruticosa, Bacillus nielsenii and Bacillus velez respectively, culturing in a 28°C incubator for 48 hours after streaking on a plate, taking a loop of the cultured strain in NB liquid culture medium, placing it in a shaker at 30°C and 150 r / min for shaking culture for 24 hours to obtain seed liquid; inoculating the seed liquids of the three strains into 200 mL of TSB liquid culture medium at an inoculation rate of 10%, shaking and culturing at 30°C and 200 r / min for 72 hours to obtain fermentation liquid; mixing the three fermentation liquids in a volume ratio of 1:1:1 to obtain the microbial agent.

[0037] The composition of the NB liquid culture medium used was: 5 g of peptone, 2 g of yeast extract, 3 g of beef extract, 5 g of sodium chloride, and 1000 ml of distilled water; pH 7.0, sterilization conditions: 121°C, 20 min.

[0038] The composition of the TSB liquid culture medium used was: 2.5 g yeast extract, 17 g tryptone, 3 g soy peptone, 5 g sodium chloride, 2.5 g glucose, 2.5 g potassium dihydrogen phosphate, 1000 ml distilled water; pH 7.2, sterilization conditions: 121°C, 20 min.

[0039] The Streptomyces vinifera, strain number CGMCC No.4.7294, was purchased from the China General Microbiological Culture Collection Administration Center, and the preservation date was June 26, 2015; the Bacillus nissoni, strain number CGMCC No.1.16092, was purchased from the China General Microbiological Culture Collection Administration Center, and the preservation date was February 13, 2017; the Bacillus velez, strain number CGMCC No.1.12669, was purchased from the China General Microbiological Culture Collection Administration Center, and the preservation date was September 10, 2013.

[0040] The preparation method of the monk fruit seed extract is as follows: (1) Wash and dry the monk fruit seeds, grind them into powder, and pass them through a 100-mesh sieve to obtain monk fruit seed powder; (2) adding deionized water to the monk fruit seed powder according to the material-liquid ratio, and then adding a composite enzyme solution containing neutral protease and cellulase thereto, adjusting the solution pH to 7.0, performing enzymolysis at 35°C for 8 hours, and then inactivating the enzyme at 90°C for 10 minutes and cooling to room temperature to obtain an enzymolysis solution; (3) Add the same volume of 25% ethanol to the enzymatic hydrolyzate, adjust the pH of the mixed solution to 5.5 with acetic acid, heat to 39°C, extract for 120 min, centrifuge and separate the supernatant. Concentrate the supernatant to one third of its original volume to obtain the monk fruit seed extract.

[0041] In the step (2), the material-liquid ratio of monk fruit seed powder to deionized water is 1 g:30 ml.

[0042] The mass volume ratio of the monk fruit seed powder to the complex enzyme solution is 1g:20ml; the neutral protease content in the complex enzyme solution is 30g / L, and the cellulase content is 10g / L.

[0043] The enzyme activity of the neutral protease is 100,000 u / g, and the enzyme activity of the cellulase is 200,000 u / g.

[0044] The nutrient solution is composed of urea, trehalose, amino acid chelated zinc, amino acid chelated magnesium, amino acid chelated iron and deionized water in a mass ratio of 7:2:0.05:0.02:0.1:100.

[0045] The preparation method of the composite microbial agent for promoting wheat production and income increase comprises the following steps: (1) Preparation of microbial agents; (2) preparing a monk fruit seed extract, and then mixing it with sodium N-cocoyl-N-methyltaurate according to a mass ratio to prepare a plant extract; (3) Mix the microbial agent with the plant extract, and then homogenize it in a homogenizer for 10 minutes. Then add the nutrient solution and promoter, and stir evenly to obtain the final product.

[0046] Example 3 A composite microbial agent for promoting wheat yield and income increase, which is prepared from the following raw materials in parts by weight: 120kg of microbial agent, 15kg of nutrient solution, 25kg of plant extract, and 4kg of promoter; the microbial agent is prepared from Streptomyces ruticosa, Bacillus niger, and Bacillus velezii; the plant extract is composed of monk fruit seed extract and sodium N-cocoyl-N-methyltaurate in a mass ratio of 5:1; the promoter is composed of polyvinyl alcohol and phytic acid in a mass ratio of 2:1.

[0047] The preparation method of the microbial agent is as follows: thawing and activating Streptomyces ruticosa, Bacillus nielsenii and Bacillus velez respectively, culturing in a 28°C incubator for 48 hours after streaking on a plate, taking a loop of the cultured strain in NB liquid culture medium, placing it in a shaker at 30°C and 150 r / min for shaking culture for 24 hours to obtain seed liquid; inoculating the seed liquids of the three strains into 200 mL of TSB liquid culture medium at an inoculation rate of 10%, shaking and culturing at 30°C and 200 r / min for 72 hours to obtain fermentation liquid; mixing the three fermentation liquids in a volume ratio of 1:1:1 to obtain the microbial agent.

[0048] The composition of the NB liquid culture medium used was: 5 g of peptone, 2 g of yeast extract, 3 g of beef extract, 5 g of sodium chloride, and 1000 ml of distilled water; pH 7.0, sterilization conditions: 121°C, 20 min.

[0049] The composition of the TSB liquid culture medium used was: 2.5 g yeast extract, 17 g tryptone, 3 g soy peptone, 5 g sodium chloride, 2.5 g glucose, 2.5 g potassium dihydrogen phosphate, 1000 ml distilled water; pH 7.2, sterilization conditions: 121°C, 20 min.

[0050] The Streptomyces vinifera, strain number CGMCC No.4.7294, was purchased from the China General Microbiological Culture Collection Administration Center, and the preservation date was June 26, 2015; the Bacillus nissoni, strain number CGMCC No.1.16092, was purchased from the China General Microbiological Culture Collection Administration Center, and the preservation date was February 13, 2017; the Bacillus velez, strain number CGMCC No.1.12669, was purchased from the China General Microbiological Culture Collection Administration Center, and the preservation date was September 10, 2013.

[0051] The preparation method of the monk fruit seed extract is as follows: (1) Wash and dry the monk fruit seeds, grind them into powder, and pass them through a 100-mesh sieve to obtain monk fruit seed powder; (2) adding deionized water to the monk fruit seed powder according to the material-liquid ratio, and then adding a composite enzyme solution containing neutral protease and cellulase thereto, adjusting the solution pH to 7.0, performing enzymolysis at 35°C for 7 hours, and then inactivating the enzyme at 90°C for 10 minutes and cooling to room temperature to obtain an enzymolysis solution; (3) Add the same volume of 25% ethanol to the enzymatic hydrolyzate, adjust the pH of the mixed solution to 5.3 with acetic acid, heat to 39°C, extract for 100 min, centrifuge and separate the supernatant. Concentrate the supernatant to one third of its original volume to obtain the monk fruit seed extract.

[0052] In the step (2), the material-liquid ratio of monk fruit seed powder to deionized water is 1 g:30 ml.

[0053] The mass volume ratio of the monk fruit seed powder to the complex enzyme solution is 1g:20ml; the neutral protease content in the complex enzyme solution is 30g / L, and the cellulase content is 10g / L.

[0054] The enzyme activity of the neutral protease is 100,000 u / g, and the enzyme activity of the cellulase is 200,000 u / g.

[0055] The nutrient solution is composed of urea, trehalose, amino acid chelated zinc, amino acid chelated magnesium, amino acid chelated iron and deionized water in a mass ratio of 6:1:0.05:0.02:0.1:100.

[0056] The preparation method of the composite microbial agent for promoting wheat production and income increase comprises the following steps: (1) Preparation of microbial agents; (2) preparing a monk fruit seed extract, and then mixing it with sodium N-cocoyl-N-methyltaurate according to a mass ratio to prepare a plant extract; (3) Mix the microbial agent with the plant extract, and then homogenize it in a homogenizer for 10 minutes. Then add the nutrient solution and promoter, and stir evenly to obtain the final product.

[0057] Comparative Example 1 A composite microbial agent for promoting increased production and income of wheat is prepared from the following raw materials in parts by weight: 120 kg of microbial agent, 15 kg of nutrient solution, 25 kg of plant extract, and 4 kg of promoter; the microbial agent is prepared from Bacillus nissii and Bacillus velezii; the plant extract is composed of monk fruit seed extract and sodium N-coconut oil acyl-N-methyl taurate in a mass ratio of 5:1; the promoter is composed of polyvinyl alcohol and phytic acid in a mass ratio of 2:1.

[0058] The preparation method of the microbial agent is as follows: Bacillus nissoni and Bacillus velez are thawed and activated respectively, and the plates are streaked and cultured in a 28°C incubator for 48 hours, a loop of the cultured strain is taken into NB liquid culture medium, and the culture is placed in a shaker at 30°C and 150 r / min for 24 hours to obtain seed liquid; the seed liquids of the two strains are inoculated into 200 mL of TSB liquid culture medium at an inoculation rate of 10%, and the culture is shaken at 30°C and 200 r / min for 72 hours to obtain fermentation liquid; the two fermentation liquids are mixed in a volume ratio of 1:1 to obtain the microbial agent.

[0059] The composition of the NB liquid culture medium used was: 5 g of peptone, 2 g of yeast extract, 3 g of beef extract, 5 g of sodium chloride, and 1000 ml of distilled water; pH 7.0, sterilization conditions: 121°C, 20 min.

[0060] The composition of the TSB liquid culture medium used was: 2.5 g yeast extract, 17 g tryptone, 3 g soy peptone, 5 g sodium chloride, 2.5 g glucose, 2.5 g potassium dihydrogen phosphate, 1000 ml distilled water; pH 7.2, sterilization conditions: 121°C, 20 min.

[0061] The Bacillus nissoni, strain number CGMCC No.1.16092, was purchased from the China General Microbiological Culture Collection Administration Center, and the preservation date was February 13, 2017; the Bacillus velez, strain number CGMCC No.1.12669, was purchased from the China General Microbiological Culture Collection Administration Center, and the preservation date was September 10, 2013.

[0062] The preparation method of the monk fruit seed extract is as follows: (1) Wash and dry the monk fruit seeds, grind them into powder, and pass them through a 100-mesh sieve to obtain monk fruit seed powder; (2) adding deionized water to the monk fruit seed powder according to the material-liquid ratio, and then adding a composite enzyme solution containing neutral protease and cellulase thereto, adjusting the solution pH to 7.0, performing enzymolysis at 35°C for 7 hours, and then inactivating the enzyme at 90°C for 10 minutes and cooling to room temperature to obtain an enzymolysis solution; (3) Add the same volume of 25% ethanol to the enzymatic hydrolyzate, adjust the pH of the mixed solution to 5.3 with acetic acid, heat to 39°C, extract for 100 min, centrifuge and separate the supernatant. Concentrate the supernatant to one third of its original volume to obtain the monk fruit seed extract.

[0063] In the step (2), the material-liquid ratio of monk fruit seed powder to deionized water is 1 g:30 ml.

[0064] The mass volume ratio of the monk fruit seed powder to the complex enzyme solution is 1g:20ml; the neutral protease content in the complex enzyme solution is 30g / L, and the cellulase content is 10g / L.

[0065] The enzyme activity of the neutral protease is 100,000 u / g, and the enzyme activity of the cellulase is 200,000 u / g.

[0066] The nutrient solution is composed of urea, trehalose, amino acid chelated zinc, amino acid chelated magnesium, amino acid chelated iron and deionized water in a mass ratio of 6:1:0.05:0.02:0.1:100.

[0067] The preparation method of the composite microbial agent for promoting wheat production and income increase comprises the following steps: (1) Preparation of microbial agents; (2) preparing a monk fruit seed extract, and then mixing it with sodium N-cocoyl-N-methyltaurate according to a mass ratio to prepare a plant extract; (3) Mix the microbial agent with the plant extract, and then homogenize it in a homogenizer for 10 minutes. Then add the nutrient solution and promoter, and stir evenly to obtain the final product.

[0068] This comparative example is basically the same as Example 3, the only difference being that the microbial agent does not contain Streptomyces ruticosa.

[0069] Comparative Example 2 A composite microbial agent for promoting wheat yield and income increase, which is prepared from the following raw materials in parts by weight: 120 kg of microbial agent, 15 kg of nutrient solution, 25 kg of plant extract, and 4 kg of promoter; the microbial agent is prepared from Streptomyces ruticosa and Bacillus velez; the plant extract is composed of monk fruit seed extract and sodium N-cocoyl-N-methyltaurate in a mass ratio of 5:1; the promoter is composed of polyvinyl alcohol and phytic acid in a mass ratio of 2:1.

[0070] The preparation method of the microbial agent is as follows: thawing and activating Streptomyces ruticosa and Bacillus velezii respectively, culturing in a 28°C incubator for 48 hours after streaking a plate, taking a loop of the cultured strain in NB liquid culture medium, placing it in a shaker at 30°C and 150 r / min for shaking culture for 24 hours to obtain seed liquid; inoculating the seed liquids of the two strains into 200 mL of TSB liquid culture medium at an inoculation rate of 10%, shaking and culturing at 30°C and 200 r / min for 72 hours to obtain fermentation liquid; mixing the two fermentation liquids in a volume ratio of 1:1 to obtain the microbial agent.

[0071] The composition of the NB liquid culture medium used was: 5 g of peptone, 2 g of yeast extract, 3 g of beef extract, 5 g of sodium chloride, and 1000 ml of distilled water; pH 7.0, sterilization conditions: 121°C, 20 min.

[0072] The composition of the TSB liquid culture medium used was: 2.5 g yeast extract, 17 g tryptone, 3 g soy peptone, 5 g sodium chloride, 2.5 g glucose, 2.5 g potassium dihydrogen phosphate, 1000 ml distilled water; pH 7.2, sterilization conditions: 121°C, 20 min.

[0073] The Streptomyces vinifera, strain number CGMCC No.4.7294, was purchased from the China General Microbiological Culture Collection Administration Center, and the preservation date was June 26, 2015; the Bacillus velez, strain number CGMCC No.1.12669, was purchased from the China General Microbiological Culture Collection Administration Center, and the preservation date was September 10, 2013.

[0074] The preparation method of the monk fruit seed extract is as follows: (1) Wash and dry the monk fruit seeds, grind them into powder, and pass them through a 100-mesh sieve to obtain monk fruit seed powder; (2) adding deionized water to the monk fruit seed powder according to the material-liquid ratio, and then adding a composite enzyme solution containing neutral protease and cellulase thereto, adjusting the solution pH to 7.0, performing enzymolysis at 35°C for 7 hours, and then inactivating the enzyme at 90°C for 10 minutes and cooling to room temperature to obtain an enzymolysis solution; (3) Add the same volume of 25% ethanol to the enzymatic hydrolyzate, adjust the pH of the mixed solution to 5.3 with acetic acid, heat to 39°C, extract for 100 min, centrifuge and separate the supernatant. Concentrate the supernatant to one third of its original volume to obtain the monk fruit seed extract.

[0075] In the step (2), the material-liquid ratio of monk fruit seed powder to deionized water is 1 g:30 ml.

[0076] The mass volume ratio of the monk fruit seed powder to the complex enzyme solution is 1g:20ml; the neutral protease content in the complex enzyme solution is 30g / L, and the cellulase content is 10g / L.

[0077] The enzyme activity of the neutral protease is 100,000 u / g, and the enzyme activity of the cellulase is 200,000 u / g.

[0078] The nutrient solution is composed of urea, trehalose, amino acid chelated zinc, amino acid chelated magnesium, amino acid chelated iron and deionized water in a mass ratio of 6:1:0.05:0.02:0.1:100.

[0079] The preparation method of the composite microbial agent for promoting wheat production and income increase comprises the following steps: (1) Preparation of microbial agents; (2) preparing a monk fruit seed extract, and then mixing it with sodium N-cocoyl-N-methyltaurate according to a mass ratio to prepare a plant extract; (3) Mix the microbial agent with the plant extract, and then homogenize it in a homogenizer for 10 minutes. Then add the nutrient solution and promoter, and stir evenly to obtain the final product.

[0080] This comparative example is basically the same as Example 3, except that the microbial agent does not contain Bacillus niger.

[0081] Comparative Example 3 A composite microbial agent for promoting wheat yield and income increase, which is prepared from the following raw materials in parts by weight: 120kg of microbial agent, 15kg of nutrient solution, 25kg of plant extract, and 4kg of promoter; the microbial agent is prepared from Streptomyces ruticosa and Bacillus niger; the plant extract is composed of monk fruit seed extract and sodium N-cocoyl-N-methyltaurate in a mass ratio of 5:1; the promoter is composed of polyvinyl alcohol and phytic acid in a mass ratio of 2:1.

[0082] The preparation method of the microbial agent is as follows: thawing and activating Streptomyces vinifera and Bacillus nissoni respectively, culturing in a 28°C incubator for 48 hours after streaking on a plate, taking a loop of the cultured strain in NB liquid culture medium, placing it in a shaker at 30°C and 150 r / min for shaking culture for 24 hours to obtain seed liquid; inoculating the seed liquids of the two strains into 200 mL of TSB liquid culture medium at an inoculation rate of 10%, shaking and culturing at 30°C and 200 r / min for 72 hours to obtain fermentation liquid; mixing the two fermentation liquids in a volume ratio of 1:1 to obtain the microbial agent.

[0083] The composition of the NB liquid culture medium used was: 5 g of peptone, 2 g of yeast extract, 3 g of beef extract, 5 g of sodium chloride, and 1000 ml of distilled water; pH 7.0, sterilization conditions: 121°C, 20 min.

[0084] The composition of the TSB liquid culture medium used was: 2.5 g yeast extract, 17 g tryptone, 3 g soy peptone, 5 g sodium chloride, 2.5 g glucose, 2.5 g potassium dihydrogen phosphate, 1000 ml distilled water; pH 7.2, sterilization conditions: 121°C, 20 min.

[0085] The Streptomyces vinifera, strain number CGMCC No.4.7294, was purchased from the China General Microbiological Culture Collection Administration Center, and the preservation date was June 26, 2015; the Bacillus nissoni, strain number CGMCC No.1.16092, was purchased from the China General Microbiological Culture Collection Administration Center, and the preservation date was February 13, 2017; the Bacillus velez, strain number CGMCC No.1.12669, was purchased from the China General Microbiological Culture Collection Administration Center, and the preservation date was September 10, 2013.

[0086] The preparation method of the monk fruit seed extract is as follows: (1) Wash and dry the monk fruit seeds, grind them into powder, and pass them through a 100-mesh sieve to obtain monk fruit seed powder; (2) adding deionized water to the monk fruit seed powder according to the material-liquid ratio, and then adding a composite enzyme solution containing neutral protease and cellulase thereto, adjusting the solution pH to 7.0, performing enzymolysis at 35°C for 7 hours, and then inactivating the enzyme at 90°C for 10 minutes and cooling to room temperature to obtain an enzymolysis solution; (3) Add the same volume of 25% ethanol to the enzymatic hydrolyzate, adjust the pH of the mixed solution to 5.3 with acetic acid, heat to 39°C, extract for 100 min, centrifuge and separate the supernatant. Concentrate the supernatant to one third of its original volume to obtain the monk fruit seed extract.

[0087] In the step (2), the material-liquid ratio of monk fruit seed powder to deionized water is 1 g:30 ml.

[0088] The mass volume ratio of the monk fruit seed powder to the complex enzyme solution is 1g:20ml; the neutral protease content in the complex enzyme solution is 30g / L, and the cellulase content is 10g / L.

[0089] The enzyme activity of the neutral protease is 100,000 u / g, and the enzyme activity of the cellulase is 200,000 u / g.

[0090] The nutrient solution is composed of urea, trehalose, amino acid chelated zinc, amino acid chelated magnesium, amino acid chelated iron and deionized water in a mass ratio of 6:1:0.05:0.02:0.1:100.

[0091] The preparation method of the composite microbial agent for promoting wheat production and income increase comprises the following steps: (1) Preparation of microbial agents; (2) preparing a monk fruit seed extract, and then mixing it with sodium N-cocoyl-N-methyltaurate according to a mass ratio to prepare a plant extract; (3) Mix the microbial agent with the plant extract, and then homogenize it in a homogenizer for 10 minutes. Then add the nutrient solution and promoter, and stir evenly to obtain the final product.

[0092] This comparative example is basically the same as Example 3, except that the microbial agent does not contain Bacillus Velezii.

[0093] Comparative Example 4 This comparative example is basically the same as Example 3, except that the three fermentation broths in the microbial inoculant are mixed in a volume ratio of 2:1:1.

[0094] Comparative Example 5 This comparative example is basically the same as Example 3, except that it does not contain plant extract.

[0095] Comparative Example 6 This comparative example is basically the same as Example 3, except that the plant extract is Momordica grosvenori seed extract.

[0096] Comparative Example 7 This comparative example is basically the same as Example 3, except that the plant extract is sodium N-cocoyl-N-methyltaurate.

[0097] Test Example 1 The long-term stability of the composite microbial agent products for promoting wheat yield and income increase prepared in Examples 1-3 and Comparative Examples 1-7 was tested, and the effective viable count was determined according to the method in NY / T 2321-2013 "Microbial Fertilizer Product Inspection Procedure". Specific test results are shown in Table 1.

[0098] Table 1 Test results It can be seen from the data in Table 1 above that, compared with the comparative example, the composite microbial agent prepared in the embodiment of the present invention has better long-term stability. This is because the active ingredients in the plant extract of the present invention can provide abundant energy for the microorganisms, which helps to maintain the activity of the microorganisms. On the other hand, the active ingredients therein also have an antioxidant effect, which can remove free radicals and protect microbial cells from oxidative damage, which helps to prolong the survival time of the microbial agent, thereby maintaining the long-term stability of the effective bacteria.

[0099] Application Examples Effect of wheat planting experiment in the field The cultivated land in Yanjiabeilou Village, Shangkou Town, Shouguang City, Shandong Province was selected as the wheat planting test site. The soil physical and chemical properties are shown in Table 2.

[0100] Table 2 Basic physical and chemical properties of soil The wheat variety tested was Shannong 27. There were 11 treatments in total, each with 3 replicates and the average value. The area of ​​each plot was 100m 2 The specific test method is: before wheat sowing, conventional compound fertilizer (N: P2O5: K2O = 20: 15: 15) is used as base fertilizer and turned into the soil, with an amount of 20 kg / mu; after wheat seedlings emerge, the composite microbial agent prepared by Examples 1-3 and Comparative Examples 1-7 of the present invention is sprayed at the seedling stage, the composite microbial agent is diluted with 50 times the volume of clean water, stirred evenly and sprayed, and the amount of the composite microbial agent stock solution per mu is 1 L; a blank comparative ratio is set for the test, the blank comparative ratio only uses base fertilizer and no microbial agent is applied, and the other management measures of each treatment group are conventional wheat field management measures.

[0101] Take fresh wheat flag leaves at the flowering stage from each treatment group (using the five-point sampling method, 10 flag leaves for each sampling point), rinse and wipe off impurities on the tissue surface, cut into pieces (remove the midrib), and mix well; weigh 0.2 g of the cut fresh sample, a total of 3 portions, put it in a mortar, add a small amount of quartz sand and calcium carbonate powder and 2-3 mL of 95% ethanol, grind it into a homogenous slurry, add 10 mL of ethanol, and continue to grind until the tissue turns white. Let it stand for 3 to 5 minutes; take a piece of filter paper, put it in a funnel, moisten it with ethanol, pour the extract into the funnel along the glass rod, filter it into a 25 mL brown volumetric flask, rinse the mortar, pestle and residue several times with a small amount of ethanol, and finally pour it into the funnel together with the residue; use a dropper to absorb ethanol and wash all the chloroplast pigments on the filter paper into the volumetric flask. Until there is no green in the filter paper and the residue. Finally, dilute to 25 mL with ethanol and shake well; pour the chloroplast pigment extract into a colorimetric cup with a diameter of 1 cm. The total chlorophyll content of each treatment group was determined by spectrophotometry. The specific results are shown in Table 3.

[0102] Table 3 Total chlorophyll content of wheat leaves in different treatment groups (mg / L) From the results in Table 3 above, it can be seen that compared with the blank control group, the total chlorophyll content increased by more than 28.6%, which shows that the composite microbial agent of the present invention promoted photosynthesis after being used for wheat planting. The enhancement of photosynthesis can increase the photosynthetic rate of wheat leaves, increase the accumulation of photosynthetic products, provide necessary energy and nutrients for wheat, and promote the growth and development of wheat.

[0103] Three random points were selected for each treatment, and 10 wheat plants were selected from each point. The number of tillers per plant, the number of secondary roots, and the dry matter mass per plant were investigated during the wintering period. The plant height, base stem diameter, and flag leaf area were investigated during the grain filling period. The specific results are shown in Table 4.

[0104] Table 4 Survey results of wheat growth in different treatment groups It can be seen from the contents of Table 4 above that, compared with the control group, the composite microbial agent prepared in the embodiment of the present invention has significant growth-promoting effects on wheat at different growth stages. The number of tillers per plant, the number of secondary roots, and the dry matter mass per plant during the wintering period are significantly higher than those of the blank control group and comparative examples 1-7; the plant height, stem diameter, and flag leaf area during the filling period are also significantly higher than those of the comparative example and the control group. This shows that after using the composite microbial agent prepared in the embodiment of the present invention, the growth-promoting effects on different growth cycles of wheat are more obvious, and after changing the composition and dosage of the present invention, the growth-promoting effect disappears or weakens accordingly.

[0105] At the harvest period, the total weight of wheat yield in different treatment groups was counted and converted into per mu yield according to the area. At the same time, the wheat quality of different treatment groups was also studied. The specific research method was: the wheat ears were harvested by five-point sampling method, repeated 3 times, fine threshing and air drying were carried out, and the thousand-grain weight was measured. The harvested wheat grains were crushed and passed through an 80-mesh sieve. The wheat protein content was measured according to the Kjeldahl nitrogen determination method in GB5009.5-2016, and the wet gluten content was determined according to GB / T 5506.1-2008. The statistical results are shown in Table 5.

[0106] Table 5 Wheat yield and quality in different treatment groups Superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in wheat are important components of the antioxidant enzyme system. They can remove reactive oxygen species (ROS) produced by stress, thereby protecting wheat cells from oxidative damage. The present invention also measured the activity of defense enzymes in wheat leaves in different treatment groups. The specific operation method is: weigh 0.1 g of wheat leaves and add 3 ml PBS (pH 7.8) first, grind and homogenize in an ice bath, then add 2 mL PBS to rinse the mortar, mix well, pour into a centrifuge tube and centrifuge at 10000r / min at 4°C for 20 min. The supernatant is the crude enzyme solution, which can be used for enzyme activity determination. The contents of SOD, CAT and POD in the crude enzyme extract were determined respectively, among which SOD was determined by the nitroblue tetrazolium photoreduction method; CAT was determined by the ultraviolet absorption method, and POD was determined by the guaiacol method. The specific results are as follows: Figure 1-3 shown.

[0107] from Figure 1-3 It can be seen from the data that after the composite microbial agent for promoting wheat production and income increase prepared by the embodiment of the present invention is used, the activities of the three enzymes of wheat are significantly higher than those of the blank control group and the comparative example, which shows that the stress resistance of wheat is enhanced and can better cope with various stress conditions. Under stress conditions, highly active defense enzymes can help wheat plants better adapt to and resist these stresses; highly active defense enzymes can not only enhance the stress resistance of wheat and reduce the infection of pests and diseases, but also promote the growth and development of wheat, and provide more growth space and opportunities for wheat by protecting wheat cells from stress damage, thereby improving the yield and quality of wheat.

[0108] It should be noted that the above embodiments are only partial embodiments of the preferred methods of implementing the present invention, rather than all embodiments. Obviously, based on the above embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

Claims

1. A composite microbial agent for increasing wheat production and income, characterized in that: The invention is prepared from the following raw materials in parts by weight: 100-150 parts of microbial agent, 10-20 parts of nutrient solution, 20-30 parts of plant extract, and 3-5 parts of promoter; the microbial agent is Streptomyces vinifera ( Streptomyces vinaceusdrappus )、Bacillus niger( Bacillus nealsonii ), Bacillus Velez Bacillus velezensis ); the plant extract is composed of monk fruit seed extract and sodium N-cocoyl-N-methyl taurate in a mass ratio of 5:1; the accelerator is composed of polyvinyl alcohol and phytic acid in a mass ratio of 2:

1.

2. The composite microbial agent for promoting wheat production and income increase according to claim 1, characterized in that: The preparation method of the microbial agent is as follows: thawing and activating Streptomyces ruthenica, Bacillus niger and Bacillus velez respectively, culturing in a 28°C incubator for 48 hours after streaking on a plate, taking a loop of the cultured strain in NB liquid culture medium, placing it in a shaking incubator at 30°C and 150 r / min for 24 hours to obtain a seed solution; The seed liquid of the three strains was inoculated into 200 mL of TSB liquid culture medium at an inoculation rate of 10%, and cultured at 30°C and 200 r / min for 72 h to obtain fermentation liquid; the three fermentation liquids were mixed in a volume ratio of 1:1:1 to obtain a microbial agent.

3. The composite microbial agent for promoting wheat production and income increase according to claim 2, characterized in that: The Streptomyces ruticosa has a strain number of CGMCC No.4.7294 and a preservation date of June 26, 2015, and the preservation unit is the General Microbiology Center of the China National Microorganism Culture Collection Administration; the Bacillus nissoni has a strain number of CGMCC No.1.16092 and a preservation date of February 13, 2017, and the preservation unit is the General Microbiology Center of the China National Microorganism Culture Collection Administration; the Bacillus velez has a strain number of CGMCC No.1.12669 and a preservation date of September 10, 2013, and the preservation unit is the General Microbiology Center of the China National Microorganism Culture Collection Administration.

4. The composite microbial agent for promoting wheat production and income increase according to claim 1, characterized in that: The preparation method of the monk fruit seed extract is as follows: (1) Wash and dry the monk fruit seeds, grind them into powder, and pass them through a 100-mesh sieve to obtain monk fruit seed powder; (2) adding deionized water to the monk fruit seed powder according to the material-liquid ratio, and then adding a composite enzyme solution containing neutral protease and cellulase thereto, adjusting the solution pH to 7.0, performing enzymolysis at 35°C for 6-8h, then inactivating the enzyme at 90°C for 10min, and cooling to room temperature to obtain an enzymolysis solution; (3) Add an equal volume of 25% ethanol to the enzymatic hydrolyzate, adjust the pH of the mixed solution to 5.0-5.5 with acetic acid, heat to 39°C, extract for 90-120 min, centrifuge to obtain the supernatant, and concentrate the supernatant to one third of the original volume to obtain the monk fruit seed extract.

5. The composite microbial agent for promoting wheat production and income increase according to claim 4, characterized in that: In the step (2), the material-liquid ratio of monk fruit seed powder to deionized water is 1 g:30 ml.

6. The composite microbial agent for promoting wheat production and income increase according to claim 4, characterized in that: The mass volume ratio of the monk fruit seed powder to the complex enzyme solution is 1g:20ml; the neutral protease content in the complex enzyme solution is 20-30g / L, and the cellulase content is 5-10g / L.

7. The composite microbial agent for promoting wheat production and income increase according to claim 6, characterized in that: The enzyme activity of the neutral protease is 100,000 u / g, and the enzyme activity of the cellulase is 200,000 u / g.

8. The composite microbial agent for increasing wheat production and income according to claim 1, characterized in that: The nutrient solution is composed of urea, trehalose, amino acid chelated zinc, amino acid chelated magnesium, amino acid chelated iron and deionized water in a mass ratio of 5-7:1-2:0.05:0.02:0.1:

100.

9. A method for preparing the composite microbial agent for increasing wheat production and income according to any one of claims 1 to 9, characterized in that: It includes the following steps: (1) Preparation of microbial agents; (2) preparing a monk fruit seed extract, and then mixing it with sodium N-cocoyl-N-methyltaurate according to a mass ratio to prepare a plant extract; (3) Mix the microbial agent with the plant extract, and then homogenize it in a homogenizer for 5-10 minutes, then add the nutrient solution and promoter, and stir evenly to obtain the final product.

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