Chemiluminescent Immunoassay Analyzer

Abstract

The present invention discloses a chemiluminescent immunoassay analyzer and an automatic control method, wherein the chemiluminescent immunoassay analyzer comprises: a three-dimensional arm assembly, wherein the three-dimensional arm assembly comprises an X-axis guide rail mounted on the main frame, a Y-axis guide rail movably mounted on the X-axis guide rail, and a first Z-axis guide rail and a second Z-axis guide rail movably mounted on the Y-axis guide rail; a gripper, wherein the gripper is movably mounted on the first Z-axis guide rail along the Z-axis direction, and moves within a preset range under the drive of the three-dimensional arm assembly to grab or release a reaction tube in a sample bin to an incubation oscillation mixing assembly; and a syringe pump, wherein the syringe pump is movably mounted on the second Z-axis guide rail along the Z-axis direction, and moves within a preset range under the drive of the three-dimensional arm assembly. The continuous processing of a single sample is realized, thereby realizing assembly line operation and improving analysis efficiency.

Description

Technical Field

[0001] The invention relates to the technical field of chemiluminescence detection, and in particular to a chemiluminescence immunoassay analyzer. Background Art

[0002] Chemiluminescent labeling immunoassay is also called chemiluminescent immunoassay. The chemiluminescent immunoassay instrument consists of two parts, namely the immunoreaction system and the chemiluminescent analysis system. The chemiluminescent analysis system uses the catalysis of the chemiluminescent substance and the oxidation of the oxidant to form an excited intermediate. When this excited intermediate returns to a stable ground state, it emits photons at the same time, and the light quantum yield is measured using a luminescent signal measuring instrument. The immunoreaction system directly labels the luminescent substance on the antigen or antibody, or the enzyme acts on the luminescent substrate.

[0003] In the prior art, single-person chemiluminescence immunoassay analyzers are mostly used for batch processing or single sample experiments. The number of consumables corresponds one-to-one to the number of experiments, and reactants and consumables need to be added repeatedly. There is a lot of human participation in the batch processing process, and assembly line operation cannot be achieved, which affects the analysis efficiency. Summary of the invention

[0004] To this end, the embodiments of the present invention provide a chemiluminescent immunoassay analyzer and an automatic control method, so as to achieve continuous processing of single-person samples, thereby realizing assembly line operation and improving analysis efficiency.

[0005] In order to achieve the above purpose, the embodiment of the present invention provides the following technical solutions:

[0006] The present invention provides a chemiluminescent immunoassay analyzer, comprising:

[0007] A main frame, wherein the bottom of the main frame has a reagent temporary storage area;

[0008] A reagent chamber assembly, wherein a reagent strip is stored in the reagent chamber assembly, and the reagent chamber assembly is mounted on the main frame;

[0009] A magnetic separation component, which is mounted on the main frame and contacts or separates from the reaction tube to drive the reaction tube to shake;

[0010] A consumables library component, which is installed on the main frame and arranged at the lower layer of the reagent compartment component, and contains reaction tubes and TIP heads;

[0011] A sample chamber assembly, in which samples are stored, and the sample chamber assembly is installed on the main frame;

[0012] An incubation and shaking mixing component, which is a temporary storage area for adding samples to the reaction tubes;

[0013] A photometric component, wherein the photometric component has a photometric position and a detection module. After the reaction tube is placed in the photometric position, the photometric data is read by the detection module;

[0014] A three-dimensional arm assembly, the three-dimensional arm assembly comprising an X-axis guide rail mounted on the main frame, a Y-axis guide rail movably mounted on the X-axis guide rail, and a first Z-axis guide rail and a second Z-axis guide rail movably mounted on the Y-axis guide rail;

[0015] A gripper, which is movably mounted on the first Z-axis guide rail along the Z-axis direction, and moves between the waste box discarding position and the magnetic separation component under the drive of the three-dimensional arm component to grab or release the reaction tube in the sample compartment to the photometric component, the incubation and oscillation mixing component, and the magnetic separation component, and grab the reaction tube from the consumables library;

[0016] An injection pump is movably mounted on the second Z-axis guide rail along the Z-axis direction and moves within a preset range under the drive of the three-dimensional arm assembly.

[0017] In some embodiments, the reagent chamber assembly comprises:

[0018] A support frame, the support frame is installed on the main frame;

[0019] A reagent kit, the reagent kit having a plurality of reagent strip placement positions, the reagent kit being installed above the support frame;

[0020] A shuttle assembly, which is movably mounted on the support frame longitudinally and is located below the test kit;

[0021] The shuttle vehicle motion motor is in driving connection with the shuttle vehicle assembly and drives the shuttle vehicle assembly to move linearly to correspond to different reagent strip placement positions.

[0022] In some embodiments, the shuttle assembly includes:

[0023] A vehicle frame, the vehicle frame being drivingly connected to the shuttle vehicle motion motor;

[0024] A push plate, the push plate is mounted on the frame and moves with the frame;

[0025] A push plate motor, the push plate motor is drivingly connected to the push plate and drives the push plate to reciprocate relative to the frame to push out the reagent strip in the reagent box;

[0026] A reagent strip in place detection sensor is installed on the side of the frame.

[0027] In some embodiments, the magnetic separation assembly comprises:

[0028] A base, the base being mounted on the bottom of the main frame;

[0029] A magnetic separation turntable, the magnetic separation turntable is connected to the magnetic separation turntable motor in a transmission manner and rotates in a horizontal direction relative to the base under the drive of the magnetic separation turntable motor. The magnetic separation turntable is provided with an avoidance opening for the reaction tube to pass through;

[0030] A waste liquid needle, wherein there are multiple waste liquid needles, each of which is mounted on a bracket, and the bracket is connected to a needle lifting motor and drives the needle lifting motor to move in a vertical direction;

[0031] A mixing head, wherein the mixing head is drivingly connected to a mixing motor, and when the mixing head contacts the reaction tube, the mixing motor is started so that the mixing head drives the reaction tube to shake and mix;

[0032] A mixing seat, the mixing head is installed on the mixing seat, the mixing seat is transmission-connected with the mixing lifting motor, and moves in the vertical direction through the drive of the mixing lifting motor.

[0033] In some embodiments, the consumables library component includes:

[0034] A mounting seat, the mounting seat is fixed to the bottom of the main frame;

[0035] Slide rails, which are arranged on both sides of the mounting seat;

[0036] Drawers, the drawers are arranged in two groups in parallel, each group of drawers includes a reaction tube box and a TIP head box, the reaction tube box has a plurality of reaction tube installation slots, and the TIP head box has a plurality of TIP head installation slots;

[0037] An in-place detection plate is arranged on one side of the mounting seat, and the drawer abuts against the in-place detection plate when in the mounting position.

[0038] In some embodiments, the sample chamber assembly comprises:

[0039] A sample rack, the sample rack is installed on the main frame;

[0040] A barcode scanning module, the barcode scanning module is installed on the side of the sample rack;

[0041] A guide track, the guide track is arranged at the bottom of the sample rack, and a reagent rack is slidably mounted on the guide track;

[0042] An in-place detection plate is arranged at the end of the sample rack.

[0043] In some embodiments, the incubation and shaking mixing component comprises:

[0044] Incubation shaking mixing motor;

[0045] A rotating shaft, the rotating shaft is connected to the output shaft of the incubation oscillation mixing motor through an eccentric wheel transmission, and a bearing is installed on the rotating shaft;

[0046] The rotating fixed seat is installed on the installation frame, and an elliptical groove is provided on the rotating fixed seat. The bearing is arranged in the elliptical groove and moves in the elliptical groove.

[0047] In some embodiments, the light measuring component includes:

[0048] A photometric base, the photometric base is installed at the bottom of the main frame, the upper surface of the photometric base forms the photometric position, a sleeve is installed in the photometric position of the photometric base, the reaction tube is detachably installed on the sleeve, and the detection module and the detection board are both installed on the photometric base;

[0049] A light-shielding cover, the light-shielding cover is rotatably mounted on the light-measuring base in a vertical direction;

[0050] A light-shielding ring, the light-shielding ring being arranged on the outer periphery of the sleeve;

[0051] A flip cover motor is connected to the light-proof cover and drives the light-proof cover to flip in a vertical direction to cover or release the reaction tube.

[0052] In some embodiments, a zero position sensor is provided at the preset position of the X-axis guide rail and / or the preset position of the Y-axis guide rail.

[0053] In some embodiments, a gripper zero position sensor is disposed on the first Z-axis guide rail, and a syringe pump zero position sensor is disposed on the second Z-axis guide rail.

[0054] In one or more specific embodiments, the chemiluminescent immunoassay analyzer provided by the present invention has the following technical effects:

[0055] The single-portion chemiluminescent immunoassay analyzer provided by the present invention overturns the previous style of fully automatic immunoassay analyzers, that is, it retains the assembly line form to process samples, and makes an independent design for reagent packaging. The single independent cavity avoids the problem of the expiration date of the bottle opening during use, avoids reagent waste, and prevents cross contamination during the experiment. It is superior to previous single-portion immunoassay analyzers in terms of throughput, does not need to repeatedly replace consumables, is more conducive to use in small and medium-sized laboratories or inspection institutes, and improves the competitiveness of single-portion chemiluminescent immunoassay analyzers. BRIEF DESCRIPTION OF THE DRAWINGS

[0056] In order to more clearly illustrate the implementation methods of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for the implementation methods or the description of the prior art. Obviously, the drawings in the following description are only exemplary, and for ordinary technicians in this field, other implementation drawings can be derived from the provided drawings without creative work.

[0057] The structures, proportions, sizes, etc. illustrated in this specification are only used to match the contents disclosed in the specification so as to facilitate understanding and reading by persons familiar with the technology. They are not used to limit the conditions under which the present invention can be implemented, and therefore have no substantial technical significance. Any structural modification, change in proportion or adjustment of size shall still fall within the scope of the technical contents disclosed in the present invention without affecting the effects and purposes that can be achieved by the present invention.

[0058] Figure 1 This is one of the structural schematic diagrams of the chemiluminescent immunoassay analyzer provided by the present invention;

[0059] Figure 2 This is the second structural schematic diagram of the chemiluminescent immunoassay analyzer provided by the present invention;

[0060] Figure 3 The third structural schematic diagram of the chemiluminescent immunoassay analyzer provided by the present invention;

[0061] Figure 4 This is a schematic diagram of the structure of the reagent compartment assembly in the chemiluminescent immunoassay analyzer provided by the present invention;

[0062] Figure 5 This is a schematic diagram of the structure of the shuttle bus assembly in the chemiluminescent immunoassay analyzer provided by the present invention;

[0063] Figure 6 This is a schematic diagram of the structure of the magnetic separation component in the chemiluminescent immunoassay analyzer provided by the present invention;

[0064] Figure 7 This is a schematic diagram of the structure of the incubation, shaking and mixing component in the chemiluminescent immunoassay analyzer provided by the present invention;

[0065] Figure 8 This is a schematic diagram of the structure of the photometric component in the chemiluminescent immunoassay analyzer provided by the present invention;

[0066] Fig. 9 This is a schematic diagram of the structure of the consumables library component in the chemiluminescent immunoassay analyzer provided by the present invention;

[0067] Fig.10This is a schematic diagram of the structure of the sample chamber assembly in the chemiluminescent immunoassay analyzer provided by the present invention. DETAILED DESCRIPTION

[0068] The following is a description of the implementation of the present invention by specific embodiments. People familiar with the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0069] In a specific embodiment, Figure 1-Figure 3 As shown, the present invention provides a chemiluminescent immunoassay analyzer, including a three-dimensional arm component 1, a reagent compartment component 2, a magnetic separation component 3, a consumables library component 4, a sample compartment component 5, an incubation and oscillation mixing component 6, a photometric component 7, a waste discard port 8, a housing 9, a software operating system 10, a waste box 11 and a reagent temporary storage area 12.

[0070] Among them, the bottom of the main frame is provided with a reagent temporary storage area; the reagent compartment assembly stores reagent strips, and the reagent compartment assembly is installed on the main frame; the magnetic separation assembly is installed on the main frame, and the magnetic separation assembly contacts or separates from the reaction tube to drive the reaction tube to shake and mix. The magnetic separation assembly is located on the right rear side of the main frame, on the right side of the incubation and vibration mixing assembly, and on the rear side of the consumables library. During the experiment, the three-dimensional arm assembly places the reaction tube with reagents added on the incubation and vibration mixing assembly into the magnetic separation inlet. After liquid injection, mixing, waste liquid suction, and trigger liquid addition, the three-dimensional arm places the reaction tube from the magnetic separation outlet into the photometric assembly for reading.

[0071] The consumables library component is installed on the main frame and is arranged at the lower layer of the reagent compartment component. The consumables library component contains reaction tubes and TIP heads; the consumables library component is located on the front side of the whole machine, on the lower layer of the reagent compartment. When the consumables library door is pulled out, all consumable positions can be exposed for replacement and addition. The three-dimensional arm covers all consumable positions; samples are stored in the sample compartment component, and the sample compartment component is installed on the main frame; the incubation and shaking mixing component is located on the middle and rear side of the whole machine frame, on the left side of the magnetic separation component. Its main function is to serve as a temporary storage area for reaction tube sample addition. The three-dimensional arm gripper and the injection pump both cover this area.

[0072] The photometric component has a photometric position and a detection module. After the reaction tube is placed in the photometric position, the photometric data is read by the detection module. The photometric component is located at the rear side of the whole machine frame, on the left side of the incubation and shaking mixing component. The three-dimensional arm gripper covers this place to put in or take out the reaction tube; the gripper moves the added reaction tube from the magnetic separation tube outlet to the photometric position, and the photometric motor rotates to drive the light-proof cover to the reaction tube until it is pressed tightly. The reaction tube is self-luminous, and the reading component measures the value. The luminescence value is output to the host computer through the reading component for processing. After the reading is displayed, the photometric motor opens the light-proof cover in reverse, and the three-dimensional arm component gripper grabs the reaction tube and moves it to the waste bin for disposal.

[0073] The three-dimensional arm assembly includes an X-axis rail mounted on the main frame, a Y-axis rail movably mounted on the X-axis rail, and a first Z-axis rail and a second Z-axis rail movably mounted on the Y-axis rail; the gripper is movably mounted on the first Z-axis rail along the Z-axis direction, and moves within a preset range under the drive of the three-dimensional arm assembly to grab or release the reaction tube in the sample compartment to the incubation oscillation mixing assembly; the injection pump is movably mounted on the second Z-axis rail along the Z-axis direction, and moves within a preset range under the drive of the three-dimensional arm assembly. The electrical box is used to provide power supply, motor control, signal judgment, external reception of instructions, etc.

[0074] like Figure 4 As shown, the reagent bin assembly 2 includes a support frame 2.1, a shuttle assembly 2.2, a reagent kit 2.3 and a shuttle motion motor 2.4. The support frame 2.1 is mounted on the main frame. The support frame can be provided with a double-layer structure. The bottom of the bottom frame has a space for placing a waste bin and a consumables library. The space between the bottom frame and the upper frame is used to place the shuttle assembly 2.2. The reagent kit 2.3 is installed on the top of the upper frame. The reagent kit 2.3 has a plurality of reagent strip placement positions, and the reagent kit 2.3 is mounted above the support frame 2.1; the shuttle assembly 2.2 is longitudinally movably mounted on the support frame 2.1 and is located below the reagent kit 2.3; the shuttle motion motor 2.4 is connected to the shuttle assembly 2.2 in a transmission manner and drives the shuttle assembly 2.2 to move linearly to correspond to different reagent strip placement positions. It should be understood that the transmission connection mode between the shuttle assembly 2.2 and the shuttle motion motor 2.4 can be any conventional connection mode that can achieve linear motion. Furthermore, in order to ensure the accuracy of the zero position, a shuttle vehicle horizontal zero position photoelectric sensor 2.5 is also provided on the support frame. During operation, the shuttle vehicle motion motor 2.4 drives the shuttle vehicle assembly to move to the target reagent strip placement position in the reagent kit, and uses the push plate of the shuttle vehicle assembly to push the reagent strip unit at the bottom of the reagent kit, and the shuttle vehicle assembly moves to the reagent temporary storage area, waiting for the injection pump on the three-dimensional arm to absorb the reagent strip.

[0075] like Figure 5 As shown, the shuttle assembly 2.2 includes a frame 2.2.1, a push plate 2.2.2, a push plate motor 2.2.3 and a reagent strip in place detection sensor 2.2.4. The frame 2.2.1 is connected to the shuttle motion motor 2.4 by transmission. The push plate 2.2.2 is installed on the frame 2.2.1 and moves with the frame 2.2.1. The push plate motor 2.1.3 is connected to the push plate 2.2.2 by transmission and drives the push plate 2.2.2 to reciprocate relative to the frame 2.2.1 to push out the reagent strip in the test kit. The reagent strip in place detection sensor 2.2.4 is installed on the side of the frame. The shuttle assembly 2.2 is a device for transporting reagent strips. The push plate motor 2.1.3 drives the hook on the push plate 2.2.2 to move back and forth to hook the reagent strip into the interior of the shuttle. The reagent strip in place detection sensor 2.2.4 detects whether the reagent strip is in place before the next movement can be carried out. Furthermore, a push plate zero position sensor 2.2.5 may be provided on the support frame to detect the zero position of the push plate.

[0076] like Figure 6 As shown, the magnetic separation component 3 includes a base 3.2, a magnetic separation turntable 3.8, a waste liquid needle 3.9, a mixing head 3.10 and a mixing seat 3.11; wherein, the base 3.2 is installed at the bottom of the main frame, the magnetic separation turntable 3.8 is transmission-connected to the magnetic separation turntable motor 3.1, and rotates in the horizontal direction relative to the base 3.2 under the drive of the magnetic separation turntable motor 3.1, and the magnetic separation turntable 3.8 is provided with an avoidance port for the reaction tube to pass through; there are multiple waste liquid needles 3.9, each of which is installed on the bracket 3.12, and the The bracket 3.12 is transmission-connected to the needle lifting motor 3.4, and moves in the vertical direction under the drive of the needle lifting motor 3.4; the mixing head 3.10 is transmission-connected to the mixing motor 3.13, and when the mixing head 3.10 contacts the reaction tube, the mixing motor 3.13 is started so that the mixing head 3.10 drives the reaction tube to shake and mix; the mixing head 3.10 is installed on the mixing seat 3.11, and the mixing seat 3.11 is transmission-connected to the mixing lifting motor 3.6, and moves in the vertical direction through the drive of the mixing lifting motor 3.6.

[0077] In order to improve the position accuracy, the magnetic separation component also includes a magnetic separation turntable zero position sensor 3.7, a needle lift zero position sensor 3.3 and a mixing lift zero position sensor 3.5. The needle lift zero position sensor 3.3 is used to determine whether the needle lift motor 3.4 moves to the vertical zero position, the mixing lift zero position sensor 3.5 is used to determine whether the mixing component moves to the zero position, and the magnetic separation turntable zero position sensor 3.7 is used to determine whether the magnetic separation turntable moves to the zero position.

[0078] During operation, the three-dimensional arm assembly puts the added reaction tube into the tube inlet of the magnetic separation assembly 3, the magnetic separation turntable motor 3.1 rotates to drive the magnetic separation turntable 3.8 to rotate in the horizontal plane, and the external liquid adding pump adds the cleaning liquid. At this time, the mixing and lifting motor 3.6 rotates to raise the mixing motor assembly (including the mixing head 3.10 and the mixing seat 3.11), and the mixing head 3.10 supports the reaction tube, and the mixing motor 3.13 is rotated to drive the mixing head 3.10 and the reaction tube in contact with the mixing head 3.10 to do a deflection movement for mixing; after the mixing is completed, the mixing and lifting motor 3.6 rotates in the opposite direction to lower the mixing assembly from the reaction tube, and the magnetic separation turntable motor 3.1 continues to rotate to the next waste liquid needle position, and the needle lifting motor 3.4 lowers the waste liquid needle assembly (including the waste liquid needle 3.9 and the bracket 3.12), and uses the external peristaltic pump assembly to suck the waste liquid in the tube until it is sucked to the bottom, and the needle lifting motor 3.4 moves in the opposite direction to perform the operation of the next position until the magnetic separation operation is completed.

[0079] In some embodiments, Fig. 9 As shown, the consumables library assembly includes a mounting seat 4.1, a slide rail 4.2, a drawer 4.3, an in-place detection plate 4.4, a reaction tube box 4.5 and a TIP head box 4.6; wherein, the mounting seat 4.1 is fixed to the bottom of the main frame, the slide rail 4.2 is arranged on both sides of the mounting seat 4.1, the drawers 4.3 are two groups arranged in parallel, each group of drawers includes a reaction tube box 4.5 and a TIP head box 4.6, the reaction tube box 4.5 has a plurality of reaction tube mounting slots, and the TIP head box has a plurality of TIP head mounting slots; the in-place detection plate 4.4 is arranged on one side of the mounting seat 4.1, and the drawer is against the in-place detection plate 4.4 when in the installation position.

[0080] In some embodiments, Fig.10 As shown, the sample bin assembly 5 includes a sample rack 5.1, a barcode scanning module 5.2, a guide track 5.3 and an in-place detection plate 5.4. The sample rack is installed on the main frame, the barcode scanning module is installed on the side of the sample rack, the guide track is arranged at the bottom of the sample rack, a reagent rack 5.5 is slidably installed on the guide track, and the in-place detection plate is arranged at the end of the sample rack.

[0081] like Figure 7As shown, the incubation oscillation mixing component includes an incubation oscillation mixing motor 6.1, an incubation oscillation mixing motor zero position photoelectric sensor 6.2, a rotating shaft 6.3 and a rotating fixed seat 6.4, the rotating shaft is connected to the output shaft of the incubation oscillation mixing motor 6.1 through an eccentric wheel transmission, a bearing is installed on the rotating shaft, the rotating fixed seat 6.4 is installed on the mounting frame 6.5, an elliptical groove is provided on the rotating fixed seat 6.4, the bearing is arranged in the elliptical groove, and moves in the elliptical groove. During operation, the incubation oscillation mixing motor 6.1 drives the eccentric wheel to rotate, so that the rotating shaft 6.3 and the small diameter bearing move in the middle elliptical groove of the rotating fixed seat 6.4, forming an offset, and the offset (amplitude) is determined by the eccentric distance between the eccentric wheel and the rotating shaft, and the amplitude is determined by the rotation speed of the stepper motor, which can be achieved by adjusting the parameters. In order to improve the positioning accuracy, an incubation oscillation mixing motor zero position photoelectric sensor 6.2 is also provided on the mounting frame, and the zero position of the incubation oscillation mixing motor 6.1 is determined by the incubation oscillation mixing motor zero position photoelectric sensor 6.2.

[0082] like Figure 8 As shown, the photometric assembly includes a photometric base 7.1, a light-proof cover 7.5, a light-proof ring 7.6 and a flip motor 7.7. The photometric base 7.1 is installed at the bottom of the main frame, and the upper surface of the photometric base 7.1 forms the photometric position. A sleeve 7.2 is installed in the photometric position of the photometric base 7.1, and the reaction tube is detachably installed on the sleeve 7.2. The detection module 7.3 and the detection plate 7.4 are both installed on the photometric base 7.1; the light-proof cover 7.5 is rotatably installed on the photometric base 7.1 in the vertical direction, and the light-proof ring 7.6 is arranged on the outer periphery of the sleeve 7.2; the flip motor 7.7 is transmission-connected to the light-proof cover 7.5, and drives the light-proof cover 7.5 to flip in the vertical direction to cover or release the reaction tube.

[0083] Furthermore, in order to improve the measurement accuracy, the light measuring assembly also includes a flip cover zero position sensor 7.8, and the flip cover zero position sensor 7.8 is used to determine the zero position of the light-shielding cover 7.5.

[0084] During operation, the three-dimensional arm assembly drives the gripper to move the reaction tube from the magnetic separation outlet to the light measuring position, and the flip motor 7.7 rotates, driving the light-proof cover 7.5 to cover the reaction tube until it is tightened. The reaction tube emits light by itself, and the detection module 7.3 measures the value. The light-proof value is read and output to the host computer for processing through the reading assembly. After the reading is displayed, the flip motor 7.7 opens the light-proof cover 7.5 in reverse, and the three-dimensional arm assembly drives the gripper to grab the reaction tube and move it to the waste bin for disposal.

[0085] In some embodiments, the preset position of the X-axis guide rail and / or the preset position of the Y-axis guide rail are both provided with a zero position sensor, the first Z-axis guide rail is provided with a gripper zero position sensor, and the second Z-axis guide rail is provided with a syringe pump zero position sensor. The three-dimensional arm assembly 1 includes an X-axis assembly 1.1 (X-axis motion motor, X-axis slide rail and X-axis zero position sensor, etc.), a Y-axis assembly 1.2 (Y-axis motion motor, Y-axis slide rail and Y-axis zero position sensor, etc.), and a Z-axis assembly 1.3 (first Z-axis guide rail, second Z-axis guide rail, Z-axis gripper zero position sensor, Z-axis syringe pump zero position sensor, etc.).

[0086] In a specific usage scenario, when not working normally, the analyzer provided by the present invention is initialized:

[0087] Step 1: The Z-axis gripper lifting motor moves back to the vertical zero position; the Z-axis injection pump lifting motor moves back to the zero position, the Y-axis motion motor moves back to the Y-axis zero position, and the X-axis motion motor moves back to the X-axis zero position;

[0088] Step 2: The push plate motor 2.2.3 in the reagent bin module 2 moves back to the zero position; the shuttle vehicle movement motor 2.4 moves to the zero position;

[0089] Step 3: In the magnetic separation assembly 3, the needle lifting motor 3.4 moves to the vertical zero position; the magnetic separation turntable motor 3.1 moves to the zero position; and the mixing lifting motor 3.6 moves to the zero position;

[0090] Step 4: In the incubation oscillation mixing component 6, the incubation oscillation mixing motor moves to a horizontal zero position;

[0091] Step 5: The flip motor 7.7 of the light measuring module 7 drives the light-proof cover 7.5 to rotate to close the light measuring position. After the detection module 7.3 performs light measuring readings, the flip motor 7.7 moves in the reverse direction to drive the light-proof cover 7.5 back to the zero position to determine that there is no light leakage.

[0092] When the analyzer provided by the present invention works normally, the first step is: the software operating system 10 sets the reagent items, checks the status of the consumable component 4, if the quantity is insufficient, needs to add reaction tubes and TIPs, observes the overflow degree in the waste box or directly clears out all waste; the second step is: puts the set reagent strips in the reagent library component 2; the third step is: pull out the reagent rack of the reagent library component 5, puts in the sample tube, with the barcode facing left and exposed for observation, and pushes the reagent rack into the warehouse along the track of the reagent library component 5 until a bead collision sound is heard and the panel light is on; the fourth step is: click the "Start" button on the touch screen of the software operating system 10 and wait for the result.

[0093] During the experiment, the shuttle bus of the reagent library component 2 hooks the middle strip of the reagent kit, pulls it into the shuttle bus, uses the reagent compartment module motor 2.1 to quickly reciprocate left and right for a short distance, and after rapid vibration, pushes it into the reagent temporary storage area component 12 for heating. The three-dimensional arm component 1 moves to the reaction tube position, and the Z-axis gripper grabs the reaction tube to the corresponding position of the incubation vibration mixing component 6, and the gripper returns to zero; the three-dimensional arm component 1 injects the pump module to the TIP area of ​​the consumable library component 4 for loading, returns to zero and moves to the sample tube of the sample compartment component 5 to extract the sample, and incubates the reaction tube of the vibration mixing component 6. The three-dimensional arm component 1 moves to the waste box inlet to unload the TIP; reload the TIP, move to the reagent storage area of ​​the reagent strip area of ​​the reagent library component 2, absorb the magnetic beads in position 1 and the enzyme in position 2 of the reagent strip, and put them into the reaction tube of the incubation vibration mixing component 6 to start incubation Oscillate and mix until a certain time; after oscillating and mixing, the three-dimensional arm component 1 clamps the reaction tube into the magnetic separation component 3, controls the needle lifting motor 3.4 in the magnetic separation component 3 to move the steel needle up and down, and sucks and exhales the liquid for reaction; then use the gripper of the three-dimensional arm component 1 to move the reaction cup from the magnetic separation component 3 to the incubation oscillation mixing component 6, and move the injection pump of the three-dimensional arm component 1 to the temporary storage area of ​​the reagent library component 2 to absorb the substrate liquid, and add it to the reaction tube of the incubation oscillation mixing component 6. Use the injection pump and TIP combination of the Z axis of the three-dimensional arm component to suck and exhale and mix, and the gripper of the three-dimensional arm component 1 moves the reaction tube on the incubation oscillation mixing component 6 to the light measuring component 7, covers the light shield and starts to read the detection value, and opens the light shield after reading. The gripper of the three-dimensional arm component 1 discards the reaction tube after reaction into the waste box. This is a single experimental process.

[0094] The single-portion chemiluminescent immunoassay analyzer provided by the present invention overturns the previous style of fully automatic immunoassay analyzers, that is, it retains the assembly line form to process samples, and makes an independent design for reagent packaging. The single independent cavity avoids the problem of the expiration date of the bottle opening during use, avoids reagent waste, and prevents cross contamination during the experiment. It is superior to previous single-portion immunoassay analyzers in terms of throughput, does not need to repeatedly replace consumables, is more conducive to use in small and medium-sized laboratories or inspection institutes, and improves the competitiveness of single-portion chemiluminescent immunoassay analyzers.

[0095] The above specific implementation methods further illustrate the purpose, technical solutions and beneficial effects of the present invention in detail. It should be understood that the above are only specific implementation methods of the present invention and are not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc. made on the basis of the technical solutions of the present invention should be included in the scope of protection of the present invention.

Claims

1. A chemiluminescent immunoassay analyzer, characterized in that: include: A main frame, wherein the bottom of the main frame has a reagent temporary storage area; A reagent chamber assembly, wherein a reagent strip is stored in the reagent chamber assembly, and the reagent chamber assembly is mounted on the main frame; A magnetic separation component, which is mounted on the main frame and contacts or separates from the reaction tube to drive the reaction tube to shake; A consumables library component, which is installed on the main frame and arranged at the lower layer of the reagent compartment component, and contains reaction tubes and TIP heads; A sample chamber assembly, in which samples are stored, and the sample chamber assembly is installed on the main frame; An incubation and shaking mixing component, which is a temporary storage area for adding samples to the reaction tubes; A photometric component, wherein the photometric component has a photometric position and a detection module. After the reaction tube is placed in the photometric position, the photometric data is read by the detection module; A three-dimensional arm assembly, the three-dimensional arm assembly comprising an X-axis guide rail mounted on the main frame, a Y-axis guide rail movably mounted on the X-axis guide rail, and a first Z-axis guide rail and a second Z-axis guide rail movably mounted on the Y-axis guide rail; A gripper, which is movably mounted on the first Z-axis guide rail along the Z-axis direction, and moves between the waste box discarding position and the magnetic separation component under the drive of the three-dimensional arm component to grab or release the reaction tube in the sample compartment to the photometric component, the incubation and oscillation mixing component, and the magnetic separation component, and grab the reaction tube from the consumables library; The injection pump is movably mounted on the second Z-axis guide rail along the Z-axis direction, and moves from the sample area to the incubation oscillation mixing component under the drive of the three-dimensional arm assembly, and uses the TIP head to transport the sample to the reaction tube on the mixing oscillation.

2. The chemiluminescent immunoassay analyzer according to claim 1, characterized in that: The reagent compartment assembly comprises: A support frame, the support frame is installed on the main frame; A reagent kit, the reagent kit having a plurality of reagent strip placement positions, the reagent kit being installed above the support frame; A shuttle assembly, which is movably mounted on the support frame longitudinally and is located below the test kit; The shuttle vehicle motion motor is in driving connection with the shuttle vehicle assembly and drives the shuttle vehicle assembly to move linearly to correspond to different reagent strip placement positions.

3. The chemiluminescent immunoassay analyzer according to claim 2, characterized in that: The shuttle bus assembly includes: A vehicle frame, the vehicle frame being drivingly connected to the shuttle vehicle motion motor; A push plate, the push plate is mounted on the frame and moves with the frame; A push plate motor, the push plate motor is drivingly connected to the push plate and drives the push plate to reciprocate relative to the frame to push out the reagent strip in the reagent box; A reagent strip in place detection sensor is installed on the side of the frame.

4. The chemiluminescent immunoassay analyzer according to claim 1, characterized in that: The magnetic separation component comprises: A base, the base being mounted on the bottom of the main frame; A magnetic separation turntable, the magnetic separation turntable is connected to the magnetic separation turntable motor in a transmission manner and rotates in a horizontal direction relative to the base under the drive of the magnetic separation turntable motor. The magnetic separation turntable is provided with an avoidance opening for the reaction tube to pass through; A waste liquid needle, wherein there are multiple waste liquid needles, each of which is mounted on a bracket, and the bracket is connected to a needle lifting motor and drives the needle lifting motor to move in a vertical direction; A mixing head, wherein the mixing head is drivingly connected to a mixing motor, and when the mixing head contacts the reaction tube, the mixing motor is started so that the mixing head drives the reaction tube to shake and mix; A mixing seat, the mixing head is installed on the mixing seat, the mixing seat is transmission-connected with the mixing lifting motor, and moves in the vertical direction through the drive of the mixing lifting motor.

5. The chemiluminescent immunoassay analyzer according to claim 1, characterized in that: The consumables library component comprises: A mounting seat, the mounting seat is fixed to the bottom of the main frame; Slide rails, which are arranged on both sides of the mounting seat; Drawers, the drawers are arranged in two groups in parallel, each group of drawers includes a reaction tube box and a TIP head box, the reaction tube box has a plurality of reaction tube installation slots, and the TIP head box has a plurality of TIP head installation slots; An in-place detection plate is arranged on one side of the mounting seat, and the drawer abuts against the in-place detection plate when in the mounting position.

6. The chemiluminescent immunoassay analyzer according to claim 1, characterized in that: The sample chamber assembly comprises: A sample rack, the sample rack is installed on the main frame; A barcode scanning module, the barcode scanning module is installed on the side of the sample rack; A guide track, the guide track is arranged at the bottom of the sample rack, and a reagent rack is slidably mounted on the guide track; An in-place detection plate is arranged at the end of the sample rack.

7. The chemiluminescent immunoassay analyzer according to claim 1, characterized in that: The incubation oscillation mixing component comprises: Incubation shaking mixing motor; A rotating shaft, the rotating shaft is connected to the output shaft of the incubation oscillation mixing motor through an eccentric wheel transmission, and a bearing is installed on the rotating shaft; The rotating fixed seat is installed on the installation frame, and an elliptical groove is provided on the rotating fixed seat. The bearing is arranged in the elliptical groove and moves in the elliptical groove.

8. The chemiluminescent immunoassay analyzer according to claim 1, characterized in that: The light measuring component comprises: A photometric base, the photometric base is installed at the bottom of the main frame, the upper surface of the photometric base forms the photometric position, a sleeve is installed in the photometric position of the photometric base, the reaction tube is detachably installed on the sleeve, and the detection module and the detection board are both installed on the photometric base; A light-shielding cover, the light-shielding cover is rotatably mounted on the light-measuring base in a vertical direction; A light-shielding ring, the light-shielding ring being arranged on the outer periphery of the sleeve; A flip cover motor is connected to the light-proof cover and drives the light-proof cover to flip in a vertical direction to cover or release the reaction tube.

9. The chemiluminescent immunoassay analyzer according to claim 1, characterized in that: A zero position sensor is provided at the preset position of the X-axis guide rail and / or the preset position of the Y-axis guide rail.

10. The chemiluminescent immunoassay analyzer according to claim 1, characterized in that: A gripper zero position sensor is arranged on the first Z-axis guide rail, and a syringe pump zero position sensor is arranged on the second Z-axis guide rail.

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