Capillary tube array channel processing device and method

Abstract

The invention discloses a capillary array channel treatment device and method. The device comprises a constant temperature box, a control box, a one-to-six joint, a negative pressure module I, a negative pressure module II, a waste liquid tank I, a waste liquid tank II, a concentrated acid storage tank, a concentrated alkali storage tank, a dilute acid storage tank, a dilute alkali storage tank and an ultrapure water storage tank. When the device is used for treating the capillary tube array channel, the capillary tube array channel can be effectively conducted and subjected to efficient acid-base repair treatment, the automation degree is high, the treatment effect is good, and application and popularization are facilitated. The physical and chemical properties of the inner wall of the quartz capillary array channel are combined, and the capillary array channel is treated by adopting acid and alkali with different concentrations, so that the capillary array channel can be effectively repaired, the service life of the capillary array is prolonged, the consumable cost of a capillary electrophoresis technology is saved, and the method has great application value.

Description

Technical Field

[0001] The present invention relates to the technical field of capillary array maintenance, and in particular to a capillary array channel processing device and method. Background Art

[0002] The capillary array channel is an electrophoresis channel for separating fluorescently labeled DNA fragments. It is composed of multiple elastic fused silica capillaries of equal length arranged in a regular pattern. Figure 1 As shown, one end of the capillary array 100 is connected to the injection end module (101), and the other end is connected to the sample injection end module (102), and a detection module 103 with good light transmittance is provided at the end close to the injection module 101. After the capillary array is used for a period of time, the detection performance of the capillary array may be reduced or the channel may be blocked. Currently, such capillary arrays are usually directly discarded because there is no effective treatment method. Summary of the invention

[0003] In view of the deficiencies in the prior art, the present invention aims to provide a capillary array channel processing device and method.

[0004] In order to achieve the above object, the present invention adopts the following technical solution:

[0005] A capillary array channel processing device, comprising a constant temperature box, a control box, a one-to-six joint, a negative pressure module 1, a negative pressure module 2, a waste liquid tank 1, a waste liquid tank 2, a concentrated acid storage tank, a concentrated alkali storage tank, a dilute acid storage tank, a dilute alkali storage tank and an ultrapure water storage tank;

[0006] The capillary array channel to be processed is arranged in the thermostatic box, and the thermostatic box is provided with a glue injection end outlet and a sample injection end outlet, and the glue injection end module and the sample injection end module of the capillary array channel to be processed extend out of the thermostatic box through the glue injection end outlet and the sample injection end outlet respectively;

[0007] The concentrated acid storage tank, the concentrated alkali storage tank, the dilute acid storage tank, the dilute alkali storage tank and the ultrapure water storage tank are respectively provided with water pumps, and the waste liquid tank 1 and the waste liquid tank 2 are respectively connected to the negative pressure module 1 and the negative pressure module 2; the one-to-six joint includes a summary port and six branch ports, the injection end module is connected to the summary port through a transfer pipe, and the six branch ports are respectively connected to the waste liquid tank 1 and the concentrated acid storage tank, the concentrated alkali storage tank, the dilute acid storage tank, the dilute alkali storage tank and the ultrapure water storage tank through separate pipes; the injection end module is connected to the waste liquid tank 2;

[0008] The negative pressure module 1, the negative pressure module 2 and each water pump are connected to the control box, and are powered and controlled by the control box.

[0009] Furthermore, the capillary array channel processing device includes an ultrasonic cleaning device for ultrasonically cleaning the injection end module and the injection end module.

[0010] Furthermore, the control box has a negative pressure suction switch knob and a water pump switch control knob. The negative pressure suction switch knob has two control gears, which are respectively used to control the negative pressure suction to turn on and off; the water pump switch control knob has six control gears, which are respectively used to control the five water pumps to turn on and off.

[0011] The present invention also provides a capillary array channel processing method using the above device, the specific process is:

[0012] If the capillary array channel needs to be conducted, the thermostat is turned on, the temperature of the thermostat is adjusted, and the capillary array channel to be treated is heated at a constant temperature; negative pressure suction is turned on by the control box, and negative pressure module 1 and negative pressure module 2 are started to form negative pressure suction on the injection end module and the injection end module respectively, and the experimental residues blocking the capillary array channel are sucked out to conduct the capillary array channel;

[0013] If the capillary array channel needs to be repaired, the control box is used to control the water pumps in the concentrated acid storage tank, concentrated alkali storage tank, dilute acid storage tank, dilute alkali storage tank and ultrapure water storage tank to be started in sequence. A water pump is started and maintained for a set time and then turned off, and then the next water pump is started, thereby repairing the inside of the capillary array channel with concentrated acid, concentrated alkali, dilute acid, dilute alkali and ultrapure water in sequence.

[0014] Furthermore, in the above method, before starting the negative pressure suction, the injection end module and the injection end module are first ultrasonically cleaned with ultrapure water using an ultrasonic cleaning device. The water temperature is 60°C and the frequency is 30KHz. The cleaning is performed for 15 minutes to clean the experimental residues slightly adhering to the ports of the injection end module and the injection end module.

[0015] The beneficial effects of the present invention are:

[0016] 1. The device of the present invention is used to process the capillary array channel, which can achieve effective conduction and efficient acid-base repair treatment of the capillary array channel, has a high degree of automation, good treatment effect, and is conducive to promotion and application.

[0017] 2. The present invention combines the physical and chemical properties of the inner wall of the quartz capillary array channel and treats the capillary array channel with acids and alkalis of different concentrations to effectively repair the capillary array channel, so that the performance indicators of the repaired capillary array channel in spatial correction, STR detection analysis and other analyses can reach or approach the level of a new capillary array channel, thereby extending the service life of the capillary array, saving the consumables cost of the capillary electrophoresis technology, and having great application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 is a schematic diagram of the existing capillary array channel structure;

[0019] Figure 2 Schematic diagram of the structure of the capillary array channel processing device in Example 1 of the present invention;

[0020] Figure 3 This is a comparison diagram of calibration spectra for spatial calibration of the capillary array channel before and after repair in Example 2 of the present invention;

[0021] Figure 4 The STR detection analysis spectrum of the capillary array channel before repair in Example 2 of the present invention used for STR detection analysis;

[0022] Figure 5 This is the STR detection analysis spectrum of the repaired capillary array channel used for STR detection analysis in Example 2 of the present invention. DETAILED DESCRIPTION

[0023] The present invention will be further described below in conjunction with the accompanying drawings. It should be noted that this embodiment is based on the technical solution and provides a detailed implementation method and specific operation process, but the protection scope of the present invention is not limited to this embodiment.

[0024] Example 1

[0025] This embodiment provides a capillary array channel processing device, such as Figure 2 As shown, it includes a constant temperature box 1, a control box 2, a one-to-six joint 3, a negative pressure module 1 4, a negative pressure module 2 5, a waste liquid tank 1 6, a waste liquid tank 2 7, a concentrated acid storage tank 8, a concentrated alkali storage tank 9, a dilute acid storage tank 10, a dilute alkali storage tank 11 and an ultrapure water storage tank 12;

[0026] The capillary array channel 100 to be processed is arranged in the thermostat 1, and the thermostat 1 is provided with a glue injection end outlet and a sample injection end outlet, and the glue injection end module 101 and the sample injection end module 102 of the capillary array channel 100 to be processed extend out of the thermostat 1 through the glue injection end outlet and the sample injection end outlet respectively;

[0027] The concentrated acid storage tank 8, the concentrated alkali storage tank 9, the dilute acid storage tank 10, the dilute alkali storage tank 11 and the ultrapure water storage tank 12 are respectively provided with water pumps ( Figure 2(not shown), the waste liquid tank 1 6 and the waste liquid tank 2 7 are connected to the negative pressure module 1 4 and the negative pressure module 2 5 respectively; the one-to-six connector 3 includes a summary port and six branch ports, the injection end module 101 is connected to the summary port through a transfer pipe 13, and the six branch ports are connected to the waste liquid tank 1 6 and the concentrated acid storage tank 8, the concentrated alkali storage tank 9, the dilute acid storage tank 10, the dilute alkali storage tank 11 and the water pump in the ultrapure water storage tank 12 through separate pipes; the injection end module 102 is connected to the waste liquid tank 2 7;

[0028] Negative pressure module 1 4 , negative pressure module 2 5 and each water pump are connected to the control box 2 , and are powered and controlled by the control box 2 .

[0029] In this embodiment, the capillary array channel processing device may further include an ultrasonic cleaning device for ultrasonically cleaning the injection end module and the sample injection end module.

[0030] In this embodiment, the control box 2 has a negative pressure suction switch knob 21 and a water pump switch control knob 22. The negative pressure suction switch knob 21 has two control gears, which are respectively used to control the negative pressure suction to turn on and off; the water pump switch control knob 22 has six control gears, which are respectively used to control the five water pumps to turn on and off.

[0031] For example, the water pump switch control knob has six gears: 1, 2, 3, 4, 5, and 6. Gears 1, 2, 3, 4, and 5 are used to control the water pumps in the concentrated acid storage tank, concentrated alkali storage tank, dilute acid storage tank, dilute alkali storage tank, and ultrapure water storage tank, respectively. Gear 6 controls all water pumps to be turned off. When turning from gear 1 to gear 2, the water pump in the concentrated acid storage tank will be turned off, and the water pump in the concentrated alkali storage tank will be turned on, and so on.

[0032] Example 2

[0033] This embodiment provides a capillary array channel processing method using the device described in Embodiment 1, and the specific process is as follows:

[0034] Turn on the thermostat 1, adjust the temperature of the thermostat 1, and heat the capillary array channel 100 to be processed at a constant temperature;

[0035] The negative pressure suction is turned on by the control box 2, and the negative pressure module 1 4 and the negative pressure module 2 5 are started, respectively forming negative pressure suction on the injection end module 101 and the injection end module 102, sucking out the experimental residues blocking the capillary array channel 100, and conducting the capillary array channel 100;

[0036] Then, the control box 2 is used to sequentially start the water pumps in the concentrated acid storage tank 8, the concentrated alkali storage tank 9, the dilute acid storage tank 10, the dilute alkali storage tank 11 and the ultrapure water storage tank 12. One water pump is started and maintained for a set time and then turned off, and then the next water pump is started, thereby repairing the interior of the capillary array channel 100 with concentrated acid, concentrated alkali, dilute acid, dilute alkali and ultrapure water in turn.

[0037] Through the comparison of experiments with different concentrations, different sequences, different treatment durations, and different flow rates, the best treatment effects were achieved by using 1mol / L nitric acid for 30 minutes (flow rate 2.0mL / s), 1mol / L sodium hydroxide for 30 minutes (flow rate 2.0mL / s), 0.1mol / L nitric acid for 60 minutes (flow rate 0.01mL / s), 0.1mol / L sodium hydroxide for 60 minutes (flow rate 0.01mL / s), ultrapure water for 15 minutes (flow rate 2.0mL / s), and ultrapure water for 30 minutes (flow rate 0.01mL / s). It should be noted that ultrapure water can be treated for different times using different flow rates, which can be achieved by presetting the control program in the control box.

[0038] In this embodiment, the capillary array channel can be effectively repaired by treating the capillary array channel with different concentrations of acid and alkali in combination with the physical and chemical properties of the inner wall of the quartz capillary array channel, so that the performance index of the repaired capillary array channel in spatial correction, STR detection analysis and other analyses can reach or approach the level of a new capillary array channel (the calibration spectrum of the capillary array channel for spatial correction before and after repair is compared, for example). Figure 3 As shown, the STR detection analysis spectrum of the capillary array channel before repair for STR detection analysis is as follows Figure 4 As shown, the repaired capillary array channel is used for STR detection and analysis, and the STR detection and analysis spectrum is as follows Figure 5 As shown), the service life of the capillary array is extended, the consumable cost of the capillary electrophoresis technology is saved, and it has great application value.

[0039] Furthermore, before starting the negative pressure suction, the injection end module 101 and the injection end module 102 are first ultrasonically cleaned with ultrapure water using an ultrasonic cleaning device. The water temperature is 60°C and the frequency is 30KHz. The cleaning is carried out for 15 minutes to clean the experimental residues slightly adhering to the ports of the injection end module and the injection end module.

[0040] For those skilled in the art, various corresponding changes and modifications can be made according to the above technical solutions and concepts, and all of these changes and modifications should be included in the protection scope of the claims of the present invention.

Claims

1. A capillary array channel processing device, characterized in that: It comprises a constant temperature box (1), a control box (2), a one-to-six joint (3), a negative pressure module 1 (4), a negative pressure module 2 (5), a waste liquid tank 1 (6), a waste liquid tank 2 (7), a concentrated acid storage tank (8), a concentrated alkali storage tank (9), a dilute acid storage tank (10), a dilute alkali storage tank (11) and an ultrapure water storage tank (12); The capillary array channel (100) to be processed is arranged in the thermostatic box (1), and the thermostatic box (1) is provided with a glue injection end outlet and a sample injection end outlet, and the glue injection end module (101) and the sample injection end module (102) of the capillary array channel (100) to be processed extend out of the thermostatic box (1) through the glue injection end outlet and the sample injection end outlet respectively; The concentrated acid storage tank (8), the concentrated alkali storage tank (9), the dilute acid storage tank (10), the dilute alkali storage tank (11) and the ultrapure water storage tank (12) are respectively provided with water pumps; the waste liquid tank 1 (6) and the waste liquid tank 2 (7) are respectively connected to the negative pressure module 1 (4) and the negative pressure module 2 (5); the one-to-six connector (3) comprises a central port and six branch ports; the injection end module (101) is connected to the central port via a transfer pipe (13); the six branch ports are respectively connected to the waste liquid tank 1 (6) and the water pumps in the concentrated acid storage tank (8), the concentrated alkali storage tank (9), the dilute acid storage tank (10), the dilute alkali storage tank (11) and the ultrapure water storage tank (12) via separate pipes; the injection end module (102) is connected to the waste liquid tank 2 (7); Negative pressure module 1 (4), negative pressure module 2 (5) and each water pump are connected to the control box (2) and are powered and controlled by the control box (2).

2. The capillary array channel processing device according to claim 1, characterized in that: The capillary array channel processing device includes an ultrasonic cleaning device for ultrasonically cleaning the glue injection end module and the sample injection end module.

3. The capillary array channel processing device according to claim 1, characterized in that: The control box (2) has a negative pressure suction switch knob (21) and a water pump switch control knob (22); the negative pressure suction switch knob (21) has two control gears, respectively used to control the negative pressure suction to be turned on and off; the water pump switch control knob (22) has six control gears, respectively used to control the five water pumps to be turned on and off.

4. A method for processing a capillary array channel using the device according to any one of claims 1 to 3, characterized in that: The specific process is: If the capillary array channel needs to be opened, the thermostat (1) is turned on, the temperature of the thermostat (1) is adjusted, and the capillary array channel (100) to be processed is heated at a constant temperature; negative pressure suction is turned on through the control box (2), and the negative pressure module 1 (4) and the negative pressure module 2 (5) are started to form negative pressure suction on the injection end module (101) and the injection end module (102), respectively, so as to suck out the experimental residues blocking the capillary array channel (100), and open the capillary array channel (100); If the capillary array channel needs to be repaired, the control box (2) is used to sequentially control the water pumps in the concentrated acid storage tank (8), the concentrated alkali storage tank (9), the dilute acid storage tank (10), the dilute alkali storage tank (11) and the ultrapure water storage tank (12). After one water pump is started and maintained for a set time, it is turned off, and then the next water pump is turned on, thereby repairing the inside of the capillary array channel (100) with concentrated acid, concentrated alkali, dilute acid, dilute alkali and ultrapure water in sequence.

5. The method according to claim 4, characterized in that Before starting the negative pressure suction, the injection end module (101) and the injection end module (102) are first ultrasonically cleaned with ultrapure water using an ultrasonic cleaning device at a water temperature of 60°C and a frequency of 30 kHz for 15 minutes to clean any experimental residues slightly adhering to the ports of the injection end module and the injection end module.

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