A method for sowing and raising seedlings of basil grass under forest
By pretreating the seeds and soil of Baoduocao and using disease-resistant substrates prepared with rice husks and other ingredients to prevent and control pests, the pest problems of Baoduocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaocaoca
Patent Information
- Application Number
- CN202411938950.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-26
- Publication Date
- 2025-04-08
AI Technical Summary
When sowing and breeding seedlings under the forest, the under forest environment is prone to breeding diseases and pests, resulting in a decrease in germination rate and seedling quality.
Through seed pretreatment and soil pretreatment, it includes disinfecting and soaking the seeds of baoduo grass, and adding a disease-resistant matrix to the soil. The disease-resistant matrix is made of rice husks, malphuricine and other ingredients. It is prepared into a granular type through xanthan gum as a binder and mixed into the soil to prevent and control pests and diseases.
It effectively prevents and controls the pests and diseases of Baoduo grass when growing seedlings under the forest, improves the germination rate of seeds and the quality of seedlings, and extends the insecticidal and bactericidal effect time of the disease-resistant matrix.
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Figure CN119769236A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of seedling cultivation of hyssop, and in particular to a method for sowing and raising seedlings of hyssop under forests. Background Art
[0002] Baoduo grass is also known as light bamboo flower. It belongs to the perennial herbaceous plant of the genus Wanshou bamboo of the Liliaceae family. It is distributed in Shaanxi, Yunnan, North China, South China and other places in my country. The rhizome and root of Baoduo grass can be used as medicine. It tastes sweet and light, and has a neutral nature. It has the effects of moistening the lungs and relieving coughs, strengthening the spleen and digestion, relaxing muscles and activating collaterals, clearing away heat and detoxifying. It has good medicinal value and can also be planted in the ground or potted for viewing. Due to the extensive collection of Baoduo grass by people for many years, coupled with environmental pollution and damage, the distribution range and number of this plant are getting smaller and smaller.
[0003] The main propagation method of Baoduo grass is seed propagation, and Baoduo grass grows under the forest or in the bushes. It likes a cool and moist environment and grows best in loose, fertile sandy soil. Therefore, sowing and raising Baoduo grass seedlings under the forest can not only better meet the growth environment requirements of Baoduo grass, but also make full use of the spatial resources of the forest land, reduce land occupation, and cultivate a large number of Baoduo grass seedlings to promote the development of the Baoduo grass industry. However, when planting Baoduo grass under the forest, the cool and moist environment of the shade under the forest is prone to breed pests and diseases, which harm Baoduo grass seeds and seedlings, thereby reducing the germination rate and seedling quality.
[0004] Therefore, there is a need to find a method for sowing and raising seedlings of basil under the forest to solve the problem that the forest environment is prone to breeding diseases and pests when basil is sown and raised seedlings under the forest, thereby reducing the germination rate and seedling quality. Summary of the invention
[0005] In view of this, the purpose of the present invention is to provide a method for sowing and raising seedlings of Bauhinia ovata under the forest, so as to solve the problem that the understory environment is prone to breed diseases and pests during sowing and raising seedlings of Bauhinia ovata under the forest, thereby reducing the germination rate and seedling quality.
[0006] The present invention solves the above technical problems by the following technical means:
[0007] A method for sowing and raising seedlings of basil grass under forests, the method being as follows:
[0008] (1) Seed pretreatment: sterilize the seeds of the herbaceous plant, and then soak the sterilized seeds in water for 24 hours to obtain pretreated seeds;
[0009] (2) Pretreatment of the soil under the forest: After removing weeds and impurities from the soil under the forest, the soil is plowed, and then the disease-resistant matrix is sprinkled on the surface of the plowed soil and rotary tilled at a depth of 5 to 8 cm to obtain the pretreated soil;
[0010] (3) Sowing: Pre-treated seeds were sown at 2 g / m2 The amount of seeds can be sown in the pretreated soil, and then maintained according to the conventional methods.
[0011] Further, the disinfection method in step (1) is as follows:
[0012] Select seeds of the herb with full grains, soak them in a 75% by volume ethanol solution for 30 to 60 seconds, transfer them into a 0.1 wt% mercuric chloride solution for disinfection for 3 to 5 minutes, and wash them with water for 5 times to obtain the disinfected seeds.
[0013] Furthermore, the anti-disease matrix includes the following raw materials:
[0014] Rice husk, tert-butylethanolamine, tetraethyl orthosilicate, ammonia water, ethanol, xanthan gum, 3-dimethylaminoacrolein, matrine, wood vinegar, amino oligosaccharides.
[0015] Further, the preparation method of the disease-resistant matrix is as follows
[0016] A: The rice husk is immersed in water, tert-butylethanolamine is added, the pH is adjusted to 9-10, and then the rice husk is boiled for 1-4 hours, cooled to room temperature and then allowed to stand overnight to obtain expanded rice husk, and then the expanded rice husk is drained and immersed in a 30% ethanol solution by volume, ammonia water and tetraethyl orthosilicate are added, the temperature is raised to 50-60° C. and stirred for reaction for 30 minutes, after the reaction is completed, the rice husk is cooled to room temperature and allowed to stand for 4-6 hours, and then dried to obtain the pretreated rice husk;
[0017] B: adjusting the moisture content of the pretreated rice husk to 60-70% and the pH to 5.5-6.5, and then fermenting for 15-20 days, then adding matrine, wood vinegar and 10 wt% amino oligosaccharide solution and stirring evenly to obtain a mixed material;
[0018] C: Dissolve xanthan gum in water with a mass of 10 times the mass, then add 3-dimethylaminoacrolein, stir and react for 2 to 4 hours, and after the reaction is completed, let it stand overnight to obtain the pretreated xanthan gum solution; heat the pretreated xanthan gum solution to 70°C and maintain constant temperature for 30 minutes, then add it to the mixed material and stir evenly, let it stand for 1 day, put it into a granulator for granulation, and then dry it to obtain the disease-resistant matrix.
[0019] The present invention uses rice husk as a base material, mixes matrine and other substances to prepare a disease-resistant matrix, mixes it into the soil, and prevents and controls pests and pathogens in the soil under the forest, so as to ensure that the baoduo grass is protected from pests and diseases in the cool and moist environment under the forest, thereby improving the yield and quality of seedlings. Since it takes a long time from sowing to germination, in order to extend the insecticidal and bactericidal effect time, the present invention processes the rice husk, and then uses xanthan gum as a binder to wrap matrine and other substances to prepare a granular disease-resistant matrix, so as to extend the insecticidal and disease-resistant effect time of the matrix.
[0020] Specifically, the invention uses rice husks that decompose slowly as a base material, and mixes them with matrine, wood vinegar, amino oligosaccharides and other ingredients to form a mixed material, and then uses xanthan gum with strong anti-biodegradability as a binder to mix and granulate to obtain a disease-resistant matrix. The base material rice husks and the binder xanthan gum have good stability in the soil and can slowly release the effective ingredients matrine, wood vinegar and amino oligosaccharides for a long time, ensuring the insecticidal and sterilization time to ensure that seeds and seedlings are protected from diseases and pests, and at the same time can improve the physical and chemical properties of the soil under the forest.
[0021] However, rice husk is a relatively large block structure. After being prepared into a granular disease-resistant matrix, its block structure will produce a large mass transfer barrier effect on substances such as matrine and wood vinegar embedded inside, inhibiting the transmission and release of effective ingredients to the outside. Therefore, the present invention processes the rice husk to reduce its mass transfer barrier effect. Specifically, the rice husk is immersed in water, tert-butylethanolamine is added and the pH value is adjusted before cooking, and then the rice husk is transferred to an ethanol solution, ammonia water and tetraethyl orthosilicate are added for heating reaction, and then cooled and allowed to stand. In this process, after the rice husk is cooked and expanded, the tert-butylethanolamine competitively binds to the surface of the rice husk, destroys the intermolecular connection of the components on the surface of the rice husk, increases the intermolecular distance, and thus maintains its expandability to obtain expanded and loose rice husk. Furthermore, the porous product formed by the hydrolysis and polycondensation of the added tetraethyl orthosilicate is bound to the expanded and loose rice husk and embedded in the expanded and loose rice husk, thereby obtaining a loose and expanded pretreated rice husk containing a porous structure, and the internal effective ingredients, matrine, wood vinegar, and amino oligosaccharides, can be transported to the outside through the loose pores, thereby reducing the mass transfer barrier effect of large pieces of rice husk on the effective ingredients.
[0022] The present invention also uses 3-dimethylaminoacrolein to treat the binder xanthan gum. 3-dimethylaminoacrolein reduces the light oxidation stability of the xanthan gum by changing the molecular chain structure of the xanthan gum. Under weak light conditions under the forest, light attacks the binder xanthan gum and gradually breaks its molecular chain, thereby reducing its viscosity. Therefore, when the effective cost content of matrine and the like is reduced in the later stage, the later sustained release amount can be guaranteed, and the disease and insect prevention effect can be guaranteed.
[0023] Furthermore, in step A, the mass ratio of rice husk to tert-butylethanolamine is (3-6): (0.06-0.1).
[0024] Furthermore, in step A, the mass ratio of expanded rice husk, ammonia water and tetraethyl orthosilicate is (3-6): (0.15-0.3): (0.1-0.2).
[0025] Furthermore, in step B, the mass ratio of the pretreated rice husk, matrine, wood vinegar, and 10 wt% amino oligosaccharide solution is (3-6): (0.2-0.4): (1-2): (1-2).
[0026] Furthermore, in step C, the mass ratio of xanthan gum to 3-dimethylaminoacrolein is (0.05-0.1): (0.001-0.002).
[0027] Furthermore, in step C, the mass ratio of the pretreated xanthan gum solution to the mixed material is (0.5-1):(3-6).
[0028] Furthermore, the amount of the anti-disease matrix applied is 0.5 to 1 kg / m 2 .
[0029] Beneficial effects:
[0030] 1. The present invention sows and raises seedlings of baculocarpus in a cool and humid forest, which can better meet the environmental conditions required for the growth of baculocarpus, thereby promoting the germination and growth of baculocarpus, and efficiently cultivating seedlings of good quality; in addition, raising seedlings under the forest can also save land and reduce production costs.
[0031] 2. The present invention also prepares a disease-resistant matrix for application during the cultivation of Baoduo grass seedlings. The disease-resistant matrix can effectively prevent and control diseases and pests for a long time, thereby ensuring that Baoduo grass is protected from diseases and pests in the cool and humid environment under the forest, better ensuring the growth conditions of Baoduo grass, promoting the germination of Baoduo grass and improving the quality of seedlings. BRIEF DESCRIPTION OF THE DRAWINGS
[0032] Figure 1 : Fruit pictures collected by the present invention for collecting seeds for sowing;
[0033] Figure 2 : This is a picture of the seedlings of the grass cultivated in Example 4 of the present invention;
[0034] Figure 3 : This is a picture of the growth status of the seedlings of the Herba Lycopodii cultivated in Example 4 of the present invention after being transplanted into a substrate pot. DETAILED DESCRIPTION
[0035] The present invention will be described in detail below with reference to specific embodiments and accompanying drawings:
[0036] Example 1: Preparation of anti-disease matrix
[0037] A: 4 kg of rice husks were immersed in water so that the water just covered the rice husks, 0.08 kg of tert-butylethanolamine was added, the pH was adjusted to 9.5 and then boiled for 2 hours, cooled to room temperature and allowed to stand overnight to obtain expanded rice husks, which were removed and drained, and then 4 kg of expanded rice husks were immersed in a 30% ethanol solution by volume so that the solution just covered the rice husks, 0.2 kg of ammonia water and 0.15 kg of tetraethyl orthosilicate were added, the temperature was raised to 55°C and stirred for reaction for 30 minutes, after the reaction was completed, the mixture was cooled to room temperature and allowed to stand for 5 hours, and then dried at 45°C to obtain pretreated rice husks;
[0038] B: Take 4 kg of pretreated rice husk, adjust the water content to 65%, pH to 6, cover with film and ferment for 20 days, then add 0.3 kg of matrine, 1.5 kg of wood vinegar and 1.5 kg of 10 wt% amino oligosaccharide solution and stir evenly to obtain a mixed material;
[0039] C: Add 0.07kg of xanthan gum into 0.7kg of water to dissolve, then add 0.0015kg of 3-dimethylaminoacrolein, stir and react for 3h, after the reaction is completed, let it stand overnight to obtain the pretreated xanthan gum solution; take 0.7kg of the pretreated xanthan gum solution, heat it to 70℃ and keep the constant temperature for 30min, then add 4kg of the mixed material and stir to mix evenly, after standing for 1 day, put it into a granulator to prepare particles with a particle size of about 0.4cm, and dry it at 45℃ until the moisture content is about 15% to obtain the disease-resistant matrix.
[0040] Example 2: Preparation of disease-resistant matrix II
[0041] A: 3 kg of rice husks were immersed in water so that the water just covered the rice husks, 0.06 kg of tert-butylethanolamine was added, the pH was adjusted to 9 and then boiled for 2 h, cooled to room temperature and then allowed to stand overnight to obtain expanded rice husks, which were removed and drained, and then 3 kg of expanded rice husks were immersed in a 30% ethanol solution by volume so that the solution just covered the rice husks, 0.15 kg of ammonia water and 0.1 kg of tetraethyl orthosilicate were added, the temperature was raised to 50 ° C and stirred for reaction for 30 min, after the reaction was completed, the mixture was cooled to room temperature and allowed to stand for 4 h, and then dried at 45 ° C to obtain pretreated rice husks;
[0042] B: Take 3 kg of pretreated rice husk, adjust the water content to 60%, pH to 5.5, cover with film and ferment for 15 days, then add 0.2 kg of matrine, 1 kg of wood vinegar and 1 kg of 10 wt% amino oligosaccharide solution and stir evenly to obtain a mixed material;
[0043] C: Add 0.05kg of xanthan gum into 0.5kg of water to dissolve, then add 0.001kg of 3-dimethylaminoacrolein, stir and react for 2h, let stand overnight after the reaction is completed to obtain pretreated xanthan gum solution; take 0.5kg of pretreated xanthan gum solution, heat it to 70℃ and keep constant temperature for 30min, then add 3kg of mixed material and stir to mix evenly, let stand for 1 day, put it into a granulator to prepare particles with a particle size of about 0.4cm, and dry it at 45℃ until the moisture content is about 15% to obtain disease-resistant matrix.
[0044] Example 3: Preparation of disease-resistant matrix III
[0045] A: 6 kg of rice husks were immersed in water so that the water just covered the rice husks, 0.1 kg of tert-butylethanolamine was added, the pH was adjusted to 10 and then boiled for 4 hours, cooled to room temperature and then allowed to stand overnight to obtain expanded rice husks, which were removed and drained, and then 6 kg of expanded rice husks were immersed in a 30% ethanol solution by volume so that the solution just covered the rice husks, 0.3 kg of ammonia water and 0.2 kg of tetraethyl orthosilicate were added, the temperature was raised to 60°C and stirred for reaction for 30 minutes, after the reaction was completed, the mixture was cooled to room temperature and allowed to stand for 6 hours, and then dried at 45°C to obtain pretreated rice husks;
[0046] B: 6 kg of pretreated rice husks were taken, the water content was adjusted to 70%, the pH to 6.5, and then covered with a film for fermentation for 20 days, and then 0.4 kg of matrine, 2 kg of wood vinegar and 2 kg of 10 wt% amino oligosaccharide solution were added and stirred to obtain a mixed material;
[0047] C: Add 0.1kg of xanthan gum to 1kg of water to dissolve, then add 0.002kg of 3-dimethylaminoacrolein, stir and react for 4h, after the reaction is completed, let it stand overnight to obtain the pretreated xanthan gum solution; take 1kg of the pretreated xanthan gum solution, heat it to 70℃ and keep the constant temperature for 30min, then add 6kg of the mixed material and stir to mix evenly, after standing for 1 day, put it into a granulator to prepare particles with a particle size of about 0.4cm, and dry it at 45℃ until the moisture content is about 15% to obtain the disease-resistant matrix.
[0048] Comparative Example 1: Preparation of disease-resistant matrix
[0049] In contrast to Example 1, the only difference is that in the preparation of the disease-resistant matrix in Comparative Example 1, cooking is not performed in step A, as shown below:
[0050] A: 4 kg of rice husks were immersed in water so that the water just covered the rice husks, 0.08 kg of tert-butylethanolamine was added, the pH was adjusted to 9.5 and then allowed to stand overnight to obtain expanded rice husks, which were removed and drained, and then 4 kg of expanded rice husks were immersed in a 30% ethanol solution by volume so that the solution just covered the rice husks, 0.2 kg of ammonia water and 0.15 kg of tetraethyl orthosilicate were added, the temperature was raised to 55°C and stirred for reaction for 30 min, after the reaction was completed, the mixture was cooled to room temperature and allowed to stand for 5 h, and then dried at 45°C to obtain pretreated rice husks;
[0051] B~C: Same as Example 1.
[0052] Comparative Example 2: Preparation of disease-resistant matrix
[0053] In contrast to Example 1, the only difference is that in the preparation of the disease-resistant matrix in Comparative Example 2, only the rice husk is boiled in step A without adding tert-butylethanolamine, as shown below:
[0054] A: 4 kg of rice husks were immersed in water so that the water just covered the rice husks, the pH was adjusted to 9.5 and then boiled for 2 hours, cooled to room temperature and allowed to stand overnight to obtain expanded rice husks, which were removed and drained, and then 4 kg of expanded rice husks were immersed in a 30% ethanol solution by volume so that the solution just covered the rice husks, 0.2 kg of ammonia water and 0.15 kg of ethyl orthosilicate were added, the temperature was raised to 55°C and stirred for reaction for 30 minutes, after the reaction was completed, the mixture was cooled to room temperature and allowed to stand for 5 hours, and then dried at 45°C to obtain pretreated rice husks;
[0055] B~C: Same as Example 1.
[0056] Comparative Example 3: Preparation of disease-resistant matrix
[0057] In contrast to Example 1, the only difference is that in Comparative Example 3, the disease-resistant matrix preparation lacks step A, and the rice husk is not pretreated, but conventional rice husk is used, as shown below:
[0058] A: Take 4 kg of rice husk, adjust the water content to 65%, pH to 6, cover with film and ferment for 20 days, then add 0.3 kg of matrine, 1.5 kg of wood vinegar and 1.5 kg of 10 wt% amino oligosaccharide solution and stir evenly to obtain a mixed material;
[0059] B: Add 0.07kg xanthan gum into 0.7kg water to dissolve, then add 0.0015kg 3-dimethylaminoacrolein, stir and react for 3h, after the reaction is completed, let it stand overnight to obtain the pretreated xanthan gum solution; take 0.7kg pretreated xanthan gum solution, heat it to 70℃ and keep constant temperature for 30min, then add 4kg mixed material and stir to mix evenly, after standing for 1 day, put it into a granulator to prepare particles with a particle size of about 0.4cm, and dry it at 45℃ until the moisture content is about 15% to obtain the disease-resistant matrix.
[0060] Comparative Example 4: Preparation of disease-resistant matrix
[0061] In contrast to Example 1, the only difference is that in the preparation of the disease-resistant matrix in Comparative Example 4, fermentation is not performed in step B, but matrine, wood vinegar and 10 wt% amino oligosaccharide solution are directly added, as shown below:
[0062] A: Same as Example 1;
[0063] B: Take 4 kg of pretreated rice husk, adjust the water content to 65%, pH to 6, add 0.3 kg of matrine, 1.5 kg of wood vinegar and 1.5 kg of 10 wt% amino oligosaccharide solution, stir evenly to obtain a mixed material;
[0064] C: Same as Example 1.
[0065] Comparative Example 5: Preparation of disease-resistant matrix
[0066] In contrast to Example 1, the only difference is that in Comparative Example 5, 3-dimethylaminoacrolein is not added in step C when preparing the anti-disease matrix, as shown below:
[0067] A~B: Same as Example 1
[0068] C: Add 0.07 kg of xanthan gum into 0.7 kg of water to dissolve it to obtain xanthan gum solution; take 0.7 kg of xanthan gum solution, heat it to 70°C and keep it at a constant temperature for 30 minutes, then add 4 kg of the mixed material and stir to mix evenly, after standing for 1 day, put it into a granulator to prepare particles with a particle size of about 0.4 cm, and dry it at 45°C until the moisture content is about 15% to obtain a disease-resistant matrix.
[0069] Comparative Example 6: Preparation of disease-resistant matrix
[0070] In contrast to Example 1, the only difference is that in Comparative Example 6, xanthan gum is not used in the preparation of the disease-resistant matrix, but gum arabic is used, and 3-dimethylaminoacrolein is not added, as shown below:
[0071] A~B: Same as Example 1;
[0072] C: Add 0.07 kg of gum arabic into 0.7 kg of water to dissolve to obtain gum arabic liquid; take 0.7 kg of gum arabic liquid, heat it to 70°C and keep it at a constant temperature for 30 minutes, then add 4 kg of the mixed material and stir to mix evenly, after standing for 1 day, put it into a granulator to prepare particles with a particle size of about 0.4 cm, and dry it at 45°C until the moisture content is about 15% to obtain the disease-resistant matrix.
[0073] Comparative Example 7: Preparation of disease-resistant matrix
[0074] In contrast to Example 1, the only difference is that in Comparative Example 7, no xanthan gum solution is added during the preparation of the disease-resistant matrix, and the mixed material is directly granulated to prepare the disease-resistant matrix, as shown below:
[0075] A~B: Same as Example 1;
[0076] C: The mixed material is placed in a granulator to prepare granules with a particle size of about 0.4 cm, and dried at 45° C. until the moisture content is about 15% to obtain a disease-resistant matrix.
[0077] Example 4: Method for sowing and raising seedlings of Psoralea corylifolia under forest
[0078] (1) Seed pretreatment: Select seeds of Psoralea corylifolia with full grains, soak them in a 75% by volume ethanol solution for 40 seconds, then transfer them to a 0.1 wt% mercuric chloride solution for disinfection for 5 minutes, and wash them with water for 5 times to obtain disinfected seeds; soak the disinfected seeds in water for 24 hours to obtain pretreated seeds;
[0079] (2) Pretreatment of forest soil: After removing weeds and impurities from the forest soil, the soil is plowed to a depth of about 20 cm, and then the surface of the plowed soil is heated at 0.6 kg / m 2 The amount of the disease-resistant matrix prepared by the method of Example 1 is sprinkled, and then rotary tillage is performed at a depth of about 6 cm to obtain pretreated soil;
[0080] (3) Sowing: Pre-treated seeds were sown at 2 g / m 2 The amount of seeds can be sown in the pretreated soil, and then maintained according to the conventional methods.
[0081] Experiment: Experiment on sowing and raising seedlings of basil grass under forest
[0082] 1. The understory sowing and seedling raising experiment of Bodo grass was carried out in the experimental field of Huachao Park, Xi'an Botanical Garden, Shaanxi Province. The experiment was divided into 9 groups: experimental group 1, control groups 1 to 7, and blank control group. The seedling raising methods of each group were as follows:
[0083] Experimental group 1: using the disease-resistant matrix prepared in Example 1 and the seedling raising method in Example 4;
[0084] Control groups 1 to 7: using the disease-resistant substrates of Comparative Examples 1 to 7 and the seedling raising method of Example 4 respectively;
[0085] Blank control group: no anti-disease matrix was used, and the remaining steps were carried out according to the method of Example 4.
[0086] 2. 60 seeds of Psoralea corylifolia were sown in each group. 5 days after sowing, 20 aphids and 10 white grubs of the same growth were inoculated in the soil of each group. The matrine content in the soil was tested on the 5th, 13th and 30th days after sowing. The seed germination rate of each group was calculated 25 days after sowing. The incidence of seedling diseases and insect pests in each group was calculated 2 months after sowing. The data are shown in Table 1.
[0087] Table 1
[0088]
[0089]
[0090] According to the data analysis in Table 1:
[0091] (1) In the experimental group 1, 30 days after sowing, the content of matrine detected in the soil was 0.82 g / kg, indicating that the disease-resistant matrix prepared by the present invention can slowly release the insecticidal and fungicidal components over a long period of time, thereby better preventing and controlling diseases and insect pests. Furthermore, the germination rate of the seeds of the hyacinthus annuus in the experimental group 1 reached 93.3%, and the incidence rate of diseases and insect pests in the seedlings was only 7.1%, indicating that the disease-resistant matrix prepared according to the method of the present invention and mixed into the soil can make the hyacinthus annuus seeds germinate better under the forest, reduce the invasion of diseases and insect pests, and improve the germination rate and seedling quality of the hyacinthus annuus.
[0092] (2) In the control group 1, the rice husk was not boiled in step A when the disease-resistant matrix was prepared. In the control group 2, tert-butylethanolamine was not added to treat the rice husk. In the control group 3, the rice husk was not pretreated when the disease-resistant matrix was prepared. The rice husk in the control groups 1, 2 and 3 had low swelling degrees, which affected the release of the internal effective ingredients, resulting in the effective ingredients failing to enter the soil better in the early stage to exert their effects. The seeds and seedlings were affected by diseases and pests, the germination rate was reduced, and the incidence of seedling diseases and pests increased. In the control group 4, the rice husk was not fermented when the disease-resistant matrix was prepared. Instead, the unfermented rice husk entered the soil and fermented, causing diseases and pests and poisoning the seeds, resulting in a reduced germination rate and an increased incidence.
[0093] (3) In the control group 5, 3-dimethylaminoacrolein was not added to the xanthan gum during the preparation of the disease-resistant matrix. The xanthan gum was highly stable, and the structure of the disease-resistant matrix remained relatively intact in the soil, affecting the release of effective ingredients in the later stage. In the control group 6, xanthan gum was replaced with gum arabic during the preparation of the disease-resistant matrix. The xanthan gum was poorly stable in the soil, and the particle structure of the disease-resistant matrix quickly disintegrated and dispersed. In the control group 7, xanthan gum was not added during the preparation of the disease-resistant matrix. The single rice husk had poor granulation properties and quickly disintegrated and dispersed in the soil. The particle structure stability of the disease-resistant matrix in the control groups 6 and 7 was poor, resulting in the rapid release and decomposition of the effective bactericidal and insecticidal components in the early and middle stages, which shortened the action time and caused an increase in the incidence rate.
[0094] (4) In the blank control group, the germination rate of the seeds of the grass was low, and the morbidity rate was greatly increased, indicating that when the grass was sown and seedled under the forest, it was greatly affected by diseases and insect pests. Adding the disease-resistant matrix prepared by the method of the present invention can effectively reduce the impact of diseases and insect pests and improve the germination rate and seedling quality.
[0095] The above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the purpose and scope of the technical solutions of the present invention, which should be included in the scope of the claims of the present invention. The techniques, shapes, and structural parts not described in detail in the present invention are all known technologies.
Claims
1. A method for sowing and raising seedlings of basil grass under forest, characterized in that: The method is as follows: (1) Seed pretreatment: sterilize the seeds of the herbaceous plant, and then soak the sterilized seeds in water for 24 hours to obtain pretreated seeds; (2) Pretreatment of the soil under the forest: After removing weeds and impurities from the soil under the forest, the soil is plowed, and then the disease-resistant matrix is sprinkled on the surface of the plowed soil and rotary tilled at a depth of 5 to 8 cm to obtain the pretreated soil; (3) Sowing: Pre-treated seeds were sown at 2 g / m 2 The amount of seeds can be sown in the pretreated soil, and then maintained according to the conventional methods.
2. The method for sowing and raising seedlings of Psoralea corylifolia under forest according to claim 1, characterized in that: The disinfection method in step (1) is as follows: Select seeds of the herb with full grains, soak them in a 75% by volume ethanol solution for 30 to 60 seconds, transfer them into a 0.1 wt% mercuric chloride solution for disinfection for 3 to 5 minutes, and wash them with water for 5 times to obtain the disinfected seeds.
3. The method for sowing and raising seedlings of Psoralea corylifolia under forest according to claim 2, characterized in that: The anti-disease matrix comprises the following raw materials: Rice husk, tert-butylethanolamine, tetraethyl orthosilicate, ammonia water, ethanol, xanthan gum, 3-dimethylaminoacrolein, matrine, wood vinegar, amino oligosaccharides.
4. The method for sowing and raising seedlings of Psoralea corylifolia under forest according to claim 3, characterized in that: The method for preparing the disease-resistant matrix is as follows A: The rice husk is immersed in water, tert-butylethanolamine is added, the pH is adjusted to 9-10, and then the rice husk is boiled for 1-4 hours, cooled to room temperature and then allowed to stand overnight to obtain expanded rice husk, and then the expanded rice husk is drained and immersed in a 30% ethanol solution by volume, ammonia water and tetraethyl orthosilicate are added, the temperature is raised to 50-60° C. and stirred for reaction for 30 minutes, after the reaction is completed, the rice husk is cooled to room temperature and allowed to stand for 4-6 hours, and then dried to obtain the pretreated rice husk; B: adjusting the moisture content of the pretreated rice husk to 60-70% and the pH to 5.5-6.5, and then fermenting for 15-20 days, then adding matrine, wood vinegar and 10 wt% amino oligosaccharide solution and stirring evenly to obtain a mixed material; C: Dissolve xanthan gum in water with a mass of 10 times the mass, then add 3-dimethylaminoacrolein, stir and react for 2 to 4 hours, and after the reaction is completed, let it stand overnight to obtain the pretreated xanthan gum solution; heat the pretreated xanthan gum solution to 70°C and maintain constant temperature for 30 minutes, then add it to the mixed material and stir evenly, let it stand for 1 day, put it into a granulator for granulation, and then dry it to obtain the disease-resistant matrix.
5. The method for sowing and raising seedlings of Psoralea corylifolia under forest according to claim 4, characterized in that: In the step A, the mass ratio of rice husk to tert-butylethanolamine is (3-6): (0.06-0.1).
6. The method for sowing and raising seedlings of Psoralea corylifolia under forests according to claim 5, characterized in that: In the step A, the mass ratio of expanded rice husk, ammonia water and tetraethyl orthosilicate is (3-6): (0.15-0.3): (0.1-0.2).
7. The method for sowing and raising seedlings of Psoralea corylifolia under forests according to claim 6, characterized in that: The mass ratio of the pretreated rice husk, matrine, wood vinegar, and 10 wt% amino oligosaccharide solution in step B is (3-6): (0.2-0.4): (1-2): (1-2).
8. The method for sowing and raising seedlings of Psoralea corylifolia under forests according to claim 7, characterized in that: The mass ratio of xanthan gum to 3-dimethylaminoacrolein in step C is (0.05-0.1): (0.001-0.002).
9. The method for sowing and raising seedlings of Psoralea corylifolia under forests according to claim 8, characterized in that: The mass ratio of the pretreated xanthan gum solution to the mixed material in step C is (0.5-1):(3-6).
10. The method for sowing and raising seedlings of Psoralea corylifolia under forests according to claim 9, characterized in that: The amount of anti-disease matrix applied is 0.5-1 kg / m 2 .
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