Cutting propagation method for ormosia rubescens

Abstract

The invention discloses a cutting propagation method for ormosia rubescens, and belongs to the technical field of cutting of ormosia rubescens, the method comprises the steps of cutting slip collection, cutting slip pretreatment, cutting and the like, water and fertilizer regulation particles are prepared and mixed into a matrix, and the water and fertilizer regulation particles can promote absorption of nutrients and moisture in the matrix by the cutting slips in a long-acting mode and promote rooting, so that the survival rate of the cutting slips is increased; the cutting rooting time is shortened, meanwhile, absorption and utilization of nutrients and water of the cutting slips are guaranteed, the problem that the cutting slips die due to water and nutrient deficiency caused by long rooting time of the red beans is solved, the cutting survival rate of the red beans can be effectively increased, and the method has important significance on resource expansion of the red beans.

Description

Technical Field

[0001] The invention relates to the technical field of rock-growing red bean cuttings, and in particular to a rock-growing red bean cutting propagation method. Background Art

[0002] Ormosia saxatilis KMLan is an evergreen tree of the Leguminosae family. With its beautiful shape and evergreen crown, it is an ornamental tree species for garden greening. With its straight trunk, excellent material and beautiful texture, it is also a timber tree species. Its seeds have the effects of clearing away heat and detoxifying, reducing swelling and removing dampness, and are an important medicinal material.

[0003] There are few large rock-growing red bean trees with a low fruit-setting rate, and the seeds have aril, which is airtight and watertight, with weak germination ability, and seed propagation is not ideal. Cutting propagation is one of the ways of asexual reproduction of plants. It has the advantages of low cost, no restrictions on plant species, simple and easy operation, easy industrialization and fast propagation. It is an ideal propagation method for rapid propagation of rock-growing red bean. However, the growth of rock-growing red bean cuttings is extremely slow in the early stage. Under conventional methods, it takes 3 months to grow new roots. Therefore, under conventional cutting propagation methods, rock-growing red bean has no root system to absorb water and nutrients for a long time, resulting in water and nutrient deficiency causing the death of cuttings, reducing the efficiency of cutting propagation.

[0004] Therefore, it is currently necessary to find a rock bean cutting propagation method to solve the problem that the rock bean cuttings grow slowly in the initial stage under the conventional method, and the cuttings are easily killed due to water and nutrient deficiency in the absence of a root system, thereby reducing the survival rate of the cuttings. Summary of the invention

[0005] In view of this, the purpose of the present invention is to provide a rock bean cutting propagation method to solve the problem that the rock bean cuttings grow slowly in the initial stage under the conventional method, and the cuttings are easily killed due to water and nutrient deficiency in the absence of a root system, thereby reducing the survival rate of the cuttings.

[0006] The present invention solves the above technical problems by the following technical means:

[0007] A method for propagating rock bean by cuttings, the method comprising the following steps:

[0008] (1) Cutting collection: From April to June, collect healthy, disease-free, and insect-free one-year-old lignified rock bean branches from healthy mother trees. After the branches are collected, cut them into 10-20 cm segments, cut flat at the top and obliquely at the bottom, and retain 3-4 buds to obtain cuttings;

[0009] (2) Pretreatment of cuttings: The cuttings were immersed in a 0.1 wt% potassium permanganate solution for disinfection for 10 to 15 minutes, and then washed with water for 2 to 3 times after disinfection, and then the lower end of the cuttings was immersed in a 300 mg / L NAA solution for 1 to 2 hours to obtain pretreated cuttings;

[0010] (3) Cutting: Mix the substrate and water-fertilizer regulating particles evenly and put them into the seedling pot. Then insert the pretreated cuttings into the substrate. The cutting depth should be 2 / 3 of the cutting length. Then carry out maintenance according to the conventional method.

[0011] Furthermore, the matrix is ​​composed of lime soil, vermiculite and peat soil mixed in a mass ratio of 2:1:1; the pH of the cutting matrix is ​​5.5-6.

[0012] Furthermore, the water-fertilizer regulating particles in step (3) include the following raw materials:

[0013] Sodium alginate, 5wt% zinc chloride solution, 5wt% calcium chloride solution, potassium metaphosphate, 3,4-dimethylbenzaldehyde, citric acid, sodium bicarbonate, calcium peroxide, betaine, 150mg / L NAA solution.

[0014] Furthermore, the preparation method of the water-fertilizer regulating particles is as follows:

[0015] A: Add betaine to 100 times the mass of water to dissolve to obtain a betaine solution, then add 150 mg / L NAA solution and 3,4-dimethylbenzaldehyde, mix well to obtain a mixed solution, and set aside;

[0016] B: Dissolve sodium alginate in water to prepare a 10wt% sodium alginate solution, heat to 40-50°C, add potassium metaphosphate, stir and react for 10-20min, then add calcium peroxide and the mixture, stir and mix evenly, add citric acid and sodium bicarbonate, continue stirring for 1-2min, inject into 5wt% calcium chloride solution for gelation, let stand for 4-6h, remove and dry to obtain inner layer particles; set aside;

[0017] C: Sodium alginate is dissolved in water to prepare a 5wt% sodium alginate solution, added to the mixed solution and the inner layer particles, stirred and mixed evenly, and then injected into a 5wt% zinc chloride solution for gelation. After standing for 4 to 6 hours, the solution is removed and dried at 45°C for 30 minutes to obtain water-fertilizer regulating particles.

[0018] The water-fertilizer regulating particles prepared by the present invention are mixed into a matrix, and the water-fertilizer regulating particles can promote the absorption of nutrients and water by rock bean cuttings in a long-term manner, thereby ensuring the survival rate of the cuttings. Specifically, the water-fertilizer regulating particles are made of sodium alginate gel as an embedding material to embed a mixed solution made of NAA, betaine and 3,4-dimethylbenzaldehyde, and the NAA in the mixed solution is continuously released to act on the cuttings, thereby promoting the rooting of the cuttings, shortening the rooting time, and improving the survival rate of the cuttings; at the same time, the embedded 3,4-dimethylbenzaldehyde is released, reacts and combines with the cell membrane of the cuttings, changes the orderly arrangement of the molecular structure of the cell membrane, increases the intercellular gap, thereby enhancing the permeability of the substance, and then the betaine efficiently enters the cell membrane, promotes the absorption and utilization of nutrients and water by the cuttings by regulating the osmotic pressure of the cutting cells, thereby ensuring the physiological metabolism of the cuttings, and improving the survival rate of the cuttings.

[0019] Directly inserting the cuttings into the block gel will reduce the permeability of oxygen and water, which may easily cause the cuttings to rot. Therefore, preparing it into smaller particles and mixing it into the matrix can ensure good air permeability. However, after being made into smaller particles, the mass transfer resistance of the embedded components is reduced, and they will be quickly released in a short time, resulting in insufficient action time, which makes it difficult to meet the 3-month new root growth time of rock-growing red beans. Therefore, the present invention uses sodium alginate and calcium chloride to react to prepare inner layer particles, and adds potassium metaphosphate during the preparation process. By adjusting the charge density in the sodium alginate solution, the interaction of sodium alginate during cross-linking is promoted, thereby preparing a three-dimensional network structure with higher cross-linking density and more complex and stable structure, improving the structural stability of the inner layer particles, enhancing the mass transfer resistance to the release of components in the mixed solution, and extending the sustained release time of the components in the mixed solution, thereby better meeting the slow rooting characteristics of rock-growing red beans. Furthermore, sodium alginate and zinc chloride are used for gelation and then the inner layer particles are embedded to prepare water-fertilizer regulating particles. Since rock-growing red beans are calciphilic plants, the present invention uses lime soil as the main matrix component. There are also high amounts of calcium ions. After the water-fertilizer regulating particles enter the matrix, the gel prepared by the outer layer of sodium alginate and zinc chloride undergoes ion exchange reaction with the high amount of calcium ions in the matrix, thereby accelerating the disintegration of the outer layer of gel structure and promptly releasing the components in the mixed solution embedded in the outer layer of sodium alginate gel. After a large amount of the mixed solution components embedded in the outer layer of gel are released, the inner layer particles with a stable structure also release the embedded mixed solution components relatively slowly, thereby maintaining a large supply of components in the mixed solution at the initial stage of cuttings, and ensuring that the water-fertilizer regulating particles can continuously act on the cuttings for a long period of time, thereby promoting the absorption and utilization of nutrients and water by the cuttings, while continuously promoting rooting, shortening the rooting time of the cuttings, and improving the efficiency of cutting propagation.

[0020] In addition, after the cuttings are implanted, they need to undergo a period of tissue healing before they begin to take root. During the tissue healing period, a higher amount of water is required to promote healing, while during the rooting period, a lower amount of water is required. Otherwise, the rooting will be inhibited due to excessive water content, and even the cuttings will rot. Therefore, when preparing the water-fertilizer regulating particles, the present invention also mixes the pore-forming agent sodium bicarbonate, citric acid and calcium peroxide into the inner layer particles. The sodium bicarbonate and citric acid react to release gas, thereby forming a porous structure inside the gel, thereby improving the water permeability of the inner layer particles; and the outer layer gel structure has a relatively loose three-dimensional network structure. Dispersed, it can retain more water, and then after the water-fertilizer particles enter the matrix, the early outer gel structure has high water retention, which can ensure that the early matrix soil contains more water to promote tissue healing. When the outer gel structure decomposes, the inner particles have higher water permeability, and then have lower water retention. In the middle and late stages, it can reduce the water content in the matrix, thereby promoting rooting and preventing the roots from being inhibited or even rotten due to excessive water. By using water-fertilizer regulating particles to promote tissue healing, rooting, and improve the survival rate of cuttings, the efficiency of rock bean cutting propagation can be effectively improved.

[0021] Furthermore, in step A, the mass ratio of the betaine solution, the NAA solution, and the 3,4-dimethylbenzaldehyde is (0.6-1): (1-2): (0.05-0.06).

[0022] Furthermore, in step B, the mass ratio of the sodium alginate solution, potassium metaphosphate, calcium peroxide, mixed solution, citric acid, and sodium bicarbonate is (10-15): (0.1-0.15): (0.2-0.3): (1-2): (0.05-0.1): (0.05-0.1).

[0023] Furthermore, in step C, the mass ratio of the sodium alginate solution, the mixed solution, and the inner layer particles is (10-15): (0.5-1): (2-3).

[0024] Furthermore, in step B, the inner layer particles are dried to a moisture content of 15-20%.

[0025] Furthermore, in step (3), the mass ratio of the substrate to the water-fertilizer regulating particles is (3-5):1.

[0026] Beneficial effects:

[0027] 1. The present invention prepares water-fertilizer regulating particles and mixes them into the rock bean cutting matrix to continuously promote the absorption and utilization of nutrients and water by the cuttings, thereby ensuring the supply of nutrients and water during the period when new roots of the cuttings have not grown, and improving the survival rate of the rock bean cuttings.

[0028] 2. When preparing the water-fertilizer regulating granules, the present invention uses sodium alginate gel to react with zinc chloride and calcium chloride to form gels with different cross-linking degrees, thereby having different sustained-release properties, wherein the outer layer gel can release the embedded components in a high calcium content matrix in a timely manner, and the inner layer granules slowly release the embedded components, thereby ensuring the release amount while ensuring the sustained action time of the water-fertilizer regulating granules, thereby better improving the propagation efficiency of rock bean cuttings. DETAILED DESCRIPTION

[0029] The present invention will be described in detail below with reference to specific embodiments:

[0030] Example 1: Preparation of water and fertilizer regulation particles

[0031] A: Dissolve betaine in 100 times the mass of water to prepare a betaine solution. Add 1.5 kg of 150 mg / L NAA solution and 0.05 kg of 3,4-dimethylbenzaldehyde to 0.7 kg of the betaine solution, mix well to obtain a mixed solution, and set aside.

[0032] B: Sodium alginate is dissolved in water to prepare 12kg of 10wt% sodium alginate solution, and then 0.12kg of potassium metaphosphate is added after heating to 45°C and stirred for 15min, and then 0.25kg of calcium peroxide and 1.5kg of the mixed solution are added and stirred to mix evenly, and then 0.06kg of citric acid and 0.06kg of sodium bicarbonate are added and stirred for 1min, and then slowly injected into 5wt% calcium chloride solution for gelation to prepare gel particles, and after standing for 5h, the inner layer particles are removed and dried at 60°C until the moisture content is about 15% to obtain inner layer particles; and set aside;

[0033] C: Dissolve sodium alginate in water to prepare 12 kg of 5 wt% sodium alginate solution, add 0.6 kg of the mixed solution and 2 kg of inner layer particles, stir and mix evenly, then slowly inject into 5 wt% zinc chloride solution for gelation, let stand for 5 hours and dry at 45°C for 30 minutes to obtain water-fertilizer regulating particles.

[0034] Example 2: Preparation of water and fertilizer regulation particles

[0035] A: Dissolve betaine in 100 times the mass of water to prepare a betaine solution. Add 1 kg of 150 mg / L NAA solution and 0.05 kg of 3,4-dimethylbenzaldehyde to 0.6 kg of the betaine solution, mix well to obtain a mixed solution, and set aside.

[0036] B: Sodium alginate is dissolved in water to prepare 10 kg of 10 wt% sodium alginate solution, and then 0.1 kg of potassium metaphosphate is added after heating to 40°C and stirred for 20 minutes, and then 0.2 kg of calcium peroxide and 1 kg of the mixed solution are added and stirred to mix evenly, and then 0.05 kg of citric acid and 0.05 kg of sodium bicarbonate are added and stirred for 1 minute, and then 5 wt% calcium chloride solution is slowly injected for gelation, and then allowed to stand for 4 hours and then dried at 60°C until the moisture content is about 15% to obtain inner layer particles; set aside;

[0037] C: Dissolve sodium alginate in water to prepare 10 kg of 5 wt% sodium alginate solution, add 0.5 kg of the mixed solution and 2 kg of inner layer particles, stir and mix evenly, then slowly inject into 5 wt% zinc chloride solution for gelation, let stand for 4 hours and dry at 45°C for 30 minutes to obtain water-fertilizer regulating particles.

[0038] Example 3: Preparation of water and fertilizer regulation particles

[0039] A: Dissolve betaine in 100 times the mass of water to prepare a betaine solution. Add 2 kg of 150 mg / L NAA solution and 0.06 kg of 3,4-dimethylbenzaldehyde to 1 kg of the betaine solution, mix well to obtain a mixed solution, and set aside.

[0040] B: Sodium alginate is dissolved in water to prepare 15kg of 10wt% sodium alginate solution, and then 0.15kg of potassium metaphosphate is added after heating to 50°C and stirred for 20min, and then 0.3kg of calcium peroxide and 2kg of the mixed solution are added and stirred to mix evenly, and then 0.1kg of citric acid and 0.1kg of sodium bicarbonate are added and stirred for 1min, and then 5wt% calcium chloride solution is slowly injected for gelation, and then allowed to stand for 6h and dried at 60°C until the moisture content is 15% to obtain inner layer particles; set aside;

[0041] C: Sodium alginate was dissolved in water to prepare 15 kg of 5 wt% sodium alginate solution, 1 kg of the mixed solution and 3 kg of inner layer particles were added, and after stirring and mixing evenly, 5 wt% zinc chloride solution was slowly injected for gelation. After standing for 6 hours, it was dried at 45°C for 30 minutes to obtain water-fertilizer regulating particles.

[0042] Comparative Example 1: Preparation of water and fertilizer regulation particles

[0043] In contrast to Example 1, the only difference is that in the preparation of the water-fertilizer regulating particles in Comparative Example 1, 3,4-dimethylbenzaldehyde is not added during the preparation of the mixed solution in step A, as shown below:

[0044] A: Dissolve betaine in 100 times the mass of water to prepare a betaine solution. Add 1.5 kg of 150 mg / L NAA solution to 0.7 kg of the betaine solution, mix well to obtain a mixed solution, and set aside.

[0045] B~C: Same as Example 1.

[0046] Comparative Example 2: Preparation of water and fertilizer regulation particles

[0047] In contrast to Example 1, the only difference is that in the preparation of the water-fertilizer regulating particles in Comparative Example 2, betaine is not added in step A, but clean water is directly added, as shown below:

[0048] A: Add 1.5 kg of 150 mg / L NAA solution and 0.05 kg of 3,4-dimethylbenzaldehyde to 0.7 kg of clean water, mix well to obtain a mixed solution, and set aside for use;

[0049] B~C: Same as Example 1.

[0050] Comparative Example 3: Preparation of water and fertilizer regulation particles

[0051] In contrast to Example 1, the only difference is that in the preparation of the water-fertilizer regulating particles in Comparative Example 3, potassium metaphosphate is not added in step B for reaction, as shown below:

[0052] A: Same as Example 1;

[0053] B: Dissolve sodium alginate in water to prepare 12kg of 10wt% sodium alginate solution, add 0.25kg of calcium peroxide and 1.5kg of the mixed solution and stir to mix evenly, then add 0.06kg of citric acid and 0.06kg of sodium bicarbonate and continue stirring for 1min, then slowly inject into 5wt% calcium chloride solution for gelation to prepare gel particles, let stand for 5h, then take out and dry at 60°C until the moisture content is about 15% to obtain inner layer particles; set aside;

[0054] C: Same as Example 1

[0055] Comparative Example 4: Preparation of water and fertilizer regulation particles

[0056] In contrast to Example 1, the only difference is that in Comparative Example 4, step B is missing when preparing the water-fertilizer regulating granules, that is, the inner layer granules are not prepared, and the mixed solution is added at one time to prepare the water-fertilizer regulating granules, as shown below:

[0057] A: Same as Example 1;

[0058] B: Sodium alginate was dissolved in water to prepare 12 kg of 5 wt% sodium alginate solution, 2.1 kg of the mixed solution was added, and after stirring and mixing evenly, 5 wt% zinc chloride solution was slowly injected for gelation. After standing for 5 hours, it was dried at 45°C for 30 minutes to obtain water-fertilizer regulating particles.

[0059] Comparative Example 5: Preparation of Moisture Controlled Granules

[0060] In contrast to Example 1, the only difference is that calcium peroxide is not added in step B when preparing the water-fertilizer regulating particles in Comparative Example 5, as shown below:

[0061] A: Same as Example 1;

[0062] B: Sodium alginate is dissolved in water to prepare 12kg of 10wt% sodium alginate solution, and then 0.12kg of potassium metaphosphate is added after heating to 45°C and stirred for 15min, and then 1.5kg of the mixed solution is added and stirred to mix evenly, and then 0.06kg of citric acid and 0.06kg of sodium bicarbonate are added and stirred for 1min, and then slowly injected into 5wt% calcium chloride solution for gelation to prepare gel particles, and after standing for 5h, the inner layer particles are obtained after being taken out and dried at 60°C until the moisture content is about 15%; and set aside;

[0063] C: Same as Example 1.

[0064] Comparative Example 6: Preparation of water and fertilizer regulation particles

[0065] In contrast to Example 1, the only difference is that in the preparation of the water-fertilizer regulating particles in Comparative Example 6, citric acid and sodium bicarbonate are not added in step B, as shown below:

[0066] A: Same as Example 1;

[0067] B: Sodium alginate is dissolved in water to prepare 12kg of 10wt% sodium alginate solution, and then 0.12kg of potassium metaphosphate is added after heating to 45°C and stirred for 15min, and then 0.25kg of calcium peroxide and 1.5kg of the mixed solution are added and stirred to mix evenly, and then 5wt% calcium chloride solution is slowly injected to gel to prepare gel particles, and after standing for 5h, the solution is fished out and dried at 60°C until the moisture content is about 15% to obtain inner layer particles; and set aside;

[0068] C: Same as Example 1.

[0069] Comparative Example 7: Preparation of water and fertilizer regulation particles

[0070] In contrast to Example 1, the only difference is that in Comparative Example 7, step C is missing when preparing the water-fertilizer regulating granules, that is, the inner layer granules are directly prepared to obtain the water-fertilizer regulating granules, as shown below:

[0071] A: Same as Example 1;

[0072] B: Sodium alginate is dissolved in water to prepare 12kg of 10wt% sodium alginate solution, which is heated to 45°C and then added with 0.12kg of potassium metaphosphate and stirred for 15min, followed by adding 0.25kg of calcium peroxide and 1.5kg of the mixed solution and stirred to mix evenly, followed by adding 0.06kg of citric acid and 0.06kg of sodium bicarbonate and stirring for 1min, followed by slowly injecting into 5wt% calcium chloride solution for gelation to prepare gel particles, which are allowed to stand for 5h and then removed and dried at 60°C until the moisture content is about 15% to obtain water-fertilizer regulating particles.

[0073] Embodiment 4: rock-growing red bean cutting propagation method

[0074] (1) Cutting collection: In mid-May, one-year-old lignified rock bean branches that are healthy and free of pests and diseases are collected from healthy mother trees. After the branches are collected, they are cut into sections of about 15 cm, the upper ends are cut flat and the lower ends are cut obliquely, and 3 to 4 buds are retained to obtain cuttings;

[0075] (2) Pretreatment of cuttings: The cuttings were immersed in a 0.1 wt% potassium permanganate solution for 15 min for disinfection, and then washed with water for 3 times. Then, the lower end of the cuttings was immersed in a 300 mg / L NAA solution for 1 h to obtain pretreated cuttings.

[0076] (3) Cutting: lime soil, vermiculite and peat soil are mixed in a mass ratio of 2:1:1 to obtain a matrix, the pH of the matrix is ​​adjusted to 5.8, the matrix and the water-fertilizer regulating particles prepared by the method of Example 1 are mixed in a mass ratio of 4:1 and then placed in a seedling pot, and then the cuttings are inserted into the matrix. The cutting depth is 2 / 3 of the cutting length, and then the conventional method is used for maintenance.

[0077] Experiment: Cutting experiment of red bean

[0078] 1. The effects of the water-fertilizer regulating particles prepared in Example 1 and Comparative Examples 1 to 7 were tested. The experiment was conducted in a botanical garden in Guizhou Province. The experiment was divided into 7 groups: experimental group 1, control groups 1 to 7, and a blank control group. The water-fertilizer regulating particles and cutting methods used in each group were as follows:

[0079] Experimental group 1: using the water-fertilizer regulating particles prepared in Example 1 and the cutting method of Example 4;

[0080] Control groups 1 to 7: using the water-fertilizer regulating particles of comparative examples 1 to 7 and the cutting method of embodiment 4 respectively;

[0081] Blank control group: no water-fertilizer regulating particles were added, and the remaining method steps were the same as those in Example 4.

[0082] 2. Each group of rock bean cuttings had 15 trees, which were managed and maintained in the same way. Then, the water content of the substrate in each group was tested 10 days and 30 days after cutting, and the rooting time of each group and the survival rate of the cuttings after 120 days were recorded. The experiment was repeated three times, and the data were shown in Table 1:

[0083] Table 1

[0084]

[0085]

[0086] According to the data analysis in Table 1:

[0087] (1) According to the method of the present invention, water-fertilizer regulating particles are prepared and mixed into the matrix for cutting of rock bean, which can effectively shorten the rooting time of rock bean cutting and improve the survival rate of cutting. The rooting time can be shortened to 73 days, while the rooting time of the blank control group without adding water-fertilizer regulating particles is 86 days. In addition, the survival rate of rock bean cutting of the present invention can reach 84.4%, which is 22.2% higher than that of the blank control group without adding water-fertilizer regulating particles. The method of the present invention can effectively improve the survival rate of cutting of rock bean.

[0088] (2) In the control group 1, 3,4-dimethylbenzaldehyde was not added during the preparation of the water-fertilizer regulating granules, resulting in limited absorption of the components of the mixed solution embedded in the water-fertilizer granules by the cuttings, and thus limited absorption of water and nutrients, which prolonged the rooting time and reduced the survival rate. In the control group 2, betaine was not added during the preparation of the water-fertilizer regulating granules, and the cell osmotic regulation ability was low, and the absorption of nutrients and water was poor, resulting in a significant increase in the rooting time and a decrease in the survival rate. In the control group 3, potassium metaphosphate was not added during the preparation of the water-fertilizer regulating granules, and the inner layer of the granules had a low cross-linking density and poor stability, which resulted in too fast release of the embedded components, resulting in no effect in the later stage and a reduced survival rate.

[0089] (3) In the control group 4, the inner layer of the water-fertilizer granules was not prepared when the water-fertilizer granules were prepared. Only the mixture was encapsulated by the reaction of sodium alginate and zinc chloride to prepare the water-fertilizer regulating granules. After entering the matrix soil, it decomposed quickly, the continuous action time was short, and the survival rate of the cuttings was significantly reduced; in the control group 5, calcium peroxide was not added when the water-fertilizer regulating granules were prepared, and the oxygen content in the matrix was low, which affected the rooting time and survival rate of the cuttings; in the control group 6, citric acid and sodium bicarbonate were not added when the water-fertilizer granules were prepared, and the inner layer of the granules had low water permeability, which in turn affected the rooting of the cuttings. In addition, the reaction products of citric acid and sodium bicarbonate also promoted the growth of the cuttings. As a result, the rooting time and survival rate of the cuttings in the experimental group 1 were relatively ideal; in the control group 7, the water-fertilizer regulating granules were prepared only from the inner layer of the granules. The water retention of the inner layer of the granules was low, the water content of the matrix was low in the early stage, and the components of the encapsulated mixture were released slowly, which affected tissue healing and thus affected the rooting time and survival rate.

[0090] The above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the purpose and scope of the technical solutions of the present invention, which should be included in the scope of the claims of the present invention. The techniques, shapes, and structural parts not described in detail in the present invention are all known technologies.

Claims

1. A method for propagating rock bean by cuttings, characterized in that: The method is as follows: (1) Cutting collection: From April to June, collect one-year-old lignified rock bean branches from healthy mother trees. After the branches are collected, cut them into 10-20 cm segments and retain 3-4 buds to obtain cuttings; (2) Pretreatment of cuttings: The cuttings were immersed in a 0.1 wt% potassium permanganate solution for disinfection for 10 to 15 minutes, and then washed with water after disinfection. Then, the lower end of the cuttings was immersed in a 300 mg / L NAA solution for 1 to 2 hours to obtain pretreated cuttings; (3) Cutting: Mix the substrate and water-fertilizer regulating particles evenly and put them into the seedling pot. Then insert the pretreated cuttings into the substrate. The cutting depth should be 2 / 3 of the cutting length. Then carry out maintenance according to the conventional method.

2. A rock bean cutting propagation method according to claim 1, characterized in that: The matrix is ​​composed of lime soil, vermiculite and peat soil mixed in a mass ratio of 2:1:1; the pH value of the cutting matrix is ​​5.5-6.

3. A rock-growing red bean cutting propagation method according to claim 2, characterized in that, The water-fertilizer regulating particles in step (3) include the following raw materials: Sodium alginate, 5wt% zinc chloride solution, 5wt% calcium chloride solution, potassium metaphosphate, 3,4-dimethylbenzaldehyde, citric acid, sodium bicarbonate, calcium peroxide, betaine, 150mg / L NAA solution.

4. A rock-growing red bean cutting propagation method according to claim 3, characterized in that: The preparation method of the water-fertilizer regulating granules is as follows: A: Add betaine to 100 times the mass of water to dissolve to obtain a betaine solution, then add 150 mg / L NAA solution and 3,4-dimethylbenzaldehyde, mix well to obtain a mixed solution, and set aside; B: Dissolve sodium alginate in water to prepare a 10wt% sodium alginate solution, heat to 40-50°C, add potassium metaphosphate, stir and react for 10-20min, then add calcium peroxide and the mixture, stir and mix evenly, add citric acid and sodium bicarbonate, continue stirring for 1-2min, inject into 5wt% calcium chloride solution for gelation, let stand for 4-6h, remove and dry to obtain inner layer particles; set aside; C: Sodium alginate is dissolved in water to prepare a 5wt% sodium alginate solution, added to the mixed solution and the inner layer particles, stirred and mixed evenly, and then injected into a 5wt% zinc chloride solution for gelation. After standing for 4 to 6 hours, the solution is removed and dried at 45°C for 30 minutes to obtain water-fertilizer regulating particles.

5. A rock-growing red bean cutting propagation method according to claim 4, characterized in that: The mass ratio of the betaine solution, the NAA solution and the 3,4-dimethylbenzaldehyde in the step A is (0.6-1): (1-2): (0.05-0.06).

6. A rock-growing red bean cutting propagation method according to claim 5, characterized in that: In the step B, the mass ratio of the sodium alginate solution, potassium metaphosphate, calcium peroxide, the mixed solution, citric acid and sodium bicarbonate is (10-15): (0.1-0.15): (0.2-0.3): (1-2): (0.05-0.1): (0.05-0.1).

7. A rock-growing red bean cutting propagation method according to claim 6, characterized in that: In the step C, the mass ratio of the sodium alginate solution, the mixed solution and the inner layer particles is (10-15): (0.5-1): (2-3).

8. A rock-growing red bean cutting propagation method according to claim 7, characterized in that: In the step B, the inner layer particles are dried to a moisture content of 15-20%.

9. A rock-growing red bean cutting propagation method according to claim 8, characterized in that: The mass ratio of the substrate to the water-fertilizer regulating particles in the step (3) is (3-5):1.

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