Method for antenatal rearing and postpartum care of seriola aureovittata parent fish

Abstract

The invention discloses a seriola aureovittata parent fish feed containing Chinese herbal medicines and a seriola aureovittata breeding method using the feed, and different raw materials are matched, so that the seriola aureovittata parent fish feed can better provide nutritional ingredients and active substances for seriola aureovittata parent fish. The feed can enhance the physical ability and immunocompetence of the parent fish before delivery, promote the development of the parent fish, accelerate sexual maturity and improve the induced spawning rate, egg laying amount, fertilization rate and hatching rate, and can promote the healing and physique recovery of the parent fish after delivery, thereby ensuring the survival rate and quality of the parent fish after delivery.

Description

Technical Field

[0001] The invention relates to the technical field of aquaculture, and in particular to a method for prenatal cultivation and postnatal care of greater amberjack broodstock. Background Art

[0002] The greater amberjack (Seriola dumerili) belongs to the genus Seriola of the family Seriola in the order Perciformes. It is a large warm-temperate fish that lives in the upper and middle layers of the ocean. It is widely distributed in tropical and subtropical waters of all oceans and is also one of the precious fish species in the South my country Sea. It grows fast, has tender meat, and tastes delicious. It is rich in protein, linolenic acid, linoleic acid and other essential fatty acids for the human body, and highly unsaturated fatty acids (EPA, DHA). It is also an excellent fish for making sashimi, and commercial breeding has been carried out in many places. The first sexual maturity age of the greater amberjack is 3-4 years old. Although there have been breakthroughs in the artificial breeding technology of the greater amberjack, its induced spawning rate, egg production, fertilization rate and hatching rate are still relatively low. The quality of broodstock has a decisive material basis in the process of artificial breeding of fish. Only good broodstock can ensure that the bred fry are of high quality, so the cultivation of broodstock is crucial.

[0003] How to improve the spawning rate, egg production, fertilization rate and hatching rate of greater amberjack and reduce the postpartum mortality of greater amberjack broodstock is one of the key technologies to solve the large-scale artificial breeding of greater amberjack. Therefore, doing a good job in prenatal cultivation and postnatal care of greater amberjack broodstock is the key to ensuring the growth of aquaculture production, and is also of great significance to the sustainable development of the greater amberjack industry. Summary of the invention

[0004] A first aspect of the present invention provides a greater amberjack broodstock feed, wherein the raw materials for preparing the feed include trash fish, Antarctic krill, eel meal, flour, multivitamins, Chinese herbal medicine and probiotic bacterial liquid.

[0005] Chinese herbal medicine is a natural green feed additive, rich in bioactive substances and nutrients, and has the effect of improving animal digestion performance, growth performance and non-specific immune function. Chinese herbal medicine is not easy to produce drug resistance due to its unique anti-microbial and anti-parasitic function and mechanism of action. The present invention adds Chinese herbal medicine to the feed of greater amberjack broodstock, so that it can better provide nutrients and active substances for greater amberjack broodstock.

[0006] In some embodiments, the feed preparation raw materials further include oysters and fish oil, and the Chinese herbal medicines include hawthorn, Ligustrum lucidum, Salvia miltiorrhiza, Cyperus rotundus, Ligusticum chuanxiong and Astragalus membranaceus.

[0007] Oysters and fish oil are further added to the raw materials for preparing the feed, and Chinese herbal medicines including hawthorn, glossy privet, salvia miltiorrhiza, cyrtomium, ligusticum and astragalus are added. The prepared feed is more suitable for pre-natal intensive cultivation of greater amberjack broodstock, enhances the physical fitness and immune ability of the broodstock, promotes the development of the broodstock, accelerates sexual maturity, and increases the rate of induction of labor, egg production, fertilization rate and hatching rate.

[0008] In some embodiments, the probiotic bacterial liquid comprises Bacillus subtilis and Lactobacillus.

[0009] In some embodiments, the feed preparation raw materials include 50-60 parts of trash fish, 14-16 parts of Antarctic krill, 14-16 parts of oysters, 9-11 parts of eel meal, 2-4 parts of fish oil, 1-5 parts of flour, 0.1-1 parts of multivitamins, 0.1-1 parts of Chinese herbal medicine, and 1-3 parts of probiotics liquid, and the concentration of the liquid is 1×10 6 -3×10 6 cfu / ml.

[0010] In some embodiments, the raw materials for preparing the feed include, by weight, 55 parts of miscellaneous fish, 14 parts of Antarctic krill, 14 parts of oysters, 10 parts of eel meal, 2 parts of fish oil, 4 parts of flour, 0.5 parts of multivitamins, 0.5 parts of Chinese herbal medicine, and 2 parts of probiotic liquid.

[0011] In some embodiments, the raw materials for preparing the feed include, by weight, 52 parts of miscellaneous fish, 15 parts of Antarctic krill, 15 parts of oysters, 10 parts of eel meal, 4 parts of fish oil, 3 parts of flour, 0.5 parts of multivitamins, 0.5 parts of Chinese herbal medicine, and 2 parts of probiotic liquid.

[0012] In some embodiments, the mass ratio of Crataegus pinnatifida, Fructus Ligustri Lucidi, Salvia miltiorrhiza, Rhizoma Cyrthospermi, Rhizoma Chuanxiong and Radix Astragali in the Chinese herbal medicine is 1:1:1:1:1:1:1.

[0013] In some embodiments, the probiotic bacterial solution comprises a Bacillus subtilis bacterial solution and a Lactobacillus bacterial solution, the volume ratio of the two bacterial solutions is 1:1, and the concentration of the two bacterial solutions is 1×10 6 -3×10 6 cfu / ml, preferably about 2×10 6 cfu / ml.

[0014] In some embodiments, the feed is a soft pellet feed.

[0015] Soft pellet feed is a green and environmentally friendly aquatic feed. During the production process, it can not only produce a large number of beneficial bacteria and metabolites, but also improve the nutritional composition and palatability of the feed, promote the digestion and absorption of nutrients by the greater amberjack, and improve the feed conversion efficiency. It can also inhibit the reproduction of harmful microorganisms, thereby improving the production performance and disease resistance of the greater amberjack.

[0016] In some embodiments, the soft pellet feed has a particle size of 1.4-1.6 cm and a length of 4-6 cm.

[0017] In some embodiments, the trash fish comprises mackerel and triggerfish.

[0018] In some embodiments, the method for preparing the feed comprises:

[0019] Crush the miscellaneous fish, Antarctic krill, and oysters separately and mix them;

[0020] The mixture is placed in a fermentation tank, and the probiotic solution is added to the fermentation tank and stirred evenly with the mixture. The temperature of the fermentation tank is controlled at 35-40°C and fermented for 48-72 hours;

[0021] Mix the fermented mixture with eel powder, fish oil, flour, multivitamins and Chinese herbal medicine powder;

[0022] The feed is extruded into soft pellets with a particle size of 1.4-1.6 cm and a length of 4-6 cm by a pelletizer.

[0023] In some embodiments, the raw materials for preparing the feed further include sandworms, squid paste and complex amino acids, and the Chinese herbal medicines include hawthorn, cyrtomium, astragalus, honeysuckle, licorice and codonopsis.

[0024] Sandworms, squid paste and complex amino acids are further added to the raw materials for preparing the feed, and Chinese herbal medicines including hawthorn, cyperus, astragalus, honeysuckle, licorice and codonopsis are added. The prepared feed is more suitable for increasing the nutritional supply of postpartum broodstock, can promote the broodstock to recover their physical fitness, and ensure the survival rate and quality of the broodstock after delivery.

[0025] In some embodiments, the probiotic bacterial liquid comprises Bacillus subtilis and Lactobacillus.

[0026] In some embodiments, the feed preparation raw materials include 55-65 parts of trash fish, 14-16 parts of Antarctic krill, 9-11 parts of sandworms, 9-11 parts of eel powder, 2-4 parts of squid paste, 1-5 parts of flour, 0.1-1 parts of complex vitamins, 0.1-1 parts of complex amino acids, 0.1-1 parts of Chinese herbal medicine, and 1-3 parts of probiotics liquid, and the concentration of the liquid is 1×10 6 -3×10 6 cfu / ml.

[0027] In some embodiments, the raw materials for preparing the feed include, by mass, 60 parts of miscellaneous fish, 14 parts of Antarctic krill, 9 parts of sandworms, 10 parts of eel meal, 2 parts of squid paste, 3 parts of flour, 0.5 parts of complex vitamins, 0.5 parts of complex amino acids, 1 part of Chinese herbal medicine, and 2 parts of probiotic liquid.

[0028] In some embodiments, the raw materials for preparing the feed include, by mass, 57 parts of miscellaneous fish, 15 parts of Antarctic krill, 10 parts of sandworms, 10 parts of eel meal, 3 parts of squid paste, 3 parts of flour, 0.5 parts of complex vitamins, 0.5 parts of complex amino acids, 1 part of Chinese herbal medicine, and 2 parts of probiotic liquid.

[0029] In some embodiments, the mass ratio of hawthorn, cyrtomium, astragalus, honeysuckle, licorice, and codonopsis in the Chinese herbal medicine is 1:1:1:1:1:1:1.

[0030] In some embodiments, the probiotic bacterial solution comprises a Bacillus subtilis bacterial solution and a Lactobacillus bacterial solution, the volume ratio of the two bacterial solutions is 1:1, and the concentration of the two bacterial solutions is 1×10 6 -3×10 6 cfu / ml, preferably about 2×10 6 cfu / ml.

[0031] In some embodiments, the feed is a soft pellet feed.

[0032] In some embodiments, the soft pellet feed has a particle size of 1.4-1.6 cm and a length of 4-6 cm.

[0033] In some embodiments, the trash fish comprises mackerel and triggerfish.

[0034] In some embodiments, the method for preparing the feed comprises:

[0035] Crush the miscellaneous fish, Antarctic krill and lugworms separately and mix them;

[0036] The mixture is placed in a fermentation tank, and the probiotic solution is added to the fermentation tank and stirred evenly with the mixture. The temperature of the fermentation tank is controlled at 35-40°C and fermented for 48-72 hours;

[0037] The fermented mixture is mixed evenly with eel powder, squid paste, flour, complex vitamins, complex amino acids and Chinese herbal medicine powder, and extruded into soft granular feed with a particle size of 1.4-1.6cm and a length of 4-6cm by a granulator.

[0038] The second aspect of the present invention provides a reagent combination for artificial breeding of greater amberjack, comprising oxytocin, an anti-inflammatory composition and greater amberjack broodstock feed; the greater amberjack broodstock feed includes greater amberjack broodstock prenatal intensive cultivation feed and greater amberjack broodstock postnatal care feed, and the raw materials for preparing the greater amberjack broodstock feed include miscellaneous fish, Antarctic krill, eel meal, flour, multivitamins, Chinese herbal medicine and probiotic bacterial liquid. Among them, oxytocin is used for inducing labor of greater amberjack broodstock, and the anti-inflammatory composition is used for anti-inflammatory treatment of greater amberjack broodstock after delivery.

[0039] In some embodiments, the oxytocin comprises a gonadotropin-releasing hormone agonist, dioconazole, and an extract of the pituitary gland of Epinephelus coioides.

[0040] In some embodiments, the coioidal grouper is a 3-4 year old coioidal grouper, preferably a 3 year old coioidal grouper.

[0041] In some embodiments, the anti-inflammatory composition comprises:

[0042] (a) dextrose and doxycycline hydrochloride; and / or

[0043] (b) Glucose, penicillin and complex amino acids.

[0044] In some embodiments, the anti-inflammatory composition comprises glucose and doxycycline hydrochloride, and the anti-inflammatory composition is used for reducing inflammation in broodstock with no damage on the body surface.

[0045] In some embodiments, the anti-inflammatory composition comprises glucose, penicillin and complex amino acids; the anti-inflammatory composition is used to reduce inflammation in severely injured broodstock (i.e., those with obvious body surface injuries).

[0046] In some embodiments, the raw materials for preparing the pre-natal enhanced breeding feed for greater amberjack broodstock include miscellaneous fish, Antarctic krill, eel meal, flour, multivitamins, Chinese herbal medicine, probiotic liquid, oysters and fish oil, and the Chinese herbal medicine includes hawthorn, Ligustrum lucidum, Salvia miltiorrhiza, Cynomorium fortunei, Ligusticum chuanxiong and Astragalus membranaceus.

[0047] In some embodiments, the probiotic bacterial liquid comprises Bacillus subtilis and Lactobacillus.

[0048] In some embodiments, the raw materials for preparing the pre-natal enhanced cultivation feed for greater amberjack broodstock include 50-60 parts of miscellaneous fish, 14-16 parts of Antarctic krill, 14-16 parts of oysters, 9-11 parts of eel meal, 2-4 parts of fish oil, 1-5 parts of flour, 0.1-1 parts of multivitamins, 0.1-1 parts of Chinese herbal medicine, and 1-3 parts of probiotic bacterial liquid, and the concentration of the bacterial liquid is 1×10 6 -3×10 6 cfu / ml.

[0049] In some embodiments, the raw materials for preparing the pre-natal enhanced breeding feed for greater amberjack broodstock include, by weight, 55 parts of miscellaneous fish, 14 parts of Antarctic krill, 14 parts of oysters, 10 parts of eel meal, 2 parts of fish oil, 4 parts of flour, 0.5 parts of multivitamins, 0.5 parts of Chinese herbal medicine, and 2 parts of probiotic liquid.

[0050] In some embodiments, the raw materials for preparing the pre-natal enhanced breeding feed for greater amberjack broodstock include, by weight, 52 parts of miscellaneous fish, 15 parts of Antarctic krill, 15 parts of oysters, 10 parts of eel meal, 4 parts of fish oil, 3 parts of flour, 0.5 parts of multivitamins, 0.5 parts of Chinese herbal medicine, and 2 parts of probiotic liquid.

[0051] In some embodiments, the mass ratio of Crataegus pinnatifida, Fructus Ligustri Lucidi, Salvia miltiorrhiza, Rhizoma Cyrthospermi, Rhizoma Chuanxiong and Radix Astragali in the Chinese herbal medicine is 1:1:1:1:1:1:1.

[0052] In some embodiments, the probiotic bacterial solution comprises a Bacillus subtilis bacterial solution and a Lactobacillus bacterial solution, the volume ratio of the two bacterial solutions is 1:1, and the concentration of the two bacterial solutions is 1×10 6 -3×10 6 cfu / ml, preferably about 2×10 6 cfu / ml.

[0053] In some embodiments, the pre-spawning fortified cultivation feed for greater amberjack broodstock is a soft pellet feed.

[0054] In some embodiments, the soft pellet feed has a particle size of 1.4-1.6 cm and a length of 4-6 cm.

[0055] In some embodiments, the raw materials for preparing the greater amberjack broodstock postpartum care feed include miscellaneous fish, Antarctic krill, eel meal, flour, complex vitamins, Chinese herbal medicine, probiotic bacterial liquid, sandworms, squid paste and complex amino acids, and the Chinese herbal medicine includes hawthorn, cyrtomium, astragalus, honeysuckle, licorice and codonopsis.

[0056] In some embodiments, the probiotic bacterial liquid comprises Bacillus subtilis and Lactobacillus.

[0057] In some embodiments, the raw materials for preparing the greater amberjack broodstock postpartum care feed include 55-65 parts of miscellaneous fish, 14-16 parts of Antarctic krill, 9-11 parts of sandworms, 9-11 parts of eel powder, 2-4 parts of squid paste, 1-5 parts of flour, 0.1-1 parts of complex vitamins, 0.1-1 parts of complex amino acids, 0.1-1 parts of Chinese herbal medicine, and 1-3 parts of probiotic bacterial liquid, and the bacterial liquid concentration is 1×10 6 -3×10 6 cfu / ml.

[0058] In some embodiments, the raw materials for preparing the greater amberjack broodstock postpartum care feed include, by mass, 60 parts of miscellaneous fish, 14 parts of Antarctic krill, 9 parts of lugworms, 10 parts of eel meal, 2 parts of squid paste, 3 parts of flour, 0.5 parts of complex vitamins, 0.5 parts of complex amino acids, 1 part of Chinese herbal medicine, and 2 parts of probiotic liquid.

[0059] In some embodiments, the raw materials for preparing the greater amberjack broodstock postpartum care feed include, by mass, 57 parts of miscellaneous fish, 15 parts of Antarctic krill, 10 parts of lugworms, 10 parts of eel meal, 3 parts of squid paste, 3 parts of flour, 0.5 parts of complex vitamins, 0.5 parts of complex amino acids, 1 part of Chinese herbal medicine, and 2 parts of probiotic liquid.

[0060] In some embodiments, the mass ratio of hawthorn, cyrtomium, astragalus, honeysuckle, licorice, and codonopsis in the Chinese herbal medicine is 1:1:1:1:1:1:1.

[0061] In some embodiments, the probiotic bacterial solution comprises a Bacillus subtilis bacterial solution and a Lactobacillus bacterial solution, the volume ratio of the two bacterial solutions is 1:1, and the concentration of the two bacterial solutions is 1×10 6 -3×10 6 cfu / ml, preferably about 2×10 6 cfu / ml.

[0062] In some embodiments, the greater amberjack broodstock postpartum care feed is a soft pellet feed.

[0063] In some embodiments, the soft pellet feed has a particle size of 1.4-1.6 cm and a length of 4-6 cm.

[0064] The third aspect of the present invention provides use of the feed of the first aspect and the reagent combination of the second aspect in artificial breeding of greater amberjack.

[0065] A fourth aspect of the present invention provides a method for artificial breeding of greater amberjack, comprising the following steps:

[0066] Pre-spawning intensive cultivation of broodstock: Use pre-spawning intensive cultivation feed for greater amberjack broodstock to cultivate broodstock;

[0067] Secondary ovulation promotion: Secondary ovulation promotion is carried out on female broodstock that do not lay eggs or whose egg laying amount does not exceed 50%;

[0068] Postpartum care: After the greater amberjack broodstock have completed reproduction (reproduction completion means that all female broodstock have laid more than 50% of their eggs), the broodstock are raised using greater amberjack broodstock postpartum care feed;

[0069] The raw materials for preparing the pre-natal enhanced cultivation feed for greater amberjack broodstock include miscellaneous fish, Antarctic krill, eel meal, flour, multivitamins, Chinese herbal medicine, probiotic liquid, oysters and fish oil, and the Chinese herbal medicine includes hawthorn, Ligustrum lucidum, salvia miltiorrhiza, Cynomorium fortunei, Ligusticum chuanxiong and Astragalus membranaceus.

[0070] In some embodiments, the probiotic bacterial liquid comprises Bacillus subtilis and Lactobacillus.

[0071] In some embodiments, the raw materials for preparing the pre-spawning enhanced breeding feed for greater amberjack broodstock include 50-60 parts of miscellaneous fish, 14-16 parts of Antarctic krill, 14-16 parts of oysters, 9-11 parts of eel meal, 2-4 parts of fish oil, 1-5 parts of flour, 0.1-1 parts of complex vitamins, 0.1-1 parts of Chinese herbal medicine, and 1-2 parts of probiotic liquid.

[0072] In some embodiments, the raw materials for preparing the pre-natal enhanced breeding feed for greater amberjack broodstock include, by weight, 55 parts of miscellaneous fish, 14 parts of Antarctic krill, 14 parts of oysters, 10 parts of eel meal, 2 parts of fish oil, 4 parts of flour, 0.5 parts of multivitamins, 0.5 parts of Chinese herbal medicine, and 2 parts of probiotic liquid.

[0073] In some embodiments, the raw materials for preparing the pre-natal enhanced breeding feed for greater amberjack broodstock include, by weight, 52 parts of miscellaneous fish, 15 parts of Antarctic krill, 15 parts of oysters, 10 parts of eel meal, 4 parts of fish oil, 3 parts of flour, 0.5 parts of multivitamins, 0.5 parts of Chinese herbal medicine, and 2 parts of probiotic liquid.

[0074] In some embodiments, the mass ratio of Crataegus pinnatifida, Fructus Ligustri Lucidi, Salvia miltiorrhiza, Rhizoma Cyrthospermi, Rhizoma Chuanxiong and Radix Astragali in the Chinese herbal medicine is 1:1:1:1:1:1:1.

[0075] In some embodiments, the probiotic bacterial solution comprises a Bacillus subtilis bacterial solution and a Lactobacillus bacterial solution, the volume ratio of the two bacterial solutions is 1:1, and the concentration of the two bacterial solutions is 1×10 6 -3×10 6 cfu / ml, preferably about 2×10 6 cfu / ml.

[0076] In some embodiments, the pre-spawning fortified cultivation feed for greater amberjack broodstock is a soft pellet feed.

[0077] In some embodiments, the soft pellet feed has a particle size of 1.4-1.6 cm and a length of 4-6 cm.

[0078] In some embodiments, the trash fish comprises mackerel and triggerfish.

[0079] In some embodiments, the intensive pre-spawning cultivation period for broodstock refers to the period from 60 days before spawning of broodstock to the implementation of induced spawning.

[0080] Through intensive pre-spawning cultivation, the greater amberjack broodstock are placed in an environment that meets their breeding needs in advance, thereby reducing their stress ability during induced spawning and improving the stability of broodstock reproduction.

[0081] In some embodiments, 3-5 days after the broodstock are released, the broodstock are started to be cultured and fed with pre-spawning enhanced culture feed, with an average daily feeding amount of 2-3% of the broodstock body weight, gradually increased to 6%-7% of the broodstock body weight depending on the food competition situation, and fed 1-2 times a day.

[0082] For broodstock that lay eggs normally and produce more than 50% of their eggs, there is no need for secondary ovulation stimulation. It is only necessary to ensure that the eggs of the female broodstock are basically ovulated.

[0083] In some embodiments, the secondary ovulation induction comprises gently pressing the abdomen of the female broodstock to squeeze out the eggs, or injecting oxytocin into the female broodstock. If the genital opening is blocked, the genital opening should be pierced to prevent water absorption and swelling.

[0084] In some embodiments, the oxytocin comprises a gonadotropin-releasing hormone agonist, dioconazole, and an extract of the pituitary gland of Epinephelus coioides.

[0085] In some embodiments, the coioidal grouper is a 3-4 year old coioidal grouper, preferably a 3 year old coioidal grouper.

[0086] In some embodiments, the injection dose of oxytocin in the second stimulation is 0.5 mL / kg fish body weight.

[0087] In some embodiments, the oxytocin injected in the secondary ovulation induction contains 1 mg / mL gonadotropin-releasing hormone agonist, 50 mg / mL dioconazole and 1 mg / mL coiotrophobia grouper pituitary extract.

[0088] In some embodiments, the process of secondary ovulation promotion also includes the step of inserting a male parent that is healthy, disease-free, and has not been used. Inserting a male parent that is healthy, disease-free, and has not been used can help secondary ovulation promotion.

[0089] In some embodiments, the raw materials for preparing the greater amberjack broodstock postpartum care feed include miscellaneous fish, Antarctic krill, eel meal, flour, complex vitamins, Chinese herbal medicine, probiotic bacterial liquid, sandworms, squid paste and complex amino acids, and the Chinese herbal medicine includes hawthorn, cyrtomium, astragalus, honeysuckle, licorice and codonopsis.

[0090] In some embodiments, the probiotic bacterial liquid comprises Bacillus subtilis and Lactobacillus.

[0091] In some embodiments, the raw materials for preparing the greater amberjack broodstock postpartum care feed include 55-65 parts of miscellaneous fish, 14-16 parts of Antarctic krill, 9-11 parts of sandworms, 9-11 parts of eel powder, 2-4 parts of squid paste, 1-5 parts of flour, 0.1-1 parts of complex vitamins, 0.1-1 parts of complex amino acids, 0.1-1 parts of Chinese herbal medicine, and 1-3 parts of probiotic bacterial liquid, and the bacterial liquid concentration is 1×10 6 -3×106 cfu / ml.

[0092] In some embodiments, the raw materials for preparing the feed include, by mass, 60 parts of miscellaneous fish, 14 parts of Antarctic krill, 9 parts of sandworms, 10 parts of eel meal, 2 parts of squid paste, 3 parts of flour, 0.5 parts of complex vitamins, 0.5 parts of complex amino acids, 1 part of Chinese herbal medicine, and 2 parts of probiotic liquid.

[0093] In some embodiments, the raw materials for preparing the feed include, by mass, 57 parts of miscellaneous fish, 15 parts of Antarctic krill, 10 parts of sandworms, 10 parts of eel meal, 3 parts of squid paste, 3 parts of flour, 0.5 parts of complex vitamins, 0.5 parts of complex amino acids, 1 part of Chinese herbal medicine, and 2 parts of probiotic liquid.

[0094] In some embodiments, the mass ratio of hawthorn, cyrtomium, astragalus, honeysuckle, licorice, and codonopsis in the Chinese herbal medicine is 1:1:1:1:1:1:1.

[0095] In some embodiments, the probiotic bacterial solution comprises a Bacillus subtilis bacterial solution and a Lactobacillus bacterial solution, the volume ratio of the two bacterial solutions is 1:1, and the concentration of the two bacterial solutions is 1×10 6 -3×10 6 cfu / ml, preferably about 2×10 6 cfu / ml.

[0096] In some embodiments, the greater amberjack broodstock postpartum care feed is a soft pellet feed.

[0097] In some embodiments, the soft pellet feed has a particle size of 1.4-1.6 cm and a length of 4-6 cm.

[0098] In some embodiments, the trash fish includes mackerel and triggerfish, such as blue round trevally and devil triggerfish.

[0099] In some embodiments, the greater amberjack broodstock are fed with postpartum care feed 3-5 days after birth, and the daily feeding amount of the greater amberjack broodstock postpartum care feed is 5-7% of the broodstock body weight. The feeding amount is appropriately increased or decreased according to the broodstock's food snatching situation, and the broodstock are fed 1-2 times a day without any food snatching.

[0100] In some embodiments, the greater amberjack broodstock are wild domesticated, healthy, and energetic greater amberjack over 3 years old from Guangdong, Fujian, etc.

[0101] In some embodiments, the greater amberjack broodstock is a greater amberjack with a symmetrical body shape, a smooth and uninjured body surface, a strong physique, good feeding, strong vitality, no disease or deformity, a body length of ≥70 cm, and a body weight of ≥7 kg.

[0102] In some embodiments, the ratio of male broodstock to female broodstock in the greater amberjack broodstock is 1:1.

[0103] In some embodiments, greater amberjack broodstock are cultured in cages in an open sea area with smooth tides, no pollution, flat seabed, mainly muddy and sandy bottom, and far away from the coastline.

[0104] In some embodiments, greater amberjack broodstock are cultured in cages in a sea area with a water flow of ≤1.5m / s, a water temperature of ≥20°C, a salinity of 30‰-40‰, a pH of 8±0.5, a dissolved oxygen content of ≥5mg / L, a minimum tide depth of ≥12m, smooth water flow, small wind and waves, and water quality that meets GB11607.

[0105] In some embodiments, the net of the net cage is made of polyethylene or nylon material. When a knotted net is woven from polyethylene material, it is advisable to place the broodstock after a small amount of attachments are attached to the sea, which can reduce friction on the fish body. The net cage needs to be selected according to the size of the greater amberjack broodstock, such as a net cage with a specification of 10*10*5. The mesh size is based on the fish being unable to escape, so it should be determined according to the size of the fish body, such as a mesh size of 3-6 cm.

[0106] In some embodiments, the stocking density of greater amberjack in the cage is ≤ 6 fish / 10m 3 .

[0107] In some embodiments, the selected greater amberjack broodstock need to be sterilized and dewormed before pre-spawning intensive cultivation.

[0108] In some embodiments, sterilization and deworming are performed by soaking. Specifically, the broodstock are soaked in seawater added with hydrogen peroxide solution for disinfection and deworming. When the concentration of hydrogen peroxide solution is 30% (mass concentration), 200-300 ml of hydrogen peroxide solution is added per cubic meter of seawater. If hydrogen peroxide solution of other concentrations is used, the added volume of hydrogen peroxide solution can be calculated according to the addition ratio. After the broodstock are soaked and disinfected for 5-10 minutes, they are placed in the cage for cultivation. After that, the broodstock are soaked and disinfected once every 20 days.

[0109] In some embodiments, the postpartum care also includes the step of performing anti-inflammatory and disinfection treatment on the parent fish. Specifically, the parent fish are examined, and glucose and doxycycline hydrochloride are injected into the fish without body surface damage for internal anti-inflammatory treatment (wherein the glucose injection dose is 0.5 ml per kilogram of body weight, and the doxycycline hydrochloride injection dose is 1 mg per kilogram of body weight), and glucose, penicillin and compound amino acids are injected into the fish with serious injuries (wherein the glucose injection dose is 0.5 ml per kilogram of body weight, the penicillin injection dose is 100,000 units per kilogram of body weight, and the compound amino acid injection dose is 20 mg per kilogram of body weight), and then all the parent fish are placed in seawater added with hydrogen peroxide solution and immersed for 5-10 minutes for disinfection, and veterinary streptomycin or penicillin is placed in the solution to achieve better anti-inflammatory and wound healing effects. When the concentration of hydrogen peroxide solution is 30% (mass concentration), 200-300 ml of hydrogen peroxide solution is added per cubic meter of seawater. If hydrogen peroxide solution of other concentrations is used, the added volume of hydrogen peroxide solution can be calculated according to the addition ratio.

[0110] In some embodiments, during the postpartum care process, the health status of the broodstock can also be checked regularly, diseases can be discovered and treated in a timely manner, sampling can be performed with water as much as possible to reduce unnecessary damage to the fish body, and the broodstock can be checked regularly for any abnormal symptoms to achieve early detection, early diagnosis, and early treatment to prevent the spread of the disease, and dead fish can be removed in a timely manner for harmless disposal.

[0111] The beneficial effects of the present invention are as follows: the present application provides a greater amberjack broodstock feed containing Chinese herbal medicines, and by matching different raw materials, the greater amberjack broodstock feed can better provide nutrients and active substances for greater amberjack broodstock. Before giving birth, it can enhance the physical fitness and immune ability of broodstock, promote the development of broodstock, accelerate sexual maturity, increase the rate of induction of labor, egg production, fertilization rate, and hatching rate; after giving birth, it can promote the healing of broodstock and restore physical fitness, thereby ensuring the survival rate and quality of broodstock after giving birth.

[0112] The present invention also provides a breeding method using the greater amberjack broodstock feed, which reduces the stress ability of the broodstock during induced spawning through intensive pre-natal cultivation, improves the stability of broodstock reproduction, and enhances the physical fitness and immune ability of the broodstock through feeding the greater amberjack broodstock feed, promotes the development of the broodstock, accelerates sexual maturity, and improves the induced spawning rate, egg laying amount, fertilization rate, and hatching rate. DETAILED DESCRIPTION

[0113] The present invention is further described in detail below by specific examples. Unless otherwise specified, the raw materials, reagents or devices used in the examples and comparative examples can be obtained from conventional commercial sources or can be obtained by prior art methods. Unless otherwise specified, the experiments or test methods are conventional methods in the art.

[0114] The composite vitamins used in the embodiment of the present invention are instant electrolytic multivitamins produced by Jiangsu Aotewo Aquatic Products Technology Co., Ltd., with the product number A30KY; the composite amino acids used in the embodiment of the present invention are soybean composite amino acid powder (80%) produced by Sichuan Mianzhu Ruiyang Biotechnology Co., Ltd.

[0115] Example 1

[0116] 30 3-year-old greater amberjacks with well-developed gonads, healthy and vigorous, body length ≥70cm, body weight ≥7kg, sex ratio 1:1 were selected as broodstock and placed in a 10*10*5 net cage made of nylon netting for breeding. The breeding period was from about 60 days before spawning of broodstock until the implementation of induced spawning. The cages were placed in the sea area of ​​Qixia Village, Dongshan County, Fujian Province, where the water flow was ≤1.2m / s, the water temperature was ≥21℃, the salinity was ≥30‰, the pH was 8±0.5, the dissolved oxygen content was ≥6mg / L, the lowest tide depth was ≥12m, and the offshore distance was about 4km. After the selection is completed, the greater amberjack broodstock are first placed in a water bag for immersion and disinfection. The water bag contains seawater with hydrogen peroxide solution added. 200 ml of hydrogen peroxide solution is added per cubic meter of seawater, and the concentration of hydrogen peroxide solution is 30% (mass concentration). The broodstock are immersed and disinfected for 10 minutes and then placed in a net cage for cultivation. Thereafter, the broodstock are immersed and disinfected once every 20 days.

[0117] Three days after the broodstock were released, the cultivation and feeding began. The initial feeding amount was about 3% of the broodstock's body weight. At this time, the broodstock stopped competing for food. On the fifth day, when the feeding amount increased to about 6% of the broodstock's body weight, the competition for food stopped. From then on until the breeding period, the feeding amount was 6-7% of the broodstock's body weight. The feed included the following components by mass percentage: 55 parts of miscellaneous fish (including blue round trevally and devil triggerfish), 14 parts of Antarctic krill, 14 parts of oysters, 10 parts of eel meal, 2 parts of fish oil, 4 parts of flour, 0.5 parts of multivitamins, and 0.5 parts of Chinese herbal medicine (crataegus, Ligustrum lucidum, Salvia miltiorrhiza, Cynomorium fortunei, Ligusticum chuanxiong, and Astragalus membranaceus); first, the various components of the bait were crushed separately, and the crushed miscellaneous fish, Antarctic krill, and oysters were mixed. Then, the mixture was placed in a fermentation tank, and 2 parts of probiotic bacterial solution (including Bacillus subtilis bacterial solution and Lactobacillus bacterial solution, the volume ratio of the two bacterial solutions was 1:1, and the concentration of the two bacterial solutions was about 2×10 6 cfu / ml) into a fermentation tank and stirred evenly with the mixture, and the temperature of the fermentation tank was adjusted at 35-40°C for fermentation for 60 hours, after which the fermented mixture was evenly mixed with eel meal, fish oil, flour, multivitamins and Chinese herbal medicine powder, and extruded into soft granular feed with a particle size of 1.4-1.6 cm and a length of 4-6 cm by a granulator.

[0118] Artificial induction of labor: After the broodstock have finished spawning, check the ovulation status of the female broodstock, whether the abdomen is swollen, whether the outline of the ovaries is obvious, and whether a small amount of eggs flow out by gently squeezing the abdominal cloaca. For female broodstock with a high egg-laying amount, no secondary ovulation induction will be performed after it is determined that the eggs of the female broodstock are basically discharged. For broodstock that do not lay eggs or whose egg-laying amount does not exceed 50%, the female broodstock will be fished out and the eggs will be squeezed out by gently pressing the abdomen. For female broodstock that cannot be squeezed out, oxytocin will continue to be injected. The concentration of gonadotropin-releasing hormone agonist (GnRHa) in the oxytocin is 1 mg / ml, the concentration of diometron (DOM) is 50 mg / ml, and the concentration of pituitary extract of coiospermum coiospermum is 1 mg / ml. The volume ratio of the three is GnRHa:DOM:3-year-old coiospermum coiospermum pituitary extract=3:6:1, and the injection dose is 0.5mL / kg fish body weight. Secondary ovulation induction is performed to basically discharge all the eggs of the female parent. During the secondary ovulation induction process, a male parent that is not injured or diseased and has not been used before is placed to help discharge all the eggs.

[0119] The induced broodstock were examined, and the fish with no surface injuries were injected with glucose + doxycycline hydrochloride for internal anti-inflammatory treatment, with 0.5 ml of glucose per kilogram of body weight and 1 mg of doxycycline hydrochloride per kilogram of body weight. The seriously injured fish were injected with a mixture of glucose, penicillin and complex amino acids, with 0.5 ml of glucose per kilogram of body weight, 100,000 units of penicillin per kilogram of body weight and 20 mg of complex amino acids per kilogram of body weight. Then all the induced broodstock were placed in seawater added with hydrogen peroxide solution and immersed for 10 minutes for disinfection, with 200 ml of hydrogen peroxide solution added per cubic meter of seawater (the concentration of hydrogen peroxide in the hydrogen peroxide solution is 30%), and 5 million units of veterinary streptomycin or penicillin per liter of seawater were added to the solution to achieve better anti-inflammatory and wound healing effects.

[0120] After 3 days of temporary rearing (no feeding during the temporary rearing period), a small amount of feed was started, and the feed was fed in small amounts and multiple times a day. The feed included the following components by mass percentage: 60 parts of miscellaneous fish (including blue round scad and devil triggerfish), 14 parts of Antarctic krill, 9 parts of sandworms, 10 parts of eel powder, 2 parts of squid paste, 3 parts of flour, 0.5 parts of multivitamins, 0.5 parts of complex amino acids, and 1 part of Chinese herbal medicine (crataegus, cyperus, astragalus, honeysuckle, licorice, and codonopsis). First, the components of the bait were crushed separately, and the crushed miscellaneous fish, Antarctic krill, and sandworms were mixed. Then, the mixture was placed in a fermentation tank, and 2 parts of probiotic bacterial liquid (including Bacillus subtilis bacterial liquid and Lactobacillus bacterial liquid, the volume ratio of the two bacterial liquids was 1:1, and the concentration of the two bacterial liquids was about 2×10 6cfu / ml) into a fermentation tank and stirred evenly with the mixture, and the temperature of the fermentation tank was adjusted at 35-40°C for fermentation for 60 hours, and then the fermented mixture was evenly mixed with eel powder, squid paste, flour, complex vitamins, complex amino acids and Chinese herbal medicine powder, and extruded into soft granular feed with a particle size of 1.4-1.6 cm and a length of 4-6 cm by a granulator to enhance immunity and promote postpartum recovery. The daily feeding amount was gradually transitioned to 5-7% of the body weight of the broodstock according to the food grabbing situation of the broodstock, and the feeding was continued for 30 days.

[0121] During the postpartum care period of greater amberjack broodstock, a 30% hydrogen peroxide solution is selected regularly (every 15 days), 200 ml of hydrogen peroxide solution is added to each cubic meter of seawater, and the broodstock are immersed and disinfected for 10 minutes to prevent the occurrence of bacterial diseases. The broodstock are regularly checked for any abnormal symptoms, so as to achieve early detection, early diagnosis, and early treatment to prevent the spread of the disease, and the dead fish are promptly removed for harmless treatment. In this embodiment, one dead fish appears.

[0122] A total of 30 broodstock were selected for this experiment. No broodstock were lost during the pre-natal care period. There were 15 females, 7 of which were successfully induced to spawn, with an induced spawning rate of 46.7%. There were about 2.51 million fertilized eggs, a total of about 5.18 million eggs, a fertilization rate of 48.6%, and about 1.87 million fry were hatched, with a hatching rate of 74.2%. After completing the reproduction and passing the post-natal care for 30 days, a total of 29 broodstock survived, with a survival rate of 96.7%.

[0123] Example 2

[0124] 30 3-year-old greater amberjacks with well-developed gonads, healthy and vigorous, body length ≥70cm, body weight ≥7kg, sex ratio 1:1 were selected as broodstock and placed in a 10*10*5 net cage made of nylon netting for breeding. The breeding period was from about 60 days before spawning of broodstock until the implementation of induced spawning. The cages were placed in the sea area of ​​Qixia Village, Dongshan County, Fujian Province, where the water flow was ≤1.2m / s, the water temperature was ≥21℃, the salinity was ≥30‰, the pH was 8±0.5, the dissolved oxygen content was ≥6mg / L, the lowest tide depth was ≥12m, and the offshore distance was about 4km. After the selection is completed, the greater amberjack broodstock are first placed in a water bag for soaking and disinfection. The water bag contains seawater with hydrogen peroxide solution added. 300 ml of hydrogen peroxide solution is added per cubic meter of seawater, and the concentration of hydrogen peroxide solution is 30% (mass concentration). The broodstock are soaked and disinfected for 10 minutes and then placed in a net cage for cultivation. Thereafter, the broodstock are soaked and disinfected once every 20 days.

[0125] Three days after the broodstock were released, the cultivation and feeding began. The initial feeding amount was about 3% of the broodstock's body weight. At this time, the broodstock stopped competing for food. On the fifth day, when the feeding amount increased to about 6% of the broodstock's body weight, the competition for food stopped. From then on until the breeding period, the feeding amount was 6-7% of the broodstock's body weight. The feed included the following components by mass percentage: 52 parts of miscellaneous fish (including blue round trevally and devil triggerfish), 15 parts of Antarctic krill, 15 parts of oysters, 10 parts of eel meal, 4 parts of fish oil, 3 parts of flour, 0.5 parts of multivitamins, and 0.5 parts of Chinese herbal medicine (crataegus, Ligustrum lucidum, Salvia miltiorrhiza, Cynomorium fortunei, Ligusticum chuanxiong, and Astragalus membranaceus); first, the various components of the bait were crushed separately, and the crushed miscellaneous fish, Antarctic krill, and oysters were mixed. Then, the mixture was placed in a fermentation tank, and a certain amount of probiotics solution (including Bacillus subtilis solution and Lactobacillus solution, the volume ratio of the two solutions was 1:1, and the concentration of the two solutions was about 2×10 6 cfu / ml) into a fermentation tank and stirred evenly with the mixture, and the temperature of the fermentation tank was adjusted at 35-40°C for fermentation for 60 hours, after which the fermented mixture was evenly mixed with eel meal, fish oil, flour, multivitamins and Chinese herbal medicine powder, and extruded into soft granular feed with a particle size of 1.4-1.6 cm and a length of 4-6 cm by a granulator.

[0126] Artificial induction of labor: After the broodstock have finished spawning, check the ovulation status of the female broodstock, whether the abdomen is swollen, whether the outline of the ovaries is obvious, and whether a small amount of eggs flow out by gently squeezing the abdominal cloaca. For female broodstock with a high egg-laying amount, no secondary ovulation induction will be performed after it is determined that the eggs of the female broodstock are basically discharged. For broodstock that do not lay eggs or whose egg-laying amount does not exceed 50%, the female broodstock will be fished out and the eggs will be squeezed out by gently pressing the abdomen. For female broodstock that cannot be squeezed out, oxytocin will continue to be injected. The concentration of gonadotropin-releasing hormone agonist (GnRHa) in the oxytocin is 1 mg / ml, the concentration of diometron (DOM) is 50 mg / ml, and the concentration of pituitary extract of coiospermum coiospermum is 1 mg / ml. The volume ratio of the three is GnRHa:DOM:3-year-old coiospermum coiospermum pituitary extract=3:6:1, and the injection dose is 0.5mL / kg fish body weight. Secondary ovulation induction is performed to basically discharge all the eggs of the female parent. During the secondary ovulation induction process, a male parent that is not injured or diseased and has not been used before is placed to help discharge all the eggs.

[0127] The induced broodstock were examined, and the fish with no surface injuries were injected with glucose + doxycycline hydrochloride for internal anti-inflammatory treatment, with 0.5 ml of glucose per kilogram of body weight and 1 mg of doxycycline hydrochloride per kilogram of body weight. The seriously injured fish were injected with a mixture of glucose, penicillin and complex amino acids, with 0.5 ml of glucose per kilogram of body weight, 100,000 units of penicillin per kilogram of body weight and 20 mg of complex amino acids per kilogram of body weight. Afterwards, all the induced broodstock were immersed in seawater added with hydrogen peroxide solution for 10 minutes for disinfection, 200 ml of hydrogen peroxide solution was added per cubic meter of seawater (the concentration of hydrogen peroxide in the hydrogen peroxide solution was 30%), and 5 million units of veterinary streptomycin or penicillin per liter of seawater were added to the solution to achieve better anti-inflammatory and wound healing effects.

[0128] After 3 days of temporary rearing, a small amount of feed was fed (no feeding during the temporary rearing period), and the feed was fed in small amounts and multiple times a day. The feed included the following components by mass percentage: 57 parts of miscellaneous fish (including blue round trevally and devil triggerfish), 15 parts of Antarctic krill, 10 parts of sandworms, 10 parts of eel powder, 3 parts of squid paste, 3 parts of flour, 0.5 parts of multivitamins, 0.5 parts of complex amino acids, and 1 part of Chinese herbal medicine (crataegus, cyperus, astragalus, honeysuckle, licorice, and codonopsis). First, the components of the bait were crushed separately, and the crushed miscellaneous fish, Antarctic krill, and sandworms were mixed. Then, the mixture was placed in a fermentation tank, and 2 parts of probiotic bacterial liquid (including Bacillus subtilis bacterial liquid and Lactobacillus bacterial liquid, the volume ratio of the two bacterial liquids was 1:1, and the concentration of the two bacterial liquids was about 2×10 6 cfu / ml) into a fermentation tank and stirred evenly with the mixture, and the temperature of the fermentation tank was adjusted at 35-40°C for fermentation for 60 hours, and then the fermented mixture was evenly mixed with eel powder, squid paste, flour, complex vitamins, complex amino acids and Chinese herbal medicine powder, and extruded into soft granular feed with a particle size of 1.4-1.6 cm and a length of 4-6 cm by a granulator to enhance immunity and promote postpartum recovery. The daily feeding amount was gradually transitioned to 5-7% of the body weight of the broodstock according to the food grabbing situation of the broodstock, and the feeding was continued for 30 days.

[0129] During the postpartum care period of the greater amberjack broodstock, a 30% hydrogen peroxide solution is selected regularly (every 15 days), 300 ml of hydrogen peroxide solution is added to each cubic meter of seawater, and the broodstock are immersed and disinfected for 10 minutes to prevent the occurrence of bacterial diseases. The broodstock are regularly checked for any abnormal symptoms, so as to achieve early detection, early diagnosis, and early treatment to prevent the spread of the disease, and the dead fish are promptly removed for harmless treatment. No dead fish appeared in this embodiment.

[0130] A total of 30 broodstock were selected for this experiment. No broodstock were lost during the pre-natal care period. There were 15 females, 8 of which were successfully induced to spawn, with an induced spawning rate of 53.3%. There were about 3.05 million fertilized eggs, a total of about 5.83 million eggs, a fertilization rate of 52.4%, and about 2.39 million fry were hatched, with a hatching rate of 74.2%. After completing the reproduction and passing the post-natal care for 30 days, a total of 30 broodstock survived, with a survival rate of 100.0%.

[0131] Comparative Example 1

[0132] 30 3-year-old greater amberjacks with well-developed gonads, healthy and vigorous, body length ≥70cm, body weight ≥7kg, sex ratio 1:1 were selected as broodstock and placed in a 10*10*5 net cage made of nylon netting for breeding. The breeding period was from about 60 days before spawning of broodstock until the implementation of induced spawning. The cages were placed in the sea area of ​​Qixia Village, Dongshan County, Fujian Province, where the water flow was ≤1.2m / s, the water temperature was ≥21℃, the salinity was ≥30‰, the pH was 8±0.5, the dissolved oxygen content was ≥6mg / L, the lowest tide depth was ≥12m, and the offshore distance was about 4km. After the selection is completed, the greater amberjack broodstock are first placed in a water bag for soaking and disinfection. The water bag contains seawater with hydrogen peroxide solution added. 300 ml of hydrogen peroxide solution is added per cubic meter of seawater, and the concentration of hydrogen peroxide solution is 30% (mass concentration). The broodstock are soaked and disinfected for 10 minutes and then placed in a net cage for cultivation. Thereafter, the broodstock are soaked and disinfected once every 20 days.

[0133] Three days after the broodstock were released, they were fed and the initial feeding amount was about 3% of the broodstock's body weight. At this time, the broodstock stopped competing for food. On the fifth day, when the feeding amount was increased to about 6% of the broodstock's body weight, the competition for food stopped. From then on until the breeding period, the feeding amount was 6-7% of the broodstock's body weight. The feed was fresh ice fish.

[0134] Artificial induction of spawning: after the broodstock have finished spawning, all broodstock are placed in seawater with hydrogen peroxide solution for 10 minutes for disinfection. 200 ml of hydrogen peroxide solution is added per cubic meter of seawater (the concentration of hydrogen peroxide in the hydrogen peroxide solution is 30%), and 5 million units of veterinary streptomycin or penicillin per liter of seawater are added to the solution to achieve better anti-inflammatory and wound healing effects. After 3 days of temporary feeding, a small amount of feed is started, and the feed is fed in small amounts and multiple times a day. The feed is fresh ice fish, and the daily feeding amount gradually transitions to 5-7% of the body weight of the broodstock according to the broodstock's food competition, and continues to feed for 30 days.

[0135] A total of 30 broodstock were selected for this experiment. No broodstock were lost during the pre-natal care period. There were 15 females, 5 of which were successfully induced to spawn, with an induced spawning rate of 33.3%. There were about 1.4 million fertilized eggs, a total of about 3.2 million eggs, a fertilization rate of 43.7%, and about 970,000 fry were hatched, with a hatching rate of 69.7%. After completing the reproduction and passing the post-natal care for 30 days, a total of 26 broodstock survived, with a survival rate of 86.7%.

[0136] Table 1 Statistical results of induced spawning rate, egg laying rate, fertilization rate, hatching rate and post-natal parent fish survival rate of Examples 1-2 and Comparative Example 1

[0137]

[0138] It can be seen from Table 1 that after soaking and disinfection and then placing the fish in the cage for cultivation, Comparative Example 1 did not undergo prenatal nutritional fortification, nor did it undergo secondary postnatal ovulation promotion or increase in nutritional supply. Therefore, compared with Examples 1-2, the induction rate, egg laying rate, fertilization rate, hatching rate and postnatal broodstock survival rate were significantly reduced.

[0139] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A greater amberjack broodstock feed, wherein the raw materials for preparing the feed include trash fish, Antarctic krill, eel meal, flour, multivitamins, Chinese herbal medicine and probiotic liquid.

2. The feed according to claim 1, characterized in that The probiotic bacterial liquid contains Bacillus subtilis and Lactobacillus; Preferably, the probiotic bacterial liquid contains Bacillus subtilis and Lactobacillus.

3. The feed according to claim 1, characterized in that The feed is soft pellet feed.

4. The feed according to any one of claims 1 to 3, characterized in that The feed preparation raw materials also include oysters and fish oil; the Chinese herbal medicines include hawthorn, Ligustrum lucidum, salvia miltiorrhiza, Cyperus rotundus, Ligusticum chuanxiong and Astragalus membranaceus; Preferably, the feed comprises, by weight, 50-60 parts of trash fish, 14-16 parts of Antarctic krill, 14-16 parts of oysters, 9-11 parts of eel meal, 2-4 parts of fish oil, 1-5 parts of flour, 0.1-1 parts of multivitamins, 0.1-1 parts of Chinese herbal medicine, and 1-3 parts of probiotics liquid, with a liquid concentration of 1×10 6 -3×10 6 cfu / ml; Preferably, the mass ratio of hawthorn, glossy privet fruit, salvia miltiorrhiza, cyrtomium, ligusticum chuanxiong and astragalus in the Chinese herbal medicine is 1:1:1:1:1:1:

1.

5. The feed according to any one of claims 1 to 3, wherein the feed preparation raw materials further include nereidia, squid paste and composite amino acids; the Chinese herbal medicines include hawthorn, cyperus, astragalus, honeysuckle, liquorice and codonopsis; Preferably, the feed comprises, by weight, 55-65 parts of trash fish, 14-16 parts of Antarctic krill, 9-11 parts of sandworms, 9-11 parts of eel powder, 2-4 parts of squid paste, 1-5 parts of flour, 0.1-1 parts of complex vitamins, 0.1-1 parts of complex amino acids, 0.1-1 parts of Chinese herbal medicine, and 1-3 parts of probiotics liquid, and the concentration of the liquid is 1×10 6 -3×10 6 cfu / ml; Preferably, the mass ratio of hawthorn, cyrtomium, astragalus, honeysuckle, liquorice and codonopsis in the Chinese herbal medicine is 1:1:1:1:1:1:

1.

6. A reagent combination for artificial breeding of greater amberjack, comprising oxytocin, an anti-inflammatory composition and the feed according to any one of claims 1 to 5; Preferably, the oxytocin comprises gonadotropin-releasing hormone agonist, dioconazole and pituitary extract of Epinephelus coioides; Preferably, the anti-inflammatory composition comprises: (a) dextrose and doxycycline hydrochloride; and / or (b) Glucose, penicillin and complex amino acids.

7. Use of the feed according to any one of claims 1 to 5 and the reagent according to claim 6 in combination in artificial breeding of greater amberjack.

8. A method for artificial breeding of greater amberjack, comprising the following steps: Pre-spawning intensive cultivation of broodstock: Use pre-spawning intensive cultivation feed for greater amberjack broodstock to cultivate broodstock; Secondary ovulation promotion: Secondary ovulation promotion is carried out on female broodstock that do not lay eggs or whose egg laying amount does not exceed 50%; Postpartum care: After the greater amberjack broodstock have completed reproduction, the broodstock are cultured using greater amberjack broodstock postpartum care feed; The raw materials for preparing the pre-natal enhanced cultivation feed for greater amberjack broodstock include miscellaneous fish, Antarctic krill, eel meal, flour, multivitamins, Chinese herbal medicine, probiotic liquid, oysters and fish oil, and the Chinese herbal medicine includes hawthorn, Ligustrum lucidum, salvia miltiorrhiza, Cynomorium fortunei, Ligusticum chuanxiong and Astragalus membranaceus.

9. The artificial breeding method according to claim 8, characterized in that: The secondary ovulation induction comprises lightly pressing the abdomen of the female broodstock to squeeze out the eggs, or injecting oxytocin into the female broodstock; preferably, the oxytocin comprises gonadotropin-releasing hormone agonist, dioconstrictor and pituitary extract of Epinephelus coioides.

10. The artificial breeding method according to claim 8, characterized in that: The raw materials for preparing the greater amberjack broodstock postpartum care feed include miscellaneous fish, Antarctic krill, sandworms, eel powder, squid paste, flour, complex vitamins, complex amino acids, Chinese herbal medicines and probiotic bacterial liquid; the Chinese herbal medicines include hawthorn, cyrtomium, astragalus, honeysuckle, licorice and codonopsis.

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