A method for pre-natal cultivation and post-natal care of cobia broodstock

Abstract

The application discloses a high-body cobia broodstock feed containing Chinese herbal medicines and a high-body cobia breeding method using the feed, and by matching different raw materials, the high-body cobia broodstock feed can better provide the high-body cobia broodstock with nutrient components and active substances. Before spawning, the high-body cobia broodstock feed can enhance the physical fitness and immunity of the broodstock, promote the development of the broodstock, accelerate sexual maturation, improve the spawning rate, the egg production, the fertilization rate and the hatching rate, and after spawning, the high-body cobia broodstock feed can promote the healing of the broodstock, restore the physical fitness, and guarantee the survival rate and quality of the broodstock after spawning.

Description

Technical Field

[0001] This invention relates to the field of aquaculture technology, and in particular to a method for pre-spawning cultivation and post-spawning care of high-body yellowtail broodstock. Background Technology

[0002] The amberjack (Seriola dumerili), belonging to the family Carangidae in the order Perciformes, is a large, warm-temperate fish that inhabits the upper and middle layers of the ocean. It is widely distributed in tropical and subtropical waters of all oceans and is one of the precious fish species in the South my country Sea. It grows relatively quickly, has tender flesh, and a delicious flavor. It is rich in protein, linolenic acid, linoleic acid, and other essential fatty acids, as well as highly unsaturated fatty acids (EPA and DHA), making it an excellent choice for sashimi. Commercial aquaculture has been implemented in many regions. Amberjacks reach sexual maturity at 3-4 years of age. Although artificial breeding techniques for amberjacks have made breakthroughs, the spawning rate, egg production, fertilization rate, and hatching rate are still relatively low. The quality of the broodstock is crucial in artificial fish breeding; only good broodstock can ensure high-quality fry. Therefore, the cultivation of broodstock is of paramount importance.

[0003] Improving the spawning rate, egg production, fertilization rate, and hatching rate of high-body amberjack, as well as reducing postpartum mortality of broodstock, is one of the key technologies for large-scale artificial breeding of high-body amberjack. Therefore, proper pre-spawning rearing and post-spawning care of high-body amberjack broodstock are crucial for ensuring increased aquaculture production and are of great significance for the sustainable development of the high-body amberjack industry. Summary of the Invention

[0004] The first aspect of the present invention provides a high-body amberjack broodstock feed, wherein the raw materials for preparing the feed include miscellaneous fish, Antarctic krill, eel meal, flour, compound vitamins, traditional Chinese medicine and probiotic liquid.

[0005] Traditional Chinese medicine (TCM) herbs are natural and green feed additives, rich in bioactive substances and nutrients. They can improve animal digestion, growth performance, and non-specific immune function. Furthermore, TCM herbs, with their unique antimicrobial and antiparasitic functions and mechanisms of action, are less likely to induce drug resistance. This invention adds TCM herbs to the feed of high-body amberjack broodstock, enabling it to better provide nutrients and bioactive substances to the amberjack broodstock.

[0006] In some embodiments, the feed preparation ingredients also include oysters and fish oil, and the traditional Chinese medicines include hawthorn, privet fruit, salvia miltiorrhiza, dryopteris crassirhizoma, chuanxiong rhizome, and astragalus root.

[0007] By further adding oysters and fish oil to the raw materials for feed preparation, and adding traditional Chinese medicines including hawthorn, privet fruit, salvia miltiorrhiza, dryopteris crassirhizoma, chuanxiong rhizome and astragalus, the prepared feed is more suitable for pre-spawning intensive breeding of high-body yellowtail broodstock, enhancing the physical strength and immunity of broodstock, promoting the development of broodstock, accelerating sexual maturity, and improving spawning rate, egg production, fertilization rate and hatching rate.

[0008] In some embodiments, the probiotic solution contains Bacillus subtilis and Lactobacillus.

[0009] In some embodiments, the feed preparation ingredients, by weight, comprise 50-60 parts miscellaneous fish, 14-16 parts Antarctic krill, 14-16 parts oysters, 9-11 parts eel meal, 2-4 parts fish oil, 1-5 parts flour, 0.1-1 part compound vitamins, 0.1-1 part traditional Chinese medicine, and 1-3 parts probiotic liquid with a concentration of 1×10⁻⁶. 6 -3×10 6 cfu / ml.

[0010] In some embodiments, the feed preparation ingredients, by weight, include 55 parts of miscellaneous fish, 14 parts of Antarctic krill, 14 parts of oysters, 10 parts of eel powder, 2 parts of fish oil, 4 parts of flour, 0.5 parts of compound vitamins, 0.5 parts of traditional Chinese medicine, and 2 parts of probiotic liquid.

[0011] In some embodiments, the feed preparation ingredients, by weight, include 52 parts of miscellaneous fish, 15 parts of Antarctic krill, 15 parts of oysters, 10 parts of eel powder, 4 parts of fish oil, 3 parts of flour, 0.5 parts of compound vitamins, 0.5 parts of traditional Chinese medicine, and 2 parts of probiotic liquid.

[0012] In some embodiments, the mass ratio of hawthorn, privet fruit, salvia miltiorrhiza, dryopteris crassirhizoma, chuanxiong rhizome, and astragalus root in the traditional Chinese medicine is 1:1:1:1:1:1.

[0013] In some embodiments, the probiotic solution comprises Bacillus subtilis and Lactobacillus, with a volume ratio of 1:1 and a concentration of 1×10⁻⁶ for both solutions. 6 -3×10 6 cfu / ml, preferably about 2×10 6 cfu / ml.

[0014] In some embodiments, the feed is a soft pellet feed.

[0015] Soft pellet feed is a green and environmentally friendly aquatic feed. During the production process, it can not only produce a large number of beneficial bacteria and metabolites, which can improve the nutritional composition and palatability of the feed, promote the digestion and absorption of nutrients by high-body amberjack, and improve feed conversion efficiency, but also inhibit the reproduction of harmful microorganisms, thereby improving the production performance and disease resistance of high-body amberjack.

[0016] In some embodiments, the soft pellet feed has a particle size of 1.4-1.6 cm and a length of 4-6 cm.

[0017] In some embodiments, the miscellaneous fish includes mackerel and triggerfish.

[0018] In some embodiments, the method for preparing the feed includes:

[0019] Crush the mixed fish, Antarctic krill, and oysters separately and then mix them together;

[0020] Place the mixture in a fermenter, add probiotic liquid to the fermenter and stir it evenly, and adjust the temperature of the fermenter to 35-40℃ for 48-72 hours.

[0021] Mix the fermented mixture evenly with eel powder, fish oil, flour, multivitamins, and Chinese herbal powder.

[0022] The feed is produced by extruding the pellets into soft pellets with a diameter of 1.4-1.6 cm and a length of 4-6 cm using a pellet mill.

[0023] In some embodiments, the raw materials for preparing the feed also include sandworm, squid paste and compound amino acids, and the traditional Chinese medicine includes hawthorn, Dryopteris crassirhizoma, Astragalus membranaceus, honeysuckle, licorice and Codonopsis pilosula.

[0024] Further addition of sandworm, squid paste, and compound amino acids to the raw materials for feed preparation, along with the addition of traditional Chinese medicinal herbs including hawthorn, Dryopteris crassirhizoma, Astragalus membranaceus, honeysuckle, licorice, and Codonopsis pilosula, makes the prepared feed more suitable for increasing the nutritional supply to broodstock after spawning, promoting the recovery of broodstock's physical condition, and ensuring the survival rate and quality of broodstock after spawning.

[0025] In some embodiments, the probiotic solution contains Bacillus subtilis and Lactobacillus.

[0026] In some embodiments, the feed preparation ingredients, by weight, comprise 55-65 parts miscellaneous fish, 14-16 parts Antarctic krill, 9-11 parts sandworms, 9-11 parts eel meal, 2-4 parts squid paste, 1-5 parts flour, 0.1-1 part compound vitamins, 0.1-1 part compound amino acids, 0.1-1 part traditional Chinese medicine, and 1-3 parts probiotic liquid with a concentration of 1×10⁻⁶. 6 -3×10 6 cfu / ml.

[0027] In some embodiments, the feed preparation ingredients, by weight, include 60 parts of miscellaneous fish, 14 parts of Antarctic krill, 9 parts of sandworms, 10 parts of eel powder, 2 parts of squid paste, 3 parts of flour, 0.5 parts of compound vitamins, 0.5 parts of compound amino acids, 1 part of traditional Chinese medicine, and 2 parts of probiotic liquid.

[0028] In some embodiments, the feed preparation ingredients, by weight, include 57 parts of miscellaneous fish, 15 parts of Antarctic krill, 10 parts of sandworms, 10 parts of eel powder, 3 parts of squid paste, 3 parts of flour, 0.5 parts of compound vitamins, 0.5 parts of compound amino acids, 1 part of traditional Chinese medicine, and 2 parts of probiotic liquid.

[0029] In some embodiments, the mass ratio of hawthorn, Dryopteris crassirhizoma, Astragalus membranaceus, honeysuckle, licorice, and Codonopsis pilosula in the traditional Chinese medicine is 1:1:1:1:1:1.

[0030] In some embodiments, the probiotic solution comprises Bacillus subtilis and Lactobacillus, with a volume ratio of 1:1 and a concentration of 1×10⁻⁶ for both solutions. 6 -3×10 6 cfu / ml, preferably about 2×10 6 cfu / ml.

[0031] In some embodiments, the feed is a soft pellet feed.

[0032] In some embodiments, the soft pellet feed has a particle size of 1.4-1.6 cm and a length of 4-6 cm.

[0033] In some embodiments, the miscellaneous fish includes mackerel and triggerfish.

[0034] In some embodiments, the method for preparing the feed includes:

[0035] Crush the miscellaneous fish, Antarctic krill, and sandworms separately and then mix them together;

[0036] Place the mixture in a fermenter, add probiotic liquid to the fermenter and stir it evenly, and adjust the temperature of the fermenter to 35-40℃ for 48-72 hours.

[0037] The fermented mixture is mixed evenly with eel powder, squid paste, flour, compound vitamins, compound amino acids and traditional Chinese medicine powder, and then extruded into soft pellet feed with a particle size of 1.4-1.6cm and a length of 4-6cm through a pellet mill.

[0038] A second aspect of this invention provides a reagent combination for the artificial breeding of high-body amberjack, comprising oxytocin, an anti-inflammatory composition, and a high-body amberjack broodstock feed; the high-body amberjack broodstock feed includes a pre-spawning intensive rearing feed and a post-spawning care feed, the raw materials for preparing the high-body amberjack broodstock feed comprising miscellaneous fish, Antarctic krill, eel meal, flour, compound vitamins, traditional Chinese medicine, and probiotic liquid. The oxytocin is used to induce spawning in the high-body amberjack broodstock, and the anti-inflammatory composition is used for post-spawning anti-inflammatory treatment.

[0039] In some embodiments, the oxytocin comprises a gonadotropin-releasing hormone agonist, dioxone, and an extract from the pituitary gland of the grouper.

[0040] In some embodiments, the oblique-banded grouper is a 3-4 year old oblique-banded grouper, preferably a 3-year-old oblique-banded grouper.

[0041] In some embodiments, the anti-inflammatory composition comprises:

[0042] (a) glucose and doxycycline hydrochloride; and / or

[0043] (b) Glucose, penicillin and complex amino acids.

[0044] In some embodiments, the anti-inflammatory composition comprises glucose and doxycycline hydrochloride, and is used for anti-inflammatory purposes in broodstock with no external damage.

[0045] In some embodiments, the anti-inflammatory composition comprises glucose, penicillin, and a complex of amino acids; the anti-inflammatory composition is used to treat severe injuries (i.e., obvious damage to the body surface) in broodstock.

[0046] In some embodiments, the raw materials for preparing the pre-spawning enhanced rearing feed for high-body yellowtail broodstock include miscellaneous fish, Antarctic krill, eel meal, flour, compound vitamins, traditional Chinese medicine, probiotic liquid, oysters, and fish oil. The traditional Chinese medicine includes hawthorn, privet fruit, salvia miltiorrhiza, dryopteris crassirhizoma, chuanxiong rhizome, and astragalus membranaceus.

[0047] In some embodiments, the probiotic solution contains Bacillus subtilis and Lactobacillus.

[0048] In some embodiments, the raw materials for preparing the pre-spawning enhanced rearing feed for high-body amberjack broodstock include 50-60 parts of miscellaneous fish, 14-16 parts of Antarctic krill, 14-16 parts of oysters, 9-11 parts of eel meal, 2-4 parts of fish oil, 1-5 parts of flour, 0.1-1 parts of compound vitamins, 0.1-1 parts of traditional Chinese medicine, and 1-3 parts of probiotic liquid, with a liquid concentration of 1×10⁻⁶. 6 -3×10 6 cfu / ml.

[0049] In some embodiments, the raw materials for preparing the pre-spawning enhanced rearing feed for high-body yellowtail broodstock, by weight, include 55 parts of miscellaneous fish, 14 parts of Antarctic krill, 14 parts of oysters, 10 parts of eel powder, 2 parts of fish oil, 4 parts of flour, 0.5 parts of compound vitamins, 0.5 parts of traditional Chinese medicine, and 2 parts of probiotic liquid.

[0050] In some embodiments, the raw materials for preparing the pre-spawning enhanced rearing feed for high-body yellowtail broodstock, by weight, include 52 parts of miscellaneous fish, 15 parts of Antarctic krill, 15 parts of oysters, 10 parts of eel powder, 4 parts of fish oil, 3 parts of flour, 0.5 parts of compound vitamins, 0.5 parts of traditional Chinese medicine, and 2 parts of probiotic liquid.

[0051] In some embodiments, the mass ratio of hawthorn, privet fruit, salvia miltiorrhiza, dryopteris crassirhizoma, chuanxiong rhizome, and astragalus root in the traditional Chinese medicine is 1:1:1:1:1:1.

[0052] In some embodiments, the probiotic solution comprises Bacillus subtilis and Lactobacillus, with a volume ratio of 1:1 and a concentration of 1×10⁻⁶ for both solutions. 6 -3×10 6 cfu / ml, preferably about 2×10 6 cfu / ml.

[0053] In some embodiments, the pre-spawning enhanced rearing feed for high-body yellowtail broodstock is a soft pellet feed.

[0054] In some embodiments, the soft pellet feed has a particle size of 1.4-1.6 cm and a length of 4-6 cm.

[0055] In some embodiments, the raw materials for preparing the high-body yellowtail broodstock postpartum care feed include miscellaneous fish, Antarctic krill, eel powder, flour, compound vitamins, traditional Chinese medicine, probiotic liquid, sandworm, squid paste, and compound amino acids. The traditional Chinese medicine includes hawthorn, Dryopteris crassirhizoma, Astragalus membranaceus, honeysuckle, licorice, and Codonopsis pilosula.

[0056] In some embodiments, the probiotic solution contains Bacillus subtilis and Lactobacillus.

[0057] In some embodiments, the raw materials for preparing the high-body yellowtail broodstock postpartum care feed include 55-65 parts of miscellaneous fish, 14-16 parts of Antarctic krill, 9-11 parts of sandworms, 9-11 parts of eel meal, 2-4 parts of squid paste, 1-5 parts of flour, 0.1-1 parts of compound vitamins, 0.1-1 parts of compound amino acids, 0.1-1 parts of traditional Chinese medicine, and 1-3 parts of probiotic liquid with a concentration of 1×10⁻⁶. 6 -3×10 6 cfu / ml.

[0058] In some embodiments, the raw materials for preparing the high-body yellowtail broodstock postpartum care feed, by weight, include 60 parts of miscellaneous fish, 14 parts of Antarctic krill, 9 parts of sandworms, 10 parts of eel powder, 2 parts of squid paste, 3 parts of flour, 0.5 parts of compound vitamins, 0.5 parts of compound amino acids, 1 part of traditional Chinese medicine, and 2 parts of probiotic liquid.

[0059] In some embodiments, the raw materials for preparing the high-body yellowtail broodstock postpartum care feed, by weight, include 57 parts of miscellaneous fish, 15 parts of Antarctic krill, 10 parts of sandworms, 10 parts of eel powder, 3 parts of squid paste, 3 parts of flour, 0.5 parts of compound vitamins, 0.5 parts of compound amino acids, 1 part of traditional Chinese medicine, and 2 parts of probiotic liquid.

[0060] In some embodiments, the mass ratio of hawthorn, Dryopteris crassirhizoma, Astragalus membranaceus, honeysuckle, licorice, and Codonopsis pilosula in the traditional Chinese medicine is 1:1:1:1:1:1.

[0061] In some embodiments, the probiotic solution comprises Bacillus subtilis and Lactobacillus, with a volume ratio of 1:1 and a concentration of 1×10⁻⁶ for both solutions. 6 -3×10 6 cfu / ml, preferably about 2×10 6 cfu / ml.

[0062] In some embodiments, the postpartum care feed for high-body yellowtail broodstock is a soft pellet feed.

[0063] In some embodiments, the soft pellet feed has a particle size of 1.4-1.6 cm and a length of 4-6 cm.

[0064] The third aspect of this invention provides the application of the combination of the feed of the first aspect and the reagent of the second aspect in the artificial breeding of high-body amberjack.

[0065] A fourth aspect of this invention provides a method for the artificial breeding of high-body amberjack, comprising the following steps:

[0066] Pre-spawning intensive rearing of broodstock: Broodstock are raised using high-body yellowtail pre-spawning intensive rearing feed;

[0067] Secondary ovulation induction: Secondary ovulation induction is performed on female parent fish that do not spawn or whose spawning rate does not exceed 50%.

[0068] Postpartum care: After the high-body amberjack broodstock have completed breeding (complete breeding means that all female broodstock have laid more than 50% of their eggs), use high-body amberjack broodstock postpartum care feed to raise the broodstock;

[0069] The raw materials for preparing the pre-spawning enhanced rearing feed for high-body yellowtail broodstock include miscellaneous fish, Antarctic krill, eel meal, flour, compound vitamins, traditional Chinese medicine, probiotic liquid, oysters, and fish oil. The traditional Chinese medicine includes hawthorn, privet fruit, salvia miltiorrhiza, dryopteris crassirhizoma, chuanxiong rhizome, and astragalus membranaceus.

[0070] In some embodiments, the probiotic solution contains Bacillus subtilis and Lactobacillus.

[0071] In some embodiments, the raw materials for preparing the pre-spawning enhanced rearing feed for high-body yellowtail broodstock include 50-60 parts of miscellaneous fish, 14-16 parts of Antarctic krill, 14-16 parts of oysters, 9-11 parts of eel meal, 2-4 parts of fish oil, 1-5 parts of flour, 0.1-1 parts of compound vitamins, 0.1-1 parts of traditional Chinese medicine, and 1-2 parts of probiotic liquid.

[0072] In some embodiments, the raw materials for preparing the pre-spawning enhanced rearing feed for high-body yellowtail broodstock, by weight, include 55 parts of miscellaneous fish, 14 parts of Antarctic krill, 14 parts of oysters, 10 parts of eel powder, 2 parts of fish oil, 4 parts of flour, 0.5 parts of compound vitamins, 0.5 parts of traditional Chinese medicine, and 2 parts of probiotic liquid.

[0073] In some embodiments, the raw materials for preparing the pre-spawning enhanced rearing feed for high-body yellowtail broodstock, by weight, include 52 parts of miscellaneous fish, 15 parts of Antarctic krill, 15 parts of oysters, 10 parts of eel powder, 4 parts of fish oil, 3 parts of flour, 0.5 parts of compound vitamins, 0.5 parts of traditional Chinese medicine, and 2 parts of probiotic liquid.

[0074] In some embodiments, the mass ratio of hawthorn, privet fruit, salvia miltiorrhiza, dryopteris crassirhizoma, chuanxiong rhizome, and astragalus root in the traditional Chinese medicine is 1:1:1:1:1:1.

[0075] In some embodiments, the probiotic solution comprises Bacillus subtilis and Lactobacillus, with a volume ratio of 1:1 and a concentration of 1×10⁻⁶ for both solutions. 6 -3×10 6 cfu / ml, preferably about 2×10 6 cfu / ml.

[0076] In some embodiments, the pre-spawning enhanced rearing feed for high-body yellowtail broodstock is a soft pellet feed.

[0077] In some embodiments, the soft pellet feed has a particle size of 1.4-1.6 cm and a length of 4-6 cm.

[0078] In some embodiments, the miscellaneous fish includes mackerel and triggerfish.

[0079] In some implementations, the pre-spawning intensive rearing phase for broodstock refers to the period from 60 days before spawning to the implementation of spawning induction.

[0080] By pre-spawning intensive breeding, high-body yellowtail broodstock are placed in habitat conditions that meet their reproductive needs in advance, reducing their stress response during induced spawning and improving the stability of broodstock reproduction.

[0081] In some implementations, the broodstock are raised for 3-5 days and then fed with pre-spawning intensive feed for high-body yellowtail broodstock. The average daily feed amount is 2-3% of the broodstock's body weight, and is gradually increased to 6%-7% of the broodstock's body weight depending on the feeding situation. The broodstock are fed 1-2 times a day.

[0082] For broodstock that are spawning normally and have laid more than 50% of their eggs, there is no need for a second ovulation induction. It is only necessary to ensure that the female broodstock have spawned all their eggs.

[0083] In some embodiments, the secondary ovulation induction includes gently pressing the abdomen of the female parent fish to expel the eggs, or injecting oxytocin into the female parent fish. If the genital opening is blocked, it should be punctured to prevent it from absorbing water and swelling.

[0084] In some embodiments, the oxytocin comprises a gonadotropin-releasing hormone agonist, dioxone, and an extract from the pituitary gland of the grouper.

[0085] In some embodiments, the oblique-banded grouper is a 3-4 year old oblique-banded grouper, preferably a 3-year-old oblique-banded grouper.

[0086] In some implementations, the injection dose of oxytocin during the secondary ovulation induction is 0.5 mL / kg of fish body weight.

[0087] In some embodiments, the oxytocin injected during the secondary ovulation induction contains 1 mg / mL of gonadotropin-releasing hormone agonist, 50 mg / mL of dioxone, and 1 mg / mL of pituitary extract from the grouper.

[0088] In some implementations, the secondary ovulation induction process also includes the step of introducing a healthy, disease-free, and unused male parent. Introducing a healthy, disease-free, and unused male parent can help with the secondary ovulation induction.

[0089] In some embodiments, the raw materials for preparing the high-body yellowtail broodstock postpartum care feed include miscellaneous fish, Antarctic krill, eel powder, flour, compound vitamins, traditional Chinese medicine, probiotic liquid, sandworm, squid paste, and compound amino acids. The traditional Chinese medicine includes hawthorn, Dryopteris crassirhizoma, Astragalus membranaceus, honeysuckle, licorice, and Codonopsis pilosula.

[0090] In some embodiments, the probiotic solution contains Bacillus subtilis and Lactobacillus.

[0091] In some embodiments, the raw materials for preparing the high-body yellowtail broodstock postpartum care feed include 55-65 parts of miscellaneous fish, 14-16 parts of Antarctic krill, 9-11 parts of sandworms, 9-11 parts of eel meal, 2-4 parts of squid paste, 1-5 parts of flour, 0.1-1 parts of compound vitamins, 0.1-1 parts of compound amino acids, 0.1-1 parts of traditional Chinese medicine, and 1-3 parts of probiotic liquid with a concentration of 1×10⁻⁶. 6 -3×106 cfu / ml.

[0092] In some embodiments, the feed preparation ingredients, by weight, include 60 parts of miscellaneous fish, 14 parts of Antarctic krill, 9 parts of sandworms, 10 parts of eel powder, 2 parts of squid paste, 3 parts of flour, 0.5 parts of compound vitamins, 0.5 parts of compound amino acids, 1 part of traditional Chinese medicine, and 2 parts of probiotic liquid.

[0093] In some embodiments, the feed preparation ingredients, by weight, include 57 parts of miscellaneous fish, 15 parts of Antarctic krill, 10 parts of sandworms, 10 parts of eel powder, 3 parts of squid paste, 3 parts of flour, 0.5 parts of compound vitamins, 0.5 parts of compound amino acids, 1 part of traditional Chinese medicine, and 2 parts of probiotic liquid.

[0094] In some embodiments, the mass ratio of hawthorn, Dryopteris crassirhizoma, Astragalus membranaceus, honeysuckle, licorice, and Codonopsis pilosula in the traditional Chinese medicine is 1:1:1:1:1:1.

[0095] In some embodiments, the probiotic solution comprises Bacillus subtilis and Lactobacillus, with a volume ratio of 1:1 and a concentration of 1×10⁻⁶ for both solutions. 6 -3×10 6 cfu / ml, preferably, is about 2 × 10⁻⁶. 6 cfu / ml.

[0096] In some embodiments, the postpartum care feed for high-body yellowtail broodstock is a soft pellet feed.

[0097] In some embodiments, the soft pellet feed has a particle size of 1.4-1.6 cm and a length of 4-6 cm.

[0098] In some embodiments, the miscellaneous fish include mackerel and triggerfish, such as blue trevally and devil triggerfish.

[0099] In some implementations, the high-body amberjack broodstock postpartum care feed is started 3-5 days after spawning. The daily feeding amount of the high-body amberjack broodstock postpartum care feed is 5-7% of the broodstock body weight, and the feeding amount is adjusted according to the broodstock's feeding behavior, with the goal of preventing the broodstock from competing for food. The feed is given 1-2 times a day.

[0100] In some implementations, the broodstock of high-bodied yellowtail are healthy, vigorous, and domesticated high-bodied yellowtail aged 3 years or older from Guangdong, Fujian, and other regions.

[0101] In some implementations, the high-body yellowtail broodstock are high-body yellowtail with a symmetrical body shape, smooth and uninjured body surface, robust physique, good feeding, strong vitality, no disease or deformity, body length ≥70cm, and weight ≥7kg.

[0102] In some implementations, the ratio of male to female broodstock in the high-bodied yellowtail is 1:1.

[0103] In some implementations, the broodstock of the high-bodied yellowtail are cultured in net cages in open sea areas with good currents, no surrounding pollution, gentle seabed topography, and a bottom sediment that is mainly muddy or sandy, far from the coastline.

[0104] In some implementations, the broodstock of the high-bodied yellowtail are cultured in net cages in sea areas with water flow ≤1.5m / s, water temperature ≥20℃, salinity 30‰-40‰, pH 8±0.5, dissolved oxygen ≥5mg / L, minimum tidal depth ≥12m, good water flow, small waves, and water quality that meets GB11607 standards.

[0105] In some implementations, the netting of the cages is made of polyethylene or nylon. When using knotted nets woven from polyethylene, it is advisable to attach a small amount of substrate in the sea before releasing the broodstock to reduce friction on their bodies. The cage size should be selected according to the size of the high-bodied amberjack broodstock, for example, a cage with dimensions of 10*10*5 cm. The mesh size should be such that the fish cannot escape, and therefore should be determined according to the size of the fish, for example, a mesh size of 3-6 cm.

[0106] In some implementations, the stocking density of high-bodied yellowtail in the net cage is ≤6 fish / 10m². 3 .

[0107] In some implementations, high-body yellowtail broodstock need to be sterilized and dewormed before pre-spawning intensive breeding.

[0108] In some implementations, sterilization and pest control are achieved through immersion. Specifically, the broodstock are immersed in seawater containing added hydrogen peroxide solution for disinfection and pest control. When the hydrogen peroxide solution concentration is 30% (mass concentration), 200-300 ml of hydrogen peroxide solution is added per cubic meter of seawater. If other concentrations of hydrogen peroxide solution are used, the volume of hydrogen peroxide solution to be added can be calculated according to this ratio. The broodstock are immersed for disinfection for 5-10 minutes before being placed in net cages for cultivation. Thereafter, the broodstock are immersed for disinfection once every 20 days.

[0109] In some embodiments, postpartum care also includes an anti-inflammatory and disinfection treatment of the broodstock. Specifically, the broodstock are examined, and those without external injuries are injected with glucose and doxycycline hydrochloride for internal anti-inflammatory treatment (the injection dose of glucose is 0.5 ml per kilogram of body weight, and the injection dose of doxycycline hydrochloride is 1 mg per kilogram of body weight). Severely injured fish are injected with glucose, penicillin, and compound amino acids (the injection dose of glucose is 0.5 ml per kilogram of body weight, the injection dose of penicillin is 100,000 units per kilogram of body weight, and the injection dose of compound amino acids is 20 mg per kilogram of body weight). Afterward, all broodstock are immersed in seawater containing hydrogen peroxide solution for 5-10 minutes for disinfection. Veterinary streptomycin or penicillin is added to the solution to achieve better anti-inflammatory and wound-healing effects. When the hydrogen peroxide solution concentration is 30% (mass concentration), 200-300 ml of hydrogen peroxide solution is added per cubic meter of seawater. If other concentrations of hydrogen peroxide solution are used, the volume of hydrogen peroxide solution to be added can be calculated according to this ratio.

[0110] In some implementations, during postpartum care, the health status of the parent fish can be checked regularly to detect and treat diseases in a timely manner. When taking samples, try to keep them in water to reduce unnecessary damage to the fish. Regularly check the parent fish for any abnormal symptoms to ensure early detection, early diagnosis, and early treatment, prevent the spread of diseases, and promptly remove dead fish for harmless disposal.

[0111] The beneficial effects of this invention are as follows: This application provides a high-body amberjack broodstock feed containing traditional Chinese medicine. By combining different raw materials, the high-body amberjack broodstock feed can better provide nutrients and active substances to the amberjack broodstock. Before spawning, it can enhance the physical strength and immunity of the broodstock, promote their development, accelerate sexual maturity, and increase the spawning rate, egg production, fertilization rate, and hatching rate. After spawning, it can promote wound healing and recovery of the broodstock, ensuring the survival rate and quality of the broodstock after spawning.

[0112] This invention also provides a breeding method using high-body amberjack broodstock feed. By strengthening the broodstock before spawning, the stress of induced spawning is reduced, and the stability of broodstock reproduction is improved. Furthermore, by feeding high-body amberjack broodstock feed, the physical strength and immunity of the broodstock are enhanced, promoting the development of the broodstock, accelerating sexual maturity, and increasing the induced spawning rate, egg production, fertilization rate, and hatching rate. Detailed Implementation

[0113] The present invention will be further described in detail below through specific embodiments. Unless otherwise specified, the raw materials, reagents, or apparatus used in the embodiments and comparative examples are all available from conventional commercial sources or can be obtained by existing technical methods. Unless otherwise specified, the test or experimental methods are conventional methods in the art.

[0114] The compound vitamins used in this embodiment of the invention are instant electrolytic multivitamins produced by Jiangsu Aotewo Aquatic Technology Co., Ltd., with product number A30KY; the compound amino acids used in this embodiment of the invention are soybean compound amino acid powder (80%) produced by Sichuan Mianzhu Ruiyang Biotechnology Co., Ltd.

[0115] Example 1

[0116] Thirty healthy, vigorous, and vigorous amberjacks with well-developed gonads, a body length ≥70cm, a weight ≥7kg, and a female-to-male ratio of 1:1, aged 3 years, were selected as broodstock. They were placed in 10*10*5 cm net cages made of nylon material for rearing, starting approximately 60 days before spawning and continuing until spawning induction. The net cages were placed in the sea area of ​​Qixia Village, Dongshan County, Fujian Province, where the water current is ≤1.2m / s, the water temperature is ≥21℃, the salinity is ≥30‰, the pH is 8±0.5, the dissolved oxygen is ≥6mg / L, the lowest tidal depth is ≥12m, and the distance from the shore is approximately 4km. After selection, the high-body yellowtail broodstock are first soaked in a water bag for disinfection. The water bag contains seawater with added hydrogen peroxide solution. 200ml of hydrogen peroxide solution is added per cubic meter of seawater, and the concentration of hydrogen peroxide solution is 30% (mass concentration). The broodstock are soaked for disinfection for 10 minutes before being placed in a net cage for cultivation. After that, the broodstock are soaked for disinfection once every 20 days.

[0117] Three days after stocking the broodstock, feeding begins. The initial feeding amount is approximately 3% of the broodstock's body weight. At this point, the broodstock cease competing for food. On the fifth day, the feeding amount is increased to approximately 6% of the broodstock's body weight, at which point the competition stops. From then until the breeding season, the feeding amount remains at 6-7% of the broodstock's body weight. The feed consists of the following components by weight percentage: 55 parts miscellaneous fish (including bluefin trevally and devil triggerfish), 14 parts Antarctic krill, 14 parts oysters, 10 parts eel meal, 2 parts fish oil, 4 parts flour, 0.5 parts multivitamins, and 0.5 parts traditional Chinese medicine (hawthorn, privet fruit, salvia miltiorrhiza, dryopteris crassirhizoma, chuanxiong rhizome, astragalus membranaceus). Each component of the feed is first ground separately. The ground miscellaneous fish, Antarctic krill, and oysters are then mixed together. The mixture is placed in a fermentation tank, and 2 parts of probiotic liquid (including Bacillus subtilis liquid and Lactobacillus liquid, with a volume ratio of 1:1 and a concentration of approximately 2×10⁻⁶ for both liquids) are added. 6 Add cfu / ml) to the fermentation tank and mix it evenly with the mixture. Adjust the temperature of the fermentation tank to 35-40℃ and ferment for 60 hours. After that, mix the fermented mixture evenly with eel powder, fish oil, flour, compound vitamins and Chinese herbal powder. Then, extrude it into soft pellet feed with a particle size of 1.4-1.6cm and a length of 4-6cm through a pellet mill.

[0118] Artificial spawning is induced. After the parent fish have finished spawning, check the female parent fish for spawning, whether the abdomen is swollen, whether the outline of the ovary is obvious, and whether a small number of eggs flow out when the vent is gently squeezed. For female broodstock with high spawning rates, once the eggs have been largely released, no further ovulation induction is performed. For broodstock that do not spawn or whose spawning rate is less than 50%, the female broodstock is removed and the eggs are gently squeezed out by pressing on their abdomen. For female broodstock that cannot be squeezed out, ovulation-inducing hormones are injected again. The concentration of gonadotropin-releasing hormone agonist (GnRHa) in the ovulation induction agent is 1 mg / ml, the concentration of domperidone (DOM) is 50 mg / ml, and the concentration of pituitary extract of *Sinocyclocheilus fasciatus* is 1 mg / ml. The volume ratio of the three is GnRHa:DOM:3-year-old *Sinocyclocheilus fasciatus* pituitary extract = 3:6:1. The injection dose is 0.5 mL / kg of fish body weight. A second ovulation induction is performed to ensure that the female broodstock's eggs are largely released. During the second ovulation induction process, a healthy, disease-free, and unused male broodstock is introduced to help the fish release its eggs completely.

[0119] The broodstock were examined. Fish with no external injuries were injected internally with glucose and doxycycline hydrochloride for anti-inflammatory purposes. The glucose concentration was 0.5 ml per kilogram of body weight, and the doxycycline hydrochloride concentration was 1 mg per kilogram of body weight. Fish with severe injuries were injected internally with a mixture of glucose, penicillin, and compound amino acids. The glucose concentration was 0.5 ml per kilogram of body weight, the penicillin concentration was 100,000 units per kilogram of body weight, and the compound amino acids concentration was 20 mg per kilogram of body weight. After that, all the broodstock were immersed in seawater with added hydrogen peroxide solution for disinfection for 10 minutes. 200 ml of hydrogen peroxide solution (30% hydrogen peroxide concentration) was added per cubic meter of seawater, and 5 million units of veterinary streptomycin or penicillin per liter of seawater were added to the solution to achieve better anti-inflammatory and wound healing effects.

[0120] After 3 days of temporary rearing (without feeding during this period), start feeding small amounts of food, feeding frequently in small amounts each day. The feed consists of the following components by weight percentage: 60 parts miscellaneous fish (including bluefin trevally and devil triggerfish), 14 parts Antarctic krill, 9 parts sandworms, 10 parts eel meal, 2 parts squid paste, 3 parts flour, 0.5 parts compound vitamins, 0.5 parts compound amino acids, and 1 part traditional Chinese medicine (hawthorn, Dryopteris crassirhizoma, Astragalus membranaceus, honeysuckle, licorice, and Codonopsis pilosula). First, grind each component of the feed separately. Then, mix the ground miscellaneous fish, Antarctic krill, and sandworms together. Place the mixture in a fermentation tank and add 2 parts of probiotic liquid (including Bacillus subtilis liquid and Lactobacillus liquid, with a volume ratio of 1:1 and a concentration of approximately 2 × 10⁻⁶ for both liquids). 6Add cfu / ml to the fermentation tank and mix evenly with the mixture. Control the temperature of the fermentation tank at 35-40℃ and ferment for 60 hours. After that, mix the fermented mixture evenly with eel powder, squid paste, flour, compound vitamins, compound amino acids and Chinese herbal medicine powder. Extrude the mixture into soft pellet feed with a particle size of 1.4-1.6cm and a length of 4-6cm through a pellet mill to enhance immunity and promote postpartum recovery. The daily feeding amount should be gradually transitioned to 5-7% of the broodstock's body weight according to the feeding situation of the broodstock, and continue feeding for 30 days.

[0121] During the postpartum care of high-body yellowtail broodstock, regularly (every 15 days) a 30% hydrogen peroxide solution was used. 200ml of hydrogen peroxide solution was added per cubic meter of seawater, and the broodstock were immersed in the solution for 10 minutes for disinfection to prevent bacterial diseases. The broodstock were regularly checked for any abnormal symptoms to ensure early detection, diagnosis, and treatment, preventing the spread of disease. Dead fish were promptly removed and disposed of harmlessly. One dead fish was observed in this example.

[0122] A total of 30 broodstock fish were selected for this experiment. No broodstock fish were lost during the pre-spawning rearing period. Of the 15 females, 7 were successfully induced to spawn, resulting in a spawning rate of 46.7%. Approximately 2.51 million eggs were fertilized, bringing the total egg count to approximately 5.18 million, with a fertilization rate of 48.6%. Approximately 1.87 million fry hatched, resulting in a hatching rate of 74.2%. After completing reproduction and undergoing 30 days of post-spawning care, a total of 29 broodstock fish survived, representing a survival rate of 96.7%.

[0123] Example 2

[0124] Thirty healthy, vigorous, and vigorous amberjacks with well-developed gonads, a body length ≥70cm, a weight ≥7kg, and a female-to-male ratio of 1:1, aged 3 years, were selected as broodstock. They were placed in 10*10*5 cm net cages made of nylon material for rearing, starting approximately 60 days before spawning and continuing until spawning induction. The net cages were placed in the sea area of ​​Qixia Village, Dongshan County, Fujian Province, where the water current is ≤1.2m / s, the water temperature is ≥21℃, the salinity is ≥30‰, the pH is 8±0.5, the dissolved oxygen is ≥6mg / L, the lowest tidal depth is ≥12m, and the distance from the shore is approximately 4km. After selection, the high-body yellowtail broodstock are first soaked in a water bag for disinfection. The water bag contains seawater with added hydrogen peroxide solution. 300ml of hydrogen peroxide solution is added per cubic meter of seawater, and the concentration of hydrogen peroxide solution is 30% (mass concentration). The broodstock are soaked for disinfection for 10 minutes before being placed in a net cage for cultivation. After that, the broodstock are soaked for disinfection once every 20 days.

[0125] Three days after stocking the broodstock, feeding begins. The initial feeding amount is approximately 3% of the broodstock's body weight. At this point, the broodstock cease competing for food. On the fifth day, the feeding amount is increased to approximately 6% of the broodstock's body weight, at which point the competition stops. From then until the breeding season, the feeding amount remains at 6-7% of the broodstock's body weight. The feed consists of the following components by weight percentage: 52 parts miscellaneous fish (including bluefin trevally and devil triggerfish), 15 parts Antarctic krill, 15 parts oysters, 10 parts eel meal, 4 parts fish oil, 3 parts flour, 0.5 parts multivitamins, and 0.5 parts traditional Chinese medicine (hawthorn, privet fruit, salvia miltiorrhiza, dryopteris crassirhizoma, chuanxiong rhizome, astragalus membranaceus). Each component of the feed is first ground separately. The ground miscellaneous fish, Antarctic krill, and oysters are then mixed together. The mixture is placed in a fermentation tank, and a certain amount of probiotic liquid (including Bacillus subtilis liquid and Lactobacillus liquid, with a volume ratio of 1:1 and a concentration of approximately 2 × 10⁻⁶ for both liquids) is added. 6 Add cfu / ml) to the fermentation tank and mix it evenly with the mixture. Adjust the temperature of the fermentation tank to 35-40℃ and ferment for 60 hours. After that, mix the fermented mixture evenly with eel powder, fish oil, flour, compound vitamins and Chinese herbal powder. Then, extrude it into soft pellet feed with a particle size of 1.4-1.6cm and a length of 4-6cm through a pellet mill.

[0126] Artificial spawning is induced. After the parent fish have finished spawning, check the female parent fish for spawning, whether the abdomen is swollen, whether the outline of the ovary is obvious, and whether a small number of eggs flow out when the vent is gently squeezed. For female broodstock with high spawning rates, once the eggs have been largely released, no further ovulation induction is performed. For broodstock that do not spawn or whose spawning rate is less than 50%, the female broodstock is removed and the eggs are gently squeezed out by pressing on their abdomen. For female broodstock that cannot be squeezed out, ovulation-inducing hormones are injected again. The concentration of gonadotropin-releasing hormone agonist (GnRHa) in the ovulation induction agent is 1 mg / ml, the concentration of domperidone (DOM) is 50 mg / ml, and the concentration of pituitary extract of *Sinocyclocheilus fasciatus* is 1 mg / ml. The volume ratio of the three is GnRHa:DOM:3-year-old *Sinocyclocheilus fasciatus* pituitary extract = 3:6:1. The injection dose is 0.5 mL / kg of fish body weight. A second ovulation induction is performed to ensure that the female broodstock's eggs are largely released. During the second ovulation induction process, a healthy, disease-free, and unused male broodstock is introduced to help the fish release its eggs completely.

[0127] The broodstock were examined. Fish with no external injuries were injected with glucose and doxycycline hydrochloride for internal anti-inflammatory treatment. The glucose concentration was 0.5 ml per kilogram of body weight, and the doxycycline hydrochloride concentration was 1 mg per kilogram of body weight. Fish with severe injuries were injected with a mixture of glucose, penicillin, and compound amino acids. The glucose concentration was 0.5 ml per kilogram of body weight, the penicillin concentration was 100,000 units per kilogram of body weight, and the compound amino acids concentration was 20 mg per kilogram of body weight. Afterward, all the broodstock were immersed in seawater with added hydrogen peroxide solution for disinfection for 10 minutes. 200 ml of hydrogen peroxide solution (30% hydrogen peroxide concentration) was added per cubic meter of seawater. 5 million units of veterinary streptomycin or penicillin per liter of seawater were added to the solution to achieve better anti-inflammatory and wound-healing effects.

[0128] After 3 days of temporary rearing, start feeding small amounts of feed (do not feed during the temporary rearing period), feeding in small amounts frequently throughout the day. The feed consists of the following components by weight percentage: 57 parts miscellaneous fish (including bluefin trevally and devil triggerfish), 15 parts Antarctic krill, 10 parts sandworms, 10 parts eel meal, 3 parts squid paste, 3 parts flour, 0.5 parts compound vitamins, 0.5 parts compound amino acids, and 1 part traditional Chinese medicine (hawthorn, Dryopteris crassirhizoma, Astragalus membranaceus, honeysuckle, licorice, and Codonopsis pilosula). First, grind each component of the feed separately. Then, mix the ground miscellaneous fish, Antarctic krill, and sandworms together. Place the mixture in a fermentation tank and add 2 parts of probiotic liquid (including Bacillus subtilis liquid and Lactobacillus liquid, with a volume ratio of 1:1 and a concentration of approximately 2×10⁻⁶ for both liquids). 6 Add cfu / ml to the fermentation tank and mix evenly with the mixture. Control the temperature of the fermentation tank at 35-40℃ and ferment for 60 hours. After that, mix the fermented mixture evenly with eel powder, squid paste, flour, compound vitamins, compound amino acids and Chinese herbal medicine powder. Extrude the mixture into soft pellet feed with a particle size of 1.4-1.6cm and a length of 4-6cm through a pellet mill to enhance immunity and promote postpartum recovery. The daily feeding amount should be gradually transitioned to 5-7% of the broodstock's body weight according to the feeding situation of the broodstock, and continue feeding for 30 days.

[0129] During the postpartum care of high-body yellowtail broodstock, regularly (every 15 days) a 30% hydrogen peroxide solution was used. 300 ml of hydrogen peroxide solution was added per cubic meter of seawater, and the broodstock were immersed in the solution for 10 minutes for disinfection to prevent bacterial diseases. The broodstock were regularly checked for any abnormal symptoms to ensure early detection, diagnosis, and treatment, preventing the spread of disease. Dead fish were promptly removed and disposed of harmlessly. No fish deaths occurred in this embodiment.

[0130] A total of 30 broodstock fish were selected for this experiment. No broodstock fish were lost during the pre-spawning rearing period. Of the 15 females, 8 were successfully induced to spawn, resulting in a spawning rate of 53.3%. Approximately 3.05 million eggs were fertilized, bringing the total egg count to approximately 5.83 million, with a fertilization rate of 52.4%. Approximately 2.39 million fry hatched, resulting in a hatching rate of 74.2%. After completing reproduction and undergoing 30 days of post-spawning care, all 30 broodstock fish survived, achieving a survival rate of 100%.

[0131] Comparative Example 1

[0132] Thirty healthy, vigorous, and vigorous amberjacks with well-developed gonads, a body length ≥70cm, a weight ≥7kg, and a female-to-male ratio of 1:1, aged 3 years, were selected as broodstock. They were placed in 10*10*5 cm net cages made of nylon material for rearing, starting approximately 60 days before spawning and continuing until spawning induction. The net cages were placed in the sea area of ​​Qixia Village, Dongshan County, Fujian Province, where the water current is ≤1.2m / s, the water temperature is ≥21℃, the salinity is ≥30‰, the pH is 8±0.5, the dissolved oxygen is ≥6mg / L, the lowest tidal depth is ≥12m, and the distance from the shore is approximately 4km. After selection, the high-body yellowtail broodstock are first soaked in a water bag for disinfection. The water bag contains seawater with added hydrogen peroxide solution. 300ml of hydrogen peroxide solution is added per cubic meter of seawater, and the concentration of hydrogen peroxide solution is 30% (mass concentration). The broodstock are soaked for disinfection for 10 minutes before being placed in a net cage for cultivation. After that, the broodstock are soaked for disinfection once every 20 days.

[0133] Three days after stocking the broodstock, feeding begins. The initial feeding amount is about 3% of the broodstock's body weight. At this point, the broodstock stop competing for food. On the fifth day, the feeding amount is increased to about 6% of the broodstock's body weight, and the competition for food stops. Thereafter, until the breeding season, the feeding amount is 6-7% of the broodstock's body weight. The feed is frozen fish.

[0134] Artificial spawning was induced. After the parent fish had finished spawning, all broodstock were immersed in seawater containing added hydrogen peroxide solution for 10 minutes for disinfection. 200ml of hydrogen peroxide solution (30% concentration) was added per cubic meter of seawater, and 5 million units of veterinary streptomycin or penicillin per liter of seawater were added to the solution for better anti-inflammatory and wound-healing effects. After 3 days of temporary rearing, small amounts of food were introduced, fed frequently throughout the day. The food was fresh fish, and the daily feeding amount was gradually increased to 5-7% of the parent fish's body weight, depending on their feeding behavior, for 30 days.

[0135] A total of 30 broodstock fish were selected for this experiment. No broodstock fish were lost during the pre-spawning rearing period. Of the 15 females, 5 were successfully induced to spawn, resulting in a spawning rate of 33.3%. Approximately 1.4 million eggs were fertilized, bringing the total egg count to approximately 3.2 million, resulting in a fertilization rate of 43.7%. Approximately 970,000 fry hatched, resulting in a hatching rate of 69.7%. After completing reproduction and undergoing 30 days of post-spawning care, a total of 26 broodstock fish survived, resulting in a survival rate of 86.7%.

[0136] Table 1. Statistical results of spawning induction rate, egg production, fertilization rate, hatching rate, and postpartum broodstock survival rate in Examples 1-2 and Comparative Example 1.

[0137]

[0138] As shown in Table 1, Comparative Example 1 did not undergo pre-spawning nutritional fortification or post-spawning secondary ovulation induction or increased nutrient supply after being soaked and disinfected and placed in net cages for cultivation. Therefore, compared with Examples 1-2, the spawning rate, egg production, fertilization rate, hatching rate and post-spawning survival rate of parent fish were significantly lower.

[0139] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. A feed for high body grouper broodstock, wherein the feed is prepared from the following ingredients: miscellaneous fish, Antarctic krill, eel powder, flour, compound vitamin, Chinese herbal medicine and probiotic bacteria liquid. The Chinese herbal medicine comprises hawthorn, privet fruit, salvia miltiorrhiza, cyrtomium fortunei, chuanxiong and astragalus root, and the mass ratio of the six ingredients is 1:1:1:1:1:

1. Alternatively, the Chinese herbal medicine comprises hawthorn, cyrtomium fortunei, astragalus root, honeysuckle, licorice and dangshen, and the mass ratio of the six ingredients is 1:1:1:1:1:

1.

2. The feed according to claim 1, characterized in that, The probiotic bacteria liquid comprises bacillus subtilis and lactobacillus.

3. The feed of claim 1, characterized in that, The feed is soft granular feed.

4. The feed according to any one of claims 1 - 3, c h a ra cte ri zed i n that The preparation raw materials of the feed further comprise oysters and fish oil; the preparation raw materials of the feed comprise 50-60 parts of miscellaneous fish, 14-16 parts of Antarctic krill, 14-16 parts of oysters, 9-11 parts of eel powder, 2-4 parts of fish oil, 1-5 parts of flour, 0.1-1 parts of compound vitamins, 0.1-1 parts of Chinese herbal medicine, 1-3 parts of probiotic bacteria liquid, and the concentration of the bacteria liquid is 1x10 6 -3x10 6 cfu / ml. -3x10 6 cfu / ml.

5. The feed according to any one of claims 1-3, wherein the raw materials for preparing the feed further comprise polychaete, squid paste and compound amino acid; the raw materials for preparing the feed comprise 55-65 parts of miscellaneous fish, 14-16 parts of Antarctic krill, 9-11 parts of polychaete, 9-11 parts of eel powder, 2-4 parts of squid paste, 1-5 parts of flour, 0.1-1 part of compound vitamin, 0.1-1 part of compound amino acid, 0.1-1 part of Chinese herbal medicine, 1-3 parts of probiotic bacteria liquid, and the concentration of the bacteria liquid is 1 x 10 6 -3 x 10 6 cfu / ml.

5. The feed according to any one of claims 1-3, wherein the raw materials for preparing the feed further comprise polychaete, squid paste and compound amino acid; the raw materials for preparing the feed comprise 55-65 parts of miscellaneous fish, 14-16 parts of Antarctic krill, 9-11 parts of polychaete, 9-11 parts of eel powder, 2-4 parts of squid paste, 1-5 parts of flour, 0.1-1 part of compound vitamin, 0.1-1 part of compound amino acid, 0.1-1 part of Chinese herbal medicine, 1-3 parts of probiotic bacteria liquid, and the concentration of the bacteria liquid is 1 x 10 6.A reagent combination for artificial breeding of high body grouper, comprising oxytocin, anti-inflammatory composition and the feed according to any one of claims 1-5. The oxytocin comprises gonadotropin-releasing hormone agonist, diosgenin and epinephelus lanceolatus pituitary extract. The anti-inflammatory composition comprises: (a) glucose and doxycycline hydrochloride; and / or (b) glucose, penicillin and compound amino acid. 7.Use of the feed according to any one of claims 1-5 and the reagent combination according to claim 6 in artificial breeding of high body grouper. 8.A method for artificial breeding of high body grouper, comprising the following steps: Prepartum intensive cultivation of broodstock: using prepartum intensive cultivation feed for high body grouper broodstock to culture the broodstock; Secondary ovulation: performing secondary ovulation on female broodstock that do not spawn or spawn less than 50% of eggs; Postpartum care: using postpartum care feed for high body grouper broodstock to culture the broodstock after the broodstock finishes spawning; The prepartum intensive cultivation feed for high body grouper broodstock is prepared from the following ingredients: miscellaneous fish, Antarctic krill, eel powder, flour, compound vitamin, Chinese herbal medicine, probiotic bacteria liquid, oyster and fish oil, and the Chinese herbal medicine comprises hawthorn, privet fruit, salvia miltiorrhiza, cyrtomium fortunei, chuanxiong and astragalus root. The postpartum care feed for high body grouper broodstock is prepared from the following ingredients: miscellaneous fish, Antarctic krill, sandworm, eel powder, squid paste, flour, compound vitamin, compound amino acid, Chinese herbal medicine and probiotic bacteria liquid, and the Chinese herbal medicine comprises hawthorn, cyrtomium fortunei, astragalus root, honeysuckle, licorice and dangshen.

9. The artificial breeding method according to claim 8, characterized in that, The secondary ovulation comprises gently pressing the abdomen of the female broodstock to squeeze out the eggs, or injecting oxytocin into the female broodstock, and the oxytocin comprises gonadotropin-releasing hormone agonist, diosgenin and epinephelus lanceolatus pituitary extract.

Images