Small animal kidney capsule cell storage and transplantation device

Abstract

The present invention discloses a cell storage and transplantation device for small animals under the renal capsule, and relates to the field of experimental instruments for islet transplantation. The cell storage and transplantation device for small animals under the renal capsule includes two major parts: a storage tube and a storage box. The storage tube includes an outer tube and an inner tube. The inner side of the storage box ice box is provided with six placement slots. The device provided by the present invention can directly store cell suspensions. The storage tube can be directly placed in a low-speed centrifuge for centrifugation. The cells will settle to the lower section of the inner tube of the storage tube. The storage tube with centrifuged cells can be directly placed in a matching storage box for storage and transportation. The cells do not need to be transferred before cell transplantation, and the cell viability is greatly improved. The inner tube of the storage tube is compatible with the size of a 10μL pipette, and the transplantation can be directly performed with a 10μL pipette. When injecting, the round closed end of the gel at the end of the lower section of the inner tube of the storage tube is peeled off, and the cell transplantation can be performed directly.

Description

Technical Field

[0001] The invention relates to the field of experimental instruments, and more specifically to an inoculation device for cell transplantation under the kidney capsule of small animals. Background Art

[0002] Since the discovery of insulin in 1920, continuous technological advances have helped patients with type 1 diabetes to strictly control blood sugar changes in their bodies. However, exogenous insulin therapy cannot completely replace the physiological level of insulin secretion, and exogenous insulin therapy alone has great limitations. In 2000, Dr. Shapiro and his transplant team in Edmonton, Canada, successfully performed human islet transplantation, allowing many diabetic patients to successfully get rid of long-term dependence on exogenous insulin. Islet transplantation has become a new method for treating diabetes and has attracted widespread attention worldwide.

[0003] In clinical practice, the liver is generally chosen for islet transplantation in diabetic patients. However, in preclinical experiments, when mice or rats are selected as research subjects, the narrow field of view during portal vein injection, thin portal vein wall and difficulty in stopping bleeding after injection may cause graft loss. The renal capsule has a rich blood supply and is a closed environment that can provide sufficient nutrition and oxygen for the graft and prevent graft loss to a certain extent. Therefore, the renal capsule is generally the first choice for islet transplantation.

[0004] However, the renal capsule transplantation tools currently used, such as pointed forceps, injection needles, trocars, glass rods, etc., can easily puncture the renal capsule or damage the renal parenchyma, causing massive renal bleeding. In addition, it is difficult to ensure strict sterility when transferring islet cells to the injection hose in the early stage. It is also difficult to ensure the activity of the cells when the cells in the hose are transferred before transplantation. Therefore, a storage and transplantation device for islet cells in the renal capsule is proposed to solve the above problems. Summary of the invention

[0005] The present invention aims to address the problem that the existing tools cannot guarantee the activity of islet cells during transportation and the integrity of the kidney during transplantation when performing islet transplantation surgery in a diabetic model in a small animal model. The present invention proposes a new cell storage and transplantation device for transplantation in the renal capsule of small animals, thereby being able to transfer cells into the renal capsule with high activity and integrity without causing rupture of the renal capsule or damage to the renal parenchyma.

[0006] The technical solution disclosed in the present invention is as follows:

[0007] A cell storage and transplantation device for cell transplantation under the renal capsule of small animals, comprising a storage tube and a storage box:

[0008] The storage tube comprises an outer tube and an inner tube, the outer tube comprises a tube cover and a tube body, a circular cotton core is installed on the upper side of the inner part of the tube cover, the cotton core matches the size of the inner tube connecting section, a circular hole groove is arranged at the tube mouth of the outer tube, the tube body of the outer tube comprises an upper section and a lower section, the upper section is a cylindrical tube, the lower section is a conical tube which is larger at the top and smaller at the bottom, the upper section is connected to the large end of the conical tube of the lower section, and the small end of the conical tube of the lower section is a flat bottom; the inner tube of the storage tube comprises a connecting section and a lower part for storing cells, the connecting section is adapted to the size of the connecting part of a 10 μL pipette, the lower part of the storage cells comprises an upper section and a lower section, the upper section is a conical tube which is larger at the top and smaller at the bottom, the lower section is a cylindrical tube, the small end of the conical tube of the upper section is connected to the lower section, and the terminal end of the lower section is provided with a circular closed end of gel.

[0009] A box cover is installed on the top of the storage box, a fixing pad is fixedly installed on the inner top end of the box cover, an ice box is installed on the inner side of the storage box, and six placement grooves are opened on the inner side of the ice box, and the placement grooves are cylindrical placement grooves.

[0010] Furthermore, the diameter of the circular hole groove of the outer tube opening is slightly smaller than the diameter of the inner tube connecting section of the storage tube.

[0011] Furthermore, the lengths of the outer tube and the inner tube are 60-70 mm.

[0012] Furthermore, the upper section of the outer tube and the lower section of the outer tube are integrally formed.

[0013] Furthermore, the storage tube inner tube connecting section, the lower section of the storage inner tube and the terminal gel circular closed end of the lower section of the storage inner tube are integrally formed.

[0014] Furthermore, the diameter of the storage box placement groove is slightly larger than the diameter of the storage tube, and the diameter of the storage box placement groove is slightly smaller than the diameter of the tube cover of the storage tube.

[0015] Furthermore, the round closed end of the gel at the end of the lower section of the storage inner tube is easy to remove without leaving any residual gel.

[0016] Furthermore, both the inner tube and the outer tube of the storage tube are sterilized at high temperature and can be directly used to store cells.

[0017] Compared with the prior art, the present invention provides a cell storage and transplantation device for cell transplantation under the renal capsule of small animals, which has the following beneficial effects:

[0018] 1. The inner tube of the cell storage tube for renal capsule transplantation has been sterilized at high temperature and can directly store cells. The cell suspension can be directly added to the inner tube of the cell storage tube. After the outer tube cover of the storage tube is covered, the entire storage tube can be directly placed in a low-speed centrifuge for centrifugation. The cells will settle to the lower section of the inner tube of the storage tube. Then, the storage tube with centrifuged cells can be directly placed in a storage box with an ice box for storage and transportation until the cell transplantation. The cells do not need to be transferred during the whole process, the cell viability is greatly improved, and the waste of cells when transferred to the injection hose is avoided.

[0019] 2. The connecting section of the inner tube of the cell storage tube for renal capsule transplantation is compatible with the size of the connecting part of the 10μL pipette, so the renal capsule transplantation of cells can be directly performed with a 10μL pipette. During injection, the inner tube of the storage tube is connected with a 10μL pipette, and then the circular closed end of the gel at the end of the lower section of the inner tube of the storage tube is peeled off, that is, the end of the lower section of the inner tube of the storage tube can be directly inserted into the renal capsule. By pressing the pipette, the cells can be directly transplanted, so that the cells do not undergo other treatments from treatment to transplantation, thereby achieving the effect of improving the survival rate of cells before transplantation. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 A schematic diagram of the structure of a storage tube of a cell storage and transplantation device for cell transplantation under the renal capsule of small animals provided by the present invention;

[0021] Figure 2 A schematic diagram of the structure of a storage box of a cell storage and transplantation device for cell transplantation under the renal capsule of small animals provided by the present invention;

[0022] Numbers in the figure: 1, tube cover; 2, cotton core; 4, tube body; 401, upper section of outer tube; 402, lower section of outer tube; 403, lower end; 5, inner tube; 501, connecting section; 502, upper section of inner tube; 503, lower section of inner tube; 504, round closed end of terminal gel; 6, outer tube; 601, outer tube mouth; 10, lower part of cell storage 8, box cover; 801, fixing pad; 802, ice box; 901, placement slot; DETAILED DESCRIPTION

[0023] The present invention is further described below in conjunction with the accompanying drawings and embodiments, which are intended to explain the present invention rather than to limit it. Figure 1 and Figure 2 , a cell storage and transplantation device for cell transplantation under the renal capsule of small animals, comprising a storage tube ( Figure 1 ) and storage boxes ( Figure 2 ) Two parts:

[0024] The storage tube comprises an outer tube 6 and an inner tube 5, the lengths of the outer tube 6 and the inner tube 5 are 60-70 mm, the outer tube 6 comprises a tube cover 1 and a tube body 4, a round cotton core 2 is installed on the upper side of the tube cover 1, the cotton core 2 matches the size of the connecting section 501 of the inner tube 6, a round hole groove is arranged at the tube mouth 601 of the outer tube, the diameter of the round hole groove of the tube mouth 601 of the outer tube is slightly smaller than the diameter of the connecting section 501 of the inner tube 5 of the storage tube, the tube body 4 of the outer tube 6 comprises an upper section 401 and a lower section 402, the upper section 401 is a cylindrical tube, the lower section 402 is a tapered tube with a larger upper part and a smaller lower part, the upper section 401 is connected to the large end of the tapered tube of the lower section 402, and the small end 403 of the tapered tube of the lower section 402 is a flat bottom;

[0025] The storage tube inner tube 5 includes a connecting section 501 and a lower part 10 for storing cells, the connecting section 501 is adapted to the size of the connecting part of a 10 μL pipette, the lower part 10 for storing cells includes an upper section 502 and a lower section 503, the upper section 502 is a conical tube that is larger at the top and smaller at the bottom, the lower section 503 is a cylindrical tube, the small end of the conical tube of the upper section 502 is connected to the lower section 503, the terminal end of the lower section 503 is provided with a gel circular closed end 504, the terminal gel circular closed end 504 of the lower section 503 of the storage inner tube 5 is easy to remove and does not leave any residual glue, the storage tube inner tube 5 connecting section 501, the lower section 503 of the storage inner tube 5 and the terminal gel circular closed end 504 of the lower section 503 of the storage inner tube 5 are integrally formed. A box cover 8 is installed on the top of the storage box, and a fixing pad 801 is fixedly installed on the inner top end of the box cover 8. An ice box 802 is installed on the inner side of the storage box. Six placement grooves 901 are opened on the inner side of the ice box. The placement grooves 901 are cylindrical placement grooves. The diameter of the storage box placement grooves 901 is slightly larger than the diameter of the storage tube, and the diameter of the storage box placement grooves is slightly smaller than the diameter of the tube cover 1 of the storage tube.

[0026] The cell storage and transplantation device for cell transplantation under the renal capsule of small animals of the present invention is used as follows: add the cell suspension to be transplanted into the inner tube 5 of the storage tube, cover the tube cover 1 of the outer tube 6 of the storage tube, put it into a low-speed centrifuge for centrifugation, let the cells settle to the lower section 402 of the storage tube, and then put the storage tube into the matching storage ice box placement slot 901 for storage or transportation. When transplanting cells, take out the storage tube in the storage ice box placement slot 901, open the outer tube cover 1 of the storage tube, use it with a 10μL pipette, press the pressure device of the pipette, empty the air in the gun body, insert the inner tube connecting section 501 of the storage tube, release the pressure device, use the pipette to connect and take out the inner tube 5, peel off the end gel circular closed end 504 of the lower section 503 of the inner tube 5, due to the sealing property of the pipette and the effect of atmospheric pressure, the cells in the lower section 503 of the inner tube 5 will not flow out by themselves due to the peeling off of the end gel circular closed end 504. When in use, use a pipette to insert the end of the lower section 503 of the inner tube 5 into the front end below the renal capsule of a small animal, then press the pressure device of the pipette to give a uniform pressure so that the cells flow out of the lower section 503 of the inner tube 5 at a uniform speed due to atmospheric pressure.

[0027] The above description is only a preferred embodiment of the present invention and is not intended to impose any limitation on the technical solution of the present invention. Those skilled in the art should understand that, without departing from the spirit and principles of the present invention, the technical solution can also be subjected to several simple modifications and substitutions, and these modifications and substitutions are also within the scope of protection covered by the claims.

Claims

1. A cell storage and transplantation device for cell transplantation under the renal capsule of small animals, characterized in that , including two major parts: a storage tube and a storage box: the storage tube includes an outer tube and an inner tube, the outer tube includes a tube cover and a tube body, a circular cotton core is installed on the upper side of the inner part of the tube cover, the cotton core matches the size of the inner tube connecting section, a circular hole groove is set at the tube mouth of the outer tube, the outer tube body includes an upper section and a lower section, the upper section is a cylindrical tube, the lower section is a conical tube with a larger upper part and a smaller lower part, the upper section is connected to the large end of the conical tube of the lower section, and the small end of the conical tube of the lower section is a flat bottom; the inner tube of the storage tube includes a connecting section and a lower part for storing cells, the connecting section is adapted to the size of the connecting part of a 10μL pipette, the lower part of the storage cells includes an upper section and a lower section, the upper section is a conical tube with a larger upper part and a smaller lower part, the lower section is a cylindrical tube, the small end of the conical tube of the upper section is connected to the lower section, and the terminal end of the lower section is provided with a circular closed end of gel. A box cover is installed on the top of the storage box, a fixing pad is fixedly installed on the inner top end of the box cover, an ice box is installed on the inner side of the storage box, and six placement grooves are opened on the inner side of the ice box, and the placement grooves are cylindrical placement grooves.

2. A cell storage and transplantation device for cell transplantation under the renal capsule of small animals according to claim 1, characterized in that The diameter of the circular hole groove at the outer tube opening is slightly smaller than the diameter of the inner tube connecting section of the storage tube.

3. A cell storage and transplantation device for cell transplantation under the renal capsule of small animals according to claim 1, characterized in that , the length of the outer tube and the inner tube is 60-70mm.

4. A cell storage and transplantation device for cell transplantation under the renal capsule of small animals according to claim 1, characterized in that , the upper section of the outer tube and the lower section of the outer tube are integrally formed.

5. A cell storage and transplantation device for cell transplantation under the renal capsule of small animals according to claim 1, characterized in that , the storage tube inner tube connecting section, the lower section of the storage inner tube and the end gel circular closed end of the lower section of the storage inner tube are integrally formed.

6. A cell storage and transplantation device for cell transplantation under the renal capsule of small animals according to claim 1, characterized in that The diameter of the storage box placement groove is slightly larger than the diameter of the storage tube, and the diameter of the storage box placement groove is slightly smaller than the diameter of the tube cover of the storage tube.

7. A cell storage and transplantation device for cell transplantation under the renal capsule of small animals according to claim 1, characterized in that The round closed end of the gel at the lower section of the storage inner tube is easy to remove without leaving any residual glue.

8. A cell storage and transplantation device for cell transplantation under the renal capsule of small animals according to claim 1, characterized in that The inner tube and the outer tube of the storage tube are both sterilized at high temperature and can be directly used to store cells.

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